Neves, R. J., J. R. Weaver and A. V. Zale (1985). An evaluation of host fish suitability for glochidia of Villosa vanuxemi and V. nebulosa (Pelecypoda: Unionidae). American Midland Naturalist. 113:13-19. Fish species congeneric with previously identified hosts, as well as exotic fishes (Xiphophorus variatus and Tilapia aurea) and the mosquitofish Gambusia affinis, were exposed to glochidia of Villosa vanuxemi or V. nebulosa to determine whether phytogenetically similar fishes can serve as hosts. Glochidia of V. vanuxemi metamorphosed on black Cottus baileyi, mottled C. bairdi and slimy C. cognatus sculpins, and glochidia of V. nebulosa metamosphosed on spotted Micropterus punctulatus, largemouth M. samoides and Suwannee M. notius basses and the mosquitofish. Exotic fishes were unsuitable hosts. A review of previous in vivo and in vitro studies suggests that chemical components of the blood serum in fishes, as yet unidentified, dictate host suitability to specific glochidia.