Soares mouse 3NbMS

Library Description

Organism:	Mus musculus
Strain:	C57BL/6J
Developmental stage:	4 weeks
Sex:	male
Tissue:	Spleen
Vector:	pT7T3D-PacI
Vector type:	phagemid (ampicillin resistant)
Cloning site 1:	Not I
Cloning site 2:	Eco RI
Lab host:	DH10B

1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through three rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

Source: Marra M/Mouse EST Project, Washington University School of MedicineP

Gene Content Analysis

29,239 ESTs from this library were grouped into 8,798 UniGene entries (putative genes) [UniGene build #177, 29-Nov-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
78	2668	Mm.472787	Interferon activated gene 203 (Ifi203), transcript variant 2, mRNA.
65	2223	Mm.342177	Immunoglobulin heavy constant gamma 1 (G1m marker), mRNA (cDNA clone MGC:28019 IMAGE:3660622).
63	2155	Mm.4046	Membrane-spanning 4-domains, subfamily A, member 1, mRNA (cDNA clone MGC:36191 IMAGE:4503841).
61	2086	Mm.392203	Selenoprotein P, plasma, 1, mRNA (cDNA clone MGC:5722 IMAGE:3486761).
61	2086	Mm.261270	Interferon activated gene 203, mRNA (cDNA clone MGC:5842 IMAGE:3257492).
53	1813	Mm.295578	Tripartite motif-containing 30, mRNA (cDNA clone MGC:6159 IMAGE:3493256).
52	1778	Mm.219621	Endothelial ligand for L-selectin (GLYCAM1).
48	1642	Mm.30478	Ribosomal protein L39 (Rpl39), mRNA.
48	1642	Mm.138449	PREDICTED: Mus musculus similar to Interferon-activatable protein 203 (Ifi-203) (Interferon-inducible protein p203) (LOC623121), mRNA.
47	1607	Mm.458003	Immunoglobulin heavy chain complex, mRNA (cDNA clone MGC:30284 IMAGE:4205959).
47	1607	Mm.283293	Transmembrane protein 123 (Tmem123), mRNA.
46	1573	Mm.196013	Sterile alpha motif domain containing 9-like, mRNA (cDNA clone IMAGE:4976128).
44	1505	Mm.1192	Immunoglobulin joining chain (Igj), mRNA.
43	1471	Mm.6381	Small secreted protein interferon-induced.
43	1471	Mm.391573	Protein tyrosine phosphatase, receptor type, C (Ptprc), transcript variant 2, mRNA.
39	1334	Mm.155896	Heterogeneous nuclear ribonucleoprotein A2/B1 (Hnrpa2b1).
38	1300	Mm.316861	CD53 antigen, mRNA (cDNA clone MGC:29192 IMAGE:5010267).
38	1300	Mm.153911	Lymphocyte cytosolic protein 1, mRNA (cDNA clone MGC:30234 IMAGE:3486993).
36	1231	Mm.333124	CDNA clone MGC:27817 IMAGE:3482714.
36	1231	Mm.10116	Chemokine (C-X-C motif) ligand 13, mRNA (cDNA clone MGC:13851 IMAGE:4217539).
35	1197	Mm.248478	SAM domain and HD domain, 1, mRNA (cDNA clone MGC:14068 IMAGE:4037046).
34	1163	Mm.163	Beta-2 microglobulin mRNA, segment 1, clones pBRcB-(1-3)..
33	1129	Mm.269815	CD164 antigen, mRNA (cDNA clone MGC:5693 IMAGE:3600535).
32	1094	Mm.471928	Transcribed locus.
32	1094	Mm.458030	Transcribed locus, strongly similar to NP_904330.1 cytochrome c oxidase subunit I [Mus musculus].
32	1094	Mm.24769	Interferon gamma inducible protein 47, mRNA (cDNA clone MGC:11403 IMAGE:2651113).
32	1094	Mm.218473	Serine incorporator 3, mRNA (cDNA clone MGC:31028 IMAGE:5039323).
31	1060	Mm.45740	Guanylate binding protein 6 (Gbp6), mRNA.
31	1060	Mm.371956	Predicted gene, OTTMUSG00000016644 (OTTMUSG00000016644), transcript variant 2, mRNA.
31	1060	Mm.355034	Transmembrane protein 30A, mRNA (cDNA clone IMAGE:3669745).
30	1026	Mm.392724	Transcribed locus.
30	1026	Mm.331964	DNA segment, Chr 4, Wayne State University 53, expressed (D4Wsu53e), mRNA.
30	1026	Mm.288974	Actin related protein 2/3 complex, subunit 5 (Arpc5), mRNA.
30	1026	Mm.200362	Cytochrome b-245, beta polypeptide (Cybb), mRNA.
30	1026	Mm.1761	CDC-like kinase 1 (Clk1), transcript variant 2, mRNA.
30	1026	Mm.138876	Moesin (Msn), mRNA.
29	992	Mm.249555	Collagen, type III, alpha 1, mRNA (cDNA clone IMAGE:2812464).
28	958	Mm.392504	Capping protein (actin filament) muscle Z-line, alpha 2 (Capza2), mRNA.
28	958	Mm.273379	Sorting nexin 5 (Snx5), mRNA.
28	958	Mm.25559	Serine/threonine kinase 17b (apoptosis-inducing) (Stk17b), mRNA.
28	958	Mm.2018	Core binding factor beta, mRNA (cDNA clone MGC:30918 IMAGE:4016060).
28	958	Mm.101591	CD55 antigen, mRNA (cDNA clone MGC:18419 IMAGE:3663561).
26	889	Mm.447910	Transcribed locus, moderately similar to NP_904330.1 cytochrome c oxidase subunit I [Mus musculus].
25	855	Mm.425473	PREDICTED: Mus musculus similar to very large inducible GTPase-1, transcript variant 1 (LOC100042856), mRNA.
25	855	Mm.297482	Tumor protein, translationally-controlled 1 (Tpt1), mRNA.
25	855	Mm.220038	DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, mRNA (cDNA clone IMAGE:3598655).
24	821	Mm.475151	Transcribed locus.
24	821	Mm.473693	ASF mRNA.
24	821	Mm.333349	WNK lysine deficient protein kinase 1, mRNA (cDNA clone IMAGE:5371931).
24	821	Mm.327358	Histocompatibility 2, O region beta locus (H2-Ob), mRNA.
24	821	Mm.313133	CDNA clone IMAGE:30303372.
24	821	Mm.30059	Myristoylated alanine rich protein kinase C substrate (Marcks), mRNA.
24	821	Mm.260084	Eukaryotic translation initiation factor 4A2 (Eif4a2), transcript variant 1, mRNA.
23	787	Mm.68134	Epithelial stromal interaction 1 (breast), mRNA (cDNA clone MGC:28189 IMAGE:3988503).
23	787	Mm.326945	Tripartite motif-containing 34 (Trim34), mRNA.
23	787	Mm.289630	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (Ywhaq), mRNA.
23	787	Mm.260635	Membrane-associated ring finger (C3HC4) 7, mRNA (cDNA clone MGC:35882 IMAGE:4935124).
23	787	Mm.198803	UDP-glucose ceramide glucosyltransferase (Ugcg), mRNA.
23	787	Mm.193099	Fibronectin 1, mRNA (cDNA clone IMAGE:4985138).
23	787	Mm.185453	CDNA clone IMAGE:3598196.
23	787	Mm.1738	CD48 antigen (Cd48), mRNA.
23	787	Mm.1643	RIKEN cDNA 4632417K18 gene, mRNA (cDNA clone MGC:46762 IMAGE:3599082).
22	752	Mm.56769	Decorin (Dcn), mRNA.
22	752	Mm.440802	DNA segment, Chr 12, ERATO Doi 553, expressed, mRNA (cDNA clone MGC:63391 IMAGE:6834768).
22	752	Mm.440525	Guanylate binding protein 4 (Gbp4), mRNA.
22	752	Mm.290906	Nuclear antigen Sp100 (Sp100), mRNA.
22	752	Mm.277792	Collagen, type I, alpha 2, mRNA (cDNA clone IMAGE:3481648).
22	752	Mm.219527	Complement component 1, s subcomponent (C1s), transcript variant 1, mRNA.
21	718	Mm.439751	Interferon-induced protein with tetratricopeptide repeats 1, mRNA (cDNA clone MGC:5959 IMAGE:3496673).
21	718	Mm.401648	RAN binding protein 2 (Ranbp2), mRNA.
21	718	Mm.38888	Serine (or cysteine) peptidase inhibitor, clade G, member 1, mRNA (cDNA clone MGC:5908 IMAGE:3485810).
21	718	Mm.341243	Pumilio 2 (Drosophila), mRNA (cDNA clone IMAGE:3595161).
21	718	Mm.33957	Membrane-spanning 4-domains, subfamily A, member 4B, mRNA (cDNA clone MGC:41078 IMAGE:1248222).
21	718	Mm.275710	Tetratricopeptide repeat domain 14 (Ttc14), transcript variant 1, mRNA.
21	718	Mm.271222	Eukaryotic translation initiation factor 5, mRNA (cDNA clone MGC:36374 IMAGE:4984912).
21	718	Mm.206841	SMC4 structural maintenance of chromosomes 4-like 1 (yeast), mRNA (cDNA clone IMAGE:3589989).
21	718	Mm.1355	CD79A antigen (immunoglobulin-associated alpha), mRNA (cDNA clone MGC:41188 IMAGE:1332396).
21	718	Mm.12665	Cullin 3, mRNA (cDNA clone MGC:28933 IMAGE:3981170).
20	684	Mm.86514	GTPase, IMAP family member 8, mRNA (cDNA clone MGC:169567 IMAGE:8860962).
20	684	Mm.86391	Apolipoprotein L 7c, mRNA (cDNA clone IMAGE:1332393).
20	684	Mm.447	Pyrin and HIN domain family, member 1, mRNA (cDNA clone MGC:130358 IMAGE:40056703).
20	684	Mm.4266	Integral membrane protein 2B, mRNA (cDNA clone MGC:27534 IMAGE:4458918).
20	684	Mm.3999	MPS1 gene and mRNA, 3'end.
20	684	Mm.390865	CD47 antigen (Rh-related antigen, integrin-associated signal transducer), mRNA (cDNA clone MGC:13838 IMAGE:4187965).
20	684	Mm.355614	CAMP phosphodiesterase 7A1.
20	684	Mm.33764	Influenza virus NS1A binding protein (Ivns1abp), transcript variant 2, mRNA.
20	684	Mm.331640	Transcribed locus.
20	684	Mm.291542	RNA binding motif protein 26, mRNA (cDNA clone IMAGE:3490473).
20	684	Mm.278922	Far upstream element (FUSE) binding protein 1 (Fubp1), mRNA.
20	684	Mm.24038	Guanylate binding protein 2, mRNA (cDNA clone MGC:41173 IMAGE:1230883).
20	684	Mm.210352	Splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (Sfrs10), mRNA.
20	684	Mm.198264	RAB14, member RAS oncogene family, mRNA (cDNA clone MGC:36272 IMAGE:3980228).
19	650	Mm.471741	Transcribed locus.
19	650	Mm.46005	RNA binding motif protein 25 (Rbm25), mRNA.
19	650	Mm.440719	PREDICTED: Mus musculus predicted gene, EG666926 (EG666926), mRNA.
19	650	Mm.436639	Membrane-spanning 4-domains, subfamily A, member 6B, mRNA (cDNA clone MGC:35625 IMAGE:3984299).
19	650	Mm.4360	CD19 antigen (Cd19), mRNA.
19	650	Mm.38199	B and T lymphocyte associated (Btla), transcript variant 1, mRNA.
19	650	Mm.297393	PREDICTED: Mus musculus hect domain and RLD 5, transcript variant 1 (Herc5), mRNA.
19	650	Mm.291442	Secreted acidic cysteine rich glycoprotein, mRNA (cDNA clone MGC:6232 IMAGE:3586402).