Gene ID:TDE0714
DNA Molecule Name:
1
GenBank ID:
42526228
Gene Name:
cap5D cap8D capD
Definition:
capsular polysaccharide biosynthesis protein
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
747773
Gene Stop:
749257
Gene Length:
1485
Molecular Weight*:
55573
pI*:
Net Charge*:
EC:
Functional Class:
Cell envelope; Surface polysaccharides, lipopolysaccharides and antigens
Gene Ontology:
Pathway: pathway table
Secondary Evidence:
Makino S, Watarai M, Cheun HI, Shirahata T, Uchida I.
Effect of the lower molecular capsule released from the cell surface of Bacillus anthracis on the pathogenesis of anthrax.
J Infect Dis. 2002 Jul;186(2):227-33.
PMID: 12134259
Wood AC, Oldfield NJ, O'Dwyer CA, Ketley JM.
Cloning, mutation and distribution of a putative lipopolysaccharide biosynthesis locus in Campylobacter jejuni.
Microbiology. 1999 Feb;145 ( Pt 2):379-88.
PMID: 10075420
Comment:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
BeTs to clades of COG1086
COG name: Predicted nucleoside-diphosphate sugar epimerases
Functional Class: M,G
The phylogenetic pattern of COG1086 is --m-----v---b--fghsnujx-t-
Number of proteins in this genome belonging to this COG is
Blocks Summary: Blocks Search
Significant hit ( 2.7e-18) to 2/5 blocks of the IPB001509 family, which is described as "NAD dependent epimerase/dehydratase family". Interpro entry for IPR001509.
IPB001509A 151-172 1.5e-05
IPB001509B 230-264 3.9e-11
ProDom Summary: Protein Domain Search
Residues 3 to 228 match (2e-82) PD:PD005861 which is described as COMPLETE PROTEOME BIOSYNTHESIS EPIMERASE/DEHYDRATASE POLYSACCHARIDE SUGAR CAPSULAR TRANSFERASE WBPM PLASMID
Residues 279 to 441 match (3e-60) PD:PD004112 which is described as PROTEOME COMPLETE EPIMERASE/DEHYDRATASE BIOSYNTHESIS POLYSACCHARIDE CAPSULAR SUGAR NUCLEOTIDE CAPD WBPM
Residues 229 to 278 match (3e-17) PD:PD000038 which is described as PROTEOME COMPLETE 4-EPIMERASE UDP-GLUCOSE REDUCTASE 46-DEHYDRATASE GALACTOSE NAD DEHYDROGENASE DTDP-GLUCOSE
Paralogs: Local Blast Search
Pfam Summary: Pfam Search
Residues 1 to 486 (E-value = 1.1e-201) place TDE0714 in the Polysacc_synt_2 family which is described as Polysaccharide biosynthesis protein (PF02719)
Residues 147 to 416 (E-value = 6.2e-07) place TDE0714 in the 3Beta_HSD family which is described as 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073)
Residues 151 to 446 (E-value = 3.4e-09) place TDE0714 in the Epimerase family which is described as NAD dependent epimerase/dehydratase family (PF01370)
Top PDB Hits:
PDB hits to TDE0714 from Psi-BLAST round 5 vs. nr database
19.7% similar to PDB:1BXK Dtdp-Glucose 4,6-Dehydratase From E. Coli (Chain A,B; 7e-95)
18.5% similar to PDB:1G1A The Crystal Structure Of Dtdp-D-Glucose 4,6-Dehydratase (Rmlb)from Salmonella En (Chain A,B,C,D; 4e-93)
18.5% similar to PDB:1KEU The Crystal Structure Of Dtdp-D-Glucose 4,6-Dehydratase (Rmlb) From Salmonella E (Chain A,B; 4e-93)
18.5% similar to PDB:1KEW The Crystal Structure Of Dtdp-D-Glucose 4,6-Dehydratase (Rmlb) From Salmonella E (Chain A,B; 4e-93)
23.1% similar to PDB:1R66 Crystal Structure Of Desiv (Dtdp-Glucose 4,6-Dehydratase) From Streptomyces Vene (Chain A; 8e-89)
22.8% similar to PDB:1R6D Crystal Structure Of Desiv Double Mutant (Dtdp-Glucose 4,6- Dehydratase) From St (Chain A; 3e-87)
19.9% similar to PDB:1KVS Udp-Galactose 4-Epimerase Complexed With Udp-Phenol (5e-87)
19.6% similar to PDB:1UDC Structure Of Udp-Galactose-4-Epimerase Complexed With Udp-Mannose (8e-87)
19.6% similar to PDB:2UDP Udp-Galactose 4-Epimerase Complexed With Udp-Phenol (Chain A,B; 8e-87)
19.6% similar to PDB:1XEL Udp-Galactose 4-Epimerase From Escherichia Coli (8e-87)
Gene Protein Sequence:
MNRPIKQKTSIYIVGAGLAGTMIAHEISEKNIFGKVAAFLDDDPKKIGTK
IDGIPVFGPISETVHLIRIDAGDEALIAIPSMRSERLREIYELLKSAGFS
KIKLLPAISQIIDGSAHLVQAREIDPQDLLTRTPVTISLKKSLAYLRGKR
VLITGAGGSIGSELCRQLLSGGAERLYLFGHGENSIYQIYKELKILQAGG
VGDKATIVPVIGELKDREYMRYIIKQLKCDAVFHTAAYKHVPLMEENPVA
VIENNVFGTKNLLDACIEFGVKRFVLISTDKAVEPVSVYGVSKLLSEKLV
LQAAETVKEKKDSAYMFVRFGNVLGSRGSIFPLFVEQIQNGGPVTVTDKK
MIRFFMTIPEACSLVLQTGGVGKSGESYLLDMGEPVNIYETAKQLISYMG
FEPEKDIKIEIIGPREGERLEEPLWSSTEYLEKTDYEKIMRLRDKKEFDS
KNLNEILNTLYPFCFYSEEHKDDFRNKEKMRNFLEENKLLIYKKE
Gene Nucleotide Sequence: Sequence Viewer
ATGAACCGTCCTATTAAACAAAAAACTTCTATATATATAGTAGGCGCAGG
TCTGGCCGGAACTATGATTGCACATGAAATTTCTGAAAAAAACATATTCG
GTAAGGTTGCCGCATTCTTAGACGATGACCCTAAAAAAATCGGAACTAAG
ATTGACGGGATTCCGGTATTCGGGCCTATAAGTGAAACGGTTCATCTGAT
AAGGATAGATGCAGGGGATGAGGCTCTGATTGCAATTCCCAGCATGCGCT
CCGAGCGTTTGCGTGAAATATATGAGCTTTTAAAATCGGCAGGTTTTTCA
AAAATAAAACTTCTGCCCGCAATTTCGCAAATCATTGACGGTTCAGCTCA
TTTGGTACAAGCCCGCGAAATAGATCCGCAGGATTTACTTACCCGAACCC
CCGTTACCATTTCTTTAAAAAAGAGTTTGGCTTATCTACGCGGAAAGCGA
GTTCTAATTACCGGCGCCGGAGGTTCTATCGGAAGCGAACTCTGCCGGCA
GCTTTTATCGGGCGGTGCCGAACGCCTCTATCTTTTCGGTCATGGAGAAA
ATTCCATTTATCAAATTTATAAAGAACTTAAAATATTGCAAGCAGGCGGT
GTCGGCGACAAGGCTACAATTGTTCCGGTTATAGGAGAGTTAAAAGACCG
CGAGTACATGCGCTACATCATTAAGCAATTAAAATGTGATGCGGTTTTTC
ATACGGCGGCTTATAAACATGTTCCTTTGATGGAAGAAAATCCTGTTGCG
GTTATCGAAAACAATGTTTTCGGAACAAAGAATCTTTTGGATGCCTGCAT
CGAATTCGGAGTAAAACGATTTGTTTTAATTTCGACGGATAAGGCTGTTG
AGCCTGTTTCGGTTTACGGCGTTTCAAAACTATTAAGCGAAAAATTGGTA
TTGCAGGCTGCCGAAACCGTAAAAGAAAAAAAAGATTCAGCCTACATGTT
TGTCCGCTTCGGAAATGTTTTGGGATCGAGGGGATCTATCTTTCCGCTTT
TTGTAGAGCAGATACAAAACGGGGGACCCGTAACCGTAACCGATAAAAAG
ATGATCCGCTTTTTTATGACAATTCCGGAAGCTTGTTCATTAGTATTGCA
GACAGGCGGTGTTGGAAAATCGGGTGAGTCCTATCTTCTCGACATGGGAG
AACCTGTAAATATTTATGAGACGGCAAAGCAGTTGATAAGTTACATGGGT
TTTGAACCTGAGAAGGATATTAAGATAGAAATAATCGGCCCCAGAGAAGG
CGAGCGTTTGGAAGAGCCTCTTTGGTCATCAACCGAGTATCTTGAAAAAA
CGGATTACGAAAAAATCATGCGCTTACGTGATAAAAAAGAATTCGATTCT
AAAAACTTAAATGAAATTTTAAATACCCTATATCCGTTTTGTTTTTATAG
TGAAGAACACAAAGACGATTTCCGCAATAAAGAAAAGATGCGCAATTTTT
TGGAAGAAAATAAATTATTAATTTATAAAAAGGAG