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Library:10301 (dbEST ID)
S5SNU484
Library Description
| Organism: | Homo sapiens |
| Sex: | M |
| Organ: | Stomach |
| Tissue: | Stomach |
| Cell type: | Epithelial |
| Cell line: | SNU-484 |
| Vector: | pTZ18RP1 |
| Vector type: | Plasmid |
| Cloning site 1: | EcoRI |
| Cloning site 2: | NotI |
| Lab host: | Top10F' |
The poly (A)+ RNA was decapped with tabacco acid pyrophosphatase (TAP) and ligated with DNA-RNA linker including EcoRI site by treatment of T4 RNA ligase. The first strand cDNA was synthesized from oligo dT-selected mRNA by priming with dT-tailed vector. The dT-tailed vector was adjusted to have about 60nt. The cDNA vector was circularized with E. coli DNA ligase after digestion of EcoRI which site is also included in vector. An RNA strand converted to a DNA strand by Okayama-Berg method. The obtained cDNA vectors were used for transformation of competent cells E. coli Top10F' by electroporation method.
Source: Kim YS, Korea Research Institute of Bioscience & Biotechnology
Gene Content Analysis
2,409 ESTs from this library were grouped into 991 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 5 | 2076 |  |
Hs.44276 | HOXC10 protein, exon 2. |
| 5 | 2076 | |
Hs.441113 | MAGE-6 protein. |
| 5 | 2076 | |
Hs.433499 | Cementum protein 1, mRNA (cDNA clone MGC:182028 IMAGE:9056853). |
| 5 | 2076 | |
Hs.419640 | RNA-binding protein regulatory subunit. |
| 5 | 2076 | |
Hs.381219 | CDNA FLJ51497 complete cds, highly similar to 60S ribosomal protein L15. |
| 5 | 2076 | |
Hs.356572 | (clone 03) liver expessed ribosomal protein S3 homologue. |
| 5 | 2076 | |
Hs.273330 | Agrin precursor. |
| 5 | 2076 | |
Hs.270869 | Clones 23667 and 23775 zinc finger protein. |
| 5 | 2076 | |
Hs.195080 | Homo sapiens, clone IMAGE:5446468, mRNA. |
| 5 | 2076 | |
Hs.170622 | CDNA PSEC0145 fis, clone PLACE1006711, highly similar to EGF-containing fibulin-like extracellular matrix protein 2 precursor. |
| 5 | 2076 | |
Hs.108371 | E2F-4=transcription factor [human, Nalm6 and HeLa cells, mRNA, 1539 nt]. |
| 4 | 1660 | |
Hs.91579 | Clone 23783 mRNA sequence. |
| 4 | 1660 | |
Hs.77628 | CAB1. |
| 4 | 1660 | |
Hs.74137 | (clone S31i125) mRNA, 3' end of cds. |
| 4 | 1660 | |
Hs.714327 | PREDICTED: Homo sapiens similar to hCG1778032 (LOC100130711), mRNA. |
| 4 | 1660 | |
Hs.712563 | CDNA FLJ56876 complete cds, highly similar to Nuclear envelope pore membrane protein POM 121. |
| 4 | 1660 | |
Hs.707755 | Transcribed locus. |
| 4 | 1660 | |
Hs.677849 | Transcribed locus. |
| 4 | 1660 | |
Hs.648395 | Hypothetical protein LOC339047, mRNA (cDNA clone MGC:47688 IMAGE:4479933). |
| 4 | 1660 | |
Hs.601732 | Transcribed locus. |
| 4 | 1660 | |
Hs.592290 | CDNA FLJ90412 fis, clone NT2RP3000059, weakly similar to ANKYRIN, BRAIN VARIANT 2. |
| 4 | 1660 | |
Hs.534770 | Opa-interacting protein OIP3. |
| 4 | 1660 | |
Hs.534404 | Laminin receptor homolog {3' region} [human, mRNA Partial, 739 nt]. |
| 4 | 1660 | |
Hs.534312 | TorsinA (DYT1). |
| 4 | 1660 | |
Hs.533192 | KIAA0016 gene. |
| 4 | 1660 | |
Hs.525752 | Kruppel-like factor 13, mRNA (cDNA clone IMAGE:3943625). |
| 4 | 1660 | |
Hs.523936 | PRCP mRNA for prolylcarboxypeptidase isoform 1 preproprotein, complete cds, clone: FLJ08097AAAN. |
| 4 | 1660 | |
Hs.516954 | ARHGEF11/KIAA0380 mRNA for KIAA0380 protein. |
| 4 | 1660 | |
Hs.516826 | CDNA FLJ59723 complete cds, highly similar to Tribbles homolog 3. |
| 4 | 1660 | |
Hs.514870 | H+-ATP synthase subunit b. |
| 4 | 1660 | |
Hs.514216 | Homo sapiens, clone IMAGE:4150824, mRNA. |
| 4 | 1660 | |
Hs.504609 | Id1 (Id1-a)=transcription regulator helix-loop-helix protein {alternatively spliced} [human, glioblastoma cell line U251, mRNA Partial, 530 nt]. |
| 4 | 1660 | |
Hs.502842 | CDNA FLJ53224 complete cds, highly similar to Calpain-1 catalytic subunit (EC 3.4.22.52). |
| 4 | 1660 | |
Hs.498732 | Peroxisomal phytanoyl-CoA alpha-hydroxylase (PAHX). |
| 4 | 1660 | |
Hs.473788 | CDNA FLJ61195 complete cds, highly similar to Ubiquitin thioesterase protein OTUB1 (EC 3.4.-.-). |
| 4 | 1660 | |
Hs.443061 | CDNA FLJ31785 fis, clone NT2RI2008502. |
| 4 | 1660 | |
Hs.432674 | HeLa mRNA isolated as a false positive in a two-hybrid-screen. |
| 4 | 1660 | |
Hs.416090 | CDNA FLJ59181 complete cds, highly similar to Receptor expression-enhancing protein 2. |
| 4 | 1660 | |
Hs.37107 | MAGE-41 protein. |
| 4 | 1660 | |
Hs.369279 | NLR family, pyrin domain containing 2, mRNA (cDNA clone MGC:33950 IMAGE:5298283). |
| 4 | 1660 | |
Hs.362817 | CDNA FLJ59007 complete cds, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. |
| 4 | 1660 | |
Hs.33642 | Archain 1 (ARCN1), transcript variant 2, mRNA. |
| 4 | 1660 | |
Hs.323342 | Arp2/3 protein complex subunit p20-Arc (ARC20). |
| 4 | 1660 | |
Hs.286221 | Clone 24614 ADP-ribosylation factor 1. |
| 4 | 1660 | |
Hs.246112 | Small nuclear ribonucleoprotein 200kDa (U5) (SNRNP200), mRNA. |
| 4 | 1660 | |
Hs.226499 | Homo sapiens, Similar to nesca protein, clone IMAGE:5108891, mRNA. |
| 4 | 1660 | |
Hs.205163 | MRL3 mRNA for ribosomal protein L3 homologue ( MRL3 = mammalian ribosome L3 ). |
| 4 | 1660 | |
Hs.200600 | Secretory carrier membrane protein (SCAMP3). |
| 4 | 1660 | |
Hs.181163 | High-mobility group nucleosomal binding domain 2, mRNA (cDNA clone MGC:29711 IMAGE:5021546). |
| 4 | 1660 | |
Hs.132497 | Clone 23856 unknown. |
| 4 | 1660 | |
Hs.129673 | Eukaryotic initiation factor 4AI. |
| 4 | 1660 | |
Hs.113094 | CDNA FLJ76968 complete cds, highly similar to Homo sapiens coronin, actin binding protein, 2A (CORO2A), transcript variant 2, mRNA. |
| 3 | 1245 |  |
Hs.8102 | Ribosomal protein S20, 5'UTR region. |
| 3 | 1245 | |
Hs.78788 | Clone 23772 mRNA sequence. |
| 3 | 1245 | |
Hs.7753 | Calumenin. |
| 3 | 1245 | |
Hs.75864 | GP36b glycoprotein. |
| 3 | 1245 | |
Hs.75682 | Autoantigen. |
| 3 | 1245 | |
Hs.716384 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2), mRNA (cDNA clone MGC:47616 IMAGE:5553465). |
| 3 | 1245 | |
Hs.710584 | Transcribed locus. |
| 3 | 1245 | |
Hs.709383 | KIAA1191, mRNA (cDNA clone MGC:14952 IMAGE:3529175). |
| 3 | 1245 | |
Hs.706341 | Transcribed locus, moderately similar to XP_001722209.1 PREDICTED: hypothetical protein [Homo sapiens]. |
| 3 | 1245 | |
Hs.706229 | Transcribed locus, strongly similar to NP_033739.1 actin, gamma, cytoplasmic 1 [Mus musculus]. |
| 3 | 1245 | |
Hs.677637 | Transcribed locus. |
| 3 | 1245 | |
Hs.66762 | Family with sequence similarity 171, member A1, mRNA (cDNA clone IMAGE:5276340). |
| 3 | 1245 | |
Hs.655193 | Fibroblast growth factor homologous factor 3 (FHF-3). |
| 3 | 1245 | |
Hs.654404 | MHC class I human leukocyte antigen, allele HLA-Cw*12042. |
| 3 | 1245 | |
Hs.632380 | UDP-galactose-4-epimerase (GALE), transcript variant 3, mRNA. |
| 3 | 1245 | |
Hs.627414 | Ribosomal protein S18 (RPS18), mRNA. |
| 3 | 1245 | |
Hs.618713 | CDNA FLJ37839 fis, clone BRSSN2011653, highly similar to RNA-binding protein 4B. |
| 3 | 1245 | |
Hs.61184 | RNA binding motif protein, X-linked 2, mRNA (cDNA clone IMAGE:3686686). |
| 3 | 1245 | |
Hs.600975 | Transcribed locus. |
| 3 | 1245 | |
Hs.592121 | Rabaptin, RAB GTPase binding effector protein 1, mRNA (cDNA clone MGC:48839 IMAGE:6046320). |
| 3 | 1245 | |
Hs.591019 | Beta-1,4 N-acetylgalactosaminyltransferase. |
| 3 | 1245 | |
Hs.588289 | Protein-serine/threonine kinase gene. |
| 3 | 1245 | |
Hs.546449 | MRNA; cDNA DKFZp434O172 (from clone DKFZp434O172). |
| 3 | 1245 | |
Hs.534350 | MRNA full length insert cDNA clone EUROIMAGE 1160430. |
| 3 | 1245 | |
Hs.532359 | Ribosomal protein L5. |
| 3 | 1245 | |
Hs.524464 | P2 mRNA for ATP synthase subunit c. |
| 3 | 1245 | |
Hs.523238 | Nucleolar phosphoprotein p130. |
| 3 | 1245 | |
Hs.518562 | CDNA FLJ51563 complete cds. |
| 3 | 1245 | |
Hs.517949 | Microtubule-associated protein 4 (MAP4). |
| 3 | 1245 | |
Hs.516087 | CDNA FLJ90359 fis, clone NT2RP2003655, highly similar to Protein TEX261. |
| 3 | 1245 | |
Hs.508829 | CDNA clone IMAGE:3641010. |
| 3 | 1245 | |
Hs.502328 | CDw44 antigen. |
| 3 | 1245 | |
Hs.501522 | Methyltransferase. |
| 3 | 1245 | |
Hs.477498 | Eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC), mRNA. |
| 3 | 1245 | |
Hs.477361 | CDNA FLJ33025 fis, clone THYMU1000558, highly similar to Homo sapiens SEC22 vesicle trafficking protein-like 2 (S. cerevisiae) (SEC22L2), mRNA. |
| 3 | 1245 | |
Hs.473648 | GARS-AIRS-GART. |
| 3 | 1245 | |
Hs.470804 | Ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast), mRNA (cDNA clone MGC:1316 IMAGE:3537280). |
| 3 | 1245 | |
Hs.465213 | KIAA0541 protein. |
| 3 | 1245 | |
Hs.449909 | Laminin-binding protein. |
| 3 | 1245 | |
Hs.449114 | Transcribed locus, strongly similar to NP_058580.1 heterogeneous nuclear ribonucleoprotein C [Mus musculus]. |
| 3 | 1245 | |
Hs.446297 | Zinc finger protein 498 (ZNF498), mRNA. |
| 3 | 1245 | |
Hs.445403 | CDNA FLJ55373 complete cds, highly similar to Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5). |
| 3 | 1245 | |
Hs.436500 | CDNA FLJ53677 complete cds, highly similar to Drebrin-like protein. |
| 3 | 1245 | |
Hs.433540 | CDNA FLJ61418 complete cds, highly similar to DnaJ homolog subfamily C member 8. |
| 3 | 1245 | |
Hs.432792 | PREDICTED: Homo sapiens hypothetical protein LOC100128737 (LOC100128737), mRNA. |
| 3 | 1245 | |
Hs.432562 | Clone 23554 mRNA sequence. |
| 3 | 1245 | |
Hs.408623 | MRNA sequence, IMAGE clone 781467. |
| 3 | 1245 | |
Hs.408054 | 60S ribosomal protein L12 (RPL12) pseudogene, partial sequence. |
Source: NCBI UniGene
EST Sequences
2,552 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- stomach
- gastrointestinal tumor
- unknown developmental stage
Source: NCBI Clone Registry