BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'D13B05_C05_03.ab1' (333 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 5 Sequences     : less than 5 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 1120 260 |====================================================
   6310  860 143 |============================
   3980  717 168 |=================================
   2510  549 242 |================================================
   1580  307  91 |==================
   1000  216  99 |===================
    631  117  46 |=========
    398   71  21 |====
    251   50  16 |===
    158   34  15 |===
    100   19   5 |=
   63.1   14   1 |:
   39.8   13   3 |:
   25.1   10   2 |:
   15.8    8   1 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 7  <<<<<<<<<<<<<<<<<
   10.0    7   1 |:
   6.31    6   0 |
   3.98    6   1 |:
   2.51    5   0 |
   1.58    5   0 |
   1.00    5   0 |
   0.63    5   0 |
   0.40    5   1 |:
   0.25    4   0 |
   0.16    4   0 |
   0.10    4   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|7485600|pir||T04014hypothetical protein F17A8.20 -... +1   136  1.5e-07   1
gi|5091625|gb|AAD39613.1|AC007454_12(AC007454) Simila... +1   121  9.6e-06   1
gi|7488933|pir||T14319protein AX110P - carrot >gi|285... +1   105  0.00041   1
gi|12322603|gb|AAG51297.1|AC026480_4(AC026480) oxidor... +1    84  0.068     1
gi|2062334|gb|AAB53349.1|(U57401) unknown [Choristone... +2    50  0.25      2
gi|619390|gb|AAB32148.1|B cell antigen CD20-specific ... -1    60  0.96      1
gi|7516956|pir||F72518hypothetical protein APE2123 - ... +2    68  0.9997    1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|7485600|pir||T04014  hypothetical protein F17A8.20 - Arabidopsis thaliana
            >gi|4538897|emb|CAB39634.1| (AL049482) AX110P-like protein
            [Arabidopsis thaliana] >gi|7267662|emb|CAB78090.1| (AL161515)
            AX110P-like protein [Arabidopsis thaliana]
            Length = 362

Frame  1 hits (HSPs):                   _______                           
                        __________________________________________________
Database sequence:     |                    |                    |        | 362
                       0                  150                  300

  Plus Strand HSPs:

 Score = 136 (47.9 bits), Expect = 1.5e-07, P = 1.5e-07
 Identities = 25/43 (58%), Positives = 33/43 (76%), Frame = +1

Query:    76 ESSGVQFMDSTIWVHNPRTATMAHFLNDAQRFGILKSAFSIHS 204
             E++GVQ MD T+WVHNPRTA +  FL+D++RFG LK+  S  S
Sbjct:   119 EANGVQIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFS 161


to_Entrezto_Relatedto_Related >gi|5091625|gb|AAD39613.1|AC007454_12  (AC007454) Similar to gb|D14605 AX110P
            embryogenesis-associated protein from Daucus carota and is a member
            of the PF|01408 Oxidoreductase family.  ESTs gb|Z35057, gb|T20683
            and gb|Z48399 come from this gene. [Arabidopsis thaliana]
            Length = 450

Frame  1 hits (HSPs):                _____                                
                        __________________________________________________
Database sequence:     |                |                |                | 450
                       0              150              300

  Plus Strand HSPs:

 Score = 121 (42.6 bits), Expect = 9.6e-06, P = 9.6e-06
 Identities = 22/43 (51%), Positives = 30/43 (69%), Frame = +1

Query:    76 ESSGVQFMDSTIWVHNPRTATMAHFLNDAQRFGILKSAFSIHS 204
             E +GVQFMD T W+H+PRT  +  F+ND + FG +KS +S  S
Sbjct:   120 EVNGVQFMDGTQWMHSPRTDKIKEFVNDLESFGQIKSVYSCFS 162


to_Entrezto_Relatedto_Related >gi|7488933|pir||T14319  protein AX110P - carrot >gi|285739|dbj|BAA03455.1|
            (D14605) AX110P [Daucus carota] >gi|740202|prf||2004427A
            embryogenesis-associated protein [Daucus carota]
            Length = 390

Frame  1 hits (HSPs):                  ______                             
                        __________________________________________________
Database sequence:     |                   |                  |           | 390
                       0                 150                300

  Plus Strand HSPs:

 Score = 105 (37.0 bits), Expect = 0.00041, P = 0.00041
 Identities = 19/40 (47%), Positives = 29/40 (72%), Frame = +1

Query:    76 ESSGVQFMDSTIWVHNPRTATMAHFLNDAQRFGILKSAFS 195
             + +GVQ+MD T+  H+PR+A M  +L+DA+ FG L+S  S
Sbjct:   122 DDNGVQYMDGTMLQHHPRSAKMREYLDDAEHFGQLRSIIS 161


to_Entrezto_Relatedto_Related >gi|12322603|gb|AAG51297.1|AC026480_4  (AC026480) oxidoreductase, putative
            [Arabidopsis thaliana]
            Length = 364

Frame  1 hits (HSPs):                   ______                            
                        __________________________________________________
Database sequence:     |                    |                    |        | 364
                       0                  150                  300

  Plus Strand HSPs:

 Score = 84 (29.6 bits), Expect = 0.070, P = 0.068
 Identities = 16/40 (40%), Positives = 25/40 (62%), Frame = +1

Query:    76 ESSGVQFMDSTIWVHNPRTATMAHFLNDAQRFGILKSAFS 195
             E +GVQFMD TIW+H+ RT  +   + D+   G ++  +S
Sbjct:   120 EYNGVQFMDGTIWLHHQRTVKIRDTMFDSGLLGDVRHMYS 159


to_Entrezto_Relatedto_Related >gi|2062334|gb|AAB53349.1|  (U57401) unknown [Choristoneura fumiferana
            nucleopolyhedrovirus]
            Length = 70

Frame  2 hits (HSPs):   ____________________                              
Frame  1 hits (HSPs):                                           _________ 
                        __________________________________________________
Database sequence:     |             |             |              |       | 70
                       0            20            40             60

  Plus Strand HSPs:

 Score = 50 (17.6 bits), Expect = 0.29, Sum P(2) = 0.25
 Identities = 13/29 (44%), Positives = 17/29 (58%), Frame = +2

Query:   107 PSGSITQGLLPWPTSSTMRNVLVSSNRHS 193
             P+  I +G  PWP S T R+  V + RHS
Sbjct:     2 PARHIARGYTPWP-SRTTRDRQVRT-RHS 28

 Score = 37 (13.0 bits), Expect = 0.29, Sum P(2) = 0.25
 Identities = 5/13 (38%), Positives = 11/13 (84%), Frame = +1

Query:   238 QKWLQTLGSVSCH 276
             ++++QT+  V+CH
Sbjct:    57 RRFVQTMRGVACH 69


to_Entrezto_Related >gi|619390|gb|AAB32148.1|  B cell antigen CD20-specific IgG light chain variable
            region {C-terminal} [mice, hybridoma cell line Mem97, Peptide
            Partial, 55 aa]
            Length = 55

Frame -1 hits (HSPs):                     ____________________________    
                        __________________________________________________
Database sequence:     |                 |                 |              | 55
                       0                20                40

  Minus Strand HSPs:

 Score = 60 (21.1 bits), Expect = 3.3, P = 0.96
 Identities = 14/33 (42%), Positives = 16/33 (48%), Frame = -1

Query:   102 IHELHPTGFGNYFAHLTFFWYTQQFFVPGRKAE 4
             I+ L P  FGNY+     FW T   F  G K E
Sbjct:    21 INRLQPEDFGNYYCQ--HFWSTPWTFGGGTKLE 51


to_Entrezto_Relatedto_Related >gi|7516956|pir||F72518  hypothetical protein APE2123 - Aeropyrum pernix (strain
            K1) >gi|5105822|dbj|BAA81134.1| (AP000063) 128aa long hypothetical
            protein [Aeropyrum pernix]
            Length = 128

Frame  2 hits (HSPs):               _______________                       
                        __________________________________________________
Database sequence:     |                   |                  |           | 128
                       0                  50                100

  Plus Strand HSPs:

 Score = 68 (23.9 bits), Expect = 8.3, P = 1.0
 Identities = 16/37 (43%), Positives = 24/37 (64%), Frame = +2

Query:    71 FPN-PVGCSS-WIAPSGSITQGLLPWPTSSTMRNVLVS 178
             FP+ PVG S+ W  P  ++  GL+PW  S T+  +L+S
Sbjct:    33 FPSSPVGTSTTWNPPMAALA-GLVPWAESGTIITLLLS 69


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.96

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.332   0.145   0.499  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.329   0.138   0.465  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.338   0.146   0.478  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.334   0.144   0.472  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.368   0.165   0.639  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.358   0.163   0.664  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      110       110       10.  69 3  12 22  0.12    32
                                                    29  0.12    34
   +2      0      110       110       10.  69 3  12 22  0.12    32
                                                    29  0.12    34
   +1      0      111       111       10.  70 3  12 22  0.12    32
                                                    29  0.12    34
   -1      0      111       111       10.  70 3  12 22  0.12    32
                                                    29  0.12    34
   -2      0      110       110       10.  69 3  12 22  0.12    32
                                                    29  0.12    34
   -3      0      110       110       10.  69 3  12 22  0.12    32
                                                    29  0.12    34


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  7
  No. of states in DFA:  588 (58 KB)
  Total size of DFA:  167 KB (192 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  121.55u 1.25s 122.80t  Elapsed: 00:00:22
  Total cpu time:  121.58u 1.26s 122.84t  Elapsed: 00:00:22
  Start:  Thu Jan 17 10:31:48 2002   End:  Thu Jan 17 10:32:10 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000