NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 FINAL #1; REVISION #1 MICE Facility: Southern Research Institute Chemical CAS #: 7220-79-3 Lock Date: 09/05/03 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All Page 1 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + M + + + + + + + + + + + + + + + + A + + + + A| Cyst | X X | |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + A| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + A| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + A + + + + A| Edema | 3 | |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + A + + + + A| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + + A + + + + A| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + + A + + + + A| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis, Focal | 2 | Clear Cell Focus | X | Congestion | 3 | Eosinophilic Focus | | Fibrosis, Focal | | Hematopoietic Cell Proliferation | | Hemorrhage | 3 | Hepatodiaphragmatic Nodule | 2 | Infiltration Cellular, Mixed Cell | 1 2 2 2 1 1 1 | Mixed Cell Focus | X | Bile Duct, Cyst | | Hepatocyte, Necrosis, Focal | 2| Hepatocyte, Vacuolization Cytoplasmic| 3 3 2 2 | Kupffer Cell, Pigmentation | 1 | |__________________________________________________________________________| Mesentery | + + + + + + + + + | Artery, Inflammation, Chronic, Focal | 3 | Fat, Necrosis, Focal | 3 1 2 3 1 3 2 3 3 | __________________________________________|__________________________________________________________________________| Page 2 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + A| Acinus, Atrophy, Focal | 4 | Duct, Cyst | X | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Diverticulum | | Inflammation, Focal | | Ulcer | | Epithelium, Hyperplasia | | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + A| Cyst | | Epithelium, Cytoplasmic Alteration, | | Focal | 2 | Epithelium, Hyperplasia | 2 | |__________________________________________________________________________| Tooth | + | Dysplasia | 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Cardiomyopathy | | Thrombosis | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | 1 3 | Cytoplasmic Alteration, Focal | | Degeneration, Cystic | | Infiltration Cellular, Mixed Cell | | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 3 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + A| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + M + + + + + + + + + + + + + + + M| Cyst | | |__________________________________________________________________________| Pituitary Gland | + + + M + + + + + + + + + + + + M + + + + + + + +| Angiectasis | 1 | Pars Distalis, Cytoplasmic | | Alteration, Focal | 1 | Pars Distalis, Hyperplasia, Focal | 1 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + I| Cyst | X | Inflammation, Chronic, Focal | 2 | Follicle, Degeneration, Cystic, Focal| 2 2 1 3 2 2 | Follicular Cell, Hyperplasia, Focal | 1 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | + | Pelvic, Fibrosis | 3 | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + M + + I + + + + + + + + + + + +| |__________________________________________________________________________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + M| Angiectasis | 3 4 | Cyst | X X X X X X | Thrombosis | 4 4 4 | Bilateral, Cyst | X | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Inflammation, Suppurative | | Endometrium, Hyperplasia, Cystic | 3 3 3 3 3 3 2 3 3 3 3 1 3 3 3 3 3 3 3 3 3 3 2| |__________________________________________________________________________| Vagina | + | __________________________________________|__________________________________________________________________________| Page 4 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 4 3 | |__________________________________________________________________________| Lymph Node | + +| Iliac, Angiectasis | | Iliac, Hyperplasia, Lymphoid | | Mediastinal, Hyperplasia, Lymphoid | 3 | Renal, Hyperplasia, Lymphoid | 2| |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + I + + + + + + + + + + + + + + + I +| Hemorrhage | 3 | Hyperplasia, Lymphoid | 2 2 2 2 2 2 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + I + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | 3 | Hyperplasia, Lymphoid | 2 2 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 2 2 3 3 3 3 3 3 3 3 3| Hyperplasia, Lymphoid | 3 2 2 2 3 2 3 2 | |__________________________________________________________________________| Thymus | + + I + + + + + + + + + + + I + + + + + I + + + +| Hyperplasia, Lymphoid | 4 3 2 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 1 2 1 2 | Vertebra, Hypertrophy, Focal | | |__________________________________________________________________________| Skeletal Muscle | | __________________________________________|__________________________________________________________________________| Page 5 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression, Focal | | Artery, Meninges, Inflammation, | | Chronic | 2 | |__________________________________________________________________________| Peripheral Nerve | + | |__________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 3 | Foreign Body | 3 | Hemorrhage | 3 | Hyperplasia, Histiocytic | | Infiltration Cellular, | | Polymorphonuclear | | Infiltration Cellular, Mixed Cell | | Inflammation | 2 | Metaplasia, Focal, Osseous | 1| Alveolar Epithelium, Hyperplasia, | | Focal | 1 2 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + A + + + + A| Anterior Chamber, Inflammation | | Cornea, Inflammation | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | | __________________________________________|__________________________________________________________________________| Page 6 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 6| 7| 7| 7| 7| 5| 7| 7| 1| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 5| 3| 3| 3| 3| 3| 3| 3| 5| 3| 3| 8| 0| 3| 3| 3| 3| 1| 3| 3| 3| 3| 2| | 2| 2| 0| 2| 2| 8| 1| 7| 7| 7| 0| 6| 6| 5| 4| 6| 6| 6| 6| 6| 2| 2| 2| 2| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + A| Hyperplasia, Lymphoid | | Metaplasia, Focal, Osseous | 3 1 | Nephropathy | 1 2 1 1 | Renal Tubule, Accumulation, Hyaline | | Droplet | 3 | Renal Tubule, Cyst | X | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + I + + + + + + + + + + + + + + +| Inflammation | 2 | Transitional Epithelium, Hyperplasia | 2 | __________________________________________|__________________________________________________________________________ Page 7 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + A + A + + + + + + M + + + M A| 42 | Cyst | | 2 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + A + + + + +| 48 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + A + + + + +| 48 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + A + + + + + + + A + A + + + + + + A + + + A +| 43 | Edema | | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + A + + + + + + + A + A + + + + + + A + + + A +| 43 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + A + + + + + + + A + A + + + + + + A + + + A +| 43 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + A + + + + + + + A + A + + + + + + A + + + A +| 43 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis, Focal | 2 | 2 2.0| Clear Cell Focus | X X | 3 | Congestion | | 1 3.0| Eosinophilic Focus | X | 1 | Fibrosis, Focal | 1 | 1 1.0| Hematopoietic Cell Proliferation | 1 1 1 2 | 4 1.3| Hemorrhage | | 1 3.0| Hepatodiaphragmatic Nodule | | 1 2.0| Infiltration Cellular, Mixed Cell | 2 1 2 1 | 11 1.5| Mixed Cell Focus | X | 2 | Bile Duct, Cyst | X | 1 | Hepatocyte, Necrosis, Focal | 1 2 | 3 1.7| Hepatocyte, Vacuolization Cytoplasmic| 3 3 2 3 2 2 3 4| 12 2.7| Kupffer Cell, Pigmentation | | 1 1.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 8 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Mesentery | + + + + + + + + +| 18 | Artery, Inflammation, Chronic, Focal | | 1 3.0| Fat, Necrosis, Focal | 3 3 3 3 1 1 3| 16 2.4| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + A + + + + + + A + + + + A| 46 | Acinus, Atrophy, Focal | | 1 4.0| Duct, Cyst | X | 2 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Diverticulum | 1 | 1 1.0| Inflammation, Focal | 1 | 1 1.0| Ulcer | 2 2 | 2 2.0| Epithelium, Hyperplasia | 3 1 | 2 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + A + + + + + + + A + A + + + + + + A + + + + +| 45 | Cyst | X | 1 | Epithelium, Cytoplasmic Alteration, | | | Focal | | 1 2.0| Epithelium, Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Tooth | + | 2 | Dysplasia | 1 | 2 1.5| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cardiomyopathy | 1 | 1 1.0| Thrombosis | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 9 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Accessory Adrenal Cortical Nodule | | 2 2.0| Cytoplasmic Alteration, Focal | 1 | 1 1.0| Degeneration, Cystic | 1 | 1 1.0| Infiltration Cellular, Mixed Cell | 2 | 1 2.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + A + + + + + + A + + + + A| 46 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Cyst | X | 1 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + I + + + M + + + + + + + +| 46 | Angiectasis | 1 | 2 1.0| Pars Distalis, Cytoplasmic | | | Alteration, Focal | | 1 1.0| Pars Distalis, Hyperplasia, Focal | | 1 1.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + A + A + + + + + + + + + + + A| 46 | Cyst | | 1 | Inflammation, Chronic, Focal | | 1 2.0| Follicle, Degeneration, Cystic, Focal| 1 1 2 2 2 2 1 1 2 2 2 | 17 1.8| Follicular Cell, Hyperplasia, Focal | 1 1 3 | 5 1.6| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + + | 3 | Pelvic, Fibrosis | | 1 3.0| _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 10 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Ovary | + + + + + + + + + + I + + + + + + + + + + + + + A| 47 | Angiectasis | 3 | 3 3.3| Cyst | X X X X X | 11 | Thrombosis | | 3 4.0| Bilateral, Cyst | | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| Inflammation, Suppurative | 3 | 1 3.0| Endometrium, Hyperplasia, Cystic | 3 3 3 3 1 3 2 3 2 3 1 3 3 3 3 3 3 3 3 3 3 3 3 2 3| 48 2.8| |__________________________________________________________________________|____________| Vagina | | 1 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 3 2 3 2 3 3| 10 2.7| |__________________________________________________________________________|____________| Lymph Node | + + + + + | 7 | Iliac, Angiectasis | 3 | 1 3.0| Iliac, Hyperplasia, Lymphoid | 2 | 1 2.0| Mediastinal, Hyperplasia, Lymphoid | 3 | 2 3.0| Renal, Hyperplasia, Lymphoid | | 1 2.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Hemorrhage | | 1 3.0| Hyperplasia, Lymphoid | 2 2 2 | 9 2.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + M + M + + + + + + + + + + + + + + + + + + I| 46 | Hemorrhage | | 1 3.0| Hyperplasia, Lymphoid | 2 3 2 3 | 6 2.3| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + A + + + + + + A + + + + A| 47 | Hematopoietic Cell Proliferation | 3 3 3 3 2 2 3 2 2 3 | 23 2.7| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 11 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Hyperplasia, Lymphoid | 2 2 3 3 3 2 3 3 2 | 17 2.5| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + I + M + + + + + + + + + + + I A| 43 | Hyperplasia, Lymphoid | 2 2 2 2 | 8 2.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | 2 | 5 1.6| Vertebra, Hypertrophy, Focal | 2 1 2 | 3 1.7| |__________________________________________________________________________|____________| Skeletal Muscle | + | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Compression, Focal | 3 | 1 3.0| Artery, Meninges, Inflammation, | | | Chronic | | 1 2.0| |__________________________________________________________________________|____________| Peripheral Nerve | | 1 | |__________________________________________________________________________|____________| Spinal Cord | | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Congestion | | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 12 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| RESPIRATORY SYSTEM - cont | | | Foreign Body | | 1 3.0| Hemorrhage | | 1 3.0| Hyperplasia, Histiocytic | 1 | 1 1.0| Infiltration Cellular, | | | Polymorphonuclear | 3| 1 3.0| Infiltration Cellular, Mixed Cell | 3 | 1 3.0| Inflammation | | 1 2.0| Metaplasia, Focal, Osseous | | 1 1.0| Alveolar Epithelium, Hyperplasia, | | | Focal | | 2 1.5| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + I + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + A + + + + + + + A + + + + + + + + A + + + A A| 43 | Anterior Chamber, Inflammation | 3 | 1 3.0| Cornea, Inflammation | 3 | 1 3.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + A + + + + + + + + + + + +| 49 | Epithelium, Hyperplasia | 2 1| 2 1.5| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + A| 48 | Hyperplasia, Lymphoid | 2 | 1 2.0| Metaplasia, Focal, Osseous | | 2 2.0| Nephropathy | 1 2 | 6 1.3| Renal Tubule, Accumulation, Hyaline | | | Droplet | 3 | 2 3.0| Renal Tubule, Cyst | | 1 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 13 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 6| 6| 7| 7| 6| 6| 7| 5| 7| 7| 7| 6| 7| 7| 6| 6| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 1| 3| 3| 3| 1| 3| 3| 5| 0| 3| 6| 3| 3| 3| 6| 3| 3| 7| 2| 3| 3| 6| 2| | | 1| 7| 8| 7| 7| 8| 7| 7| 1| 5| 4| 6| 8| 6| 6| 7| 3| 7| 7| 3| 7| 6| 6| 6| 9| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | Urinary Bladder | + + + + + + + + + + A + A + + + + + + A + + + I +| 45 | Inflammation | | 1 2.0| Transitional Epithelium, Hyperplasia | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 14 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | 3 | Inflammation | 3 | Perforation | 3 | |__________________________________________________________________________| Gallbladder | + + + + + + + + + + + + + + + + + + + A + + A + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A A + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + A + + + + + +| Artery, Inflammation, Chronic, Focal | 3 | |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + A A + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + A A + + A + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + A A + + A + +| Epithelium, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + A A + + A + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | | Clear Cell Focus | X X | Congestion | | Eosinophilic Focus | X | Hematopoietic Cell Proliferation | 2 | Infiltration Cellular, Mixed Cell | 1 2 1 1 1| Mixed Cell Focus | X | Hepatocyte, Necrosis, Focal | 2 1 2 | Hepatocyte, Vacuolization Cytoplasmic| 2 2 1 3 2 3 2| |__________________________________________________________________________| Mesentery | + + + + + + + | Infiltration Cellular, Lymphoid | 2 | Artery, Inflammation, Chronic, Focal | 3 | Fat, Necrosis, Focal | 3 3 1 3 3 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 15 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Pancreas | + + + + + + + + + + + + + + + I + + + A + + A + +| Acinus, Atrophy, Focal | 4 | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + A + + A + +| |__________________________________________________________________________| Tooth | + + + + +| Dysplasia | 1 1 1 1 1| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Blood Vessel | | Aneurysm | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | 1 2 | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + I + + + A + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + M + + + + + + + + + + + + + + + + + + +| Cyst | | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 1 | Pars Distalis, Cytoplasmic | | Alteration, Focal | 2 1 | Pars Distalis, Hyperplasia, Focal | | __________________________________________|__________________________________________________________________________| Page 16 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation, Chronic, Focal | 2 | C-Cell, Hyperplasia | | Follicle, Degeneration, Cystic, Focal| 2 1 1 1 2 2 1 2 | Follicular Cell, Hyperplasia, Focal | 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Peritoneum | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Cystic | | |__________________________________________________________________________| Ovary | + + I + + + + + + + + + + + + + + + I + + + + + +| Angiectasis | 3 | Cyst | X X | Interstitial Cell, Hyperplasia | 3 | |__________________________________________________________________________| Oviduct | + | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | 3 | Hemorrhage | | Inflammation, Chronic | | Endometrium, Hyperplasia, Cystic | 3 3 3 3 3 3 3 3 3 2 3 3 3 3 3 2 3 1 3 3 1 2 2| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 | |__________________________________________________________________________| Lymph Node | + + + | Pancreatic, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 17 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Hyperplasia, Lymphoid | 4 2 2 3 3 3 2 2 3 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Ectasia | | Hemorrhage | | Hyperplasia, Lymphoid | 2 3 3 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + A + + A + +| Angiectasis, Focal | 3 | Congestion | 3 | Fibrosis, Focal | 3 | Hematopoietic Cell Proliferation | 2 3 3 2 2 3 2 3 | Hyperplasia, Lymphoid | 2 2 2 2 3 3 3 | |__________________________________________________________________________| Thymus | + + + + + + + + + + I + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 2 2 3 2 2| Inflammation | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 1 1 | Vertebra, Hypertrophy, Focal | 1 | |__________________________________________________________________________| Skeletal Muscle | + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 18 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | Spinal Cord | + | Demyelination, Focal | 3 | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | | Mediastinum, Foreign Body | X | Mediastinum, Inflammation | 3 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 2 2 | Nasolacrimal Duct, Inflammation | | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + A + +| Artery, Retrobulbar, Inflammation, | | Chronic, Focal | 2 | Cornea, Inflammation | 3 | Cornea, Necrosis, Focal | 3 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 1 | |__________________________________________________________________________| Lacrimal Gland | + | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | | Metaplasia, Focal, Osseous | | Nephropathy | 2 1 1 | Capsule, Fibrosis, Focal | 2 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + A + + A + +| __________________________________________|__________________________________________________________________________| Page 19 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 5| 7| 7| 7| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 6| 5| 7| 7| 6| 5| 7| DAY ON TEST | 3| 8| 3| 3| 3| 3| 0| 3| 3| 3| 3| 3| 3| 3| 3| 8| 3| 3| 7| 8| 3| 3| 1| 2| 3| | 2| 9| 2| 2| 2| 7| 3| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 3| 5| 3| 3| 6| 1| 3| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| URINARY SYSTEM - cont | | Hyperplasia, Lymphoid | | __________________________________________|__________________________________________________________________________ Page 20 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Foreign Body | | 1 3.0| Inflammation | | 1 3.0| Perforation | | 1 3.0| |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + A + + M + + + + + + + A + + + +| 45 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + A + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + A + + + + + + + + + + + + + + +| 48 | Artery, Inflammation, Chronic, Focal | | 1 3.0| |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + A + + + + + + + + + + A + + + +| 46 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + M + + + + + + + + + + A + + + +| 45 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + A + + + + + + + + + + A + + + +| 45 | Epithelium, Hyperplasia, Focal | | 1 2.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + M + + + + + + + A + + + + + + + + + + A + + + +| 44 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Basophilic Focus | X | 1 | Clear Cell Focus | X | 3 | Congestion | 2 3| 2 2.5| Eosinophilic Focus | | 1 | Hematopoietic Cell Proliferation | | 1 2.0| Infiltration Cellular, Mixed Cell | 1 1 1 1 1 1 2 | 12 1.2| Mixed Cell Focus | | 1 | Hepatocyte, Necrosis, Focal | | 3 1.7| Hepatocyte, Vacuolization Cytoplasmic| 2 1 2 2 | 11 2.0| |__________________________________________________________________________|____________| Mesentery | + + + + + + + | 14 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 21 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Infiltration Cellular, Lymphoid | | 1 2.0| Artery, Inflammation, Chronic, Focal | | 1 3.0| Fat, Necrosis, Focal | 3 3 2 3 3 3 3 | 12 2.8| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + A + + + +| 46 | Acinus, Atrophy, Focal | | 1 4.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 2 2 | 2 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + A + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Tooth | + + + + + | 10 | Dysplasia | 2 1 2 1 1 | 10 1.2| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Blood Vessel | + | 1 | Aneurysm | 4 | 1 4.0| |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Accessory Adrenal Cortical Nodule | | 2 1.5| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + A + + + +| 47 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 22 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Parathyroid Gland | + + + + + + + + + + + M + + + + + + + + + + + + +| 48 | Cyst | X | 1 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + M + + + + + + + + + + + + + + +| 49 | Angiectasis | | 1 1.0| Pars Distalis, Cytoplasmic | | | Alteration, Focal | 1 2 | 4 1.5| Pars Distalis, Hyperplasia, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation, Chronic, Focal | | 1 2.0| C-Cell, Hyperplasia | 2 | 1 2.0| Follicle, Degeneration, Cystic, Focal| 1 1 1 2 1 1 3 | 15 1.5| Follicular Cell, Hyperplasia, Focal | 2 | 2 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Peritoneum | | 1 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Degeneration, Cystic | 2 | 1 2.0| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Angiectasis | 3 | 2 3.0| Cyst | X X X X X X X X X X| 12 | Interstitial Cell, Hyperplasia | | 1 3.0| |__________________________________________________________________________|____________| Oviduct | | 1 | |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | | 1 3.0| Hemorrhage | 3 | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 23 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Inflammation, Chronic | 3 | 1 3.0| Endometrium, Hyperplasia, Cystic | 3 3 1 3 3 3 3 3 3 2 3 3 3 3 2 3 3 3 2 3 3 3 | 45 2.7| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 2 2 | 4 2.0| |__________________________________________________________________________|____________| Lymph Node | + + | 5 | Pancreatic, Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + I + + + + +| 49 | Hyperplasia, Lymphoid | 2 3 | 11 2.6| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + A + + + +| 49 | Ectasia | 3 | 1 3.0| Hemorrhage | 3 3 | 2 3.0| Hyperplasia, Lymphoid | 2 2 2 2 | 7 2.3| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + A + + + +| 47 | Angiectasis, Focal | | 1 3.0| Congestion | 3 | 2 3.0| Fibrosis, Focal | | 1 3.0| Hematopoietic Cell Proliferation | 2 3 2 2 2 3 3 3 2 2 3 2 3| 21 2.5| Hyperplasia, Lymphoid | 2 2 3 2 2 2 2 2 | 15 2.3| |__________________________________________________________________________|____________| Thymus | + I + + + I + + + + + + + + + + + + + + + + + + +| 47 | Hyperplasia, Lymphoid | 2 2 2 2 2 2 2| 13 2.2| Inflammation | | 1 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 24 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| INTEGUMENTARY SYSTEM - cont | | | Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | 2 1 2 | 5 1.4| Vertebra, Hypertrophy, Focal | 2 | 2 1.5| |__________________________________________________________________________|____________| Skeletal Muscle | + +| 4 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | + | 2 | |__________________________________________________________________________|____________| Spinal Cord | + | 2 | Demyelination, Focal | | 1 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemorrhage | 2 | 1 2.0| Mediastinum, Foreign Body | | 1 | Mediastinum, Inflammation | | 1 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | | 3 2.0| Nasolacrimal Duct, Inflammation | 2 | 1 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 25 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 8| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | | 2| 8| 2| 2| 2| 2| 2| 2| 2| 3| 7| 7| 7| 7| 7| 2| 2| 2| 2| 2| 0| 2| 2| 2| 2| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 2| 3| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Eye | + + + + + + + + + A + + + + + + + + + + + + + + +| 48 | Artery, Retrobulbar, Inflammation, | | | Chronic, Focal | | 1 2.0| Cornea, Inflammation | | 1 3.0| Cornea, Necrosis, Focal | | 1 3.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | | 1 1.0| |__________________________________________________________________________|____________| Lacrimal Gland | | 1 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infarct | 3 | 1 3.0| Metaplasia, Focal, Osseous | 1 | 1 1.0| Nephropathy | 1 2 | 5 1.4| Capsule, Fibrosis, Focal | | 1 2.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + A + + + +| 47 | Hyperplasia, Lymphoid | 3 | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 26 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + A + A + + + + + + + + + + + + + + + + +| Cyst | X | |__________________________________________________________________________| Intestine Large, Colon | + + + + + A + A + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + A + A + + + + + + + + + + + + + + + + +| Lymphoid Tissue, Hyperplasia, | | Lymphoid | | |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + A + A + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia, Focal | | |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + A + A + + + + + + + + + + + + + + + + +| Lymphoid Tissue, Hyperplasia | | |__________________________________________________________________________| Intestine Small, Ileum | + + + + + A + A + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X | Congestion | 1 | Eosinophilic Focus | X | Hematopoietic Cell Proliferation | 2 1 1 | Infarct | 3 | Infiltration Cellular, Mixed Cell | 2 1 1 1 2 2 1 1 1 | Hepatocyte, Necrosis, Focal | | Hepatocyte, Vacuolization Cytoplasmic| 2 3 2 2 | Kupffer Cell, Pigmentation | 1 | |__________________________________________________________________________| Mesentery | + + + + + + + | Hemorrhage | 3 | Artery, Inflammation, Chronic, Focal | | Fat, Necrosis, Focal | 1 3 3 3 1 2 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 27 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Pancreas | + + + + + M + + + + + + + + + + + + + + + + + + +| Acinus, Cytoplasmic Alteration | 1 | Duct, Cyst | X X | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Diverticulum | 1 | Inflammation, Focal | 3 | Ulcer | 2 2 | Epithelium, Hyperplasia | 3 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Tooth | + | Dysplasia | 1 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mixed Cell | 3 | Artery, Inflammation, Chronic | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Hyperplasia, Focal | 2 | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + M + + + + +| Hyperplasia, Focal | | |__________________________________________________________________________| Islets, Pancreatic | + + + + + M + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + M + +| Cyst | X | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + M + + + + + + + + + M + + +| __________________________________________|__________________________________________________________________________| Page 28 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Cyst | X | Pars Distalis, Cytoplasmic | | Alteration, Focal | | Pars Distalis, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X| Inflammation, Chronic, Focal | | Follicle, Degeneration, Cystic, Focal| 2 2 2 1 1 1 2 2 1 1 2 1 | Follicular Cell, Hyperplasia, Focal | 1 3 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | None | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + I + + + + + + + + + + + + + + + + +| Degeneration, Cystic | 2 | |__________________________________________________________________________| Ovary | M + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X X X X X| Hemorrhage | 3 | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Endometrium, Hyperplasia, Cystic | 3 3 3 3 1 3 3 3 3 3 2 3 3 3 3 3 3 3 3 1 3 3 3 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 3 2 3 2 2 | |__________________________________________________________________________| Lymph Node | + + + + + | Iliac, Hyperplasia, Lymphoid | | Pancreatic, Hyperplasia, Lymphoid | | Renal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + M + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 29 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Hyperplasia, Lymphoid | 4 2 2 2 2 | Hyperplasia, Mast Cell | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Ectasia | 3 3 | Hemorrhage | 3 3 | Hyperplasia, Histiocytic | 3 | Hyperplasia, Lymphoid | 4 3 2 2 2 | |__________________________________________________________________________| Spleen | + + + + + + + A + + + + + + + + + + + + + + + + +| Fibrosis, Focal | 3 | Hematopoietic Cell Proliferation | 3 2 2 2 2 2 3 3 3 2 2 3 | Hyperplasia, Lymphoid | 2 2 2 3 2 3 3 2 2| |__________________________________________________________________________| Thymus | + + I + + + + I + + + + I + + + + + + + + + I + +| Hyperplasia, Lymphoid | 4 2 4 2 2 2 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 2 2 2| Vertebra, Hypertrophy, Focal | | |__________________________________________________________________________| Skeletal Muscle | + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | | |__________________________________________________________________________| Spinal Cord | | __________________________________________|__________________________________________________________________________| Page 30 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 5| 7| 2| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 1| 3| 6| 3| 8| 3| 3| 3| 3| 1| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| | 3| 3| 3| 6| 3| 8| 3| 3| 3| 3| 3| 3| 6| 3| 0| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | 4 | Inflammation | 4 | Alveolar Epithelium, Hyperplasia, | | Focal | 1 | Mediastinum, Foreign Body | X | Mediastinum, Inflammation | 3 | Serosa, Fibrosis | 3 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 2 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + A + A + + + + + + + + + + + + + + + + +| Cornea, Inflammation | 2 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + A + + + + + + + + + + + + + + + + +| Infarct | 2 3 | Metaplasia, Focal, Osseous | 2 | Nephropathy | 1 1 1 1 2 1 | Renal Tubule, Cyst | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 31 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | Cyst | | 1 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | Lymphoid Tissue, Hyperplasia, | | | Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | Epithelium, Hyperplasia, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | Lymphoid Tissue, Hyperplasia | 4 | 1 4.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Clear Cell Focus | X X X | 4 | Congestion | | 1 1.0| Eosinophilic Focus | X X | 3 | Hematopoietic Cell Proliferation | 2 1 3 | 6 1.7| Infarct | | 1 3.0| Infiltration Cellular, Mixed Cell | 2 2 2 | 12 1.5| Hepatocyte, Necrosis, Focal | 2 | 1 2.0| Hepatocyte, Vacuolization Cytoplasmic| 2 3 2 2| 8 2.3| Kupffer Cell, Pigmentation | | 1 1.0| |__________________________________________________________________________|____________| Mesentery | + + + + + + + + | 15 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 32 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Hemorrhage | | 1 3.0| Artery, Inflammation, Chronic, Focal | 3 | 1 3.0| Fat, Necrosis, Focal | 3 3 1 3 3 3 1 | 13 2.3| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Acinus, Cytoplasmic Alteration | | 1 1.0| Duct, Cyst | | 2 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Diverticulum | | 1 1.0| Inflammation, Focal | | 1 3.0| Ulcer | 2 | 3 2.0| Epithelium, Hyperplasia | 2 | 2 2.5| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Tooth | + + | 3 | Dysplasia | 1 2 | 3 1.3| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mixed Cell | | 1 3.0| Artery, Inflammation, Chronic | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 | 1 2.0| Hyperplasia, Focal | | 1 2.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 33 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Hyperplasia, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Parathyroid Gland | M + + M + + M + + + + + + + + + + + + + + + + + +| 46 | Cyst | | 1 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Cyst | | 1 | Pars Distalis, Cytoplasmic | | | Alteration, Focal | 2 | 1 2.0| Pars Distalis, Hyperplasia, Focal | | 1 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | Cyst | | 1 | Inflammation, Chronic, Focal | 2 | 1 2.0| Follicle, Degeneration, Cystic, Focal| 2 1 2 2 2 1 2 2 2 1 2 3 2 1 1 1| 28 1.6| Follicular Cell, Hyperplasia, Focal | 2 2 | 4 2.0| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | None | | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + + I + + + + + + + + + + I I +| 46 | Degeneration, Cystic | | 1 2.0| |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Cyst | X X X X X X X X X X X | 16 | Hemorrhage | | 1 3.0| |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 3 | 1 3.0| Endometrium, Hyperplasia, Cystic | 3 3 3 3 3 3 3 3 3 3 3 3 1 3 1 3 2 2 3 3 3 3 3 3 3| 49 2.8| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 34 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + I + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia | 2 2 3 | 9 2.3| |__________________________________________________________________________|____________| Lymph Node | + + + + +| 10 | Iliac, Hyperplasia, Lymphoid | 3 2 | 2 2.5| Pancreatic, Hyperplasia, Lymphoid | 3| 1 3.0| Renal, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + M + + + + + + +| 48 | Hyperplasia, Lymphoid | 1 2 2 2 2 3 3 | 12 2.3| Hyperplasia, Mast Cell | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + I + + + + + + + + + + +| 49 | Ectasia | | 2 3.0| Hemorrhage | | 2 3.0| Hyperplasia, Histiocytic | | 1 3.0| Hyperplasia, Lymphoid | 3 3 2 2 3 | 10 2.6| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Fibrosis, Focal | | 1 3.0| Hematopoietic Cell Proliferation | 3 2 3 2 2 2 2 2 3 2 3 2 3 2 3 3 2 2 2| 31 2.4| Hyperplasia, Lymphoid | 2 2 2 2 3 2 2 2| 17 2.2| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + I + I + + + + + + + + + + +| 44 | Hyperplasia, Lymphoid | 2 2 2 3 2 | 11 2.5| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 35 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | 1 | 4 1.8| Vertebra, Hypertrophy, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Skeletal Muscle | + + | 3 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | + | 1 | |__________________________________________________________________________|____________| Spinal Cord | + | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Foreign Body | | 1 4.0| Inflammation | | 1 4.0| Alveolar Epithelium, Hyperplasia, | | | Focal | | 1 1.0| Mediastinum, Foreign Body | | 1 | Mediastinum, Inflammation | | 1 3.0| Serosa, Fibrosis | | 1 3.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 2 2 2 2 2 | 7 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + A + + + + + + + + + + +| 47 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 36 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| | DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 0| 3| 3| 2| 3| 3| 3| 3| 3| | | 2| 2| 2| 2| 2| 3| 3| 3| 3| 3| 7| 7| 5| 6| 7| 6| 5| 6| 6| 7| 3| 3| 3| 3| 3| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Cornea, Inflammation | | 1 2.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Infarct | | 2 2.5| Metaplasia, Focal, Osseous | | 1 2.0| Nephropathy | 1 1 1| 9 1.1| Renal Tubule, Cyst | X X X| 3 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + A + + + + + + + + + + +| 49 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 37 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + + + + + + + + A + + + + + A + + + + + A + + + +| Cyst | | |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + M + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + + + + + + + + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | + + + + + + + + A + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + + + + + + + + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + + + + + + + + + + + + A + + + + + A + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X X | Congestion | 3 | Eosinophilic Focus | X | Hematopoietic Cell Proliferation | 1 1 | Hemorrhage | 1 | Infiltration Cellular, Mixed Cell | 1 1 1 1 1 2 1 1 1 1 | Mixed Cell Focus | | Hepatocyte, Vacuolization Cytoplasmic| 3 3 3 3 3 2 2 2 2 | |__________________________________________________________________________| Mesentery | + + + + + | Hemorrhage | | Fat, Necrosis, Focal | 1 3 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy, Diffuse | 4 | Acinus, Atrophy, Focal | 1 | Duct, Cyst | X | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 38 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Diverticulum | 1 1 | Ulcer | | Epithelium, Hyperplasia | | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + + + + A + + + + + + + + + +| Cyst | | |__________________________________________________________________________| Tooth | + + | Dysplasia | 1 2 | _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Cardiomyopathy | | Mineralization, Focal | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Parathyroid Gland | + + + + + M + + + + + + + + + + + + + + + + + + +| Cyst | | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + M + + + + + + + +| Cyst | | Pars Distalis, Cytoplasmic | | Alteration, Focal | 2 | Pars Distalis, Hyperplasia, Focal | | |__________________________________________________________________________| Thyroid Gland | + + + + + M + + + + + + + + + + + + + + A + + + +| C-Cell, Hyperplasia | 2 | __________________________________________|__________________________________________________________________________| Page 39 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Follicle, Degeneration, Cystic, Focal| 2 2 2 2 2 2 2 1 1 2 1 2 1 1 1 | Follicular Cell, Hyperplasia, Focal | 1 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | + | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Clitoral Gland | + + + + + + + + + + + + + + + + + M + + + + + + +| |__________________________________________________________________________| Ovary | + + + + + + + + + + + I + + + + + + + + + + + + +| Angiectasis | 3 2 | Cyst | X X X X| Hemorrhage | | Pigmentation, Focal | 3 | Thrombosis | 3 | |__________________________________________________________________________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| Endometrium, Hyperplasia, Cystic | 3 3 3 3 3 3 3 3 1 3 3 4 2 3 2 3 3 3 3 3 3 3 3 3 3| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 2 | |__________________________________________________________________________| Lymph Node | + + | Iliac, Hyperplasia, Lymphoid | 3 | Mediastinal, Hyperplasia, Lymphoid | 3 | Mediastinal, Inflammation | | Pancreatic, Hyperplasia, Lymphoid | | Renal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + I + + + + + + + + + + + +| Hyperplasia, Lymphoid | 2 2 2 2 2 2 2 2 2 2| |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | 3 | __________________________________________|__________________________________________________________________________| Page 40 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Hyperplasia, Lymphoid | 2 2 2 3 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 3 2 2 3 2 2 3 2 2 2 2 3 2 3 3 3 2 2 2 2| Hyperplasia, Lymphoid | 3 2 2 2 3 2 | |__________________________________________________________________________| Thymus | + + + + + + + + + + + + + + + + + + + + M + + + +| Hyperplasia, Lymphoid | 2 2 2 2 2 2| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Edema | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 2 2 | Vertebra, Hypertrophy, Focal | | |__________________________________________________________________________| Skeletal Muscle | + + + | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression, Focal | 3| |__________________________________________________________________________| Peripheral Nerve | | |__________________________________________________________________________| Spinal Cord | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Larynx | M | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 41 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| 5| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| | 3| 3| 3| 3| 3| 2| 2| 2| 2| 2| 2| 2| 2| 2| 9| 6| 6| 6| 6| 6| 9| 6| 6| 6| 6| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Hyperplasia, Histiocytic | | Hyperplasia, Lymphoid | | Alveolar Epithelium, Hyperplasia, | | Focal | | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 1 2 2 | Nasolacrimal Duct, Hyperplasia, Focal| 2 | |__________________________________________________________________________| Trachea | + + + + + M + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | Cataract | | Cornea, Inflammation | 3 | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 3 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | | Infarct | 2 | Nephropathy | 1 1 1 | Bilateral, Infarct | | Renal Tubule, Accumulation, Hyaline | | Droplet | 3 | |__________________________________________________________________________| Ureter | + | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + I + + + +| Transitional Epithelium, Hyperplasia | 2 | __________________________________________|__________________________________________________________________________ Page 42 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + + + + + A + + + A + + M + + + + + + + + + + +| 44 | Cyst | X X | 2 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + A + + +| 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + A + + +| 46 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + A + + + A + + A + + + + A + + + + + +| 44 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + A + + + A + + A + + + + A + + A + + +| 42 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + A + + + A + + A + + + + A + + A + + +| 43 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + A + + + A + + A + + + + A + + A + + +| 43 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Basophilic Focus | X X | 3 | Clear Cell Focus | | 2 | Congestion | | 1 3.0| Eosinophilic Focus | | 1 | Hematopoietic Cell Proliferation | | 2 1.0| Hemorrhage | | 1 1.0| Infiltration Cellular, Mixed Cell | 1 1 1 1 1 | 15 1.1| Mixed Cell Focus | X | 1 | Hepatocyte, Vacuolization Cytoplasmic| 2 2 2 2 2 1 | 15 2.3| |__________________________________________________________________________|____________| Mesentery | + + + + + + + + | 13 | Hemorrhage | 2 | 1 2.0| Fat, Necrosis, Focal | 3 3 | 4 2.5| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + A + + +| 49 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 43 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Acinus, Atrophy, Diffuse | | 1 4.0| Acinus, Atrophy, Focal | | 1 1.0| Duct, Cyst | X | 2 | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + M + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + A + + + + + + + + + + + + + +| 49 | Diverticulum | 1 | 3 1.0| Ulcer | 1 | 1 1.0| Epithelium, Hyperplasia | 2 | 1 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + A + + + A + + + + + + + + + + A + + +| 46 | Cyst | X | 1 | |__________________________________________________________________________|____________| Tooth | + | 3 | Dysplasia | 1 | 3 1.3| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cardiomyopathy | 1 | 1 1.0| Mineralization, Focal | 1 | 1 1.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + A + + + + + + + + + + A + + +| 48 | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Cyst | X | 1 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 44 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + M + + M| 47 | Cyst | X | 1 | Pars Distalis, Cytoplasmic | | | Alteration, Focal | 2 | 2 2.0| Pars Distalis, Hyperplasia, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + A + + + + + + + + + + + + + +| 47 | C-Cell, Hyperplasia | | 1 2.0| Follicle, Degeneration, Cystic, Focal| 2 2 2 2 1 2 2 2 | 23 1.7| Follicular Cell, Hyperplasia, Focal | 1 2 2 | 5 1.4| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + + | 3 | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Clitoral Gland | + + + + + + + + + + I + + + + + + + + + + + + + +| 48 | |__________________________________________________________________________|____________| Ovary | + + + + + + + + + + + + + + + + + + + + + A + + +| 48 | Angiectasis | | 2 2.5| Cyst | X X X X X X X X X | 13 | Hemorrhage | 3 | 1 3.0| Pigmentation, Focal | | 1 3.0| Thrombosis | 3 4 | 3 3.3| |__________________________________________________________________________|____________| Uterus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Endometrium, Hyperplasia, Cystic | 3 2 3 3 3 3 2 3 2 3 3 3 1 3 3 3 3 1 3 2 3 3 3| 48 2.8| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 3 | 4 2.5| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 45 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | Lymph Node | + + + + + + + + | 10 | Iliac, Hyperplasia, Lymphoid | 2 | 2 2.5| Mediastinal, Hyperplasia, Lymphoid | | 1 3.0| Mediastinal, Inflammation | 3 | 1 3.0| Pancreatic, Hyperplasia, Lymphoid | 3 | 1 3.0| Renal, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + M + + + + + + M + + + + + + I + +| 46 | Hyperplasia, Lymphoid | 2 2 3 2 3 3 | 16 2.2| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + I + + + + I + + + + + + + + + + +| 48 | Hemorrhage | | 1 3.0| Hyperplasia, Lymphoid | 2 3 2 2| 8 2.3| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 2 3 2 2 2 2 3 2 3 3 3 3 2 2 2 2 2 2| 40 2.3| Hyperplasia, Lymphoid | 2 2 3 3 3 | 11 2.5| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia, Lymphoid | 2 2 2 3 2 2 2 2 | 14 2.1| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Edema | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperostosis | 2 2 3 | 5 2.2| Vertebra, Hypertrophy, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 46 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | Skeletal Muscle | | 3 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + M + + +| 49 | Compression, Focal | | 1 3.0| |__________________________________________________________________________|____________| Peripheral Nerve | + | 1 | |__________________________________________________________________________|____________| Spinal Cord | + | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Larynx | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Histiocytic | 3 | 1 3.0| Hyperplasia, Lymphoid | 3 | 1 3.0| Alveolar Epithelium, Hyperplasia, | | | Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 2 2 2 2 2 2 2 | 11 1.9| Nasolacrimal Duct, Hyperplasia, Focal| | 1 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + A + + + A + + + + + + + + + + A + + +| 47 | Atrophy | 4 | 1 4.0| Cataract | 3 | 1 3.0| Cornea, Inflammation | | 1 3.0| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 47 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 4| 7| 6| 5| 7| 7| 7| 7| 4| 7| 7| 7| 7| 7| 7| | DAY ON TEST | 2| 2| 2| 2| 2| 3| 4| 3| 3| 3| 7| 3| 8| 2| 3| 3| 3| 3| 4| 3| 3| 3| 3| 3| 3| | | 9| 9| 9| 9| 9| 2| 9| 2| 2| 2| 1| 3| 0| 8| 3| 7| 7| 7| 5| 7| 7| 2| 7| 7| 7| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE FEMALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 2 | 3 2.3| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemorrhage | 3 | 1 3.0| Infarct | 2 | 2 2.0| Nephropathy | 1 2 | 5 1.2| Bilateral, Infarct | 4 | 1 4.0| Renal Tubule, Accumulation, Hyaline | | | Droplet | 2 | 2 2.5| |__________________________________________________________________________|____________| Ureter | | 1 | |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + A + + + + + + + + + + + + + +| 48 | Transitional Epithelium, Hyperplasia | | 1 2.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 48 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7| DAY ON TEST | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2| | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | | |__________________________________________________________________________| Gallbladder | A + + + + + + + + + + + + + + + + + A + A + + + M| |__________________________________________________________________________| Intestine Large, Colon | A + + + + + + + + + + + + + + + A + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | A + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | A + + + + + + + + + + + + + + + A + + + + + + + +| |__________________________________________________________________________| Intestine Small, Duodenum | A + + + + + + + + + + + M + + + A + + + I + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | A + + + + + + + + + + + + + + + A + + + + + + + +| Inflammation | 2 | Lymphoid Tissue, Hyperplasia | | |__________________________________________________________________________| Intestine Small, Ileum | A + + + + + + + + + + + + + + + A + + I A + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X X X X X X X X X X| Fibrosis, Focal | | Hematopoietic Cell Proliferation | | Infarct | 4 | Infiltration Cellular, Mixed Cell | 1 | Mixed Cell Focus | X X | Hepatocyte, Necrosis, Focal | 2 | Hepatocyte, Vacuolization Cytoplasmic| 2 2 2 2 2 2 1 2 2 3 2 3 3| |__________________________________________________________________________| Mesentery | + + | Fat, Necrosis, Focal | 4 3 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + A + + + + + + + +| Acinus, Atrophy, Diffuse | | Acinus, Atrophy, Focal | | Acinus, Cytoplasmic Alteration | | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 49 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7| DAY ON TEST | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2| | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 2 2 | |__________________________________________________________________________| Stomach, Glandular | A + + + + + + + + + + + + + + + A + + + + + + + +| Erosion | 2 2 | Ulcer | | |__________________________________________________________________________| Tooth | + + + + + + + + + + + + + + + + + + + +| Dysplasia | 2 4 4 4 4 4 1 3 4 2 4 3 1 4 3 4 2 2 2 3| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mixed Cell | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + I + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | 1 | Cytoplasmic Alteration, Focal | 2 1 | Degeneration, Cystic | | Hypertrophy | | Inflammation | | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + I + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 1 | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 2 2 2 2 2 2 3 2 2 2| |__________________________________________________________________________| Parathyroid Gland | + M + + + M + + + + + + + + + + + + + + + + M M +| |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X X | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 50 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7| DAY ON TEST | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2| | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Follicle, Degeneration, Cystic, Focal| 2 2 1 1 1 | Follicular Cell, Hyperplasia, Focal | 3 1 1 2 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | | Pelvic, Inflammation | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Cystic | 3 3 3 3 3 | Inflammation, Chronic | 3 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 | Epithelium, Hyperplasia | 3 | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Dilatation | 3 3 | |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Angiectasis | | Atrophy | | Thrombosis | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 | |__________________________________________________________________________| Lymph Node | + | Inguinal, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | A + + M + M + + + + + + + + M + + + + + + + + + +| Hyperplasia, Lymphoid | 3 | __________________________________________|__________________________________________________________________________| Page 51 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7| DAY ON TEST | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2| | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + + A + + + + + + + +| Hyperplasia, Lymphoid | | |__________________________________________________________________________| Spleen | A + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 3 3 3 | Hyperplasia, Lymphoid | 2 2 | Hyperplasia, Mast Cell | | Lymphoid Follicle, Necrosis | | |__________________________________________________________________________| Thymus | I M + + + I + + + + + I I + + M I + I + + + + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst Epithelial Inclusion | | Subcutaneous Tissue, Edema | 2 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + | |__________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 2 | Hyperplasia, Histiocytic | 2 | Inflammation | | __________________________________________|__________________________________________________________________________| Page 52 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 6| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 6| 7| 7| 7| 7| 4| 6| 6| 5| 7| 7| 5| 7| DAY ON TEST | 9| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 2| 3| 3| 2| 7| 0| 6| 4| 2| 2| 4| 2| | 4| 0| 1| 0| 0| 0| 0| 6| 0| 2| 0| 1| 0| 9| 1| 0| 3| 8| 5| 5| 8| 9| 9| 4| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0 MG/KG | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Alveolar Epithelium, Hyperplasia, | | Focal | 2 2 | Mediastinum, Foreign Body | X | Mediastinum, Inflammation, | | Suppurative | 3 | Mediastinum, Thrombosis | 4 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 | Nasolacrimal Duct, Inflammation | 2 2 | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | A + + + + + + + + + + + + + + + A + + + + + + + +| Cornea, Inflammation | 2 | Cornea, Necrosis, Focal | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | | Epithelium, Hyperplasia | | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | A + + + + + + + + + + + + + + + + + + + + + + + +| Hydronephrosis | | Metaplasia, Focal, Osseous | 1 2| Nephropathy | 2 1 1 1 1 2 2 2 2 2 2 3 2 1 2 2 1 2 1 1| Renal Tubule, Cyst | X X | Renal Tubule, Hyperplasia, Focal | 1 2 1 | |__________________________________________________________________________| Urethra | | Inflammation, Chronic | | |__________________________________________________________________________| Urinary Bladder | A + + + + + + + + + + + + + + + + + + + + + + + +| Transitional Epithelium, Hyperplasia | | __________________________________________|__________________________________________________________________________ Page 53 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 3 | 1 3.0| |__________________________________________________________________________|____________| Gallbladder | A + + + + + M + + + + + + + + M I + + + + A A + +| 40 | |__________________________________________________________________________|____________| Intestine Large, Colon | A + + + + + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | A + + + + + + + + + + + + + + + + + + + + + + + +| 48 | |__________________________________________________________________________|____________| Intestine Large, Cecum | A + + + + + + + + + + + + + + + A + + + + A + + +| 45 | |__________________________________________________________________________|____________| Intestine Small, Duodenum | A + + + + + + + + + + + + + + + A + + + + A M + +| 42 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | A + + + + + + + + + + + + + + + A + + + + A + + +| 45 | Inflammation | | 1 2.0| Lymphoid Tissue, Hyperplasia | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | A + + + + + + + + + + + + + + + A + + + + A + + +| 43 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Clear Cell Focus | X X X X X X X X X X| 20 | Fibrosis, Focal | 3 | 1 3.0| Hematopoietic Cell Proliferation | 1 | 1 1.0| Infarct | | 1 4.0| Infiltration Cellular, Mixed Cell | 2 2 | 3 1.7| Mixed Cell Focus | X | 3 | Hepatocyte, Necrosis, Focal | 3 | 2 2.5| Hepatocyte, Vacuolization Cytoplasmic| 2 2 2 2 3 2 3 3 2 3 2 2 3 2 3 3| 29 2.3| |__________________________________________________________________________|____________| Mesentery | + + + | 5 | Fat, Necrosis, Focal | 3 2 | 4 3.0| |__________________________________________________________________________|____________| Pancreas | A + + + + + + + + + + + + + + + + + + + + + + + +| 48 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 54 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Acinus, Atrophy, Diffuse | 4 | 1 4.0| Acinus, Atrophy, Focal | 2 | 1 2.0| Acinus, Cytoplasmic Alteration | 1 | 1 1.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 2 | 3 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + A + +| 47 | Erosion | | 2 2.0| Ulcer | 3 | 1 3.0| |__________________________________________________________________________|____________| Tooth | + + + + + + + + + + + + + + + + + + + + + + +| 43 | Dysplasia | 1 2 1 4 2 2 2 4 2 4 3 2 2 2 1 4 4 2 3 3 2 2 3| 43 2.7| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mixed Cell | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + M + + + + + + +| 48 | Accessory Adrenal Cortical Nodule | | 1 1.0| Cytoplasmic Alteration, Focal | 1 2 | 4 1.5| Degeneration, Cystic | 2 | 1 2.0| Hypertrophy | 1 | 1 1.0| Inflammation | 3 | 1 3.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + M + + + + + + +| 48 | Hyperplasia, Focal | 2 | 2 1.5| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 55 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Hyperplasia | 2 2 2 3 2| 17 2.1| |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 46 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst | X | 3 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Degeneration, Cystic, Focal| 2 | 6 1.5| Follicular Cell, Hyperplasia, Focal | 2 2 2 1 1 2 2 2 1 | 14 1.7| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + | 1 | Pelvic, Inflammation | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Degeneration, Cystic | 3 3 3 3 3 3 | 11 3.0| Inflammation, Chronic | 3 | 2 3.0| |__________________________________________________________________________|____________| Prostate | A + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Inflammation | | 1 2.0| Epithelium, Hyperplasia | | 1 3.0| |__________________________________________________________________________|____________| Seminal Vesicle | A + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Dilatation | | 2 3.0| |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Angiectasis | 2 | 1 2.0| Atrophy | 2 | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 56 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Thrombosis | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node | + + + + | 5 | Inguinal, Hyperplasia, Lymphoid | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | M + + + + I + + + + + + + + + + M + + + + + + + +| 43 | Hyperplasia, Lymphoid | 3 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | A + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Hyperplasia, Lymphoid | 4 3 | 2 3.5| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hematopoietic Cell Proliferation | 2 2 3 3 3 2 3 2 3 2 | 14 2.6| Hyperplasia, Lymphoid | 2 2 2 2 2 | 7 2.0| Hyperplasia, Mast Cell | 2 | 1 2.0| Lymphoid Follicle, Necrosis | 3 | 1 3.0| |__________________________________________________________________________|____________| Thymus | I + + + + + + + + I + + I I + + + + + + + + + + +| 38 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst Epithelial Inclusion | X | 1 | Subcutaneous Tissue, Edema | | 1 2.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 57 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | M + | 2 | |__________________________________________________________________________|____________| Spinal Cord | + + + | 4 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Congestion | | 1 2.0| Hyperplasia, Histiocytic | | 1 2.0| Inflammation | 2 | 1 2.0| Alveolar Epithelium, Hyperplasia, | | | Focal | 1 1 | 4 1.5| Mediastinum, Foreign Body | | 1 | Mediastinum, Inflammation, | | | Suppurative | | 1 3.0| Mediastinum, Thrombosis | | 1 4.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | | 1 2.0| Nasolacrimal Duct, Inflammation | 2 | 3 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | A + + + + + + + + + + + + + + + A + + + + A + + +| 45 | Cornea, Inflammation | 2 | 2 2.0| Cornea, Necrosis, Focal | 2 | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 58 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 5| 6| 7| 7| | DAY ON TEST | 2| 0| 3| 3| 3| 2| 7| 3| 3| 2| 3| 2| 3| 2| 3| 2| 3| 3| 2| 2| 2| 5| 0| 3| 3| | | 3| 5| 0| 1| 1| 9| 3| 0| 0| 9| 0| 9| 0| 9| 0| 9| 0| 0| 9| 9| 9| 9| 5| 1| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| A | 0 MG/KG | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 3 | 1 3.0| Epithelium, Hyperplasia | 2 2 | 2 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Hydronephrosis | 3 3 | 2 3.0| Metaplasia, Focal, Osseous | 1 | 3 1.3| Nephropathy | 2 1 2 1 1 1 1 2 1 1 1 1 2 2 2 1 2 1 2 1| 40 1.5| Renal Tubule, Cyst | X X | 4 | Renal Tubule, Hyperplasia, Focal | | 3 1.3| |__________________________________________________________________________|____________| Urethra | + | 1 | Inflammation, Chronic | 3 | 1 3.0| |__________________________________________________________________________|____________| Urinary Bladder | A + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Transitional Epithelium, Hyperplasia | 3 | 1 3.0| __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 59 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3| | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | | Inflammation | | Perforation | | |__________________________________________________________________________| Gallbladder | + A + M + + + + + + + + + + + + + + A + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + A + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + A + + + + + + + + + + + + + + + + A + + + + + +| Edema | 3 | |__________________________________________________________________________| Intestine Small, Duodenum | + A + + + + + + + + + + + + + + + + A + + + + + +| Epithelium, Cyst | | |__________________________________________________________________________| Intestine Small, Jejunum | + A + + + + + + + + + + + + + + + + A + + + + + +| Peyer's Patch, Hyperplasia, Lymphoid | 2 | |__________________________________________________________________________| Intestine Small, Ileum | + A + + + + + + + + + + + + + + + + A + + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Basophilic Focus | X | Clear Cell Focus | X X X X X X X X X X X| Cyst | | Eosinophilic Focus | X | Hematopoietic Cell Proliferation | 3 | Hemorrhage | | Hepatodiaphragmatic Nodule | | Infiltration Cellular, Mixed Cell | 1 | Mixed Cell Focus | X | Thrombosis | 3 | Bile Duct, Hyperplasia | | Hepatocyte, Necrosis, Focal | 3 | Hepatocyte, Vacuolization Cytoplasmic| 3 3 2 3 3 3 2 3 3 3 3 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 60 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3| | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Mesentery | + + + + + + | Inflammation | 3 | Fat, Necrosis, Focal | 2 1 3 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy, Focal | | Acinus, Cytoplasmic Alteration | 2 | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | 2 2 | Epithelium, Hyperplasia | 2 | |__________________________________________________________________________| Stomach, Glandular | + A + + + + + + + + + + + + + + + + A + + + + + +| Erosion | 2 | |__________________________________________________________________________| Tooth | + + + + + + + + + + + + + + + + +| Dysplasia | 4 1 1 1 3 4 2 2 1 2 2 3 2 4 1 2 3| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mixed Cell | 2 | Artery, Inflammation, Chronic | 2 | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Hypertrophy, Focal | | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | 1 | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 1 1 2 2 | |__________________________________________________________________________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 61 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3| | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Cyst | X X | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + M + + + + + + + + + + +| Cyst | | Pars Distalis, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Degeneration, Cystic, Focal| 1 3 2 1 1 1 1 | Follicular Cell, Hyperplasia, Focal | 2 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | + | Abdominal, Fibrosis | 3 | Abdominal, Inflammation | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Cystic | 3 3 3 3 | Inflammation, Chronic | 3 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 3 | Epithelium, Hyperplasia | 3 | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Dilatation | | |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 62 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3| | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Lymph Node | + + | Iliac, Hyperplasia, Lymphoid | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + M + + + + + + + M + + + + + + + + + + + M| Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| Hematopoietic Cell Proliferation | 2 2 4 3 3 2 2 3 | Hyperplasia, Lymphoid | 3 2 2| |__________________________________________________________________________| Thymus | + I + + + + + + + + + + + + + I + + + + + + I + +| Angiectasis | 3 | Hyperplasia, Lymphoid | 3 | Epithelial Cell, Hyperplasia | 3 | _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Edema | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Vertebra, Fracture | | |__________________________________________________________________________| Skeletal Muscle | + | Fibrosis, Focal | 1 | Inflammation | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 63 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 6| 7| 4| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| DAY ON TEST | 3| 9| 3| 5| 3| 3| 3| 2| 3| 3| 3| 3| 3| 3| 4| 3| 3| 3| 1| 2| 3| 2| 3| 7| 3| | 1| 4| 2| 2| 1| 1| 1| 9| 1| 1| 0| 0| 0| 2| 7| 0| 0| 1| 7| 9| 0| 9| 1| 0| 2| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 2.5 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| NERVOUS SYSTEM - cont | | Peripheral Nerve | + | |__________________________________________________________________________| Spinal Cord | + | Hemorrhage | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | | Alveolar Epithelium, Hyperplasia, | | Focal | 2 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 1 | Nasolacrimal Duct, Hyperplasia, Focal| | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Infarct | 2 | Nephropathy | 2 2 1 2 2 1 1 3 1 2 2 1 3 1 2 1 2 1 1 2 3 2| Renal Tubule, Cyst | | Renal Tubule, Hyperplasia, Atypical | | Renal Tubule, Hyperplasia, Focal | 2 | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 64 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Foreign Body | 2 | 1 2.0| Inflammation | 3 | 1 3.0| Perforation | 3 | 1 3.0| |__________________________________________________________________________|____________| Gallbladder | + + + + + + + + + + + + + + + + A + + + A + + A +| 44 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + + + + + + + + + + + + + + + + + A + + A +| 46 | Edema | | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + + + + + + + + + + + + + + + + + A + + A +| 46 | Epithelium, Cyst | X | 1 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + + + + + + + + + + + + + + + A + A + + A +| 45 | Peyer's Patch, Hyperplasia, Lymphoid | | 1 2.0| |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + + + + + + + + + + + + + + + I + A + + A +| 45 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Basophilic Focus | | 1 | Clear Cell Focus | X X X X X X X X | 19 | Cyst | X | 1 | Eosinophilic Focus | X | 2 | Hematopoietic Cell Proliferation | 2 | 2 2.5| Hemorrhage | 3 | 1 3.0| Hepatodiaphragmatic Nodule | 2 | 1 2.0| Infiltration Cellular, Mixed Cell | | 1 1.0| Mixed Cell Focus | | 1 | Thrombosis | 3 | 2 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 65 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Bile Duct, Hyperplasia | 1 | 1 1.0| Hepatocyte, Necrosis, Focal | 3 | 2 3.0| Hepatocyte, Vacuolization Cytoplasmic| 3 2 3 3 1 2 3 2| 19 2.6| |__________________________________________________________________________|____________| Mesentery | + + + + + | 11 | Inflammation | | 1 3.0| Fat, Necrosis, Focal | 3 1 3 3 | 7 2.3| |__________________________________________________________________________|____________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Acinus, Atrophy, Focal | 1 | 1 1.0| Acinus, Cytoplasmic Alteration | | 1 2.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Ulcer | 2 | 3 2.0| Epithelium, Hyperplasia | 2 | 2 2.0| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + A +| 47 | Erosion | | 1 2.0| |__________________________________________________________________________|____________| Tooth | + + + + + + + + + + + + + + + + + + + +| 37 | Dysplasia | 3 2 4 2 3 1 4 4 2 4 2 2 4 4 2 2 1 3 4 2| 37 2.5| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mixed Cell | | 1 2.0| Artery, Inflammation, Chronic | | 1 2.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hypertrophy, Focal | 3 | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 66 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | | 1 1.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 2 2 2 2 2 2 2 1 2 2 1 2 | 17 1.8| |__________________________________________________________________________|____________| Parathyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst | X | 3 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Cyst | X | 1 | Pars Distalis, Hyperplasia, Focal | | 1 2.0| |__________________________________________________________________________|____________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Follicle, Degeneration, Cystic, Focal| 1 1 1 1 1 | 12 1.3| Follicular Cell, Hyperplasia, Focal | 2 1 1 2 | 5 1.6| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + | 2 | Abdominal, Fibrosis | 3 | 2 3.0| Abdominal, Inflammation | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Degeneration, Cystic | 3 3 3 2 3 3 3 | 11 2.9| Inflammation, Chronic | | 1 3.0| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 67 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| GENITAL SYSTEM - cont | | | Epithelium, Hyperplasia | | 1 3.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Dilatation | 3 3 | 2 3.0| |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 3 | 3 3.0| |__________________________________________________________________________|____________| Lymph Node | + | 3 | Iliac, Hyperplasia, Lymphoid | 2 | 1 2.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + I + + + + + + + +| 46 | Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Lymphoid | 3 | 2 3.0| |__________________________________________________________________________|____________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hematopoietic Cell Proliferation | 3 3 3 2 2 3 3 3 | 16 2.7| Hyperplasia, Lymphoid | 2 2 2| 6 2.2| |__________________________________________________________________________|____________| Thymus | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Angiectasis | | 1 3.0| Hyperplasia, Lymphoid | | 1 3.0| Epithelial Cell, Hyperplasia | | 1 3.0| _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 68 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| INTEGUMENTARY SYSTEM - cont | | | Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Edema | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Vertebra, Fracture | 3 | 1 3.0| |__________________________________________________________________________|____________| Skeletal Muscle | + + + | 4 | Fibrosis, Focal | | 1 1.0| Inflammation | 2 | 1 2.0| _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | + + | 3 | |__________________________________________________________________________|____________| Spinal Cord | + + + | 4 | Hemorrhage | 3 3 | 2 3.0| _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Congestion | 3 | 1 3.0| Alveolar Epithelium, Hyperplasia, | | | Focal | 1 2 | 3 1.7| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 2 1 | 3 1.3| Nasolacrimal Duct, Hyperplasia, Focal| 3 | 1 3.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 69 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 6| 7| 0| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| 7| 6| 7| 6| 7| 7| | DAY ON TEST | 2| 7| 3| 1| 2| 3| 3| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 2| 0| 2| 1| 3| 2| 2| 3| | | 9| 6| 0| 5| 9| 2| 2| 9| 9| 1| 1| 0| 1| 0| 0| 1| 8| 9| 4| 9| 0| 1| 7| 5| 0| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| A | 2.5 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + A +| 49 | |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + A +| 49 | Infarct | | 1 2.0| Nephropathy | 3 1 2 1 2 2 1 1 1 1 1 2 2 2 2 1 2 1 3 2 3 1| 44 1.7| Renal Tubule, Cyst | X X | 2 | Renal Tubule, Hyperplasia, Atypical | 2 | 1 2.0| Renal Tubule, Hyperplasia, Focal | 3 | 2 2.5| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 70 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Gallbladder | + A + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Colon | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + A + + + + + + + + + A + + + + + + + A + + + + +| Edema | | |__________________________________________________________________________| Intestine Small, Duodenum | + M + + + + + + + + + + + + + + + + + A + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + A + + + + + + + + + + + + + + + + + A + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + A + + + + + + + + + A + + + + + + + A + + + + +| |__________________________________________________________________________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X X X X X X X X X X X| Eosinophilic Focus | X X | Infiltration Cellular, Mixed Cell | | Thrombosis | | Hepatocyte, Necrosis, Focal | | Hepatocyte, Vacuolization Cytoplasmic| 2 3 2 3 2 2 3 2 1 2 3 3 2 2 2 3 3 2 3| Hepatocyte, Centrilobular, Necrosis | | |__________________________________________________________________________| Mesentery | + + | Fat, Necrosis, Focal | 1 | |__________________________________________________________________________| Pancreas | + + + + + + + + + + + + + + + + + + + + + + + + +| Acinus, Atrophy, Focal | 2 2 | Acinus, Inflammation | 2 | Duct, Cyst | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 71 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Epithelium, Hyperplasia | 1 2 | |__________________________________________________________________________| Stomach, Glandular | + + + + + + + + + + + A + + + + + + + A + + + + +| Erosion | 1 | |__________________________________________________________________________| Tooth | + + + + + + + + + + + + + + + + +| Dysplasia | 3 1 2 2 2 2 2 3 4 2 4 3 1 1 2 2 3| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Infiltration Cellular, Mixed Cell | 2 | Mineralization, Focal | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| Accessory Adrenal Cortical Nodule | | Cytoplasmic Alteration, Focal | 2 | Degeneration, Cystic | 2 | |__________________________________________________________________________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | |__________________________________________________________________________| Islets, Pancreatic | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 2| |__________________________________________________________________________| Parathyroid Gland | + + + + + + + I + + + + + + + + + + + + + + + + +| Cyst | X X | |__________________________________________________________________________| Pituitary Gland | I + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X | |__________________________________________________________________________| Thyroid Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Follicle, Degeneration, Cystic, Focal| 2 1 2 1 2 1 1 2 | Follicular Cell, Hyperplasia, Focal | 3 2 1 2 1| _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | __________________________________________|__________________________________________________________________________| Page 72 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| GENERAL BODY SYSTEM - cont | | |__________________________________________________________________________| Peritoneum | + | |__________________________________________________________________________| Tissue NOS | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + M + + + + + + + + + + + + +| |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Degeneration, Cystic | 3 3 3 3 3 3 3 3| Inflammation, Chronic | 3 | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 2 | |__________________________________________________________________________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | | |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 3 | |__________________________________________________________________________| Lymph Node | + + | Mediastinal, Hemorrhage | | |__________________________________________________________________________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + I + + + + + +| Hemorrhage | 3 | Hyperplasia, Lymphoid | 3 3 | |__________________________________________________________________________| Spleen | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 73 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | Atrophy | 3 | Hematopoietic Cell Proliferation | 3 3 3 2 3 2 2 4 3 3 2 2 | Hyperplasia, Lymphoid | 2 | Lymphoid Follicle, Necrosis | 2 | |__________________________________________________________________________| Thymus | + + + + + + + M + + M M + I + + M + + + + I I + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Subcutaneous Tissue, Edema | 3 | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| Compression, Focal | | |__________________________________________________________________________| Peripheral Nerve | | |__________________________________________________________________________| Spinal Cord | | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Hemorrhage | 2 | Hyperplasia, Histiocytic | 4 3 | Thrombosis | 2 | Alveolar Epithelium, Hyperplasia, | | Focal | 1 | |__________________________________________________________________________| __________________________________________|__________________________________________________________________________| Page 74 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 2| 7| 6| 7| 6| 7| 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| DAY ON TEST | 3| 6| 2| 2| 2| 0| 3| 3| 3| 3| 3| 9| 3| 3| 3| 3| 3| 3| 3| 2| 3| 3| 3| 3| 3| | 1| 1| 9| 7| 9| 5| 1| 0| 0| 0| 1| 5| 1| 1| 0| 0| 1| 0| 1| 7| 0| 1| 1| 0| 1| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 12.5 | 0| 0| 0| 0| 0| 0| 0| 0| 0| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| 2| 2| 2| 2| 2| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | 2 | Nasolacrimal Duct, Inflammation | | |__________________________________________________________________________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| Atrophy | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Epithelium, Hyperplasia | 2 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Lymphoid | | Metaplasia, Focal, Osseous | 1 1 | Mineralization, Focal | | Nephropathy | 1 1 2 2 2 2 1 2 1 1 1 3 2 2 1 2 1 1 1 1 2 2| Artery, Inflammation, Chronic | | Renal Tubule, Accumulation, Hyaline | | Droplet | | Renal Tubule, Cyst | X X | Renal Tubule, Hyperplasia, Focal | 1 | Renal Tubule, Pigmentation | | |__________________________________________________________________________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 75 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Gallbladder | + + A + + + + + + + I + + + M + + + + + M + + + +| 45 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + A + + + + + + + + + + + + + + + + + + + + + +| 46 | Edema | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + A + + + I + + + + + + + + + + + + + + + + + +| 46 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + A + + + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + A + + + + + + M + + + + + + + + + + M + + + +| 44 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Clear Cell Focus | X X X X X X X X X X X | 22 | Eosinophilic Focus | | 2 | Infiltration Cellular, Mixed Cell | 1 1 | 2 1.0| Thrombosis | 3 | 1 3.0| Hepatocyte, Necrosis, Focal | 3 3 | 2 3.0| Hepatocyte, Vacuolization Cytoplasmic| 3 2 3 2 3 2 3 2 3 3 3 3 2 | 32 2.5| Hepatocyte, Centrilobular, Necrosis | 3 | 1 3.0| |__________________________________________________________________________|____________| Mesentery | + + + | 5 | Fat, Necrosis, Focal | 3 3 3 | 4 2.5| |__________________________________________________________________________|____________| Pancreas | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | Acinus, Atrophy, Focal | | 2 2.0| Acinus, Inflammation | | 1 2.0| Duct, Cyst | X | 1 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 76 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 2 | 3 1.7| |__________________________________________________________________________|____________| Stomach, Glandular | + + A + + + + + + + + + + + + + + + + + + + + + +| 47 | Erosion | 3 | 2 2.0| |__________________________________________________________________________|____________| Tooth | + + + + + + + + + + + + + + + + | 33 | Dysplasia | 2 4 4 2 1 1 3 4 2 2 3 2 2 3 2 4 | 33 2.4| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Infiltration Cellular, Mixed Cell | | 1 2.0| Mineralization, Focal | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Accessory Adrenal Cortical Nodule | 1 | 1 1.0| Cytoplasmic Alteration, Focal | 1 2 2 1 | 5 1.6| Degeneration, Cystic | | 1 2.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | Hyperplasia | 1 2 1 1 | 7 1.6| |__________________________________________________________________________|____________| Parathyroid Gland | + + + M + + + + + + + + + + + + + + + + + + + + +| 48 | Cyst | X | 3 | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 77 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Cyst | | 1 | |__________________________________________________________________________|____________| Thyroid Gland | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | Follicle, Degeneration, Cystic, Focal| 2 1 1 | 11 1.5| Follicular Cell, Hyperplasia, Focal | 3 1 1 2 1 2 | 11 1.7| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Peritoneum | | 1 | |__________________________________________________________________________|____________| Tissue NOS | M | | _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Degeneration, Cystic | 3 3 3 3 3 3 3 2 | 16 2.9| Inflammation, Chronic | 3 | 2 3.0| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | | 1 2.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 1 | 1 1.0| |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Atrophy | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 | 3 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 78 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | |__________________________________________________________________________|____________| Lymph Node | + | 3 | Mediastinal, Hemorrhage | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + M + + + + + + + + + + + + + + + + + M + + + +| 48 | Hemorrhage | 3 | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + A + + + + + + + + + + + + + + + + + + + + + +| 48 | Hemorrhage | 3 3 | 3 3.0| Hyperplasia, Lymphoid | | 2 3.0| |__________________________________________________________________________|____________| Spleen | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | Atrophy | | 1 3.0| Hematopoietic Cell Proliferation | 3 3 3 3 3 3 2 3 3 3 3 3 3 | 25 2.8| Hyperplasia, Lymphoid | 2 | 2 2.0| Lymphoid Follicle, Necrosis | | 1 2.0| |__________________________________________________________________________|____________| Thymus | + + + + + I + + + A + + + + + + + + + + M + + + +| 40 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Subcutaneous Tissue, Edema | 3 | 2 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Skeletal Muscle | + | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 79 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| NERVOUS SYSTEM - cont | | | Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Compression, Focal | 3 | 1 3.0| |__________________________________________________________________________|____________| Peripheral Nerve | + | 1 | |__________________________________________________________________________|____________| Spinal Cord | + | 1 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hemorrhage | | 1 2.0| Hyperplasia, Histiocytic | 2 | 3 3.0| Thrombosis | | 1 2.0| Alveolar Epithelium, Hyperplasia, | | | Focal | 1 | 2 1.0| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 2 3 | 3 2.3| Nasolacrimal Duct, Inflammation | 2 | 1 2.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + A + + + + + + + + + + + + + + + + + + + + + +| 49 | Atrophy | 4 | 1 4.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Epithelium, Hyperplasia | 2 | 2 2.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia, Lymphoid | 3 | 1 3.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 80 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 6| 7| 7| 7| 7| 7| 7| 6| 6| 7| 7| 7| 7| 7| 7| 7| 7| 6| 6| 6| 7| 7| 6| | DAY ON TEST | 2| 3| 7| 3| 2| 3| 3| 3| 3| 0| 9| 3| 3| 3| 3| 2| 3| 3| 2| 9| 8| 4| 3| 3| 3| | | 9| 0| 3| 1| 9| 1| 1| 0| 1| 5| 0| 1| 1| 1| 1| 9| 1| 0| 9| 6| 0| 5| 0| 1| 8| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| A | 12.5 | 2| 2| 2| 2| 3| 3| 3| 3| 3| 3| 3| 3| 3| 3| 4| 4| 4| 4| 4| 4| 4| 4| 4| 4| 5| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| URINARY SYSTEM - cont | | | Metaplasia, Focal, Osseous | 1 | 3 1.0| Mineralization, Focal | 2 | 1 2.0| Nephropathy | 2 1 2 2 2 1 3 2 2 2 1 1 1 2 1 2 1 3 2 1 2 4| 44 1.7| Artery, Inflammation, Chronic | 2 | 1 2.0| Renal Tubule, Accumulation, Hyaline | | | Droplet | 2 | 1 2.0| Renal Tubule, Cyst | X | 3 | Renal Tubule, Hyperplasia, Focal | | 1 1.0| Renal Tubule, Pigmentation | 2 | 1 2.0| |__________________________________________________________________________|____________| Urinary Bladder | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 81 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| DAY ON TEST | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2| | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM | | |__________________________________________________________________________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| Foreign Body | | Inflammation | 3 | Perforation | 3 | |__________________________________________________________________________| Gallbladder | + + A A + + + + + + + + A + + + + + + + + + M + +| |__________________________________________________________________________| Intestine Large, Colon | + + + A + + + + + + + + A + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Rectum | + + + A + + + + + + + + A + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Large, Cecum | + + + A + + + + + + + + A + + + + + + + + + + + +| Inflammation, Chronic, Focal | | |__________________________________________________________________________| Intestine Small, Duodenum | + + + A + + + + + + + + A + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Jejunum | + + + A + + + + + + + + A + + + + + + + + + + + +| |__________________________________________________________________________| Intestine Small, Ileum | + + + A + + + + + + + + A + + + + + + + + + + + +| |__________________________________________________________________________| Liver | + + + A + + + + + + + + + + + + + + + + + + + + +| Clear Cell Focus | X X X X X X X X X | Congestion | | Cyst | X | Eosinophilic Focus | X | Hepatodiaphragmatic Nodule | | Infiltration Cellular, Mixed Cell | 1 2 1| Thrombosis | | Hepatocyte, Necrosis, Focal | 1 | Hepatocyte, Syncytial Alteration, | | Focal | 1 | Hepatocyte, Vacuolization Cytoplasmic| 2 2 2 2 2 3 2 2 3 2 3 2| |__________________________________________________________________________| Mesentery | + + + | Inflammation | | |__________________________________________________________________________| Pancreas | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 82 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| DAY ON TEST | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2| | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ALIMENTARY SYSTEM - cont | | Acinus, Atrophy, Focal | | |__________________________________________________________________________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Stomach, Forestomach | + + + A + + + + + + + + + + + + + + + + + + + + +| Ulcer | | Epithelium, Hyperplasia | | |__________________________________________________________________________| Stomach, Glandular | + + + A + + + + + + + + + + + + + + + + + + + + +| Erosion | | |__________________________________________________________________________| Tooth | + + + + + + + + + + + + + + + + + + + +| Dysplasia | 2 2 3 2 2 3 3 3 1 2 3 3 3 4 3 1 2 2 2 4| _____________________________________________________________________________________________________________________| CARDIOVASCULAR SYSTEM | | |__________________________________________________________________________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| Mineralization, Focal | | _____________________________________________________________________________________________________________________| ENDOCRINE SYSTEM | | |__________________________________________________________________________| Adrenal Cortex | + + + A + + + + + + + + + + + + + + + + + + + + +| Cytoplasmic Alteration, Focal | 1 | Hyperplasia, Focal | | |__________________________________________________________________________| Adrenal Medulla | + + + A + + + + + + + + + + + + + + + + + + + + +| Hyperplasia, Focal | | |__________________________________________________________________________| Islets, Pancreatic | + + + A + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 2 2 2 2 3 2 2 | |__________________________________________________________________________| Parathyroid Gland | + + + M + + + + + + + + + + + + + + + M + + I + +| Cyst | X | |__________________________________________________________________________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | | |__________________________________________________________________________| Thyroid Gland | + + + M + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________| Page 83 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| DAY ON TEST | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2| | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| ENDOCRINE SYSTEM - cont | | Follicle, Degeneration, Cystic, Focal| 1 1 1 1 1 | Follicular Cell, Hyperplasia, Focal | 1 2 1 | _____________________________________________________________________________________________________________________| GENERAL BODY SYSTEM | | |__________________________________________________________________________| Tissue NOS | | Foreign Body | | _____________________________________________________________________________________________________________________| GENITAL SYSTEM | | |__________________________________________________________________________| Coagulating Gland | + | |__________________________________________________________________________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Preputial Gland | + + + + + + + + + + + + + + + + + I + + + + + + I| Degeneration, Cystic | 3 3 3 3 | Inflammation, Chronic | | |__________________________________________________________________________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| Inflammation | | |__________________________________________________________________________| Seminal Vesicle | + + + A + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| HEMATOPOIETIC SYSTEM | | |__________________________________________________________________________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | 3 | |__________________________________________________________________________| Lymph Node | + + + + + | Mediastinal, Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Lymph Node, Mandibular | + + M + + + + + + + + + + + + + + + + M + + + + M| Hyperplasia, Lymphoid | 3 | |__________________________________________________________________________| Lymph Node, Mesenteric | + + + + + + + + + + + + + + + + + + + + + + I + +| Hematopoietic Cell Proliferation | 3 | __________________________________________|__________________________________________________________________________| Page 84 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| DAY ON TEST | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2| | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| HEMATOPOIETIC SYSTEM - cont | | |__________________________________________________________________________| Spleen | + + + A + + + + + + + + + + + + + + + + + + + + +| Fibrosis, Focal | | Hematopoietic Cell Proliferation | 3 2 2 3 3 2 2 3 3 2 2 2 3 3 2 4 3 3 | Hyperplasia, Lymphoid | 2 2 | |__________________________________________________________________________| Thymus | + + + A + + + + + I + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| INTEGUMENTARY SYSTEM | | |__________________________________________________________________________| Mammary Gland | M M M M M M M M M M M M + M M M M M M M M M M M M| |__________________________________________________________________________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| Ulcer | 3 | Pinna, Inflammation, Focal | | Subcutaneous Tissue, Edema | 3 | Subcutaneous Tissue, Inflammation, | | Focal, Suppurative | | _____________________________________________________________________________________________________________________| MUSCULOSKELETAL SYSTEM | | |__________________________________________________________________________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperostosis | 1 2 | |__________________________________________________________________________| Skeletal Muscle | | _____________________________________________________________________________________________________________________| NERVOUS SYSTEM | | |__________________________________________________________________________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| |__________________________________________________________________________| Peripheral Nerve | + | |__________________________________________________________________________| Spinal Cord | + | _____________________________________________________________________________________________________________________| RESPIRATORY SYSTEM | | |__________________________________________________________________________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| Congestion | 3 | __________________________________________|__________________________________________________________________________| Page 85 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 _____________________________________________________________________________________________________________________ | 7| 7| 6| 6| 6| 7| 7| 7| 7| 7| 7| 7| 5| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| 7| DAY ON TEST | 2| 3| 1| 8| 4| 3| 2| 2| 2| 2| 2| 2| 0| 3| 3| 3| 2| 2| 3| 2| 3| 3| 9| 3| 2| | 9| 1| 0| 7| 1| 0| 9| 9| 9| 9| 9| 9| 6| 1| 0| 0| 9| 9| 1| 9| 1| 0| 0| 0| 9| __________________________________________|__________________________________________________________________________| | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 25 | 5| 5| 5| 5| 5| 5| 5| 5| 5| 6| 6| 6| 6| 6| 6| 6| 6| 6| 6| 7| 7| 7| 7| 7| 7| MG/KG | 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| __________________________________________|__________________________________________________________________________| RESPIRATORY SYSTEM - cont | | Hemorrhage | 3 | Hyperplasia, Histiocytic | 1 | Alveolar Epithelium, Hyperplasia, | | Focal | 1 1 | |__________________________________________________________________________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| Hyperplasia | | Inflammation | 2 1 2 2 | Nasolacrimal Duct, Hyperplasia, Focal| | Nasolacrimal Duct, Inflammation | | |__________________________________________________________________________| Trachea | + + + M + + + + + + + + + + + + + + + + + + + + +| _____________________________________________________________________________________________________________________| SPECIAL SENSES SYSTEM | | |__________________________________________________________________________| Eye | + + + A I + + + + + + + A + + + + + + + + + + + +| Cataract | | Cornea, Inflammation | | Retrobulbar, Inflammation, Chronic, | | Focal | | |__________________________________________________________________________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| Cyst | X | Inflammation | 1 | _____________________________________________________________________________________________________________________| URINARY SYSTEM | | |__________________________________________________________________________| Kidney | + + + A + + + + + + + + + + + + + + + + + + + + +| Infarct | 2 | Metaplasia, Focal, Osseous | 1 1 | Nephropathy | 1 2 1 1 1 1 1 1 1 1 1 3 3 2 1 2 2 2 1 1| Renal Tubule, Cyst | X | Renal Tubule, Hyperplasia, Focal | | |__________________________________________________________________________| Urinary Bladder | + + + A + + + + + + + + + + + + + + + + + + + + +| __________________________________________|__________________________________________________________________________ Page 86 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Esophagus | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Foreign Body | 3 | 1 3.0| Inflammation | | 1 3.0| Perforation | | 1 3.0| |__________________________________________________________________________|____________| Gallbladder | + + + + A + + + + + + + + + + + + + + + + + + A +| 44 | |__________________________________________________________________________|____________| Intestine Large, Colon | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Large, Rectum | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Large, Cecum | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | Inflammation, Chronic, Focal | 3 | 1 3.0| |__________________________________________________________________________|____________| Intestine Small, Duodenum | + + + + A + + + + + + + + + + + + + + + + + + I +| 46 | |__________________________________________________________________________|____________| Intestine Small, Jejunum | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Intestine Small, Ileum | + + + + A + + + + + + + + + + + + + + + + + + + +| 47 | |__________________________________________________________________________|____________| Liver | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | Clear Cell Focus | X X X X X X X | 16 | Congestion | 3 | 1 3.0| Cyst | | 1 | Eosinophilic Focus | X | 2 | Hepatodiaphragmatic Nodule | X | 1 | Infiltration Cellular, Mixed Cell | 2 1 1| 6 1.3| Thrombosis | 3 | 1 3.0| Hepatocyte, Necrosis, Focal | 1 3 3 | 4 2.0| Hepatocyte, Syncytial Alteration, | | | Focal | | 1 1.0| Hepatocyte, Vacuolization Cytoplasmic| 3 2 3 2 2 2 3 3 1 1 2 | 23 2.2| |__________________________________________________________________________|____________| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 87 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ALIMENTARY SYSTEM - cont | | | Mesentery | + | 4 | Inflammation | 2 | 1 2.0| |__________________________________________________________________________|____________| Pancreas | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Acinus, Atrophy, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Salivary Glands | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Stomach, Forestomach | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Ulcer | 2 | 1 2.0| Epithelium, Hyperplasia | 2 3 | 2 2.5| |__________________________________________________________________________|____________| Stomach, Glandular | + + + + + + + + + + + + + + + + + + + + + + + A +| 48 | Erosion | 2 | 1 2.0| |__________________________________________________________________________|____________| Tooth | + + + + + + + + + + + + + + + | 35 | Dysplasia | 2 4 1 1 4 3 3 2 3 4 4 3 2 3 2 | 35 2.6| _____________________________________________________________________________________________________________________| | CARDIOVASCULAR SYSTEM | | | |__________________________________________________________________________|____________| Heart | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Mineralization, Focal | 1 | 1 1.0| _____________________________________________________________________________________________________________________| | ENDOCRINE SYSTEM | | | |__________________________________________________________________________|____________| Adrenal Cortex | + + + + + + + + + + + + + + + + + + + + I + + + +| 48 | Cytoplasmic Alteration, Focal | 2 2 3 1 | 5 1.8| Hyperplasia, Focal | 2 | 1 2.0| |__________________________________________________________________________|____________| Adrenal Medulla | + + + + + + + + + + + + + + + + + + + + M + + + +| 48 | Hyperplasia, Focal | 1 | 1 1.0| |__________________________________________________________________________|____________| Islets, Pancreatic | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Hyperplasia | 2 2 2 3 2 1 | 13 2.1| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 88 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| ENDOCRINE SYSTEM - cont | | | |__________________________________________________________________________|____________| Parathyroid Gland | + + + + M + + + + + + + + + + + + + + + + + + + +| 46 | Cyst | X | 2 | |__________________________________________________________________________|____________| Pituitary Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst | X X X | 3 | |__________________________________________________________________________|____________| Thyroid Gland | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Follicle, Degeneration, Cystic, Focal| 2 2 | 7 1.3| Follicular Cell, Hyperplasia, Focal | 2 2 2 1 2 2 1 1| 11 1.5| _____________________________________________________________________________________________________________________| | GENERAL BODY SYSTEM | | | |__________________________________________________________________________|____________| Tissue NOS | + | 1 | Foreign Body | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | GENITAL SYSTEM | | | |__________________________________________________________________________|____________| Coagulating Gland | | 1 | |__________________________________________________________________________|____________| Epididymis | + + + + + + + + + + + + + + + + + + + + + + + M +| 49 | |__________________________________________________________________________|____________| Preputial Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 48 | Degeneration, Cystic | 3 3 3 3 3 3 3 | 11 3.0| Inflammation, Chronic | 3 3 3 | 3 3.0| |__________________________________________________________________________|____________| Prostate | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Inflammation | 2 | 1 2.0| |__________________________________________________________________________|____________| Seminal Vesicle | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | |__________________________________________________________________________|____________| Testes | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________| | HEMATOPOIETIC SYSTEM | | | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 89 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| HEMATOPOIETIC SYSTEM - cont | | | |__________________________________________________________________________|____________| Bone Marrow | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node | + | 6 | Mediastinal, Hyperplasia, Lymphoid | | 1 3.0| |__________________________________________________________________________|____________| Lymph Node, Mandibular | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Hyperplasia, Lymphoid | 3 | 2 3.0| |__________________________________________________________________________|____________| Lymph Node, Mesenteric | + + + + M + + + + + + + + + + + + + + + + + + + +| 48 | Hematopoietic Cell Proliferation | | 1 3.0| |__________________________________________________________________________|____________| Spleen | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Fibrosis, Focal | 2 | 1 2.0| Hematopoietic Cell Proliferation | 2 2 2 2 2 2 2 3 3 3 3| 29 2.5| Hyperplasia, Lymphoid | 2 | 3 2.0| |__________________________________________________________________________|____________| Thymus | + I + + A + + + + + + + + + + + + + + + I + + + I| 44 | _____________________________________________________________________________________________________________________| | INTEGUMENTARY SYSTEM | | | |__________________________________________________________________________|____________| Mammary Gland | M M M M M M M M M M M M M M M M M M M M M M M M M| 1 | |__________________________________________________________________________|____________| Skin | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Ulcer | 3| 2 3.0| Pinna, Inflammation, Focal | 2| 1 2.0| Subcutaneous Tissue, Edema | | 1 3.0| Subcutaneous Tissue, Inflammation, | | | Focal, Suppurative | 3 | 1 3.0| _____________________________________________________________________________________________________________________| | MUSCULOSKELETAL SYSTEM | | | |__________________________________________________________________________|____________| Bone | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 90 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| MUSCULOSKELETAL SYSTEM - cont | | | Hyperostosis | | 2 1.5| |__________________________________________________________________________|____________| Skeletal Muscle | + | 1 | _____________________________________________________________________________________________________________________| | NERVOUS SYSTEM | | | |__________________________________________________________________________|____________| Brain | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | |__________________________________________________________________________|____________| Peripheral Nerve | + | 2 | |__________________________________________________________________________|____________| Spinal Cord | + | 2 | _____________________________________________________________________________________________________________________| | RESPIRATORY SYSTEM | | | |__________________________________________________________________________|____________| Lung | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Congestion | | 1 3.0| Hemorrhage | | 1 3.0| Hyperplasia, Histiocytic | 2 | 2 1.5| Alveolar Epithelium, Hyperplasia, | | | Focal | 2 1 | 4 1.3| |__________________________________________________________________________|____________| Nose | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Hyperplasia | 3 | 1 3.0| Inflammation | 2 2 | 6 1.8| Nasolacrimal Duct, Hyperplasia, Focal| 2 | 1 2.0| Nasolacrimal Duct, Inflammation | 1 | 1 1.0| |__________________________________________________________________________|____________| Trachea | + + + + + + + + + + + + + + + + + + + + + + + + +| 49 | _____________________________________________________________________________________________________________________| | SPECIAL SENSES SYSTEM | | | |__________________________________________________________________________|____________| Eye | + + + + + + + + + + + + + + + + + + + + + + + + +| 47 | Cataract | 2 | 1 2.0| Cornea, Inflammation | 2 | 1 2.0| _____________________________________________________________________________________________________________________|____________| * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 91 NTP Experiment-Test: 91005-08 NONNEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Report: PEIRPT09 Study Type: CHRONIC METHYLENE BLUE TRIHYDRATE Date: 06/02/05 Route: GAVAGE Time: 13:15:34 __________________________________________________________________________________________________________________________________ | 7| 7| 7| 7| 6| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 7| 6| 7| | DAY ON TEST | 3| 3| 3| 2| 1| 3| 0| 3| 2| 3| 2| 1| 3| 3| 2| 3| 3| 2| 3| 2| 3| 3| 3| 9| 3| | | 2| 1| 1| 9| 0| 1| 5| 0| 9| 1| 9| 6| 0| 1| 9| 1| 0| 9| 0| 9| 2| 0| 0| 4| 1| | __________________________________________|__________________________________________________________________________| T (*) | | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| O | B6C3F1 MICE MALE | 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| 0| T | ANIMAL ID | 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 1| 2| A | 25 | 7| 7| 7| 7| 8| 8| 8| 8| 8| 8| 8| 8| 8| 8| 9| 9| 9| 9| 9| 9| 9| 9| 9| 9| 0| L | MG/KG | 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| 1| 2| 3| 4| 5| 6| 7| 8| 9| 0| | _____________________________________________________________________________________________________________________|____________| SPECIAL SENSES SYSTEM - cont | | | Retrobulbar, Inflammation, Chronic, | | | Focal | 3 | 1 3.0| |__________________________________________________________________________|____________| Harderian Gland | + + + + + + + + + + + + + + + + + + + + + + + + +| 50 | Cyst | | 1 | Inflammation | | 1 1.0| _____________________________________________________________________________________________________________________| | URINARY SYSTEM | | | |__________________________________________________________________________|____________| Kidney | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | Infarct | | 1 2.0| Metaplasia, Focal, Osseous | 2 1 | 4 1.3| Nephropathy | 1 1 2 1 1 2 2 1 1 3 1 1 1 1 2 1 2 2 1 1 1 2 3| 43 1.5| Renal Tubule, Cyst | X | 2 | Renal Tubule, Hyperplasia, Focal | 2 1 | 2 1.5| |__________________________________________________________________________|____________| Urinary Bladder | + + + + A + + + + + + + + + + + + + + + + + + + +| 48 | __________________________________________________________________________________________________________________________________ * : Total animals with tissue examined microscopically; total animals with lesion and mean severity grade + : Tissue examined microscopically M : Missing tissue 1-4 : Lesion qualified as: X : Lesion present but not qualified A : Autolysis precludes examination 1) Minimal 3) Moderate I : Insufficient tissue BLANK : Not examined 2) Mild 4) Marked Page 92 ------------------------------------------------------------ ---------- END OF REPORT ---------- ------------------------------------------------------------