Keywords: corrinoid, methyltransferases
Copyright © International Union of Crystallography 2005 Isolation, crystallization and preliminary X-ray analysis of a methanol-induced corrinoid protein from Moorella thermoacetica
Correspondence e-mail: larsljd/at/uga.edu, Email: wang/at/bcl1.bmb.uga.edu Received March 1, 2005; Accepted April 4, 2005. | ||||
Abstract A corrinoid protein was induced and overexpressed in methanol-grown cells of the thermophilic anaerobic bacterium Moorella thermoacetica. The protein was purified from cytosolic extracts. After screening for crystallization conditions and optimization, crystals were obtained that diffracted strongly on a rotating-anode X-ray source. A diffraction data set was collected and processed including reflections to 1.9 Å resolution. Reflections were indexed in a primitive orthorhombic cell with unit-cell parameters a = 55.69, b = 62.74, c = 34.54 Å. N-terminal amino-acid sequencing indicates that the crystals contain a C-terminal fragment of the protein. Keywords: corrinoid, methyltransferases | ||||