PELAEZ T, GARCIA-ARIAS V, ALCALA L, FERNANDEZ-CHICO A, BLAZQUEZ A, GUINEA JV, MUNOZ P, BOUZA E; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. M-1222.

Hospital Gregorio Maranon, Madrid, Spain.

BACKGROUND: Trichosporon infections are associated with a wide spectrum of clinical manifestations, ranging from superficial cutaneous involvement in immunocompetent individuals to severe systemic diseases in immunocompromised patients. Epidemiology and antifungal susceptibility data of Trichosporon spp are not widely available. We aim to evaluate the epidemiology and the antifungal susceptibilities of Trichosporon spp in our hospital during a 15-year period METHODS: From 1988 to 2002 121 isolates of Trichosporon spp (97 patients) were recovered. The species of Trichosporon spp identified by ATB ID 32C were: 65 T. asahii, 13 T. mucoides, 11 T. inkin, 2 T. cutaneum and 30 Trichosporon spp. The in vitro activities of amphotericin B (AMB), fluconazole (FZ), itraconazole (IZ), ketoconazole (KZ), voriconazole (VZ) and flucytosine were performed by microdilution method (NCCLS M27-A). MICs were visually determined at 24 and 48 h of incubation at 35degreesC. RESULTS: The rate of isolation of Trichosporon spp during the study period was: 1988-92 (n= 22), 1993-97 (n= 39), 1998-2002 (n= 60). Among the 121 isolates 9 (8 patients) were responsible for fungemia and the remaining 112 were recovered from superficial lesions not implying systemic involvement. The overall MIC[90]s (mg/L) of AMB, FZ, IZ, KZ, VZ and MIC[90]s FC against 56 available Trichosporon spp were: 2, 4, 0.25, 0.25, 0.06 and 32. The MICs (microg/ml) of T. asahii, T. mucoides and T. inkin were respectively: AMB (2/0.5/0.5), FZ (4/2/1), IZ (0.25/0.125/0.06), KZ (0.25/0.03/0.06), VZ (0.06/0.03/0.03) and 5FC (32/64/16). Azole compounds appear to be more active in vitro against Trichosporon compared to AMB. CONCLUSION: A clear increase in the rate of isolation of Trichosporon spp. occurred in the last five years in our institution. Although there are no established breakpoints to define antifungal susceptibility to Trichosporon spp., azoles in general appear to be more active in vitro compared to AMB.

Publication Types:

• Meeting Abstracts

Keywords:

• Amphotericin B
• Antifungal Agents
• Azoles
• Fluconazole
• Flucytosine
• Fungemia
• Humans
• In Vitro
• Itraconazole
• Ketoconazole
• Miconazole
• Microbial Sensitivity Tests
• Mycoses
• Pyrimidines
• Triazoles
• Trichosporon
• voriconazole

Other ID:

• GWAIDS0024841

UI: 102264465

From Meeting Abstracts