2003 AND 2004 FOLLOW-UPS ON A 2002 UNIONID TRANSLOCATION FROM MISSISSIPPI RIVER MILE 818.9, COTTAGE GROVE, MN Marian E. Havlik Malacological Consultants, 1603 Mississippi Street, La Crosse, Wisconsin 54601- 4969 In 2003 and 2004 we conducted follow-ups of a 2002 mussel translocation from Mississippi River Mile 818.9, downstream from St. Paul, at Cottage Grove, Minnesota. The 52258 m2 area (1500 X 375 feet) extended from the LDB to the Main Channel. The translocation, done prior to burial of a wastewater disposal pipe, yielded a mean mussel density of 0.38/m2 among 19,630 live mussels (23 live plus 4 sub-fossil species). Of the 1439 (7.33%) mussels which represented two Minnesota endangered and five threatened species, at least 572 mussels were numbered, measured, and aged (1-17 years of age). All other living mussels were hash-marked. In May 2003, 609 live unionids (18 live plus 5 sub-fossil species) were recovered from the two Translocation Sites; 515 were numbered and hash- marked mussels. 76 Arcidens confragosus had a 98.7% survival, while 232 Quadrula nodulata had a 98.3% survival. One each of Tritogonia verrucosa, Obovaria olivaria, and Ligumia recta were recovered alive. The survival of numbered special status mussels was 98.36%; 12.6% of the numbered mussels had external growth ring disturbances. About 3% of numbered mussels showed little growth. The overall survival of all marked and numbered mussels was 97.2%. The substratum throughout both translocation sites was mostly mud with woody debris, and up to 6 m deep. In June 2004, 183 numbered Quadrula nodulata (98.9% survival), 58 numbered Arcidens confragosus (96.7% survival), and 2 numbered Obovaria olivaria (100% survival) represented 54.2% of 448 mussels recovered (15 live plus 1 dead [hash- marked] species). Of the numbered mussels, 81 (33.3%) were new to the follow-up measuring and external aging process. Nearly 98% of the numbered mussels survived in a muddy habitat, <1 meter deep. About 12.7% of the numbered unionids showed little or no growth; however, most of these were older mussels that would be expected to have a slow growth rate. At least 10.7% of the numbered mussels had external growth ring disturbances. In 2004, the overall survival of all hash-marked and numbered mussels was nearly 95%. Most numbers engraved on threatened and endangered mussels were still very legible. A total of 392 of 572 (68.5%) numbered mussels were recovered three times with an overall survival of 97.95%, in spite of an increased mussel density. Similar percentages of all age classes were recovered during each project year. Two years after translocation, the overall survival of all numbered and hash-marked mussels was nearly 96%. No numbered mussels ever moved into the sandy area upstream of Translocation Site 1. Slightly more Dreissena were found in 2003 than in 2002, but none were found dead or alive in 2004. Since several PVC pipes remained in the original project area in 2004, construction impacts were unlikely to have extended beyond the marked project site. Keywords: mussel translocation, external unionid aging, unionid substrata, Mississippi River, Threatened and Endangered mussels