ࡱ> ܥhc e8L44666666666666v67Cj7n7n7n7777777777778Xg8;7677"7777766n7j777776n7:677 '6666667777Project Report Fisheries and Oceans Canada Institute of Ocean Sciences Sidney, B.C., Canada To: Regional Director, Science Final: Yes 1. Cruise/Project No.: 2001-08 2. Dates: May 29 to June 28, 2001 3. Project Names: Haida Eddies, Argo and Line P Time Series 4. Area(s) of Operation: west of Queen Charlotte Islands & N.E. Pacific to Station P 5. Platform: John P. Tully 6. Master: John Anderson 7. Days Allocated: 32 8. Days at Sea: 29 9. Days lost to weather: 0.5 Days lost to other causes: 1 day to load, 1 day to offload 11. Appropriateness of platform: great 12. Safety issues: none 13. Cruise/Project Results (see appendix for details): Both the eddies and Line P surveys were very successful. Only minor sampling was omitted at Station Papa due to weather, otherwise all planned work was completed. The first 16 Argo drifters were deployed for the IOS program which intends to cover the Gulf of Alaska this year. Drifters are successfully reporting data back to MEDS. Surface drifters are showing that one of the eddies we sampled may be intensifying. Zooplankton sampling suggested that larval fish are being actively transported from the coast to open ocean along the edge of pairs of eddies at the south and north end of the Queen Charlotte Islands. Work completed included: surveys of Haida-2001 and Haida-2000 eddies (SSF funding), with sampling for water properties (T, S, O2, nutrients), phytoplankton (primary productivity, species, pigments), zooplankton (bongo tows, Bioness tows, optical plankton counting), biogeochemical transport (carbon, nitrogen, silica budgets) and trace metals (iron and zinc). Three surface drifters and several profiling floats were deployed in and near eddies. deployment of 16 Argo profilers between 49 and 53N, the coast and 145W. Profilers have been reporting their data through Argos to MEDS. survey of water properties and zooplankton along Line P (SSF funding) recovery and deployment of 4 sediment traps at stations P26 and P4 on Line P (Wong) primary productivity measurements along Line P (UBC). iron distribution and speciation along Line P (Sutherland) zinc distribution and speciation, incubation studies to look at impact of Zn and Fe on phytoplankton growth (Lohan and Crawford, SOC) coccolithophore sampling in surface waters on both legs, to examine distribution vs oceanographic properties. opportunistic survey of Dickins Seamount on the edge of Haida-2000 (Dower). opportunistic sampling for trace metals in vicinity of Tully eddy near the mouth of Juan de Fuca (Marchetti) 14. Primary Institute: Institute of Ocean Sciences, DFO 15. Associated Institutes: University of BC, Canadian Wildlife Service, U. Southampton (SOC) 16. Chief Scientists/Affiliation: Frank Whitney, DFO, OSAP Division, IOS Science Staff/Affiliations: Eddies (May 29 to June 13) Marie RobertIOSKeith JohnsonIOSDoug AndersonIOSNes SutherlandcontractDave MackasIOSTawnya PetersonUBCLisa MillerIOSHugh MacleanUBCDoug YellandIOSJohn DowerUBCJennifer PutlandIOSSabrina CrispoUBCMichael ForceCWS contractBill CrawfordIOSDavid CrawfordSOCMaeve LohanSOC Line P: Marie RobertIOSWendy RichardsonIOSBernard MinkleyIOSNes SutherlandcontractTim SoutarIOSTawnya PetersonUBCDarren TueleIOSHugh MacleanUBCMike ArychukIOSAdrian MarchettiUBCJennifer PutlandIOSChris MayseUBCKen MorganCWS David CrawfordSOCMaeve LohanSOC 18. Equipment: ADCP: yes Other: multi-frequency sounder, thermosalinograph, Bioness and bongo nets, optical plankton counter, trace metal clean pumping system, on deck incubators. Onboard analyses included nutrients, oxygen, salinity, iron, zinc, dimethyl sulfide, chlorophyll, pH and pCO2. Winches: . typeID NoWire typeWire Condition/SpoolingNo Casts/ Depth max32913073640 m CTDwire is greasy, winch surged50/4200m32914834710 m CTDgreasy wire, winch surged 20/3500m32912330.325 Bionessgood12/25032012075/32good30/1504551151user supplied4Grease oozed from CTD cables, contaminating rosette so that organic carbon samples could not be collected and we had difficulty with other samples due to grease on spigots. Winches both surged above 1 m/s on down casts. Slow descent speeds lead to the rosette cycling up and down during casts in moderate weather. We recommend that the policy of greasing cables be reviewed and ended if possible. Equipment Deployed/recovered DescriptionDeploymentRecoverymoored sediment traps44 Comments and recommendations: Thanks to the chief engineer for helping us with winches. Some of our sampling (dissolved and particulate organic carbon) was moved from rosette to Go-flos because of the heavy coating of grease on CTD cables. Moving to Go-flo sampling affects the precision of this work (less detail, no supporting data from the CTD) and the time it takes to collect samples. Much of our foul weather gear has been soiled and the ship in general becomes sullied as we bring the grease on our clothing and footwear into living spaces. We are beginning to see problems with the number of staff that are able to support field programs. While onboard, we had to unexpectedly try to find a way to support the following cruise. This means that one of our people (Marie Robert) will head out for an additional 10 days at sea following a 30 day cruise. The work load is becoming too large for the current staff, and this problem is going to escalate as personnel retire in the next 2 years (Minkley and Soutar appear decided). As always, the Tully crew were very helpful in assisting our work. Appendix: Reports from Participants Chief Scientist Report Frank Whitney This cruise consisted of 2 legs, the first undertaking surveys of two Haida eddies and the second continuing our time series measurements along Line P (see the cruise track, below). Both legs were very successful, accomplishing all major aims and adding some opportunistic sampling. Between the 2 legs, scientific personnel were exchanged in Masset on June 13.  Sampling for basic water properties was enhanced this cruise with a new Seabird oxygen probe which provided a detailed survey of oxygen over a broad area in the Gulf of Alaska. Nutrient surveys along about 53N and Line P show that we are not experiencing the impoverished conditions of the mid to late 1990s. However, a pool of warm water remains below the mixed layer throughout the transition waters along Line P. This water is relatively poor in nutrients compared with subarctic waters of a similar salinity or density. The participants (ships crew and scientists) on both legs of this cruise were a cooperative and enjoyable group. Thanks to all. 2. Primary productivity T. Peterson, J. Putland, S. Crispo I. Eddy survey Objectives. We sampled two major stations (centre and edge) in two eddies (Haida 2001, Haida 2000) as well as a reference station outside in order to examine how eddies may differ from surrounding waters in terms of the primary producers. By re-examining Haida 2000 we may gain information about how biological communities evolve over time within these large eddies, and what impact this may have on regeneration/flux of nutrients and carbon in the Gulf of Alaska. Sampling. We drew samples from the upper 6 light depths corresponding to 100, 55, 33, 10, 3, and 1% of surface irradiance. Rate measurements. a) We used the 14C-uptake method (24h incubations) to estimate primary productivity. These samples were size-fractionated (> 20 ?m, 5-20 ?m, 0.2-5 ?m), to characterize variations in productivity between different components of the phytoplankton community. These samples will be split into organic and inorganic fractions. Size-fractionated chlorophyll a was determined to allow normalization of productivity measurements, and to estimate biomass present in the different size classes. b) Uptake of nitrate and ammonium will be determined from 15NO3 and 15NH4 uptake experiments at each of the major stations. This will allow estimation of the amount of new production (nutrients from some outside source vs. recycled) inside versus outside the eddy. c) Bacterial productivity will be estimated using the 3H-Thymidine uptake method. d) Photosynthesis versus irradiance relationships at 3 depths per station in Haida-2000 and 2 depths per station in Haida-2000 will be used to examine how efficiently phytoplankton cells are assimilating carbon. Phytoplankton community. Samples were taken to perform pigment analysis using High Performance Liquid Chromatography to better characterize the phytoplankton community, particularly the smallest cells that are difficult to identify using light microscopy. Preserved samples were taken, and will be enumerated in the laboratory. In addition to these measurements, samples were also obtained for nitrogen stable isotope analysis to see if ?-15N signatures may differ inside versus outside the eddy, as well as for biogenic silica concentration and flow cytometric analysis. II. Competition experiment A simple phytoplankton competition experiment was set up to see how organisms from inside the eddy would compete with those outside, in order to simulate what would occur as eddies spin down and mix with oceanic waters. Water was collected from the centre of Haida-2001, from the outside reference station, and the two were combined for a third treatment. The three will be compared for primary productivity, nutrient utilization, biomass (chlorophyll, particulate carbon/nitrogen), and species composition (preserved samples and HPLC) over a 9-day period. III. Methodology A test was conducted to optimize the method we had been using to determine biogenic silica (Putland). Biogenic silica concentration is most accurately determined when pH does not exceed 6-6.5. At higher pH the concentration of biogenic silica is underestimated. IV. Line P leg Total primary productivity and chlorophyll a will be determined for stations P26, P20, P16, P12, and P4 using the 14C-uptake method. 6-hour and 24h incubations will be compared in order to determine whether the shorter incubations accurately reflect daily uptake rates when values are extrapolated to 24h. Samples were also taken for species enumeration and identification. 3. Dimethyl Sulfide, Line P M. Arychuk, Institute of Ocean Sciences, Canada General: DMS samples were collected and analysed at stations P4, P12, P16, P20 and P26(Papa). Three casts were taken at P20 to represent a diurnal data set. Rosette casts consisted of 13 samples taken at the following depths (in meters); 200, 175, 100, 50, 40, 35, 30, 25, 20, 15, 10, 5, & 0. There were no adverse data quality issues and all implemented QA/QC gave acceptable results. Results: In all cases there was no DMS below 175m and values increased steadily at the 50m depth to the surface. This is consistent with historical data sets, however, interesting data was gathered at stations P04 and P12 where some of the highest ever concentrations of DMS were observed at the sub-surface levels. The diurnal cast was done at station P20 this year due to unfavourable weather at station P26, but despite the location change, results were representative of previous diurnals done at station Papa. Overall, the results obtained for DMS during this cruise have provided some consistent and predictable observations, but also some very unique profiles that have never before been observed. Obviously further investigation will be needed before one can expand on such observations or determine how they relate to other chemical processes or biological activity in the area. 4. Haida Eddy Cruise - Metals Sabrina Crispo University of British Columbia Continuation in sampling for trace metal analysis in the Haida eddies was performed to give information on changes in trace metal concentrations over time (specifically for aluminum, gallium, manganese and cadmium). Also, initial metal concentrations will be used to trace source water and hopefully give information on iron sources. Seawater was collected for trace metal analysis at the major eddy stations (edge, center and reference) in Haida 2000 and 2001 at depths of 10, 25, 40, 100, 150, 200, 300, 400, 600, 800, and 1000m. Collection performed off the chains using Kevlar line. A Teflon pump was used for 10, 25 and 40m sampling; sampling at other depths using 12L and 30L Go flos. In addition to this sampling, a 10m sample was obtained at all stations (ED01 to ED30) to provide more information about horizontal spatial variations. The seawater collected was acidified and will be returned to the University of British Columbia to be analysed. 5. Drifting Bill Crawford I deployed three WOCE-type surface drifters in Haida-2001a, launching one during the initial South-North transect, and the remaining two during the following East-West transect. Wind speeds were exceptionally low during the first 10 days of drifter tracking, allowing clear interpretation of eddy surface-current speeds. Tracks of these drifters showed them to be about 10 to 15 km from the eddy centre, and 120 degrees from each other. All circled the eddy in a clockwise direction, at speeds between 0.1 to 0.2 m/s. Each drifter also followed smaller gyres that seemed to be inertial-period loops, also of speeds 0.1 to 0.2 m/s. The tracks confirmed the clockwise motion inferred from satellite altimetry. However, the magnitudes of geostrophic current computed from sea surface height gradients is slightly less than 0.1 m/s, smaller than the average eddy speeds determined from drifter tracks, after eliminating the inertial currents. I believe the contour maps of sea surface height may have smoothed out the sea surface slope of the eddy at this distance from the centre, contributing to the small geostrophic speeds. By the end of leg 1 of this cruise, the first drifter had quit transmitting, but the remaining two were operating. Although none had completed a circuit, it appears that one circuit at 10 to 15 km from the eddy centre takes about 10 days. It is too early to determine any net inward or outward motion of the eddy waters at surface, associated with eddy decay. Altimetry images show this eddy may be merging with another eddy to the northwest; therefore the drifter tracks in following weeks may be dominated by this process rather than a slow decay of Haida-2001a. Other general comments: Grease on rosette winch wire is a nuisance, and even appears to be contaminating samples. If left aboard it will eventually be tracked through the ship. A working ADCP would enable better tracking of drifter core. In future, if drifters are to be launched, it may be economically feasible to download drifter tracks directly to ship. Recommendations for future work: Now that we have completed a cruise with deep CTD profiles, the next project might be to undertake shallower profiles with more rapid sampling using only the CTD, with a transmissometer and O2 probe. This idea was proposed by Frank Whitney. Profiles to 200 m from the ship while underway, along with ADCP profiles and drifter tracks, would provide information on the finer structure of the eddy motion and mixing. If the CTD could be lowered at a smooth rate with the sensors mounted pointing downward, below any other apparatus (including weights), it would be possible to compute overturning scales associated with mixing, and thereby determine any turbulence properties. We would also learn from a two-day time series at a station following a drifter track. 6. Carbon sampling and analyses L. Miller, D. Crawford In our continuing efforts to understand carbon cycling in Haida eddies and how they evolve with time, we collected a comprehensive set of organic, inorganic, dissolved, and particulate carbon samples throughout both the Haida 2001 and 2000 eddies. In particular, we collected full profiles of dissolved inorganic carbon (DIC), alkalinity (AT), and pH from the rosette casts at 6 stations: the central, edge, and reference stations, as well as at ED18, the last station on the east-west transect of Haida 2001, which we consider to be representative of the open ocean waters into which that eddy is moving. We also collected dissolved organic and particulate carbon (DOC and PC, respecitively) samples from Go-Flos deployed off the starboard side of the ship at all the major central, edge, and reference stations, for a total of 5 stations. Samples from deeper than 100m were collected in conjunction with the trace metal casts, while those from shallower than 100m were collected during the primary production casts. In order to allow eventual mapping of pCO2 distributions and estimates of air-sea CO2 fluxes, additional DIC, AT, and pH samples were collected from a rosette bottle triggered at 10 m at all stations. At cruise's end, only the pH samples had been analyzed, and the data for those have not yet been processed. However, a very preliminary perusal of the raw pH data indicate only subtle horizontal variations in pH. 7. Domoic Acid studies, Line P Adrian Marchetti At Station Papa, the UBC group consisting of Adrian Marchetti, Chris Mayse and Tawnya Peterson performed several Fe manipulation experiments. The first experiment implemented was to study the effects of varying levels of domoic acid in conjunction with undersaturating Fe additions to Station P phytoplankton. This experiment carried on for 6.5 days with sampling occuring at time points 0, 24, 48, 96, 144 and 156 hours. Water from a depth of 15m was collected by a teflon pump system and placed into acid-cleaned cubitainers and inoculated with various concentrations of Fe and domoic acid. The purpose of this experiment was to investigate the possible role of domoic acid as an Fe chelator for Pseudo-Nitzschia spp., the predominant phtyoplankton genus with the addition of Fe to St. P waters. Analysis of samples included Fe uptake, 14C uptake, chlorophyll (size fractionated and total), POC/PON, phytoplankton species, bacterial productivity and nutrients. A side investigation looked at the production of DMS by Fe-induced phytoplankton. Analysis included all of the previously mentioned plus DMS, HPLC and flowcytometry. An additional experiment was incorporated to measure the possible production of domoic acid in diatoms at St. P. Samples were inoculated with nutrients or iron and incubated for 40 days. Analysis of samples included chlorophyll a, phytoplankton species composition and domoic acid presence/absence. This experiment will be ongoing at UBC. Fe sampling was also performed in the region surrounding the Tully Eddy. Three stations were sampled for total and dissolved Fe using trace-metal clean Go-Flos and kevlar line. At station 1 (48 23 069 N. 124 20.403 W) samples were drawn from 10, 40 and 100m. At station 2 (48 32.035, 125 35.480W) samples were drawn from 10, 25, 40 and 70m. At station 3 (48 31.723N, 126 12.108W) samples were drawn from 10, 40, 100 and 400m. Much appreciation and thanks goes to the IOS team for the assistance in sampling and analysis of nutrients and Fe, as well as their patience and willingness to teach sampling techniques. 8. Iron Meassurements Keith Johnson and Nes Sutherland Eddy leg Trace metal sampling for iron, cadmium and zinc were carried out using the teflon tubing and pump system for 10, 25 and 40 meters and Go-flo bottles on 1020 meter Kevlar line for depths of 75, 100, 150, 300, 400, 600, 800, and 1000 meters. Six profiles (ED01 to 600 meters only, ED10, ED15, ED19, ED25 and ED28 ) and numerous 10 meter samples were collected. Most of the 10 litre were for Cadmium only with 5 for iron and zinc as well. Filtered samples were collected for all three metals using a 0.2 micron cartridge from Milli-pore (Opticap). Unfiltered samples were also collected for iron only, as well as 0.03 micron samples (Steropore) from pump depths only. Reactive iron filtered and unfiltered were analyzed onboard with acidified samples for total iron returned to the lab for subsequent analysis. Line P Leg An iron sampling protocol very similar to the Eddy leg was followed for the 5 major Line P stations P26, P20, P16, P12 and P04, as well as an extra Z03 station closer to the center of the Alaska gyre. Sampling depths were 0, 10, 25, 40, 75, 100, 200, 300, 400, 600, 800, and 1000m, with filtered and unfiltered acidified samples taken at all depths (to be analysed at IOS). Due to time constraints, however, the full profile of reactive samples was analysed only at stations Z04, P26 and P20. Reactive iron for the remaining stations was limited to sampling the upper 400 meters. Iron near surface (0 to 40m) concentrations were found to be less than 0.1nM for all Line P stations. 9. ARGO FLOATS DEPLOYMENTS Marie Robert During cruise 2001-08, 16 Argo floats were deployed. These floats are actually drifters that spend most of their time at 2000 dbar and come up to the surface every 10 days. On their way up from 2000 dbar they perform a CTD cast (temperature and salinity profile). Once at the surface they transmit their position and CTD data through Argo satellites. The data is then decoded by MEDS in Ottawa. The goal of this cruise was to cover a grid along and north of Line P. We also deployed 2 floats in the centre of 2 eddies studied during the first half of this cruise. The 16 floats were deployed without any problems. For each float deployed a CTD cast has been obtained from the instrument on board the ship for calibration and data quality purpose. The data received so far from the floats are in perfect agreement with the CTD data obtained from the ship. Here is the location of the float deployments:  10. SEABIRD and MARINE MAMMAL SURVEY 14 26 June 2001 Ken Morgan, CWS All bird and mammal surveys were conducted from Monkey Island or during bad weather, from the ships Bridge. The position and time that observations began were noted and at hourly intervals. Normally, the sea was scanned in a 250m radius arc, centered on the bow. Birds outside of the survey zone were not tallied unless they flew into the zone. Birds were recorded in 5-minute bins each bin being 500m wide by the distance traveled during each 5-minute increment (areas to be calculated). When observations were conducted from within the bridge, only one quadrant was surveyed (i.e. total width 250m). All identifiable marine mammals observed were noted regardless of distance from the vessel. Table 1 summarizes the survey effort each day, sea state (average Beaufort Scale), number of bird species observed, total number of birds and number of marine mammals detected. The diversity of species varied from day to day as did the total number of birds. In terms of overall bird numbers, very few birds were encountered during the first 7 days of the cruise. It was not until we had traveled east of 136 degrees that the numbers started picking up. Although I need to compare these results with the data from other early summer trips (1996 2000) I suspect that these are the lowest numbers encountered so far. On the last survey day (June 26) more than 80% of the total number of birds were encountered. Table 2 shows that five species: Sooty Shearwater, Leachs Storm-Petrel, Fork-tailed Storm-Petrel, Black-footed Albatross, and Glaucous-winged Gull accounted for more than 96% of the total birds seen. Overall, and despite the lack of birds, the cruise was highly successful and enjoyable. Because of the slope of the housing, the observer is always being buffeted by winds this is the one and only drawback to observing aboard the Tully. I will be investigating the possibility of building an observers shelter to be affixed to the deck of Monkey Island. Table 1. Dates, survey location, effort, sea state, bird species diversity, total birds observed and numbers of marine mammals encountered, 14 26 June. Date JuneStart Psn.End Psn.No. of TransectsAve. BeaufortNo. Bird Spp.No. of BirdsNo. of Dalls PorpoiseNo. Pacific White-sided DolphinNo. Harbour PorpoiseNo. of Fur SealsNo. of Humpback Whales1453.48x135.4553.30x137.29524.2597000001552.59x140.4253.00x148.08542.0420000101652.49x145.0452.44x145.0783.032000001751.03x145.4550.36x145.26404.047400001949.58x144.5949.59x144.26284.3522400002049.50x142.3849.41x140.21644.5788000002149.34x138.4149.33x138.26125.0420000002249.25x136.2349.18x134.49684.55181400002349.14x134.0849.03x131.47761.46432700102448.59x130.3848.52x129.22343.9576500002548.44x127.3548.39x126.41440.793490500102648.39x126.3948.33x125.03671.7956651067105Grand Total54721695934117115 Table 2. Total numbers of birds observed and the percentage of the overall number of birds. SpeciesNumber%Black-footed Albatross1041.49Northern Fulmar240.34Sooty Shearwater555679.56Short-tailed Shearwater10.01Pink-footed Shearwater520.74Mottled Petrel420.60Murphys Petrel10.01Leachs Storm-Petrel6679.55Fork-tailed Storm-Petrel814.38Double-crested Cormorant10.01Pomarine Jaeger10.01Parasitic Jaeger40.06South Polar Skua30.04Phalarope spp.40.06Glaucous-winged Gull811.16Unidentified gull sp.20.03Common Murre30.04Tufted Puffin260.37Horned Puffin30.04Rhinoceros Auklet300.43Cassins Auklet440.63Ancient Murrelet30.04Unidentified alcid sp.250.36 11. Studies on Dissolved Zinc David Crawford & Maeve Lohan, University of Southampton, UK The first main focus of our work was to take vertical profiles of samples for total dissolved Zinc (Zn) in the waters of the subarctic north Pacific, and to conduct Zn speciation measurements on as many of these samples as possible. The speciation measurements will tell us what proportion of the total dissolved Zn is in the free ion form Zn2+ and what proportion is complexed to organic ligands; this is of potential importance with regard to growth of phytoplankton in this area. Our clean trace metal sampling was conducted in close collaboration with Nes Sutherland and Keith Johnson who were sampling for dissolved Iron (Fe). The second focus of our work was to conduct an on-deck experiment incubating cubatinors of water from Ocean Station Papa with various combinations of Fe and Zn supplementations. The incubations were then monitored for various parameters of phytoplankton growth. Our work was divided into two portions: Eddy Leg During the eddy leg of the cruise we took vertical profiles of dissolved Zn at discrete depths from the surface down to 1000m. These profiles were taken from the centre of the eddies, from the edge of the eddies, and from the reference station. From a limited number of depths from these profiles we conducted speciation measurements. We also took horizontal transects of samples from 10m depth across the eddies, both for total Zn and for speciation. These measurements will be used in order to assess whether there are variations in Zn availability inside and outside of eddies; the Zn data will be integrated into the general collaborative data set for the eddy work. Line P Leg Along line P we took vertical profiles of dissolved total Zn at each major station (P26, P20, P16, P12, P4). Each profile consisted of samples from 0, 10, 25, 40, 75, 100, 200 & 400m. Speciation measurements were not conducted along line P because of the concurrent need for speciation measurements from the incubation study. However, these speciation measurements may be conducted by Maeve Lohan along line P during the Aug/Sept 2001 cruise. The enrichment incubation experiment consisted of pumping (teflon pump) trace clean water from Ocean Station Papa into clean cubitanors and adding one of four Fe/Zn combinations: Control + 10nM Fe + 10nM Zn +10nM Fe + 10nM Zn The cubitanors were then incubated for 8 days at ambient seawater temperature in incubators on the helicopter deck. Cubitanors were sampled each day for total Zn & Fe, Zn speciation, total CO2, total alkalinity, del13C, nutrients, chlorophyll, HPLC pigments, phytoplankton species composition. The purpose of these analyses was to investigate the potential influence of adding Zn on phytoplankton growth, species composition, CO2 drawdown, and discrimination against 13C. 12. Haida Eddy and Triangle Island zooplankton, larval/juvenile fish, and acoustic sampling D. Mackas, D. Yelland, J. Dower 1. Sampling methods, numbers of samples, and methodological successes/problem areas a. Vertical hauls with bongo nets (0.23 mm mesh) Vertically-integrated bongo tow samples were collected at all stations. Samples from one side of the paired net were preserved in 10% formalin/seawater for taxonomic identification and enumeration. Samples from the other net were frozen for biomass measurement and potential chemical analyses. For the Cape Scott Station Line (31 May, 15 samples total) we sampled two overlapping depth ranges (0-50 m plus 0-near bottom or 0-250 m). For both the two Haida Eddy surveys and their offshore control sites (2-12 June, 42 samples total), and for Line P/Stn P (16-26 June, 5 samples total), bongo tows were 0-150 m. Because the starboard side hydro winch was fitted with fragile kevlar rope needed for the trace metal sampling, all bongo tows were done from the stern A-frame. This can be a very good location from which to do bongo net sampling. However, our initial arrangement of winch (port side) and cable run to block (port side wing of A-frame) allowed major problems because pitching of the ship caused frequent slack cable during lowering of the net, and intermittent loss of contact between the wire and the winch counter pulley. On one station, a 15-20% error in the wire-out reading caused us to drag the net along the bottom, damaging the net and producing a very low quality sample. Visibility between winch and stern was also relatively poor because of the intervening spooling winch and container lab. To remedy this, TULLY crew subsequently re-routed the towing cable along and across the stern deck (using two deck-mounted snatch blocks) to the starboard wing of the A-frame. This configuration was a great improvement: reduced/absorbed cable slack, provided much better angular contact with the winch counter pulley (about 25% vs <5% of circumference), more working room at the stern, and better visibility from the winch. The prinicpal drawbacks were that, during net tows, we were crossing the deck with moving cable at ankle-to-knee level. This required extra care for staff accessing the CTD/rosette, and for the midline EM cable leading to the CTD/rosette. b. Stratified-oblique tows with BIONESS (0.23 mm mesh) and externally-mounted Optical Plankton Counter (OPC) Day and night tow pairs were done at each of the Haida Eddy 'core' and 'margin' stations (ED08, 15, 25, 28) and at the 'outside-eddy control' station (ED19). Each tow sampled 8 depth strata: 0-250 m (sorted live for fish and invertebrate larvae, and for zooplankton subsample for stable isotope analysis); 250-150, 150-100, 100-75, 75-50, 50-25, 25-10 and 10-0 m (all preserved in 10% formalin/seawater for taxonomic identification and enumeration). BIONESS deployments were through center of the stern A-frame, using the same block as, but a different cable and winch than, the CTD/rosette. All tows went extremely well, thanks to recently updated BIONESS hardware and sortware. The only logistic problem was in switching back and forth between CTD/rosette and BIONESS winches and their respective grease-laden cables. Despite our efforts to minimize contact with the cables, each switching operation resulted in extensive grease contamination of scientific staff, instruments, and subsequent samples. c. Surface horizontal tows with neuston net (0.5 mm mesh) Surface tows (~15 minutes at 3 kts) for taxonomic and stable istope analysis of fish and invertebrate larva and zooplankton, were done at the Haida Eddy survey stations which were occupied during the relatively brief periods between dusk and dawn (12 stations total). Samples were live sorted onboard into fish, macro-invertebrate (crab and squid larvae), and zooplankton categories, sieved and then frozen (similar samples were sorted from the 0-250 BIONESS samples). Bulk filters for surface particulate organic matter were obtained at the abovee sites, and also frozen. Deployments of this lightweight net were done from the starboard chains using the kevlar rope on the hydro winch, and were low-tech and essentially problem free. d. Multi-frequency echosounder Instrument trials were done with a new three-frequency echosounder system (40, 100 and 200KHz)hooked to the TULLY's hull-mount transducers. The main purpose of this work was to gain familiarity with and debug the new electronics and software, and the existing transducer cabling, but we did obtain extensive transect data that will provide some information about variation in backscatter intensity along the survey tracks. (150KHz backscatter profiles are also output by the Acoustic Doppler Current Profiler, see separate ADCP report by Yelland) The main problems identified were pickup of and data contamination by line frequency noise (60 and 120 Hz), and software/hardware conflicts during transfer of multi-Gbytes from hard drive to mag tape. 2. Results a. Cape Scott line zooplankton This section was done in support of a multi-year program studying oceanographic influences on the Triangle Island seabird colony. The main goal is to identify year-to-year variations in foraging site and quality for planktivorous Cassin's Auklets. Zooplankton community composition and distribution differed from late spring observations in previous years. Along the NE half of the line (Queen Charlotte Sound), crustacean zooplankton were relatively abundant, and foraging auklets were observed. Along the SW half of the line (Vancouver Island continental slope, and the 'normal' primary foraging region for the seabirds), the zooplankton were unusually, and overwhelmingly dominated by very high concentrations of salps. Few seabirds were seen in this region. b. Haida 2000 eddy zooplankton At all of the Haida Eddy survey sites (within both H2000 and H2001 eddies and at the offshore 'control' stations), about 50-90% of the zooplankton in the upper 250 m consisted of late juvenile stages of the large Neocalanus spp. copepods. These were located primarily within the upper 50 m both day and night. Each spring season, early juveniles of these species migrate upward into the surface layer of the subarctic Pacific from the deeper (400-1200 m) strata at which they were spawned in late winter. We plan to test for possible effects of the eddy on their abundance, body size, lipid accumulation, and developmental rate. For the H2001 eddy (centered near 51.25N, 134W), Neocalanus abundance appeared to be somewhat higher at the eddy margin than either ouside or in the core of the eddy. This result was, similar to what we observed last spring in year 1 sampling of H2000. A new and quite striking signal was that, around the margin of H2001, the background copepod population was augmented by high concentrations of salps, suggesting that the eddy was 'ringed' by entrained water of slope region origin (see below). Within the core of the H2001 eddy, we also found elevated concentrations of larvaceans and early juvenile euphausiids (both potential shelf/slope tracers), but we did not find the high concentrations of shelf-origin Pseudocalanus that we observed in spring 2000.  c. Haida 2000 zooplankton As noted above, Neocalanus spp. were also the dominant zooplankton in H2000 (centered near 54.5N, 138.25W). Salps were nearly absent. The main distinguishing feature of the 'within-eddy' zooplankton was greatly enhanced abundance of copepods in the 100-250 m depth strata. Many of these were taxa (e.g. Metridia pacifica) that have little or no seasonal vertical migration, but a relatively strong diel vertical migration. Our present hypothesis is that anticyclonic eddies may aggregate or trap these diel migrants. d. Larval/juvenile fish and macroinvertebrates. As expected, larval, juvenile and adult myctophids were relatively abundant at all locations (adult myctophids are strong diel migrants and dominant members of the open-ocean midwater micronekton community). Considerable interest was generated when we found 2-3 cm fish that looked like early juvenile pink salmon at several stations along the S flank of H2000. However, we now think that these are not salmon (what they are remains to be determined). Sablefish (Anoplopoma fimbria) larvae and early juveniles (1-3 cm body length) were abundant in many of our eddy samples. Sablefish spawn along the continental slope and, like the salps mentioned above, are likely to be indicators of entrained slope-origin water. Eddy stations also contained crab megalopae (Cancer sp?), a few early stage rockcod juveniles (Sebastes sp.), and higher numbers of squid larvae. 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Jun 14 to 28, 2001 ? )6surface samples taken from the Niskin bottle at ~ 10 m* "!(""#,,$"$$++B..B.. stnserial # yy mo day utc utc   PDT _Latitude _ d.Lat _ Longitude_d.longd.LatCTD T CTD Bot NO3 PO4 Si Si:N ChlCoccocomments %%% hhmm datehh:mm zone  date  dd mm.mm   dddmm.mm  Sal Sal uM uM uM ug/L #/L!!!B ..!!!@loop135 601 606~ 6,@ 603007@A?9?' :@ DDD 5K@;@ & ;1Z$K@D <D  5`@;õ@&;ʡE`@D<D ;1Z$K@D <BB=Q>(K#?>u-1?=VLI@!> c"@ DD?ocN@??!!!4loop145 601 606~ 5,@ 60700~ 7@8?9?' :@ DDD~  5K@ C09.61& ;aWK@D <D  5`@;Q@&;7B`@ D<D ;aWK@ D <<<=NA~6@>MC']?= T*@!>b@  DD?<e???%%% @loop15 5 601 606 5,@68@7@ 8ll? 9?' :a `@ D D D  5J@;@ & ; J@ D <D  5`@;L@& ; `@ D <D  ; J@ D <<< =]].@ >m6? =/g^"@! >JV?  D D  ?Q7 ? ??%%% 4loop16 5 601 606 5,@5t@7@ 8[[? 9?' :[ @ D D D  5J@;@ & ;g J@ D <D  5`@; @& ;zG`@ D <D  ;g J@ D <<<~ = >C8fٓ? =%7@ > D^~T? 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I@!!iJُH@" I@" eH@#I@#K~|H@$MbI@$d;OwH@%MbI@%MrH@&x&1I@&!rhmH@'y&1I@' rhH@(3 I@(贁NcH@)A tڸI@)g _H@*<'J@*g _H@+Mb`J@+A tXH@,MbJ@,PnSH@-#J@-<'OH@..$M8J@.LH@/ޖ K@/K7IH@0Mb K@0Zd;OEH@1'5K@1:m:H@2JK@2fffff&H@3\HJK@3o!H@4Mb`K@54 {uK@6|ͅ)K@7QK@8^rK@9b/FK@:bWkFK@;ƒ_,EK@<(\EK@=(eFK@>x&1@K@?WAK@@CҔ?K@e> @CҔ  SummaryInformation( DocumentSummaryInformation8_1056789106 Fie'n'Ole  ՜.+,D՜.+,4 PXd lt| DFO1 acoastoutla lo  WorksheetsCharts 6> _PID_GUIDAN{20DCB860-392B-11D4-8D85-005004748898} "----'--- p "-  "----'--- p---'--- s---'---  Arial--------'--- ^  '2 Cruise Track, 2001-08     (2 =May 31 - June 12, 2001      ----'--- ---'--- ----'---   2 j50 2 `j51 2 j52 2 j53 2 /j54 2 j55 ---'--- ---'---   2 P127 2 129 2 131 2 x133 2 135 2 =137 2 139 ---'---  Arial- - - ---'- -- G 2 JLongitude (W)  - ---'- -- - - ---'- --   Arial- 2  Latitude (N)-  - ---'- -- ---'- -- - - 45- "-!)v- --- -  Arial- -  2 Salps(! !' --  - '  '  '2 (#+2 (P"2 ((% FMicrosoft Excel ChartBiff8Excel.Sheet.89qOh+'0@H`x  Robert Mariex Robert MariexMicrosoft Excel@rd@CompObj bObjInfo Workbook'SummaryInformation(      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|} IBa= <?=hL,8X1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial1Arial"$"#,##0;\-"$"#,##0"$"#,##0;[Red]\-"$"#,##0"$"#,##0.00;\-"$"#,##0.00#"$"#,##0.00;[Red]\-"$"#,##0.005*0_-"$"* #,##0_-;\-"$"* #,##0_-;_-"$"* "-"_-;_-@_-,)'_-* #,##0_-;\-* #,##0_-;_-* "-"_-;_-@_-=,8_-"$"* #,##0.00_-;\-"$"* #,##0.00_-;_-"$"* "-"??_-;_-@_-4+/_-* #,##0.00_-;\-* #,##0.00_-;_-* "-"??_-;_-@_- 0.0000                + ) , *       @ "P* "T* !P Chart1`kChart2 va b vcTU377~3  @@  mLatitude LongitudeSI03P1P2P3P4P5P6P7P8P9P10P11P12P13P14P15P16P17P18P19P20P21P22P23P24P25P35P26Vancouver Islandd.longd.latQueen Charlotte Islands Kodiak IslandPrince of Wales Island$Baranof and Chicagof (Sitka) IslandsKupreanof IslandAdmiralty IslandCoastStations4834.512530.04836.012600.04837.512620.04839.012640.04841.512710.04844.612740.04846.612810.04849.012840.04851.412910.04853.612940.04856.013010.04858.213040.04902.613140.04907.413240.04912.013340.04917.013440.04921.013540.04926.013640.04930.013740.04934.013840.04938.013940.04942.014040.04946.014140.04950.214240.05000.014336.314418.214500.05131.245000.004835.91 12329.94RI15126.39 12738.24RI2 12733.58RI35135.88 12732.00RI45138.88 12726.53CS105116.52 12820.04CS95112.49 12827.90CS85108.46 12835.96CS75104.39 12844.09CS55055.94 12900.21CS45049.23 12913.05CS35045.60 12920.07CS25041.32 12928.00CS15034.90 12941.50ED015015.00 13349.95ED025030.01 13349.92ED035044.98 13349.89ED045054.95 13350.01ED055104.98 13349.98ED065114.98 13349.99ED075124.98ED085134.99ED095145.015144.97ED105200.00ED115215.15 13350.00ED08bED12 13230.03ED135114.99 13259.98ED145114.93 13330.01ED15 13359.95 13359.89ED15w5115.04 13414.97ED165115.00 13430.04ED175115.06 13459.98ED18 13529.96ED195244.98 13700.005244.99ED205314.99 13809.96ED215335.05 13810.09ED225355.05 13809.87ED235404.98 13809.95ED245415.00 13810.05ED255424.83 13810.135424.78ED265435.04 13809.88ED275445.02 13810.02ED285455.02 13809.985454.98 13809.935454.94 13809.86ED295510.01 13810.04ED305530.02 13809.97ED315432.78 13730.15ED325433.16 13707.97ED335432.93 13654.00ED345432.76 13645.47ED355432.99 13620.20ED365429.98 13529.98ED375429.81 13359.84Argo75259.93 13959.92Argo85300.04 14230.01Z35300.00 14459.975259.96 14459.88PR15135.05 14534.89PR25110.00 14550.03 14500.155000.01 14500.39Stationlegendrosettes Argo floats5035.52 12940.445113.89 13349.785214.98 13349.945245.31 13700.455425.55 13814.055430.06 13533.295300.53 13956.735259.98 14226.865259.66 14503.405000.22 14418.504950.10 14239.814957.09 14500.864950.03 14239.984945.95 14140.104946.00 14139.994941.98 14039.864937.92 13939.934934.01 13840.074938.04 13939.624926.08 13639.73 13840.014933.91 13840.034934.08 13840.144930.07 13740.10 13640.074921.05 13540.054916.94 13439.904916.97 13440.014912.01 13340.104912.20 13340.114907.50 13239.934902.61 13140.004858.21 13040.014838.96 12639.894858.45 13039.954844.42 12739.634858.244856.03 13009.934853.57 12939.904851.35 12910.064849.08 12840.124846.55 12809.964844.58 12740.014841.64 12709.944838.93 12639.934837.45 12619.96 Bristol Bayj C  oO /   '+ {  #' w~  ghFPrS@>9(+{ly@jr4 tp_ tpb hβ @R X  p_ !k  #s ̀ , 8  @Lb{Aprrb{ApApLr@b]rrb pdlb.eeb ebb{ApWBApLb} erpAp errtprȐrbzeb errr$berrƸe eܱr0r?sr`T0!? ?be`T0rb|errtprepkrUe`T0`T0mr|rb|rbÆ0r`T0bb`T0bbŕ0`T0`T0B)0`T0`T0by0bb%0b` 0bQ` 0#0Kb0m.b "b0 mLatitude LongitudeSI03P1P2P3P4P5P6P7P8P9P10P11P12P13P14P15P16P17P18P19P20P21P22P23P24P25P35P26Vancouver Islandd.longd.latQueen Charlotte Islands Kodiak Isl  I&C&"Arial,Bold"&14 2001-08MHP DeskJet 895C Series Printer: d,,HP DeskJet 895C Series PrinterLPT1 ,,"d,,??3` 4#` 4#` 4#` 4#` 4# ` 4# ` 4#  2 (  ?}0 ~  <  @AA? : ]   $<Vancouver Island<l~~  <\ @AA?v ^ 3]\<  $<Queen Charlotte Islands<`$!f^   c $@T ]` f^  @ c $@ e  ]`~~  < @AA?7 b ]4 $<P4<Log~~  <L @AA? 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June 12, 20011   ##   ----'--- 9 F ---'--- 9 F ----'--- 9 F   2  50 2  51 2  52 2  53 2  54 2  55---'--- 9 F ---'--- 9 F   2 & 127 2 &B 129 2 &131 2 &-133 2 &135 2 &137 2 &139---'--- 9 F ------'--- ` 2 lLongitude (W)  '----'--- 9 F -----'---  =   Arial- 2 H Latitude (N)- ----'--- 9 F ---'--- 9 F - - 45- "-e - --- - K j Arial- -  2 SalpsdS!SK' 9 F --  - ' 9 F  '  'CompObj!fObjInfo#WorkbookkSummaryInformation($      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~ A\p Frank Whitney Ba=<="D<X@"1ZArial1ZArial1ZArial1ZArial1 ZArial MT1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial1ZArial"$"#,##0_);\("$"#,##0\)!"$"#,##0_);[Red]\("$"#,##0\)""$"#,##0.00_);\("$"#,##0.00\)'""$"#,##0.00_);[Red]\("$"#,##0.00\)7*2_("$"* #,##0_);_("$"* \(#,##0\);_("$"* "-"_);_(@_).))_(* #,##0_);_(* \(#,##0\);_(* "-"_);_(@_)?,:_("$"* #,##0.00_);_("$"* \(#,##0.00\);_("$"* "-"??_);_(@_)6+1_(* #,##0.00_);_(* \(#,##0.00\);_(* "-"??_);_(@_)"$"#,##0;\-"$"#,##0"$"#,##0;[Red]\-"$"#,##0"$"#,##0.00;\-"$"#,##0.00#"$"#,##0.00;[Red]\-"$"#,##0.0050_-"$"* #,##0_-;\-"$"* #,##0_-;_-"$"* "-"_-;_-@_-,'_-* #,##0_-;\-* #,##0_-;_-* "-"_-;_-@_-=8_-"$"* #,##0.00_-;\-"$"* #,##0.00_-;_-"$"* "-"??_-;_-@_-4/_-* #,##0.00_-;\-* #,##0.00_-;_-* "-"??_-;_-@_-0.0 0.0_) 0.000                       # # # #  @ "P "T  D # " " "T "T "    "  H     "\   " "T " 1" 1"T  1"\ 1"\ "\  "\ " "T   1" " !\  "   L  "\  "  "\  " 1!\ " & 83ffff̙3f3fff3f3f33333f33333\` X leg1 leg2 Acoast8Chart2 rsalps tracktrack 2  ;ZR3  @@   Ustationdegmindepthd.longd.latETAtravelStnArgoloopssurfC'coCTDno.saloxynutchlTCOAlkpHPC/NDOCPPFeZnTMb'gob'go2NstnBnscommentsm to nexttimefloatRosJPROSsam.FWLMTPKJDCSCDMh3Leg 1, Eddies. 14.5 d allotted (May 29 to June 13)SI3 May 29, 1200x'Rivers Inlet Stations (time permitting)RI 1 May 30, 1900btmRI 22000RI 32100RI 42200omit if time is shortRI 52300 Cape Scott/Triangle Island line:CS10 May 31, 0300arrive by 0300 hCS9CS8CS7CS6CS5CS4CS3CS2CS1>2000 may 31, 2200x?To eddy, loops every 4 hSampling depths:Rosette stations - 0, 10, 20, 30, 50, 75, 100, 125, 150, 175, 200, 250, 300, 400, 500, 600, 800, 1000 m (one duplicate depth per cast)Chl sampling to 75 m (6 depths)+Alk+DOC(m)Eddy survey 1 - Haida-2001ED1 Jun 1, 1500ED2ED3ED4ED5ED6ED7ED8ED9ED10ED11 Jun 3, 2000ED08 Jun 4, 0100 edge stationED12 Jun 5, 0600ED13ED14ED15 Jun 5, 1200center?ED15W Jun 6, 0900ED161200Bi'nsED171530ED18 Jun 6, 1930ED19 Jun 7, 0800outlv Jun 8, 0700Eddy survey 2 - Haida 2000ED20 Jun 8, 1500ED211900ED22ED23 Jun 9, 0200ED240500ED250800ED26 Jun 10, 0800ED271100edge?ED28 Jun 11, 0800ED291130ED30Argo6 Jun 12, 0330MassetJun 13, 0800-1800Eddy stations - 0, 10, 20, 30, 50, 75, 100, 125, 150, 175, 200, 250, 300, 400, 500, 600, 800, 1000, 1250, 1500, 1750, 2000 m (one duplicate depth per cast)cocco13CDMS5mCSMA/WRUBC3Leg 2, 15 d allotted (Jun 13, 1800 to Jun 28, 1800)Masset to Argo7Argo7 Jun 14, 0200Argo81330Z3 Jun 15, 0100Z2flt 1175flt 1128Line PP26 Jun 16, 0000P35 Jun 18, 0300P250700P24P231700P22P21 Jun 19, 0300P20P19 Jun 20, 0800P181300P171800P16P15 Jun 21, 2300P14 Jun 22, 0400P130900P121400P11 Jun 23, 1200P101500P09P08P072400P06 Jun 24, 0300P050600P04P03 Jun 25, 1200P02P01JF4JF3JF2 Jun 26, 0100JF10300offload at IOS Jun 28, 1800total est. time (d)Line P std. depths - 0, 10, 25, 50, 75, 100, 150, 200, 300, 400, 600, 800, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, 3000, 3500, 4000, btm-20 m (one duplicate when depth allows)*Robert and Whitney Coastline interpolationVancouver Island symbol tagsQueen Charlotte Islands Kodiak IslandPrince of Wales Island$Baranof and Chicagof (Sitka) IslandsKupreanof IslandAdmiralty IslandCoast Bristol BayBowie Seamount 1500 m line!Davidson Seamount, 1500 m line Hodgkins Seamount, 1500 m lineOshawa Seamount, 1500 m lineCobb Smt, 1500 fm ȵJ<rl_arBA8 ?Aok׻8 C(689|I_z80ϝY@ l@%^@NDD<% OH@DD< -4 ,8@,@  *333 5~ (@ 5 5 5(33$ $6N-,,*33333$ 7I@@_@ƭ@%5_@DD<%aWI@DD< 68 ">@"?  +44 9~ ,@ 5 !5 "5~ 4@&4$ :I@h@_@@@%p= _@DD<%4I@ DD< .; ">@"?  + ~  4?4 90!"44$ < I@@_@@ % ޖ:_@ D D <% S㥛I@ D D < .= ">@"? + ~ 4? 4 90 !"44$ > I@b@_@Ĥ@ % 1Z_@ D D <% h I@ D D < .? 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