ARS | Publication request: Comparative Mapping of Rpl3, a Gene over-Expressed in Multiple Obesity Models

Submitted to: Animal Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 1, 2001
Publication Date: July 31, 2001
Publisher's URL: http://www.dekker.com
Citation: Allan, M.F., Potts, J.K., Pomp, D. 2001. Comparative mapping of RP3, a gene over-expressed in multiple obesity models. Animal Biotechnology. 12(2):167-171.

Interpretive Summary: The ribosomal protein 3 gene is differentially expressed in hypothalamus and brown adipose tissue between mouse lines divergently selected for heat loss, and in skeletal muscle of the ob/ob mouse model. Unfortunately, multiple Rpl3-processed pseudogenes have hampered mapping of the functional gene copy in mammalian species. Using PCR amplification with intronic primer binding, we have mapped Rpl3 to MMU15, and have also localized RPL3 to BTA5 in cattle. Comparative mapping implicates a previously mapped copy of RPL3 on HSA22 as the functional copy of human RPL3, while predictive mapping places the porcine homologue on SSC5.

Technical Abstract: The ribosomal protein 3 gene is differentially expressed in the hypothalamus and brown adipose tissue between mouse lines divergently selected for heat loss and in skeletal muscle of the ob/ob mouse model. Unfortunately, multiple Rpl3-processed pseudogenes have hampered mapping of the functional gene copy in mammalian species. Using PCR amplification with intronic primer binding, we have mapped Rpl3 to MMU15, and have also localized RPL3 to BTA5 in cattle. Comparative mapping implicates a previously mapped copy of RPL3 on HSA22 as the functional copy of human RPL3, while predictive mapping places the porcine homologue on SSC5.