Bellavia A, Fraziano M, Mattei M, Poccia F, Caroleo MC, Salerno A, Moras L, Colizzi V; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: 13 (abstract no. PuA 6012).
Ist. Pat. Gen., Univ. Palermo.
OBJECTIVE: Since MRL mice produce antibodies against the gp120 V3 loop, and since similarities between Graft versus Host (GvH) disease and AIDS have been noted by several observers, we have developed a GvH disease in Severe Combined Immunodeficiency Disease (SCID) mice by transferring cells from MRL donors, as model for investigate the pathogenesis of AIDS. METHODS: GvH disease was induced in SCID mice by the intravenous injection of different doses of spleen or lymph nodes obtained from MRL at different age. As the majority of cells from MRL mice carry the CD4-CD8- (double negative, DN) phenotype and the SCID recipients lack mature T and B cells it was possible to follow MRL differentiation and activation by flow cytometry. Moreover, the cytotoxic activity against H-2d (SCID genotype), H-2k (MRL genotype) and H-2b (irrelevant genotype) was evaluated in a classical 51-Cr-release assay using spleen cells from Scid mice transferred with MRL cells. RESULTS: DN cells from MRL induce a lethal GvH disease when injected into SCID recipients, and the time of onset of GVH was related to the number of cells transferred and the age of MRL donors. This reaction is carried out by CD8+ MRL cells which differentiate in the SCID environment in 4-5 days. Spleen cells from SCID mice transferred with MRL cells expert a strong cytotoxic activity against H-2d but not against H-2b target cells. Moreover, an autocytotoxic activity was also observed using H-2k targets. CONCLUSIONS: Cells from MRL mice, which produce anti-HIV antibodies, are able to induce a lethal GvH disease when transferred into SCID recipients. Both differentiation of DN cells and development of allo-autocytotoxicity is observed into SCID. This GvH model may be helpful in the investigation of the AIDS pathogenesis and in the control of the related immunodeficiency.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- B-Lymphocytes
- Graft vs Host Disease
- Graft vs Host Reaction
- Immunologic Deficiency Syndromes
- Lymph Nodes
- Lymphocytes
- Mice
- Mice, Inbred BALB C
- Mice, Mutant Strains
- Mice, SCID
- Severe Combined Immunodeficiency
- Spleen
- immunology
Other ID:
UI: 102201261
From Meeting Abstracts