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Contents:
- Fitc and Rhodamine replacements
- Alternative non-UV nuclear stains
- Microscope slides and coverslips for direct cell culture growing
- Glass-bottom petridishes and culture wells for high n.a. oil immersion objectives
- LysoTracker
- CellTracker
- Mounting media with antifade reagents
FITC and Rhodamine replacements: Alexa Dyes
This new family of fluorescent dyes was specifically developed for laser lines available on confocal system, has much better signal and less bleaching than FITC. Available conjugated with secondary AB or as protein labeling kits.
Order from: Molecular Probes:
http://www.probes.com/lit/bioprobes26/section1.html
Alternative non-UV Nuclear Stains
TO-PRO 3 is a far red nucleic acid stain. Although almost invisible to the human eye, it is well suited for confocal studies and digital imaging with CCD cameras. It's far red emmission spectrum ensures minimum cross talk with GFP and other blue excitation dyes. Similary easy to use as DAPI or Hoechst.
No UV light source or laser required. Can be used on Zeiss LSM 410 confocal system
Order from:
Molecular Probes:
http://www.probes.com/lit/cellbio/section2.html
NUNC LabTek™ Chamber slides and chambered coverslips
Branch users: Kakali Sarkar (Shaw Lab)
Grow cell cultures directly on a microscope slide or on a coverslip, with up to 16 removable cell culture chambers on it.
For technical details and order information:
Nalgene NUNC:
http://nunc.nalgenunc.com/products/catalog/applications/flouresc.html
Glass bottom petridishes and culture wells
Branch users: Jie Zhu (Nussenzweig lab)
Petridishes for use with high n.a. oil immerson objectives on inverted microscopes. Available in 35mm and 50 mm diameters with coated or uncoated glass cover slips in the bottom.
Order from:
MatTek Corporation
Kathleen Goodhue
200 Homer Ave
Ashland, MA 01721
Phone: 800-634-9018
Fax: 508-879-1532
http://www.mattek.com/
WillCo Wells BV
WG plein 287
1054 SE Amsterdam
The Netherlands.
Phone : +.31.(0).20.685.01.71
Fax : +.31.(0).20.685.03.33
Http://www.willcowells.com
96 well culture plates with glass bottom are available from Polyfiltronics or Phenix Reserach Products. Long delivery times may be a problem:
| Polyfiltronics, Inc. 136 Weymouth St. Rockland, MA 02370-1136 USA Toll Free: 1-800-434-7659 Voice: 1-781-878-1133 Fax: 1-781-878-0822 Email: polyfil@polyfiltronics.com |
Phenix Research Products 3540 Arden Road Hayward, CA 94545 800-767-0665 Item# M77260039 |
LysoTracker
Branch users: Marta Catalfamo (Henkart lab)
Fluorescent probe that is selectively taken up by acidic organelles, particularly Lysosomes.
Order from:
Molecular Probes
http://www.probes.com/lit/cellbio/section6.html
CellTracker CMFDA
Branch users: Kirsi Allison-Ampe (Henkart Lab)
Fluorecescent probe staining living cells. Intracellular esterases activate fluorescence. Dye is trapped in cytoplasma and labels cells over several cell cycles.
Order from:
Molecular Probes:
http://www.probes.com/handbook/ch15-2.html
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Mounting media with antifade reagents
Prolong Antifade Kit
This mounting medium from Molecular Probes hardens within a few hours or overnight and has very good antifade properties. Prolong has to be prepared fresh right before use otherwise the antifade component degrades and can lead to very high autofluorescence.
Ordering info: Molecular Probes (catalog #P-7481)
Polyvinyl alcohol with DABCO
This is a mixture of Polyvinyl alcohol (PVA)and glycerol with
the antifade reagent DABCO (1,4-diazabicyclo[2.2.2]octane). PVA polymerizes on
contact with air and forms a seal around the the coverslip. (pH
8.7; n20D (refr. index): 1.381).
You can make a similar mounting media yourself. See the
protocols page for details.
Ordering info: Fluka (catalog #10981)
Vectashield
A ready to use mounting medium on an aqueous base, which does not harden. Vecat shield is a product of Vector Laboratoriess and comes also in variations with DAPI or Propidium Iodide as nuclear counterstains for FISH applications.
Ordering info: Vector Laboratories (catalog # H1000)
Fluoromount G
A ready to use mounting medium for aqueous specimen supplied by several companied, e.g. Southern Biotechnology Associates, Inc., Electron Microscopy Sciences
Ordering info:
Southern
Biotechnology (Catalog #0100)
Electron
Microscopy Science (Catalog#17984-25)
Permount
Resin-based mounting medium for dehydrated specimen. Permount does not contain a dedicated antifade reagent.
Ordering info:
Fisher
Scientific (Catalog#S70104)
Electron
Microscopy Science (Catalog #17986-01)
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