|
|
Contents:
FITC and Rhodamine replacements: Alexa DyesThis new family of fluorescent dyes was specifically developed for laser lines available on confocal system, has much better signal and less bleaching than FITC. Available conjugated with secondary AB or as protein labeling kits. Order from: Molecular Probes: Alternative non-UV Nuclear StainsTO-PRO 3 is a far red nucleic acid stain. Although almost invisible to the human eye, it is well suited for confocal studies and digital imaging with CCD cameras. It's far red emmission spectrum ensures minimum cross talk with GFP and other blue excitation dyes. Similary easy to use as DAPI or Hoechst. No UV light source or laser required. Can be used on Zeiss LSM 410 confocal system Order from: Molecular Probes: NUNC LabTek™ Chamber slides and chambered coverslipsBranch users: Kakali Sarkar (Shaw Lab)Grow cell cultures directly on a microscope slide or on a coverslip, with up to 16 removable cell culture chambers on it. For technical details and order information:
Glass bottom petridishes and culture wellsBranch users: Jie Zhu (Nussenzweig lab)
Petridishes for use with high n.a. oil immerson objectives on inverted microscopes. Available in 35mm and 50 mm diameters with coated or uncoated glass cover slips in the bottom. Order from: MatTek Corporation WillCo Wells BV 96 well culture plates with glass bottom are available from Polyfiltronics or Phenix Reserach Products. Long delivery times may be a problem:
LysoTrackerBranch users: Marta Catalfamo (Henkart lab)
Fluorescent probe that is selectively taken up by acidic organelles, particularly Lysosomes. Order from: Molecular Probes CellTracker CMFDABranch users: Kirsi Allison-Ampe (Henkart Lab)
Fluorecescent probe staining living cells. Intracellular esterases activate fluorescence. Dye is trapped in cytoplasma and labels cells over several cell cycles. Order from: Molecular Probes: Mounting media with antifade reagentsProlong Antifade KitThis mounting medium from Molecular Probes hardens within a few hours or overnight and has very good antifade properties. Prolong has to be prepared fresh right before use otherwise the antifade component degrades and can lead to very high autofluorescence. Ordering info: Molecular Probes (catalog #P-7481) Polyvinyl alcohol with DABCOThis is a mixture of Polyvinyl alcohol (PVA)and glycerol with
the antifade reagent DABCO (1,4-diazabicyclo[2.2.2]octane). PVA polymerizes on
contact with air and forms a seal around the the coverslip. (pH
8.7; n20D (refr. index): 1.381). Ordering info: Fluka (catalog #10981) VectashieldA ready to use mounting medium on an aqueous base, which does not harden. Vecat shield is a product of Vector Laboratoriess and comes also in variations with DAPI or Propidium Iodide as nuclear counterstains for FISH applications. Ordering info: Vector Laboratories (catalog # H1000) Fluoromount GA ready to use mounting medium for aqueous specimen supplied by several companied, e.g. Southern Biotechnology Associates, Inc., Electron Microscopy Sciences Ordering info: PermountResin-based mounting medium for dehydrated specimen. Permount does not contain a dedicated antifade reagent. Ordering info:
|
|
National
Institutes of Health|National Cancer
Institute|
Division of Basic Science|Experimental
Immunology Branch |