Antisera

Colleagues: I do send limited amounts of antisera to people doing original scientific research. Please check the cross-reactivity panel for antisera at (http://csdb.nidcr.nih.gov/csdb/frame_reagents.htm) to see if your choice of antisera is appropriate for your model system (although the list is not always complete so I will sometimes make changes or additions to your requests). Generally I do not send more than one antiserum against any one protein unless there is a clear reason for such duplicate requests. Unless otherwise noted, all antisera are whole rabbit serum. All antisera are made in limited amounts and therefore some may no longer be available. Most antisera are whole rabbit serum antisera but there are a limited number of monoclonals. At this time, I usually cannot send monoclonal antibodies to non-USA destinations due to high handling costs.

However, Santa Cruz Biotechnologies Inc. is now carrying our most commonly requested SIBLING monoclonals.

BSP (LFMb-24)sc-73634mouse IgG1131-442 (h)WBh, monkey
BSP (LFMb-25)sc-73630mouse IgG1257-276 (h)WB, IF, IHC(P)h, monkey, cow
DMP-1 (LFMb-31)sc-73633mouse IgG1internal (h)WB, IF, IHC(P)h, monkey
DSP (LFMb-21)sc-73632mouse IgG1487-502 (h)WB, IF, IHC(P)h, monkey
OPN (LFMb-14)sc-73631mouse IgG2b173-186 (h)WB, IF, IHC(P)h, monkey, mou., rab, rat, sheep
MEPE (LFMb-33)sc-73635mouse IgG1244-262 (h)WB, IF, IHC(P)h, monkey

For most monoclonals, animal species other than human, monkey & mouse have not been tested.

To comply with the National Institutes of Health’s requirements I ask that you complete (in duplicate!) the Simple Letter Agreement (SLA) with Research Plan.

Please follow the instructions on the last page of the agreement. Your brief research project must include the model species (mouse, cow etc.) so that I can send the most appropriate antisera/cDNA. Send the completed duplicate SLA forms to me at: Larry W. Fisher, Ph.D. Matrix Biochemistry Unit, CSDB, DIR NATIONAL INSTITUTES OF HEALTH/NIDCR BUILDING 30 ROOM 228 BETHESDA MD 20892-4320. I will obtain approval and send the Research Materials. I reserve the right to limit the number probes sent (particularly in the case of duplicate requests of antisera for detecting the same protein) and to substitute in cases where antisera are limited or I think that a different probe would be more effective. Please remember that our web site is under construction. My apologies for any errors or confusion.

For colleagues in COMMERCIAL laboratories, you MUST contact the Technology Development Coordinator here at the NIDCR about the necessity of obtaining a license for the probes prior to contacting the person mentioned at the web site. Questions about legal or technical aspects of the MTA should be directed to the Technology Development Coordinator.

The NIDCR intramural technology transfer office handles intramural requests.

Material Transfer Agreements should be filled out according to these instructions.

Upon receipt of the signed MTA from NIH, I will ship by regular airmail. For requests from within the USA, inclusion of your FedEx number (and phone number) may shorten the shipping times.

Sincerely,

Larry W. Fisher, PhD
Chief, Matrix Biochemistry Section
Craniofacial and Skeletal Diseases Branch
NIDCR/NIH

The list of Antisera are shown in the following tables:


Proteoglycan polyclonal antisera

Gene product

Antiserum Name

Antigen source

Known species

Comments

References
Human decorin LF-136 GIGPEVPDDRDF-[KLH] Hum & Mon only increased signal strength with pretreatment with ABC'ase Fisher et al. 1989b

Bianco et al. 1990
Human decorin propeptide LF-110 QVSWAGPFQQRGLFDC-[LPH] Hum, others not determined but seq no cross to BGN propeptide Fisher et al.
1995
Human decorin propeptide LF-111 QVSWAGPFQQRGLFDC-[CSA] (amino acids 5-17) Hum, others not determined but seq is highly conserved no cross to BGN propeptide Fisher et al.
1995
Human decorin LF-122 recombinant protein including propeptide Hum, Mon, Pig, She, Chi, cow(?)

 

Fisher et al.
1995
Human biglycan LF-51 GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera Hum & Mon only increased signal strength with pretreatment with ABC'ase Fisher et al. 1989b

Bianco et al. 1990
Human biglycan LF-112 GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera Hum & Mon only increased signal strength with pretreatment with ABC'ase Fisher et al. 1989b

Bianco et al. 1990
Human biglycan propeptide LF-104 LPFEQRGFWDFTLDDC-[LPH] Hum, Mon, Rat, Mou

 

Fisher et al.
1995
Human biglycan propeptide LF-105 LPFEQRGFWDFTLDDC-[CSA] others not determined

 

Fisher et al.
1995
Human veriscan LF-99 LHKVGKSPPVRC-[LPH] only human tested

 

Bernstein et al. 1995b
Human veriscan LF-98 LHKVGKSPPVRC-[CSA]

 

 

 

Human biglycan LF-121 recombinant protein including propeptide Hum & Mon only

 

Fisher et al.
1995
Bovine decorin LF-94 IGPEEHFPEVPEC-[LPH] only cow tested Cow domain analogous to Human LF-30 Fisher et al.
1995
Bovine decorin LF-95 IGPEEHFPEVPEC-[CSA] for these 2 antisera Cow domain analogous to Human LF-30 Fisher et al.
1995
Bovine biglycan LF-96 LPDLDSLPPTYSC-[LPH] only cow tested Cow domain analogous to Human LF-15 series Fisher et al.
1995
Bovine biglycan LF-97 LPDLDSLPPTYSC-[CSA] for these 2 antisera Cow domain analogous to Human LF-15 series Fisher et al.
1995
Murine decorin LF-113 IIPYDPDNPLISMC-[LPH] only tested for Mouse domain analogous to Human LF-30 series Fisher et al.
1995
Murine decorin LF-114 IIPYDPDNPLISMC-[CSA] Mou and Rat Mouse domain analogous to Human LF-30 series Fisher et al.
1995
Murine biglycan LF-106 VPDLDSVTPTFSAMS-[LPH] only tested for Mouse domain analogous to Human LF-15 series Fisher et al.
1995
Murine biglycan LF-107 VPDLDSVTPTFSAMS-[CSA] Mou and Rat Mouse domain analogous to Human LF-15 series Fisher et al.
1995
Murine biglycan LF-159 VPDLDSVTPTFSAMS-(LPH) Mou and Rat Mouse domain analogous to Human LF-15 series  
Murine fibromodulin LF-149 CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH Mouse, others not tested histology requires digestion with chondrointinase ABC  
Murine fibromodulin LF-150 CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH Mouse, others not tested histology requires digestion with chondrointinase ABC, same as LF-149  

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Sialoprotein polyclonal antisera
Gene Product

Antiserum name

Antigen source

Known species cross-reactivity

Comments

References


Human osteopontin		 LF-19 IPVKQADSGE homopolymer Hum & Mon only

 

Fisher et al. 1989b


Human osteopontin		 LF-86 TVDTYDGRGDSVVYGLR
SLS-[LPH]		 Hum, Mon human osteopontin RGD domain unknown utility Fisher et al.
1995


Human osteopontin		 LF-161 CLYNKYPDAVATWLNPDPSQKQNLLAPN conjugated to KLH only human tested human exon 4  


Human osteopontin		 LF-166 recomb. Carboxyl half starts at thrombin site Hum, Mon, Pig, Cow, She, Rat, Mou does not contain the RGD domain Fisher et al. 1995 based onOP-10 plasmid (Young et al. 1990a)


Human osteopontin		 LF-124 recombinant amino half ending at thrombin site Hum, Mon, Cow, She, Rat, Mou contains the RGD domain Fisher et al. 1995 based on OP-10 plasmid (Young et al. 1990a)


Mouse OPN		 LF-175 recombinant mouse OPN (E coli) Mou, Rat OK for WB, IF, IHC(P)  


Human osteopontin		 LFMb-14 Monoclonal*, IgG2b human osteopontin, likely epitope is within KFRRPDIQYPDATD All tested to date: Hum, Mou, Rat, Sheep, Rabbit, Monkey Mouse monoclonal (IgG) works in westerns and paraffin sections  


Human bone sialoprotein		 LFMb-24 Monoclonal*, IgG1 recombinant (AA131-442) Hum, Monkey May be slightly more sensitive on WB than LFMb-25 Bellahcène A, Castronovo V, Ogbureke KU, Fisher LW, Fedarko NS. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.


Human bone sialoprotein		 LFMb-25 Monoclonal*, IgG1 recombinant (AA131-442), epitope probably within: VAYEGEYEYTGANGYDNGYE Hum, Monkey OK for WB, IF & IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.


Human bone sialoprotein		 LF-83 YESENGEPRGDNYRAYE

D-[LPH]		 Hum, Mon, Dog epitope appears to be RAYED Bianco et al. 1993

Mintz et al. 1993


Human bone sialoprotein		 LF-84 YESENGEPRGDNYRAYE

D-[LPH]		 Rat, Mou, Chi   Fisher et al.
1995


Human bone sialoprotein		 LF-100 AIQLPKKAGDIC-[LPH] Hum, Mon, Dog, Rat mid-protein antigen Mintz et al. 1993


Human bone sialoprotein		 LF-101 AIQLPKKAGDIC-[CSA] Hum, Mon, Dog, Rat, Pig, Cow mid-protein antigen Fisher et al.
1995


Human bone sialoprotein		 LF-119 recombinant RGD domain (AA257-317) Hum, Mon, Dog, Chi mid-protein antigen similar to LF-100 Fisher et al.
1995 based on B6-5g plasmid (Fisher et al. 1990)


Human bone sialoprotein		 LF-120 recombinant Frag 1 (aa 129-281) Hum, Mon, Pig, Dog, Rat

 

Fisher et al.
1995 based on B6-5g plasmid (Fisher et al. 1990)


Rat (UMR) bone sialoprotein		 LF-87 rat BSP isolated from UMR-106-BSP media Rat, Mou, Hom full sulfated form of rat BSP Fisher et al.
1995

Bianco et al. 1993


Rat (UMR) bone sialoprotein		 LF-90 rat UMR-106 BSP grown in chlorate Rat Low sulfate(<5%) sulfate 0n BSP Fisher et al.
1995


Human dentin matrix protein-1 (DMP1)		 LFMb-31 recombinant exon 5 (last), epitiope probably within: SESQEEVVSESRGDNPDPTTSYVE Hum, Monkey OK for WB (but DMP1 is difficult to capture in standard WB) IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.


Human dentin matrix protein 1 (DMP1)		 LF-148 CEHPSRKIFRKSRISE and CLKNIEIESRKLTVDAYH conjugated to KLH human and bovine, others not tested    


Human dentin matrix protein 1 (DMP1)		 LF-160 CGSDDHQYIYRLAGGFSRSTGKGGDD & CRDTGLSQPRRDSKGDSQED conjugated to KLH human and bovine, others not tested    


Human DSP (of DSPP)		 LFMb-21 Monoclonal*, IgG1 synthetic peptide: KGQEGQDHGKEDDHDS Hum, Monkey OK for WB, IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.


Hum dentin sialophosphoprotein (DSPP)		 LF-151 part of DSP portion of human DSPP, bacterial recombinant human and bovine, not mouse    


Hum dentin sialophosphoprotein (DSPP)		 LF-154 non repetitive portion of last exon, human DSPP human and mouse, others not tested appears to recognize DSP portion of last exon  


Mou dentin sialophosphoprotein (DSPP)		 LF-153 part of DSP portion of mouse DSPP, bacterial recombinant mouse, not human or cow    


Human MEPE		 LFMb-31 Monoclona*, IgG1 recombinant last exon, epitope within THRIQHNIDYLKHLSKVKKI Hum, Monkey OK for WB, IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.


Human MEPE		 LF-155 last exon of human MEPE made in bacteria human, others not tested    


Human MEPE		 LF-158 CSIYPKSTGNKGFEDGDD & CSTHRIQHNIDYLKHLSKVKK conjugated to KHH human, others not tested weaker titer than LF-155 on ELISA  

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Collagen polyclonal antisera
Gene product

Antiserum Name

Antigen source

Known Species Cross-reactivity

Comments

References
Bovine a1 (I) amino-propepetide LF-9 bovine bone N-propeptide Hum, Mon, Pig, She

 

Fisher et al. 1987b
Human a1 (I) amino-propepetide LF-39 EEGQVEGQDEDIPPITCVQ-[KLH] Hum, Cow, She

 

Fisher et al. 1989a
Human a1 (I) amino-propepetide LF-40 EEGQVEGQDEDIPPITCVQ-[KLH] Hum, Cow, She

 

Fisher et al. 1989a
Human a1 (I) carboxy-propept. LF-41 PLDVGAPDQEFGFDVGPVCFL-[KLH] All species except, perhaps Rab detects proal(1) and C-propeptide Fisher et al. 1989a
Human a1 (I) carboxy-propept. LF-42 PLDVGAPDQEFGFDVGPVCFL-[KLH]     Fisher et al.
1995
Human a1 (I) carboxy-telopept. LF-67 SAGFDFSFLPQPPQEKAHDGGRYYRA All species except, detects proal(1) Bernstein et al. 1995b
Human a1 (I) carboxy-telopept. LF-68 SAGFDFSFLPQPPQEKAHDGGRYYRA perhaps Rab pCal(1), a(1) + / - reduction Fisher et al.
1995
Human a2 (I) amino-telopeptide LF-116 QYDGKGVGLGPGPC-[LPH] Hum, not mouse, others not tested

 

 

Human type III collagen C-propeptide LF-69 DIGGPDQEFGVDVGPVCFL Hum, Mon, others not tested seq. homology to type II but not tested. Reduction increases reaction Bernstein et al. 1995b
Human type III collagen C-propeptide LF-70 DIGGPDQEFGVDVGPVCFL Hum, Mon, others not tested seq. homology to type II but not tested. Reduction increases reaction Fisher et al.
1995
Human type III collagen N-telopeptide LF-71 EYDSYDVKSGVAC-[KLH] Hum, Mon,

 

Bernstein et al. 1995b
Human type III collagen N-telopeptide LF-72 EYDSYDVKSGVAC-[KLH] others not tested

 

Fisher et al.
1995

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Miscellaneous polyclonal antisera

Gene product

Antiserum Name

Antigen Source

Known species cross-reactivity

Comments

References
Human osteonectin LF-37 EALPDETEVVEETVAEVT
EVP-[KLH] (AA 4-25)		 Hum, Mon Also known as SPARC Fisher et al.
1995
Bovine osteonectin BON-I

BON-II		 bovine bone osteonectin (both) All species tested Also known as SPARC Ingram et al. 1993
Bovine osteonectin LF-55

LF-56		 first 56 AA of mature Cow sequence (both) only Cow and Hum tested Also known as SPARC Fisher et al.
1995
Bovine osteonectin LF-53

LF-54		 AA 27-56 of mature Cow sequence (both) only Cow and Hum tested Also known as SPARC Fisher et al.
1995
Chicken osteonectin LF-8 chicken bone osteonectin Chi, Quail Also known as SPARC Fisher et al.
1995
Chicken osteonectin LF-45 APQEALPDETEVIED-[KLH] Chi, Quail AA 1-15 of mature Fisher et al.
1995
Mouse osteonectin LF-23

LF-24		 TEVAEEIVE EET-[KLH] (both) (AA 5-16 of mature) Mou, Rat, She, Chi Also known as SPARC Wewer et al. 1988
Bovine osteocalcin LF-32 bovine bone osteocalcin Cow, Hum, Mon also known as bone gla protein (BGP) Ingram et al. 1993
Bovine alkaline phosphatase LF-52 from bovine kidney affinity purified by Sigma unknown bone-liver-kidney form of the enzyme Fisher et al.
1995
Human alkaline phosphatase LF-47

LF-48		 LVPEKEKDPKYWRDQAQ
ETC-[KLH] (both) (AA 1-19)		 Hum (weak), Cow, Pig, Rab human sequence but weak activity Tulli et al. 1992
Human osteoinductive factor (OIF) LF-102

LF-103		 CLKRLPIGSYF-(LPH)

CLKRLPIGSYF-(CSA)		 both not tested C-term of OIF, both uncharacterized Fisher et al.
1995


Human MMP-3		 LF-182 synthetic peptide: EPGFPKQIAEDFPGIDSKIDAC Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW. SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 2007 Apr;55(4):403-9. Epub 2007 Jan 8.


Human MMP-2		 LF-183 synthetic peptide: ENQSLKSVKFGSIKSDWLGC Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW. SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 2007 Apr;55(4):403-9. Epub 2007 Jan 8.


Human MMP-9		 LF-184 synthetic peptide: RSELNQVDQVGYVTYDILQCPED Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW. SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 2007 Apr;55(4):403-9. Epub 2007 Jan 8.


Mouse MMP-14		 LF-176 catalytic domain synthetic peptide: CPLRFREVPYAYIREGHEKQAD Hum, Mouse IHC, WB  


Mouse MMP-14		 LF-177 hemopex domain synthetic peptide: CRTTSRPSVPDKPKNPAYG Hum, Mouse IHC(P), WB  


Mouse MMP-14		 LF-178 combined peptides from LF-176 & LF-177 Hum, Mouse IHC(P), WB