Improved enzyme-linked immunosorbent assay to detect didecyldimethylammonium chloride, a quaternary ammonium compound.

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Title:	Improved enzyme-linked immunosorbent assay to detect didecyldimethylammonium chloride, a quaternary ammonium compound.
Author:	Bull, J.P. : Serreqi, A.N. : Gamboa, H.R. : Breuil, C.
Citation:	J-agric-food-chem. Washington, D.C. : American Chemical Society. Nov 1998. v. 46 (11) p. 4779-4786.
Abstract:	Didecyldimethylammonium chloride (DDAC) is a commonly used wood protectant in Canada and must be monitored due to environmental concerns. Three new DDAC-like immunogens were synthesized and tested for their ability to produce antibodies that complex DDAC. The polyclonal antisera from rabbits immunized with the new hapten conjugates were compared by hapten homologous indirect competitive enzyme-linked immunosorbent assay (CELISA). The resulting antisera recognized DDAC to different degrees. CELISA based on anti-DDAC 3, 2, and 1 antisera had IC50S of 0.05, 16.0, and 17.2 micrograms/mL, respectively. All the antisera were more sensitive to DDAC than a previously reported polyclonal antiserum. The most sensitive and selective antiserum was produced from the hapten that most closely mimicked DDAC. The selectivity of the three immunoassays for compounds representing DDAC's different epitopes was tested. Each immunoassay exhibited the highest selectivity with compounds that most closely resembled the hapten. The effect of solvents and detergents was determined for the most sensitive immunoassay: CELISA based on anti-DDAC 3 antiserum. Recovery experiments were also carried out with this assay. This immunoassay (IC50 50 ppb) holds promise for the analysis of environmental samples since the regulatory discharge limit is 700 ppb.
Review References:	None
Notes:	Includes references.
Language:	English
Publication Type:	Article
Keywords:	None
URL:	http://pubs.acs.org/journals/jafcau/index.html