MEILAND R, GEERLINGS SE, BROUWER EC, COENJAERTS FE, LANGERMANN S, HOEPELMAN IM; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. L-1349.
University Medical Center Utrecht, Utrecht, The Netherlands
BACKGROUND: Women with DM have a higher prevalence of bacteriuria than women without DM. We have shown that this is due to an increased adherence of type 1-fimbriated E. coli to uroepithelial cells of women with DM. A FimCH-based vaccine is being clinically evaluated. METHODS: Uroepithelial cells were isolated from urine of women with DM. E. coli strains: Ctr 39(pyelonephritis isolate expressing type 1 fimbriae) and NU14 (well characterized, highly adherent cystitis isolate expressing type 1 fimbriae). FITC-labeled bacteria were incubated with anti-FimCH antiserum in 3 dilutions, or buffer(PBS/glycerol/BSA). Then, the bacteria were washed and incubated with uroepithelial cells. Mean fluorescence/cell was calculated by FACS-analysis. RESULTS: The adherence of E. coli to uroepithelial cells of women with DM was inhibited by 65%(SEM 7.4), 56%(15.6), and 41%(26.9) for Ctr 39 and by 23%(12.9), 8%(9.8), and 3%(6.8) for NU14, after incubation with anti-FimCH antiserum 1:50, 1:100 and 1:200, respectively. [figure: see text] CONCLUSION: 1. Anti-FimCH antiserum inhibits the adherence of type 1-fimbriated E. coli to uroepithelial cells of women with DM; 2. A clinical study to evaluate the vaccine in women with DM is warranted.
Publication Types:
Keywords:
- Adhesins, Escherichia coli
- Bacteriuria
- Cystitis
- Diabetes Mellitus
- Escherichia coli
- Escherichia coli Infections
- Female
- Humans
- Immune Sera
- Pyelonephritis
- Vaccines
- immunology
- urine
Other ID:
UI: 102270621
From Meeting Abstracts