Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Inhibition of atrial natriuretic peptide-induced cyclic GMP accumulation in the bovine endothelial cells with anti-atrial natriuretic peptide receptor antiserum |
| Creator/Author | Ishido, M. ; Fujita, T. ; Shimonaka, M. ; Saheki, T. ; Ohuchi, S. ; Kume, T. ; Ishigaki, I. ; Hirose, S. |
| Publication Date | 1989 Jan 05 |
| OSTI Identifier | OSTI ID: 6397714 |
| Other Number(s) | CODEN: JBCHA |
| Resource Type | Journal Article |
| Resource Relation | J. Biol. Chem. ; Vol/Issue: 264:1 |
| Research Org | Tokyo Institute of Technology (Japan) |
| Subject | 550201 -- Biochemistry-- Tracer Techniques; CYCLASES-- ENZYME ACTIVITY;GUANYLIC ACID-- BIOLOGICAL ACCUMULATION;PEPTIDES-- RECEPTORS; BIOCHEMICAL REACTION KINETICS;CAROTID ARTERIES;CATTLE;CELL CULTURES;ENDOTHELIUM;IMMUNOASSAY;INHIBITION;IODINE 125;TRACER TECHNIQUES |
| Related Subject | ANIMAL TISSUES;ANIMALS;ARTERIES;BETA DECAY RADIOISOTOPES;BLOOD VESSELS;BODY;CARDIOVASCULAR SYSTEM;DAYS LIVING RADIOISOTOPES;DOMESTIC ANIMALS;ELECTRON CAPTURE RADIOISOTOPES;ENZYMES;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;KINETICS;LYASES;MAMMALS;MEMBRANE PROTEINS;NUCLEI;NUCLEOTIDES;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANS;PROTEINS;RADIOISOTOPES;REACTION KINETICS;RUMINANTS;TISSUES;VERTEBRATES |
| Description/Abstract | Using an antiserum raised against the purified atrial natriuretic peptide (ANP) receptor that has a disulfide-linked homodimeric structure and represents one subtype of the multiple ANP receptors, we showed that the receptor is coupled to the guanylate cyclase activation; formerly, this type of ANP receptor is not considered to be coupled to the cyclase.^The specificity of the antiserum was determined by immunoblot analysis and immunoprecipitation.^The anti-receptor antiserum did not compete with /sup 125/I-ANP for binding to the receptor but it lowered the affinity of the receptor.^When added to bovine endothelial cell cultures, the antiserum blocked the cyclic GMP response of the cells triggered by ANP.^These results indicate that the subtype of the ANP receptor recognized by the antiserum is responsible for the activation of particulate guanylate cyclase as well as the double function type receptor that has been assumed to contain both the receptor domain and the catalytic domain for cGMP synthesis on the same molecule.^The presence of dissociative complexes of ANP receptor and particulate guanylate cyclase was also demonstrated by radiation inactivation analysis. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: 641-645 |
| System Entry Date | 2001 May 13 |
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Last Updated: 02/11/2009