Rabanus JP, Greenspan D, Petersen V, Greenspan JS; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 341 (abstract no. 1110).
University of California, School of Dentistry, Oral AIDS Center, San Francisco, California, USA
OBJECTIVE: The observation that a rabbit antiserum against common papilloma-virus (PV) antigen reacts with the nuclei of keratinocytes in oral hairy leukoplakia (HL) has been difficult to reconcile with data showing heavy infection with EBV but no other evidence for PV. The objective of this study was to locate the binding sites of the antiserum at the ultrastructural level. METHODS: Three specimens of clinically suspected HL and two specimens of oral warts were fixed in 4% paraformaldehyde, sucrose-infiltrated, and shock-frozen in slush nitrogen. Ultrathin sections were incubated with MAb against components of EBV, MAb against cellular proteins, and with rabbit antiserum against the common PV antigen. The sections were labeled with immunogold conjugates and examined by electron microscopy. RESULTS: Antiserum against PV strongly reacted with arrays of intranuclear particles of about 35 nm, which are commonly seen in EBV-producing keratinocytes in HL. The antiserum also reacted with intranuclear structures of keratinocytes in oral warts. None of the MAb reacted with the intranuclear arrays in HL. CONCLUSION: Staining of the nuclei of keratinocytes in HL with anti-PV antiserum is due to reaction with arrays of intranuclear particles, the nature and role of which remain to be established.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Antigens, Viral
- Cell Nucleus
- Herpesvirus 4, Human
- Immune Sera
- Keratinocytes
- Leukoplakia, Hairy
- Microscopy, Electron
- Papilloma
- Papillomaviridae
- Rabbits
- immunology
Other ID:
UI: 102196963
From Meeting Abstracts