Isoprenylcysteine carboxyl methyltransferase RNA [primary]
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Experiment Id: 6822
Pattern Id: GC16224
Clone number: 346878
GenBank Acc:
W78108
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Rationale: Relative RNA levels for nearly 10,000 human clones were measured in a microarray experiment. Some of these clones have been linked to known genes (based on clustering information from Unigene) - follow the links to Unigene and GeneCards, where information on possible function can be found. Other clones represent as yet unnamed genes - follow the Unigene link for more information. All gene assignments should be considered tentative. Sequence information for a clone can be found through GenBank or Unigene, and can be used to search sequence databases.
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Investigator(s):
Botstein, Dr. David
Stanford University School of Medicine
Weinstein, Dr. John
National Cancer Institute
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Method:
Microarray
Unit:
log(mRNA levels in cell line/mRNA levels in reference pool)
Protocol:
mRNA was isolated from logarithmically growing cells. Labelled cDNA was prepared by reverse transcription of test cell mRNA in the presence of Cy5-dUTP. Reference probes were made by pooling equal amounts of mRNA from HL-60, K562, NCI-H226, COLO205, SNB-19, LOX-IMVI, OVCAR-3, OVCAR-4, CAKI-1, PC-3, MCF7 and Hs578T cell lines. Labelled cDNA was prepared from the pooled mRNA by reverse transcription in the presence of Cy3-dUTP. Test and reference probes were combined, denatured and hybridized overnight to Synteni microarrays containing cDNA from 9703 human clones. Arrays were scanned using a laser-scanning microscope. The ScanAlyze program was used to analyze the microarray images and prepare data files for further analysis.
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Target Data:
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Compare:
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Genecard
A database of human genes, their products and their involvement in diseases.
It offers concise information about the functions of human genes.
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Unigene
An experimental system for automatically partitioning GenBank sequences into a non-redundant
set of gene-oriented clusters.
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Entrez Gene
Presents information on official nomenclature, aliases, sequence accession numbers, phenotypes,
EC numbers, MIM numbers, UniGene clusters, map information, Gene Ontology annotations,
and relevant web sites. Replaces LocusLink.
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OMIM
A catalog of human genes and genetic disorders.
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PubMed
Search PubMed for articles on:
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CGAP
CGAP provides information on gene expression, SNPs, functional pathways and
more in normal, precancerous and cancer cells.
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Email questions concerning DTP's molecular targets program to:
Susan Holbeck [holbecks@mail.nih.gov]
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