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Title Posttranslational modification of the Ha-ras oncogene protein: Evidence for a third class of protein carboxyl methyltransferases
Creator/Author Clarke, S. ; Vogel, J.P. ; Deschenes, R.J. ; Stock, J. (Princeton Univ., NJ (USA))
Publication Date1988 Jul 01
OSTI IdentifierOSTI ID: 5482401
Other Number(s)ISSN0027-8424; CODEN: PNASA
Resource TypeJournal Article
Resource RelationProceedings of the National Academy of Sciences of the United States of America ; Vol/Issue: 85:13
Subject550201 -- Biochemistry-- Tracer Techniques; METHYL TRANSFERASES-- BIOCHEMISTRY;PROTEINS-- POST-TRANSLATION MODIFICATION; CYSTEINE;ELECTROPHORESIS;EMBRYONIC CELLS;FIBROBLASTS;METHIONINE;METHYLATION;ONCOGENES;RATS;SULFUR 35;TRITIUM COMPOUNDS
Related SubjectAMINO ACIDS;ANIMAL CELLS;ANIMALS;BETA DECAY RADIOISOTOPES;BETA-MINUS DECAY RADIOISOTOPES;CARBOXYLIC ACIDS;CHEMICAL REACTIONS;CHEMISTRY;CONNECTIVE TISSUE CELLS;DAYS LIVING RADIOISOTOPES;DRUGS;ENZYMES;EVEN-ODD NUCLEI;GENES;HYDROGEN COMPOUNDS;ISOTOPES;LIGHT NUCLEI;LIPOTROPIC FACTORS;MAMMALS;NUCLEI;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC SULFUR COMPOUNDS;RADIOISOTOPES;RODENTS;SOMATIC CELLS;SULFUR ISOTOPES;THIOLS;TRANSFERASES;VERTEBRATES
Description/Abstract The ras oncogene products require membrane localization for their function, and this is thought to be accomplished by the addition of a palmitoyl group to a cysteine residue near the carboxyl terminus of the nascent chain.^A lipidated carboxyl-terminal cysteine residue is also found in sequence-related yeast sex factors, and in at least two cases, the {alpha}-carboxyl group is also methyl esterified.^To determine if ras proteins are themselves modified by a similar type of methylation reaction, the authors incubated rat embryo fibroblasts transformed with p53 and activated Ha-ras oncogenes with L-(methyl-{sup 3}H)methionine under conditions in which the isotope was converted to the methyl donor S-adenosyl-L-(methyl-{sup 3}H)methionine.^By using an assay that detects methyl ester linkages, they found that immunoprecipitated ras proteins are in fact esterified and that the stability of these esters is consistent with a carboxyl-terminal localization.^This methylation reaction may be important in regulating the interaction of ras proteins with plasma membrane components.^The presence of analogous carboxyl-terminal tetrapeptide sequences in other proteins may provide a general recognition sequence for lipidation and methylation modification reactions.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 4643-4647
System Entry Date2001 May 13

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