Goudsmit J, McKeating J, Meloen R, Smit L, Bakker M, Barin F; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 480 (abstract no. C.732).
Human Retrovirus Laboratory, Amsterdam, the Netherlands
OBJECTIVE: To localize HIV-2 external envelop domains binding neutralizing antibodies. METHODS: Five hundred overlapping peptides that covered the complete amino acid sequence of the external envelope of the HIV-2 ROD strain were synthesized with the antibody-reactive peptide scanning (PEPSCAN) technique. Sera of HIV-2 infected individuals were used as probe for antibody reactivity. Rabbits were immunized with KLH coupled peptides of domains reactive with human sera. Sera were tested for HIV-2 neutralization. RESULTS: Fifteen domains were identified that bound serum of HIV-2 infected individuals. The majority of the sera reacted with two regions: one in the amino terminal half (aa 108-132) and one in the carboxyl terminal half (aa 303-507) of the external envelope. Each of the human HIV-2 neutralizing sera reacted with several carboxyl terminal epitopes. Sera of rabbits immunized with peptides covering the extreme terminal part of the external envelope neutralized HIV-2. Cross-neutralization with HIV-1 of these sera is under investigation. CONCLUSION: Epitopes localized in the extreme terminal carboxyl part of the HIV-2 external envelope bind natural neutralizing antibodies and immunization of rabbits with these domains results in HIV-2 neutralizing capacity of their serum.
Publication Types:
Keywords:
- Amino Acid Sequence
- Animals
- Epitopes
- HIV-1
- HIV-2
- Humans
- Peptides
- Rabbits
- immunology
Other ID:
UI: 102179489
From Meeting Abstracts