CLUSTAL W (1.74) multiple sequence alignment yigN ------------------------------------------------------------ ZyigN ------------------------------------------------------------ VC0082 ------------------------------------------------------------ NMB2050_2 ------------------------------------------------------------ NMA0386 MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAARCDFVEQAHAEIASEL PA1031 ------------------------------------------------------------ HI0500 ------------------------------------------------------------ PM1267 ------------------------------------------------------------ CT825 ------------------------------------------------------------ CPn0982 ------------------------------------------------------------ CC0271 ------------------------------------------------------------ RP690 ------------------------------------------------------------ mlr4857 ------------------------------------------------------------ SPy0266 ------------------------------------------------------------ L185224 ------------------------------------------------------------ XF0413 ------------------------------------------------------------ yigN ------------------------------------------------------------ ZyigN ------------------------------------------------------------ VC0082 ----------------------------------------------------------MF NMB2050_2 ------------------------------------------------------------ NMA0386 AVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLKQSYIELQEKAQGLAVENE PA1031 ------------------------------------------------------------ HI0500 ------------------------------------------------------------ PM1267 -----------------MVNRMSELTTTQYLLIIAVLIISCVVLFFISARRQRDSQELQQ CT825 ------------------------------------------------------------ CPn0982 ------------------------------------------------------------ CC0271 ------------------------------------------------------------ RP690 ------------------------------------------------------------ mlr4857 ------------------------------------------------------------ SPy0266 ------------------------------------------------------------ L185224 ------------------------------------------------------------ XF0413 ------------------------------------------------------------ yigN ------MDFSIMVYAVIAL--VGVAIGWLFAS-YQHAQQKAEQLAERE---------EMV ZyigN ------MDFSIMVYAVIAL--VGVAIGWLFAS-YQHAQQKAEQLAERE---------EMV VC0082 NMQWIFENQSALWSALVSAGATGVAIGWWVKQRYQLKTQLLEQQLEQQSHWHAQQIQQLN NMB2050_2 ------------------------------------------------------------ NMA0386 RLATQLGQERKAFADQYAL--ERQIRQRIETD-LEESRQTVRDVQNDLA--------DVG PA1031 -----------MLGERLAT--AQLAQDGLNAQ-LETSREAYQQLGERHA--------RFA HI0500 ------------------------------------------------------------ PM1267 DLNKTIEDFNQLLSKFEAVQQAKNQLEQHVVK-YQTTAEGLQVRLIERDDKLAYLQKELD CT825 -----------------------------------------------------MGVFAIS CPn0982 ----------------------------------------------------------MN CC0271 --------------------------------------------------------MNFS RP690 -------------------------------------------------------MPSLL mlr4857 ---------------------------------------------------MPGHRIGYA SPy0266 --------------------------------------------------------MDLI L185224 --------------------------------------------------------MEVI XF0413 ------------------------------------------------------MQPDFL yigN AELSAAKQQITQSEHWR-------AECELLN--------------------------NEV ZyigN AELSAAKQQITQSEHWR-------AECELLN--------------------------NEV VC0082 EQLTTAQQELDELDALR--D---KNEFELKQSHGKLMAVLEKLRYFEAVKQERQQYADEL NMB2050_2 ---AAAEKQIAHLQE---K----EAEAERLR-------------------------QSHT NMA0386 NRFAAAEKQIAHLQE---K----EAEAERLR-------------------------QSHT PA1031 GEVAALRREAELMGEERQR----ASQAEARW-------------------------ASER HI0500 ------------------------------------------------------------ PM1267 EEQARHNDIAEKITALKERFGIASAQAESLAKQLEHSQAN------------FTRKEQEN CT825 LLSQTVCLYFTFFSLGI-------ALGVLFS-------------------------FKIF CPn0982 LPVSLACLLLSGCVF---------FLGVFVS-------------------------SSLY CC0271 DPYLILALVFALAAVALG------LWAVAAQ--------------------------RRA RP690 LLTTTILLILCVLIIWFY------IKTHTLK--------------------------RQL mlr4857 VHMNDLNTILSQPVARLG------ASTITLG-------------------------HALA SPy0266 LFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEG---------------------NADNLSD L185224 IIILLILLILLSLANFFKKTD-KGNTSHQLNYLNQNLSEMRSDINQQLAQNRQELSSSLR XF0413 ILGCLLCIVLVLQILALLRRSDHTALDHVLREEHRVGRAELREQLETLERQQEVRLASFA yigN RSLQSINTSLEADLREVTTRMEAAQQHADDKIRQMINSEQRLS----EQFENLANRIFEH ZyigN RSLQSINTSLEADLREVTTRMEAAQQHADDKIRQMINSEQRLS----EQFENLANRIFEH VC0082 NQVRAAKAELESQLREQEARHQQQLVASQEKLQLLERAEERLK----QQFEHLANQVFEH NMB2050_2 ELQEKAQG-LAVENERLATQIEQERLASEEKLSLLGEARKSLS----DQFQNLANTILEE NMA0386 ELQEKAQG-LAVENERLATQIEQERLASEEKLSLLGEARKSLS----DQFQNLANTILEE PA1031 QGREEELRRLASERAALAAELREQQESHQQRLTDLQSARDELR----AQFAELAGKIFDE HI0500 -----------------------------------------------MEFQNLANRILDE PM1267 QELMAKLTTLSQELTELKTTLSEKEKHFAEQQQHIEQSKQQLS----TEFQNLANRILDE CT825 TKKLSRQHEIIRDLEHSKAILQMSLDTRRSQEQLMEEFSHKLT----SVSQAFARDMKTE CPn0982 ARKKRAFLEKIQKLEHENQLLQTSLNLSRHQEQLIEDFSNRLA----LSSHKLIKDMKEE CC0271 AKAEAQAWLLTERLAQAEERHRLLEDQSATQIELIKAQAAQSA----NAIAEQLIKRADE RP690 QFLSEQNLEINNNNRLLNQEKIAYLQKIEQLKCKVEYQEQMIK----DSEKIREESFTSA mlr4857 FGALLFLGLFLALVVALWRSAKARAVAAAEAADHARDAEARMAGI--LQSQAEMQGRMGA SPy0266 QMTYQLDTANKQQLLELTQLMNRQQAGLYQQLTDIRDVLHRSL----SDSRDRSDKRLEK L185224 SVNNDFSALTENINSKFDWQFKDLQNSNEQKLEKISVSLVEST----DRTVSKLSELTES XF0413 RSLTDLVARIDQRLDQLTASLGEDARKGRQEASEGQQRFADALGQRLTELTQRNAQSINE yigN SNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSFG-KEAQERHTLTHEIRNLQQLNAQM ZyigN SNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSFG-KEAQERHTLTHEIRNLQQLNAQM VC0082 KTATVDVQNRQSLEGLLTPLKEQLEGFKKQVNDSFN-HEAKERHTLVHELRNLQRLNEQM NMB2050_2 KSRRFTEQNREQLHQVLNPLNERIHGFGELVKQTYD-KESRERLTLENELKRLQGLNAQL NMA0386 KSRRFTEQNREQLHQVLNPLNERIQGFGELVKQTYD-KESRERLTLENELKRLQGLNAQL PA1031 REQRFAETSQQRLGQMLDPLKERIQAFEKRVEESYQ-QEARERFSLSKELERLQQLNLRL HI0500 KSRSFSQSNQTALETLLKPFREQIEGFQKRVNEIHS-ESVKGNAGLEAEIKKVLEIGLNM PM1267 KSRSFSQSNQTALDSLLKPFREQIEGFQKRVNEIHS-ESVKGNAGLEAEIKKVLEIGLNM CT825 SQEFFSEKT-QAITSVLAPVHNTLSAFKQNLENFET-KQAEDRGALKEQLSQLLTAEQKL CPn0982 AQNYFGDTS-KSFQSILSPIQTTLTTFKQSLETFET-KHAEDRGRLKEQISQLLAVEKKL CC0271 NAKSRERLAEARLEAQLKPVAETLAKFEAQVTAVEK-ARAEETGGLKAQITALLEASTAT RP690 KAALFDLGKDLSKQLIEIHKIENNTARELAEQNITT-ASRKFNSELERLITMVGALNKDI mlr4857 IAEVFGARQAELTQSIGQRLDAMTGRIGQTMTEQTK-STHESLAKLQERLAIIDTAQGNI SPy0266 INQQVNQSLKNMQESNEKRLEKMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEM L185224 ITAKFDHKFKELQDANDKRLSQIQETVDEKLQETLNRRISQSFEQVTLHLKNVEEGLGEM XF0413 MRTTLEQQLHVLRADNAQQLEQIRAIVDEKLQTTLNTRLDSSFKLVSERLEQVQRGLGEM : : : yigN AQEAINLTRALKG-D-NKTQGNWGEVVLTRVLEASGLREGYEYETQVSIENDARS----R ZyigN AQEAINLTRALKG-D-NKTQGNWGEVVLTRVLEASGLREGYEYETQVSIENDARS----R VC0082 AKEAVNLTQALKG-D-NKQQGNWGEVVLARVLAESGLREGHEYQTQVSLQNEAGK----R NMB2050_2 HSEAKALTNALTGTQ-NKVQGNWGEMILETVLENSGLQKGREYVVQAASVRKEEDGGTRR NMA0386 HSEAKALTNALTGTQ-NKVQGNWGEMILETVLENSGLQKGREYVVQAASVRKEEDGGTRR PA1031 GEEATNLTRALKG---QKTQGNWGELVLERVLEHAGLEKGREYETQVSLKGAEGE----R HI0500 SQEASNLTSALKG-E-KKTLGNWGEVQLERALQLAGLEENVHYRAQAHFKDEQGG----R PM1267 SQQADNLTSALKG-E-KKTLGNWGEVQLERALQLAGLLEGEHYSAQAHLKDGEGK----H CT825 ERETQALTNILKH---PGSRGRWGEIQLERILEISGMLKYCDYSTQTVDSSESSS----- CPn0982 EHETHVLTDILKH---PGSRGRWGEIQLERILELAGMLKYCDYDSQTT-SAQGAF----- CC0271 QAEARKLSAALRR-G-AGVQGRWGEQTLRNVLEAAGLNSRFDFQEQFSVDSEEGR----R RP690 EQSKSTVDLIKQSLLSPIGAGLLSEITLENILKSSGLRPNLDFIMQYGLTTSDSV----K mlr4857 QSLAGQVVQLQAILSNKQTRGAFGQSRMEAIVAD-GLPHG-AYEFQAT--LSNGS----- SPy0266 RSVAQDVGTLNKVLSNTKTRGILGELQLGQIIED--IMTSSQYEREFVTVSGSSE----- L185224 RNLASDVDSLQKVMTGVKTRGIVGEIQLGRILEQ--MFTSSQYREQVNIQGGNAV----- XF0413 QQLATGVGDLKRVLTHVKSRGTWGEVQLDNILDQ--TLTQEQYARGVRVCPDASE----- : * .: : : : yigN MQPDVIVRLPQGK------DVVIDAKMTLVAYERYFNAEDDYTRESALQEHIASVRNHIR ZyigN MQPDVIVRLPQGK------DVVIDAKMTLVAYERYFNAEDDYTRESALQEHIASVRNHIR VC0082 YQPDVIVHLPQNK------QVVIDSKMALVAYERYFHAETDSERDSALREHLLALRNHIR NMB2050_2 LQPDVLVNLPDNK------QIVIDSKVSLTAYVRYTQAADADTAARELAAHVASIRAHMK NMA0386 LQPDVLVNLPDNK------QIVIDSKVSLTAYVRYTQAADADTAARELAAHVASIRAHMK PA1031 FQPDVLIRLPGDK------QVVVDAKVSLTAYQQYIAADDDLLRQQALKQHVTSLRNHVK HI0500 NYPDFVLNLPDEK------HLIIDSKMSLVAYESAVNSEDDFERERLLKEHISALKTHIN PM1267 NYPDFVLNLPDNK------HLIIDSKMSLVAYESAVNAEDDLKRQNKLREHIKAIKNHID CT825 -RADIVIRLPQNR------SLVIDAKTPFSEEYLTDNHADPTDLVKKIKDHIKTLK---- CPn0982 -RADIIIRLPQDR------CLIIDAKAPISDSYFSVEEIDKGDLVDKIKEHIKTLK---- CC0271 -RPDVKVTLPGGG------VFVIDAKCSLNAFLEAQDATDEVLREAAMARHAQSVRTHMQ RP690 LRPDAIIFLPSGN------LMVIDSKASKFLVDSQDNSVN---LSKTMNYHLKSLANKDY mlr4857 -RPDCLVKMPNGAP-----SLAIDAKFPLEAWNAIRAADGADLQKVASQAFRRDIEIHVR SPy0266 -RVEYAIKLPGNGQGGY-IYLPIDSKFPLEDYYRLEDAYEVG-DKLAIEASRKALLAAIK L185224 ---DYALILPGKSDDSE-LLLPIDSKFPMEDYQRLQMALESN-DSLEIDKTRKALFNAVK XF0413 -IVDFAVRLPGRGQYEAPVWLPIDVKCPKEDYERLLDAQEQA-DQELVRTMGVQLERAIK : : :* . :* * . : yigN LLG-RKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITEALKNNIMLVSPTTLLVA ZyigN LLG-RKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITEALKNNIMLVSPTTLLVA VC0082 GLG-QKDYHQLKGIQSLDYVLMFIPVEPAFQVAIQADPSLVNDAMEQNIILVSPTTLLVA NMB2050_2 GLS-LKDYTDLEGVNTLDFVFMFIPVEPAYLLALQNDAGLFQECFDKRIMLVGPSTLLAT NMA0386 GLS-LKDYTDLEGVNTLDFVFMFIPVEPAYLLALQNDAGLFQECFDKRIMLVGPSTLLAT PA1031 GLS-GKDYRRLEGLHSLDFVLLFMPIEAAFSTALQAEPNLFQDAFAQNIVIVSPTTLLAT HI0500 DLH-KKDYSNLIGMCSPNFVLMFIAVEPAYIEALKADPALFNYGYERNVIMVSHTTLMPI PM1267 DLS-RKDYSNLIGMRSPNFVLMFIAVEPAYIEAMKMDPTLFNYGYEKNVILVSHTTLMPI CT825 -T--KSYWDKFE--QSPEFVILFLPGESLFNDAIRCAPELMDYAGQSNVILSSPVTLMAL CPn0982 -S--KSYWEKFH--QSPEYVILFLPGESLFNDAIRLAPELMEIGASSNVILSSPLTLLAL CC0271 GLSSKAYWDQFAGEGSPDFVAMFVPGDGFLAAALDRLPELMAEAMDRRVVLVTPTTLFAL RP690 AENILTTLNKKAHNFNNVITLMFLPTEQAVEKVIAANPEFLQKAWGCNIFPVGPAGLMNM mlr4857 DIS-EKYLIQGE---TQDTAFMFVPSESVFAEIHENFEAIVHKAHRARVVIVSPSLLMLS SPy0266 RFAKDIHKKYLNPPETTNFGVMFLPTEGLYSEVVRNASFFDSLRREENIVVAGPSTLSAL L185224 AQAKSISEKYIVPPKTTDFAIMFLPTEGLFMEVVNNPELYEQISRDYKVNVTGPTTMTAV XF0413 IQAKSIRDKYIVPPHTTDFAVMFLPTEGLYAETIRRPGLADVLQREYRVVVAGPTTVTAL . :*:. : .: : yigN LRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAIGQSLDKAQDNYRQAMKKLS ZyigN LRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAIGQSLDKAQDNYRQAMKKLS VC0082 LRTIDNLWRNERQNQNAQLIAERASKLYDKLRLFVEDMEGLGGALDKANQSYQGAMNKLV NMB2050_2 LRTVANIWRNEQQNQNALAIADEGGKLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLA NMA0386 LRTVANIWRNEQQNQNALAIADEGGKLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLA PA1031 LRVIDSLWRQERQNQNAREIAERAGSLYDKFVLFVQDLDEVGSRLQQLDKAYAAARNKLT HI0500 LRTVANLWRIERGNAEAKEIAEKAGEIYNQICLVAERLNKLGNTLSTVSNQYNSTVTALV PM1267 LRTVANLWRIERGNAEAKEISARAGDIYNQICVIAERLGKLGNTLSSASSHYNSTVTALV CT825 LKTVTHVWKQENLQNQIREIGQLGKDLYQRMHKLFDHFHKVGKHLGQAVHSYNDMSSSLS CPn0982 LKTIAYMWKQENLQKQIQEVSLLGKELHRRLQVVFTHFQKIGKNLNQTVQSYNDMTSSFQ CC0271 CKAVAYGWRAEDQAKNAARIVEVGRELYKRVSVMGAHAGAMGKALESAVGKYNQFVGSLE RP690 LSFAKFQITDNRRSENYKVIISEVRKLLSSIGTIADYSKKIGYNLQNMVTNYDKFAASFN mlr4857 IQVIQAILKDARMREQAHLIQGEVIRLMEDVARVDERVRKLQVHFGQSAKDIDDILVSTS SPy0266 LNSLSVGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRT L185224 LNSLQMGFKTLQIEQKSAEVYELLGAVKTEFGKFEGSLKKVQERLQQSEKEISNLITTRT XF0413 LNSLQMGFRTLAIEQRSSEVWSLLGAVKSEFGKFAGILEKAEKQINTVGKSLGEAG-RKT . : : . . : yigN SGRGNVLAQAEAFRGLGVEIKREIN-PDLAEQAVSQDEEYRLRSVPEQPNDEAYQRDDEY ZyigN SGRGNVLAQAEAFRGLGVEIKREIN-PDLAEQAVSQDEEYRLRSVPEQPNDEAYQRDDEY VC0082 TGRGNAIRQAESFKQLGVEIKRSIP-QPLIERAQQAPDQENAPLVERHP------IEDKV NMB2050_2 EGRGNLVGRAEKLRLLGVKAGKQLQ-RDLVERSNET--TALSESLEYAA-------EDEA NMA0386 EGRGNLVGRAEKLRLLGVKANKQLA-RDLTERANET--TALSESLEYVA-------EDEA PA1031 EGRGNLISRSEQLKLLGARASKSLP-TDWLDR-------ALVAPVEEAG-------EE-- HI0500 GQQG-LVGKVERFKTLSAKANKTMPDVDLLNNQLDL---ARLNVLNQENLQ--------- PM1267 GQQG-LVGKVERFKDLSAKANKTMPNVELLHHAVES---ERLDVIKADTPEAQPQKDNTA CT825 ARVLPILRTFDKLELSSSHNKIEALSQVSTLPHSPKVPYPENDLAECLSPEASYLKPPSS CPn0982 YRVLPTLRKFEGLETSSSH-QIEEPTPIESLATS--FPHT-CDIDTNLAVIESLEKQD-- CC0271 SQVLTQARRFEDLSVDHEG--KEIPEPPMVDQAVRP--LTKLKAVGQSPTLTLGNDTPT- RP690 RNLMSRVKTIQKLGIDSGD--KAMPATLERYQIVSS-KSEIIEVDAENPT----QIEE-- mlr4857 KVTK-RGQKIEALEFGAQADGDAGSETPSRAPAAKLEPGPRVADSKTGQLRLRVVEGDD- SPy0266 NAIVRALNTVETYQDQATKSLLNMPLLEEENNEN-------------------------- L185224 NVMNRKLRNINEIDNSKSSELLGIDNE--------------------------------- XF0413 RTIERRLRGVESLSQEQTQVLLDGLDVDAAESDVDSDASF-------------------- : yigN NQQSR ZyigN NQQSR VC0082 N---- NMB2050_2 V---- NMA0386 V---- PA1031 ----- HI0500 ----- PM1267 Q---- CT825 NQ--- CPn0982 ----- CC0271 ----- RP690 ----- mlr4857 ----- SPy0266 ----- L185224 ----- XF0413 -----