Speaker: Aiqun Li, Protein Chemistry Laboratory, SAIC-Frederick, Frederick, MD 21701

Topic: Chemical Cleavage of Apomyoglobin: Search for an Alternative to Enzymatic Digestion for Gel-Isolated Protein Samples

Place: Building 426, Conference Room, NCI-Frederick, Frederick, MD

Time: Tuesday, January 9, 2001, at 2:00 PM

Abstract: Peptide mass mapping by enzymatic digestion has become a routine method for protein characterization and identification. However, enzymatic digestion is usually time consuming and the spectra are complicated by protease autolysis products. We have found chemical cleavage at aspartic acid a promising alternative to enzymatic digestion.