2006 Annual Report 4d.Progress report. This report serves to document research conducted under a SCA between ARS and the University of Illinois at Urbana-Champaign. Additional details of research can be found in the report of the parent project, 1935-41000-070-00D, Enzyme-based Technologies for Milling Grains and Producing Biobased Products and Fuels. This accomplishment aligns with National Program 306 Component 2, New Processes, New Uses, and Value-Added Biobased Products Problem Area 2c, "New and Improved Processes and Feedstocks." This research project was developed with the University of Illinois at Urbana-Champaign to pursue the common goal of conducting a commercial plant scale trial of the jointly developed enzymatic wet milling process. A commercial plant trial of the Enzymatic Wet Milling process was conducted at a 200 metric ton per day facility in Malaysia during fiscal year 2006 by researchers from the Crop Conversion Science and Engineering research unit in cooperation with researchers from the University of Illinois under a SCS, 1935-4100-070-05S, Enzymatic Milling Commercial Plant Trial. Previous trials had been done in small batch operations and could not be used to demonstrate the effectiveness using a continuous system. Modifications were made and a series of conventional runs were conducted prior to switching the facility to run using the enzymatic process. Five separate enzymatic runs were conducted and yields and coproducts collected for analysis from the conventional and enzymatic runs. Starch yields were found to be significantly higher using the enzymatic process when compared to the conventional starch yields and coproducts were not found to be significantly different in composition. This successful demonstration will allow other wet milling facilities to better evaluate the enzymatic wet milling technology and determine the economic advantages relative to the current process. The patented process is currently being licensed by a major enzyme manufacturer.