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BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= SSH4C06.SEQ(1>505) (474 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 505,245 sequences; 158,518,215 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 6 Sequences     : less than 6 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 1318 334 |=======================================================
   6310  984 211 |===================================
   3980  773 175 |=============================
   2510  598 189 |===============================
   1580  409 142 |=======================
   1000  267  98 |================
    631  169  47 |=======
    398  122  43 |=======
    251   79  22 |===
    158   57  11 |=
    100   46  14 |==
   63.1   32  10 |=
   39.8   22  14 |==
   25.1    8   1 |:
   15.8    7   2 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 5  <<<<<<<<<<<<<<<<<
   10.0    5   1 |:
   6.31    4   1 |:
   3.98    3   2 |:
   2.51    1   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|1174318|gb|AAB35482.1|Ca2+/calmodulin-dependent pr... -1    65  0.80      1
gi|7189444|gb|AAF38354.1|(AE002212) hypothetical prot... -3    41  0.93      2
gi|7482503|pir||F69124hypothetical protein MTH201 - M... -1    76  0.98      1
gi|3915439|sp|O13695|YEN1_SCHPOHYPOTHETICAL 52.9 KD S... -1    83  0.996     1
gi|1174316|gb|AAB35480.1|Ca2+/calmodulin-dependent pr... -1    67  0.999     1



Locally-aligned regions (HSPs) with respect to query sequence:

Locus_ID                Frame -1 Hits
gi|1174318             |                                                  
gi|7482503             |                                                  
gi|3915439             |                                                  
gi|1174316             |                                                  
                        __________________________________________________
Query sequence:        |               |               |               |  | 158
                       0              50             100             150


Locus_ID                Frame -3 Hits
gi|7189444             |           ___           ____                     
Prosite Hits:                        __                                   
                        __________________________________________________
Query sequence:        |               |               |               |  | 158
                       0              50             100             150
__________________
Prosite hits:
   CYTOCHROME_C         Cytochrome c family heme-binding site si 43..48
__________________

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Related >gi|1174318|gb|AAB35482.1|  Ca2+/calmodulin-dependent protein kinase beta e
            isoform, beta e CaM kinase {alternatively spliced} [rats,
            Sprague-Dawley, Peptide Partial, 74 aa]
            Length = 74

Frame -1 hits (HSPs):   ______________________________________________    
                        __________________________________________________
Database sequence:     |            |             |            |          | 74
                       0           20            40           60

  Minus Strand HSPs:

 Score = 65 (22.9 bits), Expect = 1.6, P = 0.80
 Identities = 21/69 (30%), Positives = 29/69 (42%), Frame = -1

Query:   234 ASLSSVLAEINSRLRNVVGNMQGDNTVPSGQMESNSRDLSSGSESRPPTVNKQQDTVDVN 55
             A L+++LA  N      + N + D   P      NS  ++S   S PP   + Q TV  N
Sbjct:     1 AILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPPAALEPQTTVIHN 60

Query:    54 GFGAISASS 28
                 I  SS
Sbjct:    61 PVDGIKESS 69


to_Entrezto_Relatedto_Related >gi|7189444|gb|AAF38354.1|  (AE002212) hypothetical protein [Chlamydophila
            pneumoniae AR39]
            Length = 39

Frame -3 hits (HSPs):      _______________            ___________         
                        __________________________________________________
Database sequence:     |                        |                         | 39
                       0                       20

  Minus Strand HSPs:

 Score = 41 (14.4 bits), Expect = 2.6, Sum P(2) = 0.93
 Identities = 5/11 (45%), Positives = 8/11 (72%), Frame = -3

Query:   274 WVWYFYLTATF 242
             W W+FYL + +
Sbjct:     4 WQWFFYLYSQY 14

 Score = 37 (13.0 bits), Expect = 2.6, Sum P(2) = 0.93
 Identities = 6/8 (75%), Positives = 7/8 (87%), Frame = -3

Query:   133 KQQGLVFW 110
             K+QGL FW
Sbjct:    25 KKQGLCFW 32


to_Entrezto_Relatedto_Related >gi|7482503|pir||F69124  hypothetical protein MTH201 - Methanobacterium
            thermoautotrophicum (strain Delta H) >gi|2621246|gb|AAB84707.1|
            (AE000807) unknown [Methanobacterium thermoautotrophicum]
            Length = 147

Frame -1 hits (HSPs):                   __________________________        
                        __________________________________________________
Database sequence:     |                |                |                | 147
                       0               50              100

  Minus Strand HSPs:

 Score = 76 (26.8 bits), Expect = 3.7, P = 0.98
 Identities = 19/75 (25%), Positives = 35/75 (46%), Frame = -1

Query:   324 TIPSHPPGVGVSSSTQTGFGISTSQPPS---DSASLSSVLAEINSRLRNVVGNMQGDNTV 154
             T  S  P    S  + TG G  TS+ PS   ++ S     A+++++ +  +    G+ + 
Sbjct:    49 TTTSPAPTKKTSDRSSTGSGTKTSKSPSGGVETGSAQGEAADVSAQNKTDIQGQDGNEST 108

Query:   153 PSGQMESNSRDLSSG 109
             PSG +    +  +SG
Sbjct:   109 PSGNITGKEKQGTSG 123


to_Entrezto_Relatedto_Related >gi|3915439|sp|O13695|YEN1_SCHPO  HYPOTHETICAL 52.9 KD SERINE-RICH PROTEIN
            C11G7.01 IN CHROMOSOME I >gi|7492035|pir||T37544 hypothetical
            serine-rich protein - fission yeast (Schizosaccharomyces pombe)
            >gi|2408006|emb|CAB16206.1| (Z99161) hypothetical serine-rich
            protein. [Schizosaccharomyces pombe]
            Length = 536

Frame -1 hits (HSPs):      ___________                                    
Annotated Domains:      __________________________________________________
                        __________________________________________________
Database sequence:     |             |             |             |        | 536
                       0           150           300           450
__________________

Annotated Domains:
   Entrez               Domain: SER-RICH.                        25..238
   Entrez               Transmembrane region: POTENTIAL.         247..267
   Entrez               Transmembrane region: POTENTIAL.         351..371
   PRODOM               PD000651: VL2(74) O18758(34) MUC2(10)    3..228
   PRODOM               PD126628: YEN1_SCHPO                     230..535
__________________


  Minus Strand HSPs:

 Score = 83 (29.2 bits), Expect = 5.5, P = 1.0
 Identities = 29/111 (26%), Positives = 53/111 (47%), Frame = -1

Query:   339 MS*QQTIPSHPPGVGVSSSTQTGFGISTSQPPSDSASLSSVLAEINSRLRNVVGNMQGDN 160
             +S    IPS    + +SSS+ T    S+S  P  S+S +S  +  +S + ++V +    +
Sbjct:    35 LSSSSVIPSSSSSM-LSSSSATAISSSSSSSPLSSSSFTSPAS--SSFITSLVSSSSQQS 91

Query:   159 TVPSGQMESNSRDLSSGSESRPPTVNKQQDTVDVNGFGAI-SASSVGCTSES 7
             +  S  + S+S    + S S  PT +     +  +    + S+SS G +S S
Sbjct:    92 SSSSASLTSSSSATLTSSSSASPTSSSSSHALSSSSSSLVASSSSSGMSSSS 143


to_Entrezto_Related >gi|1174316|gb|AAB35480.1|  Ca2+/calmodulin-dependent protein kinase beta
            isoform, beta CaM kinase {alternatively spliced} [rats,
            Sprague-Dawley, Peptide Partial, 98 aa]
            Length = 98

Frame -1 hits (HSPs):          ________________________________________   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 98
                       0        20        40         60        80

  Minus Strand HSPs:

 Score = 67 (23.6 bits), Expect = 6.8, P = 1.0
 Identities = 21/80 (26%), Positives = 34/80 (42%), Frame = -1

Query:   267 GISTSQPPSDSASLSSVLAEINSRLRNVVGNMQGDNTVPSGQMESNSRDLSSGSESRPPT 88
             G  T+ P + S + S     +  + ++++ N + D   P      NS  ++S   S PP 
Sbjct:    15 GRQTTAPATMSTAASGTTMGLVEQAKSLL-NKKADGVKPQTNSTKNSSAITSPKGSLPPA 73

Query:    87 VNKQQDTVDVNGFGAISASS 28
               + Q TV  N    I  SS
Sbjct:    74 ALEPQTTVIHNPVDGIKESS 93


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.98

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.363   0.164   0.612  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.329   0.145   0.473  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.365   0.160   0.617  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.317   0.131   0.387    same    same    same
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.346   0.152   0.530  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.364   0.161   0.701  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      157       157       10.  74 3  12 22  0.098   34
                                                    30  0.12    36
   +2      0      157       157       10.  74 3  12 22  0.098   34
                                                    30  0.12    36
   +1      0      158       157       10.  74 3  12 22  0.098   34
                                                    30  0.12    36
   -1      0      158       158       10.  74 3  12 22  0.095   34
                                                    30  0.097   37
   -2      0      157       157       10.  74 3  12 22  0.098   34
                                                    30  0.12    36
   -3      0      157       157       10.  74 3  12 22  0.098   34
                                                    30  0.12    36


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  8:50 PM CDT May 27, 2000
    Format:  BLAST
  # of letters in database:  158,518,215
  # of sequences in database:  505,245
  # of database sequences satisfying E:  5
  No. of states in DFA:  593 (58 KB)
  Total size of DFA:  195 KB (256 KB)
  Time to generate neighborhood:  0.02u 0.00s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  4
  Search cpu time:  216.28u 1.26s 217.54t  Elapsed: 00:01:35
  Total cpu time:  216.33u 1.28s 217.61t  Elapsed: 00:01:36
  Start:  Wed Feb 14 17:55:17 2001   End:  Wed Feb 14 17:56:53 2001

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000