Kossovsky N, Sponsler E, Gelman A, Rajguru S, Mena E, Festekjian A, Pham M, Hnatyzn J; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 37 (abstract no. WS-A21-3).
UCLA Medical Center 90024-1732.
Immunostimulatory complexes based on lipids such as QuilA have been shown to evoke anti HIV immunity in animal systems. A non-lipoidal system is presented. We describe the synthesis of vaccinating viral decoy particles consisting of ceramic carbon nanocrystalline carriers, a polysaccharide film, and an outer coat of adsorbed HIV membrane proteins. Physical, in vitro biological and in vivo assays show that the HIV decoys have remarkably similar properties to live virus. At physiological pH the mean electrophoretic mobility and average dispersion diameter (50 nm) of these viral decoys closely mimiced that of their infectious counterparts. Immunoagglutination and immunogold labeling of the decoy HIV using monoclonal conformationally sensitive anti-HIV membrane antibodies and binding assays to CD4+HELA cells suggest that the protein's native conformational integrity was preserved. Vaccination of mouse, guinea pig, and rabbit elicit the production of antisera which exhibit specific binding to whole HIV preparation as measured by ELISA and Western blots. Binding affinity to HIV was actually higher in rabbits vaccinated with decoy. Histological analysis of live viral dermal challenge sites for animals sensitized to decoy virus and whole virus showed similar (qualitative and quantitative) reactions. HIV-ISCOMS exhibit an immunogenic profile remarkably similar to live HIV. Non-lipoidal ISCOMS may to be a promising vaccination strategy for AIDS.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- HIV Infections
- HIV Seropositivity
- Humans
- ISCOMs
- In Vitro
- Mice
- Rabbits
- Simian Acquired Immunodeficiency Syndrome
Other ID:
UI: 102204331
From Meeting Abstracts