Broor S, Kausari A, Zhang B, Seth P, Richman DD, Carson DA; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 170 (abstract no. PO-A13-0214).
AIIMS, New Delhi, India.
This study examined the effects of leukemia inhibitory factor (LIF) on HIV replication in mononuclear phagocytes (MNP). LIF induced a dose-dependent increase in p24 antigen production in the chronically infected promonocytic cell line U1. The magnitude and time kinetics of the LIF effects were similar to IL-1, IL-6 and TNF. Some of the mechanisms responsible for this LIF effect were also studied. LIF increased levels of HIV mRNA which was detectable by 24 hours and persisted until 72 hours. NF-kB levels were higher in unstimulated U1 cells than the parent cell line U937. Induction of NF-kB and HIV replication by cytokines are in part dependent on reactive oxygen intermediates. N-acetyl-L-cysteine inhibited LIF induced HIV replication. The effect of LIF on HIV replication were not inhibited by antibodies to IL-1, TNF or IL-6. These results identify LIF as a stimulus of HIV replication.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Cell Line
- Cytokines
- Growth Inhibitors
- HIV
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- Interleukin-1
- Interleukin-6
- Monocytes
- NF-kappa B
- Phagocytes
- immunology
- methods
Other ID:
UI: 102203023
From Meeting Abstracts