Gupta P, Balachandran R, Thampatty P, Rinaldo C; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 537 (abstract no. Th.C.O.28).
University of Pittsburgh, Pittsburgh, PA 15261, USA
OBJECTIVE: To compare in vitro infectivity, replication properties and genomic structure of random and longitudinally obtained HIV isolates from asymptomatic men and AIDS patients. METHODS: HIV isolates were examined for their in vitro infectivity of CD4+ H9 cells, and DNA and RNA synthesis by hybridization with an HIV probe. Viral protein synthesis was measured by the radioimmunoprecipitation. The viral genomic structure was compared by restriction endonuclease analysis of high M.W. cellular DNA. RESULTS: Most of the HIV isolates from asymptomatic men, but not from AIDS patients, failed to infect CD4+ H9 cells and PHA-stimulated PBL. However, in asymptomatic HIV seroconverters who developed AIDS, longitudinal HIV isolates at all stages of infection could infect H9 cells; in contrast, longitudinal isolates from men who remained asymptomatic did not infect H9 cells. HIV isolates from AIDS patients exhibited increased production of virus particles and intracellular viral DNA, RNA and protein as compared with isolates from asymptomatic men. Isolates from asymptomatic men produced very little gp120, vif, and nef proteins as compared to isolates from AIDS patients. Certain restriction endonuclease patterns, such as Bg1 II, were quite distinct for isolates from asymptomatic men as compared to AIDS patients. CONCLUSION: HIV isolates from asymptomatic men are biologically and genetically distinct from those of AIDS patients. Such differences may be related to the various clinical outcomes of HIV infection.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- CD4-Positive T-Lymphocytes
- DNA, Viral
- Gene Products, nef
- HIV
- HIV Antibodies
- HIV Core Protein p24
- HIV Envelope Protein gp120
- HIV Infections
- HIV Long Terminal Repeat
- HIV Seropositivity
- Humans
- In Vitro
- Male
- genetics
- immunology
Other ID:
UI: 102178685
From Meeting Abstracts