| Mass Spectra of Nematode Sterols |
Sterols are important biomolecules within nematodes because sterols function as membrane components and serve as metabolic precursors to steroid hormones. From the perspective of a nematode, sterols are nutritionally important because nematodes lack the ability to biosynthesize sterols, unlike plants or higher animals. Therefore, nematodes possess a nutritional requirement for sterols, which parasitic nematodes must obtain from their animal or plant hosts. This nutritional requirement can be exploited in the design of novel strategies for controlling agriculturally important plant-parasitic nematodes.
Consequently, a large part of David Chitwood’s research has focused on the biochemistry of sterols in nematodes. A large number of structurally diverse sterols have been isolated by us from nematodes or their hosts or their diets; these sterols have been identified via gas chromatography and mass spectrometry.
As a courtesy to the scientific community, we present a table containing mass spectral data of sterols isolated by us from nematodes or their hosts or diets. This table is based upon a similar table in Lipids, volume 26, pages 619-627, 1991, "Metabolism of plant sterols by nematodes", by D. J. Chitwood and W. R. Lusby. The U.S. Government has the right to retain a nonexclusive, royalty-free license in and to any copyright covering this paper.
Mass Spectral Data for Nematode Sterols, Analyzed as Steryl Acetate Derivativesa
|
| Steryl acetate |
Mass spectrum ((m/z, relative intensity to base peak) |
| Cholesta-5,7,9(11)-trienol |
424 (5), 364 (100), 349 (33), 251 (31), 209 (64), 197 (43), 195 (52) |
| Cholest-8(14)-enol |
428 (100), 413 (18), 368 (6), 353 (13), 315 (16), 288 (7), 273 (6), 255 (21), 229 (42), 213 (43), 81 (80), 55 (83) |
| Cholesterol |
368 (100), 353 (14), 260 (15), 255 (13), 247 (18), 213 (14), 147 (48), 145 (37), 81 (74), 55 (71) |
| Cholestanol |
430 (12), 415 (2), 370 (33), 355 (16), 316 (3), 276 (28), 275 (18), 257 (4), 230 (17), 215 (100), 201 (18), 147 (32), 81 (53) |
| Cholest-8(9)-enol |
428 (89), 413 (18), 368 (5), 353 (16), 315 (9), 255 (23), 229 (37), 213 (46), 55 (100). |
| Cholesta-7,9(11)-dienol |
426 (100), 411 (22), 366 (19), 351 (37), 313 (30), 286 (19), 271 (11), 259 (31), 253 (38), 211 (62), 199 (33), 55 (50) |
| Cholesta-5,7,9(11),24-tetraenol |
422 (4), 362 (63), 347 (15), 251 (18), 249 (14), 209 (47), 195 (39), 69 (100) |
| Desmosterol |
366 (51), 351 (11), 253 (23), 213 (10), 145 (24), 69 (100) |
| 7-Dehydrocholesterol |
426 (2), 366 (100), 351 (34), 253 (23), 211 (17), 199 (20), 158 (62), 143 (86), 55 (93) |
| 24-Methylcholesta-5,22(E)-dienol |
380 (100), 365 (5), 337 (3), 282 (7), 255 (49), 213 (11), 81 (78), 69 (82) |
| Cholest-24-enol |
428 (4), 413 (8), 353 (4), 344 (12), 317 (3), 315 (70), 257 (6), 255 (43), 229 (3), 215 (17), 69 (100) |
| Lathosterol |
428 (94), 413 (17), 368 (6), 353 (13), 315 (12), 288 (6), 273 (10), 255 (89), 229 (31), 213 (53), 81 (93), 55 (100) |
| 24-Methylcholest-22(E)-enol |
442 (18), 344 (29), 329 (3), 315 (36), 257 (57), 215 (14), 55 (100) |
| Cholesta-8(9),24-dienol |
426 (23), 411 (10), 351 (8), 315 (1), 313 (3), 255 (3), 229 (11), 213 (32), 69 (100), 55 (51) |
| Cholesta-5,7,9(11),22(E),24-pentaenol |
420 (1), 360 (14), 345 (1), 251 (14), 249 (7), 109 (100) |
| Cholesta-5,22(E),24-trienol |
424 (1), 364 (1), 313 (8), 282 (24), 255 (6), 253 (16), 213 (2), 199 (1), 109 (100) |
| Cholesta-5,7,24-trienol |
424 (3), 364 (98), 349 (20), 253 (16), 251 (10), 211 (15), 199 (13), 69 (100) |
| Cholesta-7,24-dienol |
426 (11), 411 (11), 351 (4), 315 (3), 313 (100), 255 (12), 229 (7), 213 (23), 69 (98), 55 (54) |
| 24-Methylcholesta-5,23-dienol |
380 (28), 365 (3), 296 (3), 283 (34), 259 (4), 255 (6), 253 (24), 227 (4), 215 (6), 213 (8), 159 (27), 145 (37), 133 (54), 55 (100) |
| 24-Methylcholesta-5,7,9(11)-trienol |
438(5), 378 (100), 363 (23), 251 (27), 225 (13), 209 (64), 195 (53), 158 (14), 143 (19), 55 (99) |
| 24-Methylenecholesterol |
380 (57), 365 (5), 296 (32), 281 (9), 253 (17), 213 (13), 81 (87), 55 (100) |
| Cholesta-5,7,22(E),24-tetraenol |
422 (1), 362 (26), 347 (3), 280 (1), 253 (10), 251 (6), 157 (27), 143 (22), 109 (100) |
| 24-Methylenecholestanol |
442 (1), 427 (1), 358 (46), 343 (11), 315 (21), 298 (2), 283 (4), 255 (11), 215 (23), 55 (100) |
| 24-Methylcholesterol |
382 (100), 367 (14), 274 (15), 261 (12), 255 (17), 213 (16), 147 (63), 81 (60) |
| 24-Methylcholestanol |
444 (17), 429 (2), 384 (31), 369 (16), 330 (2), 317 (1), 315 (1), 276 (33), 275 (21), 257 (6), 230 (14), 215 (100), 201 (19), 147 (34), 81 (53) |
| 24-Methylenecholesta-5,7-dienol |
438 (1), 378 (45), 363 (6), 253 (8), 227 (1), 211 (5), 158 (30), 143 (29), 55 (100) |
| 24-Ethylcholesta-5,22(E)-dienol |
394 (92), 379 (3), 351 (6), 313 (2), 282 (6), 255 (35), 228 (4), 213 (6), 81 (87), 55 (100) |
| 24-Methylcholesta-7,24(28)-dienol |
440 (3), 425 (3), 365 (2), 356 (22), 315 (2), 313 (100), 255 (14), 253 (9), 227 (12), 213 (26) |
| 24-Methylcholesta-5,7-dienol |
440 (1), 380 (100), 365 (14), 313 (1), 253 (37), 211 (18), 199 (19), 158 (81), 143 (79) |
| 24-Ethylcholest-22(E)-enol |
456 (9), 353 (9), 344 (14), 329 (2), 317 (2), 315 (19), 257 (36), 255 (8), 215 (5), 55 (100) |
| 24-Methylcholest-7-enol |
442 (40), 427 (6), 382 (3), 367 (5), 315 (7), 255 (100), 229 (29), 213 (52), 81 (67) |
| 24-Methylcholesta-5,24(25)-dienol |
380 (27), 365 (5), 296 (53), 281 (17), 253 (19), 213 (17), 83 (100) |
| 24-Ethylcholesta-5,22(E),24(28)-trienol |
452 (1), 392 (12), 313 (18), 282 (89), 255 (24), 253 (78), 213 (8), 157 (14), 143 (14), 137 (48), 95 (100) |
| 24-Ethylcholesta-5,23-dienol |
394 (100), 379 (7), 283 (26), 253 (22), 213 (11), 81 (78), 55 (96) |
| 24-Ethylcholesta-7,22(E)-dienol |
454 (21), 439 (4), 411 (7), 394 (4), 379 (4), 351 (6), 342 (13), 315 (13), 313 (86), 255 (37), 229 (14), 213 (15), 81 (93), 55 (100) |
| Fucosterol |
394 (23), 379 (2), 296 (100), 281 (14), 253 (8), 228 (6), 213 (13), 55 (92) |
| 24-Ethylcholesterol |
396 (100), 381 (14), 288 (13), 275 (13), 255 (19), 213 (17), 147 (52), 81 (56) |
| 24-Ethylcholestanol |
458 (18), 443 (2), 398 (32), 383 (13), 344 (3), 276 (23), 275 (21), 257 (6), 230 (15), 215 (100), 201 (17), 147 (34), 81 (49) |
| Isofucosterol |
394 (12), 379 (1), 296 (100), 281 (19), 253 (8), 228 (7), 213 (11), 55 (87) |
| Isofucostanol |
456 (2), 358 (83), 343 (15), 315 (7), 298 (9), 283 (10), 255 (8), 215 (24), 55 (100) |
| 24-Ethylcholesta-5,24(25)-dienol |
394 (32), 379 (6), 296 (100), 281 (23), 253 (18), 228 (8), 213 (9), 55 (81) |
| 24-Ethylcholesta-5,7-dienol |
454 (1), 394 (100), 379 (21), 253 (40), 211 (22), 199 (23), 158 (84), 143 (87) |
| 24-Ethyllathosterol |
456 (24), 441 (1), 396 (1), 381 (1), 315 (3), 255 (73), 229 (20), 213 (42), 81 (97), 55 (100) |
| 24-Ethylcholest-24(25)-enol |
456 (79), 441 (6), 358 (52), 343 (13), 315 (19), 283 (6), 255 (48), 229 (16), 213 (23), 55 (100) |
| 24Z-Ethylidenelathosterol |
454 (2), 439 (1), 394 (1), 356 (37), 313 (100), 296 (4), 281 (5), 255 (5), 253 (4), 213 (12), 55 (48) |
| 4a-Methylcholest-8(14)-enol |
442 (36), 427 (4), 382 (2), 367 (7), 329(4), 269 (14), 243 (23), 227 (34), 95 (73), 55 (100) |
| 4a-Methylcholestanol |
444 (17), 429 (4), 384 (48), 369 (30), 355 (7), 343 (4), 329 (1), 316 (1), 290 (26), 289 (17), 262 (13), 244 (21), 229 (100), 215 (23), 95 (74), 55 (59) |
| 4a-Methylcholesta-8(14),24-dienol |
440 (19), 425 (6), 365 (5), 329 (1), 327 (2), 269 (2), 243 (9), 227 (16), 69 (100), 55 (57) |
| 4a-Methylcholest-7-enol |
442 (33), 427 (4), 382 (4), 367 (6), 329(4), 269 (52), 243 (18), 227 (36), 95 (96), 55 (100) |
| 4a-Methylcholesta-7,24-dienol |
440 (1), 425 (3), 365 (1), 329 (1), 327 (67), 269 (4), 243 (2), 227 (10), 69 (100), 55 (68) |
| Obtusifoliol |
468 (28), 453 (64), 393 (22), 369 (9), 343 (4), 309 (11), 287 (19), 227 (14), 55 (100) |
| 4a,24-Dimethylcholest-8(14)-enol |
456 (20), 441 (2), 396 (1), 381 (2), 329 (3), 269 (9), 243 (15), 227 (23), 95 (64), 55 (100) |
| 4a,24-Dimethylcholestanol |
458 (18), 443 (3), 398 (48), 383 (31), 369 (7), 290 (32), 289 (18), 271 (6), 244 (23), 230 (57), 229 (100), 215 (22) |
| 4a,24-Dimethylcholest-7-enol |
456 (26), 441 (2), 396 (2), 381 (3), 329 (4), 269 (53), 243 (14), 227 (33), 95 (93), 55 (100) |
| 4a-Methyl-24-ethylcholest-22(E)-enol |
470 (28), 455 (2), 427 (2), 367 (17), 358 (24), 343 (4), 329 (23), 271 (33), 229 (8), 55 (100) |
| 4a-Methyl-24-methylenelathosterol |
454 (6), 439 (5), 394 (4), 379 (3), 370 (22), 327 (100), 269 (12), 241 (23), 227 (20) |
| 4a-Methyl-24-ethylcholestanol |
472 (16), 457 (3), 412 (47), 397 (24), 383 (7), 290 (32), 289 (10), 271 (7), 244 (21), 230 (56), 229 (100), 215 (23) |
| 4a-Methylisofucostanol |
470 (4), 455 (1), 372 (100), 357 (12), 329 (6), 312 (4), 269 (3), 230 (27), 229 (18) |
| 4a-Methyl-24(Z)-ethylidenelathosterol |
468 (12), 453 (9), 408 (6), 370 (33), 327 (100), 267 (5), 241 (9), 227 (7), 55 (65) |
| Cycloartanol |
470 (1), 455 (2), 410 (8), 395 (9), 367 (3), 357 (1), 355 (1), 341 (3), 297 (6), 288 (10), 273 (3), 55 (100) |
| Cycloeucalenol |
468 (4), 453 (7), 408 (62), 393 (37), 300 (6), 283 (8), 281 (7), 55 (100) |
| Cycloartenol |
468 (1), 453 (1), 408 (11), 393 (7), 365 (3), 339 (2), 297 (1), 295 (1), 286 (7), 271 (4), 257 (1), 255 (1), 243 (2), 69 (100) |
| 24-Methylenecycloartanol |
482 (1), 467 (1), 422 (9), 407 (6), 379 (4), 353 (1), 300 (4), 297 (2), 285 (1), 55 (100) |
a Numbers in parentheses are relative abundance (%).
Electron impact spectra were obtained in a Finnigan 4510 mass spectrometer equipped with a 15-meter x 0.32-mm DB-1 capillary column and connected to an Incos data system. Mention of trade names or commercial products in this table is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.
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