From RPRDANI@TECHNION.BITNET Sun Apr 14 16:20:17 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA18720; Wed, 14 Apr 1993 02:55:21 -0500 Return-Path: Received: from TECHNION.TECHNION.AC.IL (MAILER@TECHNION) by vx.cis.umn.edu (PMDF #2574 ) id <01GWZI5EGUF49AN5E4@vx.cis.umn.edu>; Wed, 14 Apr 1993 02:54:40 CDT Received: from TECHNION (RPRDANI) by TECHNION.TECHNION.AC.IL (Mailer R2.07) with BSMTP id 2007; Wed, 14 Apr 93 10:51:18 IST In-Reply-To: Message of 13 Apr 1993 15:54:09 -0500 from Message-Id: <01GWZI5EGUF69AN5E4@vx.cis.umn.edu> X-Envelope-To: nih-image@soils.umn.edu From: dd To: nih-image@soils.umn.edu Subject: Re: On-line PLOT Date: 14 Apr 1993 10:50:17 +0530 (IST) Content-Transfer-Encoding: 7BIT Thanks, Wayne I'll try to Macro the On-line "Brightness vs time" plot. danni From wayne@zippy.nimh.nih.gov Wed Apr 14 10:56:26 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA20426; Wed, 14 Apr 1993 10:58:36 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03405; Wed, 14 Apr 93 12:07:05 -0400 Message-Id: <9304141607.AA03405@zippy.nimh.nih.gov> Date: Wed, 14 Apr 1993 10:56:26 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Image bombs until I delete >THe INIT has since been identified as the new INIT 17 Mac viruys. Can be >eliminated by Disinfectant 3.1 I have uploaded Disinfectant 3.1 to the util directory on zippy.nimh.nih.gov. --wayne From wayne@zippy.nimh.nih.gov Wed Apr 14 12:58:36 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA21082; Wed, 14 Apr 1993 13:00:44 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03728; Wed, 14 Apr 93 14:09:14 -0400 Message-Id: <9304141809.AA03728@zippy.nimh.nih.gov> Date: Wed, 14 Apr 1993 12:58:36 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Adding to a segmented line >I seem to be having problems adding to a segmented line as indicated in the manu >al. I select a broken line by multiple clicks and end the selection with a doubl >e click. I then press the control key while making a second selection in the >hope that the length of both selections will be added in one result but the >original selection disappears. Am I doing something wrong or I just >missinterpreted the manual??? You cannot use the control key with the line tool to extend an existing line selection. You can, however, add rectangular, oval or freehand selections to a line selections. This restriction exists because Image uses QuickDraw regions to support editing of selections. --wayne From geller@synapse.umdnj.edu Wed Apr 14 10:14:58 1993 Received: from synapse.UMDNJ.EDU by nx1.soils.umn.edu (5.65c) id AA21315; Wed, 14 Apr 1993 13:19:45 -0500 Return-Path: Date: Wed, 14 Apr 93 14:14:58 EDT From: "Herbert M. Geller" To: nih-image@soils.umn.edu Subject: Ratio Imaging Message-Id: <9304141414.aa23625@synapse.umdnj.edu> To image users: I have a modest amount of end-of-year grant money to spend on imaging equipment. Right now, I have both Mac & PCs in the lab and can use either. If I could have my 'druthers, I would like to have the ability to do 1) time lapse image capture, including the use of autofocus attachments & stage controllers (Ludl or other); and 2) calcium ratio imaging. Now I realize that the ideal ratio imaging is done with a cooled CCD camera, but I believe that is out of my price range. Already on hand is a microchannel image intensifier. In my preliminary thoughts, I have put together the following as likely purchases: 1) DAGE CCD-72 camera & controller 2) Ludl 2000 box, filter wheels, autofocus and stage controller 3) Scion frame grabber board. 4) Optical disk recorder. Now, if there are any of you out there that have experience with my proposed applications & this equipment, I would appreciate your advice. I am of the suspicion that in order to do fura-2 imaging with this hardware, I will need to average; therefore, will this equipment do it? Maybe all I need is extra memory for the frame grabber, or do I need a different frame grabber? Will the ludl box work fast enough? Herb Geller (Maybe all i From wayne@zippy.nimh.nih.gov Wed Apr 14 13:22:15 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA21412; Wed, 14 Apr 1993 13:27:06 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03774; Wed, 14 Apr 93 14:32:40 -0400 Message-Id: <9304141832.AA03774@zippy.nimh.nih.gov> Date: Wed, 14 Apr 1993 13:22:15 +0000 To: WILLI JAHNEN-DECHENT From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: frame grabber cards for IMAGE Cc: nih-image@soils.umn.edu >can you recommend any frame grabber card for Mac LCII and LC III computers >to use with your IMAGE software? In the documentation on IMAGE I found only >cards for NUBUS Macs. Thank you, Image 1.50 will support any frame grabber or scanner that has a Photoshop plug-in. The only frame grabber I know of for the LC is the SuperMac Video Spigot, but I don't know if a plug-in is available for it. I am currently testing V1.50's plug-in support using a LaCie SilverScanner flatbed scanner. --wayne From wayne@zippy.nimh.nih.gov Wed Apr 14 13:35:20 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA21516; Wed, 14 Apr 1993 13:37:30 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03794; Wed, 14 Apr 93 14:45:58 -0400 Message-Id: <9304141845.AA03794@zippy.nimh.nih.gov> Date: Wed, 14 Apr 1993 13:35:20 +0000 To: anupm@pop.nih.gov From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Drawing polygon ROI through macros / Pascal Cc: nih-image@soils.umn.edu > I am working on an application where I need to compute the area of >polygon cells for which I have the coordinates stored in a file. Is there a >way I can use these coordinates to draw a polygon ROI using macro or pascal >routine and then use the standard area measurement technique to get the >area etc.. One way you might be able to this would be to use the "Plot XYZ macro"(in "More Macros") to plot the polygon, then use the wand tool to outline it and Measure to compute the area. --wayne From nih-image@soils.umn.edu Wed Apr 14 13:00:57 1993 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA21105; Wed, 14 Apr 1993 13:00:57 -0500 Return-Path: Date: Wed, 14 Apr 1993 13:00:57 -0500 Message-Id: <9304141809.AA03728@zippy.nimh.nih.gov> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: wayne@zippy.nimh.nih.gov(Wayne Rasband) To: Multiple recipients of list Subject: Re: Adding to a segmented line >I seem to be having problems adding to a segmented line as indicated in the manu >al. I select a broken line by multiple clicks and end the selection with a doubl >e click. I then press the control key while making a second selection in the >hope that the length of both selections will be added in one result but the >original selection disappears. Am I doing something wrong or I just >missinterpreted the manual??? You cannot use the control key with the line tool to extend an existing line selection. You can, however, add rectangular, oval or freehand selections to a line selections. This restriction exists because Image uses QuickDraw regions to support editing of selections. --wayne From wayne@zippy.nimh.nih.gov Thu Apr 15 16:32:36 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA26742; Thu, 15 Apr 1993 16:34:48 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA05509; Thu, 15 Apr 93 17:43:17 -0400 Message-Id: <9304152143.AA05509@zippy.nimh.nih.gov> Date: Thu, 15 Apr 1993 16:32:36 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Ratio Imaging > 1) DAGE CCD-72 camera & controller > 2) Ludl 2000 box, filter wheels, autofocus and stage controller > 3) Scion frame grabber board. > 4) Optical disk recorder. > > Now, if there are any of you out there that have experience with >my proposed applications & this equipment, I would appreciate your advice. > > I am of the suspicion that in order to do fura-2 imaging with this >hardware, I will need to average; therefore, will this equipment do it? >Maybe all I need is extra memory for the frame grabber, or do I need >a different frame grabber? Will the ludl box work fast enough? Adding more memory to the LG-3 allows you to capture frames at video rates but the averaging stii has to be done in software. A 16MB LG-3 can capture 32 frames in about one second, but it takes Image 10 or 15 seconds to compute the average. One way to do real-time averaing using the LG-3 is to get Dage's $4000 DSP-200 digital image processor. --wayne From wayne@zippy.nimh.nih.gov Thu Apr 15 16:58:53 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA26951; Thu, 15 Apr 1993 17:01:05 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA05550; Thu, 15 Apr 93 18:09:34 -0400 Message-Id: <9304152209.AA05550@zippy.nimh.nih.gov> Date: Thu, 15 Apr 1993 16:58:53 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: WaitForTrigger bug in 1.49b5? >I have been using WatiForTrigger in macros that need to act upon a mouse >click. >Thle Button macro command hasn't worked in quite the way I needed. While >the macros work fine with 1.49b5 on a system with a frame grabber card, >most of my development is on a Mac without a capture card (our Image >system is in continuous use and if I'm gooing to work at night, i'd rather >do it at home). > On a machine without a framegrabber card invoking a macro with the >WaitForTrigger command results in the following error message: "External >triggering requires a Quick Capture Card". The macros were written with >1.47, and as I recall, worked with 1.46b2, on my IIci without a capture card. Couldn't you use the following procedure instead of WaitForTrigger? procedure WaitForButton; begin repeat until button; repeat until not button; end; The second repeat loop prevents "auto-repeating" if the mouse button is held down too long. Without it, WaitForButton and WaitForTrigger should behave the same way. --wayne From glenmac@u.washington.edu Thu Apr 15 08:28:11 1993 Received: from carson.u.washington.edu by nx1.soils.umn.edu (5.65c) id AA27176; Thu, 15 Apr 1993 17:43:53 -0500 Return-Path: Received: by carson.u.washington.edu (5.65/UW-NDC Revision: 2.22 ) id AA28629; Thu, 15 Apr 93 15:43:15 -0700 Sender: glenmac@carson.u.washington.edu Date: Thu, 15 Apr 1993 15:28:11 -0700 (PDT) From: Glen Macdonald Subject: Re: WaitForTrigger bug in 1.49b5? To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <9304152209.AA05550@zippy.nimh.nih.gov> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=us-ascii Yes, replacing WaitForTrigger with "repeat until button" solved the problem. thanks to Ed Huff for a prior reply about that. I had stayed away from the repeat until button because my earlier variants on that resulted in loops of repeated button counts. repeat until x<0 or y<0 to terminate the counting macros ran afoul of the new NotInBounds error trap in utilities.p (I'm getting more adventursome messing around in the source), so I then added KillRoi; at the bottom of the macro terminating statements to get around it. the macro will be posted to /contrib on zippy as Cavalieri soon as I get the doc. written. thanks for the help. -Glen From JONKER@amc.uva.nl Tue Apr 16 09:20:39 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA29042; Fri, 16 Apr 1993 02:05:56 -0500 Return-Path: Received: from HASARA5.BITNET (MAILGATE@HASARA5) by vx.cis.umn.edu (PMDF #2574 ) id <01GX28ZQC9XC9ANPYJ@vx.cis.umn.edu>; Fri, 16 Apr 1993 02:05:10 CDT Received: from SARA.NL by SARA.NL for nih-image@soils.umn.edu; 16 Apr 93 9:04 MET Received: from amc.uva.nl by SARA.NL with PMDF#10201; Fri, 16 Apr 1993 08:20 MET Received: from amc.uva.nl by amc.uva.nl (PMDF #2498 ) id <01GX2LYRGAKW0002UK@amc.uva.nl>; Fri, 16 Apr 1993 08:20:39 MET Date: 16 Apr 1993 08:20:39 +0100 (MET) From: JONKER@amc.uva.nl Subject: subscribe address, in the docs? moderated list? To: nih-image@soils.umn.edu Message-Id: <01GX2LYRGAKY0002UK@amc.uva.nl> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Transfer-Encoding: 7BIT Is the address of the list, and how to subscribe, in the docs from image1.49 on? Is this the proper address to do the subscription (asking for a fellow user) Is the list moderated, or should I presume that my message has gone oblivious if I don't find it in the mailing after a few days (which has happened lately) If the list is moderated, I don't expect this message to appear on the list, but I would like to receive an acknowledge of receipt, to prevent a catch-22. Ard From jladwig@soils.umn.edu Fri Apr 16 06:42:52 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA01162; Fri, 16 Apr 1993 11:44:29 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA05649; Fri, 16 Apr 93 11:42:52 CDT Date: Fri, 16 Apr 93 11:42:52 CDT From: "John Ladwig" Message-Id: <9304161642.AA05649@saturn.soils.umn.edu> To: Subject: Re: subscribe address, in the docs? moderated list? In-Reply-To: JONKER@amc.uva.nl's message <01GX2LYRGAKY0002UK@amc.uva.nl> of 16 April 1993 References: <01GX2LYRGAKY0002UK@amc.uva.nl> JONKER@amc.uva.nl writes on 16 April 1993 at 02:14:12 -0500 > Is this the proper address to do the subscription (asking for a > fellow user) My standard "so you're trying to subscribe" posting: ======================================================================== If you are trying to subscribe to the list, send the following as the *first* line of a message to "listserv@soils.umn.edu" sub nih-image Your Name Where Your Name is your real (English) given name. Your email address will be deciphered by the listserv software. -jml NIH-Image list administrator. ======================================================================== > If the list is moderated, I don't expect this message to appear > on the list, but I would like to receive an acknowledge of receipt, > to prevent a catch-22. You can control whether you see copies of your own postings via the set nih-image mail ack or set nih-image mail noack control messages, sent to "listserv@soils.umn.edu". > Is the list moderated, or should I presume that my message has > gone oblivious if I don't find it in the mailing after a > few days (which has happened lately) This list is not moderated. If you have your subscription set to see your own posts (which is the default subscription configuration), and you don't see the message back in a fairly short time, you may assume that it didn't make the list. It is possible for the listserver software to be down for a short while, or the machine which hosts it, but both have been *very* reliable. This is the first message from you I've seen in over a week. These are all the messages from the list in the last week or so; you should have seen all but one: ======================================================================== Apr 10 19:21:03 nx1 sendmail[6691]: AA06691: message-id= Apr 12 12:28:40 nx1 sendmail[11713]: AA11713: message-id=<9304121725.AA23528@mailgate.roche.com> Apr 12 13:12:23 nx1 sendmail[11925]: AA11925: message-id=<9304121543.AA27844@ecuanex.ec> Apr 12 14:08:24 nx1 sendmail[12186]: AA12186: message-id= Apr 13 04:23:25 nx1 sendmail[14498]: AA14498: message-id=<9304130917.AA10902@decnet.sunet.se> Apr 13 07:23:54 nx1 sendmail[15129]: AA15129: message-id=<9304131221.AA03437@helix.nih.gov> Apr 13 15:53:49 nx1 sendmail[17024]: AA17024: message-id=<9304132100.AA02321@zippy.nimh.nih.gov> Apr 13 15:55:59 nx1 sendmail[17069]: AA17069: message-id=<199304132053.AA17020@nx1.soils.umn.edu> Apr 14 02:56:52 nx1 sendmail[18754]: AA18754: message-id=<01GWZI5EGUF69AN5E4@vx.cis.umn.edu> Apr 14 11:00:55 nx1 sendmail[20462]: AA20462: message-id=<9304141607.AA03405@zippy.nimh.nih.gov> Apr 14 13:01:58 nx1 sendmail[21115]: AA21115: message-id=<9304141809.AA03728@zippy.nimh.nih.gov> Apr 14 13:21:27 nx1 sendmail[21368]: AA21368: message-id= <9304141414.aa23625@synapse.umdnj.edu> Apr 14 13:28:36 nx1 sendmail[21446]: AA21446: message-id=<9304141832.AA03774@zippy.nimh.nih.gov> Apr 14 13:38:55 nx1 sendmail[21551]: AA21551: from=, size=948, class=0, received from localhost (127.0.0.1) Apr 15 16:36:27 nx1 sendmail[26800]: AA26800: message-id=<9304152143.AA05509@zippy.nimh.nih.gov> Apr 15 17:02:53 nx1 sendmail[26989]: AA26989: message-id=<9304152209.AA05550@zippy.nimh.nih.gov> Apr 15 17:45:28 nx1 sendmail[27222]: AA27222: message-id= Apr 16 02:17:04 nx1 sendmail[29098]: AA29098: message-id=<01GX2LYRGAKY0002UK@amc.uva.nl> Apr 10 19:21:03 nx1 sendmail[6691]: AA06691: message-id= Apr 12 12:28:40 nx1 sendmail[11713]: AA11713: message-id=<9304121725.AA23528@mailgate.roche.com> Apr 12 13:12:23 nx1 sendmail[11925]: AA11925: message-id=<9304121543.AA27844@ecuanex.ec> Apr 12 14:08:24 nx1 sendmail[12186]: AA12186: message-id= Apr 13 04:23:25 nx1 sendmail[14498]: AA14498: message-id=<9304130917.AA10902@decnet.sunet.se> Apr 13 07:23:54 nx1 sendmail[15129]: AA15129: message-id=<9304131221.AA03437@helix.nih.gov> Apr 13 15:53:49 nx1 sendmail[17024]: AA17024: message-id=<9304132100.AA02321@zippy.nimh.nih.gov> Apr 13 15:55:59 nx1 sendmail[17069]: AA17069: message-id=<199304132053.AA17020@nx1.soils.umn.edu> Apr 14 02:56:52 nx1 sendmail[18754]: AA18754: message-id=<01GWZI5EGUF69AN5E4@vx.cis.umn.edu> Apr 14 11:00:55 nx1 sendmail[20462]: AA20462: message-id=<9304141607.AA03405@zippy.nimh.nih.gov> Apr 14 13:01:58 nx1 sendmail[21115]: AA21115: message-id=<9304141809.AA03728@zippy.nimh.nih.gov> Apr 14 13:21:27 nx1 sendmail[21368]: AA21368: message-id= <9304141414.aa23625@synapse.umdnj.edu> Apr 14 13:28:36 nx1 sendmail[21446]: AA21446: message-id=<9304141832.AA03774@zippy.nimh.nih.gov> Apr 14 13:38:55 nx1 sendmail[21551]: AA21551: from=, size=948, class=0, received from localhost (127.0.0.1) Apr 15 16:36:27 nx1 sendmail[26800]: AA26800: message-id=<9304152143.AA05509@zippy.nimh.nih.gov> Apr 15 17:02:53 nx1 sendmail[26989]: AA26989: message-id=<9304152209.AA05550@zippy.nimh.nih.gov> Apr 15 17:45:28 nx1 sendmail[27222]: AA27222: message-id= Apr 16 01:56:30 nx1 sendmail[29000]: AA29000: to="|IFS=' ';exec /usr/local/src/procmail-2.81/new/procmail -t #jladwig", delay=00:00:08, stat=Sent, mailer=prog Apr 16 02:17:04 nx1 sendmail[29098]: AA29098: message-id=<01GX2LYRGAKY0002UK@amc.uva.nl> ======================================================================== This is the only bounce I've gotten for you within the last week: ======================================================================== From: PMDF Mail Server To: postmaster@HEARNVAX.nic.SURFnet.nl, owner-nih-image@soils.umn.edu Subject: Undeliverable mail: SMTP delivery failure Date: 14 Apr 1993 10:15:02 +0100 (MET) Content-Type: MULTIPART/MIXED; BOUNDARY="Boundary (ID UHftzv1n3M2EQyoKdv9Ceg)" Content-Transfer-Encoding: 7BIT --Boundary (ID UHftzv1n3M2EQyoKdv9Ceg) The message could not be delivered to: Addressee: jonker@amc.uva.nl Reason: Illegal host/domain name found. --Boundary (ID UHftzv1n3M2EQyoKdv9Ceg) Received: from mail.tc.umn.edu by HEARNVAX.nic.SURFnet.nl (PMDF #3330 ) id <01GWZWX4OAA8005TV9@HEARNVAX.nic.SURFnet.nl>; Wed, 14 Apr 1993 09:57:56 MET Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012bcbc3a5016424; Wed, 14 Apr 93 02:56:22 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA18754; Wed, 14 Apr 1993 02:56:46 -0500 Date: 14 Apr 1993 02:56:46 -0500 From: dd Subject: Re: On-line PLOT Sender: nih-image@soils.umn.edu To: Multiple recipients of list Errors-to: owner-nih-image@soils.umn.edu Reply-to: nih-image@soils.umn.edu Message-id: <01GWZI5EGUF69AN5E4@vx.cis.umn.edu> X-Envelope-to: jonker@amc.uva.nl Content-transfer-encoding: 7BIT Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas Thanks, Wayne I'll try to Macro the On-line "Brightness vs time" plot. danni --Boundary (ID UHftzv1n3M2EQyoKdv9Ceg)-- ======================================================================== From jladwig@soils.umn.edu Fri Apr 16 06:45:18 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA01208; Fri, 16 Apr 1993 11:46:54 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA05669; Fri, 16 Apr 93 11:45:18 CDT Date: Fri, 16 Apr 93 11:45:18 CDT From: "John Ladwig" Message-Id: <9304161645.AA05669@saturn.soils.umn.edu> To: Subject: Re: subscribe address, in the docs? moderated list? In-Reply-To: JONKER@amc.uva.nl's message <01GX2LYRGAKY0002UK@amc.uva.nl> of 16 April 1993 References: <01GX2LYRGAKY0002UK@amc.uva.nl> JONKER@amc.uva.nl writes on 16 April 1993 at 02:14:12 -0500 > Is this the proper address to do the subscription (asking for a > fellow user) My standard "so you're trying to subscribe" posting: ======================================================================== If you are trying to subscribe to the list, send the following as the *first* line of a message to "listserv@soils.umn.edu" sub nih-image Your Name Where Your Name is your real (English) given name. Your email address will be deciphered by the listserv software. -jml NIH-Image list administrator. ======================================================================== > If the list is moderated, I don't expect this message to appear > on the list, but I would like to receive an acknowledge of receipt, > to prevent a catch-22. You can control whether you see copies of your own postings via the set nih-image mail ack or set nih-image mail noack control messages, sent to "listserv@soils.umn.edu". > Is the list moderated, or should I presume that my message has > gone oblivious if I don't find it in the mailing after a > few days (which has happened lately) This list is not moderated. If you have your subscription set to see your own posts (which is the default subscription configuration), and you don't see the message back in a fairly short time, you may assume that it didn't make the list. It is possible for the listserver software to be down for a short while, or the machine which hosts it, but both have been *very* reliable. From mvivino@helix.nih.gov Fri Apr 16 09:24:10 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA01557; Fri, 16 Apr 1993 12:24:48 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA11657; Fri, 16 Apr 93 13:24:10 -0400 Date: Fri, 16 Apr 93 13:24:10 -0400 Message-Id: <9304161724.AA11657@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Personal engineering mag About a month ago, an editor of personal engineering magazine requested a paper on Image through this net. I sent him email asking more details, but he never replied. Did anyone ever get in touch with the guy? Mark From catherine_tsao@maca.sarnoff.com Fri Apr 16 12:33:32 1993 Received: from nova (sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA01653; Fri, 16 Apr 1993 12:33:32 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA28544; Fri, 16 Apr 93 13:32:52 EDT Message-Id: <9304161732.AA28544@nova> Date: 16 Apr 1993 13:23:10 U From: "Catherine Tsao" Subject: unsibscribe NIH-IMAGE To: "nih image" unsibscribe NIH-IMAGE unsibscribe NIH-IMAG From enater@soils.umn.edu Fri Apr 16 09:34:22 1993 Received: from forsoil.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA02227; Fri, 16 Apr 1993 14:29:21 -0500 Return-Path: Received: by forsoil.soils.umn.edu (4.1) id AA13777; Fri, 16 Apr 93 14:34:22 CDT From: "Ed Nater" Message-Id: <9304161934.AA13777@forsoil.soils.umn.edu> Subject: Re: unsibscribe NIH-IMAGE To: nih-image@soils.umn.edu Date: Fri, 16 Apr 1993 14:34:22 -0500 (CDT) In-Reply-To: <9304161732.AA28544@nova> from "Catherine Tsao" at Apr 16, 93 12:33:45 pm X-Mailer: ELM [version 2.4 PL20] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 192 Catherine - > > unsibscribe NIH-IMAGE > unsibscribe NIH-IMAG > > > Unsuscribe messages should be sent to: LISTSERV@soils.umn.edu. (they also need to be spelled correctly :-). Ed From wayne@zippy.nimh.nih.gov Fri Apr 16 15:49:25 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA02712; Fri, 16 Apr 1993 15:51:37 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06836; Fri, 16 Apr 93 17:00:06 -0400 Message-Id: <9304162100.AA06836@zippy.nimh.nih.gov> Date: Fri, 16 Apr 1993 15:49:25 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Ratio Imaging System ImproVision has a ratio imaging package that does ratio imaging with full support for real-time averaging, filter wheel control, etc. The package is called "ionVision" and is developed and distributed by: ImproVision Barclays Venture Centre, University of Warwick Science Park Sir William Lyons Road, Coventry, UK CV4 7EZ (011)44-203-692229 Quoting from their promotional material: "ionVision is an intracellular ion analysis system suitable for use with fluorescence dyes such as Fura-2, BCECF, Indo-1, etc. The system software allows control of a variety of external devices, eg, fast filter changers, electronic shutters, strobes, microscope stages, focus controls and integrating cameras. ionVision allows the acquisition of image sequences at video rates and will store the images to SmartStore, RAM, or Hard Disc. ionVision will enhance, process, and ratio the image sequences to produce a calibrated movie which can be animated or quantitated to produce charts of ion concentration changes as a function of time." This package uses Perceptics PixelTools boards to achieve the real-time video processing rates. Perceptics (Roger Leach) can supply limited information about the package if ImproVision cannot be reached. Their phone number is (615)966-9200. --wayne From kartenh@Sdsc.Edu Fri Apr 16 21:54:05 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA03037; Fri, 16 Apr 1993 16:55:16 -0500 Return-Path: Date: Fri, 16 Apr 93 21:54:05 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930416215405.22c1817c@sds.sdsc.edu> Subject: RE: Ratio Imaging System To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Does the ImproVision system work on the Mac? HJKarten From WETZEL@metro.bst.rochester.edu Fri Apr 16 14:25:00 1993 Received: from metro.bst.rochester.edu by nx1.soils.umn.edu (5.65c) id AA03255; Fri, 16 Apr 1993 17:25:35 -0500 Return-Path: Received: from METRO by METRO (PMDF #12506) id <01GX378FRIO099DGZN@METRO>; Fri, 16 Apr 1993 18:25 EDT Date: Fri, 16 Apr 1993 18:25 EDT From: WETZEL@metro.bst.rochester.edu Subject: NIH Image and X-Y stage To: nih-image@soils.umn.edu Message-Id: <01GX378FRIO099DGZN@METRO> X-Vms-To: IN%"nih-image@soils.umn.edu" Does anybody have a macro that permits NIH Image to work with an X-Y stage driver such as an LEP? DM Wetzel From nas16@phy.cam.ac.uk Sat Apr 17 00:07:35 1993 Received: from wcse0.phy.cam.ac.uk by nx1.soils.umn.edu (5.65c) id AA03477; Fri, 16 Apr 1993 18:07:22 -0500 Return-Path: Received: from [131.111.72.41] by wcse0.phy.cam.ac.uk with smtp (Smail3.1.28.1 #7) id m0njzUY-000E5zC; Sat, 17 Apr 93 00:06 WET DST Message-Id: Date: Sat, 17 Apr 1993 00:07:35 +0000 To: nih-image@soils.umn.edu From: nas16@phy.cam.ac.uk (Nick Safford) X-Sender: nas16@wcse0.phy.cam.ac.uk Subject: Re: Greyscale erosion >Image does erosion and dilation under the binary menu. Ah, yes, but only on binary images; _greyscale_ erosion is subtly different, (at least as explained to me in the Visilog manual) and is useful for detecting local maxima of intensity, regardless of their absolute brightness. It's very good for distinguishing haloes round cells. It may be that if I was cleverer I could do the same trick by working with lots of binary images, each one generated from the original greyscale image with a different threshold. Speed is pretty important to me (I have to do a lot of these operations on a lot of images) so ideally I'm looking for some nice fast pascal. (It's an indication of my enthusiasm for Image that I still want to use it despite having $6000 worth of Visilog - which is what it costs over here - even though Visilog does, in principle, do all the things I need!) Nick Safford 0223 337319 Cavendish Lab Madingley Rd Cambridge UK CB3 0HE From STELZER@EMBL-Heidelberg.DE Sat Apr 17 11:08:53 1993 Received: from eros.EMBL-Heidelberg.DE by nx1.soils.umn.edu (5.65c) id AA04852; Sat, 17 Apr 1993 03:09:37 -0500 Return-Path: Received: from EMBL-Heidelberg.DE by EMBL-Heidelberg.DE (PMDF V4.2-10 #2491) id <01GX445XPGE88Y5QBR@EMBL-Heidelberg.DE>; Sat, 17 Apr 1993 10:08:53 +0100 Date: Sat, 17 Apr 1993 10:08:53 +0100 From: STELZER@EMBL-Heidelberg.DE Subject: Hamamatsu Frame Grabbers To: nih-image@soils.umn.edu Message-Id: <01GX445XPZOI8Y5QBR@EMBL-Heidelberg.DE> X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Dear NIH Imagers!! Hamamatsu is offering frame grabber boards for the Macintosh line. Is anybody out there with some experience and are they supported by NIH image?? Thank You Ernst Stelzer Stelzer@EMBL-Heidelberg.DE From kartenh@Sdsc.Edu Sat Apr 17 15:50:47 1993 Received: from Sdsc.Edu (m5.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA00587; Sat, 17 Apr 1993 10:49:45 -0500 Return-Path: Date: Sat, 17 Apr 93 15:50:47 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930417155047.20402db6@m5.sdsc.edu> Subject: RE: Hamamatsu Frame Grabbers To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Do you have an further details on the Hamamatsu Frame Grabbers for the Mac? 16 bit? 1,000 line? Frames/sec? HJ Karten From mvivino@helix.nih.gov Mon Apr 19 04:43:45 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA05941; Mon, 19 Apr 1993 07:42:31 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA26173; Mon, 19 Apr 93 08:43:45 -0400 Date: Mon, 19 Apr 93 08:43:45 -0400 Message-Id: <9304191243.AA26173@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: Greyscale erosion > _greyscale_ erosion is subtly >different, (at least as explained to me in the Visilog manual) and is >useful for detecting local maxima of intensity, regardless of their >absolute brightness. It's very good for distinguishing haloes round cells. Have you tried laplacian and gradient convolutions, or possibly others? say like this or in a bigger matrix (4x4 or larger): -1 -1 -1 -1 +8 -1 -1 -1 -1 You could also make gradient operations along lines of the image detect local maxima. Perform something like -1,0,0,4,0,0,-1, or just fine tune it to whatever works. The beauty of image processing is that all the stuff in books may only get you started, then you just heuristically pick something and make it work better. Maybe anyway. Mark From wayne@zippy.nimh.nih.gov Mon Apr 19 08:40:53 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA06199; Mon, 19 Apr 1993 08:41:16 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA08300; Mon, 19 Apr 93 09:51:41 -0400 Message-Id: <9304191351.AA08300@zippy.nimh.nih.gov> Date: Mon, 19 Apr 1993 08:40:53 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: RE: Ratio Imaging System and frame grabber plug-ins > Does the ImproVision system work on the Mac? It is my understanding that it is a Mac based system. I get the information about it from Paul Whalen of Perceptics, who was afraid to post it for fear of being accused of commercialism. Paul also sent me a Photoshop plug-in he wrote for their PixelPipe frame grabber card and it seems to work fine with the Image 1.50 beta. This is the first frame grabber known to work with Image using the new plug-in interface. Mark Vivino tried RasterOps' plug-in for their frame grabber cards, but has not been able to get it to work. The problem seems to be that the RasterOps plug-in insists on running in 24-bit mode and Image likes to run in 8-bit mode. The obvious work-around, which Mark hasn't tried yet, is to use two monitors, one for the RasterOps card running in 24-bit mode, and one for Image running in 8-bit mode. --wayne From @cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be Tue Apr 20 06:41:32 1993 Received: from cc1.kuleuven.ac.be by nx1.soils.umn.edu (5.65c) id AA10358; Tue, 20 Apr 1993 06:41:32 -0500 Return-Path: <@cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be> Received: from rc1.vub.ac.be by cc1.kuleuven.ac.be (IBM VM SMTP V2R2) with TCP; Tue, 20 Apr 93 13:42:02 +0200 Received: from dec5.ulb.ac.be.ulb.ac.be (dbmdec5) by rc1.vub.ac.be (4.1/RC1-930416) id AA09106; Tue, 20 Apr 93 13:42:56 +0200 Received: by dec5.ulb.ac.be.ulb.ac.be (5.65/ULB.920908) id AA18626; Tue, 20 Apr 1993 13:40:44 +0100 From: jvanheld@dbm.ulb.ac.be (Jacques VAN HELDEN) Message-Id: <9304201240.AA18626@dec5.ulb.ac.be.ulb.ac.be> Subject: Re: NIH Image and X-Y stage To: nih-image@soils.umn.edu Date: Tue, 20 Apr 93 13:40:44 WET DST In-Reply-To: <01GX378FRIO099DGZN@METRO>; from "WETZEL%metro.bst.rochester.edu@mail.tc.umn.edu" at Apr 16, 93 5:29 pm X-Mailer: ELM [version 2.3 PL11] >Does anybody have a macro that permits NIH Image to work with >an X-Y stage driver such as an LEP? DM Wetzel There is an English society, Improvision, which adapted NIH-image for the control of peripheral such as filter wheel, XYZ stage driver, ... Their address is ImproVision Image Processing& Video Co Ltd Barclay Venture center University of Warwick Science Park St Williams Lyons Road Coventry CV4 7EZ Tel: (44) 203 692229 Fax: (44) 203 690091 By the way, I am personnaly looking for XYZ stage drivers. Could you indicate me the address(es) of suppliers that you found ? Thank you. * Jacques van Helden * Universite Libre de Bruxelles * Service de Neurobiologie * tel: (32) 2 650 99 41 * fax: (32) 2 650 97 44 * e-mail: jvanheld@ulb.ac.be From set@eru.mt.luth.se Tue Apr 20 18:16:29 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA10807; Tue, 20 Apr 1993 09:15:22 -0500 Return-Path: Received: from [130.240.1.174] (osse217.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA01252; Tue, 20 Apr 1993 16:16:29 +0200 Date: Tue, 20 Apr 1993 16:16:29 +0200 Message-Id: <199304201416.AA01252@eru.mt.luth.se> To: nih-image@soils.umn.edu From: set@mt.luth.se (Sven-Erik Tiberg) Subject: PAL/NTSC version of Image 1.49 for b-test. X-Content-Type: Text/Quoted-Readable In our application for Image we are looking at particles in flow, and tryes to track a specific particle trough images in a stack. As we could have high flow rates we tried to decreesed "delay between frames" and saw that this delay was always a multipel of 1/60 sec. And as we in europe are working with PAL system 25 Frames a second this coused an non equidistant time delay at 5 or more frames a second. With help from Wane and Apple we could solve this problem. The new version of Image1.49.PAL/NTSC are awalible at contrib/image/pub @ zippy.nimh.nih.gov if anyone are interested, and if you found any bug please report to me. I did by misstake send it as Image1.50b1, if system.manger at zippy would kind to rename it to for example Image1.49.PAL/NTSC I would be wery greatfull since it's a not any official version. And this could couse some problem with rev. repports. ----- Sven Erik Tiberg Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From salzman@Athena.MIT.EDU Wed Apr 21 07:58:01 1993 Received: from Athena.MIT.EDU (ATHENA-AS-WELL.MIT.EDU) by nx1.soils.umn.edu (5.65c) id AA10661; Wed, 21 Apr 1993 10:56:42 -0500 Return-Path: Received: from CASCADE.MIT.EDU by Athena.MIT.EDU with SMTP id AA00678; Wed, 21 Apr 93 11:58:04 EDT From: salzman@Athena.MIT.EDU Received: by cascade (5.57/4.7) id AA07266; Wed, 21 Apr 93 11:58:01 -0400 Date: Wed, 21 Apr 93 11:58:01 -0400 Message-Id: <9304211558.AA07266@cascade> To: nih-image@soils.umn.edu Subject: NU200 Board Has anyone had any experience with the NU200 board? There is some question about its use for digitizing non-NTSC frame formats, provided to it by CCD arrays. - David Salzman From kyzy@rice.edu Wed Apr 21 08:46:02 1993 Received: from moe.rice.edu by nx1.soils.umn.edu (5.65c) id AA12997; Wed, 21 Apr 1993 13:44:42 -0500 Return-Path: Received: from [128.42.105.7] (erinna.rice.edu) by moe.rice.edu (AA20597); Wed, 21 Apr 93 13:46:02 CDT Date: Wed, 21 Apr 93 13:46:02 CDT Message-Id: <9304211846.AA20597@moe.rice.edu> To: From: kyzy@rice.edu Subject: Re: NIH Image and X-Y stage >>Does anybody have a macro that permits NIH Image to work with >>an X-Y stage driver such as an LEP? Here is a different approach that might interest some people. We are using a second Mac to control an X-Y-Z LEP motorized stage. This Mac SE runs a LabView 2.1 program we developed. It can move the stage to several user-specified positions and trigger image acquisition once every move is completed (another Mac running Image acquires the images), it opens and closes motorized diaphragms, opens and closes solenoid valves (gas feed to incubator) and monitors the temperature of the stage incubator. Using two Macs may sound like overkill, but we had an old Mac lying around and the LabView program gives us more flexibility and functionality. ------------------------------------ Prof. Kyriacos Zygourakis Dept. of Chemical Engineering Rice University Houston, Texas 77251-1892 Phone: (713) 527-8750 Ext. 3509 FAX: (713) 285-5478 ------------------------------------ From JONKER@amc.uva.nl Mon Apr 22 10:32:08 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA16208; Thu, 22 Apr 1993 02:31:32 -0500 Return-Path: Received: from HASARA5.BITNET (MAILGATE@HASARA5) by vx.cis.umn.edu (PMDF #2574 ) id <01GXANP29XTS9AOBO3@vx.cis.umn.edu>; Thu, 22 Apr 1993 02:32:48 CDT Received: from SARA.NL by SARA.NL for nih-image@soils.umn.edu; 22 Apr 93 9:32 MET Received: from amc.uva.nl by SARA.NL with PMDF#10201; Thu, 22 Apr 1993 09:32 MET Received: from amc.uva.nl by amc.uva.nl (PMDF #2498 ) id <01GXB2AAI1PC0002HN@amc.uva.nl>; Thu, 22 Apr 1993 09:32:08 MET Date: 22 Apr 1993 09:32:08 +0100 (MET) From: JONKER@amc.uva.nl Subject: Re: NU200 Board To: nih-image@soils.umn.edu Message-Id: <01GXB2AAI1PE0002HN@amc.uva.nl> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Transfer-Encoding: 7BIT Yes, I have used the Nu200 board once to capture images from a Photometrics camera (1315x1017 pixel slow scan CCD camera). It can output either 16 bit propretary format or 8 bit (scaled From the captured 12 or 14 bit values) tiff format. The Received: from hub.ubc.ca by nx1.soils.umn.edu (5.65c) id AA16438; Thu, 22 Apr 1993 03:33:03 -0500 Return-Path: Received: from otter.biochem.ubc.ca by hub.ubc.ca (4.1/1.14) id AA17564; Thu, 22 Apr 93 01:34:22 PDT Received: by otter.biochem.ubc.ca (920330.SGI/920502.SGI.AUTO) for @hub.ubc.ca:nih-image@nx1.soils.umn.edu id AA03204; Thu, 22 Apr 93 01:34:38 -0700 Date: Thu, 22 Apr 1993 01:32:39 -0700 (PDT) From: tom jovin Subject: Re: NU200 Board To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <01GXB2AAI1PE0002HN@amc.uva.nl> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII We too use the Nu200 board routinely with a Photometrics Series 200 camera (large format Kodak chip). There are a few bugs, i.e. external trigger does not work but that may be a problem with firmware revisions. I am not aware that the board is used with cameras from any other company. Without more documentatino, it would be hard to adapt, is my guess. Tom Jovin From geller@synapse.umdnj.edu Thu Apr 22 06:55:31 1993 Received: from synapse.UMDNJ.EDU by nx1.soils.umn.edu (5.65c) id AA17627; Thu, 22 Apr 1993 09:58:34 -0500 Return-Path: Date: Thu, 22 Apr 93 10:55:31 EDT From: "Herbert M. Geller" To: nih-image@soils.umn.edu Subject: Hamamatsu Frame Grabber Message-Id: <9304221055.aa02595@synapse.umdnj.edu> I just spoke to Hamamatsu, located in NJ, about their frame grabber board. It seems like a generic RS-170/CCIR frame grabber, at least from an oral description. I asked the technical person to send me some information and I'll post anything if it seems special, or if there I widespread interest I'll summarize the specs. Herb Geller From geller@synapse.umdnj.edu Thu Apr 22 08:21:14 1993 Received: from synapse.UMDNJ.EDU by nx1.soils.umn.edu (5.65c) id AA18030; Thu, 22 Apr 1993 11:24:16 -0500 Return-Path: Date: Thu, 22 Apr 93 12:21:14 EDT From: "Herbert M. Geller" To: nih-image@soils.umn.edu Subject: Re: Hamamatsu Frame Grabber Message-Id: <9304221221.aa02697@synapse.umdnj.edu> Interestingly, after I posted my information, I got a call back From the Tech Rep, who asked where I heard about the frame grabber. Received: from alsys1.aecom.yu.edu by nx1.soils.umn.edu (5.65c) id AA18925; Thu, 22 Apr 1993 15:01:12 -0500 Return-Path: Received: by alsys1.aecom.yu.edu id AA05865 (5.65c/IDA-1.4.4/AECOM-RIT for nih-image@soils.umn.edu); Thu, 22 Apr 1993 16:02:26 -0400 Date: Thu, 22 Apr 1993 15:59:59 -0400 (EDT) From: Michael Cammer Subject: Ektron 1412 CCD camera To: nih-image@soils.umn.edu In-Reply-To: <9304221221.aa02697@synapse.umdnj.edu> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Does anybody have any experience with this equipment? cammer@aecom.yu.edu From SMIT@CABO.AGRO.NL Tue Apr 23 15:00:00 1993 Received: from hearnvax.nic.surfnet.nl by nx1.soils.umn.edu (5.65c) id AA22493; Fri, 23 Apr 1993 07:08:04 -0500 Return-Path: Received: from AGRO.NL (AGRO02) by HEARNVAX.nic.SURFnet.nl (PMDF #3330 ) id <01GXCQDDOJ8G003O8O@HEARNVAX.nic.SURFnet.nl>; Fri, 23 Apr 1993 14:10:37 MET Received: from CABO.AGRO.NL by AGRO.NL with PMDF#10389; Fri, 23 Apr 1993 14:01 MET Date: 23 Apr 1993 14:00 +0100 (MET) From: "A.L. SMIT" To: nih-image@soils.umn.edu Message-Id: <4897EBD72CDF401251@CABO.AGRO.NL> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: Q_IMAGE X-Vms-Cc: SMIT Content-Transfer-Encoding: 7BIT review From msachs@admin.ogi.edu Fri Apr 23 06:14:09 1993 Received: from ogicse.cse.ogi.edu (cse.ogi.edu) by nx1.soils.umn.edu (5.65c) id AA25185; Fri, 23 Apr 1993 15:13:02 -0500 Return-Path: Received: by ogicse.cse.ogi.edu (/\==/\ Smail3.1.25.1 #25.3) id ; Fri, 23 Apr 93 13:14 PDT Received: from [129.95.74.150] (atalk30.ese.ogi.edu) by admin.ogi.edu.ogi.edu (4.1/SMI-4.1) id AA13636; Fri, 23 Apr 93 13:14:10 PDT Date: Fri, 23 Apr 93 13:14:09 PDT Message-Id: <9304232014.AA13636@admin.ogi.edu.ogi.edu> To: From: msachs@admin.ogi.edu (Matthew Sachs) Subject: Re: >review ?? Recieved message Matthew Sachs From SMIT@CABO.AGRO.NL Fri Apr 26 09:51:00 1993 Received: from hearnvax.nic.surfnet.nl by nx1.soils.umn.edu (5.65c) id AA08591; Mon, 26 Apr 1993 01:50:21 -0500 Return-Path: Received: from AGRO.NL (AGRO02) by HEARNVAX.nic.SURFnet.nl (PMDF #3330 ) id <01GXGM5Y314G00C5LG@HEARNVAX.nic.SURFnet.nl>; Mon, 26 Apr 1993 08:53:08 MET Received: from CABO.AGRO.NL by AGRO.NL with PMDF#10389; Mon, 26 Apr 1993 08:52 MET Date: 26 Apr 1993 08:51 +0100 (MET) From: "A.L. SMIT" Subject: Re: Re: To: nih-image@soils.umn.edu Message-Id: <466792D1F4DF4011D8@CABO.AGRO.NL> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" X-Vms-Cc: SMIT Content-Transfer-Encoding: 7BIT >review ?? Recieved message Matthew Sachs Sorry, my mistake Bert From huff@MCCLB0.MED.NYU.EDU Mon Apr 26 07:30:18 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA10606; Mon, 26 Apr 1993 11:30:11 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXGTQ7J7LS00048S@MCCLB0.MED.NYU.EDU>; Mon, 26 Apr 1993 12:30:19 EDT Date: Mon, 26 Apr 1993 12:30:18 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Very accurate time measurements in Image To: nih-image@soils.umn.edu Message-Id: <01GXGTQ7U71U00048S@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT I have just posted source for updates to 1.49 into /pub/image/contrib on zippy.nimh.nih.gov, file ImageTimer.sit.hqx, which provide the ability to use the time manager to measure elapsed time in microseconds (resolution is around 25 microseconds), and to program a function is user.p which is executed on the interrupt level with very accurate timing (assuming that no other interrupt routines on your Mac are hogging time). These changes also modify the UserCode macro procedure to make unused parameters optional, and to allow the use of a string instead of an integer as the first argument. Also, two new macro FUNCTIONS (not procedures), UserFunc and UserStr, are defined, which return a real and a string result, respectively. Both take one string selector argument and 3 optional numeric parameters. Some sample code to use the time manager is in user.p, and can be invoked From macros via UserCode('alloctimer'), UserCode('starttimer',1.005), etc. Received: from sbms01.msrc.sunysb.edu by nx1.soils.umn.edu (5.65c) id AA12989; Mon, 26 Apr 1993 20:35:00 -0500 Return-Path: Date: Mon, 26 Apr 93 21:36 EDT From: Barr Kum Subject: Single image at a time to VCR to make movie To: nih-image@soils.umn.Edu Message-Id: <45FCB1FB42FFA04C22@SBMS01.MSRC.SUNYSB.EDU> X-Organization: Marine Sciences Research Center X-Envelope-To: nih-image@soils.umn.Edu X-Vms-To: IN%"nih-image@soils.umn.edu" X-Vms-Cc: SBK111 Sometime back I posted a query requesting help on recording a single image at a time onto a VCR. I did recieve replies which are appended at the end. I did manage to make a movie sequence by recording each image in this manner. This method bypasses the memory constraint when using animations on the computer. Equipment used was 1) Mac II cx 2) Syscom Mediator Scan convertor 3) Nec PC VCR (serial port controllable) Software NIH Image 1.49 Multiple images of contour plots were made on a workstation saved in Tiff format and telneted onto the Mac. Caution if you can generate PICT files use them as they use up less space. Out of the 600 images generated there was space for only 250 on a disk with 80 MB free space. The VCR was hooked up to the video output via scan convertor. Another line connecting VCR serial port and Mac serial was used to control VCR actions from the Mac. A macro written in NIH image was then used to crontrol the action of the VCR. Each image w called up. The menu bar etc were visible in the movie. Wayne Rasband has said that he will consider having a Macro call for PHOTOMODE. This should solve that problem. For some reason if a Pause command was sent to the VCR insted of a STOP the syncronisation got mixed up. Recording would occur when the image was being closed etc. Ive given the Macro used below. The general outline of the macro is as follows 1) Call up a image file 2) Send message to VCR via serial port to start recording 3) Time a sec (or any other) 4) Send message to VCR to stop recording 5) close file, loop What is needed is to have photomode between 1 and 2, where the whole Image fills the screen. I had to do this thru a macro as i was sending 250 images to the VCR. However f you are sending only a couple you could load the image, go into photomode and have a macro that will can be activated by command keys to send message to VCR to start recording and stop after fixed time. Hope this helps Sereno Barr-Kumarakulasinghe Marine Sciences Research Center SUNY @ Stony Brook NY 11794-5000 e-mail: sbarrkum@csserv1.ic.sunysb.edu sbk111@msrc.sunysb.edu ------------------------------------------------------------------ macro 'FileToVCR'; var linefeed,return,r,c,p,s,t,crlf,rc, ps,st,file:string; i:integer; begin r:=chr(82); c:=chr(67); p:=chr(80); s:=chr(83); t:=chr(84); linefeed:=chr(10); return:=chr(13); crlf:=concat(return,linefeed); rc:=concat(r,c,crlf); ps:=concat(p,s,crlf) st:=concat(s,t,crlf) RequiresVersion(1.48); OpenSerial('1200 baud,no parity,eight data,one stop'); for i:=1 to 275 do begin SetImport('TIFF'); Import('Temt', i:3) {photo Mode should be included here when available} PutSerial(rc); Wait(4); PutSerial(st); Wait(3);} close; Beep; end end; ----------------------------------------------------------- From: mvivino@helix.nih.gov Barr Kum writes: >I need to know of a way to write images from a window to a videotape on a >VCR. We've got a NEC VCR that is supposed to be capable of advancing frame >by frame. You have a number of options, depending on your budget and requirements you might be able to do the job with next to nothing, or you might need fairly big bucks. Option 1) Get access to a quadra. The quadra has built in video. You will need to make or buy a cable that goes from the quadra to BNC. Read trhe document: quadra_video.txt in /pub/image/documents of zippy.nimh.nih.gov. I might also be able to send you a schematic. YOU ****MUST**** USE APPLE CONVOLUTION MODE in the monitors control panal to get decent video. You will only get grayscale. Option 2) (not recommended) Use a RasterOps video expander. Its cheap (and its also inexpensive). It does a worse job than the quadra video. Option 3) Buy a radius video vision, get color and decent video. High cost. For all the above options you can use the serial port macro calls to advance your vcr, if the vcr is that "smart" a vcr. Mark ---------------------------------------------------------------------------- From: kartenh@sdsc.edu Another option is the Scion NTSC output card, for about $595.?[ --------------------------------------------------------------------------- From: D1887@AppleLink.Apple.Com FROM: Bonnie Polesky D1887 SUB: Mac to VCR Dear Mr. Kum: In response to your inquiry of writing images from a Mac window to a VCR, Scion Corporation offers a product, the TV-3, which is capable of outputing grayscale images from a version of NIH Image in just this way. The TV-3 converts the image contained in its memory buffer into an RS-170 video signal for outputting to devices such as video tape recorders and video printers. The TV-3 sells for $595 and is available direct from Scion. If you wish to receive literature on the TV-3, please forward your address and fax number to us. Please feel free to contact us if you have any questions. Sincerely, Bonnie Polesky Director, Sales & Marketing =============================================================================== From: IN%"mvivino@helix.nih.Gov" 8-APR-1993 15:18:16.84 Subj: RE: Mac to VCR (using a quadra 950) >From what youve said and going thru the document quadra..at zippy... it looks >like color output can be written onto video-tape. However how do I get about >sending only what is in a window to the vcr as against the whole screen. >Any advice really welcome. I was pretty sure it was only grayscale. What you need to do is first make the cable. I have uploaded a schematic to zippy.nimh.nih.gov in the pub/image/contrib directory. Then to output just the image you can do one of two things: 1) Have only the cable running as video and load the image into the Image program. Then select Photomode. 2) Use two monitors, one from the cable the other from a built in video card (use this as monitor one). Then make your image scaled as large as monitor two. Mark ============================================================================ From wayne@zippy.nimh.nih.gov Tue Apr 27 17:02:26 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA17473; Tue, 27 Apr 1993 17:02:40 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA19294; Tue, 27 Apr 93 18:13:28 -0400 Message-Id: <9304272213.AA19294@zippy.nimh.nih.gov> Date: Tue, 27 Apr 1993 17:02:26 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: 1.50 beta with plug-in support Cc: wayne@zippy.nimh.nih.gov I have uploaded a new beta version of Image to zippy.nimh.nih.gov that features support for Photoshop compatible acquisition plug-ins. Plug-in are accessed using a new command in the File menu called Acquire. I have successfully tested plug-ins for the LaCie SilverScanner, the Kodak DCS-200 digital camera, and the Perceptics PixelBuffer frame grabber. The PixelBuffer plug-in is available from the new plug-ins directory on Zippy. It was contributed, including C source, by Paul Whalen of Perceptics. Image can acquire 8-bit grayscale, 8-bit indexed color, and 24-bit color images via plug-ins. 24-bit color images are stored in three slice stacks and displayed using 8-bit indexed color, similar to the way the Capture Color command works. V1.50 also has a new macro command for supporting plug-ins. It has the form Acquire('plug-in name'). Full path names such as 'HD400:Plug-ins:Kodak DCS-200' are allowed. V1.50 also fixes a problem that sometimes caused the Skeletonize routine to never complete. --wayne From roper@lamar.ColoState.EDU Tue Apr 27 11:25:35 1993 Received: from lamar.ColoState.EDU by nx1.soils.umn.edu (5.65c) id AA18479; Tue, 27 Apr 1993 18:24:10 -0500 Return-Path: Received: from [129.82.126.25] by lamar.ColoState.EDU (AIX 3.2/UCB 5.64/4.03) id AA60473; Tue, 27 Apr 1993 17:25:35 -0600 Date: Tue, 27 Apr 1993 17:25:35 -0600 Message-Id: <9304272325.AA60473@lamar.ColoState.EDU> To: From: roper@lamar.ColoState.EDU Subject: Re: 1.50 beta with plug-in support The news about acquiring images from the Kodak DCS 200 camera into Image 1.50 beta is great news! I am using a Kodak DCS 200 and am VERY impressed with the images. I import images into PageMaker, add labels, arrows, asterisks etc, and FTP the final figure to our campus color (Tektronix Phaser IISD) or B/W (linotronics) printer, a few buildings away. I can produce publication-quality figures in minutes rather than days via conventional printing and rub-on lettering (about half of which get rubbed off!). And it's cheaper (~$3/ 8x10 print). The only drag is that the Kodak DCS 200 does not yet fit directly onto my Zeiss Axiophot. The Kodak DCS 200 uses a Nikon bayonet mount. And Zeiss charges an arm and a leg for the appropriate adapters to get the Kodak camera to fit onto their 'scope. Does anyone have experience with Zeiss's ZVS Video camera system that is built around an Optronics LX-450A RGB remote-head microscope camera? I was impressed with its ability to acquire bright and low light level images (e.g., immunocytochemistry). At $7K, it is about halfway in cost between a Sony color video camera (not anywhere near as sensitive) and SIT or cooled CCD cameras. I'd like some seasoned advice prior to laying down the bucks, though. -Steve Roper -------------------------------------------- Stephen Roper, Professor of Anatomy and Neurobiology, Colorado State University, Ft. Collins CO 80523 INTERNET: Roper@Lamar.ColoState.Edu FAX:(303) 491-7907 PHONE: (303) 491-7808 From tom@otter.biochem.ubc.ca Tue Apr 27 10:48:11 1993 Received: from hub.ubc.ca by nx1.soils.umn.edu (5.65c) id AA19100; Tue, 27 Apr 1993 19:48:06 -0500 Return-Path: Received: from otter.biochem.ubc.ca by hub.ubc.ca (4.1/1.14) id AA05369; Tue, 27 Apr 93 17:49:28 PDT Received: by otter.biochem.ubc.ca (920330.SGI/920502.SGI.AUTO) for @hub.ubc.ca:nih-image@soils.umn.edu id AA21560; Tue, 27 Apr 93 17:49:47 -0700 Date: Tue, 27 Apr 1993 17:48:11 -0700 (PDT) From: tom jovin Subject: Re: 1.50 beta with plug-in support To: nih-image@soils.umn.edu In-Reply-To: <9304272325.AA60473@lamar.ColoState.EDU> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII re Steve Roper's request about cameras: You may wisht o look into the Microimager made by Xillix in Vancouver. 10 and 12-bit versions using the large Kodak chip. Operates at up to 8 Megapixels/s readout rate with very good dynamic range and SNR. tom jovin From crc@leicester.ac.uk Wed Apr 28 04:18:44 1993 Received: from sun2.nsfnet-relay.ac.uk by nx1.soils.umn.edu (5.65c) id AA23293; Wed, 28 Apr 1993 04:18:44 -0500 Return-Path: Via: uk.ac.leicester; Wed, 28 Apr 1993 10:19:36 +0100 Received: from irix.le.ac.uk by xtelpp.leicester.ac.uk with SMTP (PP) id <09479-0@xtelpp.leicester.ac.uk>; Wed, 28 Apr 1993 10:16:12 +0100 From: "Dr C. Cane" Message-Id: <27115.9304280918@irix.le.ac.uk> Subject: Re: Zeiss Camera To: nih-image@soils.umn.edu Date: Wed, 28 Apr 93 10:18:53 BST In-Reply-To: <9304272325.AA60473@lamar.ColoState.EDU>; from "roper" at Apr 27, 93 6:25 pm X-Mailer: ELM [version 2.3 PL0 (LUT)] We had a Zeiss RGB camera on demo but wern't very impressed. It was not as sensitive as we hoped. If you need multicolour and sensitivity the only way to go is to get a SIT or cooled CCD and capture one image for each colour then superimpose them using Image or Photoshop (I find this better as it gives more control over colour balance & you can easily boost eg. red relative to green). Ive got a Hamamatsu C2400 SIT camera which is pre-processed by an Argus 10 before capturing the image on the Mac. I find this set-up sensitive enough for my applications, and routinely use image superimposition to do multimode imaging. I hope these comments are useful. Chris Cane, Dept of Biochemistry, University of Leicester, Leicester, LE1 7RH., UK. Tel +44 533 523480 Fax +44 533 523369. From roper@lamar.ColoState.EDU Wed Apr 28 03:23:25 1993 Received: from lamar.ColoState.EDU by nx1.soils.umn.edu (5.65c) id AA26306; Wed, 28 Apr 1993 10:22:01 -0500 Return-Path: Received: from [129.82.126.25] by lamar.ColoState.EDU (AIX 3.2/UCB 5.64/4.03) id AA32708; Wed, 28 Apr 1993 09:23:25 -0600 Date: Wed, 28 Apr 1993 09:23:25 -0600 Message-Id: <9304281523.AA32708@lamar.ColoState.EDU> To: From: roper@lamar.ColoState.EDU Subject: Re: Zeiss Camera Chris Thanks for the feedback. Do you recall about how much you paid for the Hamamatsu SIT camera? -Steve -------------------------------------------- Stephen Roper, Professor of Anatomy and Neurobiology, Colorado State University, Ft. Collins CO 80523 INTERNET: Roper@Lamar.ColoState.Edu FAX:(303) 491-7907 PHONE: (303) 491-7808 From hart@actin.rutgers.edu Tue Apr 27 08:33:22 1993 Received: from actin.rutgers.edu by nx1.soils.umn.edu (5.65c) id AA26567; Wed, 28 Apr 1993 10:56:10 -0500 Return-Path: Received: by actin.rutgers.edu (5.59/25-eef) id AA04247; Tue, 27 Apr 93 12:33:22 EDT Date: Tue, 27 Apr 93 12:33:22 EDT From: Ronald P. Hart Message-Id: <9304271633.AA04247@actin.rutgers.edu> To: nih-image@soils.umn.edu help From huff@MCCLB0.MED.NYU.EDU Thu Apr 29 04:04:29 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA02673; Thu, 29 Apr 1993 08:03:28 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXKTF2JX80000BHD@MCCLB0.MED.NYU.EDU>; Thu, 29 Apr 1993 09:04:30 EDT Date: Thu, 29 Apr 1993 09:04:29 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Straw Poll: Create comp.sys.mac.scitech? To: nih-image@soils.umn.edu Message-Id: <01GXKTF2TASY000BHD@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT There is currently a discussion in news.groups regarding the possible creation of a Usenet newsgroup comp.sys.mac.scitech. Generally, the opponents of the group point out that there already exists a group sci.comp-aided which has essentially no traffic, and should be used instead. As I recall, the proposers will archive the group and include an archive of software. The RFD has expired at my Usenet server, so I can't include a copy. I will post a request that it be reposted. Interested readers of the NIH Image mailing list, please answer this poll by entering Y or N at the end of each line: Read some Usenet groups Read sci.image.processing Read comp.sys.mac.programmer Read comp.sys.mac.applications Would read comp.sys.mac.scitech Would read sci.comp-aided if it had traffic Would vote on the comp.sys.mac.scitech CFV If so, how would you vote? -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From WETZEL@metro.bst.rochester.edu Thu Apr 29 05:48:00 1993 Received: from metro.bst.rochester.edu by nx1.soils.umn.edu (5.65c) id AA03036; Thu, 29 Apr 1993 08:47:15 -0500 Return-Path: Received: from METRO by METRO (PMDF #12506) id <01GXKUZ852M899DKN4@METRO>; Thu, 29 Apr 1993 09:48 EDT Date: Thu, 29 Apr 1993 09:48 EDT From: WETZEL@metro.bst.rochester.edu Subject: Re: Straw Poll: Create comp.sys.mac.scitech? To: nih-image@soils.umn.edu Message-Id: <01GXKUZ852M899DKN4@METRO> X-Vms-To: IN%"nih-image@soils.umn.edu" Read some Usenet groups: y Read sci.image.processing: no Read comp.sys.mac.programmer: no Read comp.sys.mac.applications: yes Would read comp.sys.mac.scitech: yes Would read sci.comp-aided if it had traffic: no Would vote on the comp.sys.mac.scitech CFV: yes If so, how would you vote?: yes From lake@rcw004.dnet.bp.com Thu Apr 29 05:26:41 1993 Received: from rcwgwy.bp.com by nx1.soils.umn.edu (5.65c) id AA03136; Thu, 29 Apr 1993 08:51:29 -0500 Return-Path: Received: by rcwgwy.bp.com (5.57/Ultrix3.0-C) id AA10546; Thu, 29 Apr 93 09:53:59 -0400 Received: from RCW004.DECnet MAIL11D_V3 by rcwi01.rcw.bp.com (5.57/Ultrix3.0-C) id AA01141; Thu, 29 Apr 93 09:26:42 -0400 Date: Thu, 29 Apr 93 09:26:41 -0400 Message-Id: <9304291326.AA01141@rcwi01.rcw.bp.com> From: lake@rcw004.dnet.bp.com To: nih-image@soils.umn.edu Subject: RE: Straw Poll: Create comp.sys.mac.scitech? From: RCWI01::"nih-image@soils.umn.edu" 29-APR-1993 09:07:35.21 To: Multiple recipients of list CC: Subj: Straw Poll: Create comp.sys.mac.scitech? There is currently a discussion in news.groups regarding the possible creation of a Usenet newsgroup comp.sys.mac.scitech. Generally, the opponents of the group point out that there already exists a group sci.comp-aided which has essentially no traffic, and should be used instead. As I recall, the proposers will archive the group and include an archive of software. The RFD has expired at my Usenet server, so I can't include a copy. I will post a request that it be reposted. Interested readers of the NIH Image mailing list, please answer this poll by entering Y or N at the end of each line: Read some Usenet groups Y Read sci.image.processing Y Read comp.sys.mac.programmer N Read comp.sys.mac.applications SOMETIMES Would read comp.sys.mac.scitech Y Would read sci.comp-aided if it had traffic ? Would vote on the comp.sys.mac.scitech CFV Y If so, how would you vote? Y -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 ================== RFC 822 Headers ================== Received: by rcwgwy.bp.com (5.57/Ultrix3.0-C) id AA10364; Thu, 29 Apr 93 09:08:21 -0400 Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012bdfd2fa011452; Thu, 29 Apr 93 08:07:06 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA02774; Thu, 29 Apr 1993 08:05:19 -0500 Date: Thu, 29 Apr 1993 08:05:19 -0500 Message-Id: <01GXKTF2TASY000BHD@MCCLB0.MED.NYU.EDU> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From janning@vnwz01.uni-muenster.de Thu Apr 29 17:55:48 1993 Received: from VNWZ01.UNI-MUENSTER.DE by nx1.soils.umn.edu (5.65c) id AA03175; Thu, 29 Apr 1993 08:54:51 -0500 Return-Path: Received: by vnwz01.uni-muenster.de (MX V3.1C) id 3830; Thu, 29 Apr 1993 15:55:50 +0200 Date: Thu, 29 Apr 1993 15:55:48 +0200 From: "W. Janning, Allgemeine Zoologie & Genetik, Muenster" To: nih-image@soils.umn.edu Cc: janning@vnwz01.uni-muenster.de Message-Id: <0096BC2F.37599160.3830@vnwz01.uni-muenster.de> Subject: RE: Straw Poll: Create comp.sys.mac.scitech? Read some Usenet groups: N Read sci.image.processing Read comp.sys.mac.programmer Read comp.sys.mac.applications Would read comp.sys.mac.scitech Would read sci.comp-aided if it had traffic Would vote on the comp.sys.mac.scitech CFV: N If so, how would you vote? From John_Mansfield@mse.engin.umich.edu Thu Apr 29 09:04:00 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA03249; Thu, 29 Apr 1993 09:04:00 -0500 Return-Path: Message-Id: <199304291404.AA03249@nx1.soils.umn.edu> Date: 29 Apr 1993 09:52:04 U From: "John Mansfield" Subject: Straw Poll replies To: nih-image@soils.umn.edu Reply_ Straw Poll replies Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu I THINK that Ed maybe wants these replies sent to him and not the whole mailing list? I think that would be more efficient in the long run. Jfm. From John_Mansfield@mse.engin.umich.edu Thu Apr 29 09:09:49 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA03369; Thu, 29 Apr 1993 09:09:49 -0500 Return-Path: Message-Id: <199304291409.AA03369@nx1.soils.umn.edu> Date: 29 Apr 1993 10:10:58 U From: "John Mansfield" Subject: Image FFT To: "NIH Image Mailing List" John Mansfield North Campus Electron Microbeam Analysis Lab University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John.F.Mansfield@umich.edu Time: 10:04 Date:4/29/93 NC EMAL Subject: Image FFT The FHT modifications to Image that Arlo Reeves did a few years ago have rpoved invaluable to us. We incorporated them into our own version of Image that measured the thickness of TEM samples by a diffraction technique (CBED). Whenever Wayne came out with a new version we tried to make sure our changes could be incorporated. That worked fine from version 1.28 to 1.35 but we couldnt get 1.36 to work. The guy who was working with me and was a better programmer than I graduated and so we have never upgraded our code past 1.35. We would however, like the functionality of the later versions. I thought I saw a version of 1.47 or 9 that had the FHT stuff included in it? Is the source available for that? It was the FHT stuff that was giving us the most problems in the upgrade since we didnt originally code that!! I would like to try and get Microscopy/FFT Image working with version 1.50 if possible. So in summary, if you know of the whereabouts of the source code for Arlo's revisions modified for version 1.4 or above please let me know. ADVthanks a bunchANCE John Mansfield. From wayne@zippy.nimh.nih.gov Thu Apr 29 09:13:24 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA03416; Thu, 29 Apr 1993 09:13:52 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA21654; Thu, 29 Apr 93 10:24:29 -0400 Message-Id: <9304291424.AA21654@zippy.nimh.nih.gov> Date: Thu, 29 Apr 1993 09:13:24 +0000 To: root@physix.mta.ca (Stewart Walker) From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: 1.50 beta & RasterOps with plug-in support Cc: nih-image@soils.umn.edu >Tried out the image 1.50b32 beta from zippy with the Rasterops 24STV and 2 >monitors, one at 24 bit colour, the other at 8 bit grey. The PhotoShop >plug-in window comes up and the buttons to adjust PAL,NTSC,SECAM, & colour >work. If I select 640 x 480 or larger size, the setting takes place, and >the black elements of the dialog box overlay the picture,but then the >buttons stop functioning and I get beeps wherever I click. The returnkey >still activates Grab. > >No matter what format the image is in, when I activate grab, the program >hangs after opening an appropriately-sized window called RGB 3/3 on the 256 >grey level monitor. the words repeat loop appear in the values window. I >kill the program with command-option-esc. I am buying a Rasterops 24STV card and expect to be able to fix this bug when I have the card and I am able to do some testing. --wayne From John_Mansfield@mse.engin.umich.edu Thu Apr 29 09:25:07 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA03543; Thu, 29 Apr 1993 09:25:07 -0500 Return-Path: Message-Id: <199304291425.AA03543@nx1.soils.umn.edu> Date: 29 Apr 1993 10:25:12 U From: "John Mansfield" Subject: 1.50 beta & Photo-CD plug-i To: nih-image@soils.umn.edu Reply_ 1.50 beta & Photo-CD plug-in Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu I tried out Image 1.50beta32 with the Photo-CD plugin and get an alert that says that the plug-in requires Photoshop 2.0. Too bad :-(. From wayne@zippy.nimh.nih.gov Thu Apr 29 09:32:32 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA03617; Thu, 29 Apr 1993 09:32:47 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA21700; Thu, 29 Apr 93 10:43:37 -0400 Message-Id: <9304291443.AA21700@zippy.nimh.nih.gov> Date: Thu, 29 Apr 1993 09:32:32 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: 1.50 beta & Photo-CD plug-i >I tried out Image 1.50beta32 with the Photo-CD plugin and get an alert that >says that the plug-in requires Photoshop 2.0. Too bad :-(. I will ask my contact at Kodak how the Photo-CD plug-in it knows what version of Photoshop it's talking to and why it thinks it needs Photoshop 2.0. --wayne From wayne@zippy.nimh.nih.gov Thu Apr 29 09:40:59 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA03726; Thu, 29 Apr 1993 09:41:15 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA21713; Thu, 29 Apr 93 10:52:04 -0400 Message-Id: <9304291452.AA21713@zippy.nimh.nih.gov> Date: Thu, 29 Apr 1993 09:40:59 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Image FFT Perceptics added Arlo Reeves' FFT routines to Image/VDM 1.44, which is available from zippy.nimh.nih.gov, in the image-spinoffs directory. The source code, however, is not on Zippy, but perhaps someone can talk them into making it available. The main problem with Arlo's FFT code is that it is written in assembler, and 68000 assembler code doesn't seem to have much of a future with the Power/PC coming. --wayne From dlange@cern.ce.uiuc.edu Thu Apr 29 15:46:20 1993 Received: from cern04.ce.uiuc.edu (cern11.ce.uiuc.edu) by nx1.soils.umn.edu (5.65c) id AA03780; Thu, 29 Apr 1993 09:49:03 -0500 Return-Path: Received: by cern04.ce.uiuc.edu (1.37.109.4/16.2) id AA03449; Thu, 29 Apr 93 09:48:01 -0500 Date: Thu, 29 Apr 1993 09:46:20 +0600 (CST) From: "David A. Lange" Subject: RE: Straw Poll: Create comp.sys.mac.scitech? To: nih-image@soils.umn.edu In-Reply-To: <9304291326.AA01141@rcwi01.rcw.bp.com> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Read some Usenet groups Y Read sci.image.processing Y Read comp.sys.mac.programmer N Read comp.sys.mac.applications N Would read comp.sys.mac.scitech Y Would read sci.comp-aided if it had traffic ? maybe Would vote on the comp.sys.mac.scitech CFV Y If so, how would you vote? Y ------------------------------------------------------------------------ David A. Lange Department of Civil Engineering University of Illinois Email: dlange@cern.ce.uiuc.edu 2207 NCEL, MC-250 Phone: 217-333-4816 Urbana, IL 61801-2352 ------------------------------------------------------------------------ From afruppe@pxdvgx.erenj.com Thu Apr 29 04:57:08 1993 Received: from erenj.com (ereapp.erenj.com) by nx1.soils.umn.edu (5.65c) id AA03866; Thu, 29 Apr 1993 09:51:41 -0500 Return-Path: Received: by erenj.com (5.65/mjr-920806); id AA24759; Thu, 29 Apr 93 10:53:03 -0400 Received: by eredns.erenj.com (5.65/bdb-mailv1.2-erenj-gw); id AA19043; Thu, 29 Apr 93 10:53:00 -0400 Posted-Date: Thu, 29 Apr 1993 09:57:08 -0500 Message-Id: <9304291453.AA19043@eredns.erenj.com> Date: Thu, 29 Apr 1993 09:57:08 -0500 To: nih-image@soils.umn.edu From: afruppe@pxdvgx.erenj.com >Read some Usenet groups Y >Read sci.image.processing Y >Read comp.sys.mac.programmer N >Read comp.sys.mac.applications N >Would read comp.sys.mac.scitech N >Would read sci.comp-aided if it had traffic >Would vote on the comp.sys.mac.scitech CFV N >If so, how would you vote? =========================================================================== All thoughts expressed are mine and are not intended to reflect the opinions of my employer. (disclaimer) Al Ruppert afruppe@pxdvgx.erenj.com (908) 730-2979 From John_Mansfield@mse.engin.umich.edu Thu Apr 29 09:52:11 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA03875; Thu, 29 Apr 1993 09:52:11 -0500 Return-Path: Message-Id: <199304291452.AA03875@nx1.soils.umn.edu> Date: 29 Apr 1993 10:52:59 U From: "John Mansfield" Subject: Re: Image FFT To: nih-image@soils.umn.edu Reply_ RE>>Image FFT Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu The good thing about the PowerPC is that we should be able to use routines like the 2D FFT from Numerical Recipes and get quite good turnaround times!! I was trying to get Apple to let us try out one of the New Cyclone machines and try and use the DSP to do FFTs fast, but they seem to be ignoring my request. Anyone tried one out? Wayne, what is the future for Image in the PowerPC world? Are you going to port it? I guess you have to buy an RS/6000 at the moment to try compiling code! -------------------------------------- Date: 4/29/93 10:44 To: John Mansfield From: nih-image@soils.umn.edu Perceptics added Arlo Reeves' FFT routines to Image/VDM 1.44, which is available from zippy.nimh.nih.gov, in the image-spinoffs directory. The source code, however, is not on Zippy, but perhaps someone can talk them into making it available. The main problem with Arlo's FFT code is that it is written in assembler, and 68000 assembler code doesn't seem to have much of a future with the Power/PC coming. --wayne ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;29 Apr 1993 10:44:23 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012bdfe97b012672; Thu, 29 Apr 93 09:43:08 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA03749; Thu, 29 Apr 1993 09:41:29 -0500 Date: Thu, 29 Apr 1993 09:41:29 -0500 Message-Id: <9304291452.AA21713@zippy.nimh.nih.gov> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: wayne%zippy.nimh.nih.gov@relay.tc.umn.edu (Wayne Rasband) To: Multiple recipients of list Subject: Re: Image FFT From huff@MCCLB0.MED.NYU.EDU Thu Apr 29 05:56:09 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA03921; Thu, 29 Apr 1993 09:55:03 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXKXBIWEOW000HVP@MCCLB0.MED.NYU.EDU>; Thu, 29 Apr 1993 10:56:10 EDT Date: Thu, 29 Apr 1993 10:56:09 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Re: Image FFT To: nih-image@soils.umn.edu Message-Id: <01GXKXBJ3WR6000HVP@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >We would however, like the functionality of the later versions. I thought I >saw a version of 1.47 or 9 that had the FHT stuff included in it? Is the >source available for that? It was the FHT stuff that was giving us the most >problems in the upgrade since we didnt originally code that!! I would like to >try and get Microscopy/FFT Image working with version 1.50 if possible. >So in summary, if you know of the whereabouts of the source code for Arlo's >revisions modified for version 1.4 or above please let me know. Try looking for IMAGE VDM on zippy. I believe it has the source. If it doesn't, let me know. I have the source and will post it. Many thanks to Paul Whalen of Perceptics for making this available. (The file on zippy is the self extracting archive I received on a disk from Paul. I haven't actually extracted it to see what was in there, but an earlier version included the source). The label on the outside of the floppy says "1.44". BTW, this is a BITNET LISTSERV list, and one of the annoying properties of LISTSERV is that it sets the REPLY TO header to the list, so that a "reply" command in e.g. Eudora sets the "to" field to the list. Everyone should try to remember that some mailing lists do this, others don't. I was going to send this reply to just John Mansfield but it is probably of general interest. -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From jladwig@soils.umn.edu Thu Apr 29 05:18:33 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA04104; Thu, 29 Apr 1993 10:18:11 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA15667; Thu, 29 Apr 93 10:18:33 CDT Date: Thu, 29 Apr 93 10:18:33 CDT From: "John Ladwig" Message-Id: <9304291518.AA15667@saturn.soils.umn.edu> To: Subject: LISTSERV software and replies (administrivia) [ was Re: Image FFT] In-Reply-To: Edward J. Huff's message <01GXKXBJ3WR6000HVP@MCCLB0.MED.NYU.EDU> of 29 April 1993 References: <01GXKXBJ3WR6000HVP@MCCLB0.MED.NYU.EDU> Edward J. Huff writes on 29 April 1993 at 10:01:59 -0500 > > BTW, this is a BITNET LISTSERV list, and one of the annoying properties of > LISTSERV is that it sets the REPLY TO header to the list, so that a "reply" > command in e.g. Eudora sets the "to" field to the list. Everyone should > try to remember that some mailing lists do this, others don't. I was going > to send this reply to just John Mansfield but it is probably of general > interest. It is of general interest, and I'm looking into ways to hack the software into defaulting to private reply, with CC: to the list. There is a glitch in this, however, as some mail user agents don't have a "Reply to all" command, so a lot of replies to public messages would only go privately. Even if the user agent does have the command, a lot of folks don't use it, and even more replies go by private mail. This is a classic tradeoff, and it's currently biased in favor of keeping message traffic public. Thus the few public replies to an essentially private poll today. This hasn't really been a problem to date, and so I haven't lookd into changing the behavior of the software very seriously. BTW - this is *not* a BITNET LISTSERV list, it's run using Unix-listserv from Boston University, and runs on a NeXT. -jml NIH-Image mailing list administrator -- UMN Department of Soil Science; St. Paul, MN Internet: john.ladwig@soils.umn.edu Fidonet: John Ladwig 1:282/341 jladwig@torpedo.forestry.umn.edu From ltague@physio1.utmem.edu Thu Apr 29 05:23:34 1993 Received: from physio1.utmem.edu by nx1.soils.umn.edu (5.65c) id AA04196; Thu, 29 Apr 1993 10:22:10 -0500 Return-Path: Received: by physio1.utmem.edu (5.57/Ultrix3.0-C) id AA18856; Thu, 29 Apr 93 10:23:35 -0500 Message-Id: <9304291523.AA18856@physio1.utmem.edu> To: Cc: Multiple recipients of list Subject: Re: Straw Poll: Create comp.sys.mac.scitech? In-Reply-To: Your message of "Thu, 29 Apr 93 08:04:27 CDT." <01GXKTF2TASY000BHD@MCCLB0.MED.NYU.EDU> Date: Thu, 29 Apr 93 10:23:34 -0500 From: ltague@physio1.utmem.edu X-Mts: smtp PLEASE provide a utility which can be used by those who would like to access a "Usenet news" type discussion to nih-image and those who those who would only like to access the information that is being discussed in a simple straight forward manner and maybe do a keyword search. The answer would be a gopher server with one of its items pointing at nih-image news reader. This will provide something that is not currently being provided. Answers to your questions are below: Read some Usenet groups yes Read sci.image.processing no Read comp.sys.mac.programmer no Read comp.sys.mac.applications no Would read comp.sys.mac.scitech yes Would read sci.comp-aided if it had traffic no Would vote on the comp.sys.mac.scitech CFV yes If so, how would you vote? yes... if thats all that can be done?? Thanks for all the good work on nih-image. Have a nice day! Larry Larry Tague U.T., Memphis Research Associate Dept. of Physiology & Biophysics Research Program Director, MECCA* 894 Union Ave Phone Bus.: 901-528-7152 Memphis, TN 38163 Phone FAX: 901-528-7126 e-mail: ltague@physio1.utmem.edu *MECCA (Memphis Educational Computer Connectivity Alliance) -------- From wayne@zippy.nimh.nih.gov Thu Apr 29 10:25:40 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA04221; Thu, 29 Apr 1993 10:25:56 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA21788; Thu, 29 Apr 93 11:36:46 -0400 Message-Id: <9304291536.AA21788@zippy.nimh.nih.gov> Date: Thu, 29 Apr 1993 10:25:40 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Image FFT and PowerPC >Wayne, what is the future for Image in the PowerPC world? >Are you going to port it? I guess you have to buy an RS/6000 at the >moment to try compiling code! I will port Image to the PowerPC when there is a PowerPC Pascal compiler that runs in the Mac environment. No way would I buy an RS/6000 just to port Image. Does anyong know if Symantec is coming out with a PowerPC version of Think Pascal? --wayne From huff@MCCLB0.MED.NYU.EDU Thu Apr 29 07:24:54 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA04711; Thu, 29 Apr 1993 11:25:53 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXL0FJW0XC000IUI@MCCLB0.MED.NYU.EDU>; Thu, 29 Apr 1993 12:24:55 EDT Date: Thu, 29 Apr 1993 12:24:54 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: RE: Image FFT To: nih-image@soils.umn.edu Message-Id: <01GXL0FK4V82000IUI@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >Perceptics added Arlo Reeves' FFT routines to Image/VDM 1.44, which is >available from zippy.nimh.nih.gov, in the image-spinoffs directory. The >source code, however, is not on Zippy, but perhaps someone can talk them >into making it available. The main problem with Arlo's FFT code is that it >is written in assembler, and 68000 assembler code doesn't seem to have much >of a future with the Power/PC coming. > >--wayne I just spoke to Paul Whelan and got his permission to post the source. It will be in contrib, and no doubt Wayne will move it to spinoffs. (I ran the self extracting archive and was restuffing it with Stuffit Lite, which crashed twice. So I searched my comp.sys.mac.* archive and saw that Stuffit Lite has a random crash problem. Back to Stuffit Classic...) -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From salzman@Athena.MIT.EDU Thu Apr 29 08:38:10 1993 Received: from Athena.MIT.EDU (ATHENA-AS-WELL.MIT.EDU) by nx1.soils.umn.edu (5.65c) id AA04827; Thu, 29 Apr 1993 11:36:49 -0500 Return-Path: Received: from CASCADE.MIT.EDU by Athena.MIT.EDU with SMTP id AA21220; Thu, 29 Apr 93 12:38:13 EDT From: salzman@Athena.MIT.EDU Received: by cascade (5.57/4.7) id AA22922; Thu, 29 Apr 93 12:38:10 -0400 Date: Thu, 29 Apr 93 12:38:10 -0400 Message-Id: <9304291638.AA22922@cascade> To: nih-image@soils.umn.edu Subject: FFT Please, please, please do *not* add machine-dependent FFT code to your version of Image, or to the master copy. Rather, use the carefully maintained and heavily optimized version available from LAPACK. LAPACK is a public domain library of mathematical routines maintained by the numerical analysis community. Converting FORTRAN rountines to Think Pascal is easy, but keeping up with new hardware-software interactions (e.g. a new cache size) is hard. For further information, send E_Mail to netlib@surfer.epm.ornl.gov with a blank Subject line and the words "send index" as the first line of the file. - David Salzman From jladwig@soils.umn.edu Thu Apr 29 09:37:17 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA05869; Thu, 29 Apr 1993 14:36:56 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA16624; Thu, 29 Apr 93 14:37:17 CDT Date: Thu, 29 Apr 93 14:37:17 CDT From: "John Ladwig" Message-Id: <9304291937.AA16624@saturn.soils.umn.edu> To: Subject: Re: Straw Poll: Create comp.sys.mac.scitech? In-Reply-To: ltague@physio1.utmem.edu's message <9304291523.AA18856@physio1.utmem.edu> of 29 April 1993 References: <9304291523.AA18856@physio1.utmem.edu> ltague@physio1.utmem.edu writes on 29 April 1993 at 10:26:05 -0500 > PLEASE provide a utility which can be used by those who would like to > access a "Usenet news" type discussion to nih-image and those who those > who would only like to access the information that is being discussed > in a simple straight forward manner and maybe do a keyword search. The > answer would be a gopher server with one of its items pointing at > nih-image news reader. This will provide something that is not > currently being provided. I haven't done Usenet news previously because we are volunteering the support for this mailing list, and it seemed like more work than I wanted to undertake. Running a vote takes a fair amount of effort, and there's no guarantee that it'll pass. Short of a sci. or comp. newsgroup, one *could* set a new group up in the alt. hierarchy, but many sites don't get a full feed. A mailing list is something which can be done fairly simply, and can provide very low common-denominator transmission of information. In regards to gopher access, it's been up since early March. A posting went out to the list, and it's mentioned in the welcome message which is automatically sent ot new subscribers. ======================================================================== Date: Tue, 2 Mar 93 14:00:38 CST From: "John Ladwig" Message-Id: <9303022000.AA06597@saturn> To: nih-image@soils.umn.edu Subject: Image mailing list archives available on gopher Several people have asked the following question: > Is it possible to get previous messages? Currently, I do not have email archive access to historical messages From the mailing list. I am investigating ways to do this, but they Received: from med.unc.edu (durham.med.unc.edu) by nx1.soils.umn.edu (5.65c) id AA06898; Thu, 29 Apr 1993 16:51:53 -0500 Return-Path: Received: from hasty.med.unc.edu by med.unc.edu (4.1/SMI-4.0-ACB-1.0) id AA10952; Thu, 29 Apr 93 17:54:51 EDT Received: by hasty.med.unc.edu (4.1/SMI-4.1) id AA06910; Thu, 29 Apr 93 17:54:49 EDT Date: Thu, 29 Apr 93 17:54:49 EDT From: refried@med.unc.edu (Howard M. Fried) Message-Id: <9304292154.AA06910@hasty.med.unc.edu> To: nih-image@soils.umn.edu Subject: Re: Straw Poll: Create comp.sys.mac.scitech? Read some Usenet groups Y Read sci.image.processing N Read comp.sys.mac.programmer N Read comp.sys.mac.applications Y Would read comp.sys.mac.scitech Y Would read sci.comp-aided if it had traffic Y Would vote on the comp.sys.mac.scitech CFV Y If so, how would you vote? Y From msachs@admin.ogi.edu Thu Apr 29 08:24:52 1993 Received: from ogicse.cse.ogi.edu (cse.ogi.edu) by nx1.soils.umn.edu (5.65c) id AA07118; Thu, 29 Apr 1993 17:23:40 -0500 Return-Path: Received: by ogicse.cse.ogi.edu (/\==/\ Smail3.1.25.1 #25.3) id ; Thu, 29 Apr 93 15:25 PDT Received: from [129.95.74.150] (atalk30.ese.ogi.edu) by admin.ogi.edu.ogi.edu (4.1/SMI-4.1) id AA18137; Thu, 29 Apr 93 15:24:54 PDT Date: Thu, 29 Apr 93 15:24:52 PDT Message-Id: <9304292224.AA18137@admin.ogi.edu.ogi.edu> To: From: msachs@admin.ogi.edu (Matthew Sachs) Subject: Re: Straw Poll: Create comp.sys.mac.scitech? >There is currently a discussion in news.groups regarding the possible >creation of a Usenet newsgroup comp.sys.mac.scitech. Generally, the >opponents of the group point out that there already exists a group >sci.comp-aided which has essentially no traffic, and should be used >instead. As I recall, the proposers will archive the group and include >an archive of software. The RFD has expired at my Usenet server, so >I can't include a copy. I will post a request that it be reposted. > >Interested readers of the NIH Image mailing list, please answer this >poll by entering Y or N at the end of each line: > >Read some Usenet groups y >Read sci.image.processing n| >Read comp.sys.mac.programmer n| >Read comp.sys.mac.applications n| >Would read comp.sys.mac.scitech n| _> >Would read sci.comp-aided if it had traffic ? I just started image reading this week >Would vote on the comp.sys.mac.scitech CFV don't know >If so, how would you vote? " Best, Matthew Sachs From MNOWYCKY@MEDCOLPA.BITNET Tue Apr 30 04:44:05 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA14352; Fri, 30 Apr 1993 17:18:10 -0500 Return-Path: Received: from MEDCOLPA.BITNET (MNOWYCKY@MEDCOLPA) by vx.cis.umn.edu (PMDF #2574 ) id <01GXMI7BGHN49AOJTL@vx.cis.umn.edu>; Fri, 30 Apr 1993 15:30:40 CDT Received: from ccc.medcolpa.edu by ccc.medcolpa.edu (PMDF #4083 ) id <01GXM92YNJE891VSLM@ccc.medcolpa.edu>; Fri, 30 Apr 1993 09:44:05 -0500 Date: 30 Apr 1993 09:44:05 -0500 From: MNOWYCKY@MEDCOLPA.BITNET Subject: Re: Zeiss Camera To: nih-image@soils.umn.edu Message-Id: <01GXM92YNT1E91VSLM@ccc.medcolpa.edu> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT unsubscribe From schmidth@vax.rz.uni-wuerzburg.dbp.de Sat May 1 21:09:27 1993 Received: from ixgate.gmd.de by nx1.soils.umn.edu (5.65c) id AA15783; Sat, 1 May 1993 12:10:18 -0500 Return-Path: X400-Received: by mta ixgate.gmd.de in /PRMD=GmD/ADMD=DbP/C=DE/; Relayed; Sat, 1 May 1993 19:08:58 +0200 X400-Received: by /PRMD=uni-wuerzburg/ADMD=dbp/C=de/; Relayed; Sat, 1 May 1993 19:09:27 +0200 Date: Sat, 1 May 1993 19:09:27 +0200 X400-Originator: schmidth@vax.rz.uni-wuerzburg.dbp.de X400-Recipients: nih-image@soils.umn.edu X400-Mts-Identifier: [/PRMD=uni-wuerzburg/ADMD=dbp/C=de/;930501190927] X400-Content-Type: P2-1984 (2) Content-Identifier: 18 From: Harald Schmidt Message-Id: <18*/S=schmidth/OU=vax/OU=rz/PRMD=uni-wuerzburg/ADMD=dbp/C=de/@MHS> To: nih-image In-Reply-To: <01GXM92YNT1E91VSLM@ccc.medcolpa.edu> Subject: Re: Zeiss Camera What does this message mean? Am I already been kicked out of the nih-image server? And what about Zeiss? Wondering. Harald. From set@eru.mt.luth.se Mon May 3 14:55:19 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA17659; Mon, 3 May 1993 05:54:03 -0500 Return-Path: Received: from [130.240.1.173] (osse216.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA00431; Mon, 3 May 1993 12:55:19 +0200 Date: Mon, 3 May 1993 12:55:19 +0200 Message-Id: <199305031055.AA00431@eru.mt.luth.se> To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: Re: Straw Poll: Create comp.sys.mac.scitech? X-Content-Type: Text/Quoted-Readable >There is currently a discussion in news.groups regarding the possible >creation of a Usenet newsgroup comp.sys.mac.scitech. Generally, the >opponents of the group point out that there already exists a group >sci.comp-aided which has essentially no traffic, and should be used >instead. As I recall, the proposers will archive the group and include >an archive of software. The RFD has expired at my Usenet server, so >I can't include a copy. I will post a request that it be reposted. > >Interested readers of the NIH Image mailing list, please answer this >poll by entering Y or N at the end of each line: > >Read some Usenet groups Y >Read sci.image.processing Y >Read comp.sys.mac.programmer Y >Read comp.sys.mac.applications N >Would read comp.sys.mac.scitech Y >Would read sci.comp-aided if it had traffic N >Would vote on the comp.sys.mac.scitech CFV N >If so, how would you vote? > >-- >Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 >Keck Laboratory for Biomolecular Imaging >NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From wayne@zippy.nimh.nih.gov Mon May 3 11:14:26 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA18448; Mon, 3 May 1993 11:14:51 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA26558; Mon, 3 May 93 12:25:40 -0400 Message-Id: <9305031625.AA26558@zippy.nimh.nih.gov> Date: Mon, 3 May 1993 11:14:26 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Zeiss Camera >unsubscribe To unsubscribe to the nih image mailing list send a message containing the single line "unsubscribe nih-image" to listserv@soils.umn.edu. --wayne From huff@MCCLB0.MED.NYU.EDU Mon May 3 09:37:10 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA00862; Mon, 3 May 1993 14:50:09 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXQQ7WGXKG0002OB@MCCLB0.MED.NYU.EDU>; Mon, 3 May 1993 14:37:17 EDT Date: Mon, 03 May 1993 14:37:10 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: CFV: comp.sys.mac.scitech To: nih-image@soils.umn.edu Message-Id: <01GXQQ7WQKSI0002OB@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT Thanks to all who responded to the straw poll. Discussion had sort of died down in news.groups, except for a few diehard opponents of the group, and I was going to post the responses to counter their arguments... However, in the meantime, the CFV has come out. Vote early and often :-) Path: netnews!cmcl2!yale.edu!ira.uka.de!howland.reston.ans.net!zaphod.mps.ohio-sta te.edu!cs.utexas.edu!uunet!bounce-back From: Michael Duncan Newsgroups: news.announce.newgroups,news.groups,sci.comp-aided,comp.sys.mac.advocacy,com p.sys.mac.apps,comp.sys.mac.announce Subject: CFV: comp.sys.mac.scitech Followup-To: poster Date: 30 Apr 1993 18:42:40 -0400 Organization: Naval Research Laboratory Lines: 116 Sender: tale@rodan.UU.NET Approved: tale@uunet.uu.net, werner@rascal.ics.utexas.edu Message-ID: <1rsa10INN564@rodan.UU.NET> NNTP-Posting-Host: rodan.uu.net Xref: netnews news.announce.newgroups:3779 news.groups:73049 sci.comp-aided:287 comp.sys.mac.advocacy:5589 comp.sys.mac.apps:22477 comp.sys.mac.announce:439 C A L L F O R V O T E S --------------------------- This is an official Call For Votes (CFV) for the creation of the new news group comp.sys.mac.scitech. STATUS unmoderated VOTING The voting period will begin when this CFV is posted. It will end on May 30, 1993 at 23:59 EDT. According to the USENET rules, in order for the group to be created, at least 2/3 of the votes cast must be YES votes, and there must be at least 100 more YES votes than NO votes. To vote please mail to the following address scitech@ra.nrl.navy.mil The contents of the message should contain either the line "I vote for comp.sys.mac.scitech as proposed" or "I vote against comp.sys.mac.scitech as proposed" whichever is appropriate. Email messages sent to the above address must constitute unambiguous and unconditional votes for/against newsgroup creation as proposed. Conditional votes will not be accepted. Votes emailed to the above address will be counted. In the event that more than one vote is placed by an individual, only the most recent vote will be counted. Please include your full name if your mailer doesn't do this for you. Two additional CFV's will be posted during the course of the vote, along with acknowledgments of those votes received to date. No information will be supplied as to how people are voting until the final acknowledgment is made at the end, at which time the full vote will be made public. SCHEDULE This CFV was issued on April 30, and the voting period will end on May 30, 1993 at 23:59 EDT. Thank you for your participation. CHARTER comp.sys.mac.scitech (unmoderated) will be a newsgroup for the discussion of practical and innovative applications of the Macintosh in a variety of scientific and engineering disciplines. Some, but not all, of the fields expected to be included within the scope of the newsgroup are: * Acoustics * Astronomy * Biochemistry * Biology * Chemistry * Computer Science * Electrical Engineering * Geology * Geophysics * Materials Science * Mathematics * Mechanical Engineering * Medicine * Optics * Physics * Psychology There are a number of technologies that are common to many of the above scientific fields. This newsgroup will also be for the discussion of advances in and applications of those technologies. Some, but not all, of the technologies of interest are: * Computer Aided Drawing/Computer Aided Engineering * Digital Signal Processing * Data Acquisition * Image Processing * Modeling * Process Control * Simulation * Technical Graphing * Technical Publishing * Visualization Since many scientists and engineers develop technical programs on the Macintosh computer, this newsgroup will also be for the discussion of topics in programming that relate to the use of programs in a science or engineering environment. It is assumed that this newsgroup will also include discussions of the Macintosh computer as it is used in the higher level education of scientists and engineers. RFD An RFD for this group was issued on March 31 and lasted for 30 days. -- Michael D. Duncan, Research Physicist scitech@ra.nrl.navy.mil Code 5640 Chairman, communications committee, MacSciTech Naval Research Laboratory Washington, DC 20375 (202) 767-2507 -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From huff@MCCLB0.MED.NYU.EDU Mon May 3 10:54:36 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA00916; Mon, 3 May 1993 15:01:06 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GXQSWX0BJ40002S5@MCCLB0.MED.NYU.EDU>; Mon, 3 May 1993 15:54:39 EDT Date: Mon, 03 May 1993 15:54:36 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Solicit reviews of image analysis apps To: nih-image@soils.umn.edu Message-Id: <01GXQSWXA8EA0002S5@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT Attached is my response to this posting. Anyone else with opinions should also reply to Dr. Wetzel. >Newsgroups: sci.image.processing >From: WTZL@db1.cc.rochester.edu (DR. DANIEL WETZEL) >Subject: Solicit reviews of image analysis apps >Date: Fri, 30 Apr 93 17:37:33 GMT >Lines: 19 > >I am interested in reviews of applications (public domain & "shrink wrapped") >for image analysis in the biological/medical sciences. In particular, apps >used for quantitative analysis of x-ray film and microsopic samples (i.e. >autoradiography and morphometery). Please supply info regarding >1) platform >2) operating system >3) supplier address >4) current list price >5) requirements: memory, framegrabbers, imaging cards/board sets etc. >6) device drivers supported (i.e. x-y-z stages; filter wheels) >7) your review: include positive & negatives, bugs, product support etc. >8) would you buy/download it again? >Please email reviews to: >WTZL@db1.cc.rochester.edu >I will summarize and post to this group if warranted. >Thanks >Dan Wetzel >UOR Neurology 1) platform Mac with 8 bit color and FPU. Version for nonFPU Macs available. 2) operating system System 6 or 7. 3) supplier address zippy.nimh.nih.gov, /pub/image, written by Wayne Rasband. Also available at many Mac FTP sites like sumex. 4) current list price $0.00, public domain, no strings, source available. 5) requirements: memory, framegrabbers, imaging cards/board sets etc. Might run in under 4 meg RAM, I haven't tried recently. Supports a number of Mac framegrabbers, and more are coming online since it the latest version supports Photoshop plug-ins. Requires Think Pascal in order to make source code modifications, $165 from MacWarehouse. 6) device drivers supported (i.e. x-y-z stages; filter wheels) Supports serial output to Mac modem port. 7) your review: include positive & negatives, bugs, product support etc. Does not support 16 bit images, but will import them and rescale them to match the current brightness / contrast. There is a mailing list of active users. Many people think the user interface is superior to commercial packages that they already own, and when possible, they use NIH Image in preference to those other packages. A number of users plan to add more features, even when these features are available in a commercial package, so that they do not have to teach more than one package. The macro programming language is Pascal like, crash free (unlike writing Pascal programs), and powerful. It supports both automation of things you can do From the menu, and programming things that cannot be done from the menu. Received: from pennsy.med.jhu.edu by nx1.soils.umn.edu (5.65c) id AA04314; Tue, 4 May 1993 08:57:37 -0500 Return-Path: Received: from [128.220.90.219] by pennsy.med.jhu.edu (5.65/1.34) id AA17515; Tue, 4 May 93 10:10:56 -0400 Date: Tue, 4 May 93 10:10:56 -0400 Message-Id: <9305041410.AA17515@pennsy.med.jhu.edu> To: nih-image@soils.umn.edu From: bsimon@pennsy.med.jhu.edu (Brett A. Simon) Subject: Data loss with 16 bit-Pict conversion? I am a new Image user and have a question about the different file types. I am importing signed 16 bit NEMA CT images, scaling them using the "SetImportMinMax" macro function, and making quantitative density measurements which need to be consistent from scan to scan. Saving my scaled images as Tiff or Pict files will greatly conserve disk space, but there is a veiled warning in the manual about changes in pixel values in pseudocolored images saved in Pict format, due to the data compression scheme. A quick comparison of random values and standard deviations of ROI's in some of my images saved in all three ways doesn't appear different, but this doesn't test all possibilities. Can anyone advise me if Pict format will actually alter density measurements in this situation? I have been using only the standard grayscale LUT, if that makes a difference. How about Tiff? Thanks for your help. B. Simon _____ Brett Simon (bsimon@pennsy.med.jhu.edu) Dept. of Anesthesia and Critical Care Medicine Johns Hopkins Hospital (410) 614-1515 From JCAIRA@UCONNVM.BITNET Mon May 6 07:30:34 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA21135; Thu, 6 May 1993 13:20:33 -0500 Return-Path: Received: from UCONNVM.UCONN.EDU (MAILER@UCONNVM) by vx.cis.umn.edu (PMDF #2574 ) id <01GXUQQKQR3K9AQMV9@vx.cis.umn.edu>; Thu, 6 May 1993 12:13:21 CDT Received: from UCONNVM (JCAIRA) by UCONNVM.UCONN.EDU (Mailer R2.08 R208004) with BSMTP id 0600; Thu, 06 May 93 12:35:03 EST Date: 06 May 1993 12:30:34 -0500 (EST) From: "P.R. Cislo" Subject: colorimage To: IMAGE Message-Id: <01GXUS2N4O0I9AQMV9@vx.cis.umn.edu> X-Envelope-To: NIH-IMAGE@soils.umn.edu Content-Transfer-Encoding: 7BIT I have a Quadra 950 with 4Mb of RAM. The Colorimage software package will not run. The software crashes right after the 'tool' icons for the package appear on the screen. Does anyone have a helpful suggestion? Thank you. P.R. Cislo University of Connecticut Marine Sciences Department Avery Point From jladwig@soils.umn.edu Thu May 6 10:52:44 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA22902; Thu, 6 May 1993 15:51:59 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA07476; Thu, 6 May 93 15:52:44 CDT Date: Thu, 6 May 93 15:52:44 CDT From: "John Ladwig" Message-Id: <9305062052.AA07476@saturn.soils.umn.edu> To: nih-image@soils.umn.edu Subject: Looping messages *should* be eliminated I'm terribly sorry about the out-of-control message looping problem we experienced earlier today. The cause was a mailer in Germany which does not recognize the Errors-to: mail header which is designed to eliminate this kind of problem. I have removed the user subscribed from the bad system and have infformed the postmaster at that location of the exact nature of the problem. If you notice that there are problems, I welcome *direct* mail either to my personal account, or to "owner-nih-image@soils.umn.edu" "postmaster@soils.umn.edu" is also good. I do receive a copy of each message sent to the list, and Ed Nater looks over them as well. I will generally know about problems as soon as I (or Ed) look at my (or his) mail, so sending additional message isn't specifically needed. In the future, *please* do not send more messages to the list when it can seen to be looping. This only fuels an already bad situation. It's a good thing I wasn't investigating our building network any longer than I was. The quoted message exacerbated the volume of looped/bounced messages to a size that it wouldn't have reached naturally for at least another hour or two. -jml NIH-Image mailing list administrator -- UMN Department of Soil Science; St. Paul, MN Internet: john.ladwig@soils.umn.edu Fidonet: John Ladwig 1:282/341 jladwig@torpedo.forestry.umn.edu Phone: +1-612-625-9235 From tyshih@cc.nctu.edu.tw Fri May 7 03:42:04 1993 Received: from nisc.jvnc.net by nx1.soils.umn.edu (5.65c) id AA25776; Fri, 7 May 1993 03:42:04 -0500 Return-Path: Received: from NCTU.edu.tw by nisc.jvnc.net (5.65/1.34) id AA02792; Fri, 7 May 93 04:43:52 -0400 Received: from ccserv.cc.nctu.edu.tw by NCTU.edu.tw with SMTP (16.8/16.2) id AA12654; Fri, 7 May 93 16:40:05 +0800 Received: from ccsun7.cc.nctu.edu.tw by cc.nctu.edu.tw (4.1/SMI-4.1) id AA12373; Fri, 7 May 93 16:43:48 CST Received: by ccsun7.cc.nctu.edu.tw (4.1/SMI-4.1) id AA02770; Fri, 7 May 93 16:43:47 CST From: tyshih@cc.nctu.edu.tw Message-Id: <9305070843.AA02770@ccsun7.cc.nctu.edu.tw> Subject: ImageFractal To: nih-image@soils.umn.edu Date: Fri, 7 May 93 16:43:46 WST Cc: tyshih@cc.nctu.edu.tw In-Reply-To: <9305041410.AA17515@pennsy.med.jhu.edu>; from "Brett A. Simon" at May 6, 93 7:53 am X-Mailer: ELM [version 2.3 PL11] Dear Netters: Greetings. I am trying to use ImageFractal. However, I can't get the fractal analysis going. Is there any document regarding to ImageFractal? Or, would anyone be kindly provide me some instructions? Thanks a lot for your kind attention. Sincerely yours Peter Shih =============================================================================== Peter T.Y. Shih Internet: tyshih@cc.nctu.edu.tw Surveying Engineering Group Dept. of Civil Engineering Phone: (886) 035-712121 exit 3540 National Chiao-Tung University FAX: (886) 035-716257 Hsin-Chu, Taiwan, R.O.C. Telex: ================================================================================ From wayne@zippy.nimh.nih.gov Fri May 7 09:18:11 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA27281; Fri, 7 May 1993 09:18:07 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03339; Fri, 7 May 93 10:29:31 -0400 Message-Id: <9305071429.AA03339@zippy.nimh.nih.gov> Date: Fri, 7 May 1993 09:18:11 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: v1.40b40 and RasterOps frame grabbers I have uploaded a new 1.50 beta to Zippy that should allow Image to capture images from any RasterOps frame grabber using the RasterOps Video Capture plug-in. --wayne From mcarter@iastate.edu Fri May 7 04:32:59 1993 Received: from iastate.edu (pv3229.vincent.iastate.edu) by nx1.soils.umn.edu (5.65c) id AA27474; Fri, 7 May 1993 09:31:11 -0500 Return-Path: Received: by iastate.edu with sendmail-5.57/4.7 id ; Fri, 7 May 93 09:33:00 -0500 From: mcarter@iastate.edu Message-Id: <9305071433.AA04067@iastate.edu> To: Subject: Re: v1.40b40 and RasterOps frame grabbers In-Reply-To: Your message of Fri, 07 May 93 09:24:24 -0500. <9305071429.AA03339@zippy.nimh.nih.gov> Date: Fri, 07 May 93 09:32:59 CDT Wayne, I'm in the process of setting up a system to use Image. Do you still recommend the Scion board as the frame grabber of choice, or would you now go with the RasterOps? Any advantage of one board over another? Thank-you, Margie Carter Image Analysis Facility Iowa State University mcarter@iastate.edu From rudolfo@hoh.mbl.edu Fri May 7 10:14:51 1993 Received: from AQUA.WHOI.EDU by nx1.soils.umn.edu (5.65c) id AA28022; Fri, 7 May 1993 10:14:51 -0500 Return-Path: Received: by aqua.whoi.edu (5.57/Ultrix3.0-C) id AA01875; Fri, 7 May 93 11:16:39 -0400 Received: from Mac-mailer (STARMAC13.MBL.EDU) by hoh.mbl.edu. (4.1/SMI-4.1) id AA18238; Fri, 7 May 93 11:12:31 EDT Message-Id: <9305071512.AA18238@hoh.mbl.edu.> Date: Fri, 07 May 93 11:22:14 From: rudolfo@hoh.mbl.edu (Rudolf Oldenbourg) To: Subject: DAC support on LG-3, "Make Movie" macro One piece of information and one question: Tod Weinberg from Scion Corp. has written code for NIH Image 1.49 which can set the two Digital to Analog Converters (DACs) on the Scion LG-3 board by using a menu command or macro functions. Tod (301- 695-7870) will send the special version of Image and/or source code to interested users. If requested, I can upload the files to zippy.nimh.nih.gov. My question, which might be best addressed to Wayne Rasband, has to do with the "Make Movie" menu command. It seems to me that there is no equivalent macro function providing the same speed and flexibility in grabbing several frames or frame portions and asigning them to a stack. Can this macro function be included in the next version of Image? Rudolf (another ardent Image user) From kartenh@Sdsc.Edu Fri May 7 16:07:56 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA28424; Fri, 7 May 1993 11:06:32 -0500 Return-Path: Date: Fri, 7 May 93 16:07:56 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930507160756.2485d05d@sds.sdsc.edu> Subject: RE: DAC support on LG-3, "Make Movie" macro To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Rudolf, How would you use the two DACs on the LG-3? I just purchased the board and am still exploring it uses. Unfortunately, the manual is "coming soon" but not yet available. Harvey Karten Harvey J. Karten, M.D. Dept. of Neurosciences Univ.California San Diego La Jolla, CA 92093-0608 EMail: Kartenh@sdsc.edu Phone: (619)-534-4938 FAX: (619)-534-6602 From STPREMW@production.unocal.com Fri May 7 02:11:00 1993 Received: from unogate.unocal.com by nx1.soils.umn.edu (5.65c) id AA28511; Fri, 7 May 1993 11:12:28 -0500 Return-Path: Received: from st.unocal.com (unocal-3.st.unocal.com) by unogate.unocal.com (4.1/SMI-4.1) id AA28709; Fri, 7 May 93 09:14:14 PDT Received: from courier by st.unocal.com (4.1/SMI-4.1) id AA26402; Fri, 7 May 93 09:14:09 PDT Received: by courier with Microsoft Mail id <2BEA8A22@courier>; Fri, 07 May 93 09:11:14 PDT From: "Withjack, Eric" To: nih-image@soils.umn.edu Subject: colorimage Date: Fri, 07 May 93 09:11:00 PDT Message-Id: <2BEA8A22@courier> Encoding: 8 TEXT X-Mailer: Microsoft Mail V3.0 FYI I have a Quadra 800 with 28 MB RAM and Image 1.49 runs fine. I noticed that the earlier version of Image(1.39b14) did not run well - especially changing the color scheme. This may be related to the new Mac OP SYS (7), as the old Image worked OK with Mac OP SYS 6. Eric Withjack, UNOCAL, Brea (714) 577-1227 From kartenh@Sdsc.Edu Fri May 7 17:46:46 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA29119; Fri, 7 May 1993 12:47:42 -0500 Return-Path: Date: Fri, 7 May 93 17:46:46 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930507174646.2485d05d@sds.sdsc.edu> Subject: RE: DAC support on LG-3, "Make Movie" macro To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Rudolf, Please upload the new LG-3 software. many thanks, Harvey Karten From rudolfo@hoh.mbl.edu Fri May 7 13:10:12 1993 Received: from AQUA.WHOI.EDU by nx1.soils.umn.edu (5.65c) id AA29289; Fri, 7 May 1993 13:10:12 -0500 Return-Path: Received: by aqua.whoi.edu (5.57/Ultrix3.0-C) id AA03400; Fri, 7 May 93 14:12:00 -0400 Received: from Mac-mailer (STARMAC13.MBL.EDU) by hoh.mbl.edu. (4.1/SMI-4.1) id AA19545; Fri, 7 May 93 14:07:52 EDT Message-Id: <9305071807.AA19545@hoh.mbl.edu.> Date: Fri, 07 May 93 14:17:35 From: rudolfo@hoh.mbl.edu (Rudolf Oldenbourg) To: Subject: Re: RE: DAC support on LG-3, "Make Movie" macro > How would you use the two DACs on the LG-3? I control liquid crystal retarders in a polarized light microscope. The electronic control circuits of the variable retarders (from CRI in Cambridge, MA) take an input voltage between 0 and 5V which is used to set the retardance of the LC devices. I use the DAC's to set the input voltages to computer controlled levels. > Please upload the new LG-3 software. WHat is the policy of uploading to zippy. Can anybody do it? Wayne or a system administrator, please respond. Rudolf From wayne@zippy.nimh.nih.gov Fri May 7 13:20:41 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA29401; Fri, 7 May 1993 13:20:42 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03923; Fri, 7 May 93 14:32:01 -0400 Message-Id: <9305071832.AA03923@zippy.nimh.nih.gov> Date: Fri, 7 May 1993 13:20:41 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: RE: DAC support on LG-3, "Make Movie" macro >WHat is the policy of uploading to zippy. Can anybody do it? Wayne or >a system administrator, please respond. Anyone can upload to the /pub/image/contrib directory on Zippy. --wayne From wayne@zippy.nimh.nih.gov Fri May 7 13:23:09 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA29445; Fri, 7 May 1993 13:23:05 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03932; Fri, 7 May 93 14:34:29 -0400 Message-Id: <9305071834.AA03932@zippy.nimh.nih.gov> Date: Fri, 7 May 1993 13:23:09 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: v1.40b40 and RasterOps frame grabbers > I'm in the process of setting up a system to use Image. Do you >still recommend the Scion board as the frame grabber of choice, or would >you now go with the RasterOps? Any advantage of one board over another? The advantage of the RasterOps 24STV is its ability to capture and display 24-bit color in real-time. It can do this because it is a frame grabber and video card rolled into one. The disadvantage is it that it is cumbersome to use a 24-bit card like the 24STV with an 8-bit program like Image. To capture an image you have to load the RasterOps plug-in, click on the OK button when it asks if you want to switch to millions of colors mode, wait for it to switch modes(several seconds), click on the Grab button, wait for the data to be copied to a 3 slice(RGB) stack, wait for Image to switch back to 8-bit mode, and, finally, wait for Image to generate a 8-bit version of the 24-bit image. With the 24STV you also give up the ability to average frames, to do shading correction, and to make movies. --wayne From rudolfo@hoh.mbl.edu Fri May 7 16:28:21 1993 Received: from AQUA.WHOI.EDU by nx1.soils.umn.edu (5.65c) id AA00689; Fri, 7 May 1993 16:28:21 -0500 Return-Path: Received: by aqua.whoi.edu (5.57/Ultrix3.0-C) id AA04813; Fri, 7 May 93 17:30:09 -0400 Received: from Mac-mailer (STARMAC13.MBL.EDU) by hoh.mbl.edu. (4.1/SMI-4.1) id AA23902; Fri, 7 May 93 17:26:01 EDT Message-Id: <9305072126.AA23902@hoh.mbl.edu.> Date: Fri, 07 May 93 17:35:45 From: rudolfo@hoh.mbl.edu (Rudolf Oldenbourg) To: Subject: Re: DAC support on LG-3 We have uploaded Image149LG3DAC.sit.hqx to the contrib directory on zippy.nimh.nih.gov. This archive contains the source and an executable version of Image 1.49 modified to support the D to A converters on the Scion LG-3 board. Please direct questions on its usage to Tod Weinberg, Scion Corp., Tel.: 301-695-7870. From wayne@zippy.nimh.nih.gov Fri May 7 16:32:05 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA00748; Fri, 7 May 1993 16:32:01 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA04379; Fri, 7 May 93 17:43:25 -0400 Message-Id: <9305072143.AA04379@zippy.nimh.nih.gov> Date: Fri, 7 May 1993 16:32:05 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Data loss with 16 bit-Pict conversion? >I am a new Image user and have a question about the different file types. >I am importing signed 16 bit NEMA CT images, scaling them using the >"SetImportMinMax" macro function, and making quantitative density >measurements which need to be consistent from scan to scan. Saving my >scaled images as Tiff or Pict files will greatly conserve disk space, but >there is a veiled warning in the manual about changes in pixel values in >pseudocolored images saved in Pict format, due to the data compression >scheme. The pixels values do not change due to the compression, but they sometimes change because QuickDraw's DrawPicture routine is more concerned with getting the screen colors right than with maintaining the pixel values. >A quick comparison of random values and standard deviations of >ROI's in some of my images saved in all three ways doesn't appear >different, but this doesn't test all possibilities. > Can anyone advise me if Pict format will actually alter density >measurements in this situation? I have been using only the standard >grayscale LUT, if that makes a difference. How about Tiff? I have only seen this problem with pseudocolored images saved in PICT format. Pixels values are never altered when saving in TIFF format. --wayne From stmwang@hubcap.clemson.edu Fri May 7 17:29:25 1993 Received: from hubcap.clemson.edu by nx1.soils.umn.edu (5.65c) id AA01959; Fri, 7 May 1993 20:28:07 -0500 Return-Path: Received: by hubcap.clemson.edu; Fri, 7 May 93 21:29:26 -0400 From: stmwang@hubcap.clemson.edu (Sam Wang) Message-Id: <9305080129.AA15792@hubcap.clemson.edu> Subject: Frame averaging To: nih-image@soils.umn.edu Date: Fri, 7 May 1993 21:29:25 -0400 (EDT) In-Reply-To: <9305071834.AA03932@zippy.nimh.nih.gov> from "Wayne Rasband" at May 7, 93 01:23:33 pm X-Mailer: ELM [version 2.4 PL2] Content-Type: text Content-Length: 307 Wayne, Is there some way to average captured frames? For instance, can frames captured previously be averaged? If not currently possible, what about in the future? Thanks. Sam -- Sam Wang stmwang@hubcap.clemson.edu 803/656-3924voice 656-0204fax Art Dept., Clemson University, Clemson, SC 29634-0509 From ptr@greco2.polytechnique.fr Sat May 8 17:09:49 1993 Received: from chenas.inria.fr by nx1.soils.umn.edu (5.65c) id AA04594; Sat, 8 May 1993 08:09:01 -0500 Return-Path: Received: from polytechnique.polytechnique.fr by chenas.inria.fr (5.65c8d/92.02.29) via Fnet-EUnet id AA12798; Sat, 8 May 1993 15:10:45 +0200 (MET) Received: by polytechnique.polytechnique.fr (5.65c/SMI-4.1.3) id AA25610; Sat, 8 May 1993 15:16:48 +0200 Received: by poly.polytechnique.fr (5.57/DEC-Ultrix/4.3) id AA24473; Sat, 8 May 93 15:10:33 +0200 Received: from [129.104.27.245] by greco2.noname (4.1/SMI-4.1) id AA20224; Sat, 8 May 93 15:09:50 +0200 Date: Sat, 8 May 93 15:09:49 +0200 Message-Id: <9305081309.AA20224@greco2.noname> To: From: ptr@greco2.polytechnique.fr (Peter Goedtkindt) Subject: question for video hackers >The advantage of the RasterOps 24STV is its ability to capture and display >24-bit color in real-time. It can do this because it is a frame grabber and >video card rolled into one. The disadvantage is it that it is cumbersome to >use a 24-bit card like the 24STV with an 8-bit program like Image. To >capture an image you have to load the RasterOps plug-in, click on the OK >button when it asks if you want to switch to millions of colors mode, wait >for it to switch modes(several seconds), click on the Grab button, wait for >the data to be copied to a 3 slice(RGB) stack, wait for Image to switch >back to 8-bit mode, and, finally, wait for Image to generate a 8-bit >version of the 24-bit image. With the 24STV you also give up the ability to >average frames, to do shading correction, and to make movies. > >--wayne Does the RaterOps card has an external trigger? Are the 3 colors captured taken from the same frame or does it takes 3 frames to do a sequential grabbing. I'm still looking to grab images coming from 3 syncronized b/w CCD camera's.The 3 signals can electronically be encoded as a single composite color 'image', but I need to grab one precise image (defined by a trigger pulse) from all three camera's and I don't like to buy two additional quickcapture boards. Anybody has an idea how to build myself a frame store, which will freeze a b/w image when a trigger signal is applied? If such devices exist, I could add even more signals, and read them in sequentially into my quickcapture. Peter Goedtkindt Universite Paris-Sud - Laboratoire de Spectroscopie Atomique et Ionique bat350 - Centre D'Orsay F-91405 Orsay Cedex. France ; Fax.:++33.1.69.41.94.60 ----------------------------------------------------------------- Ecole Polytechnique, Laboratoire d'Utilisation de Lasers Intenses ----------------------------------------------------------------- From kartenh@Sdsc.Edu Sat May 8 16:58:10 1993 Received: from Sdsc.Edu (m5.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA05510; Sat, 8 May 1993 11:56:30 -0500 Return-Path: Date: Sat, 8 May 93 16:58:10 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930508165810.20405530@m5.sdsc.edu> Subject: Ludl 1000 Controller To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" Has anyone written any Macros for controlling the MAC 1000 Ludl controller for stage (x,y) and focus (z) movements? David Salzman was kind enough to provide assistance on getting started, but I am still having troubles with this. I have made some progress in penetrating the obtuse manual originally provided by Ludl. It is very brief, with many functions not explained. I have also been able to finally get control of the MAC 1000 via terminal emulator programs (I use Red Ryder 10.3 with good results). If there are other users out there who would like to exchange info on this, please let me know. I now can control about 12 out of the 17 functions built in to the MDRMS Controller. I need a bit of help with the other functions. The difficulty mainly resides in 1) the control codes; and 2) How Ludl intended the use of the "Base" and "Target" values. Looking forward to hearing from others needing liberation from Ludl. Harvey Karten Harvey J. Karten, M.D. Dept. Neurosciences UCSD 0608 La Jolla, CA 92093 Phone (619)-534-4938 FAX (619)-534-6602 E-Mail KARTENH@SDSC.EDU From JWALKER@SBBIOVM.BITNET Thu May 9 11:50:44 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA11227; Sun, 9 May 1993 14:49:25 -0500 Return-Path: Received: from SBBIOVM.BITNET (MAILER@SBBIOVM) by vx.cis.umn.edu (PMDF #2574 ) id <01GXZ4GRZVGG9FM7UA@vx.cis.umn.edu>; Sun, 9 May 1993 14:51:30 CDT Received: from SBBIOVM (JWALKER) by SBBIOVM.BITNET (Mailer R2.07) with BSMTP id 6941; Sun, 09 May 93 15:51:08 EDT Date: 09 May 1993 15:50:44 -0400 (EDT) From: jeff Walker Subject: unsub To: nih-image@soils.umn.edu Message-Id: <01GXZ4GRZVGI9FM7UA@vx.cis.umn.edu> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT please unsubsribe jeff Walker from the list nih-soils From set@eru.mt.luth.se Mon May 10 15:01:13 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA14786; Mon, 10 May 1993 05:59:32 -0500 Return-Path: Received: from [130.240.1.173] (osse216.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA18280; Mon, 10 May 1993 13:01:13 +0200 Date: Mon, 10 May 1993 13:01:13 +0200 Message-Id: <199305101101.AA18280@eru.mt.luth.se> To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: 1.50 Expansions X-Content-Type: Text/Quoted-Readable Some ideas: - Include PAL/NTSC in Videocontrol since it's tested and verified now. An urgent wish from us outside the states and a policy item for Apple. - In Image 1.49 LG3DAC-version please include the macro command DacControl( DacA, DacB : 0..255 ) instead/and SetDacA, SetDacB. It would make the DAC option a lot powerfull. Compareable to Serial-macros. - Is it possible to use the on-board memory on LG3 now for fast framegrabbing and to transfere the images to Image-windows by macros. Including commands/macro for delay between frames. - We are using the AFM version of Image with FFT, Wane did ask for the programdeveloper and it's probably U Knipping at Arizona State University, since they do have the 1.40 version. - We do also like to have FFT and 2Dxcorr functions in Image. Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From wayne@zippy.nimh.nih.gov Mon May 10 09:48:21 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA16904; Mon, 10 May 1993 09:48:24 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06736; Mon, 10 May 93 10:59:48 -0400 Message-Id: <9305101459.AA06736@zippy.nimh.nih.gov> Date: Mon, 10 May 1993 09:48:21 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: 1.50 Expansions > - Include PAL/NTSC in Videocontrol since it's tested and verified now. > An urgent wish from us outside the states and a policy item for Apple. I plan to include Sven-Erik Tiberg's method for more accurate Make Movie timing when using PAL systems in V1.50. > - In Image 1.49 LG3DAC-version please include the macro command >DacControl( DacA, DacB : 0..255 ) instead/and SetDacA, SetDacB. It would >make the DAC option a lot powerfull. Compareable to Serial-macros. I have added the SetDacA and SetDacB macros to 1.50b41. > - Is it possible to use the on-board memory on LG3 now for fast >framegrabbing and to transfere the images to Image-windows by macros. >Including commands/macro for delay between frames. There is currently no way to do this without custom Pascal programming. > - We do also like to have FFT and 2Dxcorr functions in Image. I have no plans to add FFT or 2D cross-correlation capabilities to Image. --wayne From set@eru.mt.luth.se Mon May 10 19:36:04 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA17342; Mon, 10 May 1993 10:34:22 -0500 Return-Path: Received: from [130.240.1.173] (osse216.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA27103; Mon, 10 May 1993 17:36:04 +0200 Date: Mon, 10 May 1993 17:36:04 +0200 Message-Id: <199305101536.AA27103@eru.mt.luth.se> To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: Re: 1.50 Expansions X-Content-Type: Text/Quoted-Readable >> - Is it possible to use the on-board memory on LG3 now for fast >>framegrabbing and to transfere the images to Image-windows by macros. >>Including commands/macro for delay between frames. > >There is currently no way to do this without custom Pascal programming. > >--wayne Thank you wayne for the modifications, we will now try to programm the LG-3 for this purouse. -- Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From MNOWYCKY@MEDCOLPA.BITNET Fri May 10 08:27:37 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA18066; Mon, 10 May 1993 12:26:46 -0500 Return-Path: Received: from MEDCOLPA.BITNET (MNOWYCKY@MEDCOLPA) by vx.cis.umn.edu (PMDF #2574 ) id <01GY0DR6KGSG9GV5MM@vx.cis.umn.edu>; Mon, 10 May 1993 12:28:46 CDT Received: from ccc.medcolpa.edu by ccc.medcolpa.edu (PMDF #4083 ) id <01GY0FSTIJMO94DQ9K@ccc.medcolpa.edu>; Mon, 10 May 1993 13:27:37 -0500 Date: 10 May 1993 13:27:37 -0500 From: MNOWYCKY@MEDCOLPA.BITNET Subject: Re: unsub To: nih-image@soils.umn.edu Message-Id: <01GY0FSTIT9U94DQ9K@ccc.medcolpa.edu> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT please unsubscribe martha nowycky from the list nih-soils From wayne@zippy.nimh.nih.gov Mon May 10 12:36:29 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA18203; Mon, 10 May 1993 12:36:33 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06978; Mon, 10 May 93 13:47:56 -0400 Message-Id: <9305101747.AA06978@zippy.nimh.nih.gov> Date: Mon, 10 May 1993 12:36:29 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Frame averaging >Is there some way to average captured frames? For instance, can frames >captured previously be averaged? If not currently possible, what about >in the future? Previously captured frames can be averaged using the new Average command(Stacks menu) in Image 1.49. V1.49 is available by anonymous FTP From zippy.nimh.nih.gov. Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA18261; Mon, 10 May 1993 12:39:22 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06982; Mon, 10 May 93 13:50:46 -0400 Message-Id: <9305101750.AA06982@zippy.nimh.nih.gov> Date: Mon, 10 May 1993 12:39:19 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: unsub >please unsubscribe martha nowycky from the list nih-soils To unsubscribe, send a one line message containing the line "unsubscribe nih-image" to listserv@soils.umn.edu. --wayne From @TECHNION.TECHNION.AC.IL:RPRDANI@TECHNION Sat May 11 20:12:21 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA25248; Tue, 11 May 1993 06:58:30 -0500 Return-Path: <@TECHNION.TECHNION.AC.IL:RPRDANI@TECHNION> Received: from TECHNION.TECHNION.AC.IL (MAILER@TECHNION) by vx.cis.umn.edu (PMDF #2574 ) id <01GY1GLIT3TC9GVBFA@vx.cis.umn.edu>; Tue, 11 May 1993 07:00:30 CDT Received: from TECHNION (NJE origin RPRDANI@TECHNION) by TECHNION.TECHNION.AC.IL (LMail V1.1c/1.7e) with BSMTP id 7255; Tue, 11 May 1993 14:59:57 +0200 Date: 11 May 1993 14:42:21 +0530 (IST) From: Danni Dagan Subject: CID cameras To: Image BB Message-Id: <01GY1GLIT3TE9GVBFA@vx.cis.umn.edu> X-Envelope-To: NIH-IMAGE@SOILS.UMN.EDU Content-Transfer-Encoding: 7BIT Does anybody have experience with ChargeInjectionDevice (CID) cameras? What would be a suitable Image Grabber board to match the speed of CIDs? The CIDTEC (CID camera manufacturere for civilian uses) claim a 1kHz rate (1000 fps) at 64x64 pixels. With 2x4 pixels one should be able to reach 0.5MHz rates. Information on CID cameras, Image Grabbers, and software to capture and load frames into Image windows will be greatly appreciated. Danni Dagan Biophysics & Physiology Rappaport Faculty of Medicine Sir Bernard Katz Minerva Center for Cell Biophysics +++++++++++++++++++++++++++++++++++++++++++++++++ Does anybody have experience with ChargeInjectionDevice (CID) cameras? What would be a suitable Image Grabber board to match the speed of CIDs? The CIDTEC (CID camera manufacturers for civilian uses) claim a 1kHz rate (1000 fps) at 64x64 pixels. With 2x4 pixels one should be able to reach 0.5MHz rates. Information on CID cameras, Image Grabbers, and software to capture and load frames into Image windows will be greatly appreciated. Danni Dagan Biophysics & Physiology Rappaport Faculty of Medicine Sir Bernard Katz Minerva Center for Cell Biophysics ======================================================================== 9 Date: Tue, 11 May 93 14:26:55 IST From: danni Subject: Re: CID cameras To: John Ladwig In-Reply-To: Your message of Tue, 11 May 93 06:21:41 CDT Thanks. My address should read RPRDANI@TECHNION.BITNET and not RPRD@.... no wonder it bounced. dd From ptr@GRECO2.POLYTECHNIQUE.FR Wed May 12 14:38:42 1993 Received: from chenas.inria.fr by nx1.soils.umn.edu (5.65c) id AA01261; Wed, 12 May 1993 05:38:01 -0500 Return-Path: Received: from polytechnique.polytechnique.fr by chenas.inria.fr (5.65c8d/92.02.29) via Fnet-EUnet id AA18461; Wed, 12 May 1993 12:39:38 +0200 (MET) Received: by polytechnique.polytechnique.fr (5.65c/SMI-4.1.3) id AA20475; Wed, 12 May 1993 12:45:43 +0200 Received: from [129.104.27.245] by greco2.noname (4.1/SMI-4.1) id AA20880; Wed, 12 May 93 12:38:42 +0200 Date: Wed, 12 May 93 12:38:42 +0200 Message-Id: <9305121038.AA20880@greco2.noname> To: From: ptr@GRECO2.POLYTECHNIQUE.FR (Peter Goedtkindt) Subject: HDTV HDTV is will be the new standard for high resolution imaging. The number of pixels on such a ccd are usually much more than 1000*1000, and the image is in the format 9/16 instead of 3/4. I do not think that the usual framegrabbers accept this standard. It is too new and the amount of data to be processed is quite large. Anyway, If somebody has experience with such camera's and related equipment, it would be nice to share it with this list. -PTR From norm_hurst@maca.sarnoff.com Wed May 12 08:58:37 1993 Received: from nova (nova.sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA02020; Wed, 12 May 1993 08:58:37 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA00596; Wed, 12 May 93 10:00:05 EDT Message-Id: <9305121400.AA00596@nova> Date: 12 May 1993 09:24:01 U From: "Norm Hurst" Subject: Re: HDTV To: nih-image@soils.umn.edu RE>HDTV The ONLY thing standard about HDTV is the aspect ratio: 16x9. There are four proposed scanning formats: "1050 interlaced", or twice CCIR-601: 1440x960, 59.94 fields/sec "787.5 proscan", or 3/2 525 lines with square pixels: 1280x720 59.94 frames/sec "1250 Interlaced" or twice 625, at 50 fields/sec: "PAL HDTV" "1125 Interlaced 60 fields/sec". This is the original Sony/NHK system, and the only format for which you can buy cameras and tape machines. All other formats are custom (for the moment). There is a religious war going on that focusses on three points: Square pixels Interlace or Progressive scan Frame rate (50, 60, 59.94 24/48/72, 25/50/75) The four HDTV proponents before the FCC are currently trying to avoid another round of technical tests ($20000/day for each proponent!) by forging a "Grand Alliance", a single, compromise system. The current deadline for this (i.e. testing begins) is May 24. Stay tuned! Norm Hurst David Sarnoff Research Center From losher@tigger.jvnc.net Wed May 12 12:42:40 1993 Received: from tigger.jvnc.net by nx1.soils.umn.edu (5.65c) id AA03714; Wed, 12 May 1993 15:41:06 -0500 Return-Path: Received: from [161.58.5.2] (mozart-tty2.jvnc.net) by tigger.jvnc.net with SMTP id AA26642 (5.65c/IDA-1.4.4 for nih-image@soils.umn.edu); Wed, 12 May 1993 16:42:40 -0400 Date: Wed, 12 May 1993 16:42:40 -0400 Message-Id: <199305122042.AA26642@tigger.jvnc.net> To: nih-image@soils.umn.edu From: losher@direct.com Subject: NormHurst re HDTV Cc: Multiple recipients of list Keep up the great HDTV info, Norm, it's greatly appreciated!! From losher@tigger.jvnc.net Wed May 12 12:42:49 1993 Received: from tigger.jvnc.net by nx1.soils.umn.edu (5.65c) id AA03744; Wed, 12 May 1993 15:41:20 -0500 Return-Path: Received: from [161.58.5.2] (mozart-tty2.jvnc.net) by tigger.jvnc.net with SMTP id AA26646 (5.65c/IDA-1.4.4 for nih-image@nx1.soils.umn.edu); Wed, 12 May 1993 16:42:49 -0400 Date: Wed, 12 May 1993 16:42:49 -0400 Message-Id: <199305122042.AA26646@tigger.jvnc.net> To: nih-image@soils.umn.edu From: losher@direct.com Subject: NormHurst re HDTV Cc: Multiple recipients of list Keep up the great HDTV info, Norm, it's greatly appreciated!! Morton Losher 25 Fox Hill Road Woodbridge, Conn. 06525-1427 e-mail(INTERNET): losher@direct.com e-mail(CompuServe): 73140,2007 telephone(FAX): 203-389-5235 From este@cs.ait.ac.th Fri May 14 21:40:24 1993 Received: from munnari.OZ.AU by nx1.soils.umn.edu (5.65c) id AA11270; Fri, 14 May 1993 08:37:29 -0500 Return-Path: Received: from cs4.cs.ait.ac.th by munnari.oz.au with SMTP (5.83--+1.3.1+0.50) id AA23936; Fri, 14 May 1993 23:32:27 +1000 (from este@cs.ait.ac.th) Message-Id: <9305141332.23936@munnari.oz.au> Received: by cs4.cs.ait.ac.th (4.1) id AA02455; Fri, 14 May 93 20:30:24+070 Date: Fri, 14 May 93 20:30:24+070 From: este@cs.ait.ac.th ( ESTE Armstrong) To: nih-image@soils.umn.edu Subject: subscribe subscribe From jamiel@sybase.com Fri May 14 14:28:43 1993 Received: from halon.sybase.com by nx1.soils.umn.edu (5.65c) id AA12979; Fri, 14 May 1993 14:28:43 -0500 Return-Path: Received: from sybase.com ([130.214.220.10]) by halon.sybase.com (5.0/SMI-SVR4/SybFW4.0) id AA14282; Fri, 14 May 93 11:43:43 PDT Received: from ralph.sybgate.sybase.com by sybase.com (4.1/SMI-4.1/SybH3.2) id AA16764; Fri, 14 May 93 11:41:43 PDT Received: from Mac-mailer ([130.214.134.8]) by ralph.sybgate.sybase.com (4.1/SMI-4.1/SybEC3.1) id AA23196; Fri, 14 May 93 11:41:41 PDT Message-Id: <9305141841.AA23196@ralph.sybgate.sybase.com> Date: Fri, 14 May 93 11:42:40 From: jamiel@sybase.com (Jamie Lawrence) To: nih-image@soils.umn.edu Subject: Image for non-research users? Content-Length: 0 Hi- I am an avid Image fan, and was wondering if there is any one else out there that use it as I do- for art rather than analysis. If so, then please resond. I am interested in what you do, technique talk, macros and convolution filters, mainly, but am also just curious if I am alone on this one. Thanks, Jamie From GSW$EN@WSUVM1.CSC.WSU.EDU Fri May 14 08:44:47 1993 Received: from wsuvm1.csc.wsu.edu by nx1.soils.umn.edu (5.65c) id AA13917; Fri, 14 May 1993 18:50:33 -0500 Return-Path: Message-Id: <199305142350.AA13917@nx1.soils.umn.edu> Received: from WSUVM1.CSC.WSU.EDU by WSUVM1.CSC.WSU.EDU (IBM VM SMTP V2R2) with BSMTP id 9409; Fri, 14 May 93 16:51:54 PST Received: from WSUVM1 (GSW$EN) by WSUVM1.CSC.WSU.EDU (Mailer R2.10 ptf000) with BSMTP id 5036; Fri, 14 May 93 16:51:54 PST Date: Fri, 14 May 93 16:44:47 PST From: Jerry Tangren Organization: WSU Tree Fruit Research & Extension Center Subject: Re: Image for non-research users? To: nih-image@soils.umn.edu In-Reply-To: Message of Fri, 14 May 1993 14:32:24 -0500 from jamie, this may not be what you are suggesting, but we use Image to color weather satellite photos for posting to a bulletin board accessed by tree fruit growers. We download the infrared photos, determine the minimum level for the clouds and then color the visible water blue and the land brown. Makes a nice gimmic for our computer bulletin board. Jerry Tangren Wash State Univ Tree Fruit Research & Extension Center Wenatchee WA From stmwang@hubcap.clemson.edu Fri May 14 16:07:08 1993 Received: from hubcap.clemson.edu by nx1.soils.umn.edu (5.65c) id AA14017; Fri, 14 May 1993 19:05:16 -0500 Return-Path: Received: by hubcap.clemson.edu; Fri, 14 May 93 20:07:09 -0400 From: stmwang@hubcap.clemson.edu (Sam Wang) Message-Id: <9305150007.AA09443@hubcap.clemson.edu> Subject: Re: Image for non-research users? To: nih-image@soils.umn.edu Date: Fri, 14 May 1993 20:07:08 -0400 (EDT) In-Reply-To: <9305141841.AA23196@ralph.sybgate.sybase.com> from "Jamie Lawrence" at May 14, 93 02:28:54 pm X-Mailer: ELM [version 2.4 PL2] Content-Type: text Content-Length: 574 Jamie, There are at least two of us! I am using Image for artistic explorations only. I also use it in my Art with Computer class. Image offers a lot in terms of image manipulations that's different from that of PhotoShop, and I find it refreshing. Have spend hours and hours playing with surface plot functions, showing fellow architects and sculptors how one can do a quick greyscale mockup and immediately turn it into 3D. Lots of fun. Sam -- Sam Wang stmwang@hubcap.clemson.edu 803/656-3924voice 656-0204fax Art Dept., Clemson University, Clemson, SC 29634-0509 From seaa@ecuanex.apc.org Sat May 15 14:45:03 1993 Received: from cdp.igc.org by nx1.soils.umn.edu (5.65c) id AA19151; Sun, 16 May 1993 04:50:47 -0500 Return-Path: Received: by igc.apc.org (4.1/Revision: 1.85 ) id AA00733; Sun, 16 May 93 02:52:05 PDT Received: by ecuanex.ec (5.61/1.35) id AA06826; Sat, 15 May 93 19:45:04 -0400 From: seaa@ecuanex.ec (Sociedad Ecuatoriana de Astronomia y Astrofisica) Message-Id: <9305152345.AA06826@ecuanex.ec> Subject: Re: Image for non-research users? To: nih-image@soils.umn.edu Date: Sat, 15 May 93 19:45:03 EST In-Reply-To: <9305141841.AA23196@ralph.sybgate.sybase.com>; from "Jamie Lawrence" at May 14, 93 2:29 pm X-Mailer: ELM [version 2.3 PL0 (Sp)] Jamie, although this is actually "research" it might give you some ideas. We are using Image to obtain digital files of astronomical photographic plates of the Omega Centauri globular cluster - one million stars in an approximately spherical cluster. Image is also used to measure the light output of the variable stars in the cluster, after the digital files are on disk. False color pictures of the star field created by Image are very dramatic. Regards, felipe fried From este@cs.ait.ac.th Mon May 17 10:21:22 1993 Received: from munnari.OZ.AU by nx1.soils.umn.edu (5.65c) id AA21805; Sun, 16 May 1993 21:12:07 -0500 Return-Path: Received: from cs4.cs.ait.ac.th by munnari.oz.au with SMTP (5.83--+1.3.1+0.50) id AA29975; Mon, 17 May 1993 12:13:20 +1000 (from este@cs.ait.ac.th) Message-Id: <9305170213.29975@munnari.oz.au> Received: by cs4.cs.ait.ac.th (4.1) id AA09680; Mon, 17 May 93 09:11:22+070 Date: Mon, 17 May 93 09:11:22+070 From: este@cs.ait.ac.th ( ESTE Armstrong) To: NIH-Image@soils.umn.edu Subject: statistical tests on profiles I am looking for ways to analyze and cluster profiles collected through Image. Currently I am using the Mahalanobis Distance to study the variability of my profiles and Sammon's non linear algorithm to help cluster the profiles. I would appreciate learning what other techniques people have used to analyze the multi-dimensional profiles. Este Armstrong Este@cs.ait.ac.th From CHARLEST@macc.wisc.edu Mon May 17 04:18:00 1993 Received: from vms2.macc.wisc.edu by nx1.soils.umn.edu (5.65c) id AA24111; Mon, 17 May 1993 10:17:36 -0500 Return-Path: Received: from VMSmail by vms2.macc.wisc.edu; Mon, 17 May 93 10:18 CST Message-Id: <23051710181129@vms2.macc.wisc.edu> Date: Mon, 17 May 93 10:18 CST From: Charles Thomas Subject: Re: Image for non-research users? To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu",CHARLEST Well, not to get off the scientific talk, but I too have dabbled with Image in an artistic fashion. I have some very nice pictures of castles I did by conceiving them in grey levels, then doing a surface plot and editing the surface plot pixel by pixel. All of this was, of course, done on my own time. Cheers! From herro001@staff.tc.umn.edu Mon May 17 06:31:58 1993 Received: from staff.tc.umn.edu by nx1.soils.umn.edu (5.65c) id AA24624; Mon, 17 May 1993 11:34:56 -0500 Return-Path: Received: by staff.tc.umn.edu; Mon, 17 May 93 11:36:30 -0500 Date: Mon, 17 May 1993 11:31:58 -0500 (CDT) From: "Michael J Herron-1" Subject: Re: Image for non-research users? To: nih-image@soils.umn.edu In-Reply-To: <23051710181129@vms2.macc.wisc.edu> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII It is nice to hear from the "art side" of Image users. I have "played" a lot with look up tables to pseudo color images from confocal pictures (which have no "real color" information). A big part of presenting data is selling the audience. I have found that people pay a lot more attention when your images are expected...or even unexpected colors. From SMIT@CABO.AGRO.NL Sat May 18 13:27:00 1993 Received: from hearnvax.nic.surfnet.nl by nx1.soils.umn.edu (5.65c) id AA28645; Tue, 18 May 1993 05:27:09 -0500 Return-Path: Received: from AGRO.NL (AGRO02) by HEARNVAX.nic.SURFnet.nl (PMDF #3330 ) id <01GYBK56NAKW00L3KF@HEARNVAX.nic.SURFnet.nl>; Tue, 18 May 1993 12:29:49 N Received: from CABO.AGRO.NL by AGRO.NL with PMDF#10389; Tue, 18 May 1993 12:28 MET Date: 18 May 1993 12:27 +0100 (MET) From: "A.L. SMIT" Subject: automatic fileprocessing To: nih-image@soils.umn.edu Message-Id: <34FFAD549F7FA00540@CABO.AGRO.NL> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: Q_IMAGE X-Vms-Cc: SMIT Content-Transfer-Encoding: 7BIT Hello, I would like to use Image to process several files during the night. I understood that this is possible and wonder if one of you could give me an example how it can be done. I would like to do the following things: -background correction (e.g. 2d ball) -sharpening -thresholding ( is it possible to do this automatically, e.g. with Isodata threshold method?) -skeletonizing the binary picture -counting the number of pixels in the skeletons (would it also be possible to discard skeletons smaller than e.g. 5 pixels?. (I am not asking to program this all for me (you may do it of course), but more an example how it can be done). Thanks in advance. Bert Smit, the Netherlands From wayne@zippy.nimh.nih.gov Wed May 19 12:15:30 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA06938; Wed, 19 May 1993 12:15:48 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA19807; Wed, 19 May 93 13:27:12 -0400 Message-Id: <9305191727.AA19807@zippy.nimh.nih.gov> Date: Wed, 19 May 1993 12:15:30 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: V1.50b51 beta and Filter plug-ins I have uploaded a new Image beta(1.50b51) to Zippy that: *Supports Photoshop filter plug-ins *Uses submenus for plug-ins *Fixes a bug that caused the Microtek Scanmaker plug-in to fail *Imports 3D data sets *Supports the Scion LG-3's external trigger, D/A converters and digital I/O ports Plug-ins must now be stored in a folder named "Plug-ins", located either in the same folder as Image or in the System Folder. 1.50b51 comes with example acquisition and filter plug-ins. Please let me know what acquisition and filter plug-ins seem to work so I can add them to the list in the Change History file. Are there any Think C experts out there? It would be nice if someone could figure out how to compile the example plug-ins in the plug-ins/developers_kit file on Zippy. They seem to expect a version of Think C earlier than 4.0. --wayne From Carl_Gustafson@cbis.ece.drexel.edu Wed May 19 12:30:33 1993 Received: from cbis.ece.drexel.edu by nx1.soils.umn.edu (5.65c) id AA07133; Wed, 19 May 1993 12:30:33 -0500 Return-Path: Received: from homer.ece.drexel.edu by cbis.ECE.Drexel.EDU (5.67/1.34) id AA03797; Wed, 19 May 93 13:32:00 EDT Message-Id: <9305191732.AA03797@cbis.ECE.Drexel.EDU> Date: 19 May 1993 13:27:42 U From: "Carl Gustafson" Subject: Re: V1.50b51 beta and Filter To: nih-image@soils.umn.edu Reply to: RE>V1.50b51 beta and Filter pl >Are there any Think C experts out there? It would be nice if someone could >figure out how to compile the example plug-ins in the >plug-ins/developers_kit file on Zippy. They seem to expect a version of >Think C earlier than 4.0. I'm not an expert, but off the top of my head I know that TC 5 and later is moving towards a header organization similar to MPW's. The problem is thus probably in the #includes in the source files. Regards, Carl Gustafson From jamiel@sybase.com Wed May 19 12:44:13 1993 Received: from halon.sybase.com by nx1.soils.umn.edu (5.65c) id AA07321; Wed, 19 May 1993 12:44:13 -0500 Return-Path: Received: from sybase.com (sybgate.sybase.com) by halon.sybase.com (5.0/SMI-SVR4/SybFW4.0) id AA25912; Wed, 19 May 93 10:46:19 PDT Received: from ralph.sybgate.sybase.com by sybase.com (4.1/SMI-4.1/SybH3.2) id AA00781; Wed, 19 May 93 10:45:47 PDT Received: from Mac-mailer ([130.214.134.16]) by ralph.sybgate.sybase.com (4.1/SMI-4.1/SybEC3.1) id AA13298; Wed, 19 May 93 10:45:44 PDT Message-Id: <9305191745.AA13298@ralph.sybgate.sybase.com> Date: Wed, 19 May 93 10:46:43 From: jamiel@sybase.com (Jamie Lawrence) To: Subject: Re: V1.50b51 beta and Filter plug-ins Content-Length: 202 Hi- probably a FAQ, but is there any way to access this file through mail? I have no ftp access, and according to the index, the most recent version available through remailing is 1.49. thanks jamie From wayne@zippy.nimh.nih.gov Wed May 19 13:05:22 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA07558; Wed, 19 May 1993 13:05:40 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA19950; Wed, 19 May 93 14:17:05 -0400 Message-Id: <9305191817.AA19950@zippy.nimh.nih.gov> Date: Wed, 19 May 1993 13:05:22 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: FTP mail service >probably a FAQ, but is there any way to access this file through mail? >I have no ftp access, and according to the index, the most recent >version available through remailing is 1.49. You can use the ftp mail service provided by decwrl.dec.com to get files from zippy.nimh.nih.gov. Create an Email message with the following contents: reply chunksize 45000 connect zippy.nimh.nih.gov dir /pub/image quit Send this message to ftpmail@decwrl.dec.com. You'll get back a directory listing. Then you can substitute "get /pub/image/[filename]" (where [filename] is the name of the file you want) in place of the dir line. The file will be broken into messages with a maximum size of 45000 bytes. To get the latest image beta you would use the command "get /pub/image/image150_beta51.hqx". For details on using this ftp mail server, the following message will get you the help file: reply chunksize 45000 help quit --wayne From wayne@zippy.nimh.nih.gov Wed May 19 14:04:14 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA07869; Wed, 19 May 1993 14:04:32 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA20056; Wed, 19 May 93 15:15:57 -0400 Message-Id: <9305191915.AA20056@zippy.nimh.nih.gov> Date: Wed, 19 May 1993 14:04:14 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: automatic fileprocessing >I would like to use Image to process several files during the night. >I understood that this is possible and wonder if one of you could give >me an example how it can be done. I would like to do the following things: >-background correction (e.g. 2d ball) >-sharpening >-thresholding ( is it possible to do this automatically, e.g. with >Isodata threshold method?) >-skeletonizing the binary picture >-counting the number of pixels in the skeletons (would it also be >possible to discard skeletons smaller than e.g. 5 pixels?. Something like the enclosed macro should do the job. --wayne macro 'Process Files '; var n,nFiles,i,PixelCount:integer; begin nFiles:=GetNumber('Number of Files:',1); SetUser1Label('Count'); for n:=1 to nFiles do begin Open(n:3); {Names must be in the form '001','002, etc.} SubtractBackground('2D Rolling Ball',25); Sharpen; AutoThreshold; ApplyLUT; Skeletonize; SetParticleSize(5,999999); AnalyzeParticles; PixelCount:=0; for I:=1 to rCount do PixelCount:=PixelCount+rArea[i]; Dispose; rUser1[n]:=PixelCount; {Save count in user1} end; SetCounter(n); ShowResults; end; From wayne@zippy.nimh.nih.gov Thu May 20 11:41:31 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA12379; Thu, 20 May 1993 11:42:01 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA21271; Thu, 20 May 93 12:53:25 -0400 Message-Id: <9305201653.AA21271@zippy.nimh.nih.gov> Date: Thu, 20 May 1993 11:41:31 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Think C 4.0 example plug-ins I have modified the source for the example acquisition and filter plug-ins on Zippy so they compile with Think C 4.0. The code is in the file developers_kit.cpt.hqx(a self-extracting archive) in the plug-ins directory. To get them to compile, I had to through out most of the includes, remove the definition of 'nil' and rebuild the projects from scratch. I also included the executables so people without Think C can try them out. --wayne From sriram@owlnet.rice.edu Thu May 20 07:06:34 1993 Received: from moe.rice.edu by nx1.soils.umn.edu (5.65c) id AA12561; Thu, 20 May 1993 12:05:08 -0500 Return-Path: Received: from owlnet.rice.edu by moe.rice.edu (AA17638); Thu, 20 May 93 12:06:37 CDT Received: from flammulated.owlnet.rice.edu by owlnet.rice.edu (AA27202); Thu, 20 May 93 12:06:34 CDT Received: by flammulated.owlnet.rice.edu (AA26226); Thu, 20 May 93 12:06:34 CDT Date: Thu, 20 May 93 12:06:34 CDT From: Sriram Neelamegham Message-Id: <9305201706.AA26226@flammulated.owlnet.rice.edu> To: nih-image@soils.umn.edu Subject: pre defined arrays Wayne, What changes do i have to make to the image source code in order that i am able to introduce some more pre defined arrays.... say rUser3 and rUser4? I have made some changes to macro2.p and to global.p. I thik there are a few more modifications i need to make. Sriram Neelamegham Rice University, Houston From mark@hephaestus.earth.monash.edu.au Fri May 21 05:03:57 1993 Received: from monu1.cc.monash.edu.au by nx1.soils.umn.edu (5.65c) id AA14603; Thu, 20 May 1993 19:03:48 -0500 Return-Path: Received: from hephaestus.earth.monash.edu.au by monu1.cc.monash.edu.au (5.61/1.34) id AA23804; Fri, 21 May 93 10:05:11 +1000 Received: by hephaestus.earth.monash.edu.au (4.1/SMI-4.1) id AA15087; Fri, 21 May 93 10:03:57 EST Date: Fri, 21 May 93 10:03:57 EST From: mark@hephaestus.earth.monash.edu.au (Mark Jessel) Message-Id: <9305210003.AA15087@hephaestus.earth.monash.edu.au> To: nih-image@soils.umn.edu Subject: plugins for think c ver 5.0.4 We have compiled the dissolve Plug In under think C ver 5.0.4 What follows is the results from a 'compare' of the original Plug In and the Think C ver 5.0.4 version File A = Dissolve.c orig File B = Dissolve.c Match Criterion = 6 lines. *********************************** Mismatch: File A, Lines 9 thru 24: * #include * #include * #include * #include * #include * #include * #include * #include * #include * #include * #include * #include * * #include "FilterInterface.h" * * #define nil 0L File B, Lines 9 thru 22: * #include * #include * #include * #include * #include * #include * #include * #include * #include * #include * #include * * #include "FilterInterface.h" * I hope this may be of help to someone. Also you must remove the old MACTRAPS library and add the think C v 5.0.4 ones. Cheers Andrew Matthews & Mark Jessell From rjb1@cec2.wustl.edu Fri May 21 03:41:01 1993 Received: from cec2.wustl.edu by nx1.soils.umn.edu (5.65c) id AA17388; Fri, 21 May 1993 08:40:10 -0500 Return-Path: Received: by cec2.wustl.edu (5.65a/1.35); id AA03305; Fri, 21 May 93 08:41:01 -0500 Date: Fri, 21 May 93 08:41:01 -0500 From: rjb1@cec2.wustl.edu (Randall John Bateman) Message-Id: <9305211341.AA03305@cec2.wustl.edu> To: nih-image@soils.umn.edu Subject: Muscle content in image Hi everyone, I have just received a copy of image and I am trying to use it to give a quantitative value of the amount of muscle shown on a cross section MRI scan of the subjects' legs. I am also having problems getting the program to load my graphics files. The files are from a GE MRI and were put on a quadra 950 using ximage software provided by GE. The images were tape backuped using tar(is there a setting for binary using this?) and uploaded to our network using another Sun Sparcstation. Now, the quadra has access to the files on the network, but each file is different in size(by about 600 bytes). Since each file is the same size(512 by 292) I thought they should be the same size. If anyone out there knows how to transfer the files correctly, or how to load them once they are transferred or knows how to get a quantitative answer for the cross sectional area of the muscle(which is darker than fat, bone, etc.) your help would be greatly appreciated. Sincerely, Randall Bateman Mass Spectrometry Lab @ Washington University From @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Fri May 21 07:28:41 1993 Received: from VILL.EDU (wild.ucis.vill.edu) by nx1.soils.umn.edu (5.65c) id AA17833; Fri, 21 May 1993 10:14:56 -0500 Resent-From: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Message-Id: <199305211514.AA17833@nx1.soils.umn.edu> Return-Path: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Date: Fri, 21 May 1993 11:21 EST Received: from vu-vlsi.ee.vill.edu [153.104.63.20] by VILL.EDU with SMTP-VMS via TCP/IP; Fri, 21 May 1993 11:21 EST Received: by vu-vlsi.ee.vill.edu (4.1/SMI-4.1a) id AA18711; Fri, 21 May 93 11:28:41 EDT From: mobasser@vu-vlsi.ee.vill.edu (Bijan Mobasseri) Message-Id: <9305211528.AA18711@vu-vlsi.ee.vill.edu> Subject: Flame color and its temperature To: nih-image@soils.umn.edu Date: Fri, 21 May 1993 11:28:41 -0400 (EDT) X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 558 I have noticed quite a few chemists on this net so this question may be appropriate here. I need to know if there is a correspondence between the color of a flame and its temperature. In other words, can you do a color analysis on video clips of a burner and come up with some quantitative temperature map?. If there is no one to one mapping, what are the options in determining such a map using image analysis techniques. Would IR serve as an alternative technique?. Thanks in advance. Bijan Mobasseri Computer Vision Laboratory Villanova University From wayne@zippy.nimh.nih.gov Fri May 21 13:39:14 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA18724; Fri, 21 May 1993 13:39:43 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA23610; Fri, 21 May 93 14:51:01 -0400 Message-Id: <9305211851.AA23610@zippy.nimh.nih.gov> Date: Fri, 21 May 1993 13:39:14 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Mac Image Processing Packages The following summary of Mac image processing programs was posted to the sci.image.processing newsgroup on Usenet by Peter Clinch of the University of Dundee, Scotland. --wayne There seem to be several people out there who want to know about Mac Packages, so here's an edited list of replies I kept... ------------------------------------------------------------------------ I'm pretty happy with a package called DipStation. I think you can also make user code modules that are basically written in c. dip->contact = William H. Conner whc@po.cwru.edu (216) 721-2388 (USA) BTW. My only complaint is lack of user support. The manual says that they send out demo disk, and if you call to get one, tell him there's someone at UNC that emailed him about 3d render that would like to talk to him. ------------------------------------------------------------------------ As far as I know, there are two image processing applications available on the Mac - one is freeware and the is a commercial program. The freeware is NIH Image - the latest version of which is available via anonymous FTP at many Mac software sites (try SUMEX-AIM.STANFORD.EDU, WUARCHIVE.WUSTL.EDU, or MAC.ARCHIVE.UMICH.EDU). This is a great program to get started with on the Mac. It supports several common image-grabber boards and has a reasonably comprehensive set of image manipulation/ analysis routines as part of the basic code. It has a limited, but useful, macro building ability to automate collections of commands. You can extend its functionality, but I think you have to use Pascal, not C. The program itself is written in Pascal. The commercial program is IPLab and is written and supported by a company called Signal Analytics. They are located somewhere in Virginia, USA - I'm at home now and don't have a manual readily available. They are available over the Internet via AppleLink. This is a package that we've used extensively over the last year to automate the analysis of immunofluorescence images of tissue sections. It has a relatively flexible macro building capability that we've used often. IPLab contains hooks which enable you to create code extensions in Think C/Pascal or MPW C/Pascal. We've done all our programming in Think C (from Symantec) and have had very good luck getting the program to do what we want it to do. NIH Image is a wonderful program written and supported by a very talented programmer at the NIH, but IPLab has a whole company working to expand it. They advertise it as a scientific imaging tool, so they are keyed into the needs of research imaging systems. They have also recently hired a biophyicist to help tailor IPLab for use on ratio imaging systems (ion sensitive dye measurements in single cells - e.g., FURA, BCECF,etc.). I have had a great deal of personal contact with them while writing code extensions for IPLab, and they have been very helpful. They are also available as consultants for a fee, so if you have a specific functionality you need to add to the program, you can hire them to do it for you. The program itself contains extensive image processing routines; we have rarely had to resort to writing our own code. When we have, it was to do something trivial. They have very efficient versions of most of the standard image processing filters already written into the basic package. The program also supports a large array of frame-grabber boards and a very powerful set of NuBus Image array processing boards (made by a company called Perceptics). I believe the current price for a single user version of IPLab is ~$1200. For another $120/year, you get free bi-annual upgrades and unlimited support services via phone (not very practical for you) or Internet (they always get back to me within two days when I send them e-mail). P.S.: I found my annual product guide issue of Science. Here's Signal Analytics vital stats: Signal Analytics Corp. 374 Maple Ave, East Suite 204 Vienna, VA 22180 (703) 281-3277 (703) 281-2509 [FAX] You know, there are a few other packages available for the Mac. I've forgotten about them, because we've become so attached to IPLab. One is by a company called Biological Detection Systems in Pittsburgh,PA. They are a company that has been around in one form or another for the last 5 years or so. It was formed to capitalize on technology developed at Carnegie-Mellon's Center for Fluorescence Studies. The package they sell is a turnkey system that comes with a computer. The main product is the software, however, and includes such niceties as removal of out-of-focal-plane light to de-blur images and 3-D reconstruction of a spatial series of images. Their software is at least $10,000 for the basic package, and it can be much more than that depending on how many of their add-ons you buy. Axon Instruments [(415)571-9500 {FAX}] and Jandel Scientific [(800) 874-1888] both have general purpose Mac imaging packages available, but I don't know anything about them, because they have come out in the last year, after we bought IPLab. The most widely available commercial Mac imaging software is a package tailored for use in computer-aided manufacturing. It is designed to interface well with video input devices that can be used to monitor and evaluate the manufacturing process. The name of this system slips my mind - I think it's Image Analyst -, but it is also more expensive than IPLab, and not quite tailored to the needs of basic research. It uses a set of libraries called MacRail which were written specifically for imaging processing and video control systems. Finally, the National Center for Supercomputing Applications at the University of Illinois, Urbana-Champaign has created a large variety of data visualization tools and are starting to do more direct work with image processing. Most of their packages are available in commercial form(and some as freeware) for the Mac. They would be a good source for advice, as well. ------------------------------------------------------------------------ Currently I am using "Image Analyst" by Automatix for image processing in fluid mechanics. Seems to work quite well. I have a RasterOps frame grabber in my Mac IIfx and the images are taken directly from a video source. There is a version called "Image Analyst/Source" which has a high level developers environment incorporated into the package. It supports user-written C programs and Automatix C libraries. As well it uses a generic macro language called RAIL as an interpretive image processing and control language. Contacts are Automatix Inc. 755 Middlesex Turnpike Billerica, MA 01821 508/667 7900, Fx. 508/663-5482, or Automatix Int. UK Ltd. Fairfield Court Unit 2 Wheler Road Coventry CV3 4LJ 44 203 639609, fx. 44 203 639 010 ------------------------------------------------------------------------ NIH Image is the "standard" free software environment for Macs. I do not know if it is extendable in C. Image Analyst is a very good commerical package, particularly for dimensional measurements. However you write in an intrepreted lanuage call MacRAIL. I can provide a contact if you want further information. Alternatively you could use the Mac as a X terminal and run a UNIX based image processing routine which provides the features your require. I use Khoros. Khoros, by the way, runs on Mac's with AUX and X11. ------------------------------------------------------------------------ Biological Detection Systems has a system like this: BDS-Image. Very powerful, aimed at the microscopy market. You should call our sales people at 301-990-0100 (USA) and ask for some information, or write to: Biological Detection Systems 15200 Omega Drive, Suite 105 Rockville MD 20850 Phone (301) 990-0100 FAX (301) 990-8391 ------------------------------------------------------------------------ Also, National Instuments LabView for Mac also has many function which can be used for IP. Similarly, you could check out Momentum Data Systems at (714) 557-6884. ------------------------------------------------------------------------ I've taken out some of the repeated information. Thanks to all those who sent it to me, and I hope people find this useful. Pete. p.s. sorry for the blip at the start, with this as a followup rather than the posting, but if I say I'm using vi I'm sure you'll understand :-) -- Peter Clinch University of Dundee phone 44 382 (voice) 60111 x 2050 (fax) 632970 Department of Medical Physics email pjclinch@dux.dundee.ac.uk Ninewells Hospital Dundee, DD1 9SY, Scotland, UK From tom@otter.biochem.ubc.ca Fri May 21 05:11:54 1993 Received: from hub.ubc.ca by nx1.soils.umn.edu (5.65c) id AA18911; Fri, 21 May 1993 14:16:59 -0500 Return-Path: Received: from otter.biochem.ubc.ca by hub.ubc.ca (4.1/1.14) id AA06700; Fri, 21 May 93 12:18:26 PDT Received: by otter.biochem.ubc.ca (920330.SGI/920502.SGI.AUTO) for @hub.ubc.ca:nih-image@nx1.soils.umn.edu id AA12674; Fri, 21 May 93 12:17:29 -0700 Date: Fri, 21 May 1993 12:11:54 -0700 (PDT) From: tom jovin Subject: Re: Mac Image Processing Packages To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <9305211851.AA23610@zippy.nimh.nih.gov> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII There are two other image processing programs that can run on Mac platforms (as well as on SUN, PC, etc.). They were developed at the Technical University of Delft and in the University ofb Amsterdam and in my estimation provide the greatest functionality of any other systems known to me. I use these programs in combination with NIH-Image because the latter program has the best implementation of cursor-driven functions (but lacks certain essential features, for example the ability to deal with 16-bit or floating formats). The programs in question are TCL-Image and SCIL-Image. They (or at least TCL-Image) are distributed by Perceptics in the US (or was) and in Europe by Multihouse (Amsterdam). There are brand new revisions of these programs with significant enhancements. tom jovin From SBK111@SBMS01.MSRC.SUNYSB.EDU Sun May 23 20:05:00 1993 Received: from sbms01.msrc.sunysb.edu by nx1.soils.umn.edu (5.65c) id AA29510; Sun, 23 May 1993 23:30:30 -0500 Return-Path: Date: Mon, 24 May 93 00:05 EDT From: Barr Kum Subject: Re: Mac Image Processing Packages To: nih-image@soils.umn.Edu Message-Id: <30B0570F789F804081@SBMS01.MSRC.SUNYSB.EDU> X-Organization: Marine Sciences Research Center X-Envelope-To: nih-image@soils.umn.Edu X-Vms-To: IN%"nih-image@soils.umn.Edu" X-Vms-Cc: SBK111 Someone had asked a question on color mapping temperature (Ive deleted the queston). I imagine techniques where Sea Surface Temperature is mapped using IR would be applicable. The general outline is 1) Normalise the data which will include transmission path, angle of deection 2) Calibrate the data to known temperature 3) All data afterwards that is normalised can be used to measure temperature Send me e-mail if you need any details or references Barr-Kum From @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Mon May 24 05:15:58 1993 Received: from VILL.EDU (wild.ucis.vill.edu) by nx1.soils.umn.edu (5.65c) id AA01221; Mon, 24 May 1993 08:01:28 -0500 Resent-From: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Message-Id: <199305241301.AA01221@nx1.soils.umn.edu> Return-Path: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Date: Mon, 24 May 1993 09:07 EST Received: from vu-vlsi.ee.vill.edu [153.104.63.20] by VILL.EDU with SMTP-VMS via TCP/IP; Mon, 24 May 1993 09:07 EST Received: by vu-vlsi.ee.vill.edu (4.1/SMI-4.1a) id AA28273; Mon, 24 May 93 09:15:58 EDT From: mobasser@vu-vlsi.ee.vill.edu (Bijan Mobasseri) Message-Id: <9305241315.AA28273@vu-vlsi.ee.vill.edu> Subject: Color and temperature To: nih-image@soils.umn.edu Date: Mon, 24 May 1993 09:15:58 -0400 (EDT) In-Reply-To: <30B0570F789F804081@SBMS01.MSRC.SUNYSB.EDU> from "Barr Kum" at May 23, 93 11:37:38 pm X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 644 > > > Someone had asked a question on color mapping temperature (Ive deleted the > queston). I imagine techniques where Sea Surface Temperature is mapped > using IR would be applicable. > The general outline is > 1) Normalise the data which will include transmission path, angle of deection > 2) Calibrate the data to known temperature > 3) All data afterwards that is normalised can be used to measure temperature > > Send me e-mail if you need any details or references > > Barr-Kum > ------------- Yes, I would appreciate any references on IR imaging/photogrpahy. You may post here or directly to mobasser@vu-vlsi.ee.vill.edu. Bijan From rjb1@cec2.wustl.edu Mon May 24 04:50:35 1993 Received: from cec2.wustl.edu by nx1.soils.umn.edu (5.65c) id AA01843; Mon, 24 May 1993 09:49:50 -0500 Return-Path: Received: by cec2.wustl.edu (5.65a/1.35); id AA07163; Mon, 24 May 93 09:50:35 -0500 Date: Mon, 24 May 93 09:50:35 -0500 From: rjb1@cec2.wustl.edu (Randall John Bateman) Message-Id: <9305241450.AA07163@cec2.wustl.edu> To: nih-image@soils.umn.edu Subject: Cross sectional area I wrote several days ago concerning the image software and I questioned how to use it to find the cross sectional area of muscle(the darker pixels of the image). If anyone knows of any way to do this, please let me know. Thanks, Randall Bateman From wayne@zippy.nimh.nih.gov Mon May 24 15:15:31 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA03603; Mon, 24 May 1993 15:15:59 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA26483; Mon, 24 May 93 16:27:24 -0400 Message-Id: <9305242027.AA26483@zippy.nimh.nih.gov> Date: Mon, 24 May 1993 15:15:31 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Cross sectional area >I wrote several days ago concerning the image software and I questioned >how to use it to find the cross sectional area of muscle(the darker pixels >of the image). If anyone knows of any way to do this, please let me know. First use Set Scale to spatially calibrate the image so that the Measure command will report areas in real-world units such as square millimeters. Then try using thresholding to isolate the muscle from the rest of the image. Do this by double-clicking on the LUT tool and manipulating the red bar in the LUT window until the muscle is highlighted in red. If this works, use the Measure command and it will report the area of the red portion of the image(i.e., the muscle). If this doesn't work, use the line selection tool to manually outline the muscle. Read the description of the line selection tool in the manual to find out how to create irregular selections. --wayne From huff@MCCLB0.MED.NYU.EDU Mon May 24 12:33:15 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA04217; Mon, 24 May 1993 16:31:59 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYK8HF4R3K000EOS@MCCLB0.MED.NYU.EDU>; Mon, 24 May 1993 17:33:13 EDT Date: Mon, 24 May 1993 17:33:15 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Re: Mac Image Processing Packages To: nih-image@soils.umn.edu Message-Id: <01GYK8HFKAIA000EOS@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >I'm pretty happy with a package called DipStation. I think you can also >make user code modules that are basically written in c. > > dip->contact = William H. Conner > whc@po.cwru.edu > (216) 721-2388 (USA) > The new address as of last summer is 303-939-0067 HIPG P.O. Box 3092 Boulder Colorado 80303-3092 -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From rjb1@cec2.wustl.edu Mon May 24 12:34:51 1993 Received: from cec2.wustl.edu by nx1.soils.umn.edu (5.65c) id AA04415; Mon, 24 May 1993 17:34:05 -0500 Return-Path: Received: by cec2.wustl.edu (5.65a/1.35); id AA09554; Mon, 24 May 93 17:34:51 -0500 Date: Mon, 24 May 93 17:34:51 -0500 From: rjb1@cec2.wustl.edu (Randall John Bateman) Message-Id: <9305242234.AA09554@cec2.wustl.edu> To: nih-image@soils.umn.edu Subject: Re: Cross sectional area Thanks for all of your help. I just got the image to load on the program after excluding the file header info. It is a 256 by 256 matrix and is 16 bit signed. I have been able to get the results I needed. Once again, thanks all. -Randall Bateman Washington University Mass Spect. Lab P.S. If anyone works with a GE MRI and needs any help porting the image, just write me: rjb1@cec2.wustl.edu P.P.S. The ximage is a util to extract the image portion out of a database that can't be accessed otherwise and was kindly supplied by GE. From huff@MCCLB0.MED.NYU.EDU Mon May 24 14:57:26 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA04692; Mon, 24 May 1993 18:56:08 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYKDI7B5O00009S4@MCCLB0.MED.NYU.EDU>; Mon, 24 May 1993 19:57:24 EDT Date: Mon, 24 May 1993 19:57:26 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Improved PC-VCR support To: nih-image@soils.umn.edu Message-Id: <01GYKDI7LVGY0009S4@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT X-Sender: huff@mcclb0.med.nyu.edu (Unverified) I recently prepared a video tape which displayed slides from one stack, and then stopped the tape, loaded a large stack, displayed it as a movie, etc. WITH automatic centering of the window and hiding everything except the title bar (i.e. almost as good as photo mode). The source changes and macro file are in the contrib directory on zippy. (Source changes are displayed as output of MPW compare against the original 1.49 source), in a text file named video_presentation_1_49.txt or something close to that. Two macros were added: one to start or end "photo mode" (except title bar of window is still shown), and another to resize a window. The resize window macro might not be completely robust, i.e. you might be able to cause a crash with bad parameters, but it got the job done. If you hit command period while the macro is running, the display is a little confusing, since the menu bar stays hidden until you do something that redraws it. This could be fixed by adding a check at macro exit for an unterminated photo mode macro. -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From sarmienu@rnisd0.DNET.roche.com Tue May 25 03:19:25 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA06377; Tue, 25 May 1993 06:18:17 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA10126; Tue, 25 May 93 07:19:28 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA09378; Tue, 25 May 93 07:17:32 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9305251117.AA09378@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Tue, 25 May 93 07:19:25 EDT Date: Tue, 25 May 93 07:19:25 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: RE: Cross sectional areas >I wrote several days ago concerning the image software and I questioned >how to use it to find the cross sectional area of muscle(the darker pixels >of the image). If anyone knows of any way to do this, please let me know. Perhaps my limited experience with image analysis would be of some help here: 1) Establish a scale depending on the magnification of your video source (see manual) 2) Play with the video control chanels to select the one that best highlights my object of interest. 3) Use the density slice command to identify (select?) the particle(s) of interest according to its (their) level of grey. 4) Selecting "options" from the "analyze" menu, make sure that "area" is selected. Then select "measure" from the same menu (If there is only one particle of interest) or "analyse particles" (if more than 1). 5) You can also use the "magic wand" set at automeasure (from the options dialogue box, "analyze menu") and go clicking on the particles of interest, if you want to discriminate particles. 6) To determine the area of the field of interest (the Image window), select all and then measure. This is in case you want to determine a ratio of # or particles per field. 7) If you use the video control, you might select areas contained into other areas and discriminate them using the chanels as filters of colors to deemphasize or emphasize particles. In the "options" menu you may select "ignore particle holes" if the particle is doughnut-shaped. Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 From wayne@zippy.nimh.nih.gov Tue May 25 08:11:32 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA06746; Tue, 25 May 1993 08:12:01 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA27245; Tue, 25 May 93 09:23:26 -0400 Message-Id: <9305251323.AA27245@zippy.nimh.nih.gov> Date: Tue, 25 May 1993 08:11:32 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Improved PC-VCR support >Two macros were added: one to start or end "photo mode" (except title bar >of window is still shown), and another to resize a window. The resize >window macro might not be completely robust, i.e. you might be able to >cause a crash with bad parameters, but it got the job done. If you hit >command period while the macro is running, the display is a little >confusing, since the menu bar stays hidden until you do something that >redraws it. This could be fixed by adding a check at macro exit for an >unterminated photo mode macro. v1.50b52(on Zippy) has a macro command for enabling and disabling "photo mode". It also has a check at macro exit for an unterminated photo mode macro. --wayne From eran@fnth06.fnal.gov Tue May 25 11:40:28 1993 Received: from fnbit.fnal.gov by nx1.soils.umn.edu (5.65c) id AA08717; Tue, 25 May 1993 16:39:20 -0500 Return-Path: Received: from fnth06.fnal.gov ([131.225.10.4]) by fnbit.fnal.gov with SMTP; Tue, 25 May 1993 16:40:42 -0500 (CDT) Received: by fnth06.fnal.gov (NX5.67c/NX3.0S) id AA07640; Tue, 25 May 93 16:40:30 -0500 From: Eran Yehudai Message-Id: <9305252140.AA07640@fnth06.fnal.gov> Subject: Dumping a table of actual vs. calibrated grey levels To: nih-image@soils.umn.edu Date: Tue, 25 May 93 16:40:28 CDT X-Mailer: ELM [version 2.3 PL0] Does anyone know of a simple method (macro or otherwise) of dumping a table of actual vs. calibrated grey levels (with actual levels taking every value from 0 to 255)? Thanks, Eran Yehudai From wayne@zippy.nimh.nih.gov Tue May 25 16:48:41 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA08829; Tue, 25 May 1993 16:49:10 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA28338; Tue, 25 May 93 18:00:36 -0400 Message-Id: <9305252200.AA28338@zippy.nimh.nih.gov> Date: Tue, 25 May 1993 16:48:41 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Dumping a table of actual vs. calibrated grey levels >Does anyone know of a simple method (macro or otherwise) of dumping a >table of actual vs. calibrated grey levels (with actual levels taking >every value from 0 to 255)? I have enclosed a macro(it's from the file "Measurement Macros") that generates a table of actual vs. calibrated values. --wayne macro 'Display Calibration Table'; { Stores 0-255(all possible gray values) in the User1 column and the 256 corresponding calibrated values in the User2 column. Max Measurements must be set to 256 or greater. Use the Export command to export the calibration table to a text file. The two columns will be identical if the image is not calibrated. } var i:integer; v:real; begin RequiresVersion(1.44); SetCounter(256); SetUser1Label('value'); SetUser2Label('cvalue'); for i:=0 to 255 do begin rUser1[i+1]:=i; rUser2[i+1]:=cvalue(i); end; ShowResults; end; From bonnot@jouy.inra.fr Wed May 26 13:27:29 1993 Received: from chenas.inria.fr by nx1.soils.umn.edu (5.65c) id AA11055; Wed, 26 May 1993 06:20:23 -0500 Return-Path: Received: from inra.inra.fr by chenas.inria.fr (5.65c8d/92.02.29) via Fnet-EUnet id AA20375; Wed, 26 May 1993 13:21:36 +0200 (MET) Received: from jouy.jouy.inra.fr by inra.inra.fr, Wed, 26 May 93 13:19:57 +0200 Received: from [134.214.100.195] (insabio1.univ-lyon1.fr) by jouy.jouy.inra.fr, Wed, 26 May 93 13:16:51 +0200 Message-Id: <9305261116.AA14334@jouy.jouy.inra.fr> Date: Wed, 26 May 1993 13:27:29 +0000 To: From: bonnot@jouy.inra.fr Subject: Re: V1.50b51 beta and Filter plug-ins >*Supports Photoshop filter plug-ins >*Uses submenus for plug-ins >Please let me know what acquisition and filter plug-ins seem to work so I >can add them to the list in the Change History file. > We bought (before our discovery of Image) a QuickImage 24 Video frame graber card from MASS Microsystems. This card comes with its own driver and a Photoshop plug-in QI/Photoshop v 1.6 is also provided . The Plug-in allows capture from within Photoshop as well as from Image but in a blind way because the video preview does not work in both cases (the preview window is awfully unstable). As the ability to capture frames from Image is interesting for us, I shall ask to the card seller to find a solution to this problem. ######################################################## # Guy Bonnot phone: +33 - 72 43 83 56 # # INRA - INSA fax: +33 - 72 43 85 11 # # Biologie 406 internet: bonnot@lyon.inra.fr # # 20, av. A. Einstein =bonnot@jouy.inra.fr # # 69621 Villeurbanne cedex # # France United Europe # ######################################################## From Carl_Gustafson@cbis.ece.drexel.edu Wed May 26 06:42:38 1993 Received: from cbis.ece.drexel.edu by nx1.soils.umn.edu (5.65c) id AA11139; Wed, 26 May 1993 06:42:38 -0500 Return-Path: Received: from homer.ece.drexel.edu by cbis.ECE.Drexel.EDU (5.67/1.34) id AA15107; Wed, 26 May 93 07:43:52 EDT Message-Id: <9305261143.AA15107@cbis.ECE.Drexel.EDU> Date: 26 May 1993 07:43:01 U From: "Carl Gustafson" Subject: Plug-ins To: "image listserv " Subject: Time:7:41 AM OFFICE MEMO Plug-ins Date:5/26/93 I have tried Image 1.50b48 with the Agfa PhotoScan 2.12 plugin (for the Arcus scanner). Works fine; you can add it to the list of acquisition plug-ins. From John_Mansfield@mse.engin.umich.edu Wed May 26 07:00:17 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA11219; Wed, 26 May 1993 07:00:17 -0500 Return-Path: Message-Id: <199305261200.AA11219@nx1.soils.umn.edu> Date: 26 May 1993 08:01:54 U From: "John Mansfield" Subject: Re: Plug-ins To: nih-image@soils.umn.edu Reply_ RE>Plug-ins Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu If you try all the fancy filters that come with Photoshop 2.0.1, you get the fairly predictable dialog saying: "This filter requires features of Photoshop 2.0" or something similar. Too bad :-( From kartenh@Sdsc.Edu Wed May 26 16:04:30 1993 Received: from Sdsc.Edu (m5.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA12024; Wed, 26 May 1993 11:03:22 -0500 Return-Path: Date: Wed, 26 May 93 16:04:30 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930526160430.204044b1@m5.sdsc.edu> Subject: RE: Plug-ins To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Is there a Plug-In for the HP Scanners? Harvey Karten Harvey J. Karten, M.D. Dept. Neurosciences UCSD 0608 La Jolla, CA 92093 Phone (619)-534-4938 FAX (619)-534-6602 E-Mail KARTENH@SDSC.EDU From wayne@zippy.nimh.nih.gov Wed May 26 11:30:05 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA12191; Wed, 26 May 1993 11:30:44 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA29661; Wed, 26 May 93 12:42:01 -0400 Message-Id: <9305261642.AA29661@zippy.nimh.nih.gov> Date: Wed, 26 May 1993 11:30:05 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: RE: Plug-ins >Is there a Plug-In for the HP Scanners? A recent issue of MacWorld(or perhaps MacUser) had a review of scanners and the HP Scanjet was the only scanner tested that did not come with a Photoshop plug-in. --wayne From JCAIRA@UCONNVM.BITNET Sun May 26 07:53:09 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA12361; Wed, 26 May 1993 11:55:08 -0500 Return-Path: Received: from UCONNVM.UCONN.EDU (MAILER@UCONNVM) by vx.cis.umn.edu (PMDF #2574 ) id <01GYMPA1Q96O9GWXBO@vx.cis.umn.edu>; Wed, 26 May 1993 11:56:35 CDT Received: from UCONNVM (JCAIRA) by UCONNVM.UCONN.EDU (Mailer R2.08 R208004) with BSMTP id 8797; Wed, 26 May 93 12:53:56 EST Date: 26 May 1993 12:53:09 -0500 (EST) From: JCAIRA@UCONNVM.BITNET To: nih-image@soils.umn.edu Message-Id: <01GYMPASNUOC9GWXBO@vx.cis.umn.edu> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT unsubscribe jcaira@uconnvm.uconn.edu From TATE@FAXCSL.DCRT.NIH.GOV Wed May 26 10:55:16 1993 Received: from faxcsl.dcrt.nih.gov by nx1.soils.umn.edu (5.65c) id AA12955; Wed, 26 May 1993 13:56:25 -0500 Return-Path: Date: Wed, 26 May 1993 14:55:16 -0400 (EDT) From: Ramon Tate To: nih-image@soils.umn.edu Message-Id: <930526145516.20a02782@FAXCSL.DCRT.NIH.GOV> Subject: Image Tutorial (and docs in general) As part of an effort to improve our ability to support image processing in our in-house Scientific Computing Resource Center, I've undertaken a collaboration with Wayne Rasband to improve and expand the available Image documentation. At the top of the "To Do" list is a tutorial that will help the novice or occasional Image user do many of the common image processing tasks. To that end, I'm asking for comments on the appended outline for such a Tutorial Manual. Since we believe that good examples are essential, I would also appreciate the contribution of SMALL images that could be used as subject matter for the proposed tutorial categories. HOWEVER, please do not send the images to either of us yet - just a note about what you have to offer will suffice for now; we will be in contact with potential contributors when we determine what we will need. Please reply by email and I will summarize to the mailing list. [Also in the planning: a macro manual and a revised user's reference manual.] ---------------------------------------------------------------------------- Proposed IMAGE Tutorial Manual Elements Starting Up Hardware Considerations Installation Memory Management Tips Image Fundamentals What Is a Digital Image? Calibration Topics Printing Digital Images Tutorials Image Modifications Basic Measurements Gel Image Analysis Particle Measurements ------------------------------------------------------------------------------- Ramon L. Tate, Ph.D. "Eschew equimagnitudinal contraincrementation." Division of Computer - Richard Shrager Research and Technology National Institutes of Health Bethesda, MD 20892 USA tate@faxcsl.dcrt.nih.gov From huff@MCCLB0.MED.NYU.EDU Wed May 26 11:00:28 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA13178; Wed, 26 May 1993 15:00:10 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYMXT3RUHS000LMJ@MCCLB0.MED.NYU.EDU>; Wed, 26 May 1993 16:00:31 EDT Date: Wed, 26 May 1993 16:00:28 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: UserCode interface extension To: nih-image@soils.umn.edu Message-Id: <01GYMXT6VAUE000LMJ@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT The timer support I posted to zippy.nimh.nih.gov in /pub/image/contrib also had a change to the UserCode interface. I have thought about this, and decided on suggesting a different change, for which I have code and will be uploading maybe next week. The proposal is: (1) UserCode takes a variable number of arguments. I implemented this without need to call putback token. (2) The maximum number is of arguments is five: UserCode(selector:integer;p1,p2,p3:real;str:string); (3) Selector value zero is defined to give the correct selector code to use to call a named routine, i.e. Conv16To8 := UserCode(0,0,0,0,'Conv16To8'); Then later write UserCode(Conv16To8,p1,p2,p3); This also has the advantage that if a different version of Image is used, probably the routine names will be different... Users would be prudent to put a version check in so their macros check to see that the proper user.p is included before trying to use usercode. (4) UserFunc(selector:integer,p1,p2,p3:real;str:string):real is also defined. The same selector codes are used, in fact, the same code is executed. (5) UserStr(selector:integer,p1,p2,p3:real;str:string):string is also defined, to permit user code to return a string. Send me any comments, or suggestions for further refinements in design. This version has less runtime overhead than the previous design, and is upward compatible with existing calls to UserCode. Also, from User.p, now you can pass strings back to MacroError. (Since User.p comes before Macros in the build order, MacroError cannot be called From within user.p. I fixed this by putting a var string argument to the Received: from cortex.physiol.su.OZ.AU by nx1.soils.umn.edu (5.65c) id AA14064; Wed, 26 May 1993 19:32:05 -0500 Return-Path: Received: by cortex.physiol.su.oz.au (5.57/Ultrix3.0-C) id AA01396; Thu, 27 May 93 09:06:48 +1000 Date: Thu, 27 May 93 09:06:48 +1000 From: philp@physiol.su.oz.au (Philip Poronnik) Message-Id: <9305262306.AA01396@cortex.physiol.su.oz.au> To: nih-image@soils.umn.edu Subject: calcium ratioing and Image-VDM We have just bought a Perceptics Pipeline board and are trying to start up ratiometric Ca measurments using Image-VDM. This means acquiring two images, subtracting the correpsonding background from each and then dividing one by the other. The problem is that the program as it stands does not do division of two images. Has anyone out there already adapted Image-VDM for such operations or had any experience with such applications? Any help would be much appreciated! From scudder@cpac.washington.edu Wed May 26 11:10:59 1993 Received: from bailey.cpac.washington.edu by nx1.soils.umn.edu (5.65c) id AA14241; Wed, 26 May 1993 20:09:59 -0500 Return-Path: Received: by bailey.cpac.washington.edu (5.65/UW-NDC Revision: 2.4 ) id AA14471; Wed, 26 May 93 18:11:00 -0700 From: Message-Id: <9305270111.AA14471@bailey.cpac.washington.edu> Subject: Re: calcium ratioing and Image-VDM To: nih-image@soils.umn.edu Date: Wed, 26 May 93 18:10:59 PDT In-Reply-To: <9305262306.AA01396@cortex.physiol.su.oz.au>; from "Philip Poronnik" at May 26, 93 5:38 pm X-Mailer: Pine [version 0.9.532] Dumb idea - can you take the log of both images and subtract them, then take the anti-log of the resulting image? Seem you would have to work in reals, not 8-bit integers. In <9305262306.AA01396@cortex.physiol.su.oz.au>, Philip Poronnik writes: > > > We have just bought a Perceptics Pipeline board and are trying to start up > ratiometric Ca measurments using Image-VDM. This means acquiring two images, > subtracting the correpsonding background from each and then dividing one by the > other. The problem is that the program as it stands does not do division of two images. Has anyone out there already adapted Image-VDM for such operations or > had any experience with such applications? > > Any help would be much appreciated! > > From mschindl@Physik.TU-Muenchen.DE Thu May 27 15:45:58 1993 Received: from srv.cip.physik.tu-muenchen.de by nx1.soils.umn.edu (5.65c) id AA15676; Thu, 27 May 1993 06:44:47 -0500 Return-Path: Received: from ss3.cip.physik.tu-muenchen.de by srv.cip.physik.tu-muenchen.de with SMTP id AA19191 for (5.65c/IDA-1.4.4/bs-02); Thu, 27 May 1993 13:45:59 +0200 Received: by ss3.cip.physik.tu-muenchen.de (4.1/SMI-4.1) id AA10840; Thu, 27 May 93 13:45:58 +0200 Date: Thu, 27 May 93 13:45:58 +0200 From: Mathias.Schindl@Physik.TU-Muenchen.DE Message-Id: <9305271145.AA10840@ss3.cip.physik.tu-muenchen.de> To: nih-image@soils.umn.edu Subject: Re: calcium ratioing and Image-VDM In <9305262306.AA01396@cortex.physiol.su.oz.au>, Philip Poronnik writes: > > > We have just bought a Perceptics Pipeline board and are trying to start up > ratiometric Ca measurments using Image-VDM. This means acquiring two images, > subtracting the correpsonding background from each and then dividing one by th e > other. The problem is that the program as it stands does not do division of tw o images. Has anyone out there already adapted Image-VDM for such operations or > had any experience with such applications? > > Any help would be much appreciated! > > I suppose you've got the version 1.44 of Image VDM with your Perceptics board. This program is indeed capable of doing a division between two images: Open the two image-windows and select "Show Paste Control" from the "Windows" menu. Then activate and select the first image (press Command-A), press Command-C to copy the image to the clipboard. Now activate the second image and press Command-V (or select "Paste" from the Edit-menu) to paste the clip- board contents. Finally press the button "Divide" on the Paste Control. Of course the results of your division will be a little bit disappointing because they consist only of the closest integer value in the 0-255 range. You can improve the results by checking the "Scale Math" box in the Paste Control which causes your results to be scaled to fit best in the available range. This is much better, but puts you into problems if you need the absolute values. I don't see any other solution with Image VDM as it is, because Image doesn't support Image buffers with floating point values. For more elegant solutions you would have to do some programming on your own. Therefore you would have to buy the PixelTools Developers Kit from Perceptics which includes the full sources of the Image VDM program and comes for $595. Mathias Schindl Physik-Department TU Muenchen From mhaury@pasteur.fr Thu May 27 17:25:56 1993 Received: from mendel.sis.pasteur.fr by nx1.soils.umn.edu (5.65c) id AA16039; Thu, 27 May 1993 08:24:38 -0500 Return-Path: Received: from [157.99.132.93] by mendel.sis.pasteur.fr with SMTP id AA14258 (5.65c8/IDA-1.4.4 for nih-image@soils.umn.edu); Thu, 27 May 1993 15:25:56 +0200 Date: Thu, 27 May 1993 15:25:56 +0200 Message-Id: <199305271325.AA14258@mendel.sis.pasteur.fr> To: nih-image@soils.umn.edu From: mhaury@pasteur.fr (Matthias Haury) Subject: NIH Image For PC Hallo everybody out there... I am very interested to know whether there exists a NIH Image equivalent for the IBM PC (preferably for Windows). I heard some rumors about it, but I can't find a server to download it. If anybody has information I would really appreciate it. Furthermore, I also heard rumors that there is another image program from the MUNICH University, I would also appreciate if anybody knows about it and can tell me where to obtain it (by FTP). Thanks a lot... Matthias ------------------------ Matthias Haury Institut Pasteur 25 rue du Doctor Roux 75724 PARIS CEDEX 15 Tel: (33)-1-45.68.84.21 Fax: (33)-1-45.68.86.39 EMail: mhaury@pasteur.fr From wayne@zippy.nimh.nih.gov Thu May 27 09:25:51 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA16246; Thu, 27 May 1993 09:26:24 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA01405; Thu, 27 May 93 10:37:49 -0400 Message-Id: <9305271437.AA01405@zippy.nimh.nih.gov> Date: Thu, 27 May 1993 09:25:51 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: NIH Image For PC >I am very interested to know whether there exists a NIH Image equivalent >for the IBM PC (preferably for Windows). I heard some rumors about it, but >I can't find a server to download it. If anybody has information I would >really appreciate it. There is no Windows version of NIH Image and I don't know of any equivalent Windows programs. >Furthermore, I also heard rumors that there is another image program from >the MUNICH University, I would also appreciate if anybody knows about it >and can tell me where to obtain it (by FTP). There is a spinoff of NIH Image called MunichImage. It is available by anonymous FTP from zippy.nimh.nih.gov, in the /pub/image/image_spinoffs directory. --wayne From sarmienu@rnisd0.DNET.roche.com Fri May 28 03:12:37 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA20439; Fri, 28 May 1993 06:11:21 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA22481; Fri, 28 May 93 07:12:40 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA23647; Fri, 28 May 93 07:11:29 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9305281111.AA23647@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Fri, 28 May 93 07:12:37 EDT Date: Fri, 28 May 93 07:12:37 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: Mac-based IMAGE and dedicated Image Systems. This questions have come to mind when comparing the Mac with a dedicated image analysis system marketed by Zeiss named IBAS. Months back Joseph Ayers promised an update to COLOR IMAGE which would make it compatible with a Quadra 950 and that he would post it in the zippy directory. Has this been done yet? Can Image commands be used to direct a microscope stage controller? Is there a way to record a series of menu choices or steps taken in the NIH-IMAGE as you work, rather than in a word processor using the macro language? would a program such as Quickeys accomplish that? and then after done, could you edit the thus created macro?? Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 Sarmienu@RNISD0.DNET.ROCHE.COM From sarmienu@rnisd0.DNET.roche.com Fri May 28 03:14:54 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA20478; Fri, 28 May 1993 06:13:39 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA22494; Fri, 28 May 93 07:14:57 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA23657; Fri, 28 May 93 07:13:01 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9305281113.AA23657@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Fri, 28 May 93 07:14:54 EDT Date: Fri, 28 May 93 07:14:54 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: Re: NIH Image For PC >I am very interested to know whether there exists a NIH Image equivalent >for the IBM PC (preferably for Windows). I heard some rumors about it, but >I can't find a server to download it. If anybody has information I would >really appreciate it. There might be several PC based, relatively inexpensive programs out there. I now that Data translation (used to market an image capturing card for the Mac) is now marketing several cards for PC and Windows-compatible software which is also pretty used-friendly. I decided against it because the IMAGE, in my opinion is far superior in power and ease of use. The IBAS analysis system, by Zeiss, is a PC-based dedicated system with all the equipment you can imaging for image analysis. Unfortunately is extremely expensive and archaic when it comes to utilization. Support, documentation and user friendliness are dismal. Roche image analysis systems is now marketing a PC-Based system for image analysis called Roche Pathology Workstation. As the name indicates, this system is especialized in Pathology morphometry, and probably very expensive. Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 Sarmienu@RNISD0.DNET.ROCHE.COM From pekr@research.novo.dk Fri May 28 15:56:28 1993 Received: from danpost.uni-c.dk by nx1.soils.umn.edu (5.65c) id AA20708; Fri, 28 May 1993 06:51:26 -0500 Return-Path: Received: from nngate.novo.dk (xpsrv1.novo.dk) by danpost.uni-c.dk (5.65c+/1.34) id AA16327; Fri, 28 May 1993 13:52:38 +0200 Received: from nn:.DNA_Node.ARTHUR.DECnet MAIL11D_V3 by nngate.novo.dk (5.57/Ultrix3.0-C) id AA21090; Fri, 28 May 93 13:56:28 +0200 Date: Fri, 28 May 93 13:56:28 +0200 Message-Id: <9305281156.AA21090@nngate.novo.dk> From: pekr@research.novo.dk (Peter Kristensen) To: internet::nih-image@soils.umn.edu Cc: Pekr@nngate.novo.dk Subject: Name of window in macro ? When using IMAGE for counting cells, I would like to make a macro that copies the name of the current window together with the number of cells (value of rCount) onto the clipboard. Does anybody have a suggestion of how to do that ? sincerely Peter Kristensen Novo Nordisk Research Denmark (pekr@research.novo.dk) From sarmienu@rnisd0.DNET.roche.com Fri May 28 04:25:32 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA20875; Fri, 28 May 1993 07:24:29 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA22776; Fri, 28 May 93 08:25:35 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA23906; Fri, 28 May 93 08:24:11 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9305281224.AA23906@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Fri, 28 May 93 08:25:32 EDT Date: Fri, 28 May 93 08:25:32 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: Images via phone There is at least one image analysis system for pathologists out there (one of those $100,000 image analysis systems) which is claimed to be able to transmit, at lightning speed, light microscopy pathology images over the phone to pathologists at the other end. I realize you could probably compress an image with disk doubler or stuffit and send it via modem but, how fast can this be? and is there a way to right a macro to accomplish this?? Can somebody without image but with another program, such as Adobe photoshop get dissent color images in this way? Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 Sarmienu@RNISD0.DNET.ROCHE.COM From wayne@zippy.nimh.nih.gov Fri May 28 13:28:19 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22237; Fri, 28 May 1993 13:28:53 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03916; Fri, 28 May 93 14:40:18 -0400 Message-Id: <9305281840.AA03916@zippy.nimh.nih.gov> Date: Fri, 28 May 1993 13:28:19 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Name of window in macro ? >When using IMAGE for counting cells, I would like to make a macro that >copies the name of the current window together with the number of cells >(value of rCount) onto the clipboard. Does anybody have a suggestion of >how to do that ? >sincerely There is a macro command for setting the window name(SetPicName), but no corresponding routine for getting the window name. There is, however, a routine for copying measurement results to the clipboad(CopyResults). Unfortunately, you can only store numbers in the results table, not text. You could try using sequence numbers for the file names and copying the number and the count to the User1 and User2 arrays. --wayne From wayne@zippy.nimh.nih.gov Fri May 28 13:39:14 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22332; Fri, 28 May 1993 13:39:48 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03977; Fri, 28 May 93 14:51:13 -0400 Message-Id: <9305281851.AA03977@zippy.nimh.nih.gov> Date: Fri, 28 May 1993 13:39:14 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Stage control and macro recording. >Can Image commands be used to direct a microscope stage >controller? Macros can be written to drive stage controllers that use an RS-232 ports, such as the Newport PMC200-P programmable 2-axis motion controller >Is there a way to record a series of menu choices or >steps taken in the NIH-IMAGE as you work, rather than in >a word processor using the macro language? would a >program such as Quickeys accomplish that? and then after >done, could you edit the thus created macro?? Image does not have a built-in macro recorder, but you could probably use QuickKeys to record operations in Image and play them back later. The next 1.50 beta version will have a built-in text editor, eliminating the need to use separate word processor or editor for creating and editing macros. --wayne From John_Mansfield@mse.engin.umich.edu Fri May 28 13:46:27 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA22393; Fri, 28 May 1993 13:46:27 -0500 Return-Path: Message-Id: <199305281846.AA22393@nx1.soils.umn.edu> Date: 28 May 1993 14:47:40 U From: "John Mansfield" Subject: Re: Stage control and macro To: nih-image@soils.umn.edu Reply_ RE>>Stage control and macro Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu The next1.50 beta version will have a built-in text editor, eliminating the need to use separate word processor or editor for creating and editing macros. Excellent Wayne!! Thanks! --wayne ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;28 May 1993 14:43:02 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c065cdd026848; Fri, 28 May 93 13:41:33 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA22355; Fri, 28 May 1993 13:39:58 -0500 Date: Fri, 28 May 1993 13:39:58 -0500 Message-Id: <9305281851.AA03977@zippy.nimh.nih.gov> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: wayne%zippy.nimh.nih.gov@relay.tc.umn.edu (Wayne Rasband) To: Multiple recipients of list Subject: Re: Stage control and macro recording. From mvivino@helix.nih.gov Fri May 28 11:12:13 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA22635; Fri, 28 May 1993 14:10:55 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA15542; Fri, 28 May 93 15:12:13 -0400 Date: Fri, 28 May 93 15:12:13 -0400 Message-Id: <9305281912.AA15542@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: macro recording. > The next >1.50 beta version will have a built-in text editor, eliminating the need to >use separate word processor or editor for creating and editing macros. All of us here at NIH have decided that wayne is too valuable to ever spend time sleeping, or God forbid ever die. We have therefore embarked on a plan to first make several clones of wayne and invent ways of supplying his brain with automatic relaxation so that he (they) needen't ever sleep. Mark From chien@jeeves.UCSD.EDU Fri May 28 05:22:58 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA22752; Fri, 28 May 1993 14:21:41 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA04779 sendmail 5.67/UCSD-2.2-sun via SMTP Fri, 28 May 93 12:22:59 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA27031 to nih-image@soils.umn.edu; Fri, 28 May 93 12:22:58 PDT Date: Fri, 28 May 93 12:22:58 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9305281922.AA27031@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: Re: name of window in macro? Now that the macro language has some string capabilities, would it be possible to add a GetPicName() function? Several times I have thought this would be useful: you could then name windows with processed images after the parent image. For instance, a smoothing macro could take a window called "Face", smooth it, and put the results in "Face Smoothed". Chi-Bin Chien chien@jeeves.ucsd.edu From huff@MCCLB0.MED.NYU.EDU Fri May 28 11:11:27 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA23056; Fri, 28 May 1993 15:10:16 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYPQSG00HS000WVO@MCCLB0.MED.NYU.EDU>; Fri, 28 May 1993 16:11:29 EDT Date: Fri, 28 May 1993 16:11:27 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: RE: macro recording. To: nih-image@soils.umn.edu Message-Id: <01GYPQSGDEQQ000WVO@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >> The next >>1.50 beta version will have a built-in text editor, eliminating the need to >>use separate word processor or editor for creating and editing macros. > Cloning Wayne is one purpose of this list. We need volunteers for the following project. Also, we need some way to deal with multiple people working on the same source code at once. (That may be the hard part). Macro recording is not terribly difficult, given that there is a macro command for every menu item. To do it, we need a global variable "MacroRecorder: Boolean" and a menu item "Start Recorder" which changes to "Stop Recorder" when MacroRecorder is true. Then, for every menu item chosen, in Image.p, add "if MacroRecorder then emitMacro('propercommand');" or emitMacro('{}'); Also, the StartRecorder item prompts for a macro name, and appends to a macro text buffer 'macro ''name'';begin' and StopRecorder appends 'end;'. The major work in the above project is identifying the string which belongs inside the emitMacro call. Of course, adding this feature will make certain shortcomings of the macro language obvious, like the inability to select tools... The emitMacro function itself is done as follows: add a new global variable MacroRecorderTextHandle which points to a block of ram allocated with getBigHandle, and MacroRecorderTextSize and MacroRecorderTextOffset. Start recorder would allocate the handle. Also add a new menu item CopyRecorder which copies the contents of the macro recorder text buffer to the clipboard. emitMacro then just checks to see that the handle exists, checks to see that there is enough space, and copies the text into the buffer. I think this will be worth doing, since it should shorten the learning curve till someone can begin writing macros from scratch. Also, we need online help for writing macros. The online help package from Disinfectant (available by FTP from John Nostrand) would be great. At the moment, I usually keep microsoft word open to the macro appendix or copy that into another text editor... Some user out there ought to put together the text that would go into online help, basically a macro reference: list of all functions in alphabetical order, and in the logical order... -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From norm_hurst@maca.sarnoff.com Fri May 28 15:50:01 1993 Received: from nova (nova.sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA23358; Fri, 28 May 1993 15:50:01 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA23846; Fri, 28 May 93 16:51:14 EDT Message-Id: <9305282051.AA23846@nova> Date: 28 May 1993 16:32:28 U From: "Norm Hurst" Subject: HDTV Grand Alliance Press r To: "NIH Image user group" HDTV Grand Alliance Press release 5/28/93 4:24 PM Advisory Committee On Advanced Television Service For immediate release: May 24, 1993 HDTV "Grand Alliance" Proposal Will be Considered by FCC Advisory Committee Washington, D.C. The Federal Communications Commission's Advisory Committee on Advanced Television Service (established by the Commission in 1987) will review a single digital high definition television (HDTV) system proposed today by a "Grand Alliance" of entities that, until now, had sponsored the four remaining competitive HDTV systems. These entities (AT&T, the David Sarnoff Research Center, General Instrument, Massachusetts Institute of Technology (MIT), North American Philips, Thomson Consumer Electronics, and Zenith Electronics) today reached a business and technical agreement and submitted to the Committee a merged system proposal. The proposed system, if recommended by the Advisory Committee and adopted by the FCC, could place the U.S. in the forefront of high definition video technology. An all-digital standard, which would facilitate interoperability among broadcasting, cable, computer, and telecommunications technologies, has worldwide potential. Advisory Committee Chairman Richard E. Wiley, who had encouraged the complex negotiations leading to the agreement, said "I believe the Grand Alliance proposal, subject to Advisory Committee and ultimate FCC approval, will help to conclude a process that has fostered the development of highly advanced digital HDTV technology. The members of the Alliance should be commended for their accomplishments." Wiley added that the benefits of the Grand Alliance include development of a digital system incorporating the best elements of the four systems and acceleration of HDTV service implementation. The FCC's Advisory Committee endorsed the Alliance concept at a meeting in February. Important aspects of the Grand Alliance technical proposal submitted today include the employment of progressive scan transmission (where entire picture frames are transmitted sequentially) and the use of so-called "square pixels" (where the dots on a television screen are arranged in equally spaced rows and columns). Both of these design aspects are important for the interoperability of HDTV with computers, telecommunications, and other media and applications. Interlaced scan transmission (as deployed in today's TV systems) would also be accommodated in the initial deployment(1). Specifically, the proponents agree that all large-screen HDTV receivers (34 inches in diagonal and above) will incorporate a 60 frame per second 787.5 line or higher progressive scan display mode. Progressive display would be optional initially for smaller screen receivers. The proponents also concur that all transmission of film material will be in a progressive scan format beginning immediately upon the commencement of HDTV service. Finally, the Grand Alliance proponents unanimously endorse the objective of migrating the standard to a high line number (i.e. thousand-line plus) progressive scan transmission as soon as feasible, and will work together to eliminate interlaced scanning format from the transmission path in the future. To support multiple transmission formats, the merged system will feature source adaptive processing. Moreover, to promote system flexibility and extensibility, the merged system also will feature a prioritized, packetized data transport structure. Additionally, the Grand Alliance entities agree to support the Alliance's proposed HDTV compression system in the International Standards Organization as the MPEG-2 HDTV profile. Over the next few weeks, Advisory Committee participants will review the technical merits of the Grand Alliance proposal, which includes procedures for deciding on a few remaining component designs based on the results of specific tests. Various subgroups of the Advisory Committee will work with the Grand Alliance members as their merged system concept is finalized and, eventually, will oversee the testing of the completed system. Based on the results of those tests, the Committee may recommend the system to the FCC as the basis for a high definition television standard for our country. The FCC, of course, has the ultimate authority to adopt transmission standards. ENDS Footnote 1: MIT believes that a digital video broadcast standard that exclusively utilizes progressive scan transmission, from the beginning, is in the best interests of the United States. From huff@MCCLB0.MED.NYU.EDU Fri May 28 11:57:26 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA23443; Fri, 28 May 1993 15:56:20 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYPSEGBEQ80013CX@MCCLB0.MED.NYU.EDU>; Fri, 28 May 1993 16:57:28 EDT Date: Fri, 28 May 1993 16:57:26 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Move front image to end of newest compatible stack To: nih-image@soils.umn.edu Message-Id: <01GYPSEGN7420013CX@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT X-Sender: huff@mcclb0.med.nyu.edu (Unverified) You might find these macros useful (not exhaustively tested, but I don't think they contain any bugs :-). macro 'Make stack same size as front image'; var width, height: integer; name: string; begin SaveState; GetPicSize(width, height); SetNewSize(width, height); name := GetString('New Stack Name', 'stack'); MakeNewStack(name); RestoreState; end; macro 'Move front image to end of newest compatible stack'; var stkn, picn, width, height, w2, h2: integer; compat: Boolean; begin picn := PicNumber; if nSlices <> 0 then begin beep; exit; end; GetPicSize(width, height); stkn := nPics + 1 compat := false; while not compat do begin stkn := stkn - 1; if stkn = 0 then begin beep; exit; end; ChoosePic(stkn); GetPicSize(w2, h2); if width = w2 then if height = h2 then if nSlices <> 0 then if stkn <> picn then compat := true; end; ChoosePic(picn); SelectAll; Copy; ChoosePic(stkn); ChooseSlice(nSlices); AddSlice; Paste; ChoosePic(picn); Dispose; if picn < stkn then stkn := stkn - 1; SelectPic(stkn); SelectSlice(sliceNumber); end; -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From phskcc@emrycc.cc.emory.edu Fri May 31 08:33:00 1993 Received: from emoryu1.cc.emory.edu by nx1.soils.umn.edu (5.65c) id AA04607; Mon, 31 May 1993 12:41:17 -0500 Received: from emrycc.cc.emory.edu by emoryu1.cc.emory.edu (5.65/Emory_cc.3.4.6) via SMTP id AA11491 ; Mon, 31 May 93 13:42:32 -0400 Return-Path: phskcc@emrycc.cc.emory.edu Message-Id: <9305311742.AA11491@emoryu1.cc.emory.edu> Date: 31 May 93 13:33:00 EST From: "K. C. CHAN" Subject: RE: Plug-ins To: "nih-image" From: EMRYCC::WINS%"" 26-MAY-1993 12:49:40.64 To: PHSKCC CC: Subj: RE: Plug-ins >Is there a Plug-In for the HP Scanners? >A recent issue of MacWorld(or perhaps MacUser) had a review of scanners and >the HP Scanjet was the only scanner tested that did not come with a >Photoshop plug-in. >--wayne I just got a HP scanner HP SCAN IIc that comes with a Photoshop LE (limited edition) and a plug-in module called TWAIN. With TWAIN, users can use the Acquire command to acess the HP Scanner. The TWAIN plug-in module requires both the Photoshop LE and the device software--SOURCE MANAGER & DESCAN II-- provided by HP to work. Just like using Chooser from Apple menu, a TWAIN-supported device must be chosen first when the Acquire command is invoked from the File menu the first time. This can be done by choosing TWAINM Select Source from the Acquire submenu. I really feet sorry for the TWAIN Plug-in. It has many bugs and crashed numerous time on me. I don't know who should I point my finger to: HP, Adobe, or both? I wish the software can match up to the hardware. Wayne, can IMAGE supported a TWAIN-like plug-in? K.C. Chan Physics Dept. Emory University Atlanta GA 30322 (404)727-7863 PHSKCC@EMRYCC From jonker@amc.uva.nl Sat Jun 1 12:47:30 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA07463; Tue, 1 Jun 1993 04:47:06 -0500 Return-Path: Received: from HASARA5.BITNET (MAILGATE@HASARA5) by vx.cis.umn.edu (PMDF #2574 ) id <01GYUO5705OG9KM3B9@vx.cis.umn.edu>; Tue, 1 Jun 1993 04:49:23 CDT Received: from SARA.NL by SARA.NL for nih-image@soils.umn.edu; 1 Jun 93 11:48 MET Received: from amc.uva.nl by SARA.NL with PMDF#10201; Tue, 1 Jun 1993 11:47 MET Received: from [192.42.113.125] (Public_Mac_4.amc.uva.nl) by amc.uva.nl (PMDF #2498 ) id <01GYV2QDNLY80000G3@amc.uva.nl>; Tue, 1 Jun 1993 11:47:30 MET Date: 01 Jun 1993 11:47:30 +0100 (MET) Date-Warning: Date header was inserted by amc.uva.nl From: jonker@amc.uva.nl (Ard Jonker) Subject: Re: calcium ratioing and Image-VDM To: nih-image Message-Id: <01GYV2QKMCRA0000G3@amc.uva.nl> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT X-Sender: jonker@amccca.amc.uva.nl (Unverified) >We have just bought a Perceptics Pipeline board and are trying to start up >ratiometric Ca measurments using Image-VDM. This means acquiring two images, >subtracting the correpsonding background from each and then dividing one by the >other. The problem is that the program as it stands does not do division of >two images. Has anyone out there already adapted Image-VDM for such >operations or >had any experience with such applications? > >Any help would be much appreciated! Yes it does. If you first correct both images for dark current, then you copy one image and paste it into the other, with the 'paste control' window active, having 'divide' pressed once the images are aligned. We did feulgen/naphta yellow S stainging for nuclei and protein this way. Interactively, it is slow but it works fine with me. If you want to do this in another way, you might have a look at 'Imagine', another package for the mac. (don't use it without an FPU, or with softFPU, as it is __TERRIBLY_ slow; you'll think the machine crashed). you might also want to try MAIA (former MIRA, Macintosh Astronomical Image A(pplication?)) From sarmienu@rnisd0.DNET.roche.com Tue Jun 1 04:24:03 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA08006; Tue, 1 Jun 1993 07:24:12 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA02563; Tue, 1 Jun 93 08:24:04 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA08713; Tue, 1 Jun 93 08:22:51 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9306011222.AA08713@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Tue, 1 Jun 93 08:24:03 EDT Date: Tue, 1 Jun 93 08:24:03 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: Color Image I am intrigued about the use of 8 bit video in Image and why has color discrimination capabilities have all but ignored in the newest versions of Image. I would have thought that most people out there would be interested in such capability, even though discrimination by gray levels using the RGB channels is very effective... A couple of months back, I a discussion on Color-Image 1.32, a spin-off of image was talked about, however, it would not work on a Quadra 950. The author of that variant said he would post a new version of it. Did that ever happened? What about hardware support? apparently Scion-LG-3 can capture color but this spin-off does not support that board, Am I correct? Is it perhaps better to get IP-LAB for color analysis? Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 Sarmienu@RNISD0.DNET.ROCHE.COM From aljo@eel.sunet.se Tue Jun 1 17:36:45 1993 Received: from decnet.sunet.se by nx1.soils.umn.edu (5.65c) id AA08304; Tue, 1 Jun 1993 08:41:19 -0500 Return-Path: Received: from EEL.DECnet MAIL11D_V3 by decnet.sunet.se (5.57/Ultrix2.4-C) id AA17372; Tue, 1 Jun 93 15:36:46 +0200 Date: Tue, 1 Jun 93 15:36:45 +0200 Message-Id: <9306011336.AA17372@decnet.sunet.se> From: aljo@eel.sunet.se To: "nih-image@soils.umn.edu"@kth.sunet.se Subject: Highlighting Saturated pixels Is there a way for a user to select the colors which will be used to high- light pixels that are out of the exposure window? In the older versions, red was used to designate excessively dark regions and green identified the bright areas. With Image v1.49, the dark areas are now highlighted in blue which is extremely difficult to spot over a black region. It might be easier to use a light color. However, not all may agree so the best solution might be to make it possible for the user to select the colors. If anyone knows how to do this, would you please let me know. Thanks. Al Johnson aljo@eel.sunet.se Uppsala University Uppsala, Sweden From wayne@zippy.nimh.nih.gov Tue Jun 1 09:26:10 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA08615; Tue, 1 Jun 1993 09:26:52 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06206; Tue, 1 Jun 93 10:38:18 -0400 Message-Id: <9306011438.AA06206@zippy.nimh.nih.gov> Date: Tue, 1 Jun 1993 09:26:10 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: RE: Twain Plug-ins >I really feet sorry for the TWAIN Plug-in. It has many bugs and crashed numerous >time on me. I don't know who should I point my finger to: HP, Adobe, or both? >I wish the software can match up to the hardware. > >Wayne, can IMAGE supported a TWAIN-like plug-in? The Twain plug-in that comes with Photoshop 2.5 does not work with Image because Adobe refuses to allow their plug-ins to work with any program except Photoshop. Third party Twain plug-ins should work however, but right off hand, I don't know of any. --wayne From set@eru.mt.luth.se Tue Jun 1 18:01:08 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA08843; Tue, 1 Jun 1993 10:00:07 -0500 Return-Path: Received: from [130.240.1.173] (osse216.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA07531; Tue, 1 Jun 1993 17:01:08 +0200 Message-Id: <199306011501.AA07531@eru.mt.luth.se> Date: Tue, 1 Jun 1993 17:01:08 +0100 To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: Re: UserCode interface extension X-Content-Type: Text/Quoted-Readable >The timer support I posted to zippy.nimh.nih.gov in /pub/image/contrib >also had a change to the UserCode interface. I have thought about this, > >The proposal is: >(1) UserCode takes a variable number of arguments. I implemented this >without need to call putback token. > >(2) The maximum number is of arguments is five: > >UserCode(selector:integer;p1,p2,p3:real;str:string); > >(3) Selector value zero is defined to give the correct selector code >to use to call a named routine, i.e. > >Conv16To8 := UserCode(0,0,0,0,'Conv16To8'); > >Then later write UserCode(Conv16To8,p1,p2,p3); > >This also has the advantage that if a different version of Image is used, >probably the routine names will be different... Users would be prudent to >put a version check in so their macros check to see that the proper user.p >is included before trying to use usercode. > >(4) UserFunc(selector:integer,p1,p2,p3:real;str:string):real is also defined. >The same selector codes are used, in fact, the same code is executed. > >(5) UserStr(selector:integer,p1,p2,p3:real;str:string):string is also defined, >to permit user code to return a string. > >Send me any comments, or suggestions for further refinements in design. >This version has less runtime overhead than the previous design, and is >upward compatible with existing calls to UserCode. > >Also, from User.p, now you can pass strings back to MacroError. (Since >User.p comes before Macros in the build order, MacroError cannot be called >from within user.p. I fixed this by putting a var string argument to the >usermacrocode >procedure in user.p, which is set non-null to cause a macro error.) > >BTW I have a user.p which now supports 16 bit images and 32 bit real images, >with a few simple manipulations on them. Unfortunately many of the things >you want to do you can't, but it is possible to write macros that do some >simple processing using real arithmetic... And this is a framework for >easily incorporating other IP manipulations, although they can't be done >except via macros. >-- >Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 >Keck Laboratory for Biomolecular Imaging >NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 Dear Sir! We are generating 16-bits images from an AFM microscope. This images need to be matched with x- and y-offset, and thereafter subtracted. As i dont know the offset, I have to manually match them or do some form of 2DXcorr. I hope that this will give you some idea of our problem, and to help you with your spec. and code work. We are now waiting for a LC3-card with 32Mb memory, and will find some sollution fully use all the capacity of the framegrabber. We are planning to connect a ccd-camera to the card and grab 25 images/second for about 4 seconds. We will need some WORM-opto-disc's to. I do agree that we need some wayne-clones. It would also be helpfull to have a public rev. report on whats altered at new beta versions, or which modules are altered. ---- Best greetings from the arctic circuit ----- Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From wayne@zippy.nimh.nih.gov Tue Jun 1 10:26:45 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA09011; Tue, 1 Jun 1993 10:27:28 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06491; Tue, 1 Jun 93 11:38:53 -0400 Message-Id: <9306011538.AA06491@zippy.nimh.nih.gov> Date: Tue, 1 Jun 1993 10:26:45 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: RE: macro recording. >Macro recording is not terribly difficult, given that there is a >macro command for every menu item. To do it, we need a global variable >"MacroRecorder: Boolean" and a menu item "Start Recorder" which changes >to "Stop Recorder" when MacroRecorder is true. Then, for every menu >item chosen, in Image.p, add "if MacroRecorder then emitMacro('propercommand');" >or emitMacro('{}'); A macro recorder would probably not be very hard to implement once the text editor is done. All the macro recorder would have to do is a open a new text window when the user selects Start Recording and then to have each command in Image append the appropriate maco command to the text window. >Also, we need online help for writing macros. The online help package from >Disinfectant (available by FTP from John Nostrand) would be great. At the >moment, I usually keep microsoft word open to the macro appendix or copy >that into another text editor... Some user out there ought to put together >the text that would go into online help, basically a macro reference: list >of all functions in alphabetical order, and in the logical order... You will be able to use the new built-in text editor to open such lists since the it is is a multi-window editor. --wayne From wayne@zippy.nimh.nih.gov Tue Jun 1 10:32:13 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA09074; Tue, 1 Jun 1993 10:32:55 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA06505; Tue, 1 Jun 93 11:44:21 -0400 Message-Id: <9306011544.AA06505@zippy.nimh.nih.gov> Date: Tue, 1 Jun 1993 10:32:13 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: name of window in macro? >Now that the macro language has some string capabilities, would it be >possible to add a GetPicName() function? Several times I have thought >this would be useful: you could then name windows with processed images >after the parent image. For instance, a smoothing macro could take a >window called "Face", smooth it, and put the results in "Face Smoothed". I will look into adding such a function, but I will probably call it GetWindowName so it will also work with the text windows created by the new built-in text editor. --wayne From atm@finsandfur.fw.umn.edu Tue Jun 1 06:09:06 1993 Received: from mailbox.mail.umn.edu by nx1.soils.umn.edu (5.65c) id AA09359; Tue, 1 Jun 1993 11:07:23 -0500 Return-Path: Date: Tue, 1 Jun 93 11:09:06 CDT Message-Id: <9306011609.AA17197@mailbox.mail.umn.edu> Received: from x90-116-128.micro.umn.edu by mailbox.mail.umn.edu; Tue, 1 Jun 93 11:09:06 CDT From: "Alan McClure" To: nih-image@soils.umn.edu Subject: Frame Grabbers A colleague is considering the purchase of some image analysis equipment. I have been using Image for a while and I think he is sold on it. My question concerns what grabber board he should get. He needs to be able to capture images from video tape. I have been using a Perceptics board for capturing images from a camera and it seems to work but I gather from comments here that the Scion LG-3 board may be the preferred board. Does anyone have any comments or recommendations? Does anyone have a price, address, phone number, etc. Thanks for the help. Alan McClure Dept Fisheries and Wildlife University of Minnesota 200 Hodson Hall St. Paul MN 55108 e-mail atm@finsandfur.fw.umn.edu From csk@lm.dth.dk Tue Jun 1 20:37:34 1993 Received: from danpost.uni-c.dk by nx1.soils.umn.edu (5.65c) id AA09553; Tue, 1 Jun 1993 11:36:10 -0500 Return-Path: Received: from maaloe.lm.dth.dk by danpost.uni-c.dk (5.65c+/1.34) id AA21112; Tue, 1 Jun 1993 18:37:15 +0200 Received: by maaloe.lm.dth.dk (4.1/SMI-4.1) id AA01586; Tue, 1 Jun 93 18:37:34 +0200 Date: Tue, 1 Jun 93 18:37:34 +0200 From: csk@lm.dth.dk (Claus S. Kristensen) Message-Id: <9306011637.AA01586@maaloe.lm.dth.dk> To: nih-image@soils.umn.edu Subject: Digested mailing list Hi there, I get the Image mailing list, and as you know there is A LOT almost every day. This is good, of course, since that's what it is there for. However, I would like to suggest that subscribers optionally could subscribe to a digested list, with one daily posting, which then could include all the e-mails from the last 24 hours. (I guess the person to answer this should be the image-list administrators...) Please don't comment on this to the list, until an official reply has been posted (I wouldn't like to see my mailbox filled up with even more mail, just stating "I agree" or "Not for me, thanks!" (if I wasn't the one to put forward this proposal, that is). - Claus ------------------------------------------------------------------ * Claus Sternberg Kristensen * * Department of Microbiology Technical University of Denmark * * Building 221 DK 2800 Lyngby Denmark * * Phone (Den:) 45 93 12 22 ext. 2522 or 2515 * * Phone (Int:) +45 45 93 34 22 FAX +45 45 93 28 09 * ------------------------------------------------------------------ From ruzin@nature.berkeley.edu Tue Jun 1 03:12:15 1993 Received: from nettlerash.Berkeley.EDU by nx1.soils.umn.edu (5.65c) id AA09742; Tue, 1 Jun 1993 12:09:47 -0500 Return-Path: Received: from nature.Berkeley.EDU by nettlerash.berkeley.edu (5.67.1/1.34.6) id AA23979; Tue, 1 Jun 93 10:09:30 -0700 Received: from [128.32.128.165] (kos5mac10.Berkeley.EDU) by nature.berkeley.edu.cnr-net (4.1/SMI-4.1) id AA10779; Tue, 1 Jun 93 10:12:17 PDT Date: Tue, 1 Jun 93 10:12:15 PDT Message-Id: <9306011712.AA10779@nature.berkeley.edu.cnr-net> To: From: ruzin@nature.berkeley.edu (Steven Ruzin) Subject: Re: Frame Grabbers >A colleague is considering the purchase of some image analysis equipment. I >have been using Image for a while and I think he is sold on it. My question >concerns what grabber board he should get. He needs to be able to capture >images from video tape. I have been using a Perceptics board for capturing >images from a camera and it seems to work but I gather from comments here that >the Scion LG-3 board may be the preferred board. Does anyone have any >comments >or recommendations? Does anyone have a price, address, phone number, etc. >Thanks for the help. > >Alan McClure >Dept Fisheries and Wildlife >University of Minnesota >200 Hodson Hall >St. Paul MN 55108 >e-mail atm@finsandfur.fw.umn.edu Alan: The Scion board IS the best one. I also own the Data Translation board and don't use it. I much prefer the Scion. It's cheaper, comes with the cable, and Image supports it quite nicely. Scion LG-3 (Frederick MD) Frame grabber board $900 Scion Corporation 152 West Patrick Street Frederick, Maryland 21701 Phone: 301-695-7870 Fax: 301-695-0035 Dage-MTI CCD-72 B/W video camera $4000 Steve... Steven Ruzin NSF Center Dept Plant Biology Univ California Berkeley From John_Mansfield@mse.engin.umich.edu Tue Jun 1 12:33:14 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA09880; Tue, 1 Jun 1993 12:33:14 -0500 Return-Path: Message-Id: <199306011733.AA09880@nx1.soils.umn.edu> Date: 1 Jun 1993 13:33:28 U From: "John Mansfield" Subject: Re: Frame Grabbers To: nih-image@soils.umn.edu Reply_ RE>>Frame Grabbers Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu We have just purchased a RasterOps S24TV board to do colored capture from an optical microscope. Yes, I know the Scion will do color capture from an RGB camera (which is what we have), but We also want to record the images on to video tape and then grab some of the frames at a later date. This truns out to be tricky unless you invest in an RGB VCR and I dont know of such a beast. So, the camera has a composite (and SVHS) color output which we can record easily and the RasterOps board has the capability of capturing composite and SVHS video. Works nicely. One proble is that you cant use the Photoshop plug-in to record the image from the RaterOps board. The plug-in doesnt work unless you are in 24bit mode and Image only likes to run in 8 bit mode. If you try and switch color levels. in mid acquisition then you get crashes and unpredicatable results. Anyone else tried to use the RasterOps products with Image? BTW I too agree that the effort to clone Wayne should proceed as quickly as possible, I mean what if he should ever get sick?!! :-) Seriously Wayne, great job! Cheers Jfm. From wayne@zippy.nimh.nih.gov Tue Jun 1 13:20:10 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA10415; Tue, 1 Jun 1993 13:20:52 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA07154; Tue, 1 Jun 93 14:32:13 -0400 Message-Id: <9306011832.AA07154@zippy.nimh.nih.gov> Date: Tue, 1 Jun 1993 13:20:10 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: RasterOps Frame Grabbers >One proble is that you cant use the Photoshop plug-in to record the image from >the RaterOps board. The plug-in doesnt work unless you are in 24bit mode and >Image only likes to run in 8 bit mode. If you try and switch color levels. in >mid acquisition then you get crashes and unpredicatable results. Anyone else >tried to use the RasterOps products with Image? Are you sure your using the latest beta? I successfully tested the RasterOps 24XLTV frame grabber using the latest 1.50b52 beta. It fixes the problem that caused Image to crash if you switched to 24-bit and it also automatically switches back to 8-bit mode after the image has been acquired. --wayne From John_Mansfield@mse.engin.umich.edu Tue Jun 1 13:39:46 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA10570; Tue, 1 Jun 1993 13:39:46 -0500 Return-Path: Message-Id: <199306011839.AA10570@nx1.soils.umn.edu> Date: 1 Jun 1993 14:41:32 U From: "John Mansfield" Subject: Re: RasterOps Frame Grabber To: nih-image@soils.umn.edu Reply_ RE>>RasterOps Frame Grabbers Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu Maybe I want using the latest beta. Sorry for spreading gloomy news without foundation! I'll try the beta52 and see if it works. -------------------------------------- Date: 6/1/93 14:39 To: John Mansfield From: nih-image@soils.umn.edu >One proble is that you cant use the Photoshop plug-in to record the image from >the RaterOps board. The plug-in doesnt work unless you are in 24bit mode and >Image only likes to run in 8 bit mode. If you try and switch color levels. in >mid acquisition then you get crashes and unpredicatable results. Anyone else >tried to use the RasterOps products with Image? Are you sure your using the latest beta? I successfully tested the RasterOps 24XLTV frame grabber using the latest 1.50b52 beta. It fixes the problem that caused Image to crash if you switched to 24-bit and it also automatically switches back to 8-bit mode after the image has been acquired. --wayne ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;1 Jun 1993 14:39:00 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c0b9e67027727; Tue, 1 Jun 93 13:22:31 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA10438; Tue, 1 Jun 1993 13:21:03 -0500 Date: Tue, 1 Jun 1993 13:21:03 -0500 Message-Id: <9306011832.AA07154@zippy.nimh.nih.gov> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: wayne%zippy.nimh.nih.gov@relay.tc.umn.edu (Wayne Rasband) To: Multiple recipients of list Subject: Re: RasterOps Frame Grabbers From jamiel@sybase.com Tue Jun 1 14:47:22 1993 Received: from halon.sybase.com by nx1.soils.umn.edu (5.65c) id AA11369; Tue, 1 Jun 1993 14:47:22 -0500 Return-Path: Received: from sybase.com (sybgate.sybase.com) by halon.sybase.com (5.0/SMI-SVR4/SybFW4.0) id AA21360; Tue, 1 Jun 93 12:49:16 PDT Received: from ralph.sybgate.sybase.com by sybase.com (4.1/SMI-4.1/SybH3.2) id AA18799; Tue, 1 Jun 93 12:48:52 PDT Received: from Mac-mailer ([130.214.134.4]) by ralph.sybgate.sybase.com (4.1/SMI-4.1/SybEC3.1) id AA07463; Tue, 1 Jun 93 10:57:42 PDT Message-Id: <9306011757.AA07463@ralph.sybgate.sybase.com> Date: Tue, 01 Jun 93 10:58:41 From: jamiel@sybase.com (Jamie Lawrence) To: nih-image@soils.umn.edu Subject: artuses for image Content-Length: 505 Hi- I recieved several replys stating that indeed I am not the only one 'out there' not using image for research. Such being the case, I wanted to ask the other, much more substantial portion of this list if they would consider it a waste of thier bandwidth if the minority (artsie users, that is) talked other uses for image. If so, that is no problem for me- just wanted to check before I started throwing questions out and the like that others would get sick of seeing in thier inbox... jamie From SMASESM@UMSLVMA.UMSL.EDU Tue Jun 1 10:07:39 1993 Received: from umslvma.umsl.edu by nx1.soils.umn.edu (5.65c) id AA11653; Tue, 1 Jun 1993 15:11:00 -0500 Return-Path: Message-Id: <199306012011.AA11653@nx1.soils.umn.edu> Received: from UMSLVMA by UMSLVMA.UMSL.EDU (IBM VM SMTP V2R2) with BSMTP id 7820; Tue, 01 Jun 93 15:11:17 CDT Received: from UMSLVMA (SMASESM) by UMSLVMA (Mailer R2.08) with BSMTP id 5495; Tue, 01 Jun 93 15:11:17 CDT Date: Tue, 01 Jun 93 15:07:39 CDT From: "Mike Sesma, U.M.-St. Loui, School of Optometry" Subject: X-Y Stage encoders To: nih-image@soils.umn.edu Does anyone know of an X-Y stage encoder similar to the Minnesota Datametrics stage encoder and plotter/mapping software which can be interfaced with Image software. Minnesota datametrics has decided not to port the software to the Mac but will upgrade so that the encoder output can be fed through a serial channel. How would I go about interfacing such a unit. I am interested in cell mapping onto low mag sections from high mag viewing required for immuno- fluorescence. Thanks From pwf@unet.umn.edu Tue Jun 1 10:37:16 1993 Received: from unet.unet.umn.edu by nx1.soils.umn.edu (5.65c) id AA11950; Tue, 1 Jun 1993 15:36:03 -0500 Return-Path: Received: by unet.unet.umn.edu (5.65c) id AA13041; Tue, 1 Jun 1993 15:37:17 -0500 From: "Paul W. Fakler" Message-Id: <199306012037.AA13041@unet.unet.umn.edu> Subject: email test to nih-image@nx1.soils.umn.edu To: nih-image@soils.umn.edu Date: Tue, 1 Jun 1993 15:37:16 -0500 (CDT) X-Mailer: ELM [version 2.4 PL21] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 792 Just an email test to list nih-image@nx1.soils.umn.edu. Please ignore. I'm checking out a mail delivery problem. Paul. *-----------------------------------------------------------------------* | Paul W. Fakler | *---------------------------------*-------------------------------------* | Internet : pwf@unet.umn.edu | Networking Services | | Bitnet : pwf@umnacvx.bitnet | Computer and Information Services | | Desk : +1 612 626 1654 | University of Minnesota | | Problems : +1 612 625 0006 | 130 Lind Hall, 207 Church St SE | | FAX : +1 612 626 1002 | Minneapolis MN 55455-0134, USA | *---------------------------------*-------------------------------------* From jladwig@soils.umn.edu Tue Jun 1 11:08:05 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA12307; Tue, 1 Jun 1993 16:08:02 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA12540; Tue, 1 Jun 93 16:08:05 CDT Date: Tue, 1 Jun 93 16:08:05 CDT From: "John Ladwig" Message-Id: <9306012108.AA12540@saturn.soils.umn.edu> To: Subject: Re: artuses for image In-Reply-To: Jamie Lawrence's message <9306011757.AA07463@ralph.sybgate.sybase.com> of 1 June 1993 References: <9306011757.AA07463@ralph.sybgate.sybase.com> Jamie Lawrence writes on 1 June 1993 at 14:47:37 -0500 > [ ... ] I wanted to ask the other, much more substantial portion of > this list if they would consider it a waste of thier bandwidth if > the minority (artsie users, that is) talked other uses for image. If you're doing something with Image, I think it's right on topic here. This mailing list is not so busy that it needs a split, IMHO. -jml From mt1ntg@fencer.cis.dsto.gov.au Wed Jun 2 04:15:30 1993 Received: from fencer.cis.dsto.gov.au by nx1.soils.umn.edu (5.65c) id AA13274; Tue, 1 Jun 1993 18:14:29 -0500 Return-Path: Message-Id: <199306012314.AA13274@nx1.soils.umn.edu> Received: from fencer.cis.dsto.gov.au by fencer.cis.dsto.gov.au with SMTP; Wed, 2 Jun 1993 9:17:09 +1000 (EST) Date: Wed, 2 Jun 93 09:15:30 EST From: Noel Goldsmith Subject: Re: X-Y Stage encoders To: nih-image@soils.umn.edu X-Mailer: LeeMail 1.2.4 To Mike Sesma >Does anyone know of an X-Y stage encoder similar to the Minnesota Datametrics >stage encoder and plotter/mapping software which can be interfaced with Image >software. Minnesota datametrics has decided not to port the software to the >Mac but will upgrade so that the encoder output can be fed through a serial >channel. How would I go about interfacing such a unit. If you look at the directory user macros on zippy.nimh.nih.gov you will find a Macro which talks (successfully) to a Marzhauser Stage. This is a macro which sets up the necessary bytes or ascii characters as understandable words and then uses them via procedures in the macro language. Look at this. Serial devices can be a pain in the neck, but they usually work. If you want more help drop me a line. Regards Noel. I am interested in >cell mapping onto low mag sections from high mag viewing required for immuno- >fluorescence. Thanks > Aeronautical Research Laboratory 506 Lorimer St Port Melbourne Vic 3207 Australia Department of Defence Phone(03) 6477527 Fax (03) 6466771 Noel Goldsmith MT1NTG@Fencer.CIS.DSTO.GOV.AU From 00A0919@MSGATE.EMIS.hac.com Sat Jun 1 18:09:18 1993 Received: from hac2arpa.hac.com by nx1.soils.umn.edu (5.65c) id AA00878; Tue, 1 Jun 1993 20:11:33 -0500 Return-Path: <00A0919@MSGATE.EMIS.hac.com> Received: from GSGMVS.EMIS.HAC.COM ([192.27.11.14]) by hac2arpa.hac.com (4.1/SMI-DDN) id AA16365; Tue, 1 Jun 93 18:14:31 PDT Received: from MSGATE.EMIS.HAC.COM by GSGMVS.EMIS.HAC.COM (Soft-Switch Central V4L380P3); 01 Jun 1993 18:09:18 GMT Message-Id: Date: 01 Jun 1993 18:09:18 GMT From: "Sumida, David S" <00A0919@MSGATE.EMIS.hac.com> Subject: RE: Frame Grabbers To: nih-image@soils.umn.edu Comment: MEMO 06.01.93 18.09 ----- ITEM 1 ----- From: 00A0919@MRAM_EDEN@EDEN Author: Sumida, David S To: NIHIMAGE@SMTPGATE@SSW@EDEN Created: // Time: :: Subject: RE: Frame Grabbers To : eden::SSW::SMTPGATE::NIHIMAGE Date: 1-JUN-1993 16:31:38.00 >A colleague is considering the purchase of some image analysis equipment. I >have been using Image for a while and I think he is sold on it. My question >concerns what grabber board he should get. He needs to be able to capture >images from video tape. I have been using a Perceptics board for capturing >images from a camera and it seems to work but I gather from comments here that >the Scion LG-3 board may be the preferred board. Does anyone have any comments >or recommendations? Does anyone have a price, address, phone number, etc. >Thanks for the help. I see that someone has already passed on the Scion info for you. I just wanted to add that the nice feature about the Scion board is its expandable frame buffer which uses standard Mac SIMMS. Also, for your information and other's on the mailing list, I am running a resurrected Mac II using a Daystar Digital PowerCache Accelerator ('030 at 50 MHz) and IMAGE seems to be fully compatiable so far!! Blazing away, David Sumida, PhD Hughes Research Laboratories Malibu, CA 90265 00A0919@msgate.emis.hac.com _______________________________________________________________________________ From: NIHIMAGE on Tue, Jun 1, 1993 10:11 AM Subject: Frame Grabbers To: Sumida, David S Received: from EDEN1.HAC.COM by GSGMVS.EMIS.HAC.COM (Soft-Switch Central V4L380P3); 01 Jun 1993 09:11:09 GMT Received: from mail.tc.umn.edu (128.101.131.1) by EDEN1.HAC.COM (PMDF #2669 ) id <01GYUXAC7D8G005G1X@EDEN1.HAC.COM>; Tue, 1 Jun 1993 09:11:38 PST Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c0b7f95025982; Tue, 1 Jun 93 11:11:01 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA09406; Tue, 1 Jun 1993 11:09:41 -0500 Date: 01 Jun 1993 11:09:41 -0500 From: Alan McClure Subject: Frame Grabbers Sender: nih-image@soils.umn.edu To: Multiple recipients of list Errors-to: owner-nih-image@soils.umn.edu Reply-to: nih-image@soils.umn.edu Message-id: <9306011609.AA17197@mailbox.mail.umn.edu> X-Envelope-to: 00a0919@msgate.emis.hac.com Content-transfer-encoding: 7BIT Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas A colleague is considering the purchase of some image analysis equipment. I have been using Image for a while and I think he is sold on it. My question concerns what grabber board he should get. He needs to be able to capture images from video tape. I have been using a Perceptics board for capturing images from a camera and it seems to work but I gather from comments here that the Scion LG-3 board may be the preferred board. Does anyone have any comments or recommendations? Does anyone have a price, address, phone number, etc. Thanks for the help. Alan McClure Dept Fisheries and Wildlife University of Minnesota 200 Hodson Hall St. Paul MN 55108 e-mail atm@finsandfur.fw.umn.edu From SBK111@SBMS01.MSRC.SUNYSB.EDU Tue Jun 1 17:20:00 1993 Received: from sbms01.msrc.sunysb.edu by nx1.soils.umn.edu (5.65c) id AA00900; Tue, 1 Jun 1993 20:17:57 -0500 Return-Path: Date: Tue, 1 Jun 93 21:20 EDT From: Barr Kum Subject: Re: Color and temperature To: nih-image@soils.umn.Edu Message-Id: <29B4E714247F806335@SBMS01.MSRC.SUNYSB.EDU> X-Organization: Marine Sciences Research Center X-Envelope-To: nih-image@soils.umn.Edu X-Vms-To: IN%"nih-image@soils.umn.Edu" X-Vms-Cc: SBK111 Here are a few references relationg Temperature and radiation Badenas, C and V. Caselles 1992. A simple technique for estimating surface temperature by means of a thermal infrared radiometer. Int. J. of Remote Sensing vol 13 no. 15 2951-2956. Cracknell, A. P. 1992 A method for the correction of sea surface temperatures derived from satellite thermal infrared data in an area of sunglint. Int. J. of Remote Sensing, 1993 vol 14 no. 1 3-8 McClain, E. P.., Pichel, G. W. and Walton C. C. 1985. Comparative performance of AVHRR-based, multichannel Sea Surface temperature. Journal of Geophysical Research, 90C, 11, 587-11, 601. Hope this helps Barr-Kum sbarrkum@csserv1.ic.sunysb.edu sbk111@msrc.sunysb.edu From contper1@NKI.NL Sun Jun 2 10:04:00 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA03115; Wed, 2 Jun 1993 02:01:31 -0500 Return-Path: Received: from HASARA5.BITNET (MAILGATE@HASARA5) by vx.cis.umn.edu (PMDF #2574 ) id <01GYVWPCV6VK9KM5PA@vx.cis.umn.edu>; Wed, 2 Jun 1993 02:05:29 CDT Received: from SARA.NL by SARA.NL for nih-image@nx1.soils.umn.edu; 2 Jun 93 9:04 MET Received: from NKI.NL by SARA.NL with PMDF#10201; Wed, 2 Jun 1993 09:04 MET Received: from [192.42.114.110] by NKI.NL; Wed, 2 Jun 93 09:03 GMT Date: 02 Jun 1993 09:04 +0100 (MET) From: contper1@NKI.NL Subject: problem with grabbing To: nih-image@soils.umn.edu Message-Id: <2952BAEF683F002DDB@NKI.NL> X-Envelope-To: nih-image@nx1.soils.umn.edu Content-Transfer-Encoding: 7BIT Date-Note: Date: header was inserted by SARA.NL X-Sender: contper1@nki.nl (Unverified) X-Attachments: :Data:99:QckCapInit: ------------------------------------------------------------------------------ ilja van de pavert antoni van leeuwenhoekhuis/the netherlands cancer institute dept. of biophysics plesmanlaan 121 phone: +31 20 5121894 1066 cx amsterdam fax: +31 20 5121944 the netherlands email: contper1@nki.nl ---------------------------------------------------------------------------- -- I'am using Image 1.49 with the DataTranslation framegrabber card DT2255 and QckCapInit 01.04. Grabbing can be done, but the application hangs as soon as I stop grabbing. I got last week a Quadra800 with 24 MB RAM and checked all possibilities: (nearly) all inits off and cache off, but the computer still hangs. Image 1.50b52 has the same behaviour. My previous machine was a MacII (68020, 8MB RAM) with QckCapInit 01.01. Any suggestions how to overcome this problem? From @cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be Thu Jun 3 04:01:24 1993 Received: from cc1.kuleuven.ac.be by nx1.soils.umn.edu (5.65c) id AA12863; Thu, 3 Jun 1993 04:01:24 -0500 Return-Path: <@cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be> Received: from rc1.vub.ac.be by cc1.kuleuven.ac.be (IBM VM SMTP V2R2) with TCP; Thu, 03 Jun 93 11:04:54 +0200 Received: from dec5.ulb.ac.be (dbmdec5) by rc1.vub.ac.be (4.1/RC1-930416) id AA22395; Thu, 3 Jun 93 11:04:23 +0200 Received: by dec5.ulb.ac.be (5.65/ULB.920908) id AA20407; Thu, 3 Jun 1993 11:01:16 +0100 From: jvanheld@dbm.ulb.ac.be (Jacques VAN HELDEN) Message-Id: <9306031001.AA20407@dec5.ulb.ac.be> Subject: light integration with CCD camera To: NIH-IMAGE@soils.umn.edu Date: Thu, 3 Jun 93 11:01:15 WET DST Cc: jvanheld@dbm.ulb.ac.be X-Mailer: ELM [version 2.3 PL11] Some video cameras allow to integrate the light falling on the CCD chip for several seconds to minutes (VideoScope, Cohu, ...). Does anyone use this functionnality with NIH image ? * Jacques van Helden * * Universite Libre de Bruxelles * * Service de Neurobiologie * * 67 rue des Chevaux * * B-1640 Rhodes Saint Genese * * Belgium * * tel: (32) 2 650 99 41 * * fax: (32) 2 650 97 44 * * e-mail: jvanheld@ulb.ac.be * From huff@MCCLB0.MED.NYU.EDU Thu Jun 3 06:13:14 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA14437; Thu, 3 Jun 1993 10:10:25 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GYXU4SC6GW001GRX@MCCLB0.MED.NYU.EDU>; Thu, 3 Jun 1993 11:13:16 EDT Date: Thu, 03 Jun 1993 11:13:14 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Image 1.44 Markup source released To: nih-image@soils.umn.edu Message-Id: <01GYXU4SNELE001GRX@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT Since a few people have asked for it, I have posted the source code for the graphic overlay "markup" window to zippy.nimh.nih.gov in /pub/image/contrib. I also posted a copy of the application and a large macro file which we used with it. The previously posted application did not include a macro file and lacks one minor change. The source code file includes MPW compare output to show the changes from released 1.44, including changes to image.rsrc. I used Stuffit Classic to create Stuffit 1.4.1 archives, and binhexed them before transfer. BTW this source code also includes unlimited macro source size and a fix for the "else if" vs. "else begin if" feature of the macro interpreter. Use this program if you are thinking of tracing things on a piece of acetate held up to the screen and then putting the acetate on a scanner. One of these days I hope to get a manual of sorts together. Also, maybe parts of this will make it into mainline image. Some things we just don't have software to recognize, and the human eye is required in the image processing loop. This program is intended for such situations. To use in simple mode, just open an image and choose "show markup" From the analyze menu. When the markup window is front, the pencil tool Received: from VILL.EDU (wild.ucis.vill.edu) by nx1.soils.umn.edu (5.65c) id AA17313; Thu, 3 Jun 1993 19:03:54 -0500 Resent-From: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Message-Id: <199306040003.AA17313@nx1.soils.umn.edu> Return-Path: @VILL.EDU:mobasser@vu-vlsi.ee.vill.edu Resent-Date: Thu, 3 Jun 1993 20:11 EST Received: from vu-vlsi.ee.vill.edu [153.104.63.20] by VILL.EDU with SMTP-VMS via TCP/IP; Thu, 3 Jun 1993 20:11 EST Received: by vu-vlsi.ee.vill.edu (4.1/SMI-4.1a) id AA25437; Thu, 3 Jun 93 20:08:22 EDT From: mobasser@vu-vlsi.ee.vill.edu (Bijan Mobasseri) Message-Id: <9306040008.AA25437@vu-vlsi.ee.vill.edu> Subject: Re: Color and temperature To: nih-image@soils.umn.edu Date: Thu, 3 Jun 1993 20:08:21 -0400 (EDT) In-Reply-To: <29B4E714247F806335@SBMS01.MSRC.SUNYSB.EDU> from "Barr Kum" at Jun 3, 93 04:58:03 pm X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 14 Thanks a lot From Carl_Gustafson@cbis.ece.drexel.edu Thu Jun 3 19:36:36 1993 Received: from cbis.ece.drexel.edu by nx1.soils.umn.edu (5.65c) id AA17544; Thu, 3 Jun 1993 19:36:36 -0500 Return-Path: Received: from homer.ece.drexel.edu by cbis.ECE.Drexel.EDU (5.67/1.34) id AA07052; Thu, 3 Jun 93 20:39:31 EDT Message-Id: <9306040039.AA07052@cbis.ECE.Drexel.EDU> Date: 3 Jun 1993 20:39:50 U From: "Carl Gustafson" Subject: Re: problem with grabbing To: nih-image@soils.umn.edu Reply to: RE>problem with grabbing >I'am using Image 1.49 with the DataTranslation framegrabber card DT2255 and >QckCapInit 01.04. >Grabbing can be done, but the application hangs as soon as I stop grabbing. >I got last week a Quadra800 with 24 MB RAM and checked all possibilities: >(nearly) all inits off and cache off, but the computer still hangs. Image >1.50b52 has the same behaviour. My previous machine was a MacII (68020, 8MB >RAM) with QckCapInit 01.01. >Any suggestions how to overcome this problem? Try turning the cache off, using the cache control control panel (I forget the exact name, but it comes with the system software.) Carl Gustafson From rjb1@cec2.wustl.edu Thu Jun 3 17:14:24 1993 Received: from cec2.wustl.edu by nx1.soils.umn.edu (5.65c) id AA18187; Thu, 3 Jun 1993 22:11:24 -0500 Return-Path: Received: by cec2.wustl.edu (5.65a/1.35); id AA11121; Thu, 3 Jun 93 22:14:24 -0500 Date: Thu, 3 Jun 93 22:14:24 -0500 From: rjb1@cec2.wustl.edu (Randall John Bateman) Message-Id: <9306040314.AA11121@cec2.wustl.edu> To: nih-image@soils.umn.edu Subject: unsubscribe Randall Bateman unsubscribe Randall Bateman unsubscribe rjb1@cec2.wustl.edu From aljo@eel.sunet.se Fri Jun 4 10:14:27 1993 Received: from decnet.sunet.se by nx1.soils.umn.edu (5.65c) id AA19014; Fri, 4 Jun 1993 01:11:54 -0500 Return-Path: Received: from EEL.DECnet MAIL11D_V3 by decnet.sunet.se (5.57/Ultrix2.4-C) id AA26609; Fri, 4 Jun 93 08:14:27 +0200 Date: Fri, 4 Jun 93 08:14:27 +0200 Message-Id: <9306040614.AA26609@decnet.sunet.se> From: aljo@eel.sunet.se To: "nih-image@soils.umn.edu"@kth.sunet.se Subject: Re: problem with grabbing DO YOU REALLY NED TO USE THE QUICK CAPTURE INIT? From mvivino@helix.nih.gov Fri Jun 4 04:18:59 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA20460; Fri, 4 Jun 1993 07:16:02 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA03840; Fri, 4 Jun 93 08:18:59 -0400 Date: Fri, 4 Jun 93 08:18:59 -0400 Message-Id: <9306041218.AA03840@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: light integration with CCD camera >Some video cameras allow to integrate the light falling on the CCD >chip for several seconds to minutes (VideoScope, Cohu, ...). > >Does anyone use this functionnality with NIH image ? I have tried using the cohu integrating camera and Image and a data translation card. The camera can act in normal 30 fps mode or in integrating mode. For integrating you hold a line down till you are done the desired integration, then a pulse returns on another line to tell your frame grabber that the integrated field is on the way. The return is only a field and not a whole frame so you need to remove the odd or even lines. I like the cohu although I don't feel that the combination consistently captured what I wanted. I'm not sure if the triggering mechanism is incredibly smooth. Mark NIH/DCRT From mvivino@helix.nih.gov Fri Jun 4 04:22:06 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA20499; Fri, 4 Jun 1993 07:19:13 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA03984; Fri, 4 Jun 93 08:22:06 -0400 Date: Fri, 4 Jun 93 08:22:06 -0400 Message-Id: <9306041222.AA03984@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: problem with grabbing My previous machine was a MacII (68020, 8MB >RAM) with QckCapInit 01.01. >Any suggestions how to overcome this problem? You do not need the init, take it out of your system folder. If you still crash, then perhaps you are having other problems. MAXA software can find bad memory addresses, Alert can help find conflicting inits, and norton or mactools can see that your hardisk is ok. there is also rebuilding the desktop. Mark Vivino NIH/DCRT From John_Mansfield@mse.engin.umich.edu Fri Jun 4 10:02:44 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA21361; Fri, 4 Jun 1993 10:02:44 -0500 Return-Path: Message-Id: <199306041502.AA21361@nx1.soils.umn.edu> Date: 4 Jun 1993 11:04:01 U From: "John Mansfield" Subject: Re: light integration with To: nih-image@soils.umn.edu Reply_ RE>>light integration with C Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu I have a colleague who uses an integrating Fairchild camera with his software and hardware package that is designed for the analysis of LEED and RHEED data. He uses a Data Translation frame grabber in a PC and controls the camera from a serial line. The integration occurs as follows. You watch the frame sync signal from the camera and when it has finished sending a video frame you set the output inhbit control line high by setting 5 volts on the serial line connection from the PC. You wait for x /130ths of a second and then set the inhibit line low and on the next frame grab the image into the frame grabber. During the integrate, the camera is still generating the video signals, it just isnt sending a picture. This gives you only one lot of readout noise. If you use the Scion LG-3 with with say 16meg of memory you can digitize up to 32 consecutive video frames and average those, but in this case you get 32 times the readout noise as compared with integrating on the CCD for 32/30ths of a second (True, you may saturate the chip in this example, but that is what it is, just an example). Now, it turns out that the SCion LG-3 board is capable of doing exactly what my colleague does with the PC system. You can use the control lines of the LG-3 to set the inhibit line on the CCD. The tricky bit is watching the video from signals and making sure that you dont try and integrate 1/2 way through a frame. I havent written any code to do this integration yet, we are not sure if it will seriously improve our images. I think we will have to write special code for this, I dont think the macro interpreter will suffice. Just for info, the guy with the PC system for LEED and RHEED analysis is: Darryl Bartlett k-Space Associated Inc. Ann Arbor Michigan Phone and FAX: 313-761-9315 Just my musings! Jfm. -------------------------------------- Date: 6/4/93 8:38 To: John Mansfield From: nih-image@soils.umn.edu >Some video cameras allow to integrate the light falling on the CCD >chip for several seconds to minutes (VideoScope, Cohu, ...). > >Does anyone use this functionnality with NIH image ? I have tried using the cohu integrating camera and Image and a data translation card. The camera can act in normal 30 fps mode or in integrating mode. For integrating you hold a line down till you are done the desired integration, then a pulse returns on another line to tell your frame grabber that the integrated field is on the way. The return is only a field and not a whole frame so you need to remove the odd or even lines. I like the cohu although I don't feel that the combination consistently captured what I wanted. I'm not sure if the triggering mechanism is incredibly smooth. Mark NIH/DCRT ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;4 Jun 1993 08:37:50 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c0f3dce026109; Fri, 4 Jun 93 07:19:27 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA20483; Fri, 4 Jun 1993 07:16:14 -0500 Date: Fri, 4 Jun 1993 07:16:14 -0500 Message-Id: <9306041218.AA03840@helix.nih.gov> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: mvivino%helix.nih.gov@relay.tc.umn.edu To: Multiple recipients of list Subject: Re: light integration with CCD camera From wayne@zippy.nimh.nih.gov Fri Jun 4 10:57:39 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA21755; Fri, 4 Jun 1993 10:56:38 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA15380; Fri, 4 Jun 93 12:09:52 -0400 Message-Id: <9306041609.AA15380@zippy.nimh.nih.gov> Date: Fri, 4 Jun 1993 10:57:39 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: light integration with CCD camera >Some video cameras allow to integrate the light falling on the CCD >chip for several seconds to minutes (VideoScope, Cohu, ...). > >Does anyone use this functionnality with NIH image ? Paultek Imaging(916-265-9293, fax: 916-265-0587) has a full frame integrating CCD camera with RS-232 control that looks like it would work well with NIH Image. --wayne From geller@synapse.umdnj.edu Fri Jun 4 05:39:15 1993 Received: from synapse.UMDNJ.EDU by nx1.soils.umn.edu (5.65c) id AA22714; Fri, 4 Jun 1993 13:14:17 -0500 Return-Path: Received: by synapse.umdnj.edu id aa05998; 4 Jun 93 9:51 EDT Date: Fri, 4 Jun 93 9:39:15 EDT From: "Herbert M. Geller" To: nih-image@soils.umn.edu Subject: Re: light integration with CCD camera Message-Id: <9306040939.aa05983@synapse.umdnj.edu> I just bought a Dage C72 CCD camera that can integrate, and I intend to use it with NIH Image. However, the equipment is not here yet, so I haven't had any experience. Herb Geller From chien@jeeves.UCSD.EDU Fri Jun 4 04:36:43 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA22907; Fri, 4 Jun 1993 13:33:48 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA09398 sendmail 5.67/UCSD-2.2-sun via SMTP Fri, 4 Jun 93 11:36:45 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA25244 to nih-image@soils.umn.edu; Fri, 4 Jun 93 11:36:43 PDT Date: Fri, 4 Jun 93 11:36:43 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306041836.AA25244@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: noise during integration John Mansfield mentions: > ...If you > use the Scion LG-3 with with say 16meg of memory you can digitize up to 32 > consecutive video frames and average those, but in this case you get 32 times > the readout noise as compared with integrating on the CCD for 32/30ths of a > second. This is not quite right. Assuming that the readout noise is random and there- fore incoherent (as opposed to a fixed pattern noise that repeats from frame to frame), the noise in an average of 32 frames would only be sqrt(32)=5.7 times the noise in a single frame. That's why averaging helps: your signal- to-noise ratio goes up as sqrt(N). Pedantically, Chi-Bin Chien From scudder@cpac.washington.edu Fri Jun 4 04:44:29 1993 Received: from bailey.cpac.washington.edu by nx1.soils.umn.edu (5.65c) id AA23041; Fri, 4 Jun 1993 13:43:07 -0500 Return-Path: Received: by bailey.cpac.washington.edu (5.65/UW-NDC Revision: 2.4 ) id AA08845; Fri, 4 Jun 93 11:45:41 -0700 Date: Fri, 4 Jun 1993 11:44:29 -0700 (PDT) From: Kurt Scudder Sender: Kurt Scudder Reply-To: Kurt Scudder Subject: Re: light integration with CCD camera To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <9306031001.AA20407@dec5.ulb.ac.be> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII We are using just such an arrangement in our lab. The COHU 4900 series cameras will integrate on-chip. There is an auxiliary connector on the camera control box (it's a remote head camera). To integrate, you simply apply a logic low to one of the control lines. The readout of the CCD is prevented while the control line is held low. When the control line is pulled back high, the frame of video is read out on the next sync pulse. The camera controller generates a "grab pulse" at the instant the integrate line goes high. We feed this into the external trigger on the Data Translation QuickCapture board, and just enable the "Wait for Trigger" mode in Image. It is slick and simple - the hardest part about it was hand-building the cable to connect it all together. The integration signal comes from our UniBlitz shutter controller. As soon as the shutter opens, the camera starts to integrate. When the shutter closes, the camera stops integrating and the integrated frame is captured by Image. The Uniblitz shutter controller is an exceptionally nice piece of equipment. It has built-in timers (4-digit), or it can be computer-controlled. You can fire the internal timers by computer, or you can override them such that the computer controls the duration of the shutter/integration. It can be manually opened or closed, set to oscillate with defined open/closed periods, or used as a one-shot mode either normally open or normally closed. It is far more versatile than we will ever need, and was only about US$350 We are currently using another computer to fire the shutter controller, or are triggering the shutter manually. With a little ingenuity, I think the serial port could be used from within Image to do the triggering. The beauty of using the Uniblitz shutter controller is that it essentially becomes the data acquisition timer if you are capturing a series of images. The computer doesn't have to do any timing. There are two drawbacks. First, it is next to impossible to follow the video on an external monitor, because the integrated video frame only comes out once at the end of the integration period. What you see is a blank screen, then a brief (1/30 sec, of course, in the USA) image, then a blank screen again until the next frame. Of course the captured image is displayed continuously on the mac screen. Second, although COHU says you can integrate for several seconds before the noise kills you, we have found that after one second, there are tens to hundreds of saturated pixels scattered randomly in the image even with no light falling on the camera. It is hard to correct for this, but one could write a macro to ignore those pixels. They're the same every time. Kurt Scudder Dept. of Chemistry University of Washington Seattle, WA USA scudder@u.washington.edu On Thu, 3 Jun 1993, Jacques VAN HELDEN wrote: > Some video cameras allow to integrate the light falling on the CCD > chip for several seconds to minutes (VideoScope, Cohu, ...). > > Does anyone use this functionnality with NIH image ? > > > * Jacques van Helden * > * Universite Libre de Bruxelles * > * Service de Neurobiologie * > * 67 rue des Chevaux * > * B-1640 Rhodes Saint Genese * > * Belgium * > * tel: (32) 2 650 99 41 * > * fax: (32) 2 650 97 44 * > * e-mail: jvanheld@ulb.ac.be * > > From John_Mansfield@mse.engin.umich.edu Fri Jun 4 14:11:51 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA23314; Fri, 4 Jun 1993 14:11:51 -0500 Return-Path: Message-Id: <199306041911.AA23314@nx1.soils.umn.edu> Date: 4 Jun 1993 14:51:49 U From: "John Mansfield" Subject: Re: noise during integration To: nih-image@soils.umn.edu Reply_ RE>noise during integration Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu Yeah you are right, but it's still much worse right? That's why you would like to do the on chip integration! :-) Cheers! Jfm. -------------------------------------- Date: 6/4/93 14:38 To: John Mansfield From: nih-image@soils.umn.edu John Mansfield mentions: > ...If you > use the Scion LG-3 with with say 16meg of memory you can digitize up to 32 > consecutive video frames and average those, but in this case you get 32 times > the readout noise as compared with integrating on the CCD for 32/30ths of a > second. This is not quite right. Assuming that the readout noise is random and there- fore incoherent (as opposed to a fixed pattern noise that repeats from frame to frame), the noise in an average of 32 frames would only be sqrt(32)=5.7 times the noise in a single frame. That's why averaging helps: your signal- to-noise ratio goes up as sqrt(N). Pedantically, Chi-Bin Chien ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;4 Jun 1993 14:38:44 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c0f965a000542; Fri, 4 Jun 93 13:37:15 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA22930; Fri, 4 Jun 1993 13:33:57 -0500 Date: Fri, 4 Jun 1993 13:33:57 -0500 Message-Id: <9306041836.AA25244@jeeves.UCSD.EDU> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: chien%jeeves.UCSD.EDU@relay.tc.umn.edu (Chi-Bin Chien) To: Multiple recipients of list Subject: noise during integration From @cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be Mon Jun 7 06:18:49 1993 Received: from cc1.kuleuven.ac.be by nx1.soils.umn.edu (5.65c) id AA09414; Mon, 7 Jun 1993 06:18:49 -0500 Return-Path: <@cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be> Received: from rc1.vub.ac.be by cc1.kuleuven.ac.be (IBM VM SMTP V2R2) with TCP; Mon, 07 Jun 93 13:21:59 +0200 Received: from dec5.ulb.ac.be (dbmdec5) by rc1.vub.ac.be (4.1/RC1-930416) id AA20433; Mon, 7 Jun 93 13:22:03 +0200 Received: by dec5.ulb.ac.be (5.65/ULB.920908) id AA20160; Mon, 7 Jun 1993 13:18:48 +0100 From: jvanheld@dbm.ulb.ac.be (Jacques VAN HELDEN) Message-Id: <9306071218.AA20160@dec5.ulb.ac.be> Subject: Re: noise during integration To: NIH-IMAGE@soils.umn.edu Date: Mon, 7 Jun 93 13:18:47 WET DST X-Mailer: ELM [version 2.3 PL11] > Second, although COHU says you can integrate for several seconds > before the noise kills you, we have found that after one second, there > are tens to hundreds of saturated pixels scattered randomly in the > image even with no light falling on the camera. It is hard to correct > for this, but one could write a macro to ignore those pixels. They're > the same every time. Using the VideoScope CCD200E camera, I have the same effect with long integration. This noise (which is not really random since always on the same positions) can be removed with the Reduce Noise function of NIH-Image, which makes a median filtering (each point is replaced by the median value of the pixels in a 3x3 square around itself). You loose a bit resolution, but it is an edge preserving filtering. Jacques van Helden jvanheld@dbm.ulb.ac.be From chien@jeeves.UCSD.EDU Mon Jun 7 06:39:59 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA15792; Mon, 7 Jun 1993 15:37:11 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA20541 sendmail 5.67/UCSD-2.2-sun via SMTP Mon, 7 Jun 93 13:40:01 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA06270 to nih-image@soils.umn.edu; Mon, 7 Jun 93 13:39:59 PDT Date: Mon, 7 Jun 93 13:39:59 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306072039.AA06270@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: bad pixels, long integration > Second, although COHU says you can integrate for several seconds > before the noise kills you, we have found that after one second, there > are tens to hundreds of saturated pixels scattered randomly in the > image even with no light falling on the camera. It is hard to correct > for this, but one could write a macro to ignore those pixels. They're > the same every time. > Using the VideoScope CCD200E camera, I have the same effect with long > integration. This noise (which is not really random since always on the > same positions) can be removed with the Reduce Noise function of > NIH-Image, which makes a median filtering. I believe that this effect is common among cameras using consumer-grade CCD chips: some of the CCD's pixels are bad (probably high dark current). Using Reduce Noise seems like a good way to get rid of them if you don't mind blurring your picture a little bit... For those whose cameras do not integrate, one could use a digital video integrator such as the ones from Dage. Their first box (about $4000) just let you do simple integrations, and worked quite nicely when I demoed it. I believe they have a second-generation box out (about $6000), though I haven't seen it in action, which sounds like a really nice machine. It has several planes of memory, so it can do background subtraction etc., has an additional 1-bit plane which is meant for masking out bad pixels (exactly the application mentioned above). I also seem to remember that it was supposed to have a digital data interface (SCSI?), with Mac drivers. Not having seen this box in the flesh, I can't swear to the details, but it should be a worthy competitor to the Hamamatsu Argus-10. It would be useful for Image users who need to do processing at higher speeds than their Macs can handle, or for storing large amounts of data to videotape. Chi-Bin Chien From stmwang@hubcap.clemson.edu Mon Jun 7 17:54:19 1993 Received: from hubcap.clemson.edu by nx1.soils.umn.edu (5.65c) id AA18171; Mon, 7 Jun 1993 20:51:38 -0500 Return-Path: Received: by hubcap.clemson.edu; Mon, 7 Jun 93 21:54:20 -0400 From: stmwang@hubcap.clemson.edu (Sam Wang) Message-Id: <9306080154.AA08754@hubcap.clemson.edu> Subject: JPEG available for Image? To: nih-image@soils.umn.edu Date: Mon, 7 Jun 1993 21:54:19 -0400 (EDT) In-Reply-To: <9306071218.AA20160@dec5.ulb.ac.be> from "Jacques VAN HELDEN" at Jun 7, 93 06:19:02 am X-Mailer: ELM [version 2.4 PL2] Content-Type: text Content-Length: 381 Please excuse me if this has been answered before: are there ways Image can save/read files compressed in JPEG format? I know the PhotoShop JPEG plugin doesn't work with the 1.5beta (I tried), but are there third party plug-ins that might? Thanks. Sam -- Sam Wang stmwang@hubcap.clemson.edu 803/656-3924voice 656-0204fax Art Dept., Clemson University, Clemson, SC 29634-0509 From chien@jeeves.UCSD.EDU Mon Jun 7 16:15:51 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA19312; Tue, 8 Jun 1993 01:13:02 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA09283 sendmail 5.67/UCSD-2.2-sun via SMTP Mon, 7 Jun 93 23:15:53 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA14259 to nih-image@soils.umn.edu; Mon, 7 Jun 93 23:15:51 PDT Date: Mon, 7 Jun 93 23:15:51 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306080615.AA14259@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: Trouble grabbing from VCR with Scion I am forwarding this query from Eric Olson, a graduate student in another lab in my department: =============== Re: Image importing failures into the Scion LG-3 that are dependent on VCR tape quality. We purchased a Scion LG-3 frame grabber (5/93) which imports images nicely From a SIT camera but sporadically fails to import images from VCR. This f Received: from esseX.StFX.CA by nx1.soils.umn.edu (5.65c) id AA20731; Tue, 8 Jun 1993 09:30:57 -0500 Return-Path: Received: by esseX.stfx.ca (MX V2.3) id 12283; Tue, 08 Jun 1993 10:57:19 AST Date: Tue, 08 Jun 1993 10:57:19 AST From: John Corbit To: nih-image@soils.umn.edu Cc: garbary@esseX.stfx.ca Message-Id: <0096DB74.25DE18C0.12283@esseX.stfx.ca> Subject: grayscale vs halftoning MX%"nih-image@soils.umn.edu" I have a new (05/93) Scion LG-3 board (S/N 30133) installed in my Quadra 800 and have begun to experiment with image capture using Image 1.50b52 and the LG-3 plug-in. I have a JVC TK-885U CCD camera on approval to try with the board. My question concerns whether this is the wrong camera for grayscale photography. I am getting images but they are halftoned, both on the screen and when printed by inkjet or laserprinter. When printed from PhotoShop to a laser printer the images are dithered. My expectation was that there would be no half-toning or dithering, and that each pixel would be one of 256 grays. This is not occurring. Presumably the problem comes from the camera. First of all it is a color camera with the following items noted in the list of specs: color video camera; single CCD complimentary color system; no. of effective pixels 510(H) x 492(V); scanning lines 525, 2:1 interlaced; horizontal resolution 330 TV lines. Is this camera no good for gray-scale photography? Is there a problem because 510x492 **EFFECTIVE PIXELS** does not match the 640x480 pixels of the board? What are "effective pixels"? I will be very grateful to hear from you about whether I am doing something wrong or whether this camera is no good for gray-scale photography. Since this is a very naive question it might be best if people were to reply to me directly (corbit@essex.stfx.ca) rather than to the list. Thank you very much, John John D. Corbit, Ph.D. Biology Dept. St.F.X.U. Antigonish, N.S. Canada B2G 2M5 Internet: corbit@essex.stfx.ca Voice: 902-867-2233 Fax: 902-867-5153 From wesfreid@physique.ens.fr Tue Jun 8 15:59:29 1993 Received: from nef.ens.fr by nx1.soils.umn.edu (5.65c) id AA20867; Tue, 8 Jun 1993 09:44:48 -0500 Return-Path: Received: from dmi.ens.fr by nef.ens.fr (5.65c8/ULM-1.0) Received: from peterpan.ens.fr by dmi.ens.fr (5.65c8/ULM-1.0) Received: from merlin.ens.fr by peterpan.ens.fr (4.1/88/01/19 3.0) id AA16506; Tue, 8 Jun 93 13:59:29 +0200 Date: Tue, 8 Jun 93 13:59:29 +0200 From: wesfreid@physique.ens.fr (WESFREID Eduardo) Message-Id: <9306081159.AA16506@peterpan.ens.fr> To: nih-image@soils.umn.edu Subject: problems with VCR "We purchased a Scion LG-3 frame grabber (5/93) which imports images nicely From a SIT camera but sporadically fails to import images from VCR." Received: from agcs.psu.edu (agcs.cas.psu.edu) by nx1.soils.umn.edu (5.65c) id AA20932; Tue, 8 Jun 1993 09:49:29 -0500 Return-Path: Message-Id: <199306081449.AA20932@nx1.soils.umn.edu> Date: 8 Jun 1993 09:04:31 U From: "Sylwester Chyb" Subject: color capturing with Scion LG-3 Return-Receipt-To: "Sylwester Chyb" To: nih-image@soils.umn.edu I would like to hear from those of you who have been using Scion LG-3 frame grabber for capturing color. We have just purchased Scion LG-3 frame grabber. So far everything we tested works fine but we were unable to capture color. Within weeks we expect to purchase Sony color video camera and high resolution color monitor which has 4 BNC outputs. However right now our video source has only one BNC video out. Is there a way to capture color using this output ? Sylwester Chyb Penn State Univ. From Sylwester_Chyb@agcs.psu.edu Tue Jun 8 09:50:04 1993 Received: from agcs.psu.edu (agcs.cas.psu.edu) by nx1.soils.umn.edu (5.65c) id AA20981; Tue, 8 Jun 1993 09:50:04 -0500 Return-Path: Message-Id: <199306081450.AA20981@nx1.soils.umn.edu> Date: 8 Jun 1993 09:07:30 U From: "Sylwester Chyb" Subject: multi-system VCRs Return-Receipt-To: "Sylwester Chyb" To: nih-image@soils.umn.edu We are looking for a VCR capable of handling NTSC, PAL and SECAM color systems. I would appreciate your advice on that subject (manufacturers, models, etc.) Thanks, Sylwester Chyb Penn State Univ schyb@psupen.psu.edu From mvivino@helix.nih.gov Tue Jun 8 04:32:42 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA21011; Tue, 8 Jun 1993 09:50:35 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA22515; Tue, 8 Jun 93 08:32:42 -0400 Date: Tue, 8 Jun 93 08:32:42 -0400 Message-Id: <9306081232.AA22515@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: Trouble grabbing from VCR with Scion This f >failure seems tape or recording dependent. Some recordings on Sony T-120 >ES tape import some do not. We have attempted importing from 3 different >Sony VCRs SLV400,SLV757UC,SLV595HF with similar result. Is this an intrinsic >problem to the Scion LG-3 due to its lack of time based correction or is >there a straightforward solution? The problem is not the scion board, the problem is that vcr's are not made for what you want to do. Even the very best vcr's do not compare to the output of a video camera. Generally because you watch continuosly displayed video from a vcr, the monitor and your eyes are able to compensate for the poor sync and output from these devices to some extent. However individual frame capture into a frame grabber is another whole matter. You could spend big dollars and get a vcr with tbc built in, or you can buy an external tbc. The external tbc will run the analog back to digital and then back out to analog, but properly synced. Because of all the conversions and the general low quality of vcr's anyway, this isn't the best in the world but might be all you can do. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From ruzin@nature.berkeley.edu Tue Jun 8 01:05:37 1993 Received: from nettlerash.Berkeley.EDU by nx1.soils.umn.edu (5.65c) id AA21133; Tue, 8 Jun 1993 10:01:35 -0500 Return-Path: Received: from nature.Berkeley.EDU by nettlerash.berkeley.edu (5.67.1/1.34.6) id AA12831; Tue, 8 Jun 93 08:02:52 -0700 Received: from [128.32.128.176] (kos5mac21.Berkeley.EDU) by nature.berkeley.edu.cnr-net (4.1/SMI-4.1) id AA28687; Tue, 8 Jun 93 08:05:38 PDT Date: Tue, 8 Jun 93 08:05:37 PDT Message-Id: <9306081505.AA28687@nature.berkeley.edu.cnr-net> To: From: ruzin@nature.berkeley.edu (Steven Ruzin) Subject: Re: problems with VCR >"We purchased a Scion LG-3 frame grabber (5/93) which imports images nicely >from a SIT camera but sporadically fails to import images from VCR." >We have the same problems with a Data Traslation card! > I also have the same problem. If I try to grab frames from still VTR images the synch is hopelessly lost but if I grab a frame "on the fly" then I can usually get a decent capture. I've experienced this with both the DT2255 and the LG-3. Unfortunately, my Time-lapse VTR does not have a video synch out so there's (probably) no hope of getting this right. Steve... Steven Ruzin NSF Center Dept Plant Biology Univ California Berkeley From wayne@zippy.nimh.nih.gov Tue Jun 8 11:34:31 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA21801; Tue, 8 Jun 1993 11:33:37 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA03193; Tue, 8 Jun 93 12:46:50 -0400 Message-Id: <9306081646.AA03193@zippy.nimh.nih.gov> Date: Tue, 8 Jun 1993 11:34:31 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Image can now open and edit text files I have uploaded to zippy.nimh.nih.gov a new Image beta(1.50b63) that can now open and edit text files up to 32K in size. The following command know how to deal with text: New, Open, Close, Save, Save As, Cut, Copy, Paste, Clear, Font, Size, Load Macros(loads the macros contained in the current text window). --wayne From kyzy@rice.edu Tue Jun 8 13:32:06 1993 Received: from moe.rice.edu by nx1.soils.umn.edu (5.65c) id AA23522; Tue, 8 Jun 1993 18:29:19 -0500 Return-Path: Received: from [128.42.105.7] (erinna.rice.edu) by moe.rice.edu (AA16971); Tue, 8 Jun 93 18:32:07 CDT Date: Tue, 8 Jun 93 18:32:06 CDT Message-Id: <9306082332.AA16971@moe.rice.edu> To: From: kyzy@rice.edu Subject: Re: Trouble grabbing from VCR with Scion >We purchased a Scion LG-3 frame grabber (5/93) which imports images nicely >from a SIT camera but sporadically fails to import images from VCR. This f >failure seems tape or recording dependent. Some recordings on Sony T-120 >ES tape import some do not. We have attempted importing from 3 different >Sony VCRs SLV400,SLV757UC,SLV595HF with similar result. Is this an >intrinsic problem to the Scion LG-3 due to its lack of time based >correction or is there a straightforward solution? >=============== The only straightforward solution is to use a Time Base Corrector unit (TBC) between the VCR and the frame grabber. All the problems you describe are due to the poor sync signals of the composite video output from VCRs (especially time-lapse VCRs). The TBC strips the sync, performs an analog-digital-analog conversion of the video signal and puts the correct sync back in it. Ideally, one should have a sync generator (another video gadget) driving the camera, the TBC and the frame grabber, but this is not really necessary. External TBC's have come down in price (about $ 2,000 for the last I bought) or you can buy VCR's with built in TBC. If you do not want to buy a TBC, try (as a last resort) running the frame grabber with external sync (I do not know if the Scion has this option). Then take the output from the VCR, put it into a VDA (video distribution amplifier) and connect two outputs from the VDA to the video input and the sync input of the board. This approach works with one of our frame grabbers we have installed in a MicroVAX (our pet dinosaur). It is definitely not kosher, however, and there is no guarantee it will work. But if you have a VDA around ... ------------------------------------ Prof. Kyriacos Zygourakis Dept. of Chemical Engineering Rice University Houston, Texas 77251-1892 Phone: (713) 527-8750 Ext. 3509 FAX: (713) 285-5478 ------------------------------------ From chien@jeeves.UCSD.EDU Tue Jun 8 04:26:20 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA24989; Tue, 8 Jun 1993 22:22:21 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA12318 sendmail 5.67/UCSD-2.2-sun via SMTP Tue, 8 Jun 93 11:28:13 -0700 Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA23043 to nih-image@soils.umn.edu; Tue, 8 Jun 93 11:26:20 PDT Date: Tue, 8 Jun 93 11:26:20 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306081826.AA23043@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: Re: Trouble grabbing from VCR with Scion > "We purchased a Scion LG-3 frame grabber (5/93) which imports images nicely > from a SIT camera but sporadically fails to import images from VCR." > We have the same problems with a Data Traslation card! > > J.E.Wesfreid > The problem is not the scion board, the problem is that vcr's are not made > for what you want to do. Even the very best vcr's do not compare to the > output of a video camera. > > Mark Vivino I should have mentioned that Eric also has a Data Translation board, which works much more reliably than the Scion. (He's trying to set up a second rig, which is why he bought the Scion.) The Scion LG-3 brochure says "[This board] is designed for use with high-quality CCD imaging equipment, and as such incorporates no time base correction," and I thought that this might account for the difference between the boards. Does the DT board have better time base or synching hardware? Chi-Bin Chien From corbit@esseX.stfx.ca Wed Jun 9 08:27:16 1993 Received: from esseX.StFX.CA by nx1.soils.umn.edu (5.65c) id AA26616; Wed, 9 Jun 1993 08:27:16 -0500 Return-Path: Received: by esseX.stfx.ca (MX V2.3) id 14000; Wed, 09 Jun 1993 10:29:26 AST Date: Wed, 09 Jun 1993 10:29:26 AST From: John Corbit To: nih-image@soils.umn.edu Cc: garbary@esseX.stfx.ca, parker@esseX.stfx.ca, fnkwp@acad3.alaska.edu, pdbourke@ccu1.auckland.ac.nz, A_lock@bionet.bio.dfo.ca Message-Id: <0096DC39.6AB08BC0.14000@esseX.stfx.ca> Subject: thank you! MX%"nih-image@soils.umn.edu" Thanks to all who responded to my question yesterday about what I termed "halftoning of the screen image" captured via the composite signal from a JVC color camera sent to a Scion LG-3 board in a Quadra 800 and then processed via the LG-3 plug-in by either NIH Image 1.50b52 or PhotoShop 2.5. The origin of my problem is in the camera. An especially clear statement of the situation is given by Mike Calford in the message attached below. Thanks again. Another triumph of telecommunication. A problem solved within 24 hours by worldwide sharing and collaboration. I am most grateful. John --------------------------------------------------------------------------- John D. Corbit, Ph.D. Internet: corbit@essex.stfx.ca Biology Dept. St.F.X.U. Voice: 902-867-2233 Antigonish, N.S. Fax: 902-867-5153 Canada B2G 1C0 --------------------------------------------------------------------------- --------------------------------------------------------------------------- To: corbit@essex.stfx.ca From: Mike Calford Subject: re camera Date: Wed, 9 Jun 1993 03:57:29 +0000 you are probably not getting halftones but a herring-bone type pattern over the top of your image. This is due to your use of a colour camera and the failure of the camera/board system due to the luminance signal getting through. You need to get a good monochrome CCD camera - try one of the new Panasonic models. Also don't expect a laserprinter to reproduce 256 greys. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ m.calford@mailbox.uq.oz.au Dr. M.B. Calford Vision, Touch and Hearing Research Centre The University of Queensland Brisbane, Queensland 4072 Australia From frodes@ibg.uit.no Thu Jun 10 03:47:29 1993 Received: from benoni.Uit.No by nx1.soils.umn.edu (5.65c) id AA00506; Thu, 10 Jun 1993 01:47:15 -0500 Return-Path: Received: from benoni by ppenoni.uit.no with SMTP (PP) id <06701-0@ppenoni.uit.no>; Thu, 10 Jun 1993 08:49:56 +0000 Received: from darwin.Ibg.Uit.No by benoni.uit.no (5.65+IDA/Babel-1.15/ABaa-1.2/Ultrix) id AAbenoni06697; Thu, 10 Jun 1993 08:49:54 +0200 Received: from [129.242.200.137] (mac-if.Ibg.Uit.No) by darwin.ibg.uit.no (4.1/ABaa-2.0mini) id AA04881; Thu, 10 Jun 93 08:44:40 +0200 Message-Id: <9306100644.AA04881@darwin.ibg.uit.no> Date: Thu, 10 Jun 1993 08:47:29 -0500 To: NIH-IMAGE@soils.umn.edu From: frodes@ibg.uit.no (Frode Skarstein) X-Sender: frodes@darwin.ibg.uit.no REWIEW Torfinn """"""" From frodes@ibg.uit.no Thu Jun 10 09:49:09 1993 Received: from benoni.Uit.No by nx1.soils.umn.edu (5.65c) id AA00909; Thu, 10 Jun 1993 03:41:04 -0500 Return-Path: Received: from benoni by ppenoni.uit.no with SMTP (PP) id <07468-0@ppenoni.uit.no>; Thu, 10 Jun 1993 10:43:50 +0000 Received: from darwin.Ibg.Uit.No by benoni.uit.no (5.65+IDA/Babel-1.15/ABaa-1.2/Ultrix) id AAbenoni07464; Thu, 10 Jun 1993 10:43:48 +0200 Received: from [129.242.200.140] by darwin.ibg.uit.no (4.1/ABaa-2.0mini) id AA05095; Thu, 10 Jun 93 10:38:34 +0200 Message-Id: <9306100838.AA05095@darwin.ibg.uit.no> Date: Thu, 10 Jun 1993 09:49:09 +0000 To: nih-image@soils.umn.edu From: frodes@ibg.uit.no (Frode Skarstein) X-Sender: frodes@darwin.ibg.uit.no REWIEW From mvivino@helix.nih.gov Thu Jun 10 04:51:26 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA01633; Thu, 10 Jun 1993 07:48:38 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA13037; Thu, 10 Jun 93 08:51:26 -0400 Date: Thu, 10 Jun 93 08:51:26 -0400 Message-Id: <9306101251.AA13037@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Unique frame grabber on the market Found out about this yesterday, so I thought others might be interested. I had no previous knowledge of them. Wayne, you might want to include this one in your frame grabber co list. A company called Precision Digital Images Co called me with some technical questions about Image. They are makers of a unique frame grabbing device. It can sample normal video, but can also sample analog data at a rate up to 115 MHz and place the result into an image. Such things are used in nuclear med, MRI or any other nonstandard image acqusitions. It can also acquire up to 2K by 2K images (8 bit), or of course small images (also 8 bit). The board is called Immaxx. They are in the process of developing a photoshop plug-in and want it to work with Image since they have had requests for that. I have no connection with the company and haven't seen their product but if anyone is interested: Precision Digital Images Co 8711 148th Ave, NE Redmond, WA 98052 206-867-9177 Mark Vivino NIH/DCRT mvivino@helix.nih.gov From kartenh@Sdsc.Edu Thu Jun 10 17:22:42 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA03232; Thu, 10 Jun 1993 12:37:39 -0500 Return-Path: Date: Thu, 10 Jun 93 17:22:42 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930610172242.26246205@sds.sdsc.edu> Subject: Re: problem with grabbing To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH The QckCapInit 01.01 is not needed to run Image. Remove it!. It is probably the cource of your problem. Harvey Karten From kartenh@Sdsc.Edu Thu Jun 10 17:46:34 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA03400; Thu, 10 Jun 1993 13:00:21 -0500 Return-Path: Date: Thu, 10 Jun 93 17:46:34 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930610174634.26246205@sds.sdsc.edu> Subject: Re: light integration with CCD camera To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH The Dage DSP 200 allows image integration with any CCD camera. We use it with a Dage 72/SONY XC77 (same camera, but the Dage unit allow you to externally switch off and on the AGC. ) We find that it allows us to grab images of relatively low light levels that otherwise could not be captured with an ordinary CCD. But I don't know the absolute increase in efficiency. The DSP 200 allows either automatic integration, or frame by frame integration under manual control. It then saves it in a buffer and then can be digitized by the computer. The newer verrsion of the DAGE DSP reportedly has better capability to allow the computer to control the whole process. Harvey Karten From kartenh@Sdsc.Edu Thu Jun 10 17:57:00 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA03729; Thu, 10 Jun 1993 13:23:55 -0500 Return-Path: Date: Thu, 10 Jun 93 17:57:00 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930610175700.26246205@sds.sdsc.edu> Subject: Re: Trouble grabbing from VCR with Scion To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH The solution to the problem of TBC from a VCR to a frame grabber may be soluble using the SONY Hi-8 Digital VCR. It costs about $1,200, and I believe it has a built in TBC. Joe Ayers showed it to me in operation recently and the results were most impressive. I believe it is a model 3000. Write to Joe Ayers for more info. From John_Mansfield@mse.engin.umich.edu Thu Jun 10 13:26:59 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA03797; Thu, 10 Jun 1993 13:26:59 -0500 Return-Path: Message-Id: <199306101826.AA03797@nx1.soils.umn.edu> Date: 10 Jun 1993 14:30:43 U From: "John Mansfield" Subject: Re: light integration with To: nih-image@soils.umn.edu Reply_ RE>>light integration with C Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu >The Dage DSP 200 allows image integration with any CCD camera. We >use it with a Dage 72/SONY XC77 (same camera, but the Dage unit allow you >to The trouble with using the DSP box to average the images is that you now have the chain: analog -> digital -> analog -> digital camera->dsp->monitor->digitizer. Not the most efficient way og doing things. Just my 10cents worth! Jfm From kartenh@Sdsc.Edu Thu Jun 10 20:54:21 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA04721; Thu, 10 Jun 1993 15:51:54 -0500 Return-Path: Date: Thu, 10 Jun 93 20:54:21 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930610205421.26246205@sds.sdsc.edu> Subject: Re: light integration with To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH " jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu >The Dage DSP 200 allows image integration with any CCD camera. We >use it with a Dage 72/SONY XC77 (same camera, but the Dage unit allow you >to The trouble with using the DSP box to average the images is that you now have the chain: analog -> digital -> analog -> digital camera->dsp->monitor->digitizer. Not the most efficient way og doing things. Just my 10cents worth! Jfm" You are correct, and Dage has repeatedly reneged on the promise to provide a direct digital output from the DSP 200. But It does provide a solution. The thing that also stands out is the quality of the digitized image coming from the DSP is fairly crisp. harvey karten From bsimon@pennsy.med.jhu.edu Thu Jun 10 13:09:48 1993 Received: from pennsy.med.jhu.edu by nx1.soils.umn.edu (5.65c) id AA04765; Thu, 10 Jun 1993 15:55:03 -0500 Return-Path: Received: from [128.220.90.219] by pennsy.med.jhu.edu (5.65/1.34) id AA21778; Thu, 10 Jun 93 17:09:48 -0400 Date: Thu, 10 Jun 93 17:09:48 -0400 Message-Id: <9306102109.AA21778@pennsy.med.jhu.edu> To: nih-image@soils.umn.edu From: bsimon@pennsy.med.jhu.edu (Brett A. Simon) Subject: Header offset GE9800 CT Does anyone know the offset for images from the GE 9800 CT scanner? -Thanks- please reply directly to bsimon@pennsy.med.jhu.edu _____ Brett Simon (bsimon@pennsy.med.jhu.edu) Dept. of Anesthesia and Critical Care Medicine Johns Hopkins Hospital (410) 614-1515 From wching@alice.music.yale.edu Thu Jun 10 15:07:28 1993 Received: from ALICE.MUSIC.YALE.EDU by nx1.soils.umn.edu (5.65c) id AA05605; Thu, 10 Jun 1993 18:04:47 -0500 Return-Path: Received: by alice.music.yale.edu (NX5.67c/NX3.0M) id AA06145; Thu, 10 Jun 93 19:07:28 -0400 Date: Thu, 10 Jun 93 19:07:28 -0400 From: William Ching Message-Id: <9306102307.AA06145@alice.music.yale.edu> To: nih-image@soils.umn.edu Subject: Is there a macro interface to the Surface Plot fn (Analyze menu)? Hi fellow Image users! I have a question regarding the macro language and the Surface Plot function in Image 1.50b63. I was wondering if there was a macro hook for the Surface Plot function (I'm trying to automate the creation of a stack of surface plots). If anyone has any ideas or suggestions, I'd be really appreciative for any help. Thanks! ------------------------------------------------------------------------------ William Ching | Yale University School of Medicine ching-william@yale.edu | Neuroscience and Regeneration Center "I didn't inhale..." - Billster '93 | VA Medical Center, West Haven, CT 06516 From scudder@cpac.washington.edu Thu Jun 10 11:25:06 1993 Received: from bailey.cpac.washington.edu by nx1.soils.umn.edu (5.65c) id AA06338; Thu, 10 Jun 1993 20:22:20 -0500 Return-Path: Received: by bailey.cpac.washington.edu (5.65/UW-NDC Revision: 2.4 ) id AA20531; Thu, 10 Jun 93 18:25:15 -0700 Date: Thu, 10 Jun 1993 18:25:06 -0700 (PDT) From: Kurt Scudder Sender: Kurt Scudder Reply-To: Kurt Scudder Subject: NTSC video from the Mac? To: image-mail Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII I've been trying to get up to speed on what people have done with respect to getting video output from the Mac and from NIH Image in particular. I've read the quadra and centris video text files on zippy, plus the discussion in the NEC PC VCR macro in the /macros directory on zippy. Evidently there are ways to get monochrome video out, but not color. My interest is not just in sending processed images out of NIH Image, but also whether this could be used as a cheap way to do video titling for archival or demo tapes. Mac Warehouse sells an item called a "TV Encoder" for US$299 which supposedly will provide NTSC video with the same color depth as the Mac video card which drives it (i.e., 256-color or 24-bit-color, but it will not work with the built-in video on the IIci, IIsi, or LC). Is this a possible solution? If not, why not? I understand that one must use a separate monitor for the image windows so as not to get the title bar on the videotape as well. Another possible solution: There was an article in Radio-Electronics back in 1991 on the construction of a VGA-to-NTSC converter ("PC to TV Converter",Radio-Electronics vol. 62, no. 10, Oct 1991, pp 33-8). This device actually converted the analog RGB coming out of the VGA connector to NTSC through some special video-processing chips. The kit price was about US$160, but I think you could also buy a completed version. It went on a card in the PC, but this was only to get DC power and to generate an interrupt for some reason which I can't recall. Kind of funky: the VGA signal comes out of the video card and goes right back into another card slot; another cable connects the card to the VGA monitor, and a third cable goes to the TV or VCR..The VGA and the Mac video (at least the Mac II/IIx/IIci) aren't all that different, are they? Could such a circuit be modified to work with the Mac video and keep the color info? Provided this would work, It seems relatively straightforward to put Mac video on tape. Now let's suppose I want to take some existing videotape and copy it to another machine, dubbing in my titles at the appropriate spot. The titles are just still text frames. What additional equipment do I need to do this? Can I just use a video switch, or do I have to get the two sources to have a common sync? How is this done commercially? What about overlaying two video signals? If anyone can help or has ideas, I'd like to hear them. Kurt Scudder Dept. of Chemistry University of Washington Seattle, WA 98195 scudder@u.washington.edu From pdevries@macc.wisc.edu Fri Jun 11 09:31:13 1993 Received: from vms2.macc.wisc.edu by nx1.soils.umn.edu (5.65c) id AA09990; Fri, 11 Jun 1993 09:31:13 -0500 Return-Path: Date: Fri, 11 Jun 1993 09:31:13 -0500 Received: from rubble.bocklabs.wisc.edu by vms2.macc.wisc.edu; Fri, 11 Jun 93 09:32 CDT Message-Id: <23061109324145@vms2.macc.wisc.edu> From: (Pete DeVries) Subject: Re: NTSC video from the Mac? To: nih-image@soils.umn.edu X-Sender: pdevries@vms2.macc.wisc.edu > > I've been trying to get up to speed on what people have done with >respect to getting video output from the Mac and from NIH Image in >particular. We have been using a Radius Videovision board to output the Mac video to video tape. It works very well. One particular advantage is the flicker removing convolution function built into the board. The Mac video is non-interlaced, while NTSC is interlaced. The result is that certain areas of the screen will flicker when put to tape. The Videovision's convolution function will eliminate the flicker. You can turn the convolution filter on or off from the Monitors control panel. The board supports 13" monitors at 24-bit. To change from the Mac screen to the TV screen you simply click a button in the Monitors control panel. You don't need to change cables each time - it is done in software. The board will also capture stills, Quicktime movies, and audio. It costs approximately $1600. There is a daughter board available that will allow you to capture 640x480 Quicktime movies at 30fps. If you are going to be doing a lot of recording of the Mac screen, and don't want to have to deal with the annoying flicker, this is the way to go. There is nothing like it in its price range. -Pete ------------------------------------------------ Pete DeVries Information Processing Consultant University of Wisconsin - Madison Laboratory of Molecular Biology (608) 262-3403 Integrated Microscopy Resource (608) 263-6288 Email: pdevries@macc.wisc.edu FAX (608) 262-4570 ------------------------------------------------- From wayne@zippy.nimh.nih.gov Fri Jun 11 10:14:53 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA10346; Fri, 11 Jun 1993 10:14:05 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA07840; Fri, 11 Jun 93 11:27:17 -0400 Message-Id: <9306111527.AA07840@zippy.nimh.nih.gov> Date: Fri, 11 Jun 1993 10:14:53 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Is there a macro interface to the Surface Plot fn (Analyze menu)? >Hi fellow Image users! > I have a question regarding the macro language and the Surface Plot function >in Image 1.50b63. I was wondering if there was a macro hook for the Surface >Plot function (I'm trying to automate the creation of a stack of surface >plots). If anyone has any ideas or suggestions, I'd be really appreciative >for any help. Thanks! Here is a macro that creates a surface plot movie from a stack. It requires V1.50b70, which is available by ftp from zippy.nimh.nih.gov. --wayne macro 'Make Surface Plot Movie '; var i,OldStack,NewStack:integer; N,PlotWidth,PlotHeight:integer; ScaleFactor:real; OneToOne:boolean; begin RequiresVersion(1.50); CheckForStack; SaveState; OldStack:=PicNumber; N:=nSlices; PlotWidth:=GetNumber('Surface Plot Width:',300); PlotHeight:=GetNumber('Surface Plot Width:',300); SetNewSize(PlotWidth,PlotHeight); MakeNewStack('Stack'); NewStack:=PicNumber; SelectPic(OldStack); for i:= 1 to N do begin SelectSlice(1); SurfacePlot; SelectAll; Copy; Dispose; SelectPic(NewStack); if i<>1 then AddSlice; Paste; SelectPic(OldStack); DeleteSlice; end; Dispose; {OldStack} RestoreState; end; From cammer@alsys1.aecom.yu.edu Fri Jun 11 07:22:33 1993 Received: from alsys1.aecom.yu.edu by nx1.soils.umn.edu (5.65c) id AA10480; Fri, 11 Jun 1993 10:22:00 -0500 Return-Path: Received: by alsys1.aecom.yu.edu id AA14222 (5.65c/IDA-1.4.4/AECOM-RIT for nih-image@soils.umn.edu); Fri, 11 Jun 1993 11:24:47 -0400 Date: Fri, 11 Jun 1993 11:22:33 -0400 (EDT) From: Michael Cammer Subject: Ektron-1400 plug in for Adobe Photoshop To: nih-image@soils.umn.edu Cc: Michael Cammer In-Reply-To: <9306111527.AA07840@zippy.nimh.nih.gov> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII We just uploaded the most recent version of Image (1.50b...) and find that the Adobe Photoshop plug in module for scanning from an Ektron 1400 series camera works fine. From Sylwester_Chyb@agcs.psu.edu Fri Jun 11 10:29:18 1993 Received: from agcs.psu.edu (agcs.cas.psu.edu) by nx1.soils.umn.edu (5.65c) id AA10599; Fri, 11 Jun 1993 10:29:18 -0500 Return-Path: Message-Id: <199306111529.AA10599@nx1.soils.umn.edu> Date: 11 Jun 1993 11:33:57 U From: "Sylwester Chyb" Subject: loading macros at Image startup Return-Receipt-To: "Sylwester Chyb" To: nih-image@soils.umn.edu Is there a way to load a macro file at Image startup ? Thanks for help, Sylwester Chyb Dept. Entomology, Penn State University, University Park, PA 16802 From wayne@zippy.nimh.nih.gov Fri Jun 11 10:50:10 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA10832; Fri, 11 Jun 1993 10:49:22 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA07918; Fri, 11 Jun 93 12:02:34 -0400 Message-Id: <9306111602.AA07918@zippy.nimh.nih.gov> Date: Fri, 11 Jun 1993 10:50:10 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: loading macros at Image startup >Is there a way to load a macro file at Image startup ? >Thanks for help, >Sylwester Chyb >Dept. Entomology, >Penn State University, >University Park, PA 16802 When launched, Image automatically loads the macros contained in the file "Image Macros", as long as this file is in the same folder as Image, or in the System Folder. The current version of NIH Image(V1.49), including documentation, example images and complete Think Pascal source code, is available by anonymous ftp From zippy.nimh.nih.gov[128.231.98.32], in the directory /pub/image. Received: from bham.ac.uk by nx1.soils.umn.edu (5.65c) id AA22176; Sun, 13 Jun 1993 08:11:11 -0500 Return-Path: Via: uk.ac.birmingham.computer-centre.ibm3090; Sun, 13 Jun 1993 14:02:55 +0100 Date: Sun, 13 Jun 93 14:12:37 BST From: DAVIESDA@ibm3090.computer-centre.birmingham.ac.uk Subject: Image and Radius VideoVision To: nih-image@SOILS.UMN.EDU Message-Id: <"13_Jun_93_14:12:37_BST_#7747"@UK.AC.BHAM.IBM3090> Does anyone know how I can get Image to grab video directly from my VideoVision board rather than first having to grab something using the supplied Radius software then importing it into Image? Thanks, David Davies. Physiology Department, Birmingham University, UK. d.a.davies@uk.ac.bham From contper1@NKI.NL Fri Jun 14 12:06:00 1993 Received: from vx.cis.umn.edu by nx1.soils.umn.edu (5.65c) id AA03921; Mon, 14 Jun 1993 04:03:52 -0500 Return-Path: Received: from HASARA5.BITNET (MAILGATE@HASARA5) by vx.cis.umn.edu (PMDF #2574 ) id <01GZCSH74JS09QUSG1@vx.cis.umn.edu>; Mon, 14 Jun 1993 04:07:51 CDT Received: from SARA.NL by SARA.NL for nih-image@nx1.soils.umn.edu; 14 Jun 93 11:06 MET Received: from NKI.NL by SARA.NL with PMDF#10201; Mon, 14 Jun 1993 11:06 MET Received: from [192.42.114.110] by NKI.NL; Mon, 14 Jun 93 11:05 GMT Date: 14 Jun 1993 11:06 +0100 (MET) From: contper1@NKI.NL Subject: RE:RE: problem with grabbing To: nih-image@soils.umn.edu Message-Id: <1FD3B670D79F0027ED@NKI.NL> X-Envelope-To: nih-image@nx1.soils.umn.edu Content-Transfer-Encoding: 7BIT Date-Note: Date: header was inserted by SARA.NL X-Sender: contper1@nki.nl (Unverified) Thanks to all who replied about the problem of grabbing images with the Data Translation card. None of your suggestions had any effect. >From the representative in The Netherlands (Rood, Rijswijk) I got a new board and everything was o.k.! The one I used was an old one (about six years old). The QuickCapInit I need for the image processing program TCL-Image, developed by the TNO Institute of Applied Phisics and Multihouse-TSI. Ilja. ------------------------------------------------------------------------------ ilja van de pavert antoni van leeuwenhoekhuis/the netherlands cancer institute dept. of biophysics plesmanlaan 121 phone: +31 20 5121894 1066 cx amsterdam fax: +31 20 5121944 the netherlands email: contper1@nki.nl ---------------------------------------------------------------------------- -- From burk@aecom.yu.edu Mon Jun 14 11:39:14 1993 Received: from alsys1.aecom.yu.edu by nx1.soils.umn.edu (5.65c) id AA00584; Mon, 14 Jun 1993 09:31:47 -0500 Return-Path: Received: from [129.98.60.103] (hachma.aecom.yu.edu) by alsys1.aecom.yu.edu with SMTP id AA29331 (5.65c/IDA-1.4.4/AECOM-RIT for ); Mon, 14 Jun 1993 10:35:14 -0400 Message-Id: <199306141435.AA29331@alsys1.aecom.yu.edu> Date: Mon, 14 Jun 1993 10:39:14 +0100 To: nih-image@soils.umn.edu From: burk@aecom.yu.edu Subject: Radius Rocket 33 compatibility with image Before I upgrade my MacII with the Rocket33 Accelerator for use with image analysis I want to determine whether anyone has experience with this hardware and its compatibility. Robbie Burk Robert D. Burk, M.D. burk@aecom.yu.edu From glenmac@u.washington.edu Mon Jun 14 06:51:02 1993 Received: from carson.u.washington.edu by nx1.soils.umn.edu (5.65c) id AA03159; Mon, 14 Jun 1993 15:59:22 -0500 Return-Path: Received: by carson.u.washington.edu (5.65/UW-NDC Revision: 2.22 ) id AA23155; Mon, 14 Jun 93 14:02:45 -0700 Sender: glenmac@carson.u.washington.edu Date: Mon, 14 Jun 1993 13:51:02 -0700 (PDT) From: Glen Macdonald Subject: Cavalieri macro To: nih-image@soils.umn.edu Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=us-ascii In response to email inquiries regarding quantitative morphometry I posted 'Cavalieri_Macro' and 'Cavalieri_Doc' to Zippy in the Image/Contrib folder. Both are Word text files. The macro is a general purpose tool (we have many variants for specific projects) that allows point counting estimates of area and volume. It also contains a reference frame for object (usually cell) counts using optical or physical disector methods. Eventually, I will hookup a serial port routine to control a Heidenhain VRZ 405 length guage for auto-recording depth of focus and providing an audible cue when reaching a pre-set focus depth for optical disector counting. There are references given in the accompanying doc file. Any bugs, questions or suggestions for improvements greatly appreciated. Glen MacDonald glenmac@u.washington.edu Hearing Development Laboratories, RL-30 University of Washington Seattle, WA 98195 From @cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be Tue Jun 15 10:24:34 1993 Received: from cc1.kuleuven.ac.be by nx1.soils.umn.edu (5.65c) id AA08847; Tue, 15 Jun 1993 10:24:34 -0500 Return-Path: <@cc1.kuleuven.ac.be:jvanheld@dbm.ulb.ac.be> Received: from rc1.vub.ac.be by cc1.kuleuven.ac.be (IBM VM SMTP V2R2) with TCP; Tue, 15 Jun 93 17:28:14 +0200 Received: from dec5.ulb.ac.be (dbmdec5) by rc1.vub.ac.be (4.1/RC1-930609) id AA16537; Tue, 15 Jun 93 17:28:32 +0200 Received: by dec5.ulb.ac.be (5.65/ULB.920908) id AA20826; Tue, 15 Jun 1993 17:24:45 +0100 From: jvanheld@dbm.ulb.ac.be (Jacques VAN HELDEN) Message-Id: <9306151624.AA20826@dec5.ulb.ac.be> Subject: automatic cell edge finding To: NIH-IMAGE@soils.umn.edu Date: Tue, 15 Jun 93 17:24:44 WET DST Cc: jvanheld@dbm.ulb.ac.be (Jacques VAN HELDEN) X-Mailer: ELM [version 2.3 PL11] Has anyone experience with automatic contourning of cells on microscope images captured in differential interference contrast (DIC, or Nomarski optics) ? Thanks, Jacques van Helden jvanheld@dbm.ulb.ac.be From burk@aecom.yu.edu Tue Jun 15 12:47:06 1993 Received: from alsys1.aecom.yu.edu by nx1.soils.umn.edu (5.65c) id AA09448; Tue, 15 Jun 1993 10:39:39 -0500 Return-Path: Received: from [129.98.60.103] (hachma.aecom.yu.edu) by alsys1.aecom.yu.edu with SMTP id AA01666 (5.65c/IDA-1.4.4/AECOM-RIT for ); Tue, 15 Jun 1993 11:43:02 -0400 Message-Id: <199306151543.AA01666@alsys1.aecom.yu.edu> Date: Tue, 15 Jun 1993 11:47:06 +0100 To: nih-image@soils.umn.edu From: burk@aecom.yu.edu Subject: radius 33 accelerator and image. I would like to up-grade my MacII and I'm considering the radium rocket 33 accelerator. Does any one the compatibility of this hardware with image, or have other suggestions for up-grading. Robbie Burk Robert D. Burk, M.D. burk@aecom.yu.edu From STPREMW@production.unocal.com Tue Jun 15 02:21:00 1993 Received: from unogate.unocal.com by nx1.soils.umn.edu (5.65c) id AA09723; Tue, 15 Jun 1993 11:24:43 -0500 Return-Path: Received: from st.unocal.com (unocal-3.st.unocal.com) by unogate.unocal.com (4.1/SMI-4.1) id AA26864; Tue, 15 Jun 93 09:28:36 PDT Received: from courier by st.unocal.com (4.1/SMI-4.1) id AA25980; Tue, 15 Jun 93 09:28:28 PDT Received: by courier with Microsoft Mail id <2C1DF793@courier>; Tue, 15 Jun 93 09:23:47 PDT From: "Withjack, Eric" To: nih-image@soils.umn.edu Subject: Header offset GE9800 CT Date: Tue, 15 Jun 93 09:21:00 PDT Message-Id: <2C1DF793@courier> Encoding: 31 TEXT X-Mailer: Microsoft Mail V3.0 I suggest that you contact GE for this info since they consider it proprietary. Years ago, I obtained this info from GE medical systems in Milwaukee but signed a non-disclosure agreement. I've lost contact with most of these folks, but you may try getting in contact with Dr. Mike Dennis who is now in Cincinnati (GE NDE Systems) at 513 551-1590...this is a 4yr old number??? Good luck. ------------------------------------------------------------------------------ REPLY FROM: Withjack, Eric Return-Path: Errors-To: owner-nih-image@soils.umn.edu Date: Thu, 10 Jun 1993 16:01:41 -0500 Message-Id: <9306102109.AA21778@pennsy.med.jhu.edu> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: bsimon%pennsy.med.jhu.edu@relay.tc.umn.edu (Brett A. Simon) To: Multiple recipients of list Subject: Header offset GE9800 CT Does anyone know the offset for images from the GE 9800 CT scanner? -Thanks- please reply directly to bsimon@pennsy.med.jhu.edu _____ Brett Simon (bsimon@pennsy.med.jhu.edu) Dept. of Anesthesia and Critical Care Medicine Johns Hopkins Hospital (410) 614-1515 From gmei@hoh.mbl.edu Tue Jun 15 13:48:21 1993 Received: from AQUA.WHOI.EDU by nx1.soils.umn.edu (5.65c) id AA11235; Tue, 15 Jun 1993 16:50:28 -0500 Return-Path: Received: by aqua.whoi.edu (5.57/Ultrix3.0-C) id AA03657; Tue, 15 Jun 93 17:53:49 -0400 Received: from [128.128.172.217] (STARMAC17.MBL.EDU) by hoh.mbl.edu. (4.1/SMI-4.1) id AA03129; Tue, 15 Jun 93 17:48:24 EDT Date: Tue, 15 Jun 93 17:48:21 EDT Message-Id: <9306152148.AA03129@hoh.mbl.edu.> To: nih-image@soils.umn.edu From: gmei@hoh.mbl.edu (Guang Mei) Subject: call for help This is an inquiry and call for help: We have a Scion LG-3 frame grabber board with 16 MB of video RAM. We need to capture several (approx. 30) frames at video rate and subsequently transfer the frames to a stack (without averaging). Start and stop capturing commands should be two different macro commands. The stop command should include the transfer of all the frames in the LG-3 video RAM to a stack in the computer RAM. Also, during the video rate capturing the settings of the two Digital to Analog converters on the LG-3 (Scion[1] and Scion [2]) need to be changed independently, without affecting the video rate capture. Has anyone implemented such macro functions (or similar ones)? Who else is interested in such macro functions? Wayne, would you consider implementing these functions? ------------- Guang Mei gmei@hoh.mbl.edu Tel:(508)548-3705 ext 374 Program of Architectural Dynamics in Living Cells Marine Biological Laboratory, Woods Hole, MA 02543-1031 From huff@MCCLB0.MED.NYU.EDU Wed Jun 16 17:43:14 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA20141; Wed, 16 Jun 1993 21:39:55 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GZGO0QN8F40007SW@MCCLB0.MED.NYU.EDU>; Wed, 16 Jun 1993 22:43:16 EDT Date: Wed, 16 Jun 1993 22:43:14 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Re: NTSC video from the Mac? To: nih-image@soils.umn.edu Message-Id: <01GZGO0R03DU0007SW@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT Just going through my mail file in reverse order and came to this message. We have successfully recorded color video and color S-video using the NEC PC VCR and the Computer Video TV encoder. It's nice to know that we actually needed to buy that box, since I was getting the impression that actually all I needed was to make a cable. My video tape had Mac titlebars on the window... > I've been trying to get up to speed on what people have done with >respect to getting video output from the Mac and from NIH Image in >particular. I've read the quadra and centris video text files on zippy, >plus the discussion in the NEC PC VCR macro in the /macros directory on >zippy. Evidently there are ways to get monochrome video out, but not >color. My interest is not just in sending processed images out of NIH >Image, but also whether this could be used as a cheap way to do video >titling for archival or demo tapes. > Mac Warehouse sells an item called a "TV Encoder" for US$299 which >supposedly will provide NTSC video with the same color depth as the Mac >video card which drives it (i.e., 256-color or 24-bit-color, but it will >not work with the built-in video on the IIci, IIsi, or LC). Is this a >possible solution? If not, why not? I understand that one must use a >separate monitor for the image windows so as not to get the title bar on >the videotape as well. > Another possible solution: There was an article in >Radio-Electronics back in 1991 on the construction of a VGA-to-NTSC >converter ("PC to TV Converter",Radio-Electronics vol. 62, no. 10, Oct >1991, pp 33-8). This device actually converted the analog RGB coming out >of the VGA connector to NTSC through some special video-processing chips. >The kit price was about US$160, but I think you could also buy a completed >version. It went on a card in the PC, but this was only to get DC power >and to generate an interrupt for some reason which I can't recall. Kind of >funky: the VGA signal comes out of the video card and goes right back into >another card slot; another cable connects the card to the VGA monitor, and >a third cable goes to the TV or VCR..The VGA and the Mac video (at least >the Mac II/IIx/IIci) aren't all that different, are they? Could such a >circuit be modified to work with the Mac video and keep the color info? > Provided this would work, It seems relatively straightforward to >put Mac video on tape. Now let's suppose I want to take some existing >videotape and copy it to another machine, dubbing in my titles at the >appropriate spot. The titles are just still text frames. What additional >equipment do I need to do this? Can I just use a video switch, or do I >have to get the two sources to have a common sync? How is this done >commercially? What about overlaying two video signals? If anyone can help >or has ideas, I'd like to hear them. > >Kurt Scudder >Dept. of Chemistry >University of Washington >Seattle, WA 98195 >scudder@u.washington.edu -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From jmorrow@pirl.lpl.arizona.edu Thu Jun 17 00:55:52 1993 Received: from Arizona.edu (Osprey.Telcom.Arizona.EDU) by nx1.soils.umn.edu (5.65c) id AA22770; Thu, 17 Jun 1993 09:53:15 -0500 Return-Path: Received: from pirl.lpl.arizona.edu by Arizona.edu (PMDF V4.2-11 #2381) id <01GZH7BKIK0WA4M7XZ@Arizona.edu>; Thu, 17 Jun 1993 07:56:24 MST Received: by pirl.lpl.arizona.edu (4.1/SMI-3.2 LPL-MCN 1.25 91/08/23) id AA10846; Thu, 17 Jun 93 07:55:52 MST Date: Thu, 17 Jun 1993 07:55:52 -0700 (MST) From: jmorrow@pirl.lpl.arizona.edu (John Morrow) To: nih-image@soils.umn.edu Message-Id: <9306171455.AA10846@pirl.lpl.arizona.edu> Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT subscribe nih-image From 00A0919@MSGATE.EMIS.hac.com Mon Jun 17 09:04:09 1993 Received: from hac2arpa.hac.com by nx1.soils.umn.edu (5.65c) id AA23277; Thu, 17 Jun 1993 11:04:18 -0500 Return-Path: <00A0919@MSGATE.EMIS.hac.com> Received: from GSGMVS.EMIS.HAC.COM ([192.27.11.14]) by hac2arpa.hac.com (4.1/SMI-DDN) id AA05523; Thu, 17 Jun 93 09:07:39 PDT Received: from MSGATE.EMIS.HAC.COM by GSGMVS.EMIS.HAC.COM (Soft-Switch Central V4L380P3); 17 Jun 1993 09:04:09 GMT Message-Id: Date: 17 Jun 1993 09:04:09 GMT From: "Sumida, David S" <00A0919@MSGATE.EMIS.hac.com> Subject: RE: Radius Rocket 33 compatibility with image To: nih-image@soils.umn.edu Comment: MEMO 06.17.93 09.04 ----- ITEM 1 ----- From: 00A0919@MRAM_EDEN@EDEN Author: Sumida, David S To: NIHIMAGE@SMTPGATE@SSW@EDEN Created: // Time: :: Subject: RE: Radius Rocket 33 compatibility with image To : eden::SSW::SMTPGATE::NIHIMAGE Date: 17-JUN-1993 09:02:54.00 >Before I upgrade my MacII with the Rocket33 Accelerator for use with image >analysis I want to determine whether anyone has experience with this >hardware and its compatibility. In partial answer to Robbie Burk's question: As I mentioned earlier in another message to the listserv, I have upgraded my MacII with a Daystar Digital Universal PowerCache 030 running at 50 MHz and have found no incompatibility with software applications thusfar (i.e. Image, Word, Labview, etc). I chose the Daystar over the Rocket33 because of strong recommendations from the computer technical staff here. One of the advantages of the Daystar is that they offer upward compatibility/trade-ins with their newer Turbo 040 products as they become available for the different Mac platforms. David Sumida, PhD 00a0919@msgate.emis.hac.com From afruppe@pxdvgx.erenj.com Thu Jun 17 09:23:43 1993 Received: from erenj.com (ereapp.erenj.com) by nx1.soils.umn.edu (5.65c) id AA24299; Thu, 17 Jun 1993 13:18:36 -0500 Return-Path: Received: by erenj.com (5.65/mjr-920806); id AA07617; Thu, 17 Jun 93 14:21:54 -0400 Received: by eredns.erenj.com (5.65/bdb-mailv1.3-erenj); id AA28199; Thu, 17 Jun 93 14:21:51 -0400 Posted-Date: Thu, 17 Jun 1993 14:23:43 -0500 Message-Id: <9306171821.AA28199@eredns.erenj.com> Date: Thu, 17 Jun 1993 14:23:43 -0500 To: From: afruppe@pxdvgx.erenj.com (Albert Ruppert) Subject: Re: Radius Rocket 33 compatibility with image >Before I upgrade my MacII with the Rocket33 Accelerator for use with image >analysis I want to determine whether anyone has experience with this >hardware and its compatibility. > >Robbie Burk >Robert D. Burk, M.D. > > > > >burk@aecom.yu.edu Robbie, I have a IIx with a Radius (21") monitor. I recently installed the Rocket 33 with 20 Megs RAM. It's a like new machine. Radius includes some extensions for graphics (completely transparent to my use) that have upgraded the interface (eg. writing to the screen) to such an extent that I become impatient when I have to use the 68030 cpu for certain applications. I have used Image since I upgraded with the Rocket and I have detected no problems. I could say more but I don't want this to sound like an ad for Radius. Send me an e-mail if you want more information. =========================================================================== All thoughts expressed are mine and are not intended to reflect the opinions of my employer. (disclaimer) Al Ruppert afruppe@pxdvgx.erenj.com (908) 730-2979 From wayne@zippy.nimh.nih.gov Thu Jun 17 15:30:17 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA25143; Thu, 17 Jun 1993 15:28:54 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (16.7/zippy-1.0) id AA00622; Thu, 17 Jun 93 16:42:52 -0400 Message-Id: <9306172042.AA00622@zippy.nimh.nih.gov> Date: Thu, 17 Jun 1993 15:30:17 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Video rate capture using Scion LG-3 >We have a Scion LG-3 frame grabber board with 16 MB of video RAM. > >We need to capture several (approx. 30) frames at video rate and >subsequently transfer the frames to a stack (without averaging). Start and >stop capturing commands should be two different macro commands. The stop >command should include the transfer of all the frames in the LG-3 video RAM >to a stack in the computer RAM. I am planning to add a MakeMovie macro command that will have the ability capture at 30fps using the Scion LG-3's built-in memory. >Also, during the video rate capturing the settings of the two Digital to >Analog converters on the LG-3 (Scion[1] and Scion [2]) need to be changed >independently, without affecting the video rate capture. You should be able to set the two D/A's independently of each other by assigning values to either Scion[1] or Scion[2]. --wayne p.s. I just upload v1.40b75, which has the ability to import 16-bit TIFF files. From huff@MCCLB0.MED.NYU.EDU Thu Jun 17 13:57:53 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA25963; Thu, 17 Jun 1993 17:54:42 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-10 #2884) id <01GZHUFPJL9C000BW6@MCCLB0.MED.NYU.EDU>; Thu, 17 Jun 1993 18:57:55 EDT Date: Thu, 17 Jun 1993 18:57:53 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Re: Header offset GE9800 CT To: nih-image@soils.umn.edu Message-Id: <01GZHUFPSYUA000BW6@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >I suggest that you contact GE for this info since they consider it >proprietary. Years ago, I obtained this info from GE medical systems in >Milwaukee but signed a non-disclosure agreement. I've lost contact with most It is very easy to figure out the offset. First, create an image which is easy to interpret, for example, a wedge. You might be able to do this with camera controls. Then use finder get info to find out the total size of the image. The square root of this number is a maximum edge (if the image is square). Or maybe you know the dimensions. If not, you can figure them out too if you have a wedge. Just import the image with zero offset and you guess for horizontal size. If the horizontal size is wrong, then the interface From dark to bright will not be vertical. Adjust it until you get it Received: from Arizona.edu (Osprey.Telcom.Arizona.EDU) by nx1.soils.umn.edu (5.65c) id AA26097; Thu, 17 Jun 1993 18:08:05 -0500 Return-Path: Received: from pirl.lpl.arizona.edu by Arizona.edu (PMDF V4.2-11 #2381) id <01GZHOLWGHDSA4M0QM@Arizona.edu>; Thu, 17 Jun 1993 16:11:06 MST Received: by pirl.lpl.arizona.edu (4.1/SMI-3.2 LPL-MCN 1.25 91/08/23) id AA14952; Thu, 17 Jun 93 16:10:33 MST Date: Thu, 17 Jun 1993 16:10:33 -0700 (MST) From: jmorrow@pirl.lpl.arizona.edu (John Morrow) Subject: Image 1.50 Release ETA To: nih-image@soils.umn.edu Message-Id: <9306172310.AA14952@pirl.lpl.arizona.edu> Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Wayne, Is there an ETA for the next Release of NIH Image? John Morrow From tg3@u.washington.edu Thu Jun 17 09:03:33 1993 Received: from stein.u.washington.edu by nx1.soils.umn.edu (5.65c) id AA26393; Thu, 17 Jun 1993 19:00:08 -0500 Return-Path: Received: from [128.95.15.54] by stein.u.washington.edu (5.65/UW-NDC Revision: 2.22 ) id AA11043; Thu, 17 Jun 93 17:03:33 -0700 Date: Thu, 17 Jun 93 17:03:33 -0800 From: Thurman Gillespy III To: Subject: Re: Header offset GE9800 CT Cc: rowberg@u.washington.edu Message-Id: In-Reply-To: Your message <01GZHUFPSYUA000BW6@MCCLB0.MED.NYU.EDU> of Thu, 17 Jun 1993 18:00:02 -0500 Content-Type: TEXT/plain; charset=US-ASCII Figuring the offset for GE images is easy IF THE IMAGE IS NOT COMPRESSED. The raster image data follows the header, so if you know the size of your images the header (offset) size is calculated as follows: offset = filesize - (rows * columns * 2) You can find the filesize from the Get Info box in the Finder. Use the figure in parentheses. Import them in Image as unsigned 16 bit integers, Swap Bytes = OFF. For CT and MRI scans: CT: 512 * 512 * 2 = 524,288 MRI: 256 * 256 * 2 = 131,072 If the files are compressed, you're out of luck unless you have the file format and compression algorithm. If this doesn't help, contact me directly, and tell me exactly how you obtained the images and what the filenames look lile. We work with GE CT and MRI scans on our Macintoshes and workstations frequently in our Departement, and I'd be glad to help if I can. Thurman Gillespy Department of Radiology University of Washington Seattle, WA tg3@u.washington.edu From wayne@zippy.nimh.nih.gov Fri Jun 18 09:53:06 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA00934; Fri, 18 Jun 1993 09:51:44 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA02848; Fri, 18 Jun 93 11:05:42 -0400 Message-Id: <9306181505.AA02848@zippy.nimh.nih.gov> Date: Fri, 18 Jun 1993 09:53:06 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Image 1.50 Release ETA >Is there an ETA for the next Release of NIH Image? If I had to guess, I would say the official release of 1.50 will be in two or three months. One of the things I still have to do is add balloon help for all the menu commands. --wayne From SQUIDNEY@uncvx1.oit.unc.edu Fri Jun 18 08:54:10 1993 Received: from uncvx1.oit.unc.edu by nx1.soils.umn.edu (5.65c) id AA01959; Fri, 18 Jun 1993 12:47:46 -0500 Return-Path: Received: from uncvx1.oit.unc.edu by uncvx1.oit.unc.edu (PMDF V4.2-11 #3863) id <01GZIY3HQ7G0000RJW@uncvx1.oit.unc.edu>; Fri, 18 Jun 1993 13:54:10 EST Date: Fri, 18 Jun 1993 13:54:10 -0500 (EST) From: SQUIDNEY@uncvx1.oit.unc.edu Subject: Re: Fluorescence image manipulation To: nih-image@soils.umn.edu Message-Id: <01GZIY3HRJO2000RJW@uncvx1.oit.unc.edu> X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Would anyone be willing to suggest a text (reference or otherwise) on digital image processing? My specific interest is in the enhancement of fluorescence images of biological specimens but I don't wish to exclude reading in other areas. I have used Image successfully for many applications and now find that I would like to know much more about image enhancement and recovery. A general reference is sought (one which I might go to when my PI asks those sticky questions) and/or materials dealing with the use of convolutions, Fourier transforms, and the application of other digital 'filters.' I would be very happy to compile and resubmit a list if I get enough suggestions and I may be E-mailed directly at squidney@uncvx1.oit.unc.edu. Sidney L. Shaw UNC-Chapel Hill From frodes@ibg.uit.no Fri Jun 18 14:52:02 1993 Received: from benoni.Uit.No by nx1.soils.umn.edu (5.65c) id AA02040; Fri, 18 Jun 1993 12:51:05 -0500 Return-Path: Received: from benoni by ppenoni.uit.no with SMTP (PP) id <11800-0@ppenoni.uit.no>; Fri, 18 Jun 1993 19:54:23 +0000 Received: from darwin.Ibg.Uit.No by benoni.uit.no (5.65+IDA/Babel-1.15/ABaa-1.2/Ultrix) id AAbenoni11796; Fri, 18 Jun 1993 19:54:20 +0200 Received: from [129.242.200.137] (mac-if.Ibg.Uit.No) by darwin.ibg.uit.no (4.1/ABaa-2.0mini) id AA03098; Fri, 18 Jun 93 19:49:01 +0200 Message-Id: <9306181749.AA03098@darwin.ibg.uit.no> Date: Fri, 18 Jun 1993 19:52:02 -0500 To: NIH-IMAGE@soils.umn.edu From: frodes@ibg.uit.no (Frode Skarstein) X-Sender: frodes@darwin.ibg.uit.no Subject: Color analysis Hello! I am sending this mail to see if anyone of you can help me with a problem I have run into in my graduate work. I am trying to give a better evaluation of the red coloration in my charr (fish), and I want to have better values than: not red, red, very red and so on... So I was wondering if any of you knew of a program capable of analysing coloration, that is wavelenghts and intensities, for the mac. I have looked at NIH Image, but that is, as far as I know, only able to do grayscale analysis. I also have access to a copy of Adobe Photoshop, and it kind of does the job, but I am not quite pleased with it, it is more a program for picture manipulation than for analysing. So if anyone of you know about if Image can do exact evaluation of the colors in an image, or knows of any program that can, I would be very grateful for any help or hints I could get... Frode Skarstein graduate student in evolutionary ecology Department of ecology/Zoology University of Tromsoe Norway From kartenh@Sdsc.Edu Fri Jun 18 18:07:38 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA02151; Fri, 18 Jun 1993 13:04:36 -0500 Return-Path: Date: Fri, 18 Jun 93 18:07:38 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930618180738.28425877@sds.sdsc.edu> Subject: RE: Color analysis To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH to: Frode Skarstein - regarding your question of color analysis - Try ColorImage 1.32 This is a modification of Image 1.32 designed for exactly the sorts of problems you deal with. A version is available on the zippy.nimh server. The author, Joe Ayers, has completed a newer version of ColorImage that will also run on 68040 machines, but that is not yet posted. If you are using a 68030, however, you can use the version on the Server. Harvey Karten From CAZ@CU.NIH.GOV Fri Jun 18 10:41:05 1993 Received: from cu.nih.gov by nx1.soils.umn.edu (5.65c) id AA02518; Fri, 18 Jun 1993 13:40:15 -0500 Return-Path: Message-Id: <199306181840.AA02518@nx1.soils.umn.edu> To: nih-image@soils.umn.edu From: "Cindy Walczak" Date: Fri, 18 Jun 1993 14:41:05 EDT Subject: set scale question I'm having trouble making measurements with a millimeter scale in Image (using 1.47). I have a batch of xray images and an image of a ruler. I did not set the scale when I captured the images (no diamond appears after the file name in the title bar when I open the files in image). I want to set the scale to millimeters using the ruler, and have the measured lengths show up in millimeters when I do measurements on the 20 other images. BUT I cannot get the set scale to apply to anything but the ruler. Doing RECORD PREFERENCES, doesn't do it, PROPAGATE is grayed out most of the time. I know I did this before, but I have forgotten the sequence and/or trick. Can anyone out there please help? Thanks! Cindy From mvivino@helix.nih.gov Fri Jun 18 10:56:47 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA02639; Fri, 18 Jun 1993 13:53:24 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA00266; Fri, 18 Jun 93 14:56:47 -0400 Date: Fri, 18 Jun 93 14:56:47 -0400 Message-Id: <9306181856.AA00266@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: set scale question >BUT I cannot get the set scale to apply to anything but the ruler. >Doing RECORD PREFERENCES, doesn't do it, PROPAGATE is grayed out >most of the time. I know I did this before, but I have forgotten >the sequence and/or trick. Can anyone out there please help? There are several ways you can handle this 1) before capture have one image open that has your scale, then say start capturing. This image will get the scale. 2) Scale your image, load the other images and say propagate spatial scale Mark Vivino NIH/DCRT mvivino@helix.nih.gov From bsimon@pennsy.med.jhu.edu Fri Jun 18 11:38:52 1993 Received: from pennsy.med.jhu.edu by nx1.soils.umn.edu (5.65c) id AA02923; Fri, 18 Jun 1993 14:23:19 -0500 Return-Path: Received: from [128.220.90.219] by pennsy.med.jhu.edu (5.65/1.34) id AA24054; Fri, 18 Jun 93 15:38:52 -0400 Date: Fri, 18 Jun 93 15:38:52 -0400 Message-Id: <9306181938.AA24054@pennsy.med.jhu.edu> To: From: bsimon@pennsy.med.jhu.edu (Brett A. Simon) Subject: Re: set scale question >I'm having trouble making measurements with a millimeter scale >in Image (using 1.47). > >I have a batch of xray images and an image of a ruler. I did >not set the scale when I captured the images (no diamond >appears after the file name in the title bar when I open the >files in image). I want to set the scale to millimeters using >the ruler, and have the measured lengths show up in millimeters >when I do measurements on the 20 other images. > >BUT I cannot get the set scale to apply to anything but the ruler. >Doing RECORD PREFERENCES, doesn't do it, PROPAGATE is grayed out >most of the time. I know I did this before, but I have forgotten >the sequence and/or trick. Can anyone out there please help? > Thanks! > Cindy You need to 1) measure a known length of the ruler in pixels using the select line tool, 2) Use that information to set the scaling with the SET SCALE command in the ANALYZE menu, and the 3) propogate the scale to all the open images with the PROPOGATE SPARIAL CALIBRATION command. If you save your newly scaled images, the scale will be preserved. _____ Brett Simon (bsimon@pennsy.med.jhu.edu) Dept. of Anesthesia and Critical Care Medicine Johns Hopkins Hospital (410) 614-1515 From frodes@ibg.uit.no Fri Jun 18 16:35:35 1993 Received: from benoni.Uit.No by nx1.soils.umn.edu (5.65c) id AA03003; Fri, 18 Jun 1993 14:34:40 -0500 Return-Path: Received: from benoni by ppenoni.uit.no with SMTP (PP) id <12177-0@ppenoni.uit.no>; Fri, 18 Jun 1993 21:37:58 +0000 Received: from darwin.Ibg.Uit.No by benoni.uit.no (5.65+IDA/Babel-1.15/ABaa-1.2/Ultrix) id AAbenoni12173; Fri, 18 Jun 1993 21:37:53 +0200 Received: from [129.242.200.137] (mac-if.Ibg.Uit.No) by darwin.ibg.uit.no (4.1/ABaa-2.0mini) id AA03156; Fri, 18 Jun 93 21:32:34 +0200 Message-Id: <9306181932.AA03156@darwin.ibg.uit.no> Date: Fri, 18 Jun 1993 21:35:35 -0500 To: nih-image From: frodes@ibg.uit.no (Frode Skarstein) X-Sender: frodes@darwin.ibg.uit.no Subject: RE: Color analysis Thanks for responding so fast! I must ask you to give me the adress to the "zippy.nimh server" as you call it. The program you named sounds interesting, hope I can use it. frode """"""" From morgan@rock.concert.net Fri Jun 18 12:01:00 1993 Received: from rock.concert.net by nx1.soils.umn.edu (5.65c) id AA03162; Fri, 18 Jun 1993 14:57:37 -0500 Return-Path: Received: by rock.concert.net (5.59/tas-rock/8-12-92) id AA24665; Fri, 18 Jun 93 16:01:00 -0400 From: Morgan J Ryan -- Neil Patterson Publishers X-Disclaimer-1: rock is a CONCERT-CONNECT public access host. X-Disclaimer-2: Opinions expressed are not necessarily X-Disclaimer-3: those of MCNC or the CONCERT Network. Message-Id: <9306182001.AA24665@rock.concert.net> Subject: Re: Fluorescence image manipulation To: nih-image@soils.umn.edu Date: Fri, 18 Jun 93 16:01:00 EDT In-Reply-To: <01GZIY3HRJO2000RJW@uncvx1.oit.unc.edu>; from "SQUIDNEY%uncvx1.oit.unc.edu@relay.tc.umn.edu" at Jun 18, 93 12:52 pm X-Mailer: ELM [version 2.3 PL11] Hi Sidney. I edit science textbooks at a publishing house up the street from you (the old Broad Street Coffee Shop--that's us now.) I just got on the Image list and don't know much about it. I'm trying to learn everything a science editor should know about computer graphics. You sound halfway in--veteran of Image, asking for advice about what to read. Can you tell *me* what I need to read? What software I should download and fool with to learn? I'd be grateful for a little guidance. Thanks, Morgan Ryan Neil Patterson Publishers Division of Simon and Schuster 919-933-0440 morgan@rock.concert.net From wayne@zippy.nimh.nih.gov Fri Jun 18 15:24:07 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA03348; Fri, 18 Jun 1993 15:22:46 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA03434; Fri, 18 Jun 93 16:36:44 -0400 Message-Id: <9306182036.AA03434@zippy.nimh.nih.gov> Date: Fri, 18 Jun 1993 15:24:07 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Color analysis Image can capture 24-bit color using either the Capture Color or Acquire commands. The color image is stored as a three slice(red, green and blue) stack. To measure red coloration all you have do is measure mean intensities in the red slice. In the near future, I plan to add the ability to convert from RGB to HSI(hue, saturation, intensity), which will allow intensities of color images to be measured and also allow thresolding based on hue. --wayne From wching@alice.music.yale.edu Fri Jun 18 12:51:33 1993 Received: from ALICE.MUSIC.YALE.EDU by nx1.soils.umn.edu (5.65c) id AA03499; Fri, 18 Jun 1993 15:48:11 -0500 Return-Path: Received: by alice.music.yale.edu (NX5.67c/NX3.0M) id AA03923; Fri, 18 Jun 93 16:51:33 -0400 Date: Fri, 18 Jun 93 16:51:33 -0400 From: William Ching Message-Id: <9306182051.AA03923@alice.music.yale.edu> To: nih-image@soils.umn.edu Reply-To: ching-william@yale.edu statistics nih-image From gmei@hoh.mbl.edu Fri Jun 18 12:53:26 1993 Received: from AQUA.WHOI.EDU by nx1.soils.umn.edu (5.65c) id AA03612; Fri, 18 Jun 1993 15:55:31 -0500 Return-Path: Received: by aqua.whoi.edu (5.57/Ultrix3.0-C) id AA28838; Fri, 18 Jun 93 16:58:54 -0400 Received: from [128.128.172.222] (STARMAC22.MBL.EDU) by hoh.mbl.edu. (4.1/SMI-4.1) id AA10233; Fri, 18 Jun 93 16:53:27 EDT Date: Fri, 18 Jun 93 16:53:26 EDT Message-Id: <9306182053.AA10233@hoh.mbl.edu.> To: nih-image@soils.umn.edu From: gmei@hoh.mbl.edu (Guang Mei) Subject: Remote Focus Accessory controlled by Mac? Hi, Does anyone have experience using a Mac to control "Remote Focus Accessory" made by Nikon? We have a Nikon Microphot-SA type microscope, there is a Remote Focus Accessory (a small box) which is connected to the microscope to control the stage (to do z-scan). In the booklet it said that Remote Focus Accessory can be controlled by IBM PC/XT type computer through RS232. however when I tried to use Mac IIx to control the Remote Focus Accessory, I did not get any response (I used Modem port on the Mac). I am wondering whether this is doable or not. Any response is appreciated. ------------- Guang Mei gmei@hoh.mbl.edu Tel:(508)548-3705 ext 374 Program of Architectural Dynamics in Living Cells Marine Biological Laboratory, Woods Hole, MA 02543-1031 From ltague@physio1.utmem.edu Fri Jun 18 11:17:28 1993 Received: from physio1.utmem.edu by nx1.soils.umn.edu (5.65c) id AA03739; Fri, 18 Jun 1993 16:13:50 -0500 Return-Path: Received: by physio1.utmem.edu (5.57/Ultrix3.0-C) id AA14853; Fri, 18 Jun 93 16:17:28 -0500 Message-Id: <9306182117.AA14853@physio1.utmem.edu> To: Cc: Multiple recipients of list , ltague@physio1.utmem.edu Subject: Re: Remote Focus Accessory controlled by Mac? In-Reply-To: Your message of "Fri, 18 Jun 93 15:56:14 CDT." <9306182053.AA10233@hoh.mbl.edu.> Date: Fri, 18 Jun 93 16:17:28 -0500 From: ltague@physio1.utmem.edu X-Mts: smtp >Remote Focus Accessory (a small box) which is connected to the microscope >to control the stage (to do z-scan). In the booklet it said that Remote >Focus Accessory can be controlled by IBM PC/XT type computer through RS232. >however when I tried to use Mac IIx to control the Remote Focus Accessory, >I did not get any response (I used Modem port on the Mac). I am wondering >whether this is doable or not. Any response is appreciated. ------------- >Guang Mei gmei@hoh.mbl.edu Tel:(508)548-3705 ext 374 >Program of Architectural Dynamics in Living Cells >Marine Biological Laboratory, Woods Hole, MA 02543-1031 If you know which pins on the RS232 actually control the serial device you should have little problem (?????) configuring the MacII to accomplish the same task. Make sure that you are talking at the right speed. Some of the "semi-intelligent" instruments are sensitive to the communication specifications set on the Mac side. Also an in-line RS232 "snooper" can be very useful. Larry From RBLYSTON@VM1.TUCC.TRINITY.EDU Fri Jun 18 11:34:49 1993 Received: from vm1.tucc.trinity.edu by nx1.soils.umn.edu (5.65c) id AA03993; Fri, 18 Jun 1993 16:34:32 -0500 Return-Path: Message-Id: <199306182134.AA03993@nx1.soils.umn.edu> Received: from VM1.tucc.trinity.edu by VM1.TUCC.TRINITY.EDU (IBM VM SMTP V2R1) with BSMTP id 9632; Fri, 18 Jun 93 16:37:11 CDT Received: from TRINITY.EDU (RBLYSTON) by VM1.tucc.trinity.edu (Mailer R2.10 ptf000) with BSMTP id 6767; Fri, 18 Jun 93 16:37:07 CDT Date: Fri, 18 Jun 93 16:34:49 CDT From: Robert Blystone Subject: Re: Remote Focus Accessory controlled by Mac? To: nih-image@soils.umn.edu In-Reply-To: Message of Fri, 18 Jun 1993 15:59:42 -0500 from We use the Nikon Remote Focus Accessory regularly; however, we run it on an old XT that was going to be used as a door stop. To my knowledge there is no Mac version of the software. You can probably find an old XT for virtually nothing and just use the program provided by Nikon. It works well. Blystone in Texas ********************** ROBERT V. BLYSTONE PHONE:(210)736-7243 DEPARTMENT OF BIOLOGY FAX:(210)736-7229 Trinity University E-Mail:RBlyston@Trinity.edu 715 Stadium Drive San Antonio, TX, 78212 From gstuart@unixg.ubc.ca Fri Jun 18 08:08:29 1993 Received: from unixg.ubc.ca by nx1.soils.umn.edu (5.65c) id AA04962; Fri, 18 Jun 1993 17:16:45 -0500 Return-Path: Received: by unixg.ubc.ca (4.1/1.14) id AA00709; Fri, 18 Jun 93 15:20:08 PDT Date: Fri, 18 Jun 1993 15:08:29 -0700 (PDT) From: Gregory Stuart Subject: comet (single cell gel) software To: nih-image@soils.umn.edu Cc: Multiple recipients of list Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hello! Perhaps the subscribers to this list could help me. I am a graduate student, and our lab has recently purchased the Perceptics pixelbuffer board and a Cohu CCD camera to do image analysis on the Mac. We are using Image144VDM supplied by Perceptics. We want to use the system to monitor for genetic damage in occupational settings. Single human cells are spread on a microscope slide in agarose, the cells are lysed, and the DNA is electrophoresed. DNA which has been damaged fragments and runs ahead of the main body of the DNA. The DNA is stained with a fluorescent dye and the image captured through a microscope for quantitation. This technique is variously called the "single-cell gel electrophoresis" or "comet" assay (the product looks like a comet with "head" and "tail" regions). We want to separately quantitate the head and tail regions. QUESTION : commercial software has been written, but we are a new lab on a very limited budget. Is there any public-domain software out there written for this purpose? Any help would be greatly appreciated! Thanks in advance - Greg Stuart. From salzman@Athena.MIT.EDU Sun Jun 20 10:36:15 1993 Received: from ATHENA.MIT.EDU by nx1.soils.umn.edu (5.65c) id AA14449; Sun, 20 Jun 1993 13:34:12 -0500 Return-Path: Received: from CASCADE.MIT.EDU by Athena.MIT.EDU with SMTP id AA01139; Sun, 20 Jun 93 14:37:32 EDT From: salzman@Athena.MIT.EDU Received: by cascade.MIT.EDU (5.57/4.7) id AA00931; Sun, 20 Jun 93 14:36:15 -0400 Date: Sun, 20 Jun 93 14:36:15 -0400 Message-Id: <9306201836.AA00931@cascade.MIT.EDU> To: nih-image@soils.umn.edu Subject: Radius Rocket 33 We looked closely at the Rocket, and ended up not being able to keep it working. Radius makes excellent products and has some of the best technical support practices and people in the industry, but the Rocket is not entirely stable and probably never will be. Much of the nominal speed advantage is lost in practice to bus arbitration, even though the Rocket uses block mode transfers; mysterious crashes kept crashing the IIcx we tried to accelerate, which the Rocket's principal engineer attributed to "errors in Apple's 32-bit compatibility code." From salzman@Athena.MIT.EDU Sun Jun 20 10:45:17 1993 Received: from ATHENA.MIT.EDU by nx1.soils.umn.edu (5.65c) id AA14596; Sun, 20 Jun 1993 13:43:14 -0500 Return-Path: Received: from CASCADE.MIT.EDU by Athena.MIT.EDU with SMTP id AA01286; Sun, 20 Jun 93 14:46:34 EDT From: salzman@Athena.MIT.EDU Received: by cascade.MIT.EDU (5.57/4.7) id AA01000; Sun, 20 Jun 93 14:45:17 -0400 Date: Sun, 20 Jun 93 14:45:17 -0400 Message-Id: <9306201845.AA01000@cascade.MIT.EDU> To: nih-image@soils.umn.edu Subject: Textbooks on Image Processing Two classics in the field are by Harry Andrews: Andrews & Pratt and Andrews & Hunt (or Pratt & Andrews, etc). The books present the state of the art circa 1970, which encompasses most of Fourier optics and digital image reconstruction. There are a number of more recent works, but none is as succinct as these. For a comprehemsive treatment of optics, but not of digital optics or non-linear optics, see Born & Wolf. Hope this helps. - David Salzman, Ph.D. From mhaury@pasteur.fr Mon Jun 21 11:58:26 1993 Received: from mendel.sis.pasteur.fr by nx1.soils.umn.edu (5.65c) id AA18297; Mon, 21 Jun 1993 02:55:09 -0500 Return-Path: Received: from [157.99.132.91] by mendel.sis.pasteur.fr with SMTP id AA01809 (5.65c8/IDA-1.4.4 for nih-image@soils.umn.edu); Mon, 21 Jun 1993 09:58:26 +0200 Date: Mon, 21 Jun 1993 09:58:26 +0200 Message-Id: <199306210758.AA01809@mendel.sis.pasteur.fr> To: nih-image@soils.umn.edu From: mhaury@pasteur.fr (Matthias Haury) X-Sender: mhaury@pasteur.fr (Unverified) Subject: Plot Profile Macro for Multiple Lanes I would like to ask everybody out there if you have heard about a macro that allows to define automatically several lanes on a image (we have westernblots or coomassie blue gel images and would like to scan the plotprofiles of 28 lanes on each gel) and export the plot profile in a (or multiple) text file(s). What we would like, is to have a procedure to p.ex. define the first and the last lane on the image, and have the macro find the other lanes (the user enters the number of total lanes) automatically, and then export all the plot profiles in a (preferably one) text file. Every type of macro that does (even part of the job p.ex. export routines etc..) would be greatly appreciated. Thanks a lot... Matthias ------------------------ Matthias Haury Institut Pasteur 25 rue du Doctor Roux 75724 PARIS CEDEX 15 Tel: (33)-1-45.68.84.21 Fax: (33)-1-45.68.86.39 EMail: mhaury@pasteur.fr From mvivino@helix.nih.gov Mon Jun 21 04:43:22 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA19530; Mon, 21 Jun 1993 07:40:04 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA13340; Mon, 21 Jun 93 08:43:22 -0400 Date: Mon, 21 Jun 93 08:43:22 -0400 Message-Id: <9306211243.AA13340@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: Plot Profile Macro for Multiple Lanes >I would like to ask everybody out there if you have heard about a macro >that allows to define automatically several lanes on a image (we have >westernblots or coomassie blue gel images and would like to scan the >plotprofiles of 28 lanes on each gel) and export the plot profile in a (or >multiple) text file(s). What we would like, is to have a procedure to p.ex. >define the first and the last lane on the image, and have the macro find >the other lanes (the user enters the number of total lanes) automatically, >and then export all the plot profiles in a (preferably one) text file. You have to answer several questions for yourself on this one before you start. Are your lanes exactly evenly spaced? If not then you have a difficult task at best. If so, you can easily have a ROI which moves across the image in a loop. Inside the loop it will do a plot profile and export the plot profile. It should only ask for the filename of the first one, then it can concat the index of the loop for a filename and should not ask on the rest. You start your macro by reading in the roi which you have drawn into several vars. It is easy to increment the var to the next lane if you know how many pixels to the next lane. A MakeROI call can create the ROI. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From mvivino@helix.nih.gov Mon Jun 21 04:46:22 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA19569; Mon, 21 Jun 1993 07:43:04 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA13791; Mon, 21 Jun 93 08:46:22 -0400 Date: Mon, 21 Jun 93 08:46:22 -0400 Message-Id: <9306211246.AA13791@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: Radius Rocket 33 In our lab we have purchased several accelerators over the past year. One a radius rocket (68040) and one a daystar (68030). The daystar has worked flawlessly since the day installed and has never crashed. The radius rocket does not last 5 minutes in any computer that it is installed. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From ruzin@nature.berkeley.edu Mon Jun 21 00:19:21 1993 Received: from nettlerash.Berkeley.EDU by nx1.soils.umn.edu (5.65c) id AA20454; Mon, 21 Jun 1993 10:12:50 -0500 Return-Path: Received: from nature.Berkeley.EDU by nettlerash.berkeley.edu (5.67.1/1.34.6) id AA12153; Mon, 21 Jun 93 08:14:29 -0700 Received: from [128.32.128.167] (kos5mac12.Berkeley.EDU) by nature.berkeley.edu.cnr-net (4.1/SMI-4.1) id AA02257; Mon, 21 Jun 93 08:16:55 PDT Message-Id: <9306211516.AA02257@nature.berkeley.edu.cnr-net> Date: Mon, 21 Jun 1993 08:19:21 -0800 To: From: ruzin@nature.berkeley.edu (Steven Ruzin) Subject: Re: Textbooks on Image Processing >Two classics in the field are by Harry Andrews: Andrews & Pratt and >Andrews & Hunt (or Pratt & Andrews, etc). The books present the state >of the art circa 1970, which encompasses most of Fourier optics and >digital image reconstruction. There are a number of more recent works, >but none is as succinct as these. >For a comprehemsive treatment of optics, but not of digital optics >or non-linear optics, see Born & Wolf. > >Hope this helps. > It would if we had the entire reference. ie: Title, publisher... Steven Ruzin NSF Center of Plant Developmental Biology Dept Plant Biology Univ California Berkeley From provost@corl.nbc.upenn.edu Mon Jun 21 07:17:31 1993 Received: from CORL.NBC.UPENN.EDU by nx1.soils.umn.edu (5.65c) id AA20512; Mon, 21 Jun 1993 10:17:12 -0500 Return-Path: Received: by corl.nbc.upenn.edu (921113.SGI.UNSUPPORTED_PROTOTYPE/921111.SGI.ANONFTP) for nih-image@soils.umn.edu id AA02377; Mon, 21 Jun 93 11:17:31 -0400 Date: Mon, 21 Jun 93 11:17:31 -0400 From: provost@corl.nbc.upenn.edu (Michael T. Provost) Message-Id: <9306211517.AA02377@corl.nbc.upenn.edu> To: nih-image@soils.umn.edu Our lab is contemplating the purchase of a color (RGB) camera. The primary application would be for digitizing fluorochrome labelled thick (~100um) cross-sections of equine cortical bone illuminated in reflected light under a microscope. Labels are red, orange, yellow, green and blue. Except for the blue label, all of the labels are very, very bright - this is not a low light application. Currently, we have a Quadra 700, a DT Quick Capture board and a Pulnix TM-745 black and white camera. Minimally, our wish list includes: 640 x 480 resolution, low noise, gain control (I assume this applies to color as it does in black and white?) and a price tag that won't break the bank. I would appreciate the comments, thoughts, experiences and recommendations of others with respect to specific RGB cameras and their use with NIH-Image. Please reply to me directly - provost@corl.nbc.upenn.edu I will summarize and post the results of this inquiry in a few weeks - if there is anything to summarize and post :-). Thanks for your help, Mike ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Michael T. Provost, VMD Comparative Orthopaedic Research Laboratory Phone: 215-444-5800x2267 U of Pennsylvania School of Veterinary Medicine FAX: 215-444-4724 Kennett Square, PA 19348-1692 provost@corl.nbc.upenn.edu From ruzin@nature.berkeley.edu Mon Jun 21 00:26:54 1993 Received: from nettlerash.Berkeley.EDU by nx1.soils.umn.edu (5.65c) id AA20566; Mon, 21 Jun 1993 10:20:23 -0500 Return-Path: Received: from nature.Berkeley.EDU by nettlerash.berkeley.edu (5.67.1/1.34.6) id AA12230; Mon, 21 Jun 93 08:22:03 -0700 Received: from [128.32.128.167] (kos5mac12.Berkeley.EDU) by nature.berkeley.edu.cnr-net (4.1/SMI-4.1) id AA03163; Mon, 21 Jun 93 08:24:28 PDT Message-Id: <9306211524.AA03163@nature.berkeley.edu.cnr-net> Date: Mon, 21 Jun 1993 08:26:54 -0800 To: From: ruzin@nature.berkeley.edu (Steven Ruzin) Subject: Re: Textbooks on Image Processing >Two classics in the field are by Harry Andrews: Andrews & Pratt and >Andrews & Hunt (or Pratt & Andrews, etc). The books present the state >of the art circa 1970, which encompasses most of Fourier optics and >digital image reconstruction. There are a number of more recent works, >but none is as succinct as these. >For a comprehemsive treatment of optics, but not of digital optics >or non-linear optics, see Born & Wolf. > A couple of very good contemporary references on Image Processing are: 1) Russ, JC. 1992. The Image Processing handbook. CRC Press. This is a good basic introduction to the subject. It doesn't go into detail, but it's a good place to start. 2) Gonzalez, RC and RE Woods. 1992. Digital image processing. Addison-Wesley Publishing Company. A much more thorough coverage of the subject. Includes Fourier transforms, image compression, and a bit on pattern recognition and expert systems. Of course one of the most thorough treatises on microscope optics and video microscopy is: 3) Inoue, S. 1986. Video Microscopy. Plenum Press. Steve... Steven Ruzin NSF Center of Plant Developmental Biology Dept Plant Biology Univ California Berkeley From mark@hephaestus.earth.monash.edu.au Tue Jun 22 04:28:01 1993 Received: from monu1.cc.monash.edu.au by nx1.soils.umn.edu (5.65c) id AA23302; Mon, 21 Jun 1993 18:25:29 -0500 Return-Path: Received: from hephaestus.earth.monash.edu.au by monu1.cc.monash.edu.au (5.61/1.34) id AA00209; Tue, 22 Jun 93 09:28:41 +1000 Received: from [130.194.168.11] (mark) by hephaestus.earth.monash.edu.au (4.1/SMI-4.1) id AA01073; Tue, 22 Jun 93 09:28:02 EST Date: Tue, 22 Jun 93 09:28:01 EST Message-Id: <9306212328.AA01073@hephaestus.earth.monash.edu.au> To: From: mark@hephaestus.earth.monash.edu.au (Mark Jessell) Subject: Re: Textbooks on Image Processing Another good textbook is Computer-Assisted Microscopy by John C Russ, 1990 Plenum Press, New York. ISBN 0-306-43410-5 Mark Mark Jessell Victorian Institute of Earth & Planetary Sciences Dept of Earth Sciences Monash University, Clayton, VIC, 3168 Australia mark@hephaestus.earth.monash.edu.au Tel (61)(3) 565 4902 Fax (61)(3) 565 4903 From SBK111@SBMS01.MSRC.SUNYSB.EDU Mon Jun 21 15:41:00 1993 Received: from sbms01.msrc.sunysb.edu by nx1.soils.umn.edu (5.65c) id AA23414; Mon, 21 Jun 1993 18:38:14 -0500 Return-Path: Date: Mon, 21 Jun 93 19:41 EDT From: Barr Kum Subject: Re: NTSC video from the Mac? To: nih-image@soils.umn.Edu Message-Id: <1A0B7A2E2D5F80B680@SBMS01.MSRC.SUNYSB.EDU> X-Organization: Marine Sciences Research Center X-Envelope-To: nih-image@soils.umn.Edu X-Vms-To: IN%"nih-image@soils.umn.Edu" X-Vms-Cc: SBK111 Sometime back I posted a query requesting help on recording a single image at a time onto a VCR. I did recieve replies which are appended at the end. I did manage to make a movie sequence by recording each image in this manner. This method bypasses the memory constraint when using animations on the computer. Equipment used was 1) Mac II cx 2) Syscom Mediator Scan convertor 3) Nec PC VCR (serial port controllable) Software NIH Image 1.49 Multiple images of contour plots were made on a workstation saved in Tiff format and telneted onto the Mac. Caution if you can generate PICT files use them as they use up less space. Out of the 600 images generated there was space for only 250 on a disk with 80 MB free space. The VCR was hooked up to the video output via scan convertor. Another line connecting VCR serial port and Mac serial was used to control VCR actions from the Mac. A macro written in NIH image was then used to crontrol the action of the VCR. Each image w called up. The menu bar etc were visible in the movie. Wayne Rasband has said that he will consider having a Macro call for PHOTOMODE. This should solve that problem. For some reason if a Pause command was sent to the VCR insted of a STOP the syncronisation got mixed up. Recording would occur when the image was being closed etc. Ive given the Macro used below. The general outline of the macro is as follows 1) Call up a image file 2) Send message to VCR via serial port to start recording 3) Time a sec (or any other) 4) Send message to VCR to stop recording 5) close file, loop What is needed is to have photomode between 1 and 2, where the whole Image fills the screen. I had to do this thru a macro as i was sending 250 images to the VCR. However f you are sending only a couple you could load the image, go into photomode and have a macro that will can be activated by command keys to send message to VCR to start recording and stop after fixed time. Hope this helps Sereno Barr-Kumarakulasinghe Marine Sciences Research Center SUNY @ Stony Brook NY 11794-5000 e-mail: sbarrkum@csserv1.ic.sunysb.edu sbk111@msrc.sunysb.edu ------------------------------------------------------------------ macro 'FileToVCR'; var linefeed,return,r,c,p,s,t,crlf,rc, ps,st,file:string; i:integer; begin r:=chr(82); c:=chr(67); p:=chr(80); s:=chr(83); t:=chr(84); linefeed:=chr(10); return:=chr(13); crlf:=concat(return,linefeed); rc:=concat(r,c,crlf); ps:=concat(p,s,crlf) st:=concat(s,t,crlf) RequiresVersion(1.48); OpenSerial('1200 baud,no parity,eight data,one stop'); for i:=1 to 275 do begin SetImport('TIFF'); Import('Temt', i:3) {photo Mode should be included here when available} PutSerial(rc); Wait(4); PutSerial(st); Wait(3);} close; Beep; end end; ----------------------------------------------------------- From: mvivino@helix.nih.gov Barr Kum writes: >I need to know of a way to write images from a window to a videotape on a >VCR. We've got a NEC VCR that is supposed to be capable of advancing frame >by frame. You have a number of options, depending on your budget and requirements you might be able to do the job with next to nothing, or you might need fairly big bucks. Option 1) Get access to a quadra. The quadra has built in video. You will need to make or buy a cable that goes from the quadra to BNC. Read trhe document: quadra_video.txt in /pub/image/documents of zippy.nimh.nih.gov. I might also be able to send you a schematic. YOU ****MUST**** USE APPLE CONVOLUTION MODE in the monitors control panal to get decent video. You will only get grayscale. Option 2) (not recommended) Use a RasterOps video expander. Its cheap (and its also inexpensive). It does a worse job than the quadra video. Option 3) Buy a radius video vision, get color and decent video. High cost. For all the above options you can use the serial port macro calls to advance your vcr, if the vcr is that "smart" a vcr. Mark ---------------------------------------------------------------------------- From: kartenh@sdsc.edu Another option is the Scion NTSC output card, for about $595.?[ --------------------------------------------------------------------------- From: D1887@AppleLink.Apple.Com FROM: Bonnie Polesky D1887 SUB: Mac to VCR Dear Mr. Kum: In response to your inquiry of writing images from a Mac window to a VCR, Scion Corporation offers a product, the TV-3, which is capable of outputing grayscale images from a version of NIH Image in just this way. The TV-3 converts the image contained in its memory buffer into an RS-170 video signal for outputting to devices such as video tape recorders and video printers. The TV-3 sells for $595 and is available direct from Scion. If you wish to receive literature on the TV-3, please forward your address and fax number to us. Please feel free to contact us if you have any questions. Sincerely, Bonnie Polesky Director, Sales & Marketing =============================================================================== From: IN%"mvivino@helix.nih.Gov" 8-APR-1993 15:18:16.84 Subj: RE: Mac to VCR (using a quadra 950) >From what youve said and going thru the document quadra..at zippy... it looks >like color output can be written onto video-tape. However how do I get about >sending only what is in a window to the vcr as against the whole screen. >Any advice really welcome. I was pretty sure it was only grayscale. What you need to do is first make the cable. I have uploaded a schematic to zippy.nimh.nih.gov in the pub/image/contrib directory. Then to output just the image you can do one of two things: 1) Have only the cable running as video and load the image into the Image program. Then select Photomode. 2) Use two monitors, one from the cable the other from a built in video card (use this as monitor one). Then make your image scaled as large as monitor two. Mark ============================================================================ From SBK111@SBMS01.MSRC.SUNYSB.EDU Mon Jun 21 15:45:00 1993 Received: from sbms01.msrc.sunysb.edu by nx1.soils.umn.edu (5.65c) id AA23446; Mon, 21 Jun 1993 18:42:33 -0500 Return-Path: Date: Mon, 21 Jun 93 19:45 EDT From: Barr Kum Subject: Re: MAC to VCR (Sorry for the delay) To: nih-image@soils.umn.Edu Message-Id: <1A0ADE1E16FF80B680@SBMS01.MSRC.SUNYSB.EDU> X-Organization: Marine Sciences Research Center X-Envelope-To: nih-image@soils.umn.Edu X-Vms-To: IN%"nih-image@soils.umn.edu" X-Vms-Cc: SBK111 Sorry for the delay in re-posting this message. Hope it helps. Barr-Kum --------------------------------------------- Sometime back I posted a query requesting help on recording a single image at a time onto a VCR. I did recieve replies which are appended at the end. I did manage to make a movie sequence by recording each image in this manner. This method bypasses the memory constraint when using animations on the computer. Equipment used was 1) Mac II cx 2) Syscom Mediator Scan convertor 3) Nec PC VCR (serial port controllable) Software NIH Image 1.49 Multiple images of contour plots were made on a workstation saved in Tiff format and telneted onto the Mac. Caution if you can generate PICT files use them as they use up less space. Out of the 600 images generated there was space for only 250 on a disk with 80 MB free space. The VCR was hooked up to the video output via scan convertor. Another line connecting VCR serial port and Mac serial was used to control VCR actions from the Mac. A macro written in NIH image was then used to crontrol the action of the VCR. Each image w called up. The menu bar etc were visible in the movie. Wayne Rasband has said that he will consider having a Macro call for PHOTOMODE. This should solve that problem. For some reason if a Pause command was sent to the VCR insted of a STOP the syncronisation got mixed up. Recording would occur when the image was being closed etc. Ive given the Macro used below. The general outline of the macro is as follows 1) Call up a image file 2) Send message to VCR via serial port to start recording 3) Time a sec (or any other) 4) Send message to VCR to stop recording 5) close file, loop What is needed is to have photomode between 1 and 2, where the whole Image fills the screen. I had to do this thru a macro as i was sending 250 images to the VCR. However f you are sending only a couple you could load the image, go into photomode and have a macro that will can be activated by command keys to send message to VCR to start recording and stop after fixed time. Hope this helps Sereno Barr-Kumarakulasinghe Marine Sciences Research Center SUNY @ Stony Brook NY 11794-5000 e-mail: sbarrkum@csserv1.ic.sunysb.edu sbk111@msrc.sunysb.edu ------------------------------------------------------------------ macro 'FileToVCR'; var linefeed,return,r,c,p,s,t,crlf,rc, ps,st,file:string; i:integer; begin r:=chr(82); c:=chr(67); p:=chr(80); s:=chr(83); t:=chr(84); linefeed:=chr(10); return:=chr(13); crlf:=concat(return,linefeed); rc:=concat(r,c,crlf); ps:=concat(p,s,crlf) st:=concat(s,t,crlf) RequiresVersion(1.48); OpenSerial('1200 baud,no parity,eight data,one stop'); for i:=1 to 275 do begin SetImport('TIFF'); Import('Temt', i:3) {photo Mode should be included here when available} PutSerial(rc); Wait(4); PutSerial(st); Wait(3);} close; Beep; end end; ----------------------------------------------------------- From: mvivino@helix.nih.gov Barr Kum writes: >I need to know of a way to write images from a window to a videotape on a >VCR. We've got a NEC VCR that is supposed to be capable of advancing frame >by frame. You have a number of options, depending on your budget and requirements you might be able to do the job with next to nothing, or you might need fairly big bucks. Option 1) Get access to a quadra. The quadra has built in video. You will need to make or buy a cable that goes from the quadra to BNC. Read trhe document: quadra_video.txt in /pub/image/documents of zippy.nimh.nih.gov. I might also be able to send you a schematic. YOU ****MUST**** USE APPLE CONVOLUTION MODE in the monitors control panal to get decent video. You will only get grayscale. Option 2) (not recommended) Use a RasterOps video expander. Its cheap (and its also inexpensive). It does a worse job than the quadra video. Option 3) Buy a radius video vision, get color and decent video. High cost. For all the above options you can use the serial port macro calls to advance your vcr, if the vcr is that "smart" a vcr. Mark ---------------------------------------------------------------------------- From: kartenh@sdsc.edu Another option is the Scion NTSC output card, for about $595.?[ --------------------------------------------------------------------------- From: D1887@AppleLink.Apple.Com FROM: Bonnie Polesky D1887 SUB: Mac to VCR Dear Mr. Kum: In response to your inquiry of writing images from a Mac window to a VCR, Scion Corporation offers a product, the TV-3, which is capable of outputing grayscale images from a version of NIH Image in just this way. The TV-3 converts the image contained in its memory buffer into an RS-170 video signal for outputting to devices such as video tape recorders and video printers. The TV-3 sells for $595 and is available direct from Scion. If you wish to receive literature on the TV-3, please forward your address and fax number to us. Please feel free to contact us if you have any questions. Sincerely, Bonnie Polesky Director, Sales & Marketing =============================================================================== From: IN%"mvivino@helix.nih.Gov" 8-APR-1993 15:18:16.84 Subj: RE: Mac to VCR (using a quadra 950) >From what youve said and going thru the document quadra..at zippy... it looks >like color output can be written onto video-tape. However how do I get about >sending only what is in a window to the vcr as against the whole screen. >Any advice really welcome. I was pretty sure it was only grayscale. What you need to do is first make the cable. I have uploaded a schematic to zippy.nimh.nih.gov in the pub/image/contrib directory. Then to output just the image you can do one of two things: 1) Have only the cable running as video and load the image into the Image program. Then select Photomode. 2) Use two monitors, one from the cable the other from a built in video card (use this as monitor one). Then make your image scaled as large as monitor two. Mark ============================================================================ From mvivino@helix.nih.gov Tue Jun 22 05:20:16 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA27011; Tue, 22 Jun 1993 08:16:58 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA23439; Tue, 22 Jun 93 09:20:16 -0400 Date: Tue, 22 Jun 93 09:20:16 -0400 Message-Id: <9306221320.AA23439@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Image text window for typing comments & results For Image 1.50b Since many people want a window for typing comments, as well as placement of data or results, perhaps they can now use the new text window and copy and paste from the results window. It does seem to work. Be sure to check headings in the Options... (under Analyze) to include the heading from the last measurement. Then paste into the text window and add your comments. Haven't tried doing this with a macro yet, but I don't see why not. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From wayne@zippy.nimh.nih.gov Tue Jun 22 09:08:33 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA27462; Tue, 22 Jun 1993 09:07:19 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA04438; Tue, 22 Jun 93 09:22:50 -0400 Message-Id: <9306221322.AA04438@zippy.nimh.nih.gov> Date: Tue, 22 Jun 1993 09:08:33 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: MAC to VCR with Photomode command Here is a version Barr-Kum's macro for recording a sequence of images on a NEC PC VCR that uses the new Photomode command in V1.50. I had to add the SetCursor command to get the cursor to reappear. --wayne ----------------------------------------------------------- macro 'Mac To NEC PC VCR'; var linefeed,return,r,c,p,s,t,crlf,rc, ps,st,file:string; i:integer; begin r:=chr(82); c:=chr(67); p:=chr(80); s:=chr(83); t:=chr(84); linefeed:=chr(10); return:=chr(13); crlf:=concat(return,linefeed); rc:=concat(r,c,crlf); ps:=concat(p,s,crlf) st:=concat(s,t,crlf) RequiresVersion(1.50); OpenSerial('1200 baud,no parity,eight data,one stop'); for i:=1 to 275 do begin SetImport('TIFF'); Import('Temp', i:3) Photomode(true); PutSerial(rc); Wait(4); PutSerial(st); Wait(3); Photomode(false); SetCursor('Arrow'); close; Beep; end end; From LOBSTER@northeastern.edu Sat Jun 22 06:28:32 1993 Received: from NUHUB.CRC.NORTHEASTERN.EDU (nuhub.dac.northeastern.edu) by nx1.soils.umn.edu (5.65c) id AA27961; Tue, 22 Jun 1993 10:25:29 -0500 Return-Path: Received: from northeastern.edu by northeastern.edu (PMDF #3067 ) id <01GZOE2L381S9D6BYT@northeastern.edu>; Tue, 22 Jun 1993 11:28:32 EST Date: 22 Jun 1993 11:28:32 -0500 (EST) From: LOBSTER@northeastern.edu Subject: MacWorld Expo To: nih-image@soils.umn.edu Message-Id: <01GZOE2L4AMQ9D6BYT@northeastern.edu> X-Envelope-To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Transfer-Encoding: 7BIT I am organizing a session of the Education Conference at MacWorld Espo in Boston titled "Cutting Edge Scientific Applications of the Macintosh" and would like to get someone to talk about applications of the new features of NIH Image. Wayne isn't attending so If you have any ideas contact me at (617) 581-7370. I need to organize this ASAP Thanks Joseph Ayers Internet: lobster@northeastern.edu Applelink: U0729 From mvivino@helix.nih.gov Tue Jun 22 09:18:42 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA28626; Tue, 22 Jun 1993 12:15:26 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA29457; Tue, 22 Jun 93 13:18:42 -0400 Date: Tue, 22 Jun 93 13:18:42 -0400 Message-Id: <9306221718.AA29457@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Software for volume render of non-aligned 3D pixel data? CC to sci.image.processing, comp.graphics I'm looking for software which will accept the X, Y, Z and pixel value for each point in a 3-Dimensional volume space (i.e. Pi(Xi, Yi, Zi )). These points are not symmetrically spaced with respect to each other. The input file might look something like: X Y Z Value 21.836 14.227 5.501 18 22.919 13.745 4.90 31 etc. etc. The data is therefore in the form of cross-sections through the 3-D volume, taken at different known orientations. The cross-sections are not parallel to each other. The data points are fairly sparse, especially between the cross-sections. I'd like to find software which will take this input and generate a 3-D Cartesian filled volume of data points (symmetrically spaced filled volume). Since the unmeasured (missing) data points are numerous, and with different number of missing points in each direction, the interpolation should be made with different weights and window sizes in each direction. So what software is out there that has this volume generating capability? Additionally, if you have similar type data and have found any pointers of use, I'd like to hear those too. Thanks in advance, Mark Vivino NIH/DCRT mvivino@helix.nih.gov From kartenh@Sdsc.Edu Tue Jun 22 17:38:25 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA28911; Tue, 22 Jun 1993 12:35:27 -0500 Return-Path: Date: Tue, 22 Jun 93 17:38:25 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930622173825.2880213c@sds.sdsc.edu> Subject: RE: Image text window for typing comments & results To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Mark, The text window works very well. Is there also a way to paste in graphics data (e.g. Profile of a Line Plot) to the text window? If I copy a plot and try ot paste it into the text window, instead it pastes it into the original grpahics graphics [sic] window. Also, is there a way to copy only selected columns from the Results Window? ( i.e. without first resetting the columns under the Options window). Score another big point for Wayne. regards, Harvey From kartenh@Sdsc.Edu Tue Jun 22 17:40:31 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA28947; Tue, 22 Jun 1993 12:37:19 -0500 Return-Path: Date: Tue, 22 Jun 93 17:40:31 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930622174031.2880213c@sds.sdsc.edu> Subject: Undo function in Text Window? To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" Wayne, Will there be an undo function in the text window? Can the Text window be opened with a Macro? regards, Harvey From wayne@zippy.nimh.nih.gov Tue Jun 22 12:46:35 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA29035; Tue, 22 Jun 1993 12:45:21 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA04934; Tue, 22 Jun 93 13:00:53 -0400 Message-Id: <9306221700.AA04934@zippy.nimh.nih.gov> Date: Tue, 22 Jun 1993 12:46:35 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Undo function in Text Window? >Wayne, > Will there be an undo function in the text window? >Can the Text window be opened with a Macro? These are both on my list of things to do. --wayne From mvivino@helix.nih.gov Tue Jun 22 10:01:32 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA29150; Tue, 22 Jun 1993 12:58:25 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA05940; Tue, 22 Jun 93 14:01:32 -0400 Date: Tue, 22 Jun 93 14:01:32 -0400 Message-Id: <9306221801.AA05940@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: RE: Image text window for typing comments & results If I copy a plot and try ot paste it into >the text window, instead it pastes it into the original grpahics >graphics [sic] window. I guess text will stay text and images will stay images.... for now anyway. >Also, is there a way to copy only selected columns from the >Results Window? ( i.e. without first resetting the columns under >the Options window). You don't have to reset the counter to have the desired results be available for retrieval. You can have the macro SetOptions('Your desired column') try running the first macro, then go paste, run the second macro Macro 'Copy Area results'; begin Measure; setOptions('Area'); ShowResults; Copy; end; Macro 'Copy Mean results'; begin setOptions('Mean'); ShowResults; Copy; end; Mark Vivino NIH/DCRT mvivino@helix.nih.gov From bsimon@pennsy.med.jhu.edu Tue Jun 22 13:23:12 1993 Received: from pennsy.med.jhu.edu by nx1.soils.umn.edu (5.65c) id AA00515; Tue, 22 Jun 1993 16:07:40 -0500 Return-Path: Received: from [128.220.90.219] by pennsy.med.jhu.edu (5.65/1.34) id AA26691; Tue, 22 Jun 93 17:23:12 -0400 Date: Tue, 22 Jun 93 17:23:12 -0400 Message-Id: <9306222123.AA26691@pennsy.med.jhu.edu> To: nih-image@soils.umn.edu From: bsimon@pennsy.med.jhu.edu (Brett A. Simon) Subject: Problem with SetDensitySlice I am new at macros, and can't seem to get this simple macro to work. It is supposed to density slice a group of open images. The same macro works to threshold all the open images, replacing SetDensitySlice() with SetThreshold(). The SetDensitySlice() command works OK for a single image. What am I doing wrong? macro 'density slice all [F10]' Var i:integer Begin for i:=1 to nPics do begin SelectPic(i) SetDensitySlice(83,216) end end Thanks! _____ Brett Simon (bsimon@pennsy.med.jhu.edu) Dept. of Anesthesia and Critical Care Medicine Johns Hopkins Hospital (410) 614-1515 From Kardon-Randy@ophthalmology-po.ophth.uiowa.edu Sat Jun 22 12:42:00 1993 Received: from ns-mx.uiowa.edu by nx1.soils.umn.edu (5.65c) id AA01358; Tue, 22 Jun 1993 18:42:02 -0500 Return-Path: Received: from argos.weeg.uiowa.edu by ns-mx.uiowa.edu (5.64.jnf/930621.temp) on Tue, 22 Jun 93 18:45:17 -0500 id AA06259 with SMTP From: Received: by argos.weeg.uiowa.edu (5.64.jnf/921215) on Tue, 22 Jun 93 18:45:13 -0500 id AA13934 Message-Id: <9306222345.AA13934@argos.weeg.uiowa.edu> Received: by ccmail-gateway.uiowa.edu for cc:Mail translation to SMTP on Tue Jun 22 18:45:00 1993 To: mvivino%helix.nih.gov@relay.tc.umn.edu, Multiple recipients of list Date: 22 Jun 93 18:42 CST Subject: Software for volume render of non-aligned 3D pixel data? The best software for 3-D visualization that does an excellent job of interpolation and determination of missing points is, I believe Spyglass Transform 3.1. Spyglass also sells a volume reconstruction program, Dicer that may also be of interest to you. Sincerely, Randy Kardon MD PhD Neuro-Ophthalmology Division University of Iowa Dept Ophthalmology Iowa City Iowa 52242 From wayne@zippy.nimh.nih.gov Wed Jun 23 11:09:55 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA05945; Wed, 23 Jun 1993 11:08:43 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA07829; Wed, 23 Jun 93 11:24:18 -0400 Message-Id: <9306231524.AA07829@zippy.nimh.nih.gov> Date: Wed, 23 Jun 1993 11:09:55 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Problem with SetDensitySlice >I am new at macros, and can't seem to get this simple macro to work. It is >supposed to density slice a group of open images. The same macro works to >threshold all the open images, replacing SetDensitySlice() with >SetThreshold(). The SetDensitySlice() command works OK for a single image. > What am I doing wrong? > >macro 'density slice all [F10]' >Var > i:integer >Begin > for i:=1 to nPics do begin > SelectPic(i) > SetDensitySlice(83,216) > end >end This macro doesn't work because, unlike thresholds, Image doesn't store the density slice with the image. In other words, there is only one density slice, but there is a threshold for each image. Also, unlike thresholds, density slicing is turned off when you switch windows. You can turn it back on, however, by double clicking on the LUT tool. The differences between density slicing and thresholding should become clearer if you play with the following macros. macro 'Density Slice All' Var i:integer Begin for i:=1 to nPics do begin SelectPic(i); SetDensitySlice(i*50+50,i*50+100); end end; macro 'Threshold All' Var i:integer Begin for i:=1 to nPics do begin SelectPic(i); SetThreshold(i*50+50); end end; From glenmac@u.washington.edu Wed Jun 23 03:59:54 1993 Received: from carson.u.washington.edu by nx1.soils.umn.edu (5.65c) id AA07686; Wed, 23 Jun 1993 13:07:09 -0500 Return-Path: Received: by carson.u.washington.edu (5.65/UW-NDC Revision: 2.22 ) id AA22393; Wed, 23 Jun 93 11:10:25 -0700 Sender: glenmac@carson.u.washington.edu Date: Wed, 23 Jun 1993 10:59:54 -0700 (PDT) From: Glen Macdonald Subject: Re: Problem with SetDensitySlice To: nih-image@soils.umn.edu In-Reply-To: <9306222123.AA26691@pennsy.med.jhu.edu> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=us-ascii I ran into the same problem and overcame it after discovering that Image retains the previous density slice setting between images. at least when sequentially capturing and measuring images or manually selecting image windows. To use the macro below, I set the density slice based on an initial image, then outline a cell, or let it take the whole window, and run the macro for each roi. It turns off density slicing, measures total area, then turns density slicing back on and measures the area of pixels in the slice interval. It doesn't matter if you have density slicing on or off when running the macro. Hope this helps. -Glen macro 'Area Fraction of Density Slice [M]'; {Measures Whole cell and density sliced area, stores them into the Area and Major Axis columns and puts the decimal fraction of area in the density into the Minor Axis column.} var i,n:integer; CellArea:real; {temp variable to hold results between Measurements} AreaFrxn:real; {this variable defines the fraction of area in the density slice} begin SetOptions('Area,Major,Minor'); SetPrecision(3); SetMajorLabel('Slice Area'); SetMinorLabel('Frxn Area'); SetDensitySlice(0,0);{turn density slice off for total cell outline area} measure;{put total cell area into rArea[rCount]} CellArea:=rArea[rCount];{save this area value as the variable CellArea} SetDensitySlice(255,255); {regain density slice} SetCounter(rCount-1);{set counter back because doing two measurements for single cell} measure; {put this density sliced area into rArea[rCount]} rMajor[rCount ]:=rArea[rCount ]; {put the density sliced area in rMajor column} rMinor[rCount]:=rArea[rCount]/CellArea; rArea[rCount ]:=CellArea;{move the total area to correct location} updateResults; {write everything to the Measurement box} SetDensitySlice(0,0); {return to greyscaleto allow accurate encircling next cell} DrawBoundary;{leave a permanent outline of areas measured, but deletes pixels in the line from repeated measurements, changing subsequent values} end; On Tue, 22 Jun 1993, Brett A. Simon wrote: > I am new at macros, and can't seem to get this simple macro to work. It is > supposed to density slice a group of open images. The same macro works to > threshold all the open images, replacing SetDensitySlice() with > SetThreshold(). The SetDensitySlice() command works OK for a single image. > What am I doing wrong? > > macro 'density slice all [F10]' > Var > i:integer > Begin > for i:=1 to nPics do begin > SelectPic(i) > SetDensitySlice(83,216) > end > end > > Thanks! > _____ > Brett Simon (bsimon@pennsy.med.jhu.edu) > Dept. of Anesthesia and Critical Care Medicine > Johns Hopkins Hospital > (410) 614-1515 > > From CHARLEST@macc.wisc.edu Wed Jun 23 08:41:00 1993 Received: from vms2.macc.wisc.edu by nx1.soils.umn.edu (5.65c) id AA07888; Wed, 23 Jun 1993 13:39:53 -0500 Return-Path: Received: from VMSmail by vms2.macc.wisc.edu; Wed, 23 Jun 93 13:41 CDT Message-Id: <23062313412922@vms2.macc.wisc.edu> Date: Wed, 23 Jun 93 13:41 CDT From: Charles Thomas Subject: Halp! To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu",CHARLEST This may be a silly question, but the answer is eluding me. I have two confocal microscope images, one from the Rhodamine channel, one from the Fluorescein channel. I have made a look up table that runs from black to bright red in the intensity 0-128 and runs from black to bright green in the intensity range 129-255. I took the rhodamine image and divided all the values by 2 and took the Fluorescein image and divided all the values by 2 and added 128. So... we have a nice red image with intensity values from 0-128 and a nice green image with values from 129-255. What I'd like to do is simply add them together to make a new image displaying both simultaneously, but the paste control using AND doesn't seem to be doing the trick. Any suggestions? Thanks. Charles Thomas Integrated Microscopy Resource. PS. I'm sure we could do this with Photoshop, but I want to try to do as much image proc. around here with Image. :) From wayne@zippy.nimh.nih.gov Wed Jun 23 15:13:30 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA08466; Wed, 23 Jun 1993 15:12:19 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA08467; Wed, 23 Jun 93 15:27:54 -0400 Message-Id: <9306231927.AA08467@zippy.nimh.nih.gov> Date: Wed, 23 Jun 1993 15:13:30 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Composite color images >This may be a silly question, but the answer is eluding me. >I have two confocal microscope images, one from the Rhodamine channel, one from >the Fluorescein channel. I have made a look up table that runs from black to >bright red in the intensity 0-128 and runs from black to bright green in the >intensity range 129-255. I took the rhodamine image and divided all the values >by 2 and took the Fluorescein image and divided all the values by 2 and added >128. So... we have a nice red image with intensity values from 0-128 and a nice >green image with values from 129-255. What I'd like to do is simply add them >together to make a new image displaying both simultaneously, but the paste >control using AND doesn't seem to be doing the trick. Any suggestions? > >Thanks. > >Charles Thomas >Integrated Microscopy Resource. > >PS. I'm sure we could do this with Photoshop, but I want to try to do as much >image proc. around here with Image. :) You do this in Image essentially the same way you would in Photoshop. Create a three slice stack consiting of the Rhodamine image, the Fluorescein image and one blank(white) slice. Then invert all three slices and use the RGB to 8-bit Color command to create a composite color image. I expect to eliminate the need to invert the slices in a future version of Image --wayne From shaque@physci.ucla.edu Sun Jun 23 08:27:19 1993 Received: from uclachem.chem.ucla.edu by nx1.soils.umn.edu (5.65c) id AA10427; Wed, 23 Jun 1993 18:27:55 -0500 Return-Path: Received: from wylbur.physci.ucla.edu by uclachem.chem.ucla.edu (Sendmail 5.61/1.05) id AA27917; Wed, 23 Jun 93 16:31:11 -0700 Message-Id: <9306232331.AA27917@uclachem.chem.ucla.edu> Date: 23 Jun 1993 16:27:19 -0800 From: "Samir Haque" Subject: -RE- Image Users at UCLA-- To: "NIH-Image" RE: Image Users at UCLA We are about to convert to Image for our image processing. Before that can happen I need to demonstrate the software to my prof. so I need to know if anyone on campus who uses Image would be kind enough to show us the ropes....we really are despar#001#ate to change over as our current IP sytem is dead. Thanks in advance, Samir Haque Dept. of Physiological Science (310) 825-2248 From huff@MCCLB0.MED.NYU.EDU Wed Jun 23 16:23:45 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA11010; Wed, 23 Jun 1993 20:20:39 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-13 #2884) id <01GZQD9NFN5S0002O7@MCCLB0.MED.NYU.EDU>; Wed, 23 Jun 1993 21:23:47 EDT Date: Wed, 23 Jun 1993 21:23:45 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: Multicolor images (Re: Halp!) To: nih-image@soils.umn.edu Message-Id: <01GZQD9NMVKY0002O7@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >This may be a silly question, but the answer is eluding me. Image has only 8 bits per pixel, ever. To display two colors, you have to go to 4 bits for each color. Instead of scaling the two to 7 bits each, (0 to 127 or 128 to 255), you must scale them both to 0 to 15. Then multiply one by 16 and add the two images. Then you need a lut, which is easily made using the lut arrays (named red[i] etc I think), but remember you can't change white and black. for i := 1 to 255 do begin red[i] := i div 16; green[i] := i mod 16; blue[i] := 0; end; Not tested, getting it to work is an exercise for the reader... Please post a working solution. -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From wayne@zippy.nimh.nih.gov Thu Jun 24 12:27:10 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA15464; Thu, 24 Jun 1993 12:26:05 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA09349; Thu, 24 Jun 93 12:41:34 -0400 Message-Id: <9306241641.AA09349@zippy.nimh.nih.gov> Date: Thu, 24 Jun 1993 12:27:10 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Multicolor images (Re: Halp!) Here are two macros for displaying two images as a red and green composite. The first creates a 24-bit image(with the blue slice blank) and converts it to 8-bit composite color using the RGB to 8-bit Color command. The second scales both image to 0-16, multiplies the second by 16, combines the two by ORing them, and then generates a custom LUT to display the composite image. --wayne macro 'RGB Color Merge'; { Merges a "red" image and a "green" image to create a composite color image by creating a temporary 24-bit image and converted to 8-bits. } var i,w1,w2,h1,h2,rgb:integer; begin SaveState; if nPics<>2 then begin PutMessage('This macro operates on exactly two images.'); exit; end; SelectPic(1); GetPicSize(w1,h1); SelectPic(2); GetPicSize(w2,h2); if (w1<>w2) or (h1<>h2) then begin PutMessage('The two images must have the same width and height.'); exit; end; SetNewSize(w1,h2); SetBackground(255); MakeNewStack('RGB'); AddSlice; AddSlice; rgb:=PicNumber; SelectPic(1); SelectAll; Copy; SelectPic(rgb); SelectSlice(1); Paste; Invert; SelectPic(2); SelectAll; Copy; SelectPic(rgb); SelectSlice(2); Paste; Invert; RGBToIndexed('Custom'); SelectPic(rgb); Dispose; RestoreState; end; macro 'LUT Color Merge'; { Merges a "red" image and a "green" image to create a composite color image. Both images are scaled to 0-15, the second is multiplied by 16 and the two images are ORed and displayed using a custom red-green LUT. } var i,w1,w2,h1,h2,merged:integer; begin SaveState; if nPics<>2 then begin PutMessage('This macro operates on exactly two images.'); exit; end; SelectPic(1); GetPicSize(w1,h1); SelectPic(2); GetPicSize(w2,h2); if (w1<>w2) or (h1<>h2) then begin PutMessage('The two images must have the same width and height.'); exit; end; SetNewSize(w1,h2); MakeNewWindow('Merged'); merged:=PicNumber; SelectPic(1); SelectAll; Copy; SelectPic(merged); Paste; SelectAll; MultiplyByConstant(1/16); ChangeValues(0,0,1); ChangeValues(16,16,15); SelectPic(2); SelectAll; Duplicate('Temp'); MultiplyByConstant(1/16); ChangeValues(16,16,15); MultiplyByConstant(16); ChangeValues(0,0,1); SelectAll; Copy; SelectPic(merged); Paste; DoOr; for i:=0 to 255 do begin RedLut[i]:=(i mod 16)*16; GreenLut[i]:=(i div 16)*16; BlueLut[i]:=0; end; UpdateLut; SelectPic(nPics); Dispose; {Temp} RestoreState; end; From mvivino@helix.nih.gov Thu Jun 24 09:50:02 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA15609; Thu, 24 Jun 1993 12:46:48 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA15063; Thu, 24 Jun 93 13:50:02 -0400 Date: Thu, 24 Jun 93 13:50:02 -0400 Message-Id: <9306241750.AA15063@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: PowerPC ad in WSJ Anyone interested in the power pc chip might check out today's 10 page ad in the wall street journal. Impressive ad, must have cost a fortune. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From CHARLEST@macc.wisc.edu Thu Jun 24 09:29:00 1993 Received: from vms2.macc.wisc.edu by nx1.soils.umn.edu (5.65c) id AA16280; Thu, 24 Jun 1993 14:28:08 -0500 Return-Path: Received: from VMSmail by vms2.macc.wisc.edu; Thu, 24 Jun 93 14:29 CDT Message-Id: <23062414294160@vms2.macc.wisc.edu> Date: Thu, 24 Jun 93 14:29 CDT From: Charles Thomas Subject: Thanks and Halp again. To: nih-image@soils.umn.edu X-Vms-To: IN%"nih-image@soils.umn.edu",CHARLEST Wayne and everyone, Thanks for your prompt help on my Image question regarding the merging of two images to form a red/green composite. When I try to run the macro I get the message: Undefined identifier in line 43. <> ('Custom'); Any suggestions? From wayne@zippy.nimh.nih.gov Thu Jun 24 14:57:05 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA16532; Thu, 24 Jun 1993 14:55:57 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA09668; Thu, 24 Jun 93 15:11:35 -0400 Message-Id: <9306241911.AA09668@zippy.nimh.nih.gov> Date: Thu, 24 Jun 1993 14:57:05 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Thanks and Halp again. >Wayne and everyone, > > Thanks for your prompt help on my Image question regarding the merging >of two images to form a red/green composite. > When I try to run the macro I get the message: > Undefined identifier in line 43. <> ('Custom'); The RGBToIndexed macro command was added in V1.50. I forgot to put "Requires(1.50)" at the beginning of the macro. --wayne From kartenh@Sdsc.Edu Thu Jun 24 20:44:10 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA16848; Thu, 24 Jun 1993 15:41:05 -0500 Return-Path: Date: Thu, 24 Jun 93 20:44:10 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930624204410.2880af24@sds.sdsc.edu> Subject: RE: PowerPC ad in WSJ To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Mark, I'm impressed that you follow the WSJ! Please tell us the contents of the ad. Many thanks, Hkarten From cls7@cornell.edu Thu Jun 24 17:59:34 1993 Received: from POSTOFFICE.MAIL.CORNELL.EDU by nx1.soils.umn.edu (5.65c) id AA18453; Thu, 24 Jun 1993 20:56:21 -0500 Return-Path: Received: from [132.236.236.89] (CU-DIALUP-0315.CIT.CORNELL.EDU) by postoffice.mail.cornell.edu with SMTP id AA03824 (5.65c8/IDA-1.4.4 for nih-image@soils.umn.edu); Thu, 24 Jun 1993 21:59:34 -0400 Date: Thu, 24 Jun 1993 21:59:34 -0400 Message-Id: <199306250159.AA03824@postoffice.mail.cornell.edu> To: nih-image@soils.umn.edu From: cls7@cornell.edu (Carl Schofield) X-Sender: cls7@postoffice.mail.cornell.edu Subject: Image 1.50b75/QuickImage 24 The QuickImage 24 plug-in module for Adobe Photoshop also works just fine with Image v.1.50b75. Possible to utilize frame averaging or summation to improve image quality with this frame grabber? From jolyar@cumc.cornell.edu Thu Jun 24 21:04:25 1993 Received: from cumc.cornell.edu (PATHMAC.MED.CORNELL.EDU) by nx1.soils.umn.edu (5.65c) id AA19358; Fri, 25 Jun 1993 00:08:14 -0500 Return-Path: Received: by cumc.cornell.edu (5.65c/ECH1.13) id AA26766; Fri, 25 Jun 1993 01:11:37 -0400 Posted-Date: Fri, 25 Jun 1993 01:04:25 -0400 (EDT) Date: Fri, 25 Jun 1993 01:04:25 -0400 (EDT) From: John Olyarchuk Sender: John Olyarchuk Reply-To: John Olyarchuk Subject: RE: PowerPC ad in WSJ To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <930624204410.2880af24@sds.sdsc.edu> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII On Thu, 24 Jun 1993, Harvey Karten wrote: > Mark, > I'm impressed that you follow the WSJ! Please tell us the contents > of the ad. > Many thanks, Hkarten > While we are discussing press events, did anyone see the review of Image in this months Macworld? Actually, the article refers to ProVision II which is a modified version of Image that is packaged with the CD collection Amazing Universe 2.1. Credit is given to Wayne, although Image is refered to as Images(sic). Included among the commentary in the review is..."ProVision is in most respects a more powerful image-modification program than Adobe Photoshop" ..."As a piece of commercial software with proper documentation, ProVision would sell for about $695... Incidently, the CD lists for $79.95. BTW, has anyone tried using Kai's Power Tools plug-ins with Image? Do they work? Are they useful? Are they fun? -John G. Olyarchuk Cornell University Medical College From r.m.ward@bham.ac.uk Fri Jun 25 11:41:32 1993 Received: from bham.ac.uk by nx1.soils.umn.edu (5.65c) id AA20903; Fri, 25 Jun 1993 05:38:23 -0500 Return-Path: Received: from diana.met.bham.ac.uk by bham.ac.uk with SMTP (PP) id <17719-0@bham.ac.uk>; Fri, 25 Jun 1993 11:29:42 +0100 Message-Id: <10680.9306251041@diana.met.bham.ac.uk> Received: from tucker.met.bham.ac.uk by diana.met.bham.ac.uk; Fri, 25 Jun 1993 11:41:50 +0100 Date: Fri, 25 Jun 1993 11:41:32 +0000 To: nih-image@soils.umn.edu From: r.m.ward@bham.ac.uk (Mark Ward) X-Sender: r.m.ward@diana.met.bham.ac.uk Subject: kingfisher video board plug-in Dear all, Image1.50b75 also works fine with the Kingfisher frame-grabber from Graphics Unlimited, Cambridge, UK, via the photoshop plug-in supplied. p.s. I'm new to this group, so if someone's already said this, my apologies..! From huff@MCCLB0.MED.NYU.EDU Fri Jun 25 01:55:04 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA21086; Fri, 25 Jun 1993 05:52:16 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-13 #2884) id <01GZSBIB2TPC0006T1@MCCLB0.MED.NYU.EDU>; Fri, 25 Jun 1993 06:55:04 EDT Date: Fri, 25 Jun 1993 06:55:04 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: How about officially renaming Image to NIHImage? To: nih-image@soils.umn.edu Message-Id: <01GZSBIBB4OY0006T1@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT People who are several 9600 baud or slower links away from zippy try to use archie servers to search for a nearby FTP site to obtain software. Searching for Image this way is literally impossible, because there are so many irrelevant instances of the string "image" in the archie database. Officially changing the file name to NIHImage1.50.sit.hqx would make it possible to use archie to find it. Also there is the conflict with NCSAImage... -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From ptr@GRECO2.POLYTECHNIQUE.FR Fri Jun 25 15:09:47 1993 Received: from chenas.inria.fr by nx1.soils.umn.edu (5.65c) id AA21214; Fri, 25 Jun 1993 06:08:46 -0500 Return-Path: Received: from polytechnique.polytechnique.fr by chenas.inria.fr (5.65c8d/92.02.29) via Fnet-EUnet id AA25237; Fri, 25 Jun 1993 13:11:32 +0200 (MET) Received: by polytechnique.polytechnique.fr (5.65c/SMI-4.1.3) id AA25480; Fri, 25 Jun 1993 13:18:07 +0200 Received: from [129.104.27.245] by greco2.noname (4.1/SMI-4.1) id AA05064; Fri, 25 Jun 93 13:09:47 +0200 Date: Fri, 25 Jun 93 13:09:47 +0200 Message-Id: <9306251109.AA05064@greco2.noname> To: From: ptr@GRECO2.POLYTECHNIQUE.FR (Peter Goedtkindt) Subject: Re: kingfisher video board plug-in >Dear all, > Image1.50b75 also works fine with the Kingfisher frame-grabber from >Graphics Unlimited, Cambridge, UK, via the photoshop plug-in supplied. >p.s. I'm new to this group, so if someone's already said this, my apologies..! What a coincidence, I was just planning to write the same: The sales women just left the laboratory, taking the demo card with her back. A remark: *The external trigger is not availiable in the plug-in, but it exists in the kingfisher application. I still have some unsolved questions: * how accurate is the external trigger. we need to capture the frame (only one) in which the external trigger signal falls to ground (and not the next one). The trigger signal needs to be 250 ms long, as stated in the manual, and this makes me think there in a possible problem. *We want to capture three signals, coming from synchronised b/w cameras, using the same trigger signal. I've taken some test images and the card takes the three images in parallel (this is perfect). But I also noticed that the color separation was far from perfect: suppose the camera connected to the red input is in the dark, and the green has an intense image, then the red image will have a ghost copy from the green input. Since I only could test the card for a limited time, it would be interesting if someone else could complete this information. TIA Peter Goedtkindt Universite Paris-Sud Laboratoire de Spectroscopie Atomique et Ionique bat350 Centre D'Orsay F-91405 Orsay Cedex. France Fax.:++33.1.69.41.94.60 - Tel 69.41.77.10 ------ And ------ Ecole Polytechnique, Laboratoire d'Utilisation de Lasers Intenses tel. 69.33.48.95 ----------------------------------------------------------------- From ptr@GRECO2.POLYTECHNIQUE.FR Fri Jun 25 15:14:35 1993 Received: from chenas.inria.fr by nx1.soils.umn.edu (5.65c) id AA21248; Fri, 25 Jun 1993 06:13:14 -0500 Return-Path: Received: from polytechnique.polytechnique.fr by chenas.inria.fr (5.65c8d/92.02.29) via Fnet-EUnet id AA25368; Fri, 25 Jun 1993 13:16:20 +0200 (MET) Received: by polytechnique.polytechnique.fr (5.65c/SMI-4.1.3) id AA25524; Fri, 25 Jun 1993 13:22:55 +0200 Received: from [129.104.27.245] by greco2.noname (4.1/SMI-4.1) id AA05068; Fri, 25 Jun 93 13:14:36 +0200 Date: Fri, 25 Jun 93 13:14:35 +0200 Message-Id: <9306251114.AA05068@greco2.noname> To: From: ptr@GRECO2.POLYTECHNIQUE.FR (Peter Goedtkindt) Subject: Re:renaming Image to NIHImage? >Officially changing the file name to NIHImage1.50.sit.hqx would make it >possible to use archie to find it. Also there is the conflict with >NCSAImage... >-- >Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 I agree completely, WayneImage wouldn't be bad either.... Peter Goedtkindt Universite Paris-Sud Laboratoire de Spectroscopie Atomique et Ionique bat350 Centre D'Orsay F-91405 Orsay Cedex. France Fax.:++33.1.69.41.94.60 - Tel 69.41.77.10 ------ And ------ Ecole Polytechnique, Laboratoire d'Utilisation de Lasers Intenses tel. 69.33.48.95 ----------------------------------------------------------------- From mvivino@helix.nih.gov Fri Jun 25 04:20:28 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA21636; Fri, 25 Jun 1993 07:17:14 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA03069; Fri, 25 Jun 93 08:20:28 -0400 Date: Fri, 25 Jun 93 08:20:28 -0400 Message-Id: <9306251220.AA03069@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: RE: PowerPC ad in WSJ > I'm impressed that you follow the WSJ! Please tell us the contents >of the ad. Lots of blank space. Pictures of the IC. Lots of big statements of Apple and IBM both being behind the chip. Mentions of the distant Taligent and other OS'es which can use the PowerPC. And of course, the much too much expected comparisons to the Pentium chip. I think I even saw ads for this ad somewhere last week. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From morgan@rock.concert.net Fri Jun 25 04:36:49 1993 Received: from rock.concert.net by nx1.soils.umn.edu (5.65c) id AA21747; Fri, 25 Jun 1993 07:33:35 -0500 Return-Path: Received: by rock.concert.net (5.59/tas-rock/8-12-92) id AA27425; Fri, 25 Jun 93 08:36:49 -0400 From: Morgan J Ryan -- Neil Patterson Publishers X-Disclaimer-1: rock is a CONCERT-CONNECT public access host. X-Disclaimer-2: Opinions expressed are not necessarily X-Disclaimer-3: those of MCNC or the CONCERT Network. Message-Id: <9306251236.AA27425@rock.concert.net> Subject: Re: How about officially renaming Image to NIHImage? To: nih-image@soils.umn.edu Date: Fri, 25 Jun 93 8:36:49 EDT In-Reply-To: <01GZSBIBB4OY0006T1@MCCLB0.MED.NYU.EDU>; from "Edward J. Huff" at Jun 25, 93 5:59 am X-Mailer: ELM [version 2.3 PL11] If you tell archie to look for Image150 or 1.50 you won't get all the false leads. I'm not sure if archie reads the dot. Morgan Ryan From mvivino@helix.nih.gov Fri Jun 25 05:04:47 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA22067; Fri, 25 Jun 1993 08:10:35 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA08393; Fri, 25 Jun 93 09:04:47 -0400 Date: Fri, 25 Jun 93 09:04:47 -0400 Message-Id: <9306251304.AA08393@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Extra possibility for the text window? Here is a suggestion for the text window. Ah, this is not asking for a complete editor like microsoft word, but actually something useful to a macro writer. I'd like to have the line number visible to the left of the line, or I'd like a goto line number statement to be entered somehow. For example: you enter gotoline(300) and the code starts at the window text buffer[0] counting the CR characters. When it reaches the 300th CR the text is scrolled to this point and the cursor is placed down at this point. Nice idea I suppose, but it would be better if I were offering the code that did this. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From wayne@zippy.nimh.nih.gov Fri Jun 25 08:49:38 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22260; Fri, 25 Jun 1993 08:48:29 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA10556; Fri, 25 Jun 93 09:04:12 -0400 Message-Id: <9306251304.AA10556@zippy.nimh.nih.gov> Date: Fri, 25 Jun 1993 08:49:38 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: How about officially renaming Image to NIHImage? >People who are several 9600 baud or slower links away from zippy try to use >archie servers to search for a nearby FTP site to obtain software. >Searching for Image this way is literally impossible, because there are so >many irrelevant instances of the string "image" in the archie database. >Officially changing the file name to NIHImage1.50.sit.hqx would make it >possible to use archie to find it. Also there is the conflict with >NCSAImage... Sounds like a good idea to me. I have changed all instances of the string "image" to "nih-image" on Zippy. --wayne From huff@MCCLB0.MED.NYU.EDU Fri Jun 25 05:06:05 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA22392; Fri, 25 Jun 1993 09:03:01 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-13 #2884) id <01GZSI74RKMO00018B@MCCLB0.MED.NYU.EDU>; Fri, 25 Jun 1993 10:06:07 EDT Date: Fri, 25 Jun 1993 10:06:05 -0500 From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) Subject: RE: How about officially renaming Image to NIHImage? To: nih-image@soils.umn.edu Message-Id: <01GZSI74ZUO200018B@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu Content-Transfer-Encoding: 7BIT >Sounds like a good idea to me. I have changed all instances of the string >"image" to "nih-image" on Zippy. Thanks, but I think you better put some symbolic links in for the old directory names. I just looked with Fetch, and got "/pub/image" does not exist. Having just become a Unix hacker (khoros is almost running on our Sparc10 with Solaris 2.2), thats "ln -s /pub/nih-image /pub" to make /pub/image continue to work. Or is it the other way? You can't tell from the turgid prose of "man ln"... Just have to try it... -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From wayne@zippy.nimh.nih.gov Fri Jun 25 09:09:01 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22517; Fri, 25 Jun 1993 09:07:52 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA10637; Fri, 25 Jun 93 09:23:35 -0400 Message-Id: <9306251323.AA10637@zippy.nimh.nih.gov> Date: Fri, 25 Jun 1993 09:09:01 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Extra possibility for the text window? >Here is a suggestion for the text window. Ah, this is not asking for a >complete editor like microsoft word, but actually something useful to a >macro writer. I'd like to have the line number visible to the left of the >line, or I'd like a goto line number statement to be entered somehow. > >For example: you enter gotoline(300) and the code starts at the window text >buffer[0] counting the CR characters. When it reaches the 300th CR the text >is scrolled to this point and the cursor is placed down at this point. > >Nice idea I suppose, but it would be better if I were offering the code >that did this. I will consider some sort of goto option when I get around to working on a text Find command. It would also be nice to have an Undo for text changes and to have text windows listed in the Windows menu. --wayne From steven@cell.BIH.Harvard.Edu Fri Jun 25 06:26:27 1993 Received: from cell.bih.harvard.edu by nx1.soils.umn.edu (5.65c) id AA22682; Fri, 25 Jun 1993 09:23:27 -0500 Return-Path: Received: by cell.bih.harvard.edu (4.1/SMI-DDN) id AA02177; Fri, 25 Jun 93 10:26:27 EDT Date: Fri, 25 Jun 93 10:26:27 EDT From: steven@cell.BIH.Harvard.Edu (Steven Blechner) Message-Id: <9306251426.AA02177@cell.bih.harvard.edu> To: nih-image@soils.umn.edu Subject: Serial port problems... I have a problem with controlling a Ludl Electronics controller (for shutter control and filter wheel positions, etc.). 1. If I run Image, open the serial port with a macro, and try to send anything accross the serial line, I crash the system and must reboot... 2. If I first open White Knight (communications program) and then open image, I can do whatever I want over the serial line... I checked the settings for White Knight versus the settings for my macro serial call, and they are identical... Has anyone else had problems controlling things via a serial port from within Image ? -steve From wayne@zippy.nimh.nih.gov Fri Jun 25 09:32:41 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22742; Fri, 25 Jun 1993 09:31:32 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA10704; Fri, 25 Jun 93 09:47:15 -0400 Message-Id: <9306251347.AA10704@zippy.nimh.nih.gov> Date: Fri, 25 Jun 1993 09:32:41 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: RE: How about officially renaming Image to NIHImage? >Thanks, but I think you better put some symbolic links in for the old >directory names. I just looked with Fetch, and got "/pub/image" does not >exist. > >Having just become a Unix hacker (khoros is almost running on our Sparc10 >with Solaris 2.2), thats "ln -s /pub/nih-image /pub" to make /pub/image >continue to work. Or is it the other way? You can't tell from the >turgid prose of "man ln"... Just have to try it... I used the command "ln -s nih-image image" to create an alias named image that points to the directory nih-image. Having to use cryptic UNIX commands such as this one makes you appreciate the Macintosh. --wayne From jladwig@soils.umn.edu Fri Jun 25 05:18:45 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA23131; Fri, 25 Jun 1993 10:16:46 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA15947; Fri, 25 Jun 93 10:18:45 CDT Date: Fri, 25 Jun 93 10:18:45 CDT From: "John Ladwig" Message-Id: <9306251518.AA15947@saturn.soils.umn.edu> To: Subject: RE: How about officially renaming Image to NIHImage? In-Reply-To: Wayne Rasband's message <9306251347.AA10704@zippy.nimh.nih.gov> of 25 June 1993 References: <9306251347.AA10704@zippy.nimh.nih.gov> Wayne Rasband writes on 25 June 1993 at 09:31:41 -0500 > >Having just become a Unix hacker (khoros is almost running on our Sparc10 > >with Solaris 2.2), thats "ln -s /pub/nih-image /pub" to make /pub/image > >continue to work. Or is it the other way? You can't tell from the > >turgid prose of "man ln"... Just have to try it... > > I used the command "ln -s nih-image image" to create an alias named image > that points to the directory nih-image. Having to use cryptic UNIX commands > such as this one makes you appreciate the Macintosh. *Ahem* (Clears throat politely, takes a deep breath, and decides *not* to don the asbestos overcoat :-) Might I suggest that bashing Unix in a mailing list which is *run* on Unix might be akin to biting the hand... :-) I *will not* start an OS flamefest, I *will not* start an OS flamefest, I *will not* start an OS flamefest, I *will not* start an OS flamefest, I *will not* start an OS flamefest... -jml NIH-Image mailing list administrator From kartenh@Sdsc.Edu Fri Jun 25 17:00:21 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA23891; Fri, 25 Jun 1993 11:57:31 -0500 Return-Path: Date: Fri, 25 Jun 93 17:00:21 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930625170021.28812e1c@sds.sdsc.edu> Subject: Re: How about officially renaming Image to NIHImage? To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH I preferred the name Wayne_Image or Rasband_Image. It reflects our communal gratitude to you. many thanks, as always Harvey From mvivino@helix.nih.gov Fri Jun 25 09:11:08 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA24024; Fri, 25 Jun 1993 12:07:55 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA09325; Fri, 25 Jun 93 13:11:08 -0400 Date: Fri, 25 Jun 93 13:11:08 -0400 Message-Id: <9306251711.AA09325@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: How about officially renaming Image to NIHImage? I'm surprised nobody has suggested "MacImage". NIHImage as a name brings lots of thanks from the public to NIH, so helps us justify tax dollars..... Mark Vivino NIH/DCRT mvivino@helix.nih.gov From GLIKSMAN@uncvx1.oit.unc.edu Fri Jun 25 08:22:26 1993 Received: from uncvx1.oit.unc.edu by nx1.soils.umn.edu (5.65c) id AA24083; Fri, 25 Jun 1993 12:16:41 -0500 Return-Path: Received: from uncvx1.oit.unc.edu by uncvx1.oit.unc.edu (PMDF V4.2-11 #3863) id <01GZSP0CVKBK001GWM@uncvx1.oit.unc.edu>; Fri, 25 Jun 1993 13:22:26 EST Date: Fri, 25 Jun 1993 13:22:26 -0500 (EST) From: GLIKSMAN@uncvx1.oit.unc.edu Subject: Re: How about officially renaming Image to NIHImage? To: nih-image@soils.umn.edu Message-Id: <01GZSP0CWWJM001GWM@uncvx1.oit.unc.edu> X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT How about ending this subject and talking about the program, its applications or other useful subjects. -Neal Gliksman From chien@jeeves.UCSD.EDU Fri Jun 25 03:38:38 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA24314; Fri, 25 Jun 1993 12:35:27 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA26700 sendmail 5.67/UCSD-2.2-sun via SMTP Fri, 25 Jun 93 10:38:40 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA10681 to nih-image@soils.umn.edu; Fri, 25 Jun 93 10:38:38 PDT Date: Fri, 25 Jun 93 10:38:38 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306251738.AA10681@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: Neotech Image Grabber Photoshop plug-in Since we're on this subject, I tried the Photoshop plug-in for the Neotech Image Grabber board that we have with Image v1.50b52, it seems to work fine. This plug-in provides support for live-preview, single-frame grabs, averaged grabs (using the Mac for averaging, there's only one frame of on-board memory), and automatic exposure control. Also live preview. Unfortunately, it doesn't support external triggers or manual gain/black level control, two of the main reasons that I bought that board. I've had good results with it for almost two years, using Quickeys to switch back and forth between Image and the Image Grabber's own application, but I'm about to buy the Scion LG-3. For those wondering about choices of boards, I have three main reasons: 1) the Scion can be controlled directly from NIH-Image, 2) the Scion's digital and analog outputs will be perfect for controlling a Uniblitz shutter (I've been using a little Tandy 102 portable up until now), and 3) the Scion will allow me to take several frames at video rates and average them. We make time-lapse movies of fluorescently- labelled growing axons, and need to minimize exposure times. Currently I use 80 ms flashes and just grab one video frame during that flash; I expect that lengthening the flash to perhaps 120 ms and grabbing four frames during that time will significantly improve the images. Chi-Bin Chien PS--Having just read the name-change messages within the last ten minutes, I feel justified in using up a few baud-seconds of bandwidth to say that it's a good idea, especially to avoid confusion with NCSA Image. From herro001@staff.tc.umn.edu Fri Jun 25 08:12:59 1993 Received: from staff.tc.umn.edu by nx1.soils.umn.edu (5.65c) id AA24516; Fri, 25 Jun 1993 13:09:44 -0500 Return-Path: Message-Id: <199306251809.AA24516@nx1.soils.umn.edu> Received: from rash.med.umn.edu by staff.tc.umn.edu id SMTP-0012c2b4019017006; Fri, 25 Jun 93 13:12:43 -0500 From: "Mike Herron" Reply-To: "Mike Herron" To: nih-image@soils.umn.edu Subject: Re: Neotech Image Grabber Photoshop plug-in Date: Fri, 25 Jun 93 13:12:59 -0500 In message <9306251738.AA10681@jeeves.UCSD.EDU> writes: > 2) the Scion's digital and analog outputs will be perfect for > controlling a Uniblitz shutter (I've been using a little Tandy 102 portable > up until now) Could you tell me the distributor and or phone for the Uniblitz shutter? UNIBLITZ <--great name :^) From ac24@cornell.edu Fri Jun 25 11:03:20 1993 Received: from POSTOFFICE.MAIL.CORNELL.EDU by nx1.soils.umn.edu (5.65c) id AA24823; Fri, 25 Jun 1993 13:52:24 -0500 Return-Path: Received: from [132.236.7.8] by postoffice.mail.cornell.edu with SMTP id AA13391 (5.65c8/IDA-1.4.4 for nih-image@soils.umn.edu); Fri, 25 Jun 1993 14:55:35 -0400 Message-Id: <199306251855.AA13391@postoffice.mail.cornell.edu> Date: Fri, 25 Jun 1993 15:03:20 -0400 To: From: ac24@cornell.edu (Ary Correa) X-Sender: ac24@postoffice.mail.cornell.edu Subject: Re: Neotech Image Grabber Photoshop plug-in >In message <9306251738.AA10681@jeeves.UCSD.EDU> writes: >> 2) the Scion's digital and analog outputs will be perfect for >> controlling a Uniblitz shutter (I've been using a little Tandy 102 portable >> up until now) > >Could you tell me the distributor and or phone for the Uniblitz shutter? > >UNIBLITZ <--great name :^) You may call Vincent Associates 1255 University Avenue - Rochester, N.Y. 14607 (716)4732232 (800)8286972 From set@eru.mt.luth.se Mon Jun 28 18:45:20 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA13354; Mon, 28 Jun 1993 10:42:11 -0500 Return-Path: Received: from [130.240.1.167] (osse210.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA04316; Mon, 28 Jun 1993 17:45:18 +0200 Message-Id: <199306281545.AA04316@eru.mt.luth.se> Date: Mon, 28 Jun 1993 17:45:20 +0100 To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: Re: Radius Rocket 33 X-Content-Type: Text/Quoted-Readable >We looked closely at the Rocket, and ended up not being able to keep >it working. Radius makes excellent products and has some of the best >technical support practices and people in the industry, but the Rocket >is not entirely stable and probably never will be. Much of the nominal >speed advantage is lost in practice to bus arbitration, even though the >Rocket uses block mode transfers; mysterious crashes kept crashing the >IIcx we tried to accelerate, which the Rocket's principal engineer >attributed to "errors in Apple's 32-bit compatibility code." I do have a MacII and are using a DOVE low cost accelerator chip with no problem since 1 year. It's a 33MHz 68030 card to replace the std 68020 chip. The adress are Dove Computer Corporation 1200 North 23rd street Wilmington NC ,28405 (919) 763 7918 Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From set@eru.mt.luth.se Mon Jun 28 18:51:00 1993 Received: from eru.mt.luth.se by nx1.soils.umn.edu (5.65c) id AA13409; Mon, 28 Jun 1993 10:47:52 -0500 Return-Path: Received: from [130.240.1.167] (osse210.mt.luth.se) by eru.mt.luth.se with SMTP (5.65+bind 1.7+ida 1.4.2/IDA-1.2.8-NS) id AA04529; Mon, 28 Jun 1993 17:50:59 +0200 Message-Id: <199306281550.AA04529@eru.mt.luth.se> Date: Mon, 28 Jun 1993 17:51:00 +0100 To: From: set@mt.luth.se (Sven-Erik Tiberg) Subject: Re: call for help X-Content-Type: Text/Quoted-Readable >This is an inquiry and call for help: > >We have a Scion LG-3 frame grabber board with 16 MB of video RAM. > >We need to capture several (approx. 30) frames at video rate and >subsequently transfer the frames to a stack (without averaging). Start and >stop capturing commands should be two different macro commands. The stop >command should include the transfer of all the frames in the LG-3 video RAM >to a stack in the computer RAM. > >Also, during the video rate capturing the settings of the two Digital to >Analog converters on the LG-3 (Scion[1] and Scion [2]) need to be changed >independently, without affecting the video rate capture. > >Has anyone implemented such macro functions (or similar ones)? >Who else is interested in such macro functions? >Wayne, would you consider implementing these functions? >------------- >Guang Mei gmei@hoh.mbl.edu Tel:(508)548-3705 ext 374 >Program of Architectural Dynamics in Living Cells >Marine Biological Laboratory, Woods Hole, MA 02543-1031 We do have the same problem, and do urgently need it before oct. In case nobody else has done it or will do so in the near future we will do it. Sven-Erik Tiberg Div. of energy / mashineenginnering Lulea University of Technology Lulea Sweden email set@eru.mt.luth.se Fax. S-(0)920-91047 Tel. S-(0)920-91218, S-(0)10-2189638 From grcunha@itsa.ucsf.EDU Mon Jun 28 04:07:37 1993 Received: from itsa.ucsf.EDU by nx1.soils.umn.edu (5.65c) id AA14278; Mon, 28 Jun 1993 13:10:45 -0500 Return-Path: Received: by itsa.ucsf.EDU (AIX 3.2/UCB 5.64/GSC4.22) id AA52201; Mon, 28 Jun 1993 11:07:37 -0700 Date: Mon, 28 Jun 1993 11:07:37 -0700 From: grcunha@itsa.ucsf.EDU (Gerald Cunha) Message-Id: <9306281807.AA52201@itsa.ucsf.EDU> To: nih-image@soils.umn.edu Subject: Output Advice Needed pow Can someone out there give us some advice on printers/output?# # We are looking to upgrade our digital imaging/image analysis system. At present # we get fairly decent gray scale images on our monitor from our Dage MTI # CCD72 camera using Image and Dapple's PrismView software. The printed # output from our LaserWriter+ is, or course, of much poorer quality from what we # get on the screen. Is there an affordable output alternative for the working # laboratory? The new 600 dpi printers are better, but still leave something to be # desired. Good quality output would be one more step away from having to deal # with the mess and wait of conventional photography.# # Joel Brody# Dept of Anatomy (0452)# University of California# SF, CA 94143# email grcunha@itsa.ucsf.edu# From Carl_Gustafson@cbis.ece.drexel.edu Mon Jun 28 15:28:15 1993 Received: from cbis.ece.drexel.edu by nx1.soils.umn.edu (5.65c) id AA15020; Mon, 28 Jun 1993 15:28:15 -0500 Return-Path: Received: from homer.ece.drexel.edu by cbis.ECE.Drexel.EDU (5.67/1.34) id AA14393; Mon, 28 Jun 93 15:22:08 EDT Message-Id: <9306281922.AA14393@cbis.ECE.Drexel.EDU> Date: 28 Jun 1993 14:26:13 U From: "Carl Gustafson" Subject: Re: Output Advice Needed To: nih-image@soils.umn.edu Reply to: RE>Output Advice Needed >Can someone out there give us some advice on printers/output? >We are looking to upgrade our digital imaging/image analysis system. At >present we get fairly decent gray scale images on our monitor from our >Dage MTI CCD72 camera using Image and Dapple's PrismView software. >The printed output from our LaserWriter+ is, or course, of much poorer >quality from what we get on the screen. Is there an affordable output >alternative for the working laboratory? The new 600 dpi printers are >better, but still leave something to be desired. Good quality output >would be one more step away from having to deal with the mess and wait >of conventional photography.# The solution to your problem would be a dye-sublimation printer. These printers are capable of near-photographic quality output, in both color and gray scale. The catch is that most of them are $10K+, and they are expensive to operate (about $2 per page). I don't have any information on specific printers handy, but perusing the recent MacWEEK or MacUser issues should give you the info you need. Carl Gustafson Imaging and Computer Vision Center Drexel University From grcunha@itsa.ucsf.EDU Mon Jun 28 15:19:35 1993 Received: from itsa.ucsf.EDU by nx1.soils.umn.edu (5.65c) id AA17012; Tue, 29 Jun 1993 00:22:44 -0500 Return-Path: Received: by itsa.ucsf.EDU (AIX 3.2/UCB 5.64/GSC4.22) id AA22079; Mon, 28 Jun 1993 22:19:35 -0700 Date: Mon, 28 Jun 1993 22:19:35 -0700 From: grcunha@itsa.ucsf.EDU (Gerald Cunha) Message-Id: <9306290519.AA22079@itsa.ucsf.EDU> To: nih-image@soils.umn.edu Subject: More Output Advice Thanks for the info on Dye-sublimation and 600dpi printers. Since I asked for help I received some propaganda on another possible alternative which I wonder if anyone has any experience with. Both Xante and LaserMaster have a series of laser printers that they claim is upgradable to 1200x1200 dpi. The printing sample sent by Xante is 960x960 and it really knocks your socks off. The LaserMaster person I spoke to over the phone claimed that theirs could print 256 levels of gray. I certainly want to take a test drive on their machines with some of our 'real world' images, but I would like to know if anyone out there hastried one. I'll keep you all informed of what I see. Joel Brody Dept of Anatomy U of Cal SF, CA 94143-0452 From kartenh@Sdsc.Edu Tue Jun 29 16:53:49 1993 Received: from Sdsc.Edu (sds.sdsc.edu) by nx1.soils.umn.edu (5.65c) id AA19467; Tue, 29 Jun 1993 11:50:46 -0500 Return-Path: Date: Tue, 29 Jun 93 16:53:49 GMT From: kartenh@Sdsc.Edu (Harvey Karten) Message-Id: <930629165349.28a02b72@sds.sdsc.edu> Subject: RE: More Output Advice To: nih-image@soils.umn.edu X-St-Vmsmail-To: ST%"nih-image@soils.umn.edu" X-St-Vmsmail-Cc: KARTENH Re: Xante and LaserMaster - Attn: Joel Brody What's the price on the Xante and LaserMaster. Harvey Karten Harvey J. Karten, M.D. Dept. of Neurosciences Univ.California San Diego La Jolla, CA 92093-0608 EMail: Kartenh@sdsc.edu Phone: (619)-534-4938 FAX: (619)-534-6602 From herro001@staff.tc.umn.edu Tue Jun 29 08:29:45 1993 Received: from staff.tc.umn.edu by nx1.soils.umn.edu (5.65c) id AA20025; Tue, 29 Jun 1993 13:26:37 -0500 Return-Path: Message-Id: <199306291826.AA20025@nx1.soils.umn.edu> Received: from rash.med.umn.edu by staff.tc.umn.edu id SMTP-0012c30894a022245; Tue, 29 Jun 93 13:26:21 -0500 From: "Mike Herron" Reply-To: "Mike Herron" To: nih-image@soils.umn.edu Subject: Re: More Output Advice Date: Tue, 29 Jun 93 13:29:45 -0500 In message <9306290519.AA22079@itsa.ucsf.EDU> writes: > Thanks for the info on Dye-sublimation and 600dpi printers. Since I asked > for help I received some propaganda on another possible alternative which > I wonder if anyone has any experience with. Both Xante and LaserMaster have > a series of laser printers that they claim is upgradable to 1200x1200 dpi. > The printing sample sent by Xante is 960x960 and it really knocks your socks > off. The LaserMaster person I spoke to over the phone claimed that theirs > could print 256 levels of gray. > I certainly want to take a test drive on their machines with some of our > 'real > world' images, but I would like to know if anyone out there hastried one. > I'll > keep you all informed of what I see. I did some consulting work with a small print shop that had a Lasermaster 1200dpi. Lasermaster seemed like a good company and they were quite willing to help....but I did seem to find myself hassling with their drivers et al quite a bit. I don't imagine that any other brand is any better though. My only advice is that you test their machine with _YOUR_ computer setup. Also there are some much cheaper systems with smaller images (circa 3x5 inches) reviewed on a MacWeek about a month ago. These units were continuous tone for aroun $3K I believe. From grcunha@itsa.ucsf.EDU Tue Jun 29 10:00:54 1993 Received: from itsa.ucsf.EDU by nx1.soils.umn.edu (5.65c) id AA01929; Tue, 29 Jun 1993 19:03:06 -0500 Return-Path: Received: by itsa.ucsf.EDU (AIX 3.2/UCB 5.64/GSC4.22) id AA16249; Tue, 29 Jun 1993 17:00:54 -0700 Date: Tue, 29 Jun 1993 17:00:54 -0700 From: grcunha@itsa.ucsf.EDU (Gerald Cunha) Message-Id: <9306300000.AA16249@itsa.ucsf.EDU> To: nih-image@soils.umn.edu Subject: Output Device Q&A To: Harvey Karten I don't have the price list for LaserMaster yet. Xante's is as follows: 960x960 $5495 1200x1200 $6695 (48M RAM) 1200x1200 $7895 (64M RAM) Their number is 1-800-926-8839. Joel Brody grcunha@itsa.ucsf.edu From gshiva@eng.umd.edu Tue Jun 29 18:06:46 1993 Received: from filter.eng.umd.edu by nx1.soils.umn.edu (5.65c) id AA02433; Tue, 29 Jun 1993 21:02:50 -0500 Return-Path: Received: by filter.eng.umd.edu (5.65c/IDA-1.4.4) id AA03275; Tue, 29 Jun 1993 22:06:46 -0400 Rcpt to:mailed to nih-image@soils.umn.edu Date: Tue, 29 Jun 1993 22:06:46 -0400 From: Shivakumar Gopalakrishnan Message-Id: <199306300206.AA03275@filter.eng.umd.edu> To: nih-image@soils.umn.edu Subject: Want more from the PLOT PROFILE When I save the plot profile as a text file it saves the pixel value. Can that be modified such that I can get the X Y values of the plot profile ? Please help. Thanking you G. Shivakumar From wayne@zippy.nimh.nih.gov Wed Jun 30 09:09:30 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA04854; Wed, 30 Jun 1993 09:07:32 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA16567; Wed, 30 Jun 93 09:24:32 -0400 Message-Id: <9306301324.AA16567@zippy.nimh.nih.gov> Date: Wed, 30 Jun 1993 09:09:30 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Want more from the PLOT PROFILE >When I save the plot profile as a text file it saves the >pixel value. Can that be modified such that I can get the >X Y values of the plot profile ? >Please help. There are a couple of things you could do. One would be to compute the XY coordinates based on the starting coordinates and on the fact that the samples are spaced one pixel width apart(V1.49 or later). The other would be to write a profile ploting macro that saved the XY coordenates in two of the results arrays. --wayne From chien@jeeves.UCSD.EDU Wed Jun 30 04:43:13 1993 Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA06134; Wed, 30 Jun 1993 13:39:11 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA16903 sendmail 5.67/UCSD-2.2-sun via SMTP Wed, 30 Jun 93 11:43:15 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA22972 to nih-image@soils.umn.edu; Wed, 30 Jun 93 11:43:13 PDT Date: Wed, 30 Jun 93 11:43:13 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9306301843.AA22972@jeeves.UCSD.EDU> To: nih-image@soils.umn.edu Subject: hardcopy On the subject of grayscale hardcopy, I had beautiful results using a Linotronic printer at the UCSD campus graphics facility. This is a 2400 dpi Postscript device, I just brought over a floppy with a Postscript file made by Canvas, it cost $5-$10 per copy and looks like a photograph (except with dust- or smudge-marks from the negative!). It was a little slow, probably because of the mixture of bitmap and text objects, next time I'd do it all in Photoshop for cleaner output. Many commercial printshops have Linotronics, as well. For color output, we're about to buy a confocal microscope and also need to solve this problem... Chi-Bin Chien chien@jeeves From crc@leicester.ac.uk Thu Jul 1 05:41:32 1993 Received: from sun2.nsfnet-relay.ac.uk by nx1.soils.umn.edu (5.65c) id AA10154; Thu, 1 Jul 1993 05:41:32 -0500 Return-Path: Via: uk.ac.leicester; Thu, 1 Jul 1993 11:45:11 +0100 Received: from irix.le.ac.uk by xtelpp.leicester.ac.uk with SMTP (PP) id <02472-0@xtelpp.leicester.ac.uk>; Thu, 1 Jul 1993 11:44:27 +0100 From: "Dr C. Cane" Message-Id: <3285.9307011044@irix.le.ac.uk> Subject: VoxelView/Mac PICT files To: nih-image@soils.umn.edu Date: Thu, 1 Jul 93 11:44:41 BST X-Mailer: ELM [version 2.3 PL0 (LUT)] I have a problem with importing PICT files generated by VoxelView/Mac into Image - the LUT is always changed so the colours are wrong. These PICTs will open fine in Photoshop (with the right colours). I need to import stacks (rotation series) into Image so although I could process each frame through Photoshop this is rather tedious. Is these any way I could get round this? Has anyone else any experience with VoxelView/Mac? Chris Cane, Dept. of Biochemistry, University of Leicester, LEICESTER, LE17RH, U.K. (crc@leicester.ac.uk) From herro001@staff.tc.umn.edu Thu Jul 1 02:24:52 1993 Received: from staff.tc.umn.edu by nx1.soils.umn.edu (5.65c) id AA10569; Thu, 1 Jul 1993 07:20:49 -0500 Return-Path: Message-Id: <199307011220.AA10569@nx1.soils.umn.edu> Received: from rash.med.umn.edu by staff.tc.umn.edu id SMTP-0012c32d78f006597; Thu, 1 Jul 93 07:24:51 -0500 From: "Mike Herron" Reply-To: "Mike Herron" To: nih-image@soils.umn.edu Subject: Photo CD Date: Thu, 1 Jul 93 07:24:52 -0500 I am curious about the Photo CD processing by KODAK. Is it possible to grab Photo CD images from a mac application and "squirt" them into NIH Image? If this is possible...is it wise? The images must be rather large, and I am wondering if the KODAK processing company might "improve" the images in some manner so as to make them inapppropriate for measurement. Mike Herron, Uof MN, Dept. of Deramatology herro001@staff.tc.umn.edu From Espen.Ore@hd.uib.no Thu Jul 1 16:22:07 1993 Received: from alf.uib.no by nx1.soils.umn.edu (5.65c) id AA10854; Thu, 1 Jul 1993 08:17:56 -0500 Return-Path: Received: from gonzo.hd.uib.no by alf.uib.no with SMTP (PP) id <25295-0@alf.uib.no>; Thu, 1 Jul 1993 15:18:20 +0200 Date: Thu, 1 Jul 1993 15:22:07 +0100 To: nih-image From: Espen.Ore@hd.uib.no Subject: Re: Photo CD Message-Id: <"alf.uib.no.323:01.06.93.13.18.29"@uib.no> >I am curious about the Photo CD processing by KODAK. Is it possible to grab >Photo CD images from a mac application and "squirt" them into NIH Image? > With QuickTime and Photo CD extensions installed the images on a photo CD appear as ordinary color (RGB) PICT-files to Mac-applications. >If this is possible...is it wise? The images must be rather large, and I am >wondering if the KODAK processing company might "improve" the images in some >manner so as to make them inapppropriate for measurement. > KODAK uses a proprietary (lossy) compression scheme. Espen Ore Norwegian Computing Centre for the Humanities Espen.Ore@hd.uib.no From John_Mansfield@mse.engin.umich.edu Thu Jul 1 08:36:38 1993 Received: from mse.engin.umich.edu by nx1.soils.umn.edu (5.65c) id AA11022; Thu, 1 Jul 1993 08:36:38 -0500 Return-Path: Message-Id: <199307011336.AA11022@nx1.soils.umn.edu> Date: 1 Jul 1993 09:20:54 U From: "John Mansfield" Subject: Re: Photo CD To: nih-image@soils.umn.edu Reply_ RE>Photo CD Reply from: John Mansfield North Campus Electron Microbeam Analysis Laboratory University of Michigan 2455 Hayward Ann Arbor MI 48109-2143 Phone: (313)936-3352 jfmjfm@engin.umich.edu or John_Mansfield@mse.engin.umich.edu Yes it is, there is an Adobe Photoshop Plug-In from Kodak (I believe) that does just that. I have the Kodak PhotoCD Acquire Module v6.4. I dont know if I can give it away though, I will ask Kodak and if I can I will upload it to zippy, otherwise you may have to buy it. It came with a load of Kodak stuff we were trying out. -------------------------------------- Date: 7/1/93 8:27 To: John Mansfield From: nih-image@soils.umn.edu I am curious about the Photo CD processing by KODAK. Is it possible to grab Photo CD images from a mac application and "squirt" them into NIH Image? If this is possible...is it wise? The images must be rather large, and I am wondering if the KODAK processing company might "improve" the images in some manner so as to make them inapppropriate for measurement. Mike Herron, Uof MN, Dept. of Deramatology herro001@staff.tc.umn.edu ------------------ RFC822 Header Follows ------------------ Received: by mse.engin.umich.edu with SMTP;1 Jul 1993 08:26:58 U Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c32d7b5007417; Thu, 1 Jul 93 07:25:26 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA10592; Thu, 1 Jul 1993 07:21:00 -0500 Date: Thu, 1 Jul 1993 07:21:00 -0500 Message-Id: <199307011220.AA10569@nx1.soils.umn.edu> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: "Mike Herron" To: Multiple recipients of list Subject: Photo CD From glenmac@u.washington.edu Thu Jul 1 10:24:15 1993 Received: from carson.u.washington.edu by nx1.soils.umn.edu (5.65c) id AA13805; Thu, 1 Jul 1993 19:27:21 -0500 Return-Path: Received: by carson.u.washington.edu (5.65/UW-NDC Revision: 2.22 ) id AA11116; Thu, 1 Jul 93 17:31:23 -0700 Date: Thu, 1 Jul 1993 17:24:15 -0700 (PDT) From: Glen Macdonald Sender: Glen Macdonald Reply-To: Glen Macdonald Subject: Circularity To: nih-image@soils.umn.edu Cc: Multiple recipients of list In-Reply-To: <9306222123.AA26691@pennsy.med.jhu.edu> Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=us-ascii What is the best way to computer the circularity of a cell? Traditionally, our lab has always used the ratio of minor to major axes. However the Measurements Macro file that Wayne includes with Image contains the following formula in a cirularity macro. It was contributed by someone who is nolonger around. Any comments? Given the first 2 factors, perhaps this equation is related to sphericity. rUser1[rCount]:=4*3.14159265*(rArea[rCount]/sqr(rLength[rCount])); Thanks. -Glen From pgould@uwovax.uwo.ca Thu Jul 1 17:50:40 1993 Received: from julian.uwo.ca by nx1.soils.umn.edu (5.65c) id AA14136; Thu, 1 Jul 1993 20:51:16 -0500 Return-Path: Received: from hydra.uwo.ca by julian.uwo.ca with SMTP id AA01194; Thu, 1 Jul 93 21:55:11 -0400 From: "P.V. GOULD" To: Date: Thu, 1 Jul 93 21:50:40 EDT Subject: Re: Photo CD In-Reply-To: <199307011220.AA10569@nx1.soils.umn.edu> of Thu, 1 Jul 1993 07:25:10 -0500 Message-Id: <0741563440@uwovax.uwo.ca> You can export PhotoCD images as TIFF files using Kodak PhotoCD Access, which costs about $45. You can also crop the image and just export the result. I have made 8 bit greyscale TIFFs of the 1024 x 1536 resolution format and opened them with Image; you just have to set the clipboard buffer to 1500K. Peter Gould, UWO, Department of Pathology From dittmer@fokus.uke.uni-hamburg.de Thu Jul 1 19:23:28 1993 Received: from deneb.dfn.de by nx1.soils.umn.edu (5.65c) id AA14706; Thu, 1 Jul 1993 23:26:30 -0500 Return-Path: Received: from fbihh.informatik.uni-hamburg.de by deneb.dfn.de (4.1/SMI-4.2) id AA07538; Fri, 2 Jul 93 06:30:06 +0200 Message-Id: <9307020430.AA10432@fbihh.informatik.uni-hamburg.de> Received: from fokus.uke.uni-hamburg.de by fbihh.informatik.uni-hamburg.de (5.65+/FBIHH-1.6) with SMTP; id AA10432; Fri, 2 Jul 93 06:30:19 +0200 Date: Thu, 1 Jul 1993 17:23:28 +0200 From: dittmer@fokus.uke.uni-hamburg.de To: nih-image@soils.umn.edu Subject: Two beginners questions ... X-Vms-To: SMTP%"nih-image@soils.umn.edu" Hi Imagers ! (This is my first posting, so please bear with me.) Just starting to use NIHImage, I have two problems : 1) The Tool window shows not the tools shown in the documentation but rather scrambled pieces of black & white pixels. Highlighting the tools and balloon help work ok, as does using the tools. Is there something to be done ? 2) A PICT I'd like to open with NIHImage does always come up in b&w, though it's a colored PICT and displays ok in Canvas and Photoshop. Could there be something I have to adjust ? Many thanks in advance for any help you can supply. Greetings Ulf Dittmer dittmer@uke.uni-hamburg.de From reuber@hydra.rose.brandeis.edu Fri Jul 2 04:56:10 1993 Received: from hydra.rose.brandeis.edu (hydra.cc.brandeis.edu) by nx1.soils.umn.edu (5.65c) id AA16585; Fri, 2 Jul 1993 08:52:12 -0500 Return-Path: Received: by hydra.rose.brandeis.edu (5.57/Ultrix3.0-C) id AA14230; Fri, 2 Jul 93 09:59:04 -0400 Message-Id: <9307021359.AA14230@hydra.rose.brandeis.edu> Date: Fri, 2 Jul 1993 09:56:10 -0500 To: From: reuber@hydra.rose.brandeis.edu (Kent Reuber) Subject: Re: Circularity >What is the best way to computer the circularity of a cell? > >Traditionally, our lab has always used the ratio of minor to major axes. >However the Measurements Macro file that Wayne includes with Image >contains the following formula in a cirularity macro. It was contributed >by someone who is nolonger around. Any comments? > Given the first 2 factors, perhaps this equation is related to >sphericity. > > rUser1[rCount]:=4*3.14159265*(rArea[rCount]/sqr(rLength[rCount])); > This formula derives from the ratio of the area of a circle to the square of the perimerter: A/P^2 = Pi R^2/(4 Pi R^2) = 1/(4 Pi) or 4 Pi A/P^2 = 1 (this ratio is 1 for a circle) This ratio can be fairly small for complicated objects. For example, we found that partially-healed circular wounds in eye tissue can have ratios of 1/5 or 1/7 or less. Kent Reuber, Computational Specialist Biology Department, Brandeis University reuber@hydra.rose.brandeis.edu From wayne@zippy.nimh.nih.gov Fri Jul 2 08:54:42 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA16614; Fri, 2 Jul 1993 08:52:47 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA27823; Fri, 2 Jul 93 09:09:55 -0400 Message-Id: <9307021309.AA27823@zippy.nimh.nih.gov> Date: Fri, 2 Jul 1993 08:54:42 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Two beginners questions ... >1) The Tool window shows not the tools shown in the > documentation but rather scrambled pieces of > black & white pixels. Highlighting the tools > and balloon help work ok, as does using the > tools. Is there something to be done ? The scrambled tools problem is fixed in the 1.50b84 beta available by anonymous ftp from zippy.nimh.nih.gov. >2) A PICT I'd like to open with NIHImage does always > come up in b&w, though it's a colored PICT > and displays ok in Canvas and Photoshop. > Could there be something I have to adjust ? Could I get a copy of one of these troublesome PICT files? You can either upload it to the contrib directory on zippy.nimh.nih.gov or email me a binhexed version. --wayne From jmorrow@pirl.lpl.arizona.edu Fri Jul 2 05:01:58 1993 Received: from Arizona.edu (Merlin.Telcom.Arizona.EDU) by nx1.soils.umn.edu (5.65c) id AA00338; Fri, 2 Jul 1993 14:01:36 -0500 Return-Path: Received: from pirl.lpl.arizona.edu by Arizona.edu (PMDF V4.2-13 #2381) id <01H02EBK3ESG9EDWKT@Arizona.edu>; Fri, 2 Jul 1993 12:03:03 MST Received: by pirl.lpl.arizona.edu (4.1/SMI-3.2 LPL-MCN 1.25 91/08/23) id AA09608; Fri, 2 Jul 93 12:01:58 MST Date: Fri, 02 Jul 1993 12:01:58 -0700 (MST) From: jmorrow@pirl.lpl.arizona.edu (John Morrow) Subject: Averaging Stacks To: nih-image@soils.umn.edu Message-Id: <9307021901.AA09608@pirl.lpl.arizona.edu> Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Is there a command in the macro language that corresponds to the 'Average' command in the 'Stacks' menu? John Morrow Image Processing for Teaching project University of Arizona From 00A0919@msgate.emis.hac.com Fri Jul 2 21:49:38 1993 Received: from hac2arpa.hac.com by nx1.soils.umn.edu (5.65c) id AA03785; Sat, 3 Jul 1993 06:45:28 -0500 Return-Path: <00A0919@msgate.emis.hac.com> Received: by hac2arpa.hac.com (4.1/SMI-DDN) id AA28421; Sat, 3 Jul 93 04:49:38 PDT Date: Sat, 3 Jul 93 04:49:38 PDT From: 00A0919@msgate.emis.hac.com Message-Id: <9307031149.AA28421@hac2arpa.hac.com> Received: by DniMail (v1.0); Sat Jul 3 04:49:27 1993 PDT To: IMAGE@hac2arpa.hac.com, BB@hac2arpa.hac.com Subject: RE: Radius Rocket 33 From: Sumida, David S Date: Fri, Jul 2, 1993 9:50 AM Subject: RE: Radius Rocket 33 To: Image BB >Before I upgrade my MacII with the Rocket33 Accelerator for use with image >analysis I want to determine whether anyone has experience with this >hardware and its compatibility. This above message was put on the Image "bulletin board" several weeks ago but my reply somehow bounced, so I'm sending it again. **************** In partial answer to Robbie Burk's question: As I mentioned earlier in another message to the Image server, I have upgraded my MacII with a Daystar Digital Universal PowerCache 030 running at 50 MHz and have found no incompatibility with software applications thusfar (i.e. Image, Word, Labview, etc). I chose the Daystar over the Rocket33 because of strong recommendations from the computer technical staff here. One of the advantages of the Daystar is that they offer upward compatibility/trade-ins with their newer Turbo 040 products as they become available for the different Mac platforms. David Sumida, PhD 00a0919@msgate.emis.hac.com From RBLYSTON@VM1.TUCC.TRINITY.EDU Sat Jul 3 09:59:19 1993 Received: from vm1.tucc.trinity.edu by nx1.soils.umn.edu (5.65c) id AA05337; Sat, 3 Jul 1993 14:58:29 -0500 Return-Path: Message-Id: <199307031958.AA05337@nx1.soils.umn.edu> Received: from VM1.tucc.trinity.edu by VM1.TUCC.TRINITY.EDU (IBM VM SMTP V2R1) with BSMTP id 5676; Sat, 03 Jul 93 15:01:51 CDT Received: from TRINITY.EDU (RBLYSTON) by VM1.tucc.trinity.edu (Mailer R2.10 ptf000) with BSMTP id 6500; Sat, 03 Jul 93 15:01:50 CDT Date: Sat, 03 Jul 93 14:59:19 CDT From: Robert Blystone Subject: MRI scans To: nih-image group Hello to all: I am looking for MRI scans of rats. I need to build a 3-D model of a rat and map temperature changes on the animal. Can anyone out there direct me to an MRI file of a whole rat. Thanks. Blystone in Texas R. Blystone Biology Trinity University San Antonio RBLYSTON@Trinity.edu ********************** ROBERT V. BLYSTONE PHONE:(210)736-7243 DEPARTMENT OF BIOLOGY FAX:(210)736-7229 Trinity University E-Mail:RBlyston@Trinity.edu 715 Stadium Drive San Antonio, TX, 78212 From morilak@cmgm.stanford.edu Mon Jul 5 05:23:10 1993 Received: from cmgm.Stanford.EDU by nx1.soils.umn.edu (5.65c) id AA14145; Mon, 5 Jul 1993 14:14:11 -0500 Return-Path: Received: from (P159-macclassic.Stanford.EDU) by cmgm.stanford.edu (4.1/1.34) id AA13697; Mon, 5 Jul 93 12:18:17 PDT Date: Mon, 5 Jul 1993 12:23:10 PDT From: YOUR NAME HERE Subject: Rocket etc accelerators To: nih-image@soils.umn.edu Message-Id: This will be a very naive question, but in discussion of the Radius Rocket 33 and alternatives, are we talking about CPU upgrades, or is this strictly a video accelerator? Is it a new CPU, or is it a plug-in board (as you might be able to tell, my history is with PCs!)? Is a video accelerator advisable for using Image? Thanks! David Morilak Dept Psychiatry Stanford Univ morilak@cmgm.stanford.edu ------- From wayne@zippy.nimh.nih.gov Tue Jul 6 08:58:14 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA17969; Tue, 6 Jul 1993 08:56:16 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA01498; Tue, 6 Jul 93 09:12:02 -0400 Message-Id: <9307061312.AA01498@zippy.nimh.nih.gov> From: wayne@helix.nih.gov(Wayne Rasband) To: Subject: Re: Rocket etc accelerators Date: Tue, 6 Jul 1993 08:58:14 +0000 >This will be a very naive question, but in discussion of the Radius Rocket 33 >and alternatives, are we talking about CPU upgrades, or is this strictly a >video accelerator? Is it a new CPU, or is it a plug-in board (as you might be >able to tell, my history is with PCs!)? Is a video accelerator advisable for >using Image? Thanks! The Radius Rocket 33 is CPU upgrade the replaces the 68030 CPU in a Mac II with a faster 68040 CPU. A video(QuickDraw) accelerator is probably a waste of money if you primarily use NIH Image. Instead, to get the fastest animation and scrolling, use the built-in video available an all recent Macs. Built-in video avoids the "NuBus bottleneck" by providing a higher speed path between the CPU and video memory(VRAM). The LC III supports up to 16 inch monitors and the Centris 610, Centris 650 and all Quadras support up to 21 inch monitors. --wayne From wayne@zippy.nimh.nih.gov Tue Jul 6 08:58:16 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA17971; Tue, 6 Jul 1993 08:56:17 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA01500; Tue, 6 Jul 93 09:12:03 -0400 Message-Id: <9307061312.AA01500@zippy.nimh.nih.gov> Date: Tue, 6 Jul 1993 08:58:16 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Rocket etc accelerators >This will be a very naive question, but in discussion of the Radius Rocket 33 >and alternatives, are we talking about CPU upgrades, or is this strictly a >video accelerator? Is it a new CPU, or is it a plug-in board (as you might be >able to tell, my history is with PCs!)? Is a video accelerator advisable for >using Image? Thanks! The Radius Rocket 33 is CPU upgrade the replaces the 68030 CPU in a Mac II with a faster 68040 CPU. A video(QuickDraw) accelerator is probably a waste of money if you primarily use NIH Image. Instead, to get the fastest animation and scrolling, use the built-in video available an all recent Macs. Built-in video avoids the "NuBus bottleneck" by providing a higher speed path between the CPU and video memory(VRAM). The LC III supports up to 16 inch monitors and the Centris 610, Centris 650 and all Quadras support up to 21 inch monitors. --wayne From mvivino@helix.nih.gov Tue Jul 6 07:22:46 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA18577; Tue, 6 Jul 1993 10:18:41 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA24840; Tue, 6 Jul 93 11:22:46 -0400 Date: Tue, 6 Jul 93 11:22:46 -0400 Message-Id: <9307061522.AA24840@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: SelectWindow('Title') >From the change history: 22) A command("SelectWindow('Title')") was added to the macro language for selecting windows my name. Since this was talked about on the list about a month ago, I see that wayne has added this command. Thanks wayne, that should make working with many images in a macro easier. This because if we open and close multiple images sometimes the image number changes and makes things difficult. Much better now! Mark Vivino NIH/DCRT mvivino@helix.nih.gov From huff@MCCLB0.MED.NYU.EDU Tue Jul 6 14:04:20 1993 Received: from mcclb0.med.nyu.edu by nx1.soils.umn.edu (5.65c) id AA20773; Tue, 6 Jul 1993 18:00:29 -0500 Return-Path: Received: from [128.122.133.136] (PGL6.CHEM.NYU.EDU) by MCCLB0.MED.NYU.EDU (PMDF V4.2-13 #2884) id <01H08E796I340000HX@MCCLB0.MED.NYU.EDU>; Tue, 6 Jul 1993 19:04:22 EDT Message-Id: <01H08E79FCDE0000HX@MCCLB0.MED.NYU.EDU> X-Envelope-To: nih-image@soils.umn.edu From: huff@MCCLB0.MED.NYU.EDU (Edward J. Huff) To: nih-image@soils.umn.edu Subject: Re: SelectWindow('Title') Date: Tue, 06 Jul 1993 19:04:20 -0500 Content-Transfer-Encoding: 7BIT >>From the change history: > >22) A command("SelectWindow('Title')") was added to the macro language for >selecting windows my name. > >Since this was talked about on the list about a month ago, I see that wayne >has added this command. Thanks wayne, that should make working with many >images in a macro easier. This because if we open and close multiple images >sometimes the image number changes and makes things difficult. Much better >now! > >Mark Vivino >NIH/DCRT >mvivino@helix.nih.gov This is not an optimum solution, since sometimes two windows have the same name. If you are trying to write bullet-proof macros, this isn't quite good enough. I discussed a change with Wayne back then, but didn't see an answer to his objection toward confusing users with another kind of window number. I figured out an answer in the meantime: define a sticky picnumber function picSerial which gives a serial number of the pic window. The first pic window opened has picSerial of say 65536 (or 100000 if you think that number is "rounder") and selectPic will accept either kind of pic number. The serial numbers are never reused, and never change even if pics are disposed, so you can note the number at the beginning of a macro and select it later without worrying if it is a different image. Maybe there should also be a picExists function which works with either picNumber or picSerial, returns true or false, so you can check to see if the window is around and put up a useful dialog box (or recreate the window) instead of just giving a macro error. BTW are global macro variables documented? I found out about them while looking at the code. When you use a macro global to note a pic number, you REALLY have trouble with numbers changing. It can happen if the user closes the window you noted, or any older window. -- Edward J. Huff huff@mcclb0.med.nyu.edu (212)998-8465 Keck Laboratory for Biomolecular Imaging NYU Chemistry Deptartment, 31 Washington Place, New York NY 10003 From cls7@cornell.edu Tue Jul 6 15:19:58 1993 Received: from POSTOFFICE.MAIL.CORNELL.EDU by nx1.soils.umn.edu (5.65c) id AA20907; Tue, 6 Jul 1993 18:15:57 -0500 Return-Path: Received: from [132.236.236.86] (CU-DIALUP-0312.CIT.CORNELL.EDU) by postoffice.mail.cornell.edu with SMTP id AA02846 (5.65c8/IDA-1.4.4 for nih-image@nx1.soils.umn.edu); Tue, 6 Jul 1993 19:19:58 -0400 Date: Tue, 6 Jul 1993 19:19:58 -0400 Message-Id: <199307062319.AA02846@postoffice.mail.cornell.edu> To: nih-image@soils.umn.edu From: cls7@cornell.edu (Carl Schofield) X-Sender: cls7@postoffice.mail.cornell.edu Subject: DEM Macro I am looking for an Image macro that will import USGS DEM (Digital Elevation Model) files, in a manner similar to the import text function built into Image. Please advise as to availability of such a macro. From norm_hurst@maca.sarnoff.com Wed Jul 7 08:25:20 1993 Received: from nova (sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA23692; Wed, 7 Jul 1993 08:25:20 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA17110; Wed, 7 Jul 93 09:29:10 EDT Message-Id: <9307071329.AA17110@nova> Date: 7 Jul 1993 09:28:17 U From: "Norm Hurst" Subject: global macro vars? To: "NIH Image user group" global macro vars? 7/7/93 9:21 AM >>> BTW are global macro variables documented? Does this mean I can remember numbers and strings between macro invokations? What's the syntax? -Norm Hurst David Sarnoff Research Center From wayne@zippy.nimh.nih.gov Wed Jul 7 08:39:55 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA23836; Wed, 7 Jul 1993 08:37:58 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA03313; Wed, 7 Jul 93 08:53:48 -0400 Message-Id: <9307071253.AA03313@zippy.nimh.nih.gov> Date: Wed, 7 Jul 1993 08:39:55 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: global macro vars? >global macro vars? 7/7/93 > 9:21 AM >>>> BTW are global macro variables documented? > >Does this mean I can remember numbers and strings between macro invokations? > >What's the syntax? Global variables are listed at the beginning of the macro file. I have attached an example macro file that uses global variables. --wayne ----------------------------------------- var gvar1,gvar2,gvar3:integer; gstring:string; macro 'Macro 1 [1]'; begin gvar1:=GetNumber('gvar1:',gvar1) end; macro 'Macro 2 [2]'; begin gvar1:=GetNumber('gvar1:',gvar1) end; From norm_hurst@maca.sarnoff.com Wed Jul 7 09:42:50 1993 Received: from nova (sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA24169; Wed, 7 Jul 1993 09:42:50 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA17388; Wed, 7 Jul 93 10:46:51 EDT Message-Id: <9307071446.AA17388@nova> Date: 7 Jul 1993 10:45:03 U From: "Norm Hurst" Subject: Chroma "crosshatch" filter To: "NIH Image user group" Chroma "crosshatch" filter 7/7/93 10:28 AM There's been some discussion about a crosshatch pattern that appears in images digitized from composite color cameras. Here is a set of convolution tap weights that will notch out the color subcarrier. Put them in a text file and use that file with the "Enhance/Convolve..." menu. These tap weights assume that you are sampling at 4 times the frequency of the color subcarrier (the crosshatch repeats every fourth sample horizontally). 0 0 0 0 0 -1 0 2 0 -1 2 0 12 0 2 -1 0 2 0 -1 0 0 0 0 0 The above taps assume that you have grabbed a single NTSC "field" (1/60th of a sec) and that the color crosshatch alternates polarity by lines. If you have a pair of fields laced into a frame (the crosshatch alternates every OTHER line), use these taps: -1 0 2 0 -1 0 0 0 0 0 2 0 12 0 2 0 0 0 0 0 -1 0 2 0 -1 Both of these filters remove some of the luminance info. If your frame grabber samples at a rate other than 4 times subcarrier these won't work so well. If that's the case, let me know and I'll see if I can help. If you are using a PAL camera, you will need yet different taps. Your best bet is to shut off the color from the camera, or use a b/w camera. -Norm Hurst David Sarnoff Research Center From gibbons@trix.genie.uottawa.ca Wed Jul 7 07:40:10 1993 Received: from trix.genie.uottawa.ca by nx1.soils.umn.edu (5.65c) id AA24487; Wed, 7 Jul 1993 10:36:42 -0500 Return-Path: Received: by trix.genie.uottawa.ca (5.57/Ultrix3.0-C) id AA18389; Wed, 7 Jul 93 11:40:10 -0400 Date: Wed, 7 Jul 93 11:40:10 -0400 Message-Id: <9307071540.AA18389@trix.genie.uottawa.ca> To: nih-image@soils.umn.edu From: gibbons@trix.genie.uottawa.ca Subject: 3D data I am looking for 3D data of normal humans. Anybody know where I can find any files or CAT scans suitable for parsing. David From Kardon-Randy@ophthalmology-po.ophth.uiowa.edu Sun Jul 7 08:05:00 1993 Received: from ns-mx.uiowa.edu by nx1.soils.umn.edu (5.65c) id AA25509; Wed, 7 Jul 1993 14:15:55 -0500 Return-Path: Received: from argos.weeg.uiowa.edu by ns-mx.uiowa.edu (5.64.jnf/920408) on Wed, 7 Jul 93 14:20:18 -0500 id AA03218 with SMTP From: Received: by argos.weeg.uiowa.edu (5.64.jnf/921215) on Wed, 7 Jul 93 14:20:15 -0500 id AA04144 Message-Id: <9307071920.AA04144@argos.weeg.uiowa.edu> Received: by ccmail-gateway.uiowa.edu for cc:Mail translation to SMTP on Wed Jul 07 14:19:37 1993 To: gibbons%trix.genie.uottawa.ca@relay.tc.umn.edu, Multiple recipients of list Date: 7 Jul 93 14:05 CST Subject: 3D data In reply to your request for 3D data on humans, Dr Hanna Damasio, Dept Neurology, University of Iowa Hospital,Iowa City, Iowa (319-356-0450) is studying the 3D reconstruction of the human brain using voxel collected MRIs. I would recommend contacting her. Yours truly, Randy Kardon MD PhD Neuro-ophthalmology Division Ophthalmology Dept Univ of Iowa Hospitals From NJOHNSON@ubvms.cc.buffalo.edu Sun Jul 7 11:37:25 1993 Received: from ubvmsc.cc.buffalo.edu by nx1.soils.umn.edu (5.65c) id AA25661; Wed, 7 Jul 1993 14:30:40 -0500 Return-Path: Received: from ubvms.cc.buffalo.edu by ubvms.cc.buffalo.edu (PMDF #2452 ) id <01H09KV5ZFDI8ZFP6P@ubvms.cc.buffalo.edu>; Wed, 7 Jul 1993 15:37:25 EDT Date: 07 Jul 1993 15:37:25 -0400 (EDT) From: NJOHNSON@ubvms.cc.buffalo.edu Subject: WANTEDD: CAT scan of mandible for NIH Image 1.43 To: nih-image@soils.umn.edu Message-Id: <01H09KV5ZFDK8ZFP6P@ubvms.cc.buffalo.edu> Organization: University at Buffalo X-Vms-To: IN%"nih-image@soils.umn.edu" X-Vms-Cc: NJOHNSON Mime-Version: 1.0 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT I am working on a National Institutes of Health (NIH) grant on bone growth and need two CAT scan series from the archives of a living child who has grown a couple of years inbetween scans. The CAT scans would be of a child's mandible showing growth over a period of one to three years. If the child aged from 6 years old to 8 years old, that would be ideal, but other age ranges would be very useful,too. I will be analyzing the images in the software program "NIH Image 1.43." Any help on directing me towards a source of such CAT scans would be greatly appreciated. Noreen Johnson njohnson@ubvms.cc.buffalo.edu From mvivino@helix.nih.gov Thu Jul 8 06:47:10 1993 Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA00552; Thu, 8 Jul 1993 09:42:51 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA29899; Thu, 8 Jul 93 10:47:10 -0400 Date: Thu, 8 Jul 93 10:47:10 -0400 Message-Id: <9307081447.AA29899@helix.nih.gov> To: NIH-IMAGE@SOILS.UMN.EDU From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: new 'Find' command >From change history: 26) A Find command was added to the Edit menu. It replaces Fill whenever a text window is active. Type Option-Command-F to repeatedly search for the same string Pretty soon the macros will be just like think pascal!!! Lets see, some day you could have macros creating pascal code resources, and pascal creating and executing macros. Maybe that means a life form has been created. Mark Vivino NIH/DCRT mvivino@helix.nih.gov From cammer@alsys1.aecom.yu.edu Thu Jul 8 13:57:20 1993 Received: from alsys1.aecom.yu.edu by nx1.soils.umn.edu (5.65c) id AA01898; Thu, 8 Jul 1993 16:57:15 -0500 Return-Path: Received: by alsys1.aecom.yu.edu id AA11630 (5.65c/IDA-1.4.4/AECOM-RIT for nih-image@soils.umn.edu); Thu, 8 Jul 1993 18:01:39 -0400 In-Reply-To: <9307071253.AA03313@zippy.nimh.nih.gov> Message-Id: Mime-Version: 1.0 From: Michael Cammer To: nih-image@soils.umn.edu Subject: Time lapse VRC query Date: Thu, 8 Jul 1993 17:57:20 -0400 (EDT) Content-Type: TEXT/PLAIN; charset=US-ASCII We have been having difficulties digitizing single frames from a Panasonic AG-6720 time lapse video recorder because the top of each frame is not always in register with the frames before or after or because the interlacing is not matched. Does anybody know of a better time lapse VCR or other solution to this problem for under $2500? Thanks- Michael cammer@aecom.yu.edu From SQUIDNEY@uncvx1.oit.unc.edu Sun Jul 11 11:10:53 1993 Received: from uncvx1.oit.unc.edu by nx1.soils.umn.edu (5.65c) id AA03978; Sun, 11 Jul 1993 15:03:12 -0500 Return-Path: Received: from uncvx1.oit.unc.edu by uncvx1.oit.unc.edu (PMDF V4.2-11 #3863) id <01H0F7ICJ2200013J5@uncvx1.oit.unc.edu>; Sun, 11 Jul 1993 16:10:53 EST Message-Id: <01H0F7ICJ2220013J5@uncvx1.oit.unc.edu> X-Vms-To: IN%"nih-image@soils.umn.edu" Mime-Version: 1.0 From: SQUIDNEY@uncvx1.oit.unc.edu To: nih-image@soils.umn.edu Subject: Image Processing Texts Date: Sun, 11 Jul 1993 16:10:53 -0500 (EST) Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-Transfer-Encoding: 7BIT Several weeks ago I asked this group for digital image processing texts which might bring a little depth to those of us who wish to learn more about the subject. Below are the several texts most suggested by those on the NIH-Image mailing list and several additional references dealing more directly with microscopy applications. I wish to thank all who answered my query and hope this is of some use to others in the field. Inoue, S. 1986. Video Microscopy. Plenum Press. Russ, JC. 1992. The Image Processing handbook. CRC Press. Gonzalez, RC and RE Woods. 1992. Digital image processing. Addison-Wesley Publishing Company. Russ, JC. 1990. Computer-Assisted Microscopy. Plenum Press, New York. Pratt, WK. 1978. Digital Image Processing. John Wiley & Sons. Slayter, EM and Slayter, HS. 1992. Light and Electron Microscopy. Cambridge University Press. Visualization in Biomedical Microscopies. 1992. Ed. Kriete, A. VCH Publishers. Sidney L. Shaw UNC-Chapel Hill From cyohn@scripps.edu Mon Jul 12 07:21:51 1993 Received: from scripps.edu (riscsm.scripps.edu) by nx1.soils.umn.edu (5.65c) id AA08220; Mon, 12 Jul 1993 16:20:46 -0500 Return-Path: Received: from terrapin.scripps.edu by scripps.edu (5.61/1.34) id AA17452; Mon, 12 Jul 93 14:21:53 -0700 Received: by terrapin.Scripps.EDU (4.1/SMI-4.1) id AA11098; Mon, 12 Jul 93 14:21:51 PDT Date: Mon, 12 Jul 93 14:21:51 PDT From: cyohn@scripps.edu (Christopher Yohn) Message-Id: <9307122121.AA11098@terrapin.Scripps.EDU> To: nih-image@soils.umn.edu Subject: Application: DNA gels I am setting up a system based on NIH-Image for some simple applications, primarily DNA agarose gels. I have encountered two problems and am seeking recommendations. 1) I need a UV filter for the camera that will filter the light from the UV light box used to visualize the DNA. Most standard photographic filters are not adequate. Any suggestions will be appriciated. 2) I've experienced inconsistent results when using the "Sum Frames" option under Average Frames... in the Special menu. This is often neccessary to visualize faint bands, and it seems to work well, but only randomly about 30% of the time. Other times it seems to look no different than live capture mode. I haven't been able to determine why it works some of the time and not others. Any ideas? (Note: I'm using v1.47 which I obtained from Scion because I'm using their TV-3 card. This is the only version I have which has the "Print Video" which accesses that card.) While I'm on the subject of the TV-3, are future versions of NIH-Image going to include the Print Video option, or am I going to have to rely on Scion to get updated versions? Thanks for any info you can provide. Chris Yohn cyohn@scripps.edu The Scripps Research Institute From SMASESM@UMSLVMA.UMSL.EDU Tue Jul 13 03:39:21 1993 Received: from umslvma.umsl.edu by nx1.soils.umn.edu (5.65c) id AA12403; Tue, 13 Jul 1993 08:46:15 -0500 Return-Path: Message-Id: <199307131346.AA12403@nx1.soils.umn.edu> Received: from UMSLVMA by UMSLVMA.UMSL.EDU (IBM VM SMTP V2R2) with BSMTP id 6195; Tue, 13 Jul 93 08:51:08 CDT Received: from UMSLVMA (SMASESM) by UMSLVMA (Mailer R2.08) with BSMTP id 6252; Tue, 13 Jul 93 08:51:08 CDT Date: Tue, 13 Jul 93 08:39:21 CDT From: Mike Sesma Subject: Re: new 'Find' command To: nih-image@soils.umn.edu In-Reply-To: Message of Tue, 13 Jul 1993 07:10:59 -0500 from Which version of IMAGE has the new Find command or can I append the routine to ver 1.49? I am also having trouble with the Set Scale command under the Analysis Menu. If I double-click on the line tool the dialog refers to the pixel width of the line. If I use the line tool to trace a known distance then bring up the Set Scale (e.g., measure 100 microns) the box for actual distance and pixel distance do not reflect the actions of the line tool. According to the 1.49 documentation I am not doing anything wrong. I have worked around this problem but I would rather be able to set scale directly. Any suggestions or corrections to this procedure? Thanks. Mike Sesma University of Missouri-St. Louis School of Optometry SMASESM @ UMSLVMA.UMSL.EDU 314-553-5995 From Charles.P.Daghlian@Dartmouth.EDU Sat Jul 13 05:52:38 1993 Received: from dartvax.dartmouth.edu by nx1.soils.umn.edu (5.65c) id AA12409; Tue, 13 Jul 1993 08:48:16 -0500 Return-Path: Received: from donner.dartmouth.edu by dartvax.dartmouth.edu (5.65+D5/4.5HUB) id AA26739; Tue, 13 Jul 93 09:52:40 -0400 Message-Id: <5966918@donner.Dartmouth.EDU> Date: 13 Jul 93 09:52:38 EDT From: Charles.P.Daghlian@Dartmouth.EDU (Charles P. Daghlian) Subject: undeliverable mail messages To: nih-image@soils.umn.edu Would you please change your mail system so we stop getting the many undeliverable mail messages. If this is not possible, please remove me from the list. From wayne@zippy.nimh.nih.gov Tue Jul 13 09:52:07 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA13282; Tue, 13 Jul 1993 09:49:53 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA13114; Tue, 13 Jul 93 10:06:35 -0400 Message-Id: <9307131406.AA13114@zippy.nimh.nih.gov> Date: Tue, 13 Jul 1993 09:52:07 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Scion TV-3 >While I'm on the subject of the TV-3, are future versions of NIH-Image >going to include the Print Video option, or am I going to have to >rely on Scion to get updated versions? Tod Weinberg of Scion is working on a Photoshop compatible export plug-in for the TV-3 that will work with Image 1.50. For those not familiar with it, the TV-3 is an RS-170 output board useful for getting quick hardcopy of images using a video printer. --wayne From dmorris@bmrl.med.uiuc.edu Tue Jul 13 05:15:09 1993 Received: from bmrl.med.uiuc.edu by nx1.soils.umn.edu (5.65c) id AA13526; Tue, 13 Jul 1993 10:11:45 -0500 Return-Path: Received: by bmrl.med.uiuc.edu id AA01085 (5.67a/IDA-1.4.2 for nih-image@soils.umn.edu); Tue, 13 Jul 1993 10:15:09 -0500 From: Doug Morris Message-Id: <199307131515.AA01085@bmrl.med.uiuc.edu> Subject: Re: Undeliverable Mail To: nih-image@soils.umn.edu Date: Tue, 13 Jul 1993 10:15:09 -0500 (CDT) In-Reply-To: <199307081949.AA01499@nx1.soils.umn.edu> from "SMTP@cc1.kuleuven.ac.be" at Jul 13, 93 07:13:48 am X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 79 Please put a filter on listserv to avoid massed amounts of undeliverable mail. From wayne@zippy.nimh.nih.gov Tue Jul 13 10:19:09 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA13577; Tue, 13 Jul 1993 10:16:55 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA13206; Tue, 13 Jul 93 10:33:38 -0400 Message-Id: <9307131433.AA13206@zippy.nimh.nih.gov> Date: Tue, 13 Jul 1993 10:19:09 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: new 'Find' command >Which version of IMAGE has the new Find command or can I append the routine to >ver 1.49? The Find command is in the 1.50b95 beta version available by anonymous FTP From zippy.nimh.nih.gov. Received: from cbis.ece.drexel.edu by nx1.soils.umn.edu (5.65c) id AA13675; Tue, 13 Jul 1993 10:21:03 -0500 Return-Path: Received: from homer.ece.drexel.edu by cbis.ECE.Drexel.EDU (5.67/1.34) id AA28509; Tue, 13 Jul 93 11:25:15 EDT Message-Id: <9307131525.AA28509@cbis.ECE.Drexel.EDU> Date: 13 Jul 1993 11:17:33 U From: "Carl Gustafson" Subject: Mailer Rejection Slips To: " " Subject: Time:11:13 AM OFFICE MEMO Mailer Rejection Slips Date:7/13/93 Lately, it seems that any time someone posts a message to the list, I get a ton of rejection slips from various mail systems. These seem to come from the Listserv. Since I haven't posted anything recently, and the rejections are from other people's messages, I can't help but wonder if something has gone wrong here. (Or is my mailer / am I brain-dead?) Carl Gustafson Puzzled in Philadelphia From xuan@hpsrjtc.sr.hp.com Tue Jul 13 01:51:02 1993 Received: from relay.hp.com by nx1.soils.umn.edu (5.65c) id AA14042; Tue, 13 Jul 1993 10:47:19 -0500 Return-Path: Received: from hpmwtd.sr.hp.com by relay.hp.com with SMTP (16.6/15.5+IOS 3.13) id AA01898; Tue, 13 Jul 93 08:51:45 -0700 Received: from hpnmxmx.sr.hp.com by hpmwtd.sr.hp.com with SMTP (15.11.1.6/15.5+IOS 3.22+OM) id AA28691; Tue, 13 Jul 93 08:51:44 -0700 Received: by hpsrjtc.sr.hp.com (1.37.109.4/15.5+IOS 3.22) id AA05667; Tue, 13 Jul 93 08:51:03 -0700 From: Xiaohua Xuan Message-Id: <9307131551.AA05667@hpsrjtc.sr.hp.com> Subject: Re: undeliverable mail messages To: nih-image@soils.umn.edu () Date: Tue, 13 Jul 93 8:51:02 PDT In-Reply-To: <5966918@donner.Dartmouth.EDU>; from "Charles P. Daghlian" at Jul 13, 93 9:25 am Mailer: Elm [revision: 70.85] You mean that you get something back when listsev send me anything. Could you send me the whole message you got so that I figure out what is wrong. Thank you. --Xiaohua From geller@synapse.umdnj.edu Tue Jul 13 08:01:27 1993 Received: from synapse.UMDNJ.EDU by nx1.soils.umn.edu (5.65c) id AA14280; Tue, 13 Jul 1993 11:02:29 -0500 Return-Path: Date: Tue, 13 Jul 93 12:01:27 EDT From: "Herbert M. Geller" To: nih-image@soils.umn.edu Subject: Export Plug-Ins Message-Id: <9307131201.aa03051@synapse.umdnj.edu> I have just ordered a Photoshop Plug-In for my Kodak 7700 Printer. Will this work with Image? Herb Geller From wayne@zippy.nimh.nih.gov Tue Jul 13 11:30:15 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA14512; Tue, 13 Jul 1993 11:28:04 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA13393; Tue, 13 Jul 93 11:44:43 -0400 Message-Id: <9307131544.AA13393@zippy.nimh.nih.gov> Date: Tue, 13 Jul 1993 11:30:15 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: Export Plug-Ins >I have just ordered a Photoshop Plug-In for >my Kodak 7700 Printer. Will this work with >Image? I haven't tried it, but it should work as long as it's not from Adobe. Adobe designs their plug-ins so they only work with Photoshop. You should use v1.50b96 or later of Image, which I will be uploading later today. It fixes a bug I just found in the support for export plug-ins. --wayne From SMASESM@UMSLVMA.UMSL.EDU Tue Jul 13 06:57:30 1993 Received: from umslvma.umsl.edu by nx1.soils.umn.edu (5.65c) id AA14803; Tue, 13 Jul 1993 11:56:21 -0500 Return-Path: Message-Id: <199307131656.AA14803@nx1.soils.umn.edu> Received: from UMSLVMA by UMSLVMA.UMSL.EDU (IBM VM SMTP V2R2) with BSMTP id 6310; Tue, 13 Jul 93 12:01:14 CDT Received: from UMSLVMA (SMASESM) by UMSLVMA (Mailer R2.08) with BSMTP id 6972; Tue, 13 Jul 93 12:01:13 CDT Date: Tue, 13 Jul 93 11:57:30 CDT From: Mike Sesma Subject: Re: new 'Find' command To: Wayne In-Reply-To: Message of Tue, 13 Jul 1993 09:17:35 -0500 from Wayne, You were correct as usual, I had chosen the incorrect tool. It works fine now. On the issue of the new "find" command and ver 1.50bxx. I was going to wait until the'b'was removed from 1.5 before I downloaded the program. Are there other good reasons for using 1.50bx instead of 1.49 or can I continue to get by without updating? Thanks, Mike Sesma. From sarmienu@rnisd0.DNET.roche.com Tue Jul 13 10:53:35 1993 Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA15895; Tue, 13 Jul 1993 13:49:19 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA04353; Tue, 13 Jul 93 14:53:36 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA28696; Tue, 13 Jul 93 14:42:14 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9307131842.AA28696@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Tue, 13 Jul 93 14:53:35 EDT Date: Tue, 13 Jul 93 14:53:35 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: crashes on image 1.49 I am having continuous crashes with the program Image 1.49 that happen even when I am not touching the computer keyboard or mouse. My technician reports the same problem. I appears to occur when I am at the microscope making adjustments in the light and/or focus. Perhaps a saturation occurs just before the crash. Occasionally crashes the entire system but more often is the Image application that quits with an error of the type 1. I am using Image on a Quadra 950 with Scion LG-3. The version 1.47 works flawlessly. I have been reading about Image 1.50b and others. Does the b stand for beta?, if so, when could we expect a bug free Image 1.50? is it "risky" to use 1.50b? should I wait to download the final version? Could anybody out there discuss resolution with NIH-Image, how to get publication quality photo prints, say via Photoshop or Persuasion? Does any body know of somebody which would do this job at a reasonable price? Juan I. Sarmiento Department of Toxicology and Pathology Hoffmann-La Roche, INC. 340 Kingsland street Nutley, NJ 07110 (201) 235 43 42 Sarmienu@RNISD0.DNET.ROCHE.COM From jladwig@soils.umn.edu Tue Jul 13 09:02:12 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA15967; Tue, 13 Jul 1993 13:59:05 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA08318; Tue, 13 Jul 93 14:02:12 CDT Message-Id: <9307131902.AA08318@saturn.soils.umn.edu> In-Reply-To: Charles P. Daghlian's message <5966918@donner.Dartmouth.EDU> of 13 July 1993 References: <5966918@donner.Dartmouth.EDU> From: "John Ladwig" To: Subject: Re: undeliverable mail messages Date: Tue, 13 Jul 93 14:02:12 CDT Charles P. Daghlian writes on 13 July 1993 at 09:20:19 -0500 > Would you please change your mail system so we stop getting the many > undeliverable mail messages. If this is not possible, please remove me from > the list. I'm looking into it... From jladwig@soils.umn.edu Tue Jul 13 09:02:55 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA15971; Tue, 13 Jul 1993 13:59:48 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA08321; Tue, 13 Jul 93 14:02:55 CDT Message-Id: <9307131902.AA08321@saturn.soils.umn.edu> In-Reply-To: Doug Morris's message <199307131515.AA01085@bmrl.med.uiuc.edu> of 13 July 1993 References: <199307131515.AA01085@bmrl.med.uiuc.edu> From: "John Ladwig" To: Subject: Re: Undeliverable Mail Date: Tue, 13 Jul 93 14:02:55 CDT Doug Morris writes on 13 July 1993 at 10:12:01 -0500 > Please put a filter on listserv to avoid massed amounts of > undeliverable mail. ... am working on it ... From Sylwester_Chyb@agcs.psu.edu Tue Jul 13 15:57:35 1993 Received: from agcs.psu.edu (agcs.cas.psu.edu) by nx1.soils.umn.edu (5.65c) id AA17019; Tue, 13 Jul 1993 15:57:35 -0500 Return-Path: Message-Id: <199307132057.AA17019@nx1.soils.umn.edu> Return-Receipt-To: "Sylwester Chyb" From: "Sylwester Chyb" To: nih-image@soils.umn.edu Subject: video rate acquisition problem Date: 13 Jul 1993 17:04:13 U I am using Image 1.50b95 on Quadra 800 (24 MB RAM) with Scion LG-3 frame grabber (16 MB expanded memory). I can average frames at video rate but when making movie the highest rate I get is about 15 frames per second. I would be grateful for any help from more experienced users. Thanks, Sylwester Chyb Penn State University From wayne@zippy.nimh.nih.gov Tue Jul 13 16:04:19 1993 Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA17084; Tue, 13 Jul 1993 16:02:55 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA13968; Tue, 13 Jul 93 16:18:55 -0400 Message-Id: <9307132018.AA13968@zippy.nimh.nih.gov> Date: Tue, 13 Jul 1993 16:04:19 +0000 To: nih-image@soils.umn.edu From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: crashes on image 1.49 >I am having continuous crashes with the program Image 1.49 that >happen even when I am not touching the computer keyboard or >mouse. My technician reports the same problem. I appears to occur >when I am at the microscope making adjustments in the light and/or >focus. Perhaps a saturation occurs just before the crash. >Occasionally crashes the entire system but more often is the Image >application that quits with an error of the type 1. I am using Image >on a Quadra 950 with Scion LG-3. The version 1.47 works >flawlessly. Do both versions of the program have the same amount of memory allocated to them? Have you tried using a fresh copy of 1.49? >I have been reading about Image 1.50b and others. Does the b >stand for beta?, if so, when could we expect a bug free Image >1.50? is it "risky" to use 1.50b? should I wait to download the final >version? The "b" stands for beta. There will never be a version of Image that is entirely free of bugs; all complex programs have bugs. The 1.50 beta on Zippy should have fewer bugs than 1.49 since it fixes at least seven bugs that were in 1.49. Of course, there is always the possibility that it introduces some new bugs, but usually I am able to stomp out bugs faster than I create them --wayne From dmorris@bmrl.med.uiuc.edu Tue Jul 13 12:41:23 1993 Received: from bmrl.med.uiuc.edu by nx1.soils.umn.edu (5.65c) id AA17689; Tue, 13 Jul 1993 17:36:56 -0500 Return-Path: Received: by bmrl.med.uiuc.edu id AA05455 (5.67a/IDA-1.4.2 for nih-image@soils.umn.edu); Tue, 13 Jul 1993 17:41:23 -0500 From: Doug Morris Message-Id: <199307132241.AA05455@bmrl.med.uiuc.edu> Subject: Re: Undeliverable Mail To: nih-image@soils.umn.edu Date: Tue, 13 Jul 1993 17:41:23 -0500 (CDT) In-Reply-To: <9307131902.AA08321@saturn.soils.umn.edu> from "John Ladwig" at Jul 13, 93 02:46:12 pm X-Mailer: ELM [version 2.4 PL21] Content-Type: text Content-Length: 222 Thanks. > > Doug Morris writes on 13 July 1993 at 10:12:01 -0500 > > > Please put a filter on listserv to avoid massed amounts of > > undeliverable mail. > > .. am working on it ... > > From pwf@unet.umn.edu Tue Jul 13 12:42:40 1993 Received: from unet.unet.umn.edu by nx1.soils.umn.edu (5.65c) id AA17722; Tue, 13 Jul 1993 17:38:14 -0500 Return-Path: Received: by unet.unet.umn.edu (5.65c) id AA03772; Tue, 13 Jul 1993 17:42:40 -0500 Message-Id: <199307132242.AA03772@unet.unet.umn.edu> In-Reply-To: <9307131902.AA08321@saturn.soils.umn.edu> from "John Ladwig" at Jul 13, 93 02:49:06 pm X-Mailer: ELM [version 2.4 PL21] Mime-Version: 1.0 Content-Length: 2211 From: "Paul W. Fakler" To: nih-image@soils.umn.edu Cc: pwf@unet.unet.umn.edu (Paul W. Fakler) Subject: Re: mailing list setup Date: Tue, 13 Jul 1993 17:42:40 -0500 (CDT) Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit > From nih-image@soils.umn.edu Tue Jul 13 17:07:26 1993 > From: "John Ladwig" > To: Multiple recipients of list > Subject: Re: Undeliverable Mail > > Doug Morris writes on 13 July 1993 at 10:12:01 -0500 > > > Please put a filter on listserv to avoid massed amounts of > > undeliverable mail. > > .. am working on it ... The problem is that mail is going out with RFC821 SMTP command of: Mail From: When you want it to be Mail From: You can get this by doing the following: Set up your mailing list in /etc/aliases as follows: net-people: "|/usr/local/sbin/sendmail -odb -oi -om -os -fowner-net-people@mail.unet.umn.edu net-people-list" net-people-list: :include:/etc/al/net-people owner-net-people: unet net-people-request: unet Substituting for your list and locations, you get: nih-image: "|/usr/lib/sendmail -odb -oi -om -os -fowner-nih-image@mail.unet.umn.edu nih-image-list" nih-image-list: :include:/etc/nih-image owner-nih-image: jladwig nih-image-request: jladwig and create a link between /etc/nih-image and where you have the mailing list. run newaliases, etc... Rejected mail will now go to jladwig, or whereever (one copy), and not to the whole list. jladwig can then get a look at problem deliveries without affecting the whole list. I also have made mods to sendmail 5.65c to support large mailing lists. I'll talk to you about them if you want. Paul. *-----------------------------------------------------------------------* | Paul W. Fakler | *---------------------------------*-------------------------------------* | Internet : pwf@unet.umn.edu | Networking Services | | Bitnet : pwf@umnacvx.bitnet | Computer and Information Services | | Desk : +1 612 626 1654 | University of Minnesota | | Problems : +1 612 625 0006 | 130 Lind Hall, 207 Church St SE | | FAX : +1 612 626 1002 | Minneapolis MN 55455-0134, USA | *---------------------------------*-------------------------------------* From jladwig@soils.umn.edu Tue Jul 13 15:14:38 1993 Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA18506; Tue, 13 Jul 1993 20:11:31 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA09145; Tue, 13 Jul 93 20:14:38 CDT Date: Tue, 13 Jul 93 20:14:38 CDT From: "John Ladwig" Message-Id: <9307140114.AA09145@saturn.soils.umn.edu> To: Subject: Re: mailing list setup In-Reply-To: "Paul W. Fakler"'s message <199307132242.AA03772@unet.unet.umn.edu> of 13 July 1993 References: <199307132242.AA03772@unet.unet.umn.edu> "Paul W. Fakler" writes on 13 July 1993 at 17:41:20 -0500 > The problem is that mail is going out with RFC821 SMTP command of: > > Mail From: > > When you want it to be > > Mail From: > > > You can get this by doing the following: > > Set up your mailing list in /etc/aliases as follows: > > net-people: "|/usr/local/sbin/sendmail -odb -oi -om -os -fowner-net-people@mail.unet.umn.edu net-people-list" > net-people-list: :include:/etc/al/net-people > owner-net-people: unet > net-people-request: unet Umm, except that my aliases file looks like: # Listserver section # listserv: "|/usr/server/catmail -r -f" nih-image: "|/usr/server/catmail -L NIH-IMAGE -f" nih-image-request: owner-nih-image listserv-manager: jladwig owner-nih-image: jladwig as I am running unix-listserv 5.5 (from tasos@cs.bu.edu). I am investigating a newer version of unix-listserver (6.0 with 6.0a to be released shortly), as Tasos (the author) says it will have somewhat better error and loop recognition. Also, sendmail never sees any outgoing message; they're delivered directly by a listserver daemon which handles digesting, archiving, and other useful things. I may be able to get a temporary fix into my nih-image configuration, however, at least as regards the From: processing. Ninety nine and some fraction percent of all bounce messages go (correctly) back along the Errors-To: specification, but I'll see if that hack makes things work OK. When that fails, I have set my list to reject messages from unsubscribed parties, which gets some more of the unobservant mailers. From nih-image@soils.umn.edu Tue Jul 13 17:47:26 1993 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA17859; Tue, 13 Jul 1993 17:47:26 -0500 Return-Path: Message-Id: <01H0I3QPBIZMAFTVLG@vx.cis.umn.edu> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas Status: U From: nih-image@soils.umn.edu Sender: nih-image@soils.umn.edu To: postman@frmop22.cnusc.fr Cc: owner-nih-image@soils.umn.edu Subject: Undelivered mail Date: Tue, 13 Jul 1993 17:47:26 -0500 postman@frmop22.cnusc.fr: You are not subscribed to nih-image@soils.umn.edu. Your message is returned to you unprocessed. If you want to subscribe, send mail to listserv@soils.umn.edu with the following request: subscribe NIH-IMAGE Your Name ------------------------------------------------------------------------------- Your mail was not delivered to some or all of its intended recipients for the following reason(s): 6001 no valid recipients for this node [ ... ] From mvivino@helix.nih.gov Wed Jul 14 04:19:36 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["616" "Wed" "14" "July" "93" "08:19:36" "-0400" "mvivino@helix.nih.gov" "mvivino@helix.nih.gov" nil "21" "Re: Application: DNA gels" nil nil nil "7"]) Received: from helix.nih.gov by nx1.soils.umn.edu (5.65c) id AA21336; Wed, 14 Jul 1993 07:15:12 -0500 Return-Path: Received: from mavmac.dcrt.nih.gov by helix.nih.gov (5.64/1.35(helix-1.0)) id AA19678; Wed, 14 Jul 93 08:19:36 -0400 Date: Wed, 14 Jul 93 08:19:36 -0400 Message-Id: <9307141219.AA19678@helix.nih.gov> To: From: mvivino@helix.nih.gov X-Sender: mvivino@128.231.128.13 Subject: Re: Application: DNA gels >1) I need a UV filter for the camera that will filter the light from > the UV light box used to visualize the DNA. Most standard > photographic filters are not adequate. Any suggestions will > be appriciated. Try this company, they have a full line of high grade filters. The filters tend to attenuate light out of the passband better than any that I have seen. I don't have any UV filters from them, but have found some excellent ones for Coomassie blue and IR surpressing. Corian Corp 1-508-429-5065 73 Jeffrey Ave. Holliston, MA 01746-208 Mark Vivino NIH/DCRT mvivino@helix.nih.gov From wayne@zippy.nimh.nih.gov Wed Jul 14 10:16:56 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["518" "Wed" "14" "July" "1993" "10:16:56" "+0000" "Wayne Rasband" "wayne@zippy.nimh.nih.gov" nil "11" "Re: video rate acquisition problem" nil nil nil "7"]) Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA22316; Wed, 14 Jul 1993 10:14:44 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA15733; Wed, 14 Jul 93 10:31:30 -0400 Message-Id: <9307141431.AA15733@zippy.nimh.nih.gov> Date: Wed, 14 Jul 1993 10:16:56 +0000 To: From: wayne@zippy.nimh.nih.gov(Wayne Rasband) Subject: Re: video rate acquisition problem >I am using Image 1.50b95 on Quadra 800 (24 MB RAM) with Scion LG-3 frame >grabber (16 MB expanded memory). I can average frames at video rate but when >making movie the highest rate I get is about 15 frames per second. I would be >grateful for any help from more experienced users. The Make Movie command does not yet support video rate capture using the LG-3's expanded memory. You might want to give Tod Weinberg of Scion a call. I believe he has a modified version of Image that does video rate capture. --wayne From dave@nucleus.immunol.washington.edu Wed Jul 14 02:13:56 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["1439" "Wed" "14" "July" "93" "09:13:56" "-0700" "Dave Coder" "dave@nucleus.immunol.washington.edu" nil "37" "Re: Application: DNA gels" nil nil nil "7"]) Received: from nucleus.immunol.washington.edu by nx1.soils.umn.edu (5.65c) id AA22692; Wed, 14 Jul 1993 11:09:46 -0500 Return-Path: Received: by nucleus.immunol.washington.edu (NX5.67c/NX3.0M) id AA20283; Wed, 14 Jul 93 09:13:56 -0700 Date: Wed, 14 Jul 93 09:13:56 -0700 From: Dave Coder Message-Id: <9307141613.AA20283@nucleus.immunol.washington.edu> Received: by NeXT.Mailer (1.87.1.RR) Received: by NeXT Mailer (1.87.1.RR) To: Subject: Re: Application: DNA gels >>1) I need a UV filter for the camera that will filter the light from >> the UV light box used to visualize the DNA. Most standard >> photographic filters are not adequate. Any suggestions will >> be appriciated. If filtering out uv to visualize ethidium fluorescence is the goal, then there should be lots of photographic filters that should do the job. UV is generally easy to filter out. I would think that uv/haze filters should do the job, or to be really sure, a yellow long pass filter (50% transmission peak at 550nm) will easily do the job. Color correction filters (those for correcting the response of color films to different color temperature light sources) such as 85 or 81A would probably work as well since they absorb at the blue end of the spectrum. Filter fluorescence for this application should be undetectable; it's easy to check. One of several advantages of using photographic filters with standard camera lenses is that you can get the filters in diameters to screw into the camera lens. If you want to use a variety of filters available only as squares, then there are mounts that make it very easy to slip in various filters. >Try this company, they have a full line of high grade filters. >Corian Corp >1-508-429-5065 >73 Jeffrey Ave. >Holliston, MA 01746-208 Corian makes lots of filters for many applications. Dave Coder dcoder@u.washington.edu From @dka.smhms.imperial.ac.uk:rph30@uk.ac.ic.sm.mailhost Wed Jul 14 11:36:37 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["6261" "Wed" "14" "July" "1993" "17:36:37" "CST" "Richard P Harbottle" "r.harbottle@smhms.imperial.ac.uk" nil "165" "Re: Application: DNA gels" nil nil nil "7"]) Received: from sun2.nsfnet-relay.ac.uk by nx1.soils.umn.edu (5.65c) id AA22888; Wed, 14 Jul 1993 11:38:22 -0500 Return-Path: <@dka.smhms.imperial.ac.uk:rph30@uk.ac.ic.sm.mailhost> Via: uk.ac.imperial.smhms.dka; Wed, 14 Jul 1993 17:42:27 +0100 X-Nupop-Charset: English Date: Wed, 14 Jul 1993 17:36:37 CST From: Richard P Harbottle Sender: rph30@uk.ac.ic.sm.mailhost Reply-To: r.harbottle@smhms.imperial.ac.uk Message-Id: <63399.r.harbottle@sm.ic.ac.uk> To: nih-image@soils.umn.edu Subject: Re: Application: DNA gels In Message Wed, 14 Jul 1993 11:17:49 -0500, Dave Coder writes: > >>>1) I need a UV filter for the camera that will filter the light >from >>> the UV light box used to visualize the DNA. Most standard >>> photographic filters are not adequate. Any suggestions >will >>> be appriciated. > >If filtering out uv to visualize ethidium fluorescence is the goal, >then there should be lots of photographic filters that should do the >job. UV is generally easy to filter out. I would think that uv/haze >filters should do the job, or to be really sure, a yellow long pass >filter (50% transmission peak at 550nm) will easily do the job. Color >correction filters (those for correcting the response of color films >to different color temperature light sources) such as 85 or 81A would >probably work as well since they absorb at the blue end of the >spectrum. Filter fluorescence for this application should be >undetectable; it's easy to check. One of several advantages of using >photographic filters with standard camera lenses is that you can get >the filters in diameters to screw into the camera lens. If you want >to use a variety of filters available only as squares, then there are >mounts that make it very easy to slip in various filters. > > >>Try this company, they have a full line of high grade filters. > >>Corian Corp >>1-508-429-5065 >>73 Jeffrey Ave. >>Holliston, MA 01746-208 > >Corian makes lots of filters for many applications. > >Dave Coder >dcoder@u.washington.edu To photograph Ethidium fluorescence on our Polaroid camera we use a Tiffen Ser.6 15 Orange (U.S.A.) filter. The UV transluminator manufacturer recommends using a Kodak Wratten No. 23A filter. This blocks UV and the visible blue light from the transluminator. I hope this helps. rph30@sm.ic.ac.uk ******* Richard ******* From chien@jeeves.UCSD.EDU Wed Jul 14 06:37:53 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["73" "Wed" "14" "July" "93" "13:37:53" "PDT" "Chi-Bin Chien" "chien@jeeves.UCSD.EDU " nil "1" "macro control of Scion gain and brightness" nil nil nil "7"]) Received: from ucsd.edu by nx1.soils.umn.edu (5.65c) id AA24252; Wed, 14 Jul 1993 15:33:30 -0500 Return-Path: Received: from jeeves.ucsd.edu by ucsd.edu; id AA21115 sendmail 5.67/UCSD-2.2-sun via SMTP Wed, 14 Jul 93 13:37:55 -0700 for nih-image@soils.umn.edu Received: by jeeves.UCSD.EDU (4.1/UCSDGENERIC.4) id AA20436 to nih-image@soils.umn.edu; Wed, 14 Jul 93 13:37:53 PDT Date: Wed, 14 Jul 93 13:37:53 PDT From: chien@jeeves.UCSD.EDU (Chi-Bin Chien) Message-Id: <9307142037.AA20436@jeeves.UCSD.EDU> To: benedict@jeeves.ucsd.edu, nih-image@soils.umn.edu Subject: macro control of Scion gain and brightness Is there a way to set the gain and brightness controls of the Scion LG-3 From a macro? I had assumed that there was, but after much flipping through Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["36" "" "" "" "" "" "" "a" "a" nil "2" "" nil nil nil ""]) the Change History and manual, I have been unable to find these functions, which would be useful I think. Chi-Bin Chien chien@jeeves.ucsd.edu From sarmienu@rnisd0.DNET.roche.com Thu Jul 15 05:52:48 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["804" "Thu" "15" "July" "93" "09:52:48" "EDT" "sarmienu@rnisd0.DNET.roche.com" "sarmienu@rnisd0.DNET.roche.com" nil "26" "getting NIH.image 1.50b97" nil nil nil "7"]) Received: from GATEKEEPER.ROCHE.COM by nx1.soils.umn.edu (5.65c) id AA28438; Thu, 15 Jul 1993 08:48:28 -0500 Return-Path: Received: by gatekeeper.roche.com (5.65/fma-120691); id AA12962; Thu, 15 Jul 93 09:52:49 -0400 Received: by mailgate.roche.com (5.65/fma-120691); id AA11468; Thu, 15 Jul 93 09:51:21 -0400 From: sarmienu@rnisd0.DNET.roche.com Message-Id: <9307151351.AA11468@mailgate.roche.com> Received: from rnisd0.enet; by inet.enet; Thu, 15 Jul 93 09:52:48 EDT Date: Thu, 15 Jul 93 09:52:48 EDT To: nih-image@soils.umn.edu Apparently-To: nih-image@soils.umn.edu Subject: getting NIH.image 1.50b97 Wayne: I got the following message when trying to retrieve nih-image 1.50b97: --- connecting to zippy.nimh.nih.gov... Connecting to zippy.nimh.nih.gov 220 zippy FTP server (Version 1.7.109.2 Tue Jul 28 23:32:34 GMT 1992) ready. --- logging in as user=anonymous password=-ftpmail/Sarmienu@RNISD0.DNET.ROCHE.C* ---> USER anonymous 331 Guest login ok, send ident as password. ---> PASS 230 Guest login ok, access restrictions apply. ---> TYPE A 200 Type set to A. === getting '/pub/nih-image150b97.hqx'... ---> PORT 16,1,0,6,18,76 200 PORT command successful. ---> RETR /pub/nih-image150b97.hqx 550 /pub/nih-image150b97.hqx: No such file or directory. Failure on RETR command !!! cannot initiate RETR ---> (end of ftpmail session) What am I doing wrong?? Did you uploaded a new version? From afruppe@pxdvgx.erenj.com Thu Jul 15 11:24:50 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["1206" "Thu" "15" "July" "1993" "16:24:50" "-0500" "Albert Ruppert" "afruppe@pxdvgx.erenj.com " nil "37" "Re: getting NIH.image 1.50b97" nil nil nil "7"]) Received: from erenj.com (ereapp.erenj.com) by nx1.soils.umn.edu (5.65c) id AA00535; Thu, 15 Jul 1993 15:17:26 -0500 Return-Path: Received: by erenj.com (5.65/mjr-920806); id AA23590; Thu, 15 Jul 93 16:21:47 -0400 Received: by eredns.erenj.com (5.65/bdb-mailv1.3-erenj); id AA19667; Thu, 15 Jul 93 16:21:39 -0400 Posted-Date: Thu, 15 Jul 1993 16:24:50 -0500 Message-Id: <9307152021.AA19667@eredns.erenj.com> Date: Thu, 15 Jul 1993 16:24:50 -0500 To: From: afruppe@pxdvgx.erenj.com (Albert Ruppert) Subject: Re: getting NIH.image 1.50b97 >Wayne: > >I got the following message when trying to retrieve nih-image 1.50b97: > >--- connecting to zippy.nimh.nih.gov... >Connecting to zippy.nimh.nih.gov >220 zippy FTP server (Version 1.7.109.2 Tue Jul 28 23:32:34 GMT 1992) ready. >--- logging in as user=anonymous >password=-ftpmail/Sarmienu@RNISD0.DNET.ROCHE.C* >---> USER anonymous >331 Guest login ok, send ident as password. >---> PASS >230 Guest login ok, access restrictions apply. >---> TYPE A >200 Type set to A. >=== getting '/pub/nih-image150b97.hqx'... >---> PORT 16,1,0,6,18,76 >200 PORT command successful. >---> RETR /pub/nih-image150b97.hqx >550 /pub/nih-image150b97.hqx: No such file or directory. >Failure on RETR command >!!! cannot initiate RETR >---> (end of ftpmail session) > >What am I doing wrong?? > >Did you uploaded a new version? You don't have the full path in your get commannd. Try get /pub/nih-image/nih-image150b97.hqx =========================================================================== All thoughts expressed are mine and are not intended to reflect the opinions of my employer. (disclaimer) Al Ruppert afruppe@erenj.com (908) 730-2979 From jladwig@soils.umn.edu Fri Jul 16 03:06:33 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["4139" "Fri" "16" "July" "93" "08:06:33" "CDT" "\"John Ladwig\"" "jladwig@soils.umn.edu" nil "105" "Looping messages " "^From:" nil nil "7"]) Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA04889; Fri, 16 Jul 1993 08:03:30 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA17454; Fri, 16 Jul 93 08:06:33 CDT Message-Id: <9307161306.AA17454@saturn.soils.umn.edu> From: "John Ladwig" To: nih-image@soils.umn.edu Subject: Looping messages Date: Fri, 16 Jul 93 08:06:33 CDT Yeah, I see 'em too. I've appealed to the administrator of the host generating these messages, and am exploring internal filter possibilities (don't know why the one I tried didn't work...) Please don't panic, and please don't send copies back to the list asking what this is all about... Thanks bunches.. -jml From jladwig Fri Jul 16 03:38:54 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["231" "Fri" "16" "July" "1993" "08:38:54" "-0500" "\"John Ladwig\"" "jladwig" nil "6" "... just testing ..." nil nil nil "7"]) Received: by nx1.soils.umn.edu (5.65c) id AA05347; Fri, 16 Jul 1993 08:38:56 -0500 Return-Path: From: "John Ladwig" Message-Id: <199307161338.AA05347@nx1.soils.umn.edu> Subject: ... just testing ... To: nih-image Date: Fri, 16 Jul 1993 08:38:54 -0500 (CDT) X-Mailer: ELM [version 2.4 PL22] Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Content-Length: 231 I'm still trying to clamp down on the loop; I just need to know if I've clamped down too tight. -- UMN Department of Soil Science; St. Paul, MN Internet: john.ladwig@soils.umn.edu Fidonet: John Ladwig 1:282/341 From steven@cell.BIH.Harvard.Edu Fri Jul 16 04:21:31 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["2333" "Fri" "16" "July" "93" "08:21:31" "EDT" "Steven Blechner" "steven@cell.BIH.Harvard.Edu " nil "59" "SelectPic('Title') Advice Needed..." "^From:" nil nil "7"]) Received: from cell.bih.harvard.edu by nx1.soils.umn.edu (5.65c) id AA06971; Fri, 16 Jul 1993 10:39:56 -0500 Return-Path: Received: by cell.bih.harvard.edu (4.1/SMI-DDN) id AA03427; Fri, 16 Jul 93 08:21:31 EDT Message-Id: <9307161221.AA03427@cell.bih.harvard.edu> From: steven@cell.BIH.Harvard.Edu (Steven Blechner) To: nih-image@soils.umn.edu Subject: SelectPic('Title') Advice Needed... Date: Fri, 16 Jul 93 08:21:31 EDT We are using Image version 1.49. We cannot currently upgrade to the newest version easily, but our lives would be infinitely simpler if we could add the new SelectPic by 'Titile' options to our macros. Can anyone assist me in adding that new functionality to the older version ? I am moderately Pascal proficient. Thank you. Sincerely, steve Steven L. Blechner, Ph.D. Director, Molecular Computing Facility Beth Israel Hospital Boston, MA E-mail: steven@cell.bih.harvard.edu From norm_hurst@maca.sarnoff.com Fri Jul 16 10:43:11 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["1274" "" "16" "July" "1993" "11:40:42" "U" "\"Norm Hurst\"" "norm_hurst@maca.sarnoff.com" nil "38" "Coordinated Pan and Zoom" nil nil nil "7"]) Received: from nova (nova.sarnoff.com) by nx1.soils.umn.edu (5.65c) id AA07027; Fri, 16 Jul 1993 10:43:11 -0500 Return-Path: Received: from maca.sarnoff.com by nova (4.1/SMI-4.0) id AA24786; Fri, 16 Jul 93 11:47:20 EDT Message-Id: <9307161547.AA24786@nova> From: "Norm Hurst" To: "NIH Image user group" Subject: Coordinated Pan and Zoom Date: 16 Jul 1993 11:40:42 U Coordinated Pan and Zoom 7/16/93 10:36 AM Wayne- Is there a mode for Image that allows me to zoom multiple windows simultaneously and then pan (with the hand tool) several images simulataneously? We use Image to compare the results of different processing algorithms, and this would allow us to roam over several images at once. By the way... I have tried to use the macro language for signal processing, but the automatic scaling of things like subtract and convolve messes me up. I wish I had a few function calls like these: A function for precision gain and offset y = (x + offset1)*gain + offset2 A function for adding two images y = x1 + x2 + offset A function for multiplying two images y = (x1 + offset1)*(x2 + offset2) + offset3 A function for convolution that doesn't decide what the offset and tap scale factor should be: y = conv(x, 'taps_filename') + offset I've tried to implement certain signal processing algorithms (like an NTSC-to-RGB decoder), but I can't seem to get around the automatic scale and offset properties of subtract, multiply and convolve. Am I missing something? Norm Hurst (Mr. MPEG) David Sarnoff Reseach Center From jamiel@sybase.com Tue Mar 16 05:13:16 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["873" "Tue" "16" "March" "1993" "13:13:16" "-0800" "Jamie Lawrence" "jamiel@sybase.com " nil "18" "" "^From:" nil nil "3"]) Received: from halon.sybase.com by nx1.soils.umn.edu (5.65c) id AA09043; Fri, 16 Jul 1993 15:07:12 -0500 Return-Path: Received: from sybase.com (sybgate.sybase.com) by halon.sybase.com (5.0/SMI-SVR4/SybFW4.0) id AA06010; Fri, 16 Jul 93 13:12:32 PDT Received: from ralph.sybgate.sybase.com by sybase.com (4.1/SMI-4.1/SybH3.2) id AA12806; Fri, 16 Jul 93 13:12:23 PDT Received: from [130.214.134.15] by ralph.sybgate.sybase.com (4.1/SMI-4.1/SybEC3.1) id AA27897; Fri, 16 Jul 93 13:12:18 PDT Message-Id: <9307162012.AA27897@ralph.sybgate.sybase.com> X-Sender: jamiel@ralph.sybase.com Content-Length: 874 From: jamiel@sybase.com (Jamie Lawrence) To: Date: Tue, 16 Mar 1993 13:13:16 -0800 Looks like the amazing rubber mail from earlier has been solved :) 1)Does anyone know what a good reference for determining how a given convolve filter will effect an image? Also the other way around- how to determine the proper array to achive a specific effect. Here I am talking about effects more suited artistic type stuff than image analysis. 2) as is probably clear from the above question, I don't really understand how these things work, but is there any good way to convert a picture from polar to retangular mapping, also reverse, a la photoshop? I have some (tinsy) experience with the macro language, so that is fine. I am kind of guessing that this is not possible, due to what I imagine is involved, but never hurts to ask (of to ramble on nonstop like this- sorry, not enough sleep lately :)) Any help greatly apprecieated... jamie jamiel@sybase.com From wayne@zippy.nimh.nih.gov Fri Jul 16 16:20:17 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["1289" "Fri" "16" "July" "1993" "16:20:17" "+0000" "Wayne Rasband" "wayne@zippy.nimh.nih.gov" nil "28" "Re: Coordinated Pan and Zoom" "^From:" nil nil "7"]) Received: from zippy.nimh.nih.gov by nx1.soils.umn.edu (5.65c) id AA09692; Fri, 16 Jul 1993 16:18:08 -0500 Return-Path: Received: from pico.nimh.nih.gov by zippy.nimh.nih.gov (1.37.109.4/zippy-1.0) id AA22532; Fri, 16 Jul 93 16:35:03 -0400 Message-Id: <9307162035.AA22532@zippy.nimh.nih.gov> From: wayne@zippy.nimh.nih.gov(Wayne Rasband) To: Subject: Re: Coordinated Pan and Zoom Date: Fri, 16 Jul 1993 16:20:17 +0000 >Is there a mode for Image that allows me to zoom multiple windows >simultaneously and then pan (with the hand tool) several images >simulataneously? We use Image to compare the results of different processing >algorithms, and this would allow us to roam over several images at once. There is a poorly documented synchronized scrolling feature in Image. To try it out, open two or more windows(they must be the same size), tile them using Tile Windows, zoom in on one of them, then pan one of the images with the option key down. Notice how all the other images zoom and pan to the same relative location. >I have tried to use the macro language for signal processing, but the automatic >scaling of things like subtract and convolve messes me up. I am considering adding a macro function(and corresponding menu command) something like the following: ImageArithmetic('op',' pic1', 'pic2', gain, offset) 'op'('Add', 'Sub', 'Mult', etc.) is the operation to be performed, 'pic1' and 'pic2' are the two images to be operated on. Pixel values resulting From the operation would by multiplied by the gain and then the offset Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["142" "" "" "" "" "" "" "the" "the" nil "5" "" nil nil nil ""]) would be added. For convolution, I'm thinking of allowing keywords to be added to the first line of the kernel file to control how scaling is done. --wayne From corbit@esseX.stfx.ca Fri Jul 16 16:20:41 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["2203" "Fri" "16" "July" "1993" "18:24:25" "AST" "John Corbit" "corbit@esseX.stfx.ca" nil "54" "40 undeliv.mail.messages today!!! please stop." "^From:" nil nil "7"]) Received: from esseX.StFX.CA by nx1.soils.umn.edu (5.65c) id AA09785; Fri, 16 Jul 1993 16:20:41 -0500 Return-Path: Received: by esseX.stfx.ca (MX V2.3) id 7103; Fri, 16 Jul 1993 18:24:25 AST Message-Id: <0096F98E.E6E77880.7103@esseX.stfx.ca> From: John Corbit To: nih-image@soils.umn.edu Subject: 40 undeliv.mail.messages today!!! please stop. Date: Fri, 16 Jul 1993 18:24:25 AST From: MX%"nih-image@soils.umn.edu" 16-JUL-1993 17:33:34.90 To: CORBIT CC: Subj: Undeliverable Mail Return-Path: <@esseX.stfx.ca:nih-image@soils.umn.edu> Received: from mail.tc.umn.edu by esseX.stfx.ca (MX V2.3) with SMTP; Fri, 16 Jul 1993 17:33:01 AST Received: from nx1.soils.umn.edu by mail.tc.umn.edu id SMTP-0012c470d11006990; Fri, 16 Jul 93 15:18:26 -0500 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA09154; Fri, 16 Jul 1993 15:13:48 -0500 Date: Fri, 16 Jul 1993 15:13:48 -0500 Message-ID: <199307162007.AA09064@nx1.soils.umn.edu> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: To: Multiple recipients of list Subject: Undeliverable Mail Attempting to deliver following mail to recipient(s): cc1.kuleuven.ac.be unable to connect for 3 days to recipient host. Delivery will be attempted for a total of 17 days. ** Text of Mail follows ** Received: from nx1.soils.umn.edu by cc1.kuleuven.ac.be (IBM VM SMTP V2R2) with TCP; Tue, 13 Jul 93 21:47:23 +0200 Received: from (localhost) by nx1.soils.umn.edu (5.65c) id AA16346; Tue, 13 Jul 1993 14:42:16 -0500 Date: Tue, 13 Jul 1993 14:42:16 -0500 Message-Id: <9307131902.AA08318@saturn.soils.umn.edu> Comment: NIH Image Distribution List Originator: nih-image@soils.umn.edu Errors-To: owner-nih-image@soils.umn.edu Reply-To: Sender: nih-image@soils.umn.edu Version: 5.5 -- Copyright (c) 1991/92, Anastasios Kotsikonas From: "John Ladwig" To: Multiple recipients of list Subject: Re: undeliverable mail messages Charles P. Daghlian writes on 13 July 1993 at 09:20:19 -0500 > Would you please change your mail system so we stop getting the many > undeliverable mail messages. If this is not possible, please remove me from > the list. I'm looking into it... From jladwig@soils.umn.edu Fri Jul 16 12:34:12 1993 Status: O X-VM-v5-Data: ([nil t nil nil nil nil nil nil nil] ["615" "Fri" "16" "July" "93" "17:34:12" "CDT" "\"John Ladwig\"" "jladwig@soils.umn.edu" nil "17" "Re: " "^From:" nil nil "7"]) Received: from saturn.soils.umn.edu by nx1.soils.umn.edu (5.65c) id AA10301; Fri, 16 Jul 1993 17:31:11 -0500 Return-Path: Received: by saturn.soils.umn.edu (4.1) id AA19171; Fri, 16 Jul 93 17:34:12 CDT Message-Id: <9307162234.AA19171@saturn.soils.umn.edu> In-Reply-To: Jamie Lawrence's message <9307162012.AA27897@ralph.sybgate.sybase.com> of 16 July 1993 References: <9307162012.AA27897@ralph.sybgate.sybase.com> From: "John Ladwig" To: Subject: Re: Date: Fri, 16 Jul 93 17:34:12 CDT Jamie Lawrence writes on 16 July 1993 at 15:07:26 -0500 > Looks like the amazing rubber mail from earlier has been solved :) I'm afraid not - *please* bear with me on this - I'm at the mercy of some faceless administrator somewhere in Belgium on this one. I've stopped the flow of new messages into the bounce stream, but I can't seem to do anything automated about the recycling messages "will keep trying for 17 days ack!). If it *really* bothers evryone, I could shut down the list for two weeks or until I get a response from the postmaster of the offending system. Comments? -jml