Curriculum Vitae

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JENNIFER R. ANTHONY jranthony@berkeley.edu Business Address: Home Address: University of California, Berkeley 16120 Silverleaf Drive 717 Potter Street, Room 263B, MC 3224 San Lorenzo, CA 94580 Berkeley, CA 94720-3224 (510) 317-0854 (510) 495-2627 (608) 332-9430 cell ____________________________________________________________________________________________

EDUCATION: University of California-Berkeley, Berkeley, CA Postdoctoral Fellow Advisor: Dr. Jay Keasling, Professor of Chemical Engineering

University of Wisconsin-Madison, Madison, WI Ph.D. in Microbiology, April 2005 Advisor: Dr. Timothy Donohue, Professor of Bacteriology

University of Wisconsin-LaCrosse, College of Arts and Sciences, LaCrosse, WI Bachelor of Science-Microbiology, May 1998 University of Wisconsin La-Crosse Dean's List

AWARDS: - Ruth L. Kirschstein National Research Service Award. 2005-present. National Institute of Health-Department of Health and Human Services. - Thomsen Wisconsin Distinguished Graduate Fellowship. 2004-2005. College of Agricultural and Life Sciences, University of Wisconsin-Madison. - Jerome J. Stefaniak Predoctoral Fellowship. 2002-2003. Department of Bacteriology, University of Wisconsin-Madison.

RESEARCH Postdoctoral Fellow EXPERIENCE: University of California-Berkeley May 2005-present Engineering Terpene Synthases for Improved Function in Escherichia coli - Employed mutagenesis and directed evolution studies on the terpene synthase, amorpha- 4,11-diene synthase to improve production of the anti-malarial precursor, amorpha-4,11- diene. - Constructed a modular vector for expression of a heterologous amorpha-4,11-diene pathway in E. coli that resulted in a 3-fold increase in production. - Currently have 2 manuscripts in preparation

Graduate Fellow University of Wisconsin-Madison January 2000-April 2005 Identification of a physiological role for the Rhodobacter sphaeroides sigma factor, RpoE. - Determined singlet oxygen stress initiated a RpoE-dependent transcriptional response by monitoring cell growth and RpoE activity in response to various environmental stresses. - Purified RNA from R. sphaeroides and employed DNA microarray technology to ascertain members of the RpoE regulon. Results were confirmed in vivo using reporter gene assays, and in vitro via transcription assays. - Examined the protein-protein interactions between RpoE and its anti-sigma factor, ChrR. Performed random mutagenesis of RpoE to identify residues important for complex formation with ChrR. Characterized various RpoE mutants in vivo, and purified proteins for in vitro analysis that included HPLC and LRET assays.

	- Authored or co-authored 5 papers in peer-reviewed journals.

Jennifer R. Anthony

Research Technician University of Wisconsin-Madison June 1998-September 1999 - Studied in vitro and in vivo activation of the anaerobically-induced promoter, cycAP2, by the photosynthetic response regulator PrrA of Rhodobacter sphaeroides.

Undergraduate Research Assistant University of Wisconsin-LaCrosse September 1996-May 1998 - Undergraduate research that examined the role of PQQ biosynthetic genes of Rhodobacter sphaeroides in alcohol metabolism. - Characterized the origin and life cycle of a Bacillus amyloliquefaciens' bacteriophage.

TEACHING University of Wisconsin-Madison January 2000-May 2001 EXPERIENCE: - Graduate teaching assistant, Advanced Bacterial Physiology Laboratory for 4th year undergraduates majoring in microbiology. - Graduate teaching assistant, Introduction to Bacteriology Laboratory for 1st & 2nd year undergraduates majoring in microbiology

University of Wisconsin-LaCrosse January 1998-May 1998 - Teaching Assistant, Microbial Physiology Lecture and Laboratory for 4th year undergraduates.

LEADERSHIP - Mentor of 6 undergraduate/summer researchers May 2000-present ROLES: - UW-Madison Microbiology Program Steering Committee August 2002-July 2003 - UW-Madison Microbiology Program Recruiting Chair August 2000-July 2002

PUBLICATIONS: Anthony, J.R., Ho, K., and J.D. Keasling. 2006. Improvements in the terpene synthase amorpha-4,11-diene synthase result in increased amorphadiene production. (In preparation).

Anthony, J.R., Nowroozi, F., Anthony, L.C., Kwon, G., and J.D. Keasling. 2006. Improvements in pathway engineering for the production of the anti-malarial precursor amorpha-4,11-diene in E. coli. (In preparation).

Anthony, J.R., Warczak, K.L., and T.J. Donohue. 2005. A transcriptional response to a toxic byproduct of photosynthesis. PNAS. 102:6502-6507.

Anthony, J.R., Newman, J., and T.J. Donohue. 2004. Interactions between the Rhodobacter sphaeroides ECF sigma factor, √E, and its anti-sigma factor, ChrR. J Mol Biol. 341:345-360.

Anthony, J.R., Green, H., Newman, J., and T.J. Donohue. 2003. Purification of Rhodobacter sphaeroides RNA polymerase and its sigma factors. Methods Enzymol. 370:54-65.

Newman, J.D., Anthony, J.R., and T.J. Donohue. 2001. The importance of zinc-binding to the function of Rhodobacter sphaeroides ChrR as an anti-sigma factor. J Mol Biol. 313:485-499.

Jennifer R. Anthony

Hirvonen, C.A., Ross, W., Wozniak, C.E., Marasco, E., Anthony, J.R., Aiyar, S.E., Newburn, V.H., and R.L. Gourse. 2001. Contributions of UP elements and the transcriptional factor FIS to expression from the seven rrn P1 promoters in Escherichia coli. J Bacteriol. 183:6305-6314.

Karls, R.K., Wolf, J.R., and T.J. Donohue. 1999. Activation of the cycA P2 promoter for the Rhodobacter sphaeroides cytochrome c2 gene by the photosynthesis response regulator. Mol Microbiol. 34:822-835.

CONFERENCE PRESENTATIONS: “The effects of singlet oxygen on the activity of Rhodobacter sphaeroides E.”Anthony, J.R., and T.J. Donohue. Poster/Abstract. 2004 Gordon Conference on Microbial Stress Response.

“Characterization of Rhodobacter sphaeroides √E mutants altered for interactions with the anti-sigma factor, ChrR.” Anthony, J.R., and T.J. Donohue. Poster/Abstract. 2003 Molecular Genetics of Bacteria and Phages Meeting.

“Characterization of Rhodobacter sphaeroides √E mutants altered for interactions with the anti-sigma factor, ChrR.” Anthony, J.R., and T.J. Donohue. Poster/Abstract. 2003 FASEB Meeting.

“Identifying regions of Rhodobacter sphaeroides √E required for interaction with the anti-sigma factor ChrR.” Anthony, J.R., and T.J. Donohue. Poster/Abstract. 2002 Molecular Genetics of Bacteria and Phages Meeting.

“Identifying regions of Rhodobacter sphaeroides √E required for interaction with the anti- sigma factor ChrR.” Anthony, J.R., and T.J. Donohue. Poster/Abstract. 2001 FASEB Meeting

“In vitro and in vivo activation of the anaerobically-induced promoter, cycA P2, for the Rhodobacter sphaeroides cyctochrome c2 gene by the photosynthesis response regulator.” Karls, R.K., Anthony, J.R., and T.J. Donohue. Poster/Abstract. 1999 American Society for Microbiology Annual Meeting.