Libby JM, Quantz C; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8; 97: 546 (abstract no. U15).
Molecular Devices Corporation, Sunnyvale, CA.
A microphysiometer (a device which measures the metabolic activity of cells) was used to determine the response of two Mycobacterium species to antibiotics. The effects of isonicotinic acid hydrazide (INH) on an INH-sensitive strain of M. smegmatis was apparent after 4.5 h of growth in the microphysiometer. The metabolism of an INH-resistant strain continued to increase, doubling every 5.3 h. M. tuberculosis was grown for 24 h in Middlebrook 7H9 broth with and without INH, then transferred to a microphysiometer. Metabolic activities of negative controls and antibiotic-exposed populations were distinguishable after 8 h (32 h after the start of the experiment). When M. tuberculosis was exposed to streptomycin or INH for the first time in the microphysiometer, metabolism began to decline after 32 hours (streptomycin) and 38 hours (INH). These results suggest that the microphysiometer may provide a rapid means of measuring the response of mycobacteria to antimicrobial agents.
Publication Types:
Keywords:
- Anti-Bacterial Agents
- Isoniazid
- Mycobacterium
- Mycobacterium Infections
- Streptomycin
- Tuberculosis
Other ID:
UI: 102235431
From Meeting Abstracts