Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Additive effects of glucagon and non-metabolizable analogue of leucine on glutamine catabolism in rat hepatocytes |
| Creator/Author | Zaleski, J. ; Erecinska, M. |
| Publication Date | 1986 May 01 |
| OSTI Identifier | OSTI ID: 5018643 |
| Report Number(s) | CONF-8606151- |
| Other Number(s) | CODEN: FEPRA |
| Resource Type | Conference |
| Specific Type | Journal Article |
| Resource Relation | Fed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:6; 76. annual meeting of the Federation of American Society for Experimental Biology; 8 Jun 1986; Washington, DC, USA |
| Research Org | Univ. of Pennsylvania Medical School, Philadelphia |
| Subject | 550501 -- Metabolism-- Tracer Techniques; AMIDASES-- CHEMICAL ACTIVATION;GLUCAGON-- BIOLOGICAL EFFECTS;GLUTAMINE-- CATABOLISM; CARBON 14 COMPOUNDS;CARBON DIOXIDE;FASTING;GLUCOSE;LEUCINE;LIVER CELLS;ORNITHINE;RATS;TRACER TECHNIQUES |
| Related Subject | ALDEHYDES;AMIDES;AMINO ACIDS;ANIMAL CELLS;ANIMALS;CARBOHYDRATES;CARBON COMPOUNDS;CARBON OXIDES;CARBOXYLIC ACIDS;CHALCOGENIDES;ENZYMES;HEXOSES;HORMONES;HYDROLASES;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MAMMALS;METABOLISM;MONOSACCHARIDES;NON-PEPTIDE C-N HYDROLASES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;OXIDES;OXYGEN COMPOUNDS;PEPTIDE HORMONES;PEPTIDES;POLYPEPTIDES;PROTEINS;RODENTS;SACCHARIDES;SOMATIC CELLS;VERTEBRATES |
| Description/Abstract | ..beta..-2-Aminobicyclo-(2.2.1)-heptane-2-carboxylic acid (BCH), at 10 mM, a known activator of glutamate dehydrogenase, doubled the rate of urea synthesis in hepatocytes from fed rats incubated with 10 mM glutamine (gln) and 2 mM ornithine.^The stimulation of urea formation was accompanied by 1.4-fold activation of /sup 14/CO/sub 2/ release from (U-/sup 14/C)gln and 2.5-fold decline in intracellular gln content.^There was no significant change in glutamate level while 2-oxoglutarate and malate increased by 1.3- and 2.4-fold, respectively.^In hepatocytes from fasted rats BCH was similarly effective in stimulation of urea and /sup 14/CO/sub 2/ formation and it also doubled the rate of glucose synthesis.^Effects of BCH on hepatocytes from fasted rats were associated with about 2-fold elevation of glutamate, aspartate and alanine levels.^The effects of BCH and glucagon (a putative glutaminase activator) on urea, /sup 14/CO/sub 2/ and glucose formation were additive.^The results suggest that BCH-induced activation of gln catabolism in hepatocytes is caused primarily by stimulation of glutaminase rather than glutamate dehydrogenase.^It seems that the mechanisms of activation of glutaminase by BCH and glucagon are different. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: 1732 |
| System Entry Date | 2001 May 13 |
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