Bibliographic Citation
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Title | Additive effects of glucagon and non-metabolizable analogue of leucine on glutamine catabolism in rat hepatocytes |
Creator/Author | Zaleski, J. ; Erecinska, M. |
Publication Date | 1986 May 01 |
OSTI Identifier | OSTI ID: 5018643 |
Report Number(s) | CONF-8606151- |
Other Number(s) | CODEN: FEPRA |
Resource Type | Conference |
Specific Type | Journal Article |
Resource Relation | Fed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:6; 76. annual meeting of the Federation of American Society for Experimental Biology; 8 Jun 1986; Washington, DC, USA |
Research Org | Univ. of Pennsylvania Medical School, Philadelphia |
Subject | 550501 -- Metabolism-- Tracer Techniques; AMIDASES-- CHEMICAL ACTIVATION;GLUCAGON-- BIOLOGICAL EFFECTS;GLUTAMINE-- CATABOLISM; CARBON 14 COMPOUNDS;CARBON DIOXIDE;FASTING;GLUCOSE;LEUCINE;LIVER CELLS;ORNITHINE;RATS;TRACER TECHNIQUES |
Related Subject | ALDEHYDES;AMIDES;AMINO ACIDS;ANIMAL CELLS;ANIMALS;CARBOHYDRATES;CARBON COMPOUNDS;CARBON OXIDES;CARBOXYLIC ACIDS;CHALCOGENIDES;ENZYMES;HEXOSES;HORMONES;HYDROLASES;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MAMMALS;METABOLISM;MONOSACCHARIDES;NON-PEPTIDE C-N HYDROLASES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;OXIDES;OXYGEN COMPOUNDS;PEPTIDE HORMONES;PEPTIDES;POLYPEPTIDES;PROTEINS;RODENTS;SACCHARIDES;SOMATIC CELLS;VERTEBRATES |
Description/Abstract | ..beta..-2-Aminobicyclo-(2.2.1)-heptane-2-carboxylic acid (BCH), at 10 mM, a known activator of glutamate dehydrogenase, doubled the rate of urea synthesis in hepatocytes from fed rats incubated with 10 mM glutamine (gln) and 2 mM ornithine.^The stimulation of urea formation was accompanied by 1.4-fold activation of /sup 14/CO/sub 2/ release from (U-/sup 14/C)gln and 2.5-fold decline in intracellular gln content.^There was no significant change in glutamate level while 2-oxoglutarate and malate increased by 1.3- and 2.4-fold, respectively.^In hepatocytes from fasted rats BCH was similarly effective in stimulation of urea and /sup 14/CO/sub 2/ formation and it also doubled the rate of glucose synthesis.^Effects of BCH on hepatocytes from fasted rats were associated with about 2-fold elevation of glutamate, aspartate and alanine levels.^The effects of BCH and glucagon (a putative glutaminase activator) on urea, /sup 14/CO/sub 2/ and glucose formation were additive.^The results suggest that BCH-induced activation of gln catabolism in hepatocytes is caused primarily by stimulation of glutaminase rather than glutamate dehydrogenase.^It seems that the mechanisms of activation of glutaminase by BCH and glucagon are different. |
Country of Publication | United States |
Language | English |
Format | Pages: 1732 |
System Entry Date | 2001 May 13 |
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