A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragments.

Nucleic Acids Res. 1995 December 11; 23(23): 4928–4929.

PMCID: PMC307487

C Wawer, H Rüggeberg, G Meyer, and G Muyzer

Max-Planck-Institute for Marine Microbiology, Bremen, Germany.

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Abstract

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Selected References

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Fischer, SG; Lerman, LS. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1579–1583. [PubMed]
Rosenbaum, V; Riesner, D. Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophys Chem. 1987 May 9;26(2-3):235–246. [PubMed]
Orita, M; Iwahana, H; Kanazawa, H; Hayashi, K; Sekiya, T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766–2770. [PubMed]
Müller, W; Hattesohl, I; Schuetz, HJ; Meyer, G. Polyethylene glycol derivatives of base and sequence specific DNA ligands: DNA interaction and application for base specific separation of DNA fragments by gel electrophoresis. Nucleic Acids Res. 1981 Jan 10;9(1):95–119. [PubMed]
Müller, W; Gautier, F. Interactions of heteroaromatic compounds with nucleic acids. A - T-specific non-intercalating DNA ligands. Eur J Biochem. 1975 Jun;54(2):385–394. [PubMed]
Kruse, L; Meyer, G; Hildebrandt, A. A highly conserved repetitive sequence from Physarum polycephalum contains nucleotide arrangements similar to replicator sequences. Biochim Biophys Acta. 1993 Oct 19;1216(1):129–133. [PubMed]
Wawer, C; Muyzer, G. Genetic diversity of Desulfovibrio spp. in environmental samples analyzed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments. Appl Environ Microbiol. 1995 Jun;61(6):2203–2210. [PubMed]
STUDIER, FW. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. [PubMed]
Li, C; Peck, HD, Jr; LeGall, J; Przybyla, AE. Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas. DNA. 1987 Dec;6(6):539–551. [PubMed]
Deckers, HM; Wilson, FR; Voordouw, G. Cloning and sequencing of a [NiFe] hydrogenase operon from Desulfovibrio vulgaris Miyazaki F. J Gen Microbiol. 1990 Oct;136(10):2021–2028. [PubMed]
Rousset, M; Dermoun, Z; Hatchikian, CE; Bélaich, JP. Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe]hydrogenase from Desulfovibrio fructosovorans. Gene. 1990 Sep 28;94(1):95–101. [PubMed]