BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract

RepeatMasker Server unavailable.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= B08G12.seq(1>516) (487 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 4 Sequences     : less than 4 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 996 220 |=======================================================
   6310 776 169 |==========================================
   3980 607 133 |=================================
   2510 474 136 |==================================
   1580 338 105 |==========================
   1000 233  60 |===============
    631 173  53 |=============
    398 120  38 |=========
    251  82  24 |======
    158  58  14 |===
    100  44  10 |==
   63.1  34   8 |==
   39.8  26   7 |=
   25.1  19   5 |=
   15.8  14   5 |=
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 9  <<<<<<<<<<<<<<<<<
   10.0   9   3 |:
   6.31   6   3 |:
   3.98   3   1 |:
   2.51   2   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|9758555|dbj|BAB09056.1|(AB006703) gb|AAD20086.1~ge... +2   128  1.7e-06   1
gi|10697011|emb|CAC12722.1|(AL390738) bA438F9.2 (nove... +2    66  0.80      1
gi|10177321|dbj|BAB10647.1|(AB008269) contains simila... +2    85  0.97      1
gi|1174321|gb|AAD13517.1|pregastric lipase, LPGL {N-t... +3    62  0.99      1
gi|2136879|pir||S59904lipase, pregastric - sheep (fra... +3    62  0.99      1
gi|3859669|emb|CAA22007.1|(AL033502) hypothetical pro... +2    81  0.99      1
gi|8778814|gb|AAF79819.1|AC007396_20(AC007396) T4O12.... +2    81  0.9996    1
gi|912722|gb|AAA82849.1|(U30776) envelope glycoprotei... +3    56  0.99993   2
gi|912725|gb|AAA82851.1|(U30777) envelope glycoprotei... +3    56  0.99993   2

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|9758555|dbj|BAB09056.1|  (AB006703) gb|AAD20086.1~gene_id:MRH10.6~similar to
            unknown protein [Arabidopsis thaliana]
            Length = 450

Frame  2 hits (HSPs):                                                _____
                        __________________________________________________
Database sequence:     |                |                |                | 450
                       0              150              300

  Plus Strand HSPs:

 Score = 128 (45.1 bits), Expect = 1.7e-06, P = 1.7e-06
 Identities = 26/42 (61%), Positives = 31/42 (73%), Frame = +2

Query:     5 GRGSYQSDAPRGRFNSRNFGRGNGQDGGDRDYNKSKGNGFYR 130
             GRG Y ++APRGRF  R  GRGN QDGGD  Y + +GNG+YR
Sbjct:   409 GRGGYPTEAPRGRFGGRGSGRGN-QDGGD--Y-RPRGNGYYR 446


to_Entrezto_Relatedto_Related >gi|10697011|emb|CAC12722.1|  (AL390738) bA438F9.2 (novel protein similar to
            heterogeneous nuclear ribonucleoprotein A1 (HNRPA1)) [Homo sapiens]
            Length = 70

Frame  2 hits (HSPs):      ______________________________________         
                        __________________________________________________
Database sequence:     |             |             |              |       | 70
                       0            20            40             60

  Plus Strand HSPs:

 Score = 66 (23.2 bits), Expect = 1.6, P = 0.80
 Identities = 20/53 (37%), Positives = 25/53 (47%), Frame = +2

Query:     5 GRGSYQSDAPRGRFN--SRNFGRGNGQDGGDRDYNKSKGNGFYRPSPHQERGHSG 163
             G GSY +D   G +N  S NFG   G + G R      G G Y   P  + G+ G
Sbjct:     6 GSGSY-NDF--GNYNNQSSNFGPMKGGNFGGRSSGPCGGGGQYFAKPRNQGGYGG 57


to_Entrezto_Relatedto_Related >gi|10177321|dbj|BAB10647.1|  (AB008269) contains similarity to RNA-binding
            protein~gene_id:MSL3.12 [Arabidopsis thaliana]
            Length = 460

Frame  2 hits (HSPs):                                            _______  
                        __________________________________________________
Database sequence:     |                |               |               | | 460
                       0              150             300             450

  Plus Strand HSPs:

 Score = 85 (29.9 bits), Expect = 3.4, P = 0.97
 Identities = 25/60 (41%), Positives = 32/60 (53%), Frame = +2

Query:     8 RGSYQSDAPRGRFNSRNF--GRGNGQDGGDRDYNKSKGNGFYRP-SPHQERGHSGHHQVP 178
             RG Y S   RG F + +F  GRG G  GG   Y +  G    RP S +   G  G+ +VP
Sbjct:   382 RGRYFSG--RGSFRNESFKGGRGGGGRGG---YGRGGGEFSGRPKSSNPRNGGEGYQRVP 436

Query:   179 RNG 187
             +NG
Sbjct:   437 QNG 439


to_Entrezto_Related >gi|1174321|gb|AAD13517.1|  pregastric lipase, LPGL {N-terminal} [lambs,
            pharyngeal organs, Peptide Partial, 35 aa, segment 1 of 2]
            Length = 35

Frame  3 hits (HSPs):    ______________________________                   
                        __________________________________________________
Database sequence:     |                           |                      | 35
                       0                          20

  Plus Strand HSPs:

 Score = 62 (21.8 bits), Expect = 4.5, P = 0.99
 Identities = 10/21 (47%), Positives = 16/21 (76%), Frame = +3

Query:   183 MGRILLSPESTKNISQRIQFW 245
             +G+I  +PE++ N+SQ I FW
Sbjct:     2 LGKIAENPEASMNVSQMISFW 22


to_Entrezto_Relatedto_Related >gi|2136879|pir||S59904  lipase, pregastric - sheep (fragments)
            Length = 62

Frame  3 hits (HSPs):   _________________                                 
                        __________________________________________________
Database sequence:     |               |               |               |  | 62
                       0              20              40              60

  Plus Strand HSPs:

 Score = 62 (21.8 bits), Expect = 4.5, P = 0.99
 Identities = 10/21 (47%), Positives = 16/21 (76%), Frame = +3

Query:   183 MGRILLSPESTKNISQRIQFW 245
             +G+I  +PE++ N+SQ I FW
Sbjct:     2 LGKIAENPEASMNVSQMISFW 22


to_Entrezto_Relatedto_Related >gi|3859669|emb|CAA22007.1|  (AL033502) hypothetical protein [Candida albicans]
            Length = 263

Frame  2 hits (HSPs):                              ____________           
                        __________________________________________________
Database sequence:     |         |        |         |        |         |  | 263
                       0        50      100       150      200       250

  Plus Strand HSPs:

 Score = 81 (28.5 bits), Expect = 4.6, P = 0.99
 Identities = 22/55 (40%), Positives = 31/55 (56%), Frame = +2

Query:     5 GRGSYQSDAPRGRFNS-RNFGRGNGQDGGDRDYNKSKG--NGFYRPSPHQERGHSGH 166
             GRG Y+ D  RG +   R+ GRG G  GG RD  +  G  +G YR   +++ G  G+
Sbjct:   147 GRGGYR-DGGRGGYGGYRDGGRGGGY-GGYRDGGRGGGYRDGGYRDGGYRDGGRGGY 201


to_Entrezto_Relatedto_Related >gi|8778814|gb|AAF79819.1|AC007396_20  (AC007396) T4O12.22 [Arabidopsis
            thaliana]
            Length = 369

Frame  2 hits (HSPs):                              _________              
                        __________________________________________________
Database sequence:     |                    |                   |         | 369
                       0                  150                 300

  Plus Strand HSPs:

 Score = 81 (28.5 bits), Expect = 7.8, P = 1.0
 Identities = 21/61 (34%), Positives = 32/61 (52%), Frame = +2

Query:    14 SYQSDAPRGRF-NSRNFGRGNGQDGGDRDYNKSKGNGFYRPSPHQERGHS--GHHQVPRN 184
             SY     RGR  +SR  GRG G +G   +Y+  +  G+   +PH+ RG+   G +  P  
Sbjct:   206 SYGRGRGRGRGRSSRGRGRG-GYNGPPNEYDAPQDGGYGYDAPHEHRGYDDRGGYDAPPQ 264

Query:   185 GQ 190
             G+
Sbjct:   265 GR 266


to_Entrezto_Relatedto_Related >gi|912722|gb|AAA82849.1|  (U30776) envelope glycoprotein gp41 [Human
            immunodeficiency virus type 1]
            Length = 91

Frame  3 hits (HSPs):                       _____________________         
Frame  2 hits (HSPs):          ________                                   
                        __________________________________________________
Database sequence:     |          |          |          |          |      | 91
                       0         20         40         60         80

  Plus Strand HSPs:

 Score = 56 (19.7 bits), Expect = 9.6, Sum P(2) = 1.0
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   240 FWVQIFQL*IVNFSRLDQILIFFMS*IKLFREYGWDV 350
             FWV +  L +  + RL  +L+     ++L    GWD+
Sbjct:    38 FWVDLRNLCLFLYHRLRDLLLIVTRIVELLGRRGWDL 74

 Score = 38 (13.4 bits), Expect = 9.6, Sum P(2) = 1.0
 Identities = 6/15 (40%), Positives = 12/15 (80%), Frame = +2

Query:    68 GNGQDGGDRDYNKSK 112
             G  ++GGD+D ++S+
Sbjct:    14 GIEEEGGDKDRDRSR 28


to_Entrezto_Relatedto_Related >gi|912725|gb|AAA82851.1|  (U30777) envelope glycoprotein gp41 [Human
            immunodeficiency virus type 1]
            Length = 91

Frame  3 hits (HSPs):                       _____________________         
Frame  2 hits (HSPs):          ________                                   
                        __________________________________________________
Database sequence:     |          |          |          |          |      | 91
                       0         20         40         60         80

  Plus Strand HSPs:

 Score = 56 (19.7 bits), Expect = 9.6, Sum P(2) = 1.0
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   240 FWVQIFQL*IVNFSRLDQILIFFMS*IKLFREYGWDV 350
             FWV +  L +  + RL  +L+     ++L    GWD+
Sbjct:    38 FWVDLRNLCLFLYHRLRDLLLIVTRIVELLGRRGWDL 74

 Score = 38 (13.4 bits), Expect = 9.6, Sum P(2) = 1.0
 Identities = 6/15 (40%), Positives = 12/15 (80%), Frame = +2

Query:    68 GNGQDGGDRDYNKSK 112
             G  ++GGD+D ++S+
Sbjct:    14 GIEEEGGDKDRDRSR 28


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.99

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.347   0.156   0.491  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.344   0.156   0.537  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.357   0.156   0.590  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.355   0.155   0.557  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.367   0.172   0.692  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.345   0.154   0.526  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      161       160       10.  75 3  12 22  0.10    34
                                                    30  0.099   37
   +2      0      162       161       10.  75 3  12 22  0.10    34
                                                    30  0.10    37
   +1      0      162       161       10.  75 3  12 22  0.10    34
                                                    30  0.10    37
   -1      0      162       161       10.  75 3  12 22  0.10    34
                                                    30  0.10    37
   -2      0      162       161       10.  75 3  12 22  0.10    34
                                                    30  0.10    37
   -3      0      161       161       10.  75 3  12 22  0.10    34
                                                    30  0.10    37


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  9
  No. of states in DFA:  591 (58 KB)
  Total size of DFA:  197 KB (256 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  157.85u 1.16s 159.01t  Elapsed: 00:00:34
  Total cpu time:  157.87u 1.20s 159.07t  Elapsed: 00:00:34
  Start:  Mon Feb  4 23:01:54 2002   End:  Mon Feb  4 23:02:28 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000