A Simple Purification of Indole-3-Acetic Acid and Abscisic Acid for GC-SIM-MS Analysis by Microfiltration of Aqueous Samples through Nylon

Plant Physiol. 1989 May; 90(1): 197–201.

PMCID: PMC1061698

James R. Dunlap and Gene Guinn

U.S. Department of Agriculture, Agricultural Research Service, Subtropical Agricultural Research Laboratory, Weslaco, Texas 78596

U.S. Department of Agriculture, Agricultural Research Service, Western Cotton Research Laboratory, Phoenix, Arizona 85040

This article has been cited by other articles in PMC.

Abstract

A simple procedure was developed for the partial purification of plant tissue samples to be analyzed simultaneously for indole-3-acetic acid (IAA) and abscisic acid (ABA). The procedure relies on removal of contaminants by filtration through nylon and partitioning into dichloromethane. This procedure successfully purified both IAA and ABA from muskmelon, cotton, and broccoli tissue. Twenty individual samples can be purified and methylated in 8 h for analysis of free IAA and ABA with gas chromatography-selected ion monitoring-mass spectrometry. The use of microfiltration of aqueous samples through nylon offers new opportunities for improving the efficiency of existing sample purification procedures.

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Chen, Kai-Hsien; Miller, Anita N; Patterson, Glenn W; Cohen, Jerry D. A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis. Plant Physiol. 1988 Mar;86(3):822–825. [PubMed]
Cohen, Jerry D; Baldi, Bruce G; Slovin, Janet Pernise. C(6)-[Benzene Ring]-Indole-3-Acetic Acid: A New Internal Standard for Quantitative Mass Spectral Analysis of Indole-3-Acetic Acid in Plants. Plant Physiol. 1986 Jan;80(1):14–19. [PubMed]
Cohen, Jerry D; Bausher, Michael G; Bialek, Krystyna; Buta, J George; Gocal, Gregory F W; Janzen, Loeke M; Pharis, Richard P; Reed, Arden N; Slovin, Janet P. Comparison of a Commercial ELISA Assay for Indole-3-Acetic Acid at Several Stages of Purification and Analysis by Gas Chromatography-Selected Ion Monitoring-Mass Spectrometry Using a C(6)-Labeled Internal Standard. Plant Physiol. 1987 Aug;84(4):982–986. [PubMed]
Guinn, Gene; Brummett, Donald L; Beier, Ross C. Purification and Measurement of Abscisic Acid and Indoleacetic Acid by High Performance Liquid Chromatography. Plant Physiol. 1986 Aug;81(4):997–1002. [PubMed]
Vine, John H; Noiton, Dominique; Plummer, Julie A; Baleriola-Lucas, Cristina; Mullins, Michael G. Simultaneous Quantitation of Indole 3-Acetic Acid and Abscisic Acid in Small Samples of Plant Tissue by Gas Chromatography/Mass Spectrometry/Selected Ion Monitoring. Plant Physiol. 1987 Oct;85(2):419–422. [PubMed]
Walker-Simmons, Mary. ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars. Plant Physiol. 1987 May;84(1):61–66. [PubMed]