ATTACHMENT I -- Progress, CY 1995

NRSP/IR BUDGET REQUESTS -- ATTACHMENT I

ACCOMPLISHMENTS CY2001

NRSP-6: Introduction, Classification, Preservation, Evaluation and Distribution of tuber-bearing Solanum species germplasm.

Introduction:

Important progress was made in building the collection. We incorporated unique germplasm from VIR (Russian) genebank, Bolivia and the USA.

Classification: Dr. Spooner continues to resolve problems in taxonomic classification that impede efficient documentation and use of the germplasm. Insights gained from these studies will allow accessions to be assigned stable species names based on empirical differences.

Preservation: The usual work for maintenance of top quality Solanum germplasm at NRSP-6 was continued. Germination tests were done on new seed and on others on a 5-year rotation. Virus tests were done on new seeds and the in vitro clonal stocks. Seed increases were done in the spring, fall and summer. RAPD markers were used to assess several aspects of the status and dynamics of genetic diversity in the genebank.

Evaluation was continued in house or with collaborators specializing in the particular trait: Combining frost tolerance with good tuber type, tuber calcium, tuber and foliar glycoalkaloids, late blight, antioxidants and hormone mutants.

Distribution: NRSP-6 distributed 6,099 units of seed, 302 tuber families and 1,197 in vitro stocks to clientele in 20 states of the United States and 15 other countries.

Intergenebank Collaboration: Research to determine the equivalence of presumed duplicate populations held in different genebanks was published and additional work to compare duplicates with respect to nematode resistance was initiated. An intergenebank meeting was planned for 2002 in Hamburg, Germany.

For specific areas of accomplishment, see Appendix listing recent publications and presentations.

NRSP/IR BUDGET REQUESTS -- ATTACHMENT II

GOALS CY2002

NRSP-6: Introduction, Classification, Preservation, Evaluation and Distribution of tuber-bearing Solanum species germplasm.

Introduction: We will continue 2001’s success in identifying elite late blight breeding stocks and test genetics of resistance. We will strengthen our collaborative ties with other genebanks. We expect to receive and assimilate materials from Bolivia and Russia.

Classification: Experiments to better understand species boundaries will continue.

Preservation: Efforts to identify less expensive, easier, and more reliable ways to grow and increase potato germplasm will continue. Samples of new germplasm will be transferred to NSSL and/or the University of Wisconsin for backup. Rigorous disease prevention and monitoring practices (mainly for viruses) will be continued. We will continue bacterial ring rot screening as a health monitoring protocol for the in vitro collection. We will continue research to measure the status and dynamics of genetic diversity in the genebank pursuant to preventing its loss during preservation.

Evaluation: We will continue evaluating potato germplasm for frost tolerance, glycoalkaloids, tuber calcium accumulation, hormone mutants, antioxidants, late blight, nematodes and other characteristics that impact the continued success of the potato crop. Evaluation is a high priority for the genebank, since it is the key to mining the value of the germplasm in which we have invested so much effort for preservation. We will continue work to find ways to manipulate tuberization for screening tuber traits.

Distribution: Potato is the world's most important vegetable crop, and the genebank at Sturgeon Bay is the world's most comprehensive and accessible collection. Germplasm and technical assistance for researchers and breeders will continue to be rapidly and impartially available here.

Intergenebank Collaboration: The cooperative intergenebank project will continue to use RAPDs to assess the dynamics of genetic diversity in model US species. We will complete work to compare reputed duplicates from the CIP and US collections. We will compare nematode resistance in the US and VIR collections.

NRSP-6 Appendix

JUSTIFICATION

For 3% SALARY increase in FY 2003

The need is increasing: The size of the collection and associated labor, supplies and upkeep are rising rapidly. This combined with the flat budgets we have received in the past several years means we are “losing ground.” We ask that this problem at least be partially offset by a 3% increase for salaries in FY 2003.

NRSP/IR BUDGET REQUESTS

SUMMARY

NRSP-6: Interregional Potato Introduction Project

	Multistate Research Funding						Other Sources of Funding
Description	Authorized FY 2001		Authorized^a FY 2002		Proposed^b FY 2003		Authorized FY 2002		Proposed^c FY 2003
	Dollars	FTE	Dollars	FTE	Dollars	FTE	Dollars	FTE	Dollars	FTE
Salaries	94,497	3.3	97,804	3.3	100,739	3.3	146,885	3.1	154,229	3.1
Fringe Benefits (Salary Only)	30,632		30,632		31,551		58,754		61,692
Wages (+ wage fringe)	3,553		3,677		3,677		0		0
Travel	6,600		6,000		6,000		8,000		8,400
Supplies	18,721		16,500		16,500		0		0
Maintenance	7,572		6,962		6,961		0		0
Equipment/Capital Imp.	0		0		0		0		0
UW Contribution (est.)	0		0		0		66,700		70,035
TOTAL	161,575		161,575		165,428		280,339		294,356

__________________

^a 3.5% salary increases

^b 3.0% increase for salaries-- see Appendix: JUSTIFICATION

^c estimated up to 5% increase

Attachment III

NRSP-6 BUDGET REQUEST

NRSP-6: Interregional Potato Introduction Project

DETAILED INFORMATION ON POSITIONS, SALARIES, AND FRINGE BENEFITS

	Multistate Research Funding						Other Sources of Funding
SALARIES	Authorized^a FY 2001		Authorized^b FY 2002		Requested FY 2003		Authorized FY 2002		Requested FY 2003
	Dol1ars	FTE	Dollars	FTE	Dollars	FTE	Dollars	FTE	Dollars	FTE
Admin. Project Assistant	25,104	0.6	25,983	0.6	26,762	0.6	17,322	0.4	17,842	0.4
Technician (Specialist)	31,224	1.0	32,317	1.0	33,286	1.0	0		0
Technician	17,082	1.0	17,680	1.0	18,210	1.0	0		0
Gardener	5,824	0.2	6,028	0.2	6,209	0.2	24,111	0.8	24,834	0.8
½ Research Assistant	15,792	0.5	16,345	0.5	16,835	0.5	0		0
Secretary / Clerical	0		0		0		16,102	0.6	16,585	0.6
ARS Research Leader	0		0		0		7,350	0.1	7,571	0.1
ARS Geneticist / Proj. Leader	0		0		0		52,000	0.8	53,560	0.8
ARS Research Botanist	0		0		0		30,000	0.4	30,900	0.4
Total Salaries	95,026		98,353		101,302		146,885		151,292
Fringe Benefits (Salaries only)	30,632		30,632		30,632		58,754		60,517
TOTAL	125,658	3.3	128,985	3.3	131,934	3.3	205,639	3.1	211,809	3.1

FY 2000-2001 % Salary increase ................................... 6.0%

FY 2002 Salary increase approved by institution = 3.5%, requested and denied RRF increase = 3.0%

FY 2003 Salary increase by institution..... at least 4.2%

^a actual

^b at 3.5% increase