Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma |
| Creator/Author | Woodworth, C.D. ; Doniger, J. ; DiPaolo, J.A. |
| Publication Date | 1989 Jan 01 |
| OSTI Identifier | OSTI ID: 6146244 |
| Other Number(s) | CODEN: JOVIA |
| Resource Type | Journal Article |
| Resource Relation | J. Virol. ; Vol/Issue: 63:1 |
| Research Org | National Cancer Institute, Bethesda, MD (USA) |
| Subject | 550701 -- Microbiology-- Tracer Techniques; CARCINOMAS-- PATHOGENESIS;DNA-- AUTORADIOGRAPHY;SOMATIC CELLS-- CELL TRANSFORMATIONS;VIRUSES-- GENE RECOMBINATION; ANTIBIOTICS;GENES;KERATIN;PLASMIDS;SKIN;TRANSCRIPTION;UTERUS |
| Related Subject | ANIMAL CELLS;ANTI-INFECTIVE AGENTS;BODY;CELL CONSTITUENTS;DISEASES;DRUGS;FEMALE GENITALS;MICROORGANISMS;NEOPLASMS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;ORGANS;PARASITES;PROTEINS;SCLEROPROTEINS |
| Description/Abstract | Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes.^Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization.^Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells.^These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes.^This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: 158-164 |
| System Entry Date | 2001 May 13 |
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