Cross-reactive human blood group H-active polysaccharide from Helix pomatia: I. Detection with catfish anti-H and eel sera

Immunology. 1973 October; 25(4): 649–661.

PMCID: PMC1423146

Cross-reactive human blood group H-active polysaccharide from Helix pomatia

I. Detection with catfish anti-H and eel sera

B. A. Baldo and G. Uhlenbruck

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Abstract

A galactogen from the albumin gland of Helix pomatia precipitates with three different catfish (Tandanus tandanus) anti-H sera and with a sample of normal eel serum. The galactogen appears to lack fucose and fails to inhibit human group O erythrocyte agglutination by saline extracts of Ulex europaeus, Evonymus europaeus and Lotus tetragonolobus, but it inhibits the agglutination of group O red cells by catfish antisera and eel serum. The possible nature of the galactogen determinant involved in the reaction with the catfish and eel anti-H sera is discussed.

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Baldo, BA. Catfish antibodies to blood group substances. 3. Further studies with sera from fish injected with human group O secretor fluids. Immunology. 1972 Jun;22(6):981–990. [PubMed]
Baldo, BA. Catfish antibodies to blood group substances. II. Cross-reacting anti-A and B antibodies. Vox Sang. 1972;22(3):244–253. [PubMed]
Baldo, BA; Boettcher, B. Natural erythrocyte agglutinins in the serum of the Australian freshwater catfish, Tandanus tandanus (Mitchell). I. Examination of the specificities of the agglutinins with emphasis on the ABH agglutinins. Immunology. 1970 Oct;19(4):569–581. [PubMed]
Bezkorovainy, A; Springer, GF; Desai, PR. Physicochemical properties of the eel anti-human blood-group H(O) antibody. Biochemistry. 1971 Sep 28;10(20):3761–3764. [PubMed]
FOTINO, M; ALOMAN, S. Diagnose des Ausscheidertypus mit pflanzlichem anti-H (Evonymus europaea). Folia Haematol (Frankf). 1959 Mar;3(2):227–235. [PubMed]
Gold, ER; Thompson, TE. Serological differences between related species of snails. II. Revealed by haemolysis tests, agglutination tests with tumour cells and content of B-like substance. Vox Sang. 1969 Feb;16(2):119–123. [PubMed]
Hammarström, S; Kabat, EA. Purification and characterization of a blood-group A reactive hemagglutinin from the snail Helix pomatia and a study of its combining site. Biochemistry. 1969 Jul;8(7):2696–2705. [PubMed]
Hammarström, S; Kabat, EA. Studies on specificity and binding properties of the blood group A reactive hemagglutinin from Helix pomatia. Biochemistry. 1971 Apr 27;10(9):1684–1692. [PubMed]
Hammarström, S; Lindberg, AA; Robertsson, ES. Precipitation of lipopolysaccharides from rough mutants of Salmonella typhimurium by an A-hemagglutinin from Helix pomatia. Eur J Biochem. 1972 Feb 15;25(2):274–282. [PubMed]
KABAT, EA. Structural similarities of methylated D- and L-fucose derivatives as seen in three-dimensional models. Biochem J. 1962 Nov;85:291–293. [PubMed]
KUHN, R; GHALEB OSMAN, H. Uber die serologische Spezifität von D- und L-Fucosiden. Hoppe Seylers Z Physiol Chem. 1956 Feb 7;303(1-2):1–8. [PubMed]
Lloyd, KO; Kabat, EA. Some products of the degradation of blood group substances by alkaline borohydride. Biochem Biophys Res Commun. 1964 Jul 27;16(5):385–390. [PubMed]
Matsumoto, I; Osawa, T. Purification and characterization of an anti-H(O) phytohemagglutinin of Ulex europeus. Biochim Biophys Acta. 1969 Nov 11;194(1):180–189. [PubMed]
MASAMUNE, H; HAKOMORI, S; KAMIYAMA, S; SATAKE, M; HASIZUME, K. Synthesis of an artificial group O substance. Tohoku J Exp Med. 1957 Feb 25;65(2-3):157–167. [PubMed]
MAY, F; WEINLAND, H. Darstellung von l-Galaktose aus Galaktogen. Hoppe Seylers Z Physiol Chem. 1954;296(3-4):154–166. [PubMed]
MORGAN, WTJ; WATKINS, WM. The inhibition of the haemagglutinins in plant seeds by human blood group substances and simple sugars. Br J Exp Pathol. 1953 Feb;34(1):94–103. [PubMed]
Pardoe, GI; Uhlenbruck, G; Bird, GW. Studies on some heterophile receptors of the Burkitt EB2 lymphoma cell. Immunology. 1970 Jan;18(1):73–83. [PubMed]
Prokop, O; Rackwitz, A; Schlesinger, D. A "new" human blood group receptor Ahel tested with saline extracts from Helix hortensis (garden snail). J Forensic Med. 1965 12(3):108–110.Jul–Sep; [PubMed]
Prokop, O; Uhlenbruck, G; Köhler, W. A new source of antibody-like substances having anti-blood group specificity. A discussion on the specificity of Helix agglutinins. Vox Sang. 1968;14(5):321–333. [PubMed]
REGE, WP; PAINTER, TJ; WATKINS, WM; MORGAN, WT. ISOLATION OF SEROLOGICALLY ACTIVE FUCOSE-CONTAINING OLIGOSACCHARIDES FROM HUMAN BLOOD-GROUP H SUBSTANCE. Nature. 1964 Jul 25;203:360–363. [PubMed]
RENNER, DHP; WEINLAND, H. Der Anfangsverlauf der Galaktogenhydrolyse. Hoppe Seylers Z Physiol Chem. 1957;309(1-3):52–59. [PubMed]
Schnitzler, S; Krüger, W; Uhlenbruck, G. Hämagglutinine von Helix lucorum und Caucasotachea atrikabuata: Vergleichende Untersuchungen zu Anti-AHP UND Anti-ACN. Z Immunitatsforsch Allerg Klin Immunol. 1970 Aug;140(2):156–167. [PubMed]
Springer, GF; Desai, PR. Monosaccharides as specific precipitinogens of eel anti-human blood-group H(O) antibody. Biochemistry. 1971 Sep 28;10(20):3749–3761. [PubMed]
SPRINGER, GF; DESAI, PR; KOLECKI, B. SYNTHESIS AND IMMUNOCHEMISTRY OF FUCOSE METHYL ETHERS AND THEIR METHYLGLYCOSIDES. Biochemistry. 1964 Aug;3:1076–1085. [PubMed]
SPRINGER, GF; WILLIAMSON, P. Immunochemical significance of L- and D-fucose derivatives. Biochem J. 1962 Nov;85:282–291. [PubMed]
SPRINGER, GF; WILLIAMSON, P. Limitations of heterologous reagents in the elucidation of blood group H(O) specific structures. Vox Sang. 1963 8:177–195.Mar–Apr; [PubMed]
Uhlenbruck, G; Gielen, W. Serologische Eigenschaften von Ovalbumin nach Kupplung mit diazotiertem p-Amino-phenyl-N-acetyl-beta-galaktosaminid: Ein Beitrag zur Spezifität von Anti-Ahel. Hoppe Seylers Z Physiol Chem. 1967 Dec;348(12):1693–1696. [PubMed]
Uhlenbruck, G; Prokop, O. An agglutinin from Helix pomatia, which reacts with terminal N-acetyl-D-galactosamine. Vox Sang. 1966 11(4):519–520.Jul–Aug; [PubMed]
Voigtmann, R; Salfner, B; Uhlenbruck, G. Studies on broad spectrum agglutinins. IX. Specific and unspecific reactions between Limulus polyphemus haemolymph and snail extracts with "anti-A" specificity. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):488–494. [PubMed]
WATKINS, WM; MORGAN, WT. Neutralization of the anti-H agglutinin in eel serum by simple sugars. Nature. 1952 May 17;169(4307):825–826. [PubMed]
MORGAN, WT; WATKINS, WM. Role of a D-glucosamine as inhibitor of the precipitation of blood group substances by anti-type XIV pneumococcus serum. Nature. 1956 Dec 8;178(4545):1289–1290. [PubMed]
WATKINS, WM; MORGAN, WT. Further observations on the inhibition of blood-group specific serological reactions by simple sugars of known structure. Vox Sang. 1962;7:129–150. [PubMed]
WEINLAND, H. Beobachtungen bei der Säurehydrolyse des Galaktogens. II. Isolierung und Nachweis der auftretenden Disaccharide 3beta-D-Galaktosido-D-galaktose und 6beta-D-Galaktosido-D-galaktose mit Spuren 6alpha-L-Galaktosido-D-galaktose. Hoppe Seylers Z Physiol Chem. 1956 Aug 8;305(2-3):87–96. [PubMed]