Sahm M, Wingen F, Seitz HM; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 384 (abstract no. PO-B10-1494).
Institute of Medical Parasitology, University of Bonn.
It was attempted to culture Pneumocystis carinii without a feedercell layer using a modification of TEGOSHI's medium (1989). Inocula for the cultures were enriched homogenates of infected lungs taken from nude rats, which usually develop Pneumocystis pneumonia spontaneously. To evaluate growth and culture dynamics the number of trophozoites and cysts which showed intracystic bodies were counted in Giemsa-stained preparations. A definite growth or multiplication of P. carinii was not seen in the cultures, but trophozoites were well preserved up to five days or even longer. To investigate whether this form of cultivation for P. carinii was suitable as an alternative system for drug evaluation, pentamidine isethionate was added to the cultures. The efficiency of this drug against P. carinii is well established. The concentration of pentamidine (0.015 mg/ml medium) chosen for the in vitro experiments corresponded approximately to the pentamidine concentration, which can be reached in alveolar lumina of patients by inhalation of 300 mg in 6 ml aqua distillate. In spite of the fact that no definitive growth of P. carinii was observed, the system is, nevertheless, suitable for drug evaluation. The application of pentamidine isethionate led to an defined decrease in the number of trophozoites (70% inhibition) compared to control cultures in which the trophozoites were well preserved up to five days.
Publication Types:
Keywords:
- Animals
- Azure Stains
- Culture
- Disease Models, Animal
- Drug Evaluation
- Growth
- Human Body
- Humans
- In Vitro
- Lung
- Models, Biological
- Organ Size
- Pentamidine
- Pneumocystis carinii
- Pneumonia, Pneumocystis
- Rats, Nude
- Substance Abuse Detection
Other ID:
UI: 102204429
From Meeting Abstracts