| US 7,387,875 B2 | ||
| Methods for monitoring multiple gene expression | ||
| Randy Berka, Davis, Calif. (US); Elena Bachkirova, Davis, Calif. (US); and Michael Rey, Davis, Calif. (US) | ||
| Assigned to Novozymes, Inc., Davis, Calif. (US) | ||
| Filed on Sep. 24, 2004, as Appl. No. 10/950,009. | ||
| Claims priority of provisional application 60/506140, filed on Sep. 25, 2003. | ||
| Prior Publication US 2005/0069934 A1, Mar. 31, 2005 | ||
| Int. Cl. C12Q 1/68 (2006.01); C12N 1/00 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—6 [435/254.1; 536/23.1] | 8 Claims |
| 1. A method for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to
expression of the same genes in one or more second filamentous fungal cells, comprising:
(a) adding a mixture of detection reporter-labeled nucleic acids isolated from the first and one or more second filamentous
fungal cells to a substrate containing an array of Trichoderma reesei ESTs or SSH clones, or a combination thereof, selected from the group consisting of SEQ ID NOs. 1-1188, under conditions where
the nucleic acids hybridize to complementary sequences of the ESTs or SSH clones, or a combination thereof, in the array,
wherein the nucleic acids from the first filamentous fungal cell and the one or more second filamentous fungal cells are labeled
with a first detection reporter and one or more different second detection reporters, respectively; and
(b) examining the array under conditions wherein the relative expression of the genes in the first and one or more second
filamentous fungal cells is determined by the observed detection signal of each spot in the array in which (i) the Trichoderma reesei ESTs or SSH clones, or a combination thereof, in the array that hybridize to the nucleic acids obtained from either the first
or the one or more second filamentous fungal cells produce a distinct first detection signal or one or more second detection
signals, respectively, and (ii) the Trichoderma reesei ESTs or SSH clones, or a combination thereof, in the array that hybridize to the nucleic acids obtained from both the first
and one or more second filamentous fungal cells produce a distinct combined detection signal;
wherein the hybridization conditions are selected from the group consisting of very low, low, low-medium, medium, medium-high,
high, and very high stringency conditions.
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