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Title Secretin: specific binding to rat brain membranes
Creator/Author Fremeau, R.T. Jr. ; Jensen, R.T. ; Charlton, C.G. ; Miller, R.L. ; O`Donohue, T.L. ; Moody, T.W.
Publication Date1983 Aug 01
OSTI IdentifierOSTI ID: 5478113
Other Number(s)CODEN: JNRSD
Resource TypeJournal Article
Resource RelationJ. Neurosci. ; Vol/Issue: 3:8
Research OrgDepartment of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, DC
Subject550201 -- Biochemistry-- Tracer Techniques ;551001 -- Physiological Systems-- Tracer Techniques; ;CEREBELLUM-- RECEPTORS;CEREBRAL CORTEX-- RECEPTORS;HIPPOCAMPUS-- RECEPTORS;RECEPTORS-- SPECIFICITY;SECRETIN-- RADIORECEPTOR ASSAY;SECRETIN-- RECEPTORS;THALAMUS-- RECEPTORS; BIOCHEMICAL REACTION KINETICS;CELL MEMBRANES;GUANINE;HYPOTHALAMUS;IODINE 125;NUCLEOTIDES;RATS
Related SubjectAMINES;ANIMALS;AROMATICS;AZAARENES;BETA DECAY RADIOISOTOPES;BODY;BRAIN;CELL CONSTITUENTS;CENTRAL NERVOUS SYSTEM;CEREBRUM;DAYS LIVING RADIOISOTOPES;ELECTRON CAPTURE RADIOISOTOPES;HETEROCYCLIC COMPOUNDS;HORMONES;HYDROXY COMPOUNDS;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;KINETICS;MAMMALS;MEMBRANES;NERVOUS SYSTEM;NUCLEI;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;ORGANS;PURINES;RADIOISOTOPES;REACTION KINETICS;RODENTS;TRACER TECHNIQUES;VERTEBRATES
Description/Abstract The binding of (/sup 125/I)secretin to rat brain membranes was investigated.^Radiolabeled secretin bound with high affinity (KD .^0.2 nM) to a single class of noninteracting sites.^Binding was specific, saturable, and reversible.^Regional distribution studies indicated that the specific binding was greatest in the cerebellum, intermediate in the cortex, thalamus, striatum, hippocampus, and hypothalamus, and lowest in the midbrain and medulla/pons.^Pharmacological studies indicated that only secretin, but not other peptides, inhibits binding of (/sup 125/I)secretin with high affinity.^Also, certain guanine nucleotides inhibited high affinity binding.^These data indicate that rat brain membranes possess high affinity binding sites specific for secretin and that with the use of (/sup 125/I) secretin the kinetics, stoichiometry, specificity, and distribution of secretin receptors can be directly investigated.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1620-1625
System Entry Date2001 May 13

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