RFE Fish Species Identification Isoelectric Focusing Tools - Arrowtooth Flounder PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Atheresthes stomias
FDA Seafood List Common Name: Arrowtooth Flounder
FDA Seafood List Market Name: Flounder, Arrowtooth
Unique IEF Gel Code: 19930813gel03
Unique Web Code: arg01Srr
Tissue Aliquot Code: athestom 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.2	0.0955037	11	1.72226	18.6	13.6	3.5	49.7	10.9	0.0888228
2	4.55	195	17.1	0.036014	12	0.722388	7.8	5.7	4.55	193.5	12.1	0.0335579
3	5.2	268	23.6	0.0442984	9	0.726598	7.8	5.7	5.2	270.8	9.4	0.0438307
4	5.85	423	37.2	0.177593	4	1.4167	15.3	11.2	5.85	422.6	4.5	0.17817
5	6.55	579	50.9	0.100915	6	0.979486	10.6	7.7	6.55	577.7	6.1	0.0910034
6	6.85	617	54.2	0.0277325	4	0.18375	2	1.5	6.85	617.4	3.9	0.0266334
7	7.35	711	62.5	0.0409814	6	0.367106	4	2.9	7.35	710.6	5.5	0.0375464
8	8.15	884	77.7	0.0302227	10	0.492024	5.3	3.9	8.15	884.3	9.7	0.0287475
9	8.45	946	83.1	0.0450066	8	0.635558	6.9	5	8.45	944.7	8.2	0.0437888
10	8.65	984	86.5	0.024182	7	0.279027	3	2.2	8.65	983.4	7.1	0.0222303
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1114	97.9	0.146104	8	1.73706	18.8	13.7	9.3	1112.3	7.7	0.127828

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.2	0.0856215	13	1.66141	7.9	6.9	3.5	50.7	12.7	0.0739492
2	3.816	91	8	0.157241	6	1.76804	8.4	7.3	3.816	90.6	6.3	0.15926
3	4.007	117	10.3	0.214464	4	1.65615	7.9	6.9	4.007	117.6	4.3	0.219252
4	4.139	135	11.9	0.102361	3	0.645846	3.1	2.7	4.139	134.7	3.5	0.105164
5	4.55	191	16.8	0.0370051	11	0.652665	3.1	2.7	4.55	193.4	11.2	0.0330163
6	5.005	240	21.1	0.026864	6	0.275229	1.3	1.1	5.005	239.9	5.8	0.0269223
7	5.2	261	22.9	0.0348531	7	0.53441	2.5	2.2	5.2	263.7	7.4	0.0407857
8	5.283	281	24.7	0.197494	6	2.12719	10.1	8.8	5.283	280.1	5.9	0.204018
9	5.592	355	31.2	0.318162	4	2.50448	11.9	10.4	5.592	355.1	4.4	0.323975
10	5.646	368	32.3	0.148206	5	1.27428	6.1	5.3	5.646	367.3	5.1	0.142039
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	417	36.6	0.149663	4	1.16463	5.5	4.8	5.85	417.6	4.4	0.150147
12	5.977	446	39.2	0.10037	4	0.758567	3.6	3.2	5.977	447.1	4.5	0.0959136
13	6.174	491	43.1	0.0460403	4	0.333869	1.6	1.4	6.174	491.2	4.4	0.0423774
14	6.253	509	44.7	0.0598724	5	0.465782	2.2	1.9	6.253	507.9	4.7	0.0553773
15	6.392	541	47.5	0.0116054	3	0.0684499	0.3	0.3	6.392	540.9	3.2	0.0119672
16	6.55	577	50.7	0.0635189	7	0.699515	3.3	2.9	6.55	574.6	6.6	0.0602051
17	6.773	606	53.2	0.0296231	2	0.103994	0.5	0.4	6.773	605.5	1.9	0.030957
18	6.85	616	54.1	0.0266579	5	0.228944	1.1	1	6.85	615.6	5.4	0.0239505
19	7.35	708	62.2	0.0358984	6	0.334281	1.6	1.4	7.35	707.5	5.9	0.0319082
20	8.15	876	76.9	0.0219438	19	1.16041	5.5	4.8	8.15	864.1	18.8	0.034808
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	934	82	0.0379816	8	0.52658	2.5	2.2	8.45	934.8	8.2	0.0363706
22	8.65	975	85.6	0.0232151	7	0.275616	1.3	1.1	8.65	975.7	6.9	0.0226469
23	9.3	1108	97.3	0.159876	7	1.81634	8.6	7.5	9.3	1106.5	7	0.145923

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.814	92	8.1	0.116917	7	1.36569	12.9	9.7	3.814	92.1	6.5	0.118533
2	4.009	119	10.4	0.185609	5	1.47575	13.9	10.5	4.009	119.3	4.5	0.184627
3	4.131	136	11.9	0.0799104	4	0.56114	5.3	4	4.131	136.3	4.1	0.0773326
4	5.191	266	23.4	0.0331637	8	0.401755	3.8	2.9	5.191	266.8	7.8	0.0290341
5	5.29	288	25.3	0.190992	5	1.95641	18.5	13.9	5.29	286.3	5.4	0.203503
6	5.586	357	31.3	0.370167	3	2.25495	21.3	16	5.586	357.9	3.4	0.369405
7	5.642	370	32.5	0.174216	5	1.32394	12.5	9.4	5.642	369.8	4.7	0.159872
8	5.962	444	39	0.0776689	5	0.645658	6.1	4.6	5.962	445.5	5	0.073347
9	6.15	487	42.8	0.0251971	5	0.228116	2.2	1.6	6.15	489.2	5	0.0257697
10	6.233	506	44.4	0.0429144	3	0.245104	2.3	1.7	6.233	505.8	3.2	0.0430623
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.364	536	47.1	0.0114723	3	0.0674773	0.6	0.5	6.364	535.7	2.9	0.0132372
12	6.696	597	52.4	0.0180352	2	0.0740842	0.7	0.5	6.696	597.8	2.2	0.0190391

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	49	4.3	0.100223	10	1.75485	20.7	14.7	3.5	51.7	10.5	0.0943961
2	4.55	197	17.3	0.0397688	10	0.671434	7.9	5.6	4.55	195.4	10.2	0.0369607
3	5.2	273	24	0.0457116	8	0.672653	7.9	5.6	5.2	272.7	8.4	0.045049
4	5.85	420	36.9	0.172531	4	1.11936	13.2	9.4	5.85	420	3.7	0.172233
5	6.55	580	50.9	0.0835491	6	0.761968	9	6.4	6.55	579.1	5.7	0.0754515
6	6.85	617	54.2	0.0251068	5	0.195172	2.3	1.6	6.85	617.1	4.6	0.0238392
7	7.35	710	62.3	0.0319971	6	0.314144	3.7	2.6	7.35	709	6	0.0296969
8	8.15	878	77.1	0.0273538	11	0.485976	5.7	4.1	8.15	877.8	10.9	0.0250647
9	8.45	938	82.4	0.0406437	6	0.464535	5.5	3.9	8.45	938.4	6.5	0.0403809
10	8.65	975	85.6	0.0234263	7	0.265062	3.1	2.2	8.65	975.1	6.7	0.0222485
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1105	97	0.143848	8	1.78229	21	15	9.3	1102.6	7.6	0.131815

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	39	3.4	0.10101	11	1.88717	7.8	6.7	3.5	41.9	11.2	0.095378
2	3.867	90	8	0.172827	5	1.66611	6.9	5.9	3.867	90.2	5.4	0.174723
3	4.032	113	10	0.20059	4	1.56519	6.5	5.6	4.032	113.2	4.4	0.20099
4	4.154	130	11.5	0.0888046	4	0.630065	2.6	2.2	4.154	129.7	4	0.0891947
5	4.55	185	16.3	0.0406042	9	0.650099	2.7	2.3	4.55	185.7	9.4	0.038904
6	5.01	236	20.8	0.0257895	8	0.353864	1.5	1.3	5.01	237.6	7.6	0.0262835
7	5.2	257	22.7	0.0530534	8	0.809438	3.4	2.9	5.2	256.4	8.3	0.0548623
8	5.269	273	24.1	0.194275	6	2.11515	8.8	7.5	5.269	273.3	6	0.197798
9	5.586	346	30.6	0.357643	4	2.65908	11	9.4	5.586	346.6	4.2	0.361106
10	5.642	359	31.7	0.173953	5	1.54989	6.4	5.5	5.642	358.8	5.4	0.163044
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	407	36	0.144813	4	0.974823	4	3.5	5.85	407.2	3.7	0.147811
12	5.964	433	38.3	0.118859	4	0.719674	3	2.6	5.964	434	3.6	0.114113
13	6.169	480	42.4	0.0575282	4	0.375998	1.6	1.3	6.169	480.8	4.3	0.0494066
14	6.253	499	44.1	0.0671022	5	0.536862	2.2	1.9	6.253	497.7	5.2	0.0584577
15	6.388	530	46.8	0.00982811	5	0.0861442	0.4	0.3	6.388	531.4	4.5	0.0107127
16	6.55	567	50.1	0.0714169	5	0.626119	2.6	2.2	6.55	565.9	5.2	0.0684208
17	6.771	595	52.6	0.0288157	3	0.142694	0.6	0.5	6.771	595.1	3.1	0.0261481
18	6.85	605	53.4	0.0311494	6	0.333263	1.4	1.2	6.85	606	6	0.0312278
19	7.35	698	61.7	0.0550183	6	0.567537	2.4	2	7.35	696.8	6.3	0.0509002
20	8.15	855	75.5	0.273954	4	2.13914	8.9	7.6	8.15	853.9	4.3	0.281487
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	923	81.5	0.0433145	7	0.51115	2.1	1.8	8.45	923.3	7.1	0.0408189
22	8.65	967	85.4	0.0354785	7	0.394791	1.6	1.4	8.65	966.4	6.8	0.0329259
23	9.3	1094	96.6	0.223473	8	2.80548	11.6	10	9.3	1092.3	8	0.197548

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.827	81	7.2	0.145737	6	1.49714	13	9.8	3.827	81.2	5.8	0.146172
2	4.019	107	9.5	0.189624	4	1.38775	12	9	4.019	106.8	4.1	0.190814
3	4.146	124	11	0.0732059	3	0.463264	4	3	4.146	124	3.5	0.0752155
4	5.174	250	22.1	0.0282559	5	0.249032	2.2	1.6	5.174	250.4	5.5	0.0256564
5	5.27	269	23.8	0.242483	5	2.18758	19	14.3	5.27	270.3	4.9	0.250403
6	5.583	341	30.2	0.459532	3	2.68886	23.3	17.5	5.583	341.3	3.4	0.443136
7	5.635	353	31.2	0.230045	4	1.48728	12.9	9.7	5.635	352.7	4	0.208864
8	5.952	426	37.7	0.0471045	6	0.605947	5.3	3.9	5.952	429	6.5	0.0527515
9	6.138	469	41.5	0.0232982	6	0.263557	2.3	1.7	6.138	469.4	6.2	0.0240027
10	6.216	487	43.1	0.0668459	4	0.42134	3.7	2.7	6.216	486.9	3.7	0.0638182
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.346	517	45.8	0.0230767	5	0.172639	1.5	1.1	6.346	517	5.1	0.0191611
12	6.675	580	51.3	0.0253871	2	0.0927047	0.8	0.6	6.675	579.7	2.1	0.0254258

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.131562	10	2.22212	20.9	15.3	3.5	35.5	10	0.12506
2	4.55	171	15.1	0.055319	9	0.831034	7.8	5.7	4.55	172.1	9.2	0.0508564
3	5.2	248	22	0.0460091	9	0.708844	6.7	4.9	5.2	249.3	9	0.0444808
4	5.85	397	35.2	0.163397	5	1.30338	12.2	9	5.85	396.8	4.6	0.161546
5	6.55	560	49.6	0.118593	4	0.830338	7.8	5.7	6.55	559.7	4.2	0.111664
6	6.85	599	53.1	0.0474906	6	0.460146	4.3	3.2	6.85	599.3	6.1	0.042562
7	7.35	692	61.3	0.0628936	6	0.60598	5.7	4.2	7.35	690.7	5.7	0.0596315
8	8.15	859	76.1	0.0331866	9	0.498559	4.7	3.4	8.15	861.1	8.6	0.0325703
9	8.45	923	81.8	0.0495656	7	0.619478	5.8	4.3	8.45	922.2	7.4	0.0471419
10	8.65	963	85.3	0.0374932	7	0.432951	4.1	3	8.65	961.2	7	0.0347259
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1089	96.5	0.172773	8	2.14012	20.1	14.7	9.3	1087.1	7.9	0.152434

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 18.600 4.550 7.800 5.200 7.800 5.850 15.300 6.550 10.600 6.850 2.000 7.350 4.000 8.150 5.300 8.450 6.900 8.650 3.000 9.300 18.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 7.900 3.816 8.400 4.007 7.900 4.139 3.100 4.550 3.100 5.005 1.300 5.200 2.500 5.283 10.100 5.592 11.900 5.646 6.100 5.850 5.500 5.977 3.600 6.174 1.600 6.253 2.200 6.392 0.300 6.550 3.300 6.773 0.500 6.850 1.100 7.350 1.600 8.150 5.500 8.450 2.500 8.650 1.300 9.300 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 23 Return to Lane Navigator
3.814 12.900 4.009 13.900 4.131 5.300 5.191 3.800 5.290 18.500 5.586 21.300 5.642 12.500 5.962 6.100 6.150 2.200 6.233 2.300 6.364 0.600 6.696 0.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 12 Return to Lane Navigator
3.500 20.700 4.550 7.900 5.200 7.900 5.850 13.200 6.550 9.000 6.850 2.300 7.350 3.700 8.150 5.700 8.450 5.500 8.650 3.100 9.300 21.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.500 7.800 3.867 6.900 4.032 6.500 4.154 2.600 4.550 2.700 5.010 1.500 5.200 3.400 5.269 8.800 5.586 11.000 5.642 6.400 5.850 4.000 5.964 3.000 6.169 1.600 6.253 2.200 6.388 0.400 6.550 2.600 6.771 0.600 6.850 1.400 7.350 2.400 8.150 8.900 8.450 2.100 8.650 1.600 9.300 11.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 19 PeakCount: 23 Return to Lane Navigator
3.827 13.000 4.019 12.000 4.146 4.000 5.174 2.200 5.270 19.000 5.583 23.300 5.635 12.900 5.952 5.300 6.138 2.300 6.216 3.700 6.346 1.500 6.675 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: Specimen ACTUAL GEL LANE: 20 PeakCount: 12 Return to Lane Navigator
3.500 20.900 4.550 7.800 5.200 6.700 5.850 12.200 6.550 7.800 6.850 4.300 7.350 5.700 8.150 4.700 8.450 5.800 8.650 4.100 9.300 20.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 19 LANE TYPE: pI Markers ACTUAL GEL LANE: 21 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Arrowtooth Flounder ---- Go to Display Gel Lane Navigator.