Projects

Swine Viral Diseases Pathogenesis and Immunology Appropriated (D)   Accession Number: 411523

Genetic and Antigenic Characterization of North American H1 Swine Influenza Viruses Reimbursable (R)   Accession Number: 413362

Characterization of Swine Influenza Virus Isolates Reimbursable (R)   Accession Number: 414643

Study of Influenza a Viruses Specific Cooperative Agreement (S)   Accession Number: 414486

Publications

Development of a replication defective adenovirus 5 vector expressing porcine interleukin-18 and a mutated analog - (Abstract) - (02-Sep-08)

Differences in immune response of pigs challenged with a high versus low dose inoculum of porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal)

Loving, C.L., Brockmeier, S.L., Vincent, A.L., Lager, K.M., Sacco, R.E. 2008. Differences in immune response of pigs challenged with a high versus low dose inoculum of porcine reproductive and respiratory syndrome virus. Viral Immunology. 21(3):315-326.

Investigating the porcine immune reponse to Haemophilus parasuis - (Abstract) - (15-May-08)

Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica - (Proceedings/Symposium)

Brockmeier, S., Vincent, A.L., Loving, C.L., Nicholson, T.L., Sacco, R.E. 2008. Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica. In: Proceedings of the 20th International Pig Veterinary Society Congress, June 22-26, 2008, Durban, South Africa. 1:10.

Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica - (Abstract)

Brockmeier, S., Loving, C.L., Nicholson, T.L., Vincent, A.L., Sacco, R.E. 2008. Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica [abstract]. American Society for Microbiology Meeting, June 2-4, 2008, Boston, Massachusetts. 2008 CDROM.

Coinfection of Pigs with Swine Influenza Virus and Bordetella bronchiseptica - (Abstract)

Brockmeier, S., Vincent, A.L., Nicholson, T.L., Sacco, R.E., Palmer, M.V. 2007. Coinfection of Pigs with Swine Influenza Virus and Bordetella bronchiseptica [abstract]. In: United States Japan Natural Resources Animal and Avian Health Panel, December 5, 2007, Tsukuba, Japan. p. 25.

Viral Reassortment and Transmission after Coinfection of Pigs with two Swine Influenza Viruses - (Abstract)

Ma, W., Lager, K.M., Fleckenstein, E.S., Lekcharoensuk, P., Janke, B.H., Vincent, A.L., Webby, R.J., Richt, J.A. 2007. Viral reassortment and transmission after coinfection of pigs with 2 swine influenza viruses [abstract]. Conference of Research Workers in Animal Diseases Annual Meeting. Paper No. 146. p. 147.

Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine - (Peer Reviewed Journal)

Vincent, A.L., Ma, W., Lager, K.M., Janke, B.H., Webby, R.J., Garcia-Sastre, A., Richt, J.A. 2007. Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine. Vaccine. 25:7999-8009.

Virus Vaccine Research at the National Animal Disease Center: Lessons from Swine Influenza Virus and Bovine Viral Diarrhea Virus - (Abstract)

Kehrli, Jr., M.E., Vincent, A.L., Richt, J.A., Wesley, R.D., Lager, K.M., Ridpath, J.F., Kimura, K., Roth, J.A. 2007. Virus vaccine research at the National Animal Disease Center: lessons from swine influenza virus and bovine viral diarrhea virus [abstract]. The Changing Landscape of Vaccine Development: Vaccines for Global Health. The University of Texas Medical Branch, Sealy Center for Vaccine Development. p. 14

Reproduction of PMWS of high mortality with a porcine circovirus type 2-group 1 isolate - (Proceedings/Symposium)

Cheung, A.K., Lager, K.M., Gauger, P.C., Vincent, A.L., Opriessnig, T., Kehrli, Jr., M.E. 2007. Reproduction of PMWS of high mortality with a porcine circovirus type 2-group 1 isolate. In: Proceedings of the 13th International World Association of Veterinary Laboratory Diagnosticians Symposium, November 11-14, 2007, Melbourne, Australia. p. 213.

Isolation of reassortant H2N3 avian/swine influenza virus from pigs in the United States - (Abstract)

Gramer, M., Goyal, S., Patnayak, D., Lager, K., Ma, W., Vincent, A., Richt, J., Webby, R. 2007. Isolation of reassortant H2N3 avian/swine influenza virus from pigs in the United States [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Conference. p. 122.

Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine - (Peer Reviewed Journal)

Vincent, A.L., Lager, K.M., Janke, B.H., Gramer, M.R., Richt, J.R. 2008. Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine. Veterinary Microbiology. 126(4):310-323.

Comparison of the pathogenicity of porcine circovirus type 2 group 1 and group 2 isolates - (Proceedings/Symposium)

Cheung, A.K., Lager, K.M., Gauger, P.C., Vincent, A.L., Opriessnig, T. 2007. Comparison of the pathogenicity of porcine circovirus type 2 group 1 and group 2 isolates. In: Proceedings of the 5th International Symposium on Emerging and Re-Emerging Pig Diseases, June 24-27, 2007, Krakow, Poland. p. 273.

Mortality in pigs given PCV2 virus derived from DNA clones - (Peer Reviewed Journal)

Lager, K.M., Gauger, P.C., Vincent, A.L., Opriessnig, T., Kehrli, Jr., M.E., Cheung, A.K. 2007. Mortality in pigs given porcine circovirus type 2 subgroup 1 and 2 viruses derived from DNA clones. Veterinary Record. 161(12):428-429.

Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro - (Peer Reviewed Journal)

Yu, S., Vincent, A., Opriessnig, T., Carpenter, S., Kitikoon, P., Halbur, P.G., Thacker, E. 2007. Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro. Veterinary Microbiology. 123:34-42.

Coinfection with Swine Influenza Virus and Bordetella bronchiseptica in Pigs - (Abstract)

Brockmeier, S., Vincent, A.L., Sacco, R.E., Nicholson, T.L., Palmer, M.V. 2007. Coinfection with Swine Influenza Virus and Bordetella bronchiseptica in Pigs [abstract]. American Society for Microbiology Meeting, May 21-25,2007, Toronto, Canada. 2007 CD-ROM.

Pcvad Diagnosis and Emerging Diseases: Building a Systematic Approach - (Proceedings/Symposium)

Lager, K.M., Gauger, P.C., Vincent, A.L., Cheung, A.K. 2007. PCVAD diagnosis and emerging diseases: building a systematic approach. In: Proceedings of the American Association of Swine Veterinarians Annual Meeting, March 3-6, 2007, Orlando, Florida. p. 525-528.

Identification of two genotype subgroups of porcine circovirus type 2 in swine herds of the United States - (Abstract)

Cheung, A.K., Lager, K.M., Vincent, A.L. 2006. Identification of two genotype subgroups of porcine circovirus type 2 in swine herds of the United States [abstract]. Conference of Research Workers in Animal Diseases 87th Annual Meeting. Paper No. 171. p. 78.

The selected B cell population in PRRS has a naive phenotype, undiversified repertoire and unusually hydrophobic HCDR3 - (Abstract)

Butler, J.E., Weber, P., Lemke, C.D., Kimura, K., Sinkora, M., Vincent, A.L., Vorwald, A.C., Lager, K.M. 2006. The selected B cell population in PRRS has a naive phenotype, undiversified repertoire and unusually hydrophobic HCDR3 [abstract]. 2006 International PRRS Symposium. Paper No. 33.

Detection of two porcine circovirus type 2 genotypic groups in United States swine herds - (Peer Reviewed Journal)

Cheung, A.K., Lager, K.M., Kohutyuk, O.I., Vincent, A.L., Henry, S.C., Baker, R.B., Rowland, R.R., Dunham, A.G. 2007. Detection of two porcine circovirus type 2 genotypic groups in United States swine herds. Archives of Virology. 152(5):1035-1044.

Diagnostics of Severe PCVAD - (Proceedings/Symposium)

Lager, K.M., Vincent, A.L., Cheung, A.K., Gauger, P.C. 2006. Diagnostics of severe PCVAD. In: Proceedings of the 14th Annual Swine Disease conference for Practitioners, November 9-10, 2006, Ames, Iowa. p. 131-133.

Analysis of activated immune cell populations in the bronchoalveolar lavage fluid of pigs immunized and/or infected with SIV - (Abstract)

Kimura, K., Vincent, A.L., Richt, J.A., Lager, K.M., Roth, J.A., Kehrli, Jr., M.E. 2006. Effect of vaccination and/or challenge of swine influenza virus on the proportion of activated cells in bronchoalveolar lavage fluid [abstract]. Great Lakes International Imaging and Flow Cytometry Association. Paper No. 7.

Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine - (Peer Reviewed Journal)

Richt, J.A, Lekcharoensuk, P., Lager, K.M., Vincent, A.L., Loiacono, C.M., Janke, B.H., Wu, W.H., Yoon, K.J., Webby, R.J., Solorzano, A., Garcia-Sastre, A. 2006. Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine. Journal of Virology. 80(22):11009-11018.

Evaluation of Hemagglutinin Subtype 1 Swine Influenza Viruses from the United States - (Peer Reviewed Journal)

Vincent, A.L., Lager, K.M., Ma, W., Lekcharoensuk, P., Gramer, M.R., Loiacono, C., Richt, J.A. 2006. Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States. Veterinary Microbiology. 118(3-4):212-222.

Influenza Vaccine Research at the National Animal Disease Center - (Proceedings/Symposium) - (30-Jun-06)

Vaccination of Pigs Against Swine Influenza Viruses Using a Ns1-Deleted Modified Live Vaccine - (Abstract)

Richt, J.A., Lekcharoensuk, P., Lager, K.M., Vincent, A.L., Ma, W., Loiacono, C.M., Wu, W.H., Webby, R.J., Solorzano, A., Garcia-Sastre, A. 2006. Vaccination of pigs against swine influenza viruses using a NS1-deleted modified live vaccine [abstract]. 13th International Conference on Negative Strand Viruses. Paper No. 277. p. 198.

Failure of Cross-Protection by Inactivated Vaccines for Two U.S. H1 Swine Influenza Virus (Siv) Isolates - (Proceedings/Symposium)

Vincent, A.L., Lager, K.M., Gramer, M.G., Richt, J.A., Janke, B.H. 2006. Failure of cross-protection by inactivated vaccines for two U.S. H1 swine influenza virus (SIV) isolates. In: Proceedings of the 19th International Pig Veterinary Society Congress, July 16-19, 2006, Copenhagen, Denmark. p. 266.

Assay for Proliferation of Porcine Pbmc with Oregon Green 488 and Its Application for Evaluation of T Cell Responsiveness to Swine Influenza Virus - (Abstract)

Kimura, K., Vincent, A.L., Lager, K.M., Roth, J.A., Kehrli, Jr., M.E. 2006. Assay for proliferation of porcine PBMC with Oregon Green 488 and its application for evaluation of T cell responsiveness to swine influenza virus [astract]. Journal of Immunology Supplement. 176:S118. Paper No. 59.12.

Novel Swine Influenza Virus Subtype H3n1, United States - (Peer Reviewed Journal)

Lekcharoensuk, P., Lager, K.M., Vemulapalli, R., Woodruff, M., Vincent, A.L., Richt, J.A. 2006. Novel swine influenza virus subtype H3N1, United States. Emerging Infectious Diseases. 12(5):787-794.

Acute Bvdv Infections in Non Bovine Species - (Abstract)

Ridpath, J.F., Lager, K.M., Vincent, A.L., Neill, J.D., Chase, C.L., Mark, C.S. 2006. Acute BVDV infections in non-bovine species. BVDV 2006 Symposium, National Cattlemens Beef Association Annual Meeting. Paper No. 19. p. 37.

Evaluation of North American Hemagglutinin Subtype 1 Swine Influenza Isolates - (Proceedings/Symposium)

Vincent, A.L., Lekcharoensuk, P., Ma, W., Gramer, M., Richt, J.A., Lager, K.M. 2005. Evaluation of North American hemagglutinin subtype 1 swine influenza isolates. 40th United States-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health Meeting. p. 7.

Serologic and Pathogenic Evaluation of North American Hemagglutinin Subtype 1 Swine Influenza Isolates - (Proceedings/Symposium)

Vincent, A.L., Lekcharoensuk, P., Ma, W., Gramer, M., Richt, J.A., Lager, K.M. 2006. Serologic and pathogenic evaluation of North American hemagglutinin subtype 1 swine influenza isolates. In: Proceedings of the American Association of Swine Veterinarians 2006 Annual Meeting, March 5-7, 2006, Kansas City, Missouri. p. 467-470.

An Investigation of Susceptibility to Porcine Reproductive and Respiratory Syndrome Virus Between Two Genetically Diverse Commercial Lines of Pigs - (Peer Reviewed Journal)

Vincent, A.L., Thacker, B.J., Halbur, P.G., Rothschild, M.F., Thacker, E.L. 2006. An investigation of susceptibility to porcine reproductive and respiratory syndrome virus between two genetically diverse commercial lines of pigs. Journal of Animal Science. 84(1):49-57.

Evaluation of North American Hemagglutinin Subtype 1 Swine Influenza Isolates - (Abstract)

Vincent, A.L., Lekcharoensuk, P., Ma, W., Gramer, M., Richt, J.A., Lager, K.M. 2005. Evaluation of North American hemagglutinin subtype 1 swine influenza isolates [abstract]. International Congress of Virology. p. 44.

Establishment of a Pig Model for the 1930 H1n1 Swine Influenza Virus and Application of Reverse Genetics - (Abstract)

Lekcharoensuk, P., Vincent, A.L., Lager, K.M., Solorzano, A., Garcia-Sastre, A., Richt, J.A. 2005. Establishment of a pig model for the 1930 H1N1 swine influenza virus and application of reverse genetics [abstract]. International Congress of Virology. p. 42.

The in Vitro Susceptibility of Macrophages to Porcine Reproductive and Respiratory Syndrome Virus Varies Between Genetically Diverse Lines of Pigs - (Peer Reviewed Journal)

Vincent, A.L., Thacker, B.J., Halbur, P.G., Rothschild, M.F., Thacker, E.L. 2005. The in vitro susceptibility of macrophages to porcine reproductive and respiratory syndrome virus varies between genetically diverse lines of pigs. Viral Immunology. 18:506-512.