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DOI http://dx.doi.org/10.1021/jp9616775
Title Photochemistry and fluorescence emission dynamics of single molecules in solution: B-phycoerythrin
Creator/Author Wu, M. ; Goodwin, P.M. ; Ambrose, W.P. ; Keller, R.A. [Los Alamos National Lab., NM (United States)]
Publication Date1996 Oct 24
OSTI IdentifierOSTI ID: 420728
DOE Contract NumberW-7405-ENG-36
Other Number(s)JPCHAX; ISSN 0022-3654
Resource TypeJournal Article
Resource RelationJournal of Physical Chemistry ; VOL. 100 ; ISSUE: 43 ; PBD: 24 Oct 1996
Subject66 PHYSICS ;40 CHEMISTRY ; MOLECULES; DETECTION; PHOTOCHEMISTRY; FLUORESCENCE; BLEACHING; SOLUTIONS; PHOTONS; LASERS; MOLECULAR STRUCTURE; ORGANIC COMPOUNDS; PHOTOLYSIS
Description/Abstract We report the first detailed studies of the photochemistry of single molecules in aqueous solution. Photolysis of single B-phycoerythrin (B-PE) molecules, a highly fluorescent phycobiliprotein containing 34 bilin chromophores, is observed as an abrupt cessation of the fluorescence emission from individual molecules as they flow through a=40 pL probe volume. These measurements demonstrate that B-PE is photolyzed in a single photochemical step. The observation of abrupt photobleaching of single molecules could not be made in a bulk measurement because the signal would be averaged over many molecules to yield a continuous decay. Photon pair correlation measurements of the fluorescence detected from single B-PE molecules demonstrate that the molecule behaves as a single quantum system, not as a collection of 34 independent chromophores. 45 refs., 5 figs.
Country of PublicationUnited States
LanguageEnglish
Formatpp. 17406-17409 ; PL:
System Entry Date2001 May 05

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