CLUSTAL X (1.81) multiple sequence alignment Castor_oleate_hydroxylase_T098 MGGGGRMSTVITSNNSEKK-G-GSSHLKRAPHTKPPFTLGDLKRAIPPHC Lesquerella_bifxnl_oh_desat_AA MGAGGRIMVTPSSKKSE------TEALKRGPCEKPPFTVKDLKKAIPQHC AtFad2_AAM61113 MGAGGRMPVPTSSKKSE------TDTTKRVPCEKPPFSVGDLKKAIPPHC Brassica_Fad2_AAF78778 MGAGGRMQVSPPSKKSE------TDTIKRVPCETPPFTVGELKKAIPPHC Soybean_Fad2_T07688 MGAGGRTDVPPANRKSE------VDPLKRVPFEKPQFSLSQIKKAIPPHC Soybean_Fad2-1_T07687 MGLAKETTMGGRGRVAKVE-VQGKKPLSRVPNTKPPFTVGQLKKAIPPHC Maize_Fad2 MGAGGRMTEKEREKQEQLARATGGAAMQRSPVEKPPFTLGQIKKAIPPHC Castor_Fad2 -------------------------------------------------- Castor_oleate_hydroxylase_T098 FERSFVRSFSYVAYDVCLSFLFYSIATNFFPYISSPLS-YVAWLVYWLFQ Lesquerella_bifxnl_oh_desat_AA FKRSIPRSFSYLLTDITLVSCFYYVATNYFSLLPQPLSTYLAWPLYWVCQ AtFad2_AAM61113 FKRSIPRSFSYLISDIIIASCFYYVATNYFSLLPQPLS-YLAWPLYWACQ Brassica_Fad2_AAF78778 FKRSIPRSFSYLIWDIIIASCFYYVATTYFPLLPHPLS-YFAWPLYWACQ Soybean_Fad2_T07688 FQRSVLRSFSYVVYDLTIAFCLYYVATHYFHLLPGPLS-FRGMAIYWAVQ Soybean_Fad2-1_T07687 FQRSLLTSFSYVVYDLSFAFIFY-IATTYFHLLPQPFS-LIAWPIYWVLQ Maize_Fad2 FERSVLKSFSYVVHDLVIAAALLYFALAIIPALPSPLR-YAAWPLYWIAQ Castor_Fad2 -------------------------------------------------- Castor_oleate_hydroxylase_T098 GCILTGLWVIGHECGHHAFSEYQLADDIVGLIVHSALLVPYFSWKYSHRR Lesquerella_bifxnl_oh_desat_AA GCVLTGIWVIGHECGHHAFSDYQWVDDTVGFIFHSFLLVPYFSWKYSHRR AtFad2_AAM61113 GCVLTGIWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLVPYFSWKYSHRR Brassica_Fad2_AAF78778 GCVLTGVWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLVPYFSWKYSHRR Soybean_Fad2_T07688 GCILTGVWVIAHECGHHAFSDYQLLDDIVGLILHSALLVPYFSWKYSHRR Soybean_Fad2-1_T07687 GCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRR Maize_Fad2 G----------------AFSDYSLLDDVVGLVLHSSLMVPYFSWKYSHRR Castor_Fad2 -------WVMAHDCGHHAFSDYQLLDDVVGLILHSCLLVPYFSWKHSHRR ***.*. ** **: .** *:******* **** Castor_oleate_hydroxylase_T098 HHSNIGSLERDEVFVPKSKSKISWYSKYS-NNPPGRVLTLAATLLLGWPL Lesquerella_bifxnl_oh_desat_AA HHSNNGSLEKDEVFVPPKKAAVKWYVKYL-NNPLGRILVLTVQFILGWPL AtFad2_AAM61113 HHSNTGSLERDEVFVPKQKSAIKWYGKYL-NNPLGRIMMLTVQFVLGWPL Brassica_Fad2_AAF78778 HHSNTGSLERDEVFVPKKKSDIKWYGKYL-NNPLGRTVMLTVQFTLGWPL Soybean_Fad2_T07688 HHSNTGSLERDEVFVPKQKSCIKWYSKYL-NNPPGRVLTLAVTLTLGWPL Soybean_Fad2-1_T07687 HHSNTGSLDRDEVFVPKPKSKVAWFSKYL-NNPLGRAVSLLVTLTIGWPM Maize_Fad2 HHSNTGSLERDEVFVPKKKE-LPWYTPYVYNNPVGRVVHIVVQLTLGWPL Castor_Fad2 HHSNTGSLERDEVFVPKKKSSIRWYSKYL-NNPPGRIMTIAVTLSLGWPL **** ***::****** * : *: * *** ** : : . : :***: Castor_oleate_hydroxylase_T098 YLAFNVSGRPYDR-FACHYDPYGPIFSERERLQIYIADLGIFATTFVLYQ Lesquerella_bifxnl_oh_desat_AA YLAFNVSGRPYDG-FASHFFPHAPIFKDRERLQIYISDAGILAVCYGLYR AtFad2_AAM61113 YLAFNVSGRPYDG-FACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYR Brassica_Fad2_AAF78778 YLAFNVSGRPYDGGFACHFHPNAPIYNDRERLQIYISDAGILAVCYGLFR Soybean_Fad2_T07688 YLALNVSGRPYDR-FACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLFR Soybean_Fad2-1_T07687 YLAFNVSGRPYDS-FASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYR Maize_Fad2 YLATNASGRPYPR-FACHFDPYGPIYNDRERAQIFVSDAGVVAVAFGLYK Castor_Fad2 YLAFNVSGRPYDR-FACHYDPYGPIYNDRERIEIFISDAGVLAVTFGLYQ *** *.***** **.*: * .**:.:*** *:::* .:.:. : *:: Castor_oleate_hydroxylase_T098 ATMAKGLAWVMRIYGVPLLIVNCFLVMITYLQHTHPAIPRYGSSEWDWLR Lesquerella_bifxnl_oh_desat_AA YAASQGLTAMICVYGVPLLIVNFFLVLVTFLQHTHPSLPHYDSTEWEWIR AtFad2_AAM61113 YAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLR Brassica_Fad2_AAF78778 YAAAQGVASMVCFYGVPLLIVNGLLVLITYLQHTHPSLPHYDSSEWDWLR Soybean_Fad2_T07688 LAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLR Soybean_Fad2-1_T07687 VATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLK Maize_Fad2 LAAAFGVWWVVRVYAVPLLIVNAWLVLITYLQHTHPSLPHYDSSEWDWLR Castor_Fad2 LAIAKGLAWVVCVYGVPLLVVNSFLVLITFLQHTHPALPHYDSSEWDWLR : *: :: .*.****:** ** :*:***** ::*:* *:**:*:: Castor_oleate_hydroxylase_T098 GAMVTVDRDYGVLNKVFHNIADTHVAHHLFATVPHYHAMEATKAIKPIMG Lesquerella_bifxnl_oh_desat_AA GALVTVDRDYGILNKVFHNITDTHVAHHLFATIPHYNAMEATEAIKPILG AtFad2_AAM61113 GALATVDRDYXILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILG Brassica_Fad2_AAF78778 GALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILG Soybean_Fad2_T07688 GALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILG Soybean_Fad2-1_T07687 GALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILG Maize_Fad2 GALATMDRDYGILNRVFHNITDTHVAHHLFSTMPHYHAMEATKAIRPILG Castor_Fad2 GALATVDRDYGILNKVFHNITDTQVAHHLF-------------------- **:.*:**** :**:***:*:**:****** Castor_oleate_hydroxylase_T098 EYYRYDGTPFYKALWREAKECLFVEPDEGAPTQGVFWYRNKY- Lesquerella_bifxnl_oh_desat_AA DYYHFDGTPWYVAMYREAKECLYVEPDTERGKKGVYYYNNKL- AtFad2_AAM61113 DYYQFDGTPWYVAMYREAKECIYVEPDREGDKKGVYWYNNKL- Brassica_Fad2_AAF78778 EYYQFDGTPVVKAMWREAKECIYVEPDRQGEKKGVFWYNNKL- Soybean_Fad2_T07688 EYYRFDETPFVKAMWREARECIYVEPDQSTESKGVFWYNNKL- Soybean_Fad2-1_T07687 EYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY- Maize_Fad2 EYYRFDETPFVKAMWREARECIYVEPDQSTESKGVFWYNNKL- Castor_Fad2 -------------------------------------------