Liquid medium for growth of Legionella pneumophila.

J Clin Microbiol. 1980 January; 11(1): 19–21.

PMCID: PMC273308

J D Ristroph, K W Hedlund, and R G Allen

This article has been corrected. See J Clin Microbiol. 1980 March; 11(3): 308.

This article has been cited by other articles in PMC.

Abstract

The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium gave rapid cell growth and maintained the initial antigen production. The observed generation time was 99 min with a maximum cell population of 2 X 10(2) COLONY-FORMING UNITS PER ML IN APPROXIMATELY 40 H.

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Selected References

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Brenner, DJ; Steigerwalt, AG; McDade, JE. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Intern Med. 1979 Apr;90(4):656–658. [PubMed]
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