Anesthesia and Euthanasia
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Ackerman JL,
NAL Call No. QL614 J68
A quantitative examination of the reef fish assemblage at Orpheus Island, Great
Barrier Reef, contrasting clove oil and rotenone, sampled 365 individuals of 47
species with clove oil v. 536 individuals of 53 species with rotenone. Number
of species and individuals were not found to differ significantly between the
two techniques, largely due to variation among samples. Neopomacentrus bankieri (Pomacentridae) and Eviota queenslandica (Gobiidae) were the most dominant in the
samples using either technique. Although the samples appeared to be comparable,
only 31 species (45 %) in eight families were common to both techniques. Fishes
often recovered before collection when using clove oil and were not driven out
of the reef during induction to anaesthesia. Although the samples collected
with clove oil approximate the results obtained using rotenone, enclosed
rotenone stations are the preferred method for providing relatively complete
quantitative samples.
Copyright 2002 The Fisheries
Society of the
Descriptors: sampling, stock assessment, fishery surveys,
ichthyocides, toxicants, rotenone, marine ecosystems, coral reefs, Neopomacentrus
bankieri, Pomacentridae, Eviota queenslandica, Gobiidae, Pisces,
ISEW, Australia, Queensland, Great Barrier Reef, Orepheus I.
ASFA; Copyright © 2003, FAO
Aguilar NG, Palcios CAM, Ross LG
(1999) Controlled anaesthesia of Rana
catesbeiana (Shaw) and Rana pipiens (Schreber 1792) using xylocaine
delivered by spray. Aquaculture Research. 30(4):309-311
NAL Call No. SH1F8
Frog culture is an expanding industry in many parts of
the world, and research on the culture of these animals has increased in recent
years. This has inevitably led to a need for convenient handling and sedation,
sufficient to enable various procedures to be carried out humanely. The
objective of this work was to investigate the effectiveness of controlled
anaesthesia using a proprietary xylocaine spray applied to the skin in two
commercial species of frog, the Bullfrog, Rana
catesbeiana Shaw, and the Leopard frog, Rana
pipiens Schreber 1792.
Descriptors: frog culture, anaesthesia, aquaculture
development, fish handling, Rana pipiens, Rana catesbeiana,
Northern leopard frog, bullfrog
ASFA; Copyright © 2003, FAO
Allen JL (1988) Residues of
benzocaine in rainbow trout, largemouth bass, and fish meal. Progressive
Fish Culturist. 50(1):59-60
NAL Call No. 157.5 P94
Residues of the anesthetic benzocaine in muscle tissue
of rainbow trout (Salmo gairdneri)
and largemouth bass (Micropterus
salmoides) were determined after exposure of the fish to 50 mg benzocaine/L
for 15 min and withdrawal times of 0-24 h. The mean concentration of benzocaine
residues in fish sampled immediately after exposure was 14.0 mu g/g in rainbow
trout and 10.6 mu g/g in largemouth bass. Residues were below the control value
after 8 h of withdrawal in largemouth bass and near the control value after 4 h
of withdrawal in rainbow trout. Although residues of benzocaine were high in
fish immediately after exposure, the concentration declined rapidly when the
fish were held in flowing fresh water. Fish meal prepared from Pacific salmon (Oncorhynchus sp.) that had been
anesthetized with benzocaine or tricaine (MS-222) contained residues of 45.1 mu
g benzocaine/g or 47.7 mu g tricaine/g.
Descriptors: anaesthetics, ethyl aminobenzoate,
aquaculture, freshwater aquaculture, self purification, Salmo gairdneri,
Micropterus salmoides, fish culture, residues, benzocaine residues
ASFA; Copyright © 2003, FAO
Allen JL, Vang G, Steege S, Xiong
S (1994) Solubility of benzocaine in freshwater. Progressive Fish Culturist.
56(2):145-146
NAL Call No. 157.5 P94
Benzocaine is an effective general anesthetic for fish. Its solubility was
determined in waters of various hardness and pH and over a range of
temperatures (from 5 to 30°C). Variations in water hardness and pH did not
appreciably affect the solubility of benzocaine, whereas increases in
temperature increased solubility from 409 mg/L (at 5°C) to 1,118 mg/L (at
30°C).
Descriptors: anesthetics, freshwater fish, solubility, pH
effects, temperature effects, water hardness, benzocaine
ASFA; Copyright © 2003, FAO
Amend DF, Goven BA, Elliot DG
(1982) Etomidate: Effective dosages for a new fish anesthetic. Transactions
of the American Fisheries Society. 111(3):337-341
NAL Call No. 414.9 Am3
Etomidate (r-(+)-ethyl-1-(1-phenylethyl)-1
H-imidazole-5-carboxylate) is a potent and safe anesthetic for fish. The
minimum effective dose for four species of aquarium fish zebra danio Danio rerio, black tetra Gymnocorymbus ternetzi, angelfish Pterophyllum scalare, southern platyfish
Xiphophorus maculatus ranged from 2.0
to 4.0 mg/liter, and the maximum safe dose ranged from 7.0 to 20.0 mg/liter. In
general, the lower the dose, the longer the time for anesthesia, but the faster
the recovery time. At 4.0 mg/liter, fish typically entered anesthesia within 90
seconds and recovered within 40 minutes. Etomidate is more effective in
alkaline water and higher water temperature but is not affected by total
hardness. Test species varied in their ability to survive extended or repeated
exposures to the drug. Etomidate has advantages over other commonly used fish
anesthetics and should be evaluated further.
Descriptors: anesthetics, ornamental fish, Danio rerio,
Gymnocorymbus ternetzi, Pterophyllum scalare, Xiphophorus maculatus,
etomidate
ASFA; Copyright © 2003, FAO
Anderson WG, McKinley RS,
Colavecchia M (1997) The use of clove oil as an anesthetic for rainbow trout
and its effects on swimming performance. North American Journal of
Fisheries Management. 17(2):301-307
NAL Call No. SH219.N66
The only anesthetic registered in
Descriptors: swimming, anaesthetics, anesthetics, Oncorhynchus
mykiss, Eugenia aromatica, clove oil, rainbow trout
ASFA; Copyright © 2003, FAO
Anonymous (1999) New NZ
sedative. Fish Farming International. 26(3):21
NAL Call No. SH151.F5
AQUI-S is an aquatic anaesthetic approved for use in
Descriptors: fish handling, transportation, harvesting,
aquaculture techniques, food fish, anaesthetics, Australia, New Zealand,
AQUI-S, Pisces
ASFA; Copyright © 2003, FAO
Barham WT, Caiger KH, Visser JGJ
(1979) The use of benzocaine hydrochloride as fish tranquillizer and
anaesthetic in saline waters. Journal of the
Limnological Society of
The anaesthetic effects of various concentrations of
benzocaine hydrocloride were tested on Liza
macrolepis and Sarotherodon
mossambicus in sea water and diluted sea water, respectively. Induction
time for anaesthesia was negatively correlated with increasing anaesthetic
concentrations in L. macrolepis. In S. mossambicus, however, operculum
clamping appeared to be responsible for induction times increasing with
increased anaesthetic concentration. The tranquillizing effects
of low concentrations of benzocaine hydrochloride on L. macrolepis was also studied.
Descriptors: anaesthetics, saline water, Pisces, Liza
macrolepis, Sarotherodon mossambicus, Mugilidae, Cichlidae
ASFA; Copyright © 2003, FAO
Barham WT, Schoonbee HJ (1990) A
comparison of the effects of alternating current electronarcosis, rectified
current electronarcosis and chemical anaesthesia on the blood physiology of the
freshwater bream Oreochromis mossambicus (Peters). I. The effect on
blood pH, pO2, pCO2, glucose, lactate, LDH and HBDH. Comparative
Biochemistry and Physiology. 96C(2):333-338
NAL Call No. QP1 C6
A comparison was made of the effects of alternating
current electronarcosis, rectified current electronarcosis and the chemical
anaesthesia benzocaine hydrochloride on blood physiology of the freshwater
bream Oreochromis mossambicus over a
7 day period. A statistical evaluation of results, in particular of blood
glucose and lactate levels, following narcosis, suggests that the physiological
effects of alternating current electronarcosis is the least stressful of the
three types of anaesthesia. It is therefore the method of choice for stress
studies as well as other studies on this fish species.
Descriptors: haematology, fish physiology, collecting
devices, anaesthesia, Oreochromis mossambicus, comparative studies,
electric currents
ASFA; Copyright © 2003, FAO
Barham WT, Schoonbee HJ (1990) Induction
behaviour of the tilapia Oreochromis mossambicus Peters (Pisces:
Cichlidae) subjected to electronarcosis by various alternating or rectified
currents. Water S. A. 16(1):75-78
NAL Call No. TD319 A35W3
Mature tilapia Oreochromis
mossambicus were subjected to electronarcosis by alternating or rectified
currents at various voltages and frequencies and in water of various
temperatures and conductivities and their induction behaviour observed.
Responses to induction varied from no response through slight response to
vigorous response. The results support earlier findings that a 60 V, 50 Hz sine
wave is superior to rectified current for electronarcosis of tilapia.
Descriptors: fish culture, electric currents, electric
stimuli, anaesthesia, narcosis, Oreochromis
mossambicus
ASFA; Copyright © 2003, FAO
Barham WT, Schoonbee HJ, Visser
JGJ (1989) Some observations on the narcotizing ability of electric currents
on the common carp Cyprinus carpio. Onderstepoort Journal of
Veterinary Research. 56(3):215-218
NAL Call No. 41.8 On1
Some effects of alternating current electronarcosis and of rectified current
electronarcosis on Cyprinus carpio
were investigated. In all instances recovery from narcosis was accompanied by
convulsive spasms. Haemorrhaging of the gills was also observed to occur. Carp
do not appear to be suitable candidates for electronarcosis.
Descriptors: narcosis, fish physiology, anaesthesia, Cyprinus
carpio, electric currents
ASFA; Copyright © 2003, FAO
Barham WT, Schoonbee HJ, Visser
JGJ, Smit GL (1988) A comparison of red-cell fragilities of
electronarcotized and chemically anaesthetized freshwater bream, Oreochromis
mossambicus. Comparative Biochemistry and Physiology. 91A(2):241-244
NAL Call No. QP1 C6
Red cell fragilities were determined over a period of
7 days for a.c. electronarcotized,
Descriptors: erythrocytes, hematology, osmosis,
anesthetics, electric stimuli, Oreochromis mossambicus
ASFA; Copyright © 2003, FAO
NAL Call No. SF456 F7
This part of the article investigates the distribution
of cyanide throughout the fish body when cyanide is used as an anaesthetic; how
and at what dose the cyanide reaches the intestine; and the influence of food
in the stomach upon the movement of ingested cyanide. Cyanide induced
anaesthesia is the result of severe and swift oxygen depletion in the cells,
although the exact mechanism is not known.
Descriptors: digestive system, cyanides, anaesthesia,
histopathology, long-term changes, Pomacentrus violascens
ASFA; Copyright © 2003, FAO
Bernardy JA,
NAL Call No. 381 As7
A liquid chromatographic method is described for
analysis of benzocaine (BZ), a proposed fish anesthetic, in rainbow trout
plasma. Mean recoveries of BZ from plasma samples fortified at 44-10 100 ng/mL
were 96-100%. The method detection limit is 10 ng /mL, and the limit of
quantitation is 37 ng/mL. Acetylation of BZ occurs in whole blood after storage
at room temperature (i.e., 21°C) for 10 min. However, no acetylation of BZ was detected
in plasma samples held at room temperature for 4 h. Mean method precision for
plasma samples with incurred BZ residue is similar to that for fortified
samples in the same concentration range (relative standard deviations of 0.9
and 1.2%, respectively).
Descriptors: haematology, anaesthetics, Oncorhynchus
mykiss, analytical techniques
ASFA; Copyright © 2003, FAO
Bernier NJ, Randall DJ (1998) Carbon
dioxide anaesthesia in rainbow trout: effects of hypercapnic level and stress
on induction and recovery from anaesthetic treatment. Journal of Fish
Biology 52(3):621-637
NAL Call No. QL614 J68
The physiological and anaesthetic effects of three
different levels of air-saturated and buffered CO2 anaesthesia, pCO2 = 37, 78,
or 125 mmHg, were examined in cannulated rainbow trout Oncorhynchus mykiss. Complete anaesthesia (no opercular movements)
was not achieved by these hypercapnic levels after 20 min of CO2
exposure. Although increasing pCO2 reduced the induction
times to the early stages of anaesthesia, it also resulted in increasing
hyperventilatory, hypoxaemic, and acid-base disturbances. After a 10-min
recovery period, while the respiratory acidosis component of the acid-base
disturbance was corrected, there was a significant metabolic acidosis. Recovery
time was longest in the high pCO2 treatment where 33% of the
fish died. Two additional groups (pCO2 = 37 and 78 mmHg) were
exposed to an acute stress prior to the anaesthetic treatment. Stress reduced
the hypoventilatory effects of the low pCO2 treatment,
increased the recruitment of anaerobic metabolism, and prolonged recovery time.
Although the increase in plasma catecholamines elicited by the stress was small
relative to the response obtained with the anaesthetic, stress prior to CO2
anaesthesia impaired the efficiency of the treatment. Overall, our results
suggest that pCO2 levels above 37 mmHg and/or stress prior to
the anaesthesia impair the efficiency of air-saturated and buffered CO2
anaesthesia by exacerbating the hypoxaemic effects of the hypercapnic
treatment.
Descriptors: fish culture,
anaesthesia, respiration, fish physiology, Oncorhynchus mykiss, rainbow
trout
ASFA; Copyright © 2003, FAO
Billard R (1981) Effect of Some
Fish Anesthetics on Gamete Survival During Artificial Insemination of Rainbow
Trout. Progressive Fish Culturist 43(2):72-73
NAL Call No. 157.5 P94
Male and female fish are usually anesthetized during
the process of taking spawn. They are placed in a bath with added anesthetic
and removed after loss of equilibrium. This paper examines the possible
toxicity of anesthetic solutions on sperm and eggs when artificial insemination
is performed by the dilution technique. Of the three anesthetics
(Phenoxyethanol, Quinaldine, MS-222) tested in the present experiment, only
phenoxyethanol showed a toxic effect on fertilization when the concentration in
the insemination diluent was higher than 0.05%. This effect was limited to the
sperm; the anesthetic did not seem to affect the eggs. Some caution should
therefore be exercised when this anesthetic is used to immobilize fish during
spawn taking; the risk is very low because the amount of the solution
contaminating the gametes is limited and unlikely to reach toxic concentrations
( > 0.05%).
Descriptors: induced breeding, spermatozoa, eggs,
anesthetics, toxicity, Salmo gairdneri
ASFA; Copyright © 2003, FAO
Booke HE, Hollender B, Lutterbie G
(1978) Sodium bicarbonate, an inexpensive fish anesthetic for field use.
Progressive Fish Culturist. 40(1):11-13
NAL Call No. 157.5
P94
Sodium bicarbonate was tested as a fish anesthetic at combinations of
142 to 642 mg/l, pH 6. 5 to 7. 5, with rainbow trout (Salmo gairdneri), brook trout (Salvelinus
fontinalis), and carp (Cyprinus
carpio). The combination of pH 6. 5 and 642 mg/l NaHCO3 was
the most effective treatment for causing the fish, within 5 min, to cease
locomotion and slow opercular rate, but to retain reflex response to pressure
on the caudal fin. It is suggested that pH-controlled carbon dioxide release
from the sodium bicarbonate caused the anesthetic response.
Descriptors: fish culture, anaesthetics, sodium compounds,
Salmo gairdneri, carbon dioxide, Salvelinus fontinalis, Cyprinus
carpio
Bouck GR, Johnson DA (1979) Medication
inhibits tolerance to seawater in coho salmon smolts. Transactions of
the American Fisheries Society. 108(1):63-66
NAL Call No. 414.9 Am3
Applications of 10 therapeutic and two anesthetic
agents to healthy smolts of coho salmon (Oncorhynchus
kisutch) by conventional methods were followed by two different post-treatment
circumstances. In condition 1, fish were treated and then transferred directly
to
28% seawater for 10 days; in condition 2 fish were
treated and held in fresh water for 4 days before their medium was gradually
changed over a 4-hour period to 28% seawater. In condition 1, no mortality
occurred among fish treated with 2,4-D, trichlorofon, simazine, quinaldine, or
light to moderated doses of MS-222. About 10% mortality occurred among fish
treated with formalin and nifurpirinol. High mortality in seawater followed
treatments with copper sulphate, hyamine 1622, potassium permanganate,
malachite green (one protocol), and heavy doses of MS-222. In condition 2,
mortality was reduced but still high for copper sulphate and potassium
permanganate, much lower for malachite green and hyamine 1622, and zero for the
other agents. The results indicate that additional recovery time in fresh water
is necessary between some treatments and exposure to salt water.
Descriptors: salinity tolerance, drugs, smolts, mortality
causes, Oncorhynchus kisutch, juveniles, anaesthetics
ASFA; Copyright © 2003, FAO
Boulle D (1998) The
new threat looming for
ISSN: 1024-4158
Live fish capture which has been an old Chinese custom
for centuries which was only practised on culture or freshwater species,
involved keeping fish alive until eaten. The marine live fishery was born at
the same time as the advent of cyanide fishing. This practise which is highly developed
in the Western Pacific region is now making its appearance in the
Descriptors: coral reefs, reef fisheries, anaesthetics,
resource conservation, fishery policy, catching methods, cyanides, stupefying
methods, ISW, Indian Ocean, Southwest
ASFA; Copyright © 2003, FAO
Braley H, Anderson TA. (1993) Changes
in blood metabolite concentrations in response to repeated capture, anaesthesia
and blood sampling in the golden perch, Macquaria ambigua. Comparative
Biochemistry and Physiology, A. 103A(3):445-450
NAL Call No. QP1 C6
The major metabolic changes associated with repeated capture, aquarium
transfer, anaesthesia and blood sampling were investigated in an Australian
freshwater fish, the golden perch (Macquaria
ambigua). A compounded stress response was seen after repetition of the
procedure, in which the plasma glucose rose within 3 hr and amino acid
concentrations rose and the serum free fatty acids concentration fell after 24
hr. Alanine was identified as an important circulating energy store in the
stress response of golden perch. No change was noted in the serum protein,
plasma lactate or beta -hydroxybutyrate concentrations, indicating that tissue
damage and hypoxia were absent, and that degradation of free fatty acids did
not produce metabolites excess to the requirements of gluconeogenesis and the
tricarboxylic acid cycle.
Descriptors: freshwater fish, fish physiology, metabolism,
blood, fatty acids, anaesthesia, biological stress, Macquaria ambigua,
glucose, amino acids, lactate, beta -hydroxybutyrate, levels, sampling, capture
ASFA; Copyright © 2003, FAO
Brattelid T, Smith AJ (2000) Methods of positioning fish for surgery or
other procedures out of water. Laboratory
Animals. 34 (4):430-433
NAL Call No. QL55 A1L3
Descriptor: fish, surgery, techniques, animal welfare
Brown LA (1987) Recirculation
anaesthesia for laboratory fish. Laboratory Animals.
21(3):210-215
NAL Call No. QL55 A1L3
An economic reliable long-term recirculation anaesthesia system for laboratory
fish is described. Anaesthesia of channel catfish (Ictalurus punctatus) was induced within 60 s and was maintained for
up to 40 min using tricaine methanesulphonate; recovery occurred within 30-60
s. Various surgical procedures were performed on the fish. No deaths were
recorded. All water-quality parameters tested over 19 days use of the system
remained stable except for total ammonia nitrogen and, by calculation, un-ionized
ammonia which increased to a maximum of 0 multiplied by 23 mg/l.
Descriptors: anesthesia, aquaria, recirculating systems,
fish culture, Ictalurus punctatus
ASFA; Copyright © 2003, FAO
Bruecker P, Graham M (1993) The
effects of the anesthetic ketamine hydrochloride on oxygen consumption rates
and behaviour in the fish Heros (Cichlasoma) citrinellum (Guenther,
1864). Comparative Biochemistry and Physiology, C. 104C(1): 57-59
NAL Call No. QP901 C6
Heros
citrinellum (0.106 to 0.357 kg) were
injected with 30 mg/kg ketamine hydrochloride via the dorsal aorta or caudal
vein. Immediate loss of balance and complete cessation of fin movement was
observed post injection for one to 41 min. Ventilation was barely perceptible
or ceased altogether within one minute after injection. Balance returned within
57 to 263 min after injection. Oxygen consumption rate was significantly higher
during anesthesia than during control experiments, and significantly lower than
during sham treatments. The safety margin of the drug was reduced at tropical
temperatures versus temperate conditions. The usefulness of this drug in
reducing the stress of prolonged handling, such as during transport, was not
indicated. However, injectable ketamine would be useful in reducing the effects
of handling larger freshwater tropical fish over shorter time periods.
Descriptors: fish physiology, anaesthesia, pharmacology,
hazard assessment, temperature effects, Heros citrinellum, anaesthetics,
ketamine hydrochloride, oxygen consumption, behaviour, Pisces
ASFA; Copyright © 2003, FAO
Carrasco Meza S (1983) Immobilization
of carp(Cyprinus carpio), catfish (Ictalurus punctatus) and
tilapia (Tilapia mossambica) using xylocaine with sodium bicarbonate. Thesis, Universidad Nacional
Autonoma de Mexico (
Anesthetic solutions (xilocaine and xilocaine with
sodium bicarbonate) were tested on carp (Cyprinus
carpio), catfish (Ictalurus punctatus)
and tilapia (Tilapia mossambica). The
fish were immersed in the 2 solutions so that the anesthetic would penetrate
the gills by diffusion. The catfish was the most susceptible (250 mg/l.)
followed by the carp (350 mg/l.) and the tilapia (2000 mg/1.). The carp and
catfish had a similar induction period. Tilapia showed the smallest induction
period but needed the highest xilocaine concentration. Xilocaine with sodium
bicarbonate reduced the induction period in carps and in some cases in catfish
and tilapia. In tilapia the xilocaine concentration was reduced to 250 mg/l.
and 350 mg/l. An increment in the recovery period was observed in the 3
species. The use of xilocaine with sodium bicarbonate gave more practical
results.
Descriptors: anesthetics, Cyprinus carpio, Ictalurus
punctatus, Tilapia mossambica, freshwater fish,
ASFA; Copyright © 2003, FAO
Carrasco S, Sumano H,
Navarro-Fierro R (1984) The use of lidocaine-sodium bicarbonate as
anaesthetic in fish. Aquaculture 41(4):395-398
NAL Call No. SH1A6
Lidocaine and lidocaine-sodium bicarbonate mixture were evaluated as
anaesthetics for carp (Cyprinus carpio),
catfish (Ictalurus punctatus) and
tilapia (Tilapia mossambica = Oreochromis mossambicus). Although both
induced anaesthesia, the mixture was more effective for induction and recovery,
and was able to achieve the required time for the fish to be out of water.
Descriptors: anesthetics, lidocaine, animal breeding,
aquaculture, Cyprinus carpio, Ictalurus punctatus, Oreochromis mossambicus,
lidocaine, lidocaine-sodium bicarbonate
ASFA; Copyright © 2003, FAO
Cataldi E, Di Marco P, Mandich A,
Cataudella S (1998) Serum parameters of Adriatic sturgeon Acipenser
naccarii (Pisces: Acipenseriformes): effects of temperature and stress.
Comparative Biochemistry and Physiology, A. 121A(4):351-354
NAL Call No. QP1 C6
Data on the concentrations of some blood constituents
of captive Adriatic sturgeon, Acipenser
naccarii, a primitive bony fish, are reported. Serum osmolality, Na+,
K+, Cl-, Ca++, cortisol, glucose and total
protein concentrations were measured. The effects of anaesthesia, temperature,
crowding and prolonged handling stress were tested on a group of 12 4-year-old
sturgeons sampled repeatedly. The anaesthetic does of MS 222 (140 mg/l) induced
significant osmolality elevation in the sturgeon. After exposure to colder
temperature (17°C versus 25°C), cortisol and Cl- concentrations
significantly decreased. The cultured sturgeon did not seem susceptible to
crowding and prolonged handling stress, since neither the serum cortisol and
glucose levels nor the other blood parameters were affected by these stressors.
Results are compared with the few available data on other chondrostean fish and
with those on teleosts.
Descriptors: captivity, biological stress, haematology,
serological studies, temperature effects, fish culture, anaesthesia, Acipenser
naccarii, Adriatic sturgeon
ASFA; Copyright © 2003, FAO
NAL Call No. 157.5 P94
The anesthetic tricaine was used to separate normally
developing larvae of striped bass (Morone
saxatilis) from larvae with uninflated gas bladders. The procedure was most
successful between 23 and 40 d posthatch, and was used to provide thousands of
striped bass larvae in which the frequency of gas bladder inflation exceeded
99%. Few normally developing fish were lost due to handling mortality or
inadvertent disposal with fish having uninflated gas bladders. The procedure
has applications in research, production-scale fish culture, and fishery
management.
Descriptors: grading, swim bladder, anaesthetics,
abnormalities, Morone saxatilis, fish culture, normal vs. uninflated,
tricaine
ASFA; Copyright © 2003, FAO
Chatain B, Corrao D (1992) A
sorting method for eliminating fish larvae without functional swimbladders.
Aquaculture. 107(1):81-88
NAL Call No. SH1 A6
The authors describe a simple sorting method for
separating cultured fish larvae with functional swimbladders from those without
based on density differences. The whole population was first anaesthetized with
MS 222 and then the fish were separated: fish with a functional swimbladder
float and those without sink. The efficiency of the separation method was
tested at several anaesthetic doses (0.02 to 0.1 g/l) with sea bass (Dicentrarchus labrax) and sea bream (Sparus auratus) larvae in the 6-34 mm
(total length) range. The minimal sorting size was 15 mm for sea bass with an
optimal anaesthetic dose of 0.07 g MS 222/l. There were not enough data to draw
conclusions for sea bream. The method was satisfactory when applied in real
conditions to a large (90,000) population of sea bass fry with an efficiency
ratio of over 80%.
Descriptors: fish larvae, aquaculture techniques, fish
culture, Dicentrarchus labrax, Sparus aurata, swim bladder, separation
processes
ASFA; Copyright © 2003, FAO
NAL Call No. QP1 C6
Changes in the metabolism of high-energy phosphates after administration of an
anesthetic, phenthiazamine hydrobromide (2-amino-4-phenylthiazole; APT), were
studied in the loach (Cobitis biwae)
by in vivo 31P nuclear magnetic resonance (31P -NMR).
Anesthetic effects appeared at about 7 min after the loach was placed in 50 ppm
APT solution. Coincident increase in phosphocreatine (PCr) and a decrease in
inorganic phosphate (Pi) were observed. PCr returned to the preanesthetic level
when the anesthetic solution was replaced with fresh water. beta
-ATP was almost unchanged during APT anesthesia.
Descriptors: fish physiology, animal metabolism, ATP,
anaesthesia, Cobitis biwae
ASFA; Copyright © 2003, FAO
Cho G, Heath D (2000) Comparison
of tricaine methanesulphonate (MS222) and clove oil anaesthesia effects on the
physiology of juvenile chinook salmon Oncorhynchus tshawytscha
(Walbaum). Aquaculture Research. 31(6):537-546
NAL Call No. SH1 F8
This study investigated the feasibility of using clove
oil as an alternative to tricaine methanesulphonate as a fish anaesthetic,
particularly in fish stress research. The physiological stress responses of
juvenile chinook salmon Oncorhynchus
tshawytscha (Walbaum) anaesthetized with either tricaine (50 mg/L or clove oil (20 p.p.m.) were compared using
unanaesthetized fish as controls. Haematocrit, serum cortisol and serum glucose
concentrations, serum lysozyme activity and differential leucocyte counts were
measured from blood samples collected before, during and upon recovery from
anaesthesia and at specified intervals up to 72 h after recovery. Differences
between the two anaesthetic groups were not significant for most of the
physiological traits measured. Serum lysozyme activity of control fish,
however, was significantly suppressed relative to the treated fish for 72 h
after stress. Clove oil may be a safe and cost-effective alternative to tricaine
without significantly affecting study results. Furthermore, clove oil may be
more practical for field-based research, because a withdrawal period is
unnecessary, and clove oil does not pose an environmental hazard.
Descriptors: fish culture, anaesthesia, aquaculture
techniques, biological stress, fish physiology, Oncorhynchus tshawytscha,
Chinook salmon
ASFA; Copyright © 2003, FAO
Cho YJ, Cho MS, Kim SM, Choi YJ
(1997) Effect of anesthesia killing and non-bleeding on physicochemical
properties of plaice Paralichthys olivaceus muscle at early period after
death. Journal of the Korean Fisheries Society.
ISSN: 0374-8111
This study was performed to clarify the effect of
anesthesia killing and non-bleeding on the physicochemical and rheologicai
properties of plaice Paralichthys
olivaceus muscle at early period after death. Live plaice was killed by 2
different methods: spiking at the brain instantly with bleeding or dipping in
seawater containing anesthetic (2,000 ppm ethyl-aminobenzoate) for 10 min
without bleeding. These samples were stored at 0°C and used in checking
rigor-mortis, ATP breakdown, the content of ATP and its compounds, breaking
strength, and lactate accumulation through storage. The rigor-mortis ATP
breakdown, and lactate accumulation was faster in samples killed by spiking
than in anesthesia. ATP in samples killed by anesthetic showed little breakdown
until 22.5 hrs, but it was decomposed completely after 30 hrs storage. Breaking
strength of samples killed by spiking at the brain instantly with bleeding
decreased steadily and showed the maximum value over 10 hrs (2207.3 plus or
minus 60.2 g). However, in the case of the dipping fresh flesh without bleeding
in seawater containing anesthetic, the value and time reached around the
maximum breaking strength were 2147.8 plus or minus 29.0 g and 13 hrs
respectively, but it maintained constantly until 20 hrs passed. From these
results it could be suggested that anesthesia killing and non-bleeding is more
effective in maintaining firmness of fresh plaice muscle than spiking killing
with bleeding at the early period after death.
Descriptors: food fish, anaesthesia, physicochemical
properties, muscles, rheology, quality assurance, Paralichthys olivaceus,
spiking, bleeding
ASFA; Copyright © 2003, FAO
Chung KS (1980) Cold anaesthesia of tropical fish.
Bulletin of the Japanese Society for Scientific Fisheries. 46(3):391
NAL Call No.
414.9 J274
Descriptors: fish physiology, temperature tolerance,
anaesthesia, tropical fish, cold anaesthesia.
Clark KJ (2000) Temperature and
species comparisons of benzocaine pharmacokinetics, metabolism and
physiologically based pharmacokinetic model within channel catfish, Ictalurus
punctatus, and Yellow Perch, Perca flavescens. Dissertation
Abstracts International Part B: Science and Engineering. 60(8):3880
NAL Call No. Film S-1806
Benzocaine, a local anesthetic for mammals, has
potential for use as a general anesthetic in finfish. Its pharmacokinetics,
metabolism, and residue profiles were characterized to support its approval in
fish. To compare interspecies and temperature differences of these parameters
channel catfish were studied at 16°C, 21°C, and 26°C and yellow perch at 16°C.
The feasibility of "crop grouping" was investigated with a physiologically
based pharmacokinetic (PBPK) model as a means to reduce the amount of testing
required for aquaculture drug registration. Plasma concentration-time profiles
and residue depletion profiles of benzocaine in fish tissues and fluids were
characterized after fish were administered an intraarterial constant-rate
infusion of 60.5 mu mol/kg benzocaine for 30 minutes. The tissue partition
coefficients for benzocaine and its main metabolites were determined by fish
exposed to 18.4 mu mol/L benzocaine bath for 24 hours. Benzocaine,
acetylbenzocaine, p-aminobenzoic acid, and acetyl-p-aminobenzoic acid
concentrations were determined by reverse isotope dilution using HPLC and LS
counting. The PBPK model was comprised of seven tissue groups connected by a parallel
circulatory system, in which elimination was by liver and trunk kidney
metabolism, and branchial excretion. Benzocaine disappearance from plasma
conformed to a two-compartment model. Comparison between benzocaine metabolic
clearances and total body clearances implied that blood flow across the gills,
not metabolism, was its primary route of elimination. By 48 hours, less than
2.1% of the dose remained in the fish, of which a small percentage was in the
white muscle (edible tissue). The benzocaine blood-to-water concentration ratio
was 2.17-2.80 for catfish and 5.47 for perch. The magnitude of the tissue
partition coefficients suggested that tissues would not retain benzocaine if
elimination were perfusion rate limited. Benzocaine was 63-76% nonsaturable
bound to plasma proteins. In fact the combined low affinity, high capacity and
high affinity, low capacity plasma protein binding accounted for the rapid
initial elimination of benzocaine followed by a longer terminal elimination.
The excellent correspondence between experimental and simulated data suggested
that the PBPK model was representative of the “true” physiology of fish with
regard to benzocaine exposure.
Descriptors: fish culture, anaesthesia, toxicity tests,
biodegradation, fish physiology, chemical kinetics, temperature effects,
bioaccumulation, Ictalurus punctatus, Perca flavescens, channel catfish,
graceful catfish, yellow perch
ASFA; Copyright © 2003, FAO
Cooper AR, Morris S (1998) The blood respiratory, haematological, acid-base
and ionic status of the Port
NAL Call No. QP1 C6
The effects of caudal cannulation on the blood physiology of the Port Jackson
shark, Heterodontus portusjacksoni,
were investigated in sharks given between 4 and 72 h to recover from surgery.
Neither the PaO2-Pv O2 difference nor the CaO2-CvO2
difference of cannulated sharks fluctuated throughout the sampling period. The
plasma acidosis exhibited 4 h after surgery was partially compensated after 24
h by a respiratory (hyperventilatory) alkalosis and after 72 h by a marked
metabolic alkalosis. Whilst H.
portusjacksoni exhibited some cell swelling after surgery the
haematological status of cannulated sharks generally varied little throughout
the recovery period. In contrast, marked changes in plasma and erythrocyte ion
concentrations were indicative of increased branchial and erythrocyte ion
permeability. The blood status of H.
portusjacksoni given 72 h to recover from surgery was also compared with
sharks sampled by caudal puncture. The respiratory and acid-base status of
sharks sampled by caudal puncture was comparable to that of cannulated sharks.
In contrast, the plasma ion concentrations of the cannulated sharks were
markedly elevated and the erythrocyte ion concentrations concomitantly reduced
when compared with punctured sharks. The apparent increase in the water and ion
permeability of cannulated sharks was reflected by the reduced [Hb] and mean
cell haemoglobin concentrations (MCHC). Blood sampling by caudal puncture
appeared to reduce the haematological and ionic perturbations that resulted
from surgery and thus provided a less invasive and reliable method for
obtaining samples from ‘non-disturbed’ elasmobranchs.
Descriptors: fish physiology, anaesthesia, haematology, pH
effects, biological stress, Heterodontus portusjacksoni, Port Jackson
shark
ASFA; Copyright © 2003, FAO
Cubero L, Molinero A (1997) Handling,
confinement and anaesthetic exposure induces changes in the blood and tissue
immune characteristics of gilthead sea bream. Diseases of Aquatic
Organisms. 31(2):89-94
ISSN: 0177-5103
The gilthead sea bream Sparus aurata is a species of great interest for aquaculture, and
in the last few years its culture has increased in the
Descriptors: fish culture, fish handling, anaesthetics,
fish physiology, haematology, leukocytes, immunity, tissues, Sparus aurata
ASFA; Copyright © 2003, FAO
NAL Call No. SH1 A6
An effective anaesthetic for oysters was sought, in
order to allow for experimental manipulation of live oysters (Ostrea edulis) with minimal stress. A
range of anaesthetic commonly used with gastropods and cephalopods, as well as
a fish anaesthetic, were tested. Most of the chemicals tested were found to be
unsuitable for oysters. Magnesium chloride was the most successful agent,
inducing anaesthesia quickly, allowing rapid recovery with minimal stress and
mortality.
Descriptors: anaesthesia, drugs, Ostrea edulis, oyster
culture, pharmacology
ASFA; Copyright © 2003, FAO
Davidson GW, Davie PS, Young G,
Fowler RT (2000) Physiological responses of rainbow trout Oncorhynchus
mykiss to crowding and anesthesia with AQUI-S super(TM). Journal of
the World Aquaculture Society. 31(1):105-114
NAL Call No. SH138 W62
Following exposure to the anesthetic AQUI-S super(TM),
plasma cortisol concentration in immature rainbow trout was measured as (mean)
293 plus or minus 48 ng/mL, which was significantly (P > 0.05) higher than
the mean concentration in resting fish. Cortisol concentrations remained
significantly (P > 0.05) elevated for at least 24 h after treatment. This
was accompanied by a significant increase and decrease in hematocrit and plasma
potassium, respectively. These perturbations continued for at least 48 h
following recovery from anesthesia. Plasma concentrations of total protein and
sodium remained unchanged following anesthesia with AQUI-S super(TM). Crowding
stress is commonly encountered by fish during manipulation in aquaculture
situations. Anesthetising fish prior to, and during, manipulation may reduce
the associated stress. Changes in cortisol values resulting from crowding (30
min; 0.1 kg/L) during anesthesia with AQUI-S super(TM) were not appreciably
different from those in fish crowded without anesthesia. Thus, anesthesia with
AQUI-S super(TM) at the recommended dose of 17 mg/L did not appear to be
effective for alleviating the stress of crowding under the conditions of our
experiments.
Descriptors: fish culture, anaesthesia, fish physiology,
biological stress, hormones, Oncorhynchus mykiss, rainbow trout
ASFA;
Copyright © 2003, FAO
de Carvalho Gomes L (2001) Effect
of temperature on the safest level of benzocaine as an anesthetic for juveniles
of Colossoma macropomum. Aquaculture 2001: Book of Abstracts. p. 251.
Benzocaine is widely used and less expensive anesthetic for fish in
Descriptors: temperature effects, anaesthetics,
aquaculture techniques, Colossoma macropomum,
ASFA; Copyright © 2003, FAO
de Carvalho Gomes L, Roubach R,
Araujo-Lima CARM (2002) Effect of density on survival and water quality for
transporting Colossoma macropomum juveniles with CO2 as an
anesthetic. Aquaculture 2001: Book of Abstracts. p. 252
Transportation of live fish is an essential procedure for fish culture
and management. Previous studies demonstrated that the mortality of Colossoma macropomum juveniles decreased
with the addition of CO2 (50 mg/L) as an anesthetic during
transportation. The aim of this paper is to verify the effect of high density
on mortality and water quality during transportation with CO2.
Juveniles were stocked in the bags in different density (fishes/L) i.e. 30,
60, 80 and 100. CO2 was added to the water by bubbling the
gas. These bags were maintained for 24 h and mortality and physico-chemical
parameters of water were monitored. A significant correlation between density
and mortality was observed, increased the mortality with density. Transportation
of C. macropomum without anesthetics
showed a mortality rate of 10-30%. Transportation with the addition of CO2
exhibited a negligible mortality at 30 fishes/L. However, 60 and 100% of mortality
were recorded at 80 and 100 fishes/L. Concentrations of CO2
and O2 in the water with high densities were respectively
high and low with the lethal range for many fish species. However no significant
relation could be noticed between these parameters and the mortality rate.
In spite of correlation not being observed in isolated parameters the combination
of the high CO2 and low O2 concentrations
is the most probable explanation for the mortality of fish transported in
high densities. Therefore, the addition of CO2 as an anesthetic
in low densities (30 fishes/L) for long transportation is recommended.
Descriptors: water quality, aquaculture techniques,
anaesthetics, carbon dioxide, dissolved oxygen, transportation, population
density, survival, mortality causes, Colossoma macropomum, Brazil,
juveniles
ASFA; Copyright © 2003, FAO
de M C Gontijo AM, Barreto RE, Volpato GL,
Reyes VAV, Salvadori DMF (2001) Why not
anesthetize fish? In vivo and ex vivo evidence of no interference of benzocaine
in the comet assay. Mutation Research. 483(Supplement 1):S171
NAL
Call No. QH431.M8
Descriptors: animal welfare, biomonitoring, fish, benzocaine,
local anesthetic, alkaline comet assay, methods, blood
Deacon N, White H, Hecht T (1997) Isolation
of the effective concentration of 2-phenoxyethanol for anaesthesia in the
spotted grunter, Pomadasys commersonnii, and its effect on growth. Aquarium
Sciences and Conservation. 1(1):19-27
ISSN: 1357-5325
2-Phenoxyethanol is a highly suitable anaesthetic for use with fish. This paper
describes the isolation of an effective concentration of this anaesthetic for
use with the spotted grunter, Pomadasys
commersonnii. Routine anaesthesia with 2-phenoxyethanol was found to have
no significant effect on the growth of this fish.
Descriptors: aquaculture techniques, fish culture, marine
aquaculture, anaesthetics, Pomadasys commersonnii, growth
ASFA; Copyright © 2003, FAO
Derozier C (1989) Phenoxy-2
Ethanol anaesthesia in fish. Thesis, Ecole
Nationale Veterinaire,
The author studies the anaesthetic
effects of phenoxy-2 ethanol in comparison with other anaesthetics utilized in
aquaculture. Phenoxy-2 ethanol does not offer great advantages except for
better safety, but is less expensive. Further studies must determine whether
the flesh of anaesthetized fish retain phenoxyl-2 ethanol residues liable to be
noxious to human health.
Descriptors: anaesthesia, toxicants, toxicity tests,
aquaculture techniques, fish culture, Pisces, phenoxy-2 ethanol
ASFA; Copyright © 2003, FAO
Dick G (1973) Some
observations on the use of MS 222 Sandoz trial I. Scientific Report,
Research and Development Department, White Fish Authority (
(1) The fish are much easier caught and handled when
tranquillised, they are less active and are liable to do themselves less damage
in this state. (2) The fish would be less liable to stress by disturbances such
as might be incurred in transportation, bumping etc., if the fish were
tranquillised (3) The use of MS222 Sandoz at low concentrations of 1:75,000 and
1:100,000 would be sufficient to slow the fish down to enable catching and also
to keep them tranquil during transportation for a duration of 48 hours. (4)
There seems little observable difference in the effect of 1:75,000 and 1:100,
000 on the fish over 48 hours, 1:100, 000 would be cheaper. (5) To use high
concentrations of 1:10,000 and 1:30,000 would be dangerous if dealing with
large numbers of fish. The operation would have to be carried out swiftly as
prolonged immersion has been found to be lethal after 45 minute and 9 hours
respectively. At these concentration the fish would
become completely anaesthetised. (6) It is felt that at a concentration of
1:50,000 over 48 hours may well have produced unmeasurable internal damage.
After 22 hours, the fish seem to be fighting against the anaesthetic and kept
this up for the duration of time.
Descriptors: MS222 Sandoz, anesthesia, stress,
concentration, dosage
ASFA; Copyright © 2003, FAO
Durve VS (1975) Anaesthetics in
the transport of mullet seed. Aquaculture. 5(1):53-63
NAL Call No. SH1 A6
The author reports the results of an investigation on
the use of anaesthetics in the transportation of live mullet seed. Experiments
were carried out on 13 anaesthetics to ascertain the dosages suitable for
transportation of live fish. These doses were further tested for the tolerance
of mullet fry and fingerlings for a maximum period of 2 hr. Metabolism
experiments were performed to find out the degree of decrease in the metabolism
in terms of active O2 consumption. Finally, results are given
of the trial consignment despatched by rail for a transport period of 20 hr.
The results indicate that out of the 13 anaesthetics tried, 7 were suitable for
transportation of live fish. The relative merits and demerits of these
anaesthetics are further discussed.
Descriptors: anaesthesia, fish culture, Mugilidae
ASFA; Copyright © 2003, FAO
Edwards S, Burke C, Hindrum S,
Johns D (2000) Recovery and growth effects of anaesthetic and mechanical
removal on greenlip (Haliotis laevigata) and blacklip (Haliotis rubra)
abalone. Journal of Shellfish Research. 19(1):510
NAL Call No. SH365 A1 J6
Haliotis
laevigata (39.7 plus or minus 0.2 mm,
8.2 plus or minus 0.1 g) and Halitos
rubra (41.9 plus or minus 0.1 mm, 11.3 plus or minus 0.1 g) were acclimatised
to conditions over 3-5 weeks (80 animals per 80 cm diameter fibreglass tank,
flow-through sand-filtered seawater 17°C, artificial diet adlib). Animals were
then removed from the tanks using ethanol (3%), 2-phenoxyethanol (1 mL/L),
benzocaine (100 ppm), clove oil (0.5-1.5 mL/L) and mechanical removal (metal
spatula), measured, and returned to clean water for a further six weeks. At
treatment, a sample of the animals was transferred to a multichannel
flow-through respirometer for analysis of oxygen uptake, which lasted at least
3 days. All treatments were duplicated for both species. One set (control)
remained undisturbed from the beginning of the acclimation period to the end of
the trial. Additional respirometry trials were conducted on the same cohorts
for KCl (10 g/L), Aqui-S (50 ppm) and
Descriptors: fish diseases, mollusc culture, anaesthesia,
disease control, Haliotis laevigata, Haliotis rubra
ASFA; Copyright © 2003, FAO
Erdmann MV (1999) Clove oil: an
'eco-friendly' alternative to cyanide use in the live reef fish industry? Live
Reef Fish Information Buletin. 5:4-7
ISSN: 1026-2040
The use of clove oil in aquarium trade has been a new
idea in
Descriptors: ornamental fish, stupefying methods, fish
poisoning, trade, cyanides, live storage, transportation, aquaria, clove oil
ASFA; Copyright © 2003, FAO
Etienne T (1989) Fish
anaesthesia. Thesis, Ecole Nationale Veterinaire,
After a brief review of physiological and anatomical particularities of fish,
the author presents the various methods of chemical and physical anaesthesia:
(anaesthetics, electronarcosis, hypothermia).
Descriptors: anaesthesia, aquaculture techniques, fish
culture, fish physiology, literature reviews, Pisces
ASFA; Copyright © 2003, FAO
Falls WW, Vermeer GK, Dennis CW (1988)
Evaluation of etomidate as an anesthetic for red drum, Sciaenops ocellatus.
Red Drum Aquaculture. Proceedings of aSymposium on the Culture of Red Drum
and Other Warm Water Fishes.Contributions in Marine Science. 30 (suppl):37-42
NAL Call No. 442.9 T31
Etomidate was evaluated as an anesthetic in fourteen
female and seven male red drum. Fish were anesthetized every 2 or 3 weeks with
etomidate dosage levels of 0.8, 1.6, or 8.0 mg/L during a pre-spawning conditioning
regime. The 8 mg/L dosage was found to be too high as fish reached stage 4
anesthesia (loss of reflex activity) in less than 30 sec. At a 0.8 mg/L dosage,
mean induction and recovery times were excessively long. Further trials at
these 2 dosages were discontinued. Between the induction and recovery periods,
fish were maintained in a respirator at a 0.4 mg etomidate/L dosage for a mean
time of 17.47 min. An induction dose of 1.6 mg/L and a respirator maintenance
dose of 0.4 mg/L appeared suitable for routine fish handling.
Descriptors: anaesthetics, fish handling, Sciaenops
ocellatus, aquaculture techniques
ASFA; Copyright © 2003, FAO
Farwell CJ (1978) The use of
the fish anesthetic quinaldine at Scripps aquarium-museum. Annual Proceedings of the American
Association of Zoologists and Parks Aquarists. 61-71
The use of an anaesthetic such as quinaldine when removing fish from a display
tank not only simplifies the job, but helps in lowering the chances of damaging
and stressing the fish that can lead to future health problems. The amount of
quinaldine needed is determined by tank volume and the final concentration
required and the stages of anaesthesia observed in the fish are first
irritation or hyperactivity, which is quickly followed by a loss and then a
total loss of equilibrium. At this point a high respiratory rate that is weak
and interrupted by gasps is apparent. The fish are relatively still and can be
handled without struggling. The fish recover quickly from the effects.
Descriptors: aquaria, ornamental fish, anaesthetics, fish
handling
ASFA; Copyright © 2003, FAO
Ferck BJ, Jameson JD, Ramadhas V
(1996) Carbonic acid as an anaesthetic in the transport of fishes.
Water Quality Issues in Aquaculture. Proceedings of the National Seminar
on Water Quality Issues in Aquaculture Systems, Dec. 18 and 19, 1996.
pp. 103-108.
Fish seed and broodfish usable in fish farming or for sale need to be
transported in live condition which may involve considerable time and distance.
In this connection, their packing with anaesthetics is indispensable. The
present investigation was undertaken to determine the safer limit of carbonic
acid as an aneasthetic for the the transport of fry of 4 species of ornamental
fishes viz. Gymnocorymbus ternetzi, Mollienesia latipinna, Carassius auratus and Xiphophorus helleri. The fish fry of
these species were sedated with carbonic acid in 50, 100, 150 and 200 ppm
concentrations with and without oxygen. Periods for indiction, recovery and
death were recorded simultaneously for all the species. An extremely low
induction time of 13.3 seconds at 200 ppm carbonic acid was recorded for G. ternetzi and M. latipinna and a high recovery time of 187-195 seconds of X. helleri and M. latipinna, C. auratus
and G. ternetzi exhibited 80-100%
survival in all the concentration of carbonic acid. The technology of packing
commercially important fishes using the above anaesthetic is also discussed in
detail.
Descriptors: ornamental fish, anaesthetics, carbonic acid,
Gymnocorymbus ternetzi, Mollienesia latipinna, Carassius auratus, Xiphophorus
helleri, transportation, oxygen consumption, survival, green swordtail
ASFA; Copyright © 2003, FAO
Ferreira JT, Schoonbee HJ, Smit GL
(1984) The anaesthetic potency of benzocaine-hydrochloride in three freshwater
fish species. South African journal of Zoology = Suid-Afrikaanse Tydskrif
vir Dierkunde. 19(1):46-50
NAL Call No. QL336 Z6
Anaesthesia was induced in the common
carp, Cyprinus carpio, tilapia, Oreochromis mossambicus and rainbow
trout, Salmo gairdneri, at
concentrations of 25; 50; 75 and 100 mg/l of benzocaine-hydrochloride as well
as neutralized benzocaine-hydrochloride at water temperatures of 15o;
20o and 25o. The results obtained indicated intra- and
interspecific differences in the susceptibility of fish to anaesthesia due to
metabolic, chemoreceptive and temperature tolerance differences in and amongst
the three species.
Descriptors: anesthetics, performance assessment, Cyprinus
carpio, Oreochromis mossambicus, Salmo gairdneri, benzocaine-hydrochloride
ASFA; Copyright © 2003, FAO
Ferreira JT, Schoonbee HJ, Smit GL
(1984) The use of benzocaine-hydrochloride as an aid in the transport of
fish. Aquaculture. 42(2):169-174
NAL Call No. SHI A6
The potential of benzocaine-hydrochloride as an aid in
the transport of fish was investigated. When used at a concentration of 25 mg/l
the anaesthetic caused a reduction in the excretion of ammonia and carbon
dioxide by the fish, while, as a result of the reduced activities of the fish,
the pH and alkalinity values of the transport water remained fairly constant.
When benzocaine-hydrochloride was not used, the activities of the fish produced
a significant deterioration in water quality evidenced by the accumulation of
ammonia and carbon dioxide.
Descriptors: fish handling, transportation, anesthetics,
aquaculture, ethyl aminobenzoate hydrochloride, Pisces,
benzocaine-hydrochloride
ASFA; Copyright © 2003, FAO
Ferreira JT, Schoonbee HJ, Smit L
(1984) The uptake of the anaesthetic benzocaine hydrochloride by the gills
and the skin of three freshwater fish species. Journal of Fish Biology. 25(1):35-41
NAL Call No. QL614 J68
The uptake of benzocaine hydrochloride and neutralized benzocaine hydrochloride
by the skin and the gills of Cyprinus
carpio, Oreochromis mossambicus
and Salmo gairdneri were studied. The
differences observed can mainly be ascribed to degree of ionization and the
lipid solubility of the anaesthetic.
Descriptors: anesthetics, gills, skin, pharmacology, Cyprinus
carpio, Oreochromis mossambicus, Salmo gairdneri, benzocaine hydrochloride
ASFA; Copyright © 2003, FAO
Ferreira JT, Smit GL, Schoonbee HJ
(1981) Haematological evaluation of the anaesthetic benzocaine hydrochloride
in the freshwater fish Cyprinus carpio L. Journal of Fish
Biology. 18(3):291-297
NAL Call No.
QL614 J68
The effects of anaesthetization with different concentrations of benzocaine
hydrochloride (BH) and neutralized benzocaine hydrochloride (NBH) were studied
on the haematology of C. carpio. Due
to its acidic nature and resultant effects on aquarium water, BH produces
haemoconcentration effects with disturbances in acid-base function. The use of
NBH, whereby water quality effects were drastically reduced, improved the general
haematological profile. In contrast, haemodilution resulted when blood was
sampled without the use of an anaesthetic agent.
Descriptors: anaesthetics, haematology, Cyprinus carpio,
Cyprinidae, Pisces
ASFA; Copyright © 2003, FAO
Ferreira JT, Smit GL, Schoonbee
HJ, Holzapfel CW (1979) Comparison of anesthetic potency of benzocaine
hydrochloride and MS-222 in two freshwater fish species. Progressive
Fish Culturist. 41(3):161-163
NAL Call No. 157.5 P94
The hydrochloride of ethyl p-aminobenzoate was synthesized
and its anesthetic potency compared with that of MS-222 at concentrations of
50, 80, and 100 mg/l. The free compound of these agents in fish blood was also
determined. The results indicate that benzocaine hydrochloride is a more
effective anesthetic than MS-222 at the concentrations applied. Benzocaine
hydrochloride is not registered for fishery use in the
Descriptors: anaesthetics, freshwater fish, Cyprinus
carpio, Sarotherodon mossambicus, Cyprinidae, Pisces, Cichlidae
ASFA; Copyright © 2003, FAO
Gerwick L, Demers NE, Bayne CJ
(1999) Modulation of stress hormones in rainbow trout by means of
anesthesia, sensory deprivation and receptor blockade. Comparative
Biochemistry and Physiology, A. 124A(3):329-334
NAL Call No. QP1 C6
Sympathetic activation leading to increased levels of blood catecholamines, and
stimulation of the hypothalamic-pituitary-inter-renal axis leading to increased
cortisol, are difficult to avoid when handling animals. Yet, in research on
effects of acute stress, elicitation of such responses must be minimized in the
control groups. The work examines means to achieve a minimally disturbed state
in rainbow trout (Oncorhynchus mykiss).
Level of arousal was determined by adrenaline and cortisol concentrations in plasma,
and by the spleen:somatic index. Fish were prepared for bleeding by rapid
capture and concussion, by infusion of anesthetic into the undisturbed home
tank, by confinement in black boxes, or by being fed alpha - and beta -receptor
antagonists. Even when done quickly, netting and concussion yielded fish with
ca. 200-pmol adrenaline/ml plasma. Cortisol was elevated (to > 10 ng/ml)
within 30 s of stress initiation. Surreptitious infusion of anesthetic
(2-phenoxyethanol, PE) into tanks yielded fish with lower adrenaline levels
(means 19.34 and 19.58 pmols/ml in home tank and black boxes, respectively).
Among fish given phentolamine and propranolol, spleen:somatic indices and
plasma adrenaline were higher than in diet controls, whether undisturbed or stressed,
indicative of successful receptor blockade. Since careful infusion of 2-PE
yielded the lowest adrenaline levels, and requires no special apparatus, it is
the method of choice for obtaining minimally stressed fish.
Descriptors: biological stress, fish handling, hormones,
anaesthetics, therapy, fish culture, drugs, serological studies, endocrinology,
aquaculture techniques, anaesthesia, Oncorhynchus mykiss, rainbow trout
ASFA; Copyright © 2003, FAO
Ghion F (1975) First promising
results in the use of Fluothane as general anesthetic for fish. Italian
Review of Fish Culture and Fish Pathology. 10(4):111-112
NAL Call No.
SH1 R5
Fluothane (2-bromo-2-chloro-1, 1, 1, -trifluoro-ethane) a general anesthetic
for humans, has been tested on euryhaline fish (Dicentrarchus labrax, Sparus
auratus, Mugil cephalus) with
promising results. The anaesthetic is bubbled into the water mixed with air and
its delivery is stopped when fish start to swim erratically. Narcosis was
always attained within 10 min from the beginning of anaesthesia. Fish recovery
rapidly occurred when pure air was bubbled into the aquarium. Fish were safely
exposed to the anaesthetic for times ranging up to 8 h. Gross evaluation of
Fluothane concs inducing immediate narcosis gave values {approx} 40 ppm.
Descriptors: anaesthesia, Dicentrarchus labrax, Sparus
aurata, Mugil cephalus
ASFA; Copyright © 2003, FAO
Gilderhus PA (1990) Benzocaine
as a fish anesthetic: Efficacy and safety for spawning-phase salmon. Progressive
Fish Culturist. 52(3):189-191
NAL Call No. 157.5 P94
The anesthetic benzocaine was tested for efficacy and safety for spawning-phase
chinook salmon (Oncorhynchus tshawytscha)
and Atlantic salmon (Salmo salar) at
federal fish hatcheries. Benzocaine concentrations of 25-30 mg/L anesthetized
most fish in less than 3.5 min, and most fish recovered in less than 10 min
after 15 min of exposure. Safety margins were narrow; both species tolerated 30
mg/L for about 20 min, but 25 min of exposure caused deaths. For 15-min
exposures, concentrations of 35 mg/L for chinook salmon and 40 mg/L for
Atlantic salmon were lethal.
Descriptors: fish culture,
hatcheries, anaesthetics, toxicity tolerance, Oncorhynchus tshawytscha, Salmo
salar, spawning, benzocaine
ASFA; Copyright © 2003, FAO
Gilderhus PA,
NAL Call No. 157.5 P94
Benzocaine was tested as an anesthetic on juvenile and mature adult striped
bass (Morone saxatilis). Concentrations
of 55 mg/L at 22°C to 80 mg/L at 11°C effectively anesthetized fish in about 3
min. Recovery was more rapid as temperature increased. Fish survived
concentrations of twice the effective concentration and exposure times up to 60
min at the effective concentration. Striped bass required higher concentrations
for anesthetization than had been previously demonstrated for salmonid fishes,
but safety margins for both concentration and exposure time were wider than for
the salmonids.
Descriptors: anaesthetics, efficiency, recovery,
temperature effects, Morone saxatilis, fish culture, benzocaine
ASFA; Copyright © 2003, FAO
Gingerich WH, Drottar KR (1989) Plasma
catecholamine concentrations in rainbow trout (Salmo gairdneri) at rest
and after anesthesia and surgery. General and Comparative Endocrinology.
73(3):390-397
NAL Call No. 444.8 G28
The effects of surgery and anesthesia on
concentrations of plasma epinephrine (E), norepinephrine (NE), and dopamine
(DA) were investigated in rainbow trout (Salmo
gairdneri) fitted with dorsal aorta cannulae. Baseline catecholamines (CA)
concentrations, established in resting rainbow trout, were 1.55 plus or minus
0.90 rho mol/ml (X plus or minus SD) for E, 2.07 plus or minus 1.26 for NE, and
1.33 plus or minus 0.87 for DA. After surgery, plasma concentrations of all CAs
fell rapidly but values were still higher than baseline 6 hr after surgery,
then were near baseline at 24 and 48 hr after surgery. Plasma E and NE
concentrations in the fish during early anesthesia (1.14 plus or minus 0.14
min) were not significantly different from preanesthesia values.
Descriptors: blood, hormones, anaesthesia, biological
stress, steroids, fish physiology, Salmo gairdneri, serological studies
ASFA; Copyright © 2003, FAO
Gleadall IG (1991) Comparison
of anaesthetics for octopuses. Bulletin of Marine Science. 49(1-2):663
ISSN: 0007-4977
This study, comparing eleven different immersion anaesthetics for octopuses,
was initiated in view of the failure of conventional ethanol or urethane
anaesthesia of octopuses at lower temperatures (< 12°C), 2) a requirement
for better fine control over anaesthesia, and, a reluctance to use magnesium
chloride anaesthesis for certain experiments. A standard procedure was adopted
to compare the three well established anaesthetics (ethanol, urethane,
magnesium chloride) with several well known fish anaesthetics (tricaine,
metomidate, propoxate) and those commonly employed with invertebrates (chloral
hydrate, chloretone, menthol, nicotine sulphate, phenoxetol). Also, two methods
of anaesthesia by cooling are briefly compared. The species used were mostly Octopus vulgaris and O. fang-siao (but also included O. dofleini and Octopus sp., with doses carefully adjusted for body weight; three
different doses were used within the recommended range for each anaesthetic.
Most "invertebrate" and "fish" anaesthetics either failed
to anaesthetize the octopuses (at low doses) or they proved toxic or fatal (at
higher doses), with no intermediate anaesthetic effect. Effective anaesthetics
are used for different types of experimentary, but the search continues for a
more finely controllable, all-round anaesthetic for octopuses.
Descriptors: temperature effects, Octopoda, comparative
studies, anaesthetics
ASFA; Copyright © 2003, FAO
Gomes LC, Chippari-Gomes AR, Lopes
NP, Roubach R, Araujo-Lima CARM (2001) Efficacy of Benzocaine as an
Anesthetic in Juvenile Tambaqui Colossoma macropomum. Journal of
the World Aquaculture Society. 32(4):426-431
NAL Call No. SH138 W62
The present study investigated the use of benzocaine as an anesthetic for
juvenile Colossoma macropomum (tambaqui).
In the first experiment, fish were exposed to various doses of benzocaine for
10 min at 24oC. The second experiment examined the effects of duration
of exposure to 100 mg/L of benzocaine. In the third experiment, fish were
exposed to 100 mg/L at temperatures of 24oC , 27oC,
and 30oC. Benzocaine concentrations of 100-150 mg/L were considered
ideal for quickly inducing total immobilization and fast recovery. Fish exposed
to 350 mg/L benzocaine exhibited 30% mortality. No changes in hematocrit were
recorded in fish exposed to different concentrations of benzocaine. Plasma
glucose increased significantly when fish were exposed to benzocaine concentrations
greater than 200 mg/L. Recovery time after a 30-min exposure to 100 mg/L
benzocaine was significantly greater than after an exposure for 10 and 20 min.
No mortality was observed 96 h after exposure to 100 mg/L benzocaine for 10,
20, and 30 min. Dosages in the 100-150 mg/L range were effective for periods of
up to 20 min of anesthesia. There was no effect of temperature on the time
required for fish to lose equilibrium. However, recovery was significantly
faster for fish anesthetized at 30oC. Benzocaine is an effective
anesthetic agent for tambaqui juveniles, providing rapid immobilization and
rapid recovery. Benzocaine is also less expensive than other available
anesthetic compounds.
Descriptors: juveniles, experimental research, costs,
anaesthetics, Colossoma macropomum, benzocaine
ASFA; Copyright © 2003, FAO
Graham MS, Iwama GK (1990) The
physiologic effects of the anesthetic ketamine hydrochloride on two salmonid
species. Aquaculture 90(3-4):323-331
NAL Call No. SH1A6
Adult coho salmon (Oncorhynchus
kisutch) and subadult rainbow trout (Oncorhynchus
mykiss) were used in experiments with the anesthetic ketamine
hydrochloride. The drug (30 mg/kg) was injected into the dorsal aorta through
an indwelling cannula. Intravascular administration of ketamine caused an
immediate cessation of ventilation in both species for 10 s to 300 s and a loss
of balance. Ventilation rate recovered to pre-anesthesia values within 1-2 h
and arterial oxygen values were at pre-anesthesia levels by 3-24 h. Anesthesia
caused a significant acidosis in both species. The blood pH and plasma CO2
values had returned to pre-anesthesia levels by 4-24 h and 0.5-2 h,
respectively. For adult salmon, five of seven animals were unresponsive to tail
grabbing at 4 h while with juvenile trout, three of five fish were fully
responsive to touch at 1 to 2 h. This difference in duration of anesthesia was
likely size-related. The applications of this injectable anesthetic for
commercial fish use, mainly in the transport of animals, are suggested, but its
use with food fish has not been assessed.
Descriptors: anaesthetics, aquaculture techniques,
cultured organisms, transport, Oncorhynchus mykiss, Oncorhynchus kisutch,
ketamine hydrochloride
ASFA; Copyright © 2003, FAO
Guidobono F, Netti C, Sibilia V,
Villa I, Zamboni A, Pecile A (1986) Eel calcitonin binding site distribution
and antinociceptive activity in rats. Peptides 7(2):315-322
NAL Call No. QP552 P4P45
The distribution of binding sites for (125I)-eel-calcitonin
(ECT) to rat central nervous system, studied by an autoradiographic technique,
showed concentrations of binding in the diencephalon, the brain stem and the
spinal cord. Large accumulations of grains were seen in the hypothalamus, the
amygdala, in the fasciculus medialis prosencephali, in the fasciculus
longitudinalis medialis, in the ventrolateral part of the periventricular gray
matter, in the lemniscus medialis and in the raphe nuclei. In the spinal cord,
grains were scattered throughout the dorsal horns. Binding of the ligand was
displaced equally by cold ECT and by salmon CT(sCT), indicating that both
peptides bind to the same receptors. The administration of ECT into the brain
ventricles of rats dose-dependently induced a significant and long-lasting enhancement
of hot-plate latencies comparable with that obtained with sCT. The
antinociceptive activity induced by ECT is compatible with the topographical
distribution of binding sites for the peptide and is a further indication that
fish CTs are active in the mammalian brain.
Descriptors: calcitonin,
central nervous system, intracerebroventricular administration, analgesia, fish
physiology, peptides, brain, Anguilla, binding, autoradiography, distribution,
rats, neurophysiology
ASFA; Copyright © 2003, FAO
Guo FC, Teo LH, Chen TW (1995) Effects
of anaesthetics on the oxygen consumption rates of platyfish Xiphophorus
maculatus (Guenther). Aquaculture Research. 26(12):887-894
NAL Call No. SH1 F8
This study was carried out to find out the effects of anaesthetics,
2-phenoxyethanol, quinaldine sulphate, MS-222 and metomidate, at various
dosages, on the oxygen consumption rates of two size groups of platyfish, Xiphophorus maculatus (Guenther) at
three temperatures. The results show that the oxygen consumption by the
platyfish of both size groups was temperature dependent, being higher at higher
temperature, but not size dependent. The effects of anaesthetics on the oxygen
consumption rates of platyfish were dosage dependent and temperature dependent,
especially for 2-phenoxyethanol, the effect always being significantly greater
at lower temperature. Small and large fish did not show much difference in
their responses to anaesthetic treatments. However, with 2-phenoxyethanol, the
effect on the large platyfish was always better than on the small ones at
20-25°C. At 220-440 ppm and at 20°C, 2-phenoxyethanol was more effective than
the other anaesthetics in suppressing oxygen consumption by the platyfish.
Descriptors: anaesthetics, temperature effects, Xiphophorus
maculatus, oxygen consumption, ornamental fish, aquaria
ASFA; Copyright © 2003, FAO
Guo FC, Teo L-H, Chen TW (1995) Effects
of anaesthetics on the water parameters in a simulated transport experiment of
platyfish, Xiphophorus maculatus (Guenther).
Aquaculture Research. 26(4):265-271
NAL Call No. SH1 F8
An experiment was carried out to study the effects of anaesthetics
(2-phenoxyethanol, quinaldine sulphate, metomedate and MS-222) on water
parameters during simulated air transport of platyfish, Xiphophorus maculatus. The platyfish were put in sealed plastic
bags, one-quarter full of water, to which a required amount of anaesthetic was
added. The rest of the bag was filled with oxygen. The water in the bag was
tested for pH, total ammonia and carbon dioxide at intervals of 4 and 8 h for a
period of 48 h. Mortality rates within this period and the post-packaging
period were also noted. It was found that 2-phenoxyethanol was most effective,
followed by quinaldine sulphate, in decreasing the excretion of metabolic
wastes by the fish. Metomidate had no effect in the control of waste
production. MS-222 reduced ammonia excretion but not carbon dioxide. None of
the anaesthetics used had any effect on the pH of the water.
Descriptors: Xiphophorus maculatus, oxygen
consumption, anaesthetics, fish physiology, excretion
ASFA; Copyright © 2003, FAO
Hanawa M, Harris L, Graham M,
Farrell AP, Bendell-Young LI (1988) Effects of cyanide exposure on Dascyllus
aruanus, a tropical marine fish species: lethality, anaesthesia and
physiological effects. Aquarium Sciences and Conservation. 2(1):21-34
ISSN: 1357-5325
The lethality, anaesthetic and physiological effects of ‘pulsed’ cyanide (CN-)
exposures to a common tropical marine fish Dascyllus
aruanus were assessed. Cyanide (25 and 50 mg/l) was applied as pulses (10,
60 and 120 s) to fish under non-stressed and stressed (by chasing and/or
placing fish under hypoxic stress) conditions. Following treatment, the time
until recovery and the percent survival were determined. The fish were allowed
a 2.5 week recovery period from the treatments at which time four physiological
end-points were measured: (1) the blood haemoglobin content, (2) the percent
blood O2 content, (3) the liver rhodonase activity and (4) the liver
O2 consumption rate. The greater the CN- concentration
and exposure time, the longer the recovery time.
Descriptors: oxygen consumption, liver, biological stress,
cyanides, marine fish, tropical fish, fish physiology, anaesthetics, Dascyllus
aruanus, physiology, cyanide,
ASFA; Copyright © 2003, FAO
Harrell RM (1992) Stress
mitigation by use of salt and anesthetic for wild striped bass captured for
brood stock. Progressive Fish Culturist. 54(4):228-233
NAL Call No. 157.5 P94
Gravid striped bass (Morone saxatilis)
were collected from spawning grounds of the Choptank River, Maryland, to
evaluate ways of mitigating stress effects associated with capture and
transport. Stress alleviation was attempted through the use of salt (10 g/L),
anesthetic (tricaine (MS-222) at 25 mg/L), or a combination of the two. Two
capture techniques were used, electrofishing and gill netting, and all captured
fish were immediately placed in tanks with the respective treatment then
transported by truck to the hatchery. Fish stress was measured by plasma
corticosteroid and chloride levels. The times required for stressed fish to
recover, as measured by a return to baseline values of plasma corticosteroids,
indicated that stress was mitigated most effectively by salt alone. The
combination of salt and anesthetic was second in effectiveness, and the
anesthetic alone was least effective. Although fish in each treatment initially
exhibited signs of hypochloremia, only those fish transported in anesthetic
along exhibited long-term signs of hypochloremia regardless of capture method.
Descriptors: biological stress, anaesthetics, Morone saxatilis, salts, fish handling,
fish culture
ASFA; Copyright © 2003, FAO
Hattingh J (1977) The effect of
tricaine methanesulphonate (MS-222) on the microhaematocrit of fish blood. Journal
of Fish Biology. 10(5):453-455
NAL Call No.
QL614 J68
The effects of the anaesthetic, MS-222, on the microhaematocrit value of
freshwater fish have been examined. Blood containing MS-222 showed a higher
haematocrit value than blood without the anaesthetic and haemolysis occurred in
the former after a variable time depending on the concn. The results are
discussed in relation to previous findings.
Descriptors: serological studies, anaesthetics, Pisces
ASFA; Copyright © 2003, FAO
Hettiarachchi M, Senadheera SPSD
(1999) Efficacy of quinaldine sulphate as an anaesthetic for the ornamental
carp (Cyprinus carpio) in simulated packaging for long distance
transport. Sri Lanka Journal of Aquatic Sciences. 4:13-22
ISSN: 1391-2038
The high cost incurred in transporting fish in large volumes of water is a
major problem in airlifting ornamental fish to foreign markets. The present
study was carried out to investigate the efficacy of the anaesthetic,
quinaldine sulphate buffered with sodium bicarbonate on ornamental carps (Koi
carps, Cyprinus carpio) in simulated
packaging for air transport. Quinaldine sulphate significantly reduced the rate
of oxygen consumption and the accumulation of ammonia in water. The most
efficient concentration of buffered quinaldine sulphate which was responsible
for the greatest reduction in accumulation of ammonia and the rate of oxygen
consumption was 50 ppm. Young koi carps of 7.5-9.0 cm in total length
anaesthetised with 50 ppm quinaldine sulphate at the density of 40% of fish
body weight to weight of water ratio did not show any mortality at room
temperature of 28°C during the 40 hours of exposure time while unanaesthetized
fish at the same density suffered 100% mortality. The recovery time during the
post-packaging period was found to be less than 5 minutes. The present study
indicates that young koi carps could be transported at higher packing
densities, using the suitable dosage of quinaldine sulphate which will maximize
the effective utilization of space and weight during transportation.
Descriptors: anaesthetics, live storage, transportation,
ornamental fish, survival, Cyprinus carpio, common carp
ASFA; Copyright © 2003, FAO
Hignette M (1984) The use of cyanide
to catch tropical marine fish for aquariums and its diagnosis. Comptes Rendus des Journees
Aquariologiques de l'Institut Oceanographique,
ISSN: 0182-0745
Marine tropical fish for the pet industry are nowadays very often caught with
cyanide sodium, which is used for its “anaesthetic” effect. This method however
can be responsible for fish dying as much as several weeks after transport, and
must be avoided. In order to stop exporters having recourse to this practice,
fish importers and aquariologists must know how to measure cyanide themselves
in fish or obtain analyses from reliable laboratories.
Descriptors: cyanides, aquaria, tropical fish, catching
methods, fish poisoning, stupefying methods, mortality causes
ASFA; Copyright © 2003, FAO
Hirata M, Isoda S, Kanao M,
Shimizu H, Inoue S (1970) Studies on anesthetics for fish. Bulletin
of the Japanese Society for Scientific Fisheries 36(11):1127-1135
NAL Call No. 414.9 J274
92 anthranilate
derivatives were synthesized and examined for their anaesthetic effect on Carassius and puffer. Compound No. 36,
DP-1166, was the most potent anaesthetic for these fish and also showed
excellent effectiveness on other spp.
ASFA; Copyright © 2003, FAO
Descriptors: anesthetics,
DP-1166, Carassius, puffer, anthranilate derivatives
Hoffmann R, Lommel R, Riedl M
(1982) Influence of different anaethetics and bleeding methods on
hematological values in fish. Archiv fur Fischereiwissenschaft. 33(1-2):91-103
NAL Call No. SH1 A72
Erythrocyte, leucocyte and thrombocyte values,
hemoglobin, PCV and differential blood cell counts were investigated in a
cyprinid (Carassius carassius L.) and
in a salmonid (Salmo gairdneri
Descriptors: anesthetics, blood cells, hematology,
methodology, Carassius carassius, Salmo gairdneri
ASFA; Copyright © 2003, FAO
Houston AH, Corlett JT (1976) Specimen
weight and M.S. 222. Journal of the Fisheries Research Board of
NAL Call No. 442.9 C16J
The influence of specimen wt upon induction of and recovery from Stage I M.
S. 222 (ethyl m-aminobenzoate methanesulphonate) was examined in gold-fish (Carassius auratus), brook (Salvelinus fontinalis), and rainbow
trout (Salmo gairdneri) exposed to
various anesthetic concn-temp combinations. Both induction and recovery times
varied inversely with wt, the influence of wt being particularly pronounced
among smaller specimens. These observations are consistent with the hypothesis
that attainment of ‘critical’ internal anesthetic concn is influenced by
wt-specific variation in the relationship between gill area and extracellular
phase vol.
Descriptors: anaesthetics, Carassius auratus,
Salvelinus fontinalis, Oncorhynchus mykiss
ASFA; Copyright © 2003, FAO
Hovda J, Linley TJ (2000) The
potential application of hypothermia for anesthesia in adult Pacific salmon.
North American Journal of Aquaculture. 62(1):67-72
NAL Call No. SH1 N66
We subjected 190 adult pink salmon Oncorhynchus gorbuscha to water
temperatures of -1.5, -3.0°C, -4.5°C, and -6.0°C to evaluate the potential of
hypothermia for anesthesia. The temperatures were obtained by dissolving salt
(NaCl) at concentrations ranging from 25ppt to 90ppt and recirculating the
solutions through a thermostatically controlled chiller. The time to each
anesthetic stage (sluggishness, loss of movement, and complete anesthesia)
declined with decreasing temperature but did not differ significantly between
sexes. The most rapid change in response occurred between -1.5°C and -3.0°C.
Time to recovery was also influenced by temperature and was directly related to
the time to complete anesthesia. In contrast, anesthesia temperature had no
affect on egg survival, nor was there a significant difference in survival
between the experimental groups and the control (CO2). We conclude
that hypothermia is effective for short-term anesthesia of Pacific salmon Oncorhynchus spp. for spawning but note
that its application for iteroparous or freshwater stenohaline species may be
problematic because of the physiological effects induced by cold shock and
exposure to high salinity. Further work will also be needed to determine its
utility for large-scale operation.
Descriptors: temperature tolerance, hypothermia, fish
culture, survival, anaesthesia, Oncorhynchus
gorbuscha, pink salmon
ASFA; Copyright © 2003, FAO
Howe GE, Bills TD, Marking LL
(1990) Removal of benzocaine from water by filtration with activated carbon.
Progressive Fish Culturist. 52(1):32-35
NAL Call No. 157.5 P94
Benzocaine is a promising candidate for registration with the U.S. Food and
Drug Administration for use as an anesthetic in fish culture, management, and
research. A method for the removal of benzocaine from hatchery effluents could
speed registration of this drug by eliminating requirements for data on its
residues, tolerances, detoxification, and environmental hazards. Carbon
filtration effectively removes many organic compounds from water. This study
tested the effectiveness of three types of activated carbon for removing
benzocaine from water by column filtration under controlled laboratory
conditions. An adsorptive capacity was calculated for each type of activated
carbon. Filtrasorb 400 (12 x 40 mesh;
Descriptors: water filtration, anaesthetics, aquaculture
effluents, wastewater treatment, water quality control, hatcheries, biofilters,
water quality, aquiculture, activated carbon, effluent treatment, water
treatment, filtration, benzocaine, activated carbon
ASFA; Copyright © 2003, FAO
Hseu JR, Yeh SL,
NAL Call No. SH1 S48
Solutions of sodium bicarbonate (NaHCO3)
and sulphuric acid (H2SO4) were mixed in seawater to try
to anesthetize black porgy (Acanthopagrus schlegeli). The results indicated
that the anesthetic induction time was correlated positively with body size and
negatively with the concentration of NaHCO3 and H2SO4,
but only the concentration influenced the recovery time. 675 ppm NaHCO3,
mixed with 395 ppm H2SO4, might be suitable to
anesthetize black porgy because the induction time and the recovery time of most
of the fish were less than 6 minutes at this concentration. No fish died during
anesthetization and 5 days thereafter. Thus, this technique should be effective
and safe for anesthetization of black porgy. An examination was also made of
the changes of the physiological parameters of black porgy after
anesthetization by 675 ppm NaHCO3, mixed with 395 ppm H2SO4.
The values of hematocrit, hemoglobin, plasma glucose, and total plasma protein
were not significantly different between the anesthesia and the control groups.
However, the plasma chloride ion concentration decreased significantly and the
osmolarity increased significantly after anesthetization.
Descriptors: anaesthesia, sulphuric acid, fish physiology,
Acanthopagrus schlegeli, methodology, sodium bicarbonate
ASFA; Copyright © 2003, FAO
Hseu Jinn-Rong, Yeh Shinn-Lih, Chu
Yeong-Torng, Ting Yun-Yuan (1996) Influence of the anesthetic,
2-phenoxyethanol, on hematological parameters of black porgy Acanthopagrus schlegeli. Journal of
NAL Call No. SH1 S48
In this study, an examination was made of changes of hematological parameters
in black porgy Acanthopagrus schlegeli
after anesthetization in 2-phenoxyethanol (2-PE) of various concentrations. In
400 and 600 ppm 2-PE solutions, black porgies were anesthetized to total loss
of equilibrium within 3 min. All values of hematological parameters of the
anesthetized fishes were not significantly different between the anesthesia and
control groups. In 200 ppm 2-PE solution, since the fish could not be
anesthetized to total loss of equilibrium within 30 min, the black porgies
which were anesthetized for 15 min in this solution were tested and it was
found that the fish showed a significant decrease in hematocrit and increase in
plasma glucose. It is therefore concluded that higher dosages of 2-PE will take
less time to induce less stress effect on the anesthetized fish.
Descriptors: fish culture, biological stress, aquaculture
techniques, fish physiology, anaesthetics, haematology, anaesthesia, Acanthopagrus
schlegeli
ASFA; Copyright © 2003, FAO
Hseu Jinn-Rong, Yeh Shinn-Lih, Chu
Yeong-Torng, Ting Yun-Yuan (1994) The use of 2-phenoxyethanol as an
anesthetic in the transport of black porgy Acanthopagrus schlegeli. Journal of
NAL Call No. SH1 S48
A study was conducted to investigate the application
of 2-phenoxyethanol (2-PE) in a closed transport system involving black porgy (Acanthopagrus schlegeli) in polyethylene
bags. After 24 h sealed packaging, addition of 50-200 ppm 2-PE reduced the
accumulation of total ammonia-nitrogen of sea water in the bags containing
fish. However, 2-PE could not prevent acidification of the sea water in the
bags. In another experiment, effects of 2-PE on the changes of hematological
parameters in black porgy were examined at 0, 12, and 24 h after sealed
packaging. The results indicated that packaging time and anesthetic did not
affect the values of hematocrit, hemoglobin and osmolarity. The value of serum
glucose was the only changed parameter during this experiment. The values of
serum glucose increased following the duration of packaging time. The 2-PE
added group had lower average values of serum glucose than the control group,
but the differences between two groups were not significant. Considering the
effect of 2-PE on the reduction of the accumulation of total ammonia-nitrogen
in sea water and serum glucose of fish during sealed packaging as well as its
cheap price, 2-PE was recommended for application in transport of fish.
Descriptors: live storage, transportation, anaesthetics,
haematology, fish culture, Acanthopagrus schlegeli
ASFA; Copyright © 2003, FAO
Hseu Jinn-Rong, Yeh Shinn-Lih, Chu
Yeong-Torng, Ting Yun-Yuang (1994) The anesthetic effect of 2-phenoxyethanol
in goldlined sea bream (Sparus sarba). Journal
of
NAL Call No. SH1 S48
Responses of goldlined sea bream (Sparus
sarba) to the anesthetic 2-phenoxyethanol (2-PE) were investigated. The
induction time (INT) and recovery time (RET) increased when anesthesia reached
the later stages. INT increased also with lower concentration of 2-PE, while
RET showed the opposite. However, larger fish took longer INT, but RET did not
show the other way. Different intervals between two anesthesias resulted
different INT (INT2) of the second anesthesia in comparison with that of the
first INT (INTI). At 0 or 5 minutes intervals, INT2 was shorter than INT1. As
opposite to the above findings, INT2 was longer than INTI at 30 minutes, 1 hour
or 6 hours of interval. At 24 hours of interval, half of the anesthetized fish
had longer INT2 and the rest of fish had shorter INT2. The results indicated
that the anesthetic sensitivity of goldlined sea bream responding to 2-PE would
revert after 24 hour's recovery from first anesthesia.
Descriptors: anaesthetics, anaesthesia, Sparus sarba,
evaluation
ASFA; Copyright © 2003, FAO
Hseu Jinn-Rong, Yeh Shinn-Lih, Chu
Yeong-Torng, Ting Yun-Yuen (1994) The changes of hematological parameters
during sustained anaesthesia with 2-phenoxyethanol in yellowfin porgy (Acanthopagrus
latus). Journal of
Yellowfin porgy (Acanthopagrus latus)
was anaesthetized with 2-phenoxyethanol, and sampled at 0, 1, 6, 12, 24 h
during anesthesia and 24 h after recovery. Sampling time and anesthetic were
found to affect the values of hematological parameters. Most of the time, the
control group had higher average values of hematocrit and hemoglobin than the
anesthesia group, but the difference between two groups was not significant
except in hematocrit values at 24 h during anesthesia. Both sets of values of
two groups reached the least level at 12 h, and gradually recovered to starting
point. As to serum glucose, the anesthesia group had higher average values than
the control group from 1 to 12 h during anesthesia, however, the difference
between two groups was significant only at 6 h. The values of serum glucose in
the anesthesia as well as the control group decreased from starting point, and
never recovered even after 24 h recovering.
Descriptors: anaesthesia, anaesthetics, haematology, fish
physiology, Acanthopagrus latus
ASFA; Copyright © 2003, FAO
Huish MT (1972) Some responses
of the brown bullhead to MS-222. Progressive Fish Culturist. 34(1):27-32
NAL Call No.
157.5 P94
The effect of the anaesthetic MS-222 on the brown bullhead, Ictalurus nebulosus, at different temp
and conc of anaesthetic, was investigated. Deep stages of anaesthesia were produced
at 100 ppm in a relatively short time. The rate of induction of anaesthesia was
not clearly related to size of fish. Mortalities were increased and recovery
rates slowed at 22° and 27°C, as compared to 7°C, 12°C and 17°C, when treated
for 1 hr with 75 and 100 ppm. Fish exposed for 1 hr to 100 ppm at 27°C for <
12 min survived. Those exposed for > 12 min died.
ASFA; Copyright © 2003, FAO
Descriptors: MS-222, brown
bullhead, Ictalurus nebulosus, anesthesia, dosage
Imamura-Kojima H, Takashima F, Yoshida
T (1987) Absorption, distribution and excretion of 2-phenoxyethanol in
rainbow trout. Bulletin of the
Japanese Society for Scientific Fisheries. 53(8):1339-1342
NAL Call No. 414.9 J274
The absorption, distribution and excretion of 2-phenoxyethanol, which is a
piscine anesthetic, were examined in rainbow trout (Salmo gairdneri). In fish tranquilized with a sate concentration of
2-phenoxyethanol, it was distributed in the brain, liver, kidney, and gall
bladder, especially, the cerebellum. The 2-phenoxyethanol was rapidly excreted
and the biological half-life under these experimental conditions was
approximately 30 min.
Descriptors: bioaccumulation, anesthetics, excretion, Salmo
gairdneri, phenoxyethanol
ASFA; Copyright © 2003, FAO
Itazawa Y, Takeda T (1982) Respiration
of carp under anesthesia induced by mixed bubbling of carbon dioxide and
oxygen. Bulletin of the Japanese Society for Scientific Fisheries. 48(4):489-493
NAL Call No. 414.9 J274
Anesthetization of fish by mixed bubbling into the ambient water of carbon
dioxide and oxygen is being tried as a method of live transport. Respiratory
parameters were measured with carp (Cyprinus
carpio) before, during and after anesthesia induced by 1:1 mixed bubbling
of carbon dioxide and oxygen. During the anesthesia, oxygen content and oxygen
saturation of the arterial blood was maintained at levels higher than or equal
to the pre-anesthetic ones owing to very high pO2 accompanied with
elevated Ht and Hb in the blood, in spite of enormously high pO2 which
ought to reduce the oxygen affinity of the blood. Gill ventilation was also
maintained at the pre-anesthetic level due to increased frequency of
respiration, notwithstanding its reduced stroke volume. Oxygen consumption was
reduced to one-half its pre-anesthetic level, accompanied with a sharp drop of
oxygen utilization at the gills.
Descriptors: anesthesia, transportation, respiration, live
storage, Cyprinus carpio
ASFA; Copyright © 2003, FAO
Iwama GK, Mcgeer JC, Pawluk MP (1989)
The effects of five fish anaesthetics on acid - base balance, hematocrit,
blood gases, cortisol, and adrenaline in rainbow trout. Canadian Journal
of Zoology / Journal Canadien de Zoologie.
67(8):2065-2073
NAL Call No. 470 C16D
Some physiological aspects of five fish anaesthetics
in rainbow trout (Oncorhynchus mykiss)
were investigated. The effects of benzocaine, 2-phenoxyethanol, MS-222
(Sandoz), metomidate, and carbon dioxide gas (CO2) on acid - base
regulation, hematocrit, blood gases, and cortisol and adrenaline concentrations
were determined in resting rainbow trout fitted with chronic catheters in the
dorsal aorta. A severe hypoxia developed with the cessation of breathing in
deep anaesthesia. This was accompanied by a rise in blood pCO2 and
adrenaline concentration, and a fall in blood pH. Blood bicarbonate
concentrations remained unchanged and cortisol concentrations declined with
time. There was a transient increase in hematocrit coinciding with the increase
in adrenaline concentrations.
Descriptors: anesthetics, fish physiology, hematology,
steroids, hypoxia, Oncorhynchus mykiss
ASFA; Copyright © 2003, FAO
Iwama GK, Yesaki TY, Ahlborn D
(1991) The refinement of the administration of carbon dioxide gas as a fish
anesthetic: The effects of varying the water hardness and ionic content in
carbon dioxide anesthesia. ICES (International Council for the
Exploration of the Sea) Council Meeting Papers., ICES,
The results indicate that two of three treatments that involved the
addition of NaHCO3 were capable of reducing the amount of stress
experienced by juvenile steelhead (Oncorhynchus
mykiss) when anesthetized by CO2. Treatment 3, NaHCO3
only, had significantly lower cortisol, lactate, and Hct values versus the
control treatment. Consequently, it was concluded that the fish in treatment 3
exhibited the lowest overall stress responses to CO2 anesthesia. The
fish in treatment 9, NaHCO3 and NaCl, had significantly lower
cortisol and Hct values and was determined to be the next most effective
treatment in reducing the stress associated with CO2 anesthesia.
Treatments 3, 6, and 9 had water pH levels that were comparable to that of the
water in which the fish were originally held. “Hyperactivity” was observed to
be somewhat reduced when NaHCO3 was added to the anesthetic bath
water.
Descriptors: anaesthesia, carbon dioxide, biological
stress, Oncorhynchus mykiss
ASFA; Copyright © 2003, FAO
Jeney Z, Jeney G, Olah J, Siwicki
A, Danko I (1986) Propanidid, a new anaesthetic for use in fish propagation.
Aquaculture. 54(1-2):149-156
NAL Call No. SH1A6
Propanidid,
3-methoxy-4-(N,N-diethyl-carbamoyl-methoxy)-phenylacetic acid n-propyl ester,
was applied during artificial propagation of common carp, Cyprinus carpio L., and compared with MS 222. Primary and secondary
stress effects from Propanidid and MS 222 were characterized by measuring the
plasma adrenaline and noradrenaline levels, haemoglobin, blood glucose, plasma
Ca++ and Cl- concentrations, haematocrit and leucocrit
values and activity of transaminases (GOT, GPT) in plasma. General effects of
handling stress during propagation were reflected by significant hyperglycemia,
decreasing Ca++ and Cl- concentrations, and a significant
increase in transaminase activity in plasma. Both anaesthetics decreased
handling stress, though MS 222 caused a greater increase of catecholamines and
of GPT and decrease of leucocrit and plasma Ca++. Results of
artificial propagation were similar in the two experimental groups.
Descriptors: anesthetics, fish culture, biological stress,
fish handling, transportation, Cyprinus carpio
ASFA; Copyright © 2003, FAO
NAL Call No. SH219.N66
Tricaine (MS-222) is the most widely used anesthetic for fishes, but induction
and recovery times are rather long. Studies on salmonids have shown that
electroanesthesia is a good alternative to MS-222 for short term (<1 min)
immobilization. However, data on longer-duration (3-5-min) immobilization
needed for surgical procedures are lacking. We analyzed induction and recovery
times for 20 adult (52-81-cm) striped bass Morone
saxatilis immobilized with electroanesthesia and MS-222. We defined
induction time as the interval from the onset of each treatment until the fish
was immobilized (i.e., did not respond to tactile stimuli) and recovery time as
the interval from the fish's return to the water to its resumption of normal
swimming. Surgical procedures similar to those necessary to implant a radio
transmitter were performed on each fish. Induction time for fish immobilized
with electroanesthesia (geometric mean, 8 s; 95% confidence interval [CI], 3-21
s) was much shorter than that for fish immobilized with MS-222 (geometric mean,
47 s; 95% CI, 38-58 s) (P = 0.0006). Additionally, fish immobilized with
electroanesthesia recovered much faster (geometric mean, 9 s; 95% CI, 4-19 s)
than fish immobilized with MS-222 (geometric mean, 206 s; 95% CI, 156-272 s) (P
less than or equal to 0.0001). Faster induction and recovery times of fish
immobilized with electroanesthesia and the ability to process more fish per
unit time are major benefits of this technique.
Descriptors: anaesthesia, fishery
biology, tagging, fishery management, Morone saxatilis, surgery, rockfish,
mortality, striped bass
ASFA; Copyright © 2003, FAO
Josa Serrano A, Espinosa Velazouez
E, Esteban Alonso J, del Nino Jesus A, Osanz Castan E (1993) Use of anaesthetic
2-phenoxyethanol in carps (Cyprinus carpio): Levels blood concentration.
In this work we studied the 2-Phenoxy
ethanol concentration levels in the blood of Carp (Cyprinus carpio) after its use as anesthesic at different time,
concentrations, temperatures and exposition. Anaesthesia levels as well as
induction and recovery times are defined.
Descriptors: fish culture, anaesthesia, serological
studies, blood, temperature, Cyprinus carpio, 2-phenoxiethanol
ASFA; Copyright © 2003, FAO
Kahl MD, Jensen KM, Korte JJ, Ankley
GT (2001) Effects of handling on endocrinology and reproductive performance
of the fathead minnow. Journal of Fish Biology. 59(3):515-523
NAL Call No.
QL614 J68
Anaesthesia with MS-222 followed by intra-peritoneal (ip) injection (with
a 10% ethanol in corn oil carrier) of fathead minnow either as one or three
(weekly) treatments did not affect survival, behaviour or secondary sexual
characteristics of the fish. Fecundity of the fish, as indicated by fertility
and hatching success, was also unaffected. Gonadal condition (relative gonad
mass, histopathology) was not altered in either sex. Male and female plasma
sex steroids (beta -oestradiol, testosterone, 11-ketotestosterone) and male
vitellogenin concentrations were not significantly affected by the treatments.
Females subjected to either ip treatment regime had significantly higher plasma
vitellogenin concentrations than control females. However, based on previous
data, this difference did not appear to be treatment-related. Overall, exposure
of fathead minnows to chemicals via the ip route should not confound the interpretation
of toxicity tests with potential endocrine disrupting chemicals.
Descriptors: reproductive behavior, methodology, fish handling,
anaesthetics, survival, bait culture, behaviour, secondary sexual characters,
fecundity, fish physiology, animal behavior, sexual reproduction, toxicity,
Pimephales promelas, fathead minnow, fathead minnows
ASFA; Copyright © 2003, FAO
Kaiser H, Vine N (1998) The
effect of 2-phenoxyethanol and transport packing density on the post-transport
survival rate and metabolic activity in the goldfish, Carassius auratus.
Aquarium Sciences and Conservation. 2(1):1-7
ISSN: 1357-5325
To test the hypothesis that the anaesthetic 2-phenoxyethanol would reduce the
metabolic rate and allow for higher transport packing densities, goldfish (3.93
plus or minus 1.99 g) were transported for 48 h at 25, 50 and 75 fish per 500
ml combined with anaesthetic concentrations of 0, 0.25 and 0.35 ml/l. The
anaesthetic did not affect the survival rate or the oxygen and ammonia
concentrations. Thus, its use could not be recommended for the transport of
goldfish. It is suggested that optimum packing densities be based on a minimum
post-transport oxygen value of 4 mg/l for goldfish.
Descriptors: fish physiology, respiration, oxygen
consumption, dissolved oxygen, live
storage, transportation, ornamental fish, aquarium culture, freshwater fish,
anaesthetics, Carassius auratus
ASFA; Copyright © 2003, FAO
Kaminski R, Myszkowski L, Wolnicki
J (2001) Response to 2-phenoxyethanol in juvenile Vimba vimba (L.).
Archiwum Rybactwa Polskiego/Archives of Polish Fisheries.
NAL Call No. SH293 P7A73
In
Descriptors: alcohols, fry, juveniles, Vimba vimba,
anaesthesia, anaesthetics, phenols, freshwater fish, freshwater aquaculture
ASFA; Copyright © 2003, FAO
Kaneko K (1982) On the removal
of larger freshwater fishes. Biennial Report of the
Because of reconstruction of the tank where Arapaima and other freshwater
fishes had been kept, it was necessary to temporarily remove them to another
tank. Arapaima and Lepisosteus have the gas bladder well developed for aerial
respiration. Some anesthetic tests were examined with such physostomous fish as
Protopterus and Channa prior to the removal. In practical uses, 10% -
Quinaldine was added to the water in concentration of 100 ppm. The 2
anesthesized Osteoglossum was removed at first by handling with a blanket, and
the 3 species of catfish were succeeded in the same manner. The concentration
amounted to 150 ppm. The 3 Mylopharyngodon was removed. Arapaima and
Lepisosteus still kept their air-breathing, so 200 ppm-MS-222 and 20%-Fluothane
were sprayed at their gills as a supplementaly anesthetic treatment.
Descriptors: aquaria, fish handling, anesthesia,
freshwater fish
ASFA; Copyright © 2003, FAO
Kazun K, Siwicki AK (2001) Propiscin
-- a safe new anaesthetic for fish. Archiwum Rybactwa Polskiego/Archives
of Polish Fisheries. Olsztyn. 9(2):183-190
NAL Call No. SH293 P7A73
Anaesthetics are needed when handling fish, especially during tagging. However,
most anaesthetics applied at present have a strong toxic effect on fish. For
this reason it is only permissible to keep fish anaesthetized for a short time.
A new anaesthetic, Propiscin, which allows fish to be anaesthetized for up to
0.5 h, has been successfully tested in Poland. It contains a 0.2% stabilized
solution of etomidate and can be used as a bath. When administered correctly,
the required disappearance of sense perception and motor reflexes in the fish
can be obtained in about 2-4 min. The low toxicity of this pharmacological
confection has been proved according to a full set of clinical, toxicological,
hematological and biochemical criteria. Clinical tests have been conducted on
with many fish species, mainly salmonids.
Descriptors: anaesthetics, anaesthesia, freshwater aquaculture,
pharmacology, drugs, fish culture, fish handling, toxicology, Propiscin
ASFA; Copyright © 2003, FAO
Keene JL, Noakes DLG,
NAL Call No. SH1 F8
The anaesthetic effects of clove-oil-derived eugenol
were studied in juvenile rainbow trout, Oncorhynchus
mykiss (Walbaum). Acute lethality and the effects of multiple exposures to
eugenol were measured. The estimated 8-96 h LC sub(50)
for eugenol was found to be approximately 9 p.p.m. Times to induction and
recovery from anaesthesia were measured and compared with MS-222 under similar
conditions. Eugenol generally induced anaesthesia faster and at lower
concentrations than MS-222. The recovery times for fish exposed to eugenol were
six to 10 times longer than in those exposed to similar concentrations of
MS-222. Clove oil eugenol was determined to be an acceptable anaesthetic with
potential for use in aquaculture and aquatic research. Doses of 40-60 p.p.m. eugenol were found to induce rapid anaesthesia with a
relatively short time for recovery in juvenile trout.
Descriptors: fish culture, anaesthetics, lethal effects,
recovery, Oncorhynchus mykiss, eugenol, fish culture, anaethestics
ASFA; Copyright © 2003, FAO
Klaverkamp JF, Lockhart WL, Metner
WL, Grift N (1976) Effects of chronic DDT/DDE exposure on anesthetic induction
and recovery times in rainbow trout (Salmo gairdneri). Journal of
the Fisheries Research Board of Canada. 33(6):1331-1334
NAL Call No: 442.9 C16J
In rainbow trout (S. gairdneri) fed
pellets containing 4.55{mu}g/g of p, p'-DDT and 6.81{mu}g/g of p, p'-DDE every
other day. Anesthetic induction and recovery times of phenoxyethanol (PE) were
less than those in trout fed control pellets. No interactions were observed in
fish fed DDT/DDE and anesthetized with ethyl m-aminobenzoate methane sulphonate
(M.S. 222). Differences observed between fish fed DDT/DDE and anesthetized with
PE as compared to M.S. 222 could be due either to enhanced metabolism of PE or
to the fact that PE and M.S. 222 have different modes or sites of action.
Descriptors: insecticides, DDT, anaesthetics, Oncorhynchus
mykiss
ASFA; Copyright © 2003, FAO
Knoph MB (1995) Effects of
metomidate anaesthesia or transfer to pure sea water on plasma parameters in
ammonia-exposed Atlantic salmon (Salmo salar L.) in sea water. Fish
Physiology and Biochemistry. 14(2):103-109
ISSN: 0920-1742
Atlantic salmon (Salmo salar L)
postsmolts weighing 150 plus or minus 53 g were exposed to 14-15 mg/l TA-N
(total ammonia-N) in sea water in 1 m super(3) tanks for 24h. Blood samples
were then taken A) immediately after the fish were netted from the exposure
tanks and stunned by a blow to the head; B) 2-20 min after the fish were
transferred to 15 l of an anaesthetic solution of metomidate in “ammonia-free”
sea water; or C) 2-20 min after the fish were transferred to 15 l of
"ammonia-free" sea water. Plasma TA-N level was 18% lower in the
anaesthetised fish compared to in the fish sampled directly from the exposure
tanks (p less than or equal to 0.05), and accordingly 16% lower in the fish
transferred to pure sea water although this difference was not significant (p =
0.07). Plasma glucose level was higher in the fish transferred to pure sea
water than in the fish receiving the two other treatments (p less than or equal
to 0.05), but plasma urea, osmolality, Na+, Cl-, Ca++
or Mg++ levels did not vary significantly between the difference
treatments. Plasma TA-N level increased with time in the fish in the metomidate
solution (p less than or equal to 0.02).
Descriptors: fish physiology,
anesthesia, Salmo salar, blood,
ammonia, hematology, biological sampling, metomidate
ASFA; Copyright © 2003, FAO
Kohbara J, Nanba K, Murachi S
(1987) The heart rate of carp anesthetized with tetraethylene glycol dibutyl
ether. Bulletin of the Japanese Society of Scientific Fisheries. 53(4):681
NAL Call No. 414.9 J274
An anesthetic effect on a carp (Cyprinus
carpio) produced by tetraethylene glycol dibutyl ether was discovered. To
clarify the physiological condition of carp under the anesthetic effect
produced by this agent, the heart rate was measured during the anesthetic
period and compared with the anesthetic effect produced by MS-222. Both MS-222
and tetraethylene glycol dibutyl ether produced an increase in the heart rate
at first. The heart rate of the carp anesthetized with MS-222 maintained a high
beat rate during the experiment. That affected by tetraethylene glycol dibutyl
ether, however, fell off to below the heart rate of the fish in normal
condition. It is estimated that the anesthetic mechanism of this agent is very
different from that of MS-222.
Descriptors: heart, anesthesia, Cyprinus carpio,
heart rate, tetraethylene glycol dibutyl ether
ASFA; Copyright © 2003, FAO
Korstrom JS, Birtwell IK, Piercey
GE, Spohn S, Langton CM, Kruzynski GM (1996) Effect of hypoxia, fresh water,
anaesthesia and sampling technique on the hematocrit values of adult sockeye
salmon (Oncorhynchus nerka). Canadian technical report of fisheries
and aquatic sciences/Rapport technique canadien des sciences halieutiques
et aquatiques. Imprint varies. 34 pp
The hematocrit values of adult sockeye salmon, Oncorhynchus nerka, were determined after the fish were subjected
to sublethal hypoxia in salt water under simulated estuarine conditions, a
residual oxygen bioassay, transfer from sea water to fresh water, and a lethal
dose of the anaesthestic MS-222. Irrespective of treatment, hematocrit values
determined in sequentially-collected aliquots of blood decreased in relation to
the elapsed sampling time. A systematic and significant error occurred due to
the ordinal number of sampling the hematocrit capillary tubes a maximum of
three tubes of blood should be collected per fish for hematocrit determination.
Hematocrit values of fish anaesthetized with 200 mg/L MS-222 were not
significantly higher than those from control fish. Adult male and female
sockeye salmon had similar hematocrit values. Hematocrit values were
significantly increased after exposure to longer (48h) but not to shorter (6hr)
term hypoxia. Hematocrit values were significantly elevated after 8.5 weeks,
but not after 2.5 weeks residence in fresh water following transfer from sea
water. The highest hematocrit values were from fish exposed to the residual
oxygen bioassay which subjected the salmon to the greatest hypoxic stress. The
utility of hematocrit values in relation to environmental stressors is
discussed.
Descriptors: hypoxia, fresh water, anaesthesia, adults,
haemoglobins, blood cells, oxygenation, bioassays, fish physiology, Oncorhynchus nerka
ASFA; Copyright © 2003, FAO
Kreiberg H (1992) Metomidate sedation
minimizes handling stress in chinook salmon. Proceedings, 1992 Meeting
of the Aquaculture Association Of Canada, 1-3 June, 1992, University of British
Columbia, Vancouver, BC. Bulletin of the Aquaculture
Association of
NAL Call No. SH37.B8
Use of a recently introduced fish anaesthetic, metomidate, for controlling
physiological response to stress from handling procedures in chinook
salmon Oncorhynchus tshawytscha is described. Fish given a pre-handling
exposure to metomidate responded to a crowding and netting procedure with
relatively little elevation of plasma cortisol compared to other fish handled
without metomidate. Plasma cortisol in the metomidate-treated fish rose from resting
level of 28.4 plus or minus 1.8 ng/mL to 61.0 plus or
minus 7.5 ng/mL, compared to 204.9 plus or minus 16.2 ng/mL in untreated fish
(mean plus or minus 2SE).
Descriptors: fish culture, fish handling, biological
stress, therapy, drugs, Oncorhynchus tshawytscha, metomidate
ASFA; Copyright © 2003, FAO
Kreiberg H, Powell J (1991) Metomidate
sedation reduces handling stress in chinook salmon. World Aquaculture. 22(4):58-59 ISSN: 1041-5602
NAL Call No.
SH1.W62
Exposure to the recently introduced fish anaesthetic, metomidate, in the
fishes' home culture container prior to a handling stress resulted in
considerably reduced plasma corticosteroid response over untreated stressed
fish. A practical and inexpensive procedure in use since 1989 is described for
tanks, raceways and netpens. The unique sedative properties of metomidate are
considered to have broad potential benefit to posthandling health and survival
in sensitive fish such as chinook salmon. A requirement to handle live fish in
order to carry out procedures such as transportation, grading, or benign or
minor invasive sampling is recognized by many fish culturists as a potentially
stressful experience which may result in reduced vigor or mortality in the
affected stock. A number of researchers have described aspects of the
physiological consequences of handling procedures used with temperate and
coolwater fishes such as Pacific salmon species, various trout, striped bass
and red drum. Essentially, the fright-flight response is triggered, involving
adrenaline and subsequent impacts on the fish's ability to maintain its blood
constituents, osmoregulation and immune system preparedness. Most reports have
singled out the netting and capture phase of various procedures as the major
contributor to overall stress associated with a particular procedure. Reports
of the suitability of a recently introduced fish anaesthetic, metomidate, for
minimizing the stress response to handling in striped bass and Atlantic cod
suggested applications in the culture of Pacific salmon, particularly chinook
salmon. Over a number of practical trials with different sizes of chinook and
coho salmon, we developed a standard procedure for light sedation of fish
before a handling disturbance. This report provides a summary and evaluation of
our pre-handling technique. (DBO)
Descriptors: marine aquaculture, aquaculture techniques,
fish physiology, Oncorhynchus tshawytscha
ASFA; Copyright © 2003, FAO
Kumlu M, Yanar M (1999) Effects
of the anesthetic quinaldine sulphate and muscle relaxant diazepam on sea bream
juveniles (Sparus aurata). Israeli Journal of
Aquaculture/Bamidgeh. 51(4):143-147
NAL Call
No. SH117 I75B36
In this investigation, the effects of an anesthetic, quinaldine sulphate (QS), and
a muscle relaxant, diazepam (D), on sea bream juveniles (Sparus aurata) were studied. The application of diazepam
significantly increased the safety level of the anesthetic QS for the sea bream
juveniles. The fish entered light anesthesia at 5 ppm QS + 1 ppm D, as compared
to 10 ppm QS. Similarly, the deep anesthesia level was reached at only 7.5 ppm
QS + 1 ppm D as compared to 15 ppm QS. The use of QS alone at high
concentrations (15-20 ppm) resulted in mortality of 30% to 100%. No mortality
occurred in the fish treated with QS plus D at all anesthesia levels. Depending
on the anesthetic concentrations used, the time to loss of balance and the
recovery time were 0-2 min and 2-6 min, respectively. Administration of
diazepam with a lower concentration of QS significantly enhanced the
anesthesia, eliminated the undesirable effects of QS and reduced the excitement
and hyperactivity of the fish in the confined space, without leading to
mortality. Suitable light and deep sedation stages of anesthesia for transportation
and handling of sea bream juveniles (6-7 g) were obtained with dosages of 5 ppm
QS + 1 ppm D and 7.5 ppm QS + 1 ppm D, respectively.
Descriptors: anaesthetics, fish culture, aquaculture
techniques, fish larvae, survival, diazepam, anesthetics, quinaldine sulfate, Sparus aurata, quinaldine sulphate,
diazepam, gilthead seabream
ASFA; Copyright © 2003, FAO
Ladu BMB, Ross LG (1997) The effect
of methods of immobilization on the haematology and tissue chemistry of rainbow
trout Oncorhynchus mykiss Walbaum. Journal of Aquatic Sciences.
12:31-41
The influence of methods of immobilization on the haematology and tissue
chemistry of rainbow trout Oncorhynchus
mykiss was investigated. Both chemical and electroanaesthesia had similar
effects on haematology and tissue chemistry of the fish. Generally, however,
haemoconcentration was minimized by electroanaesthesia and it is recommended as
the preferred method of sampling because it is cheap, safe and less stressful.
The demands on respiration, nutrient metabolism, ionoregulation and
osmoregulatory activities were variously effected by each stressor.
Descriptors: haematology, fish physiology, tissues,
histochemistry, anaesthesia, biological stress, Oncorhynchus mykiss
ASFA; Copyright © 2003, FAO
Laidley CW, Leatherland JF (1988) Cohort
sampling, anaesthesia and stocking-density effects on plasma cortisol, thyroid
hormone, metabolite and ion levels in rainbow trout, Salmo gairdneri
NAL Call No. QL614 J68
The effect of serial removal of fish from aquaria, anaesthesia and stocking
density on plasma cortisol, thyroid hormone, metabolite and ion levels was
examined in rainbow trout, Salmo gairdneri, to determine the consequences of
normal handling and maintenance procedures on the activity of the
pituitary-adrenal and pituitary-thyroid axes in the species.
Descriptors: Salmo gairdneri, Oncorhynchus mykiss,
anaesthesia, fish handling, biological stress, fish physiology, stocking
density
ASFA; Copyright © 2003, FAO
Laitinen M, Nieminen M, Pasanen P,
Hietanen E (1981) Tricaine (MS-222) induced modification on the metabolism
of foreign compounds in the liver and duodenal mucosa of the splake (Salvelinus
fontinalis X Salvelinus namaycush). Acta Pharmacologica et
Toxicoogica. 49(2):92-97
The splake, a popular game fish, is a crossbreed which must be reared in
nurseries. The fish are marked under anaesthesia for later study. We anlayzed
the effect of a common anaesthetic, tricaine (MS-222), on the metabolism of
foreign compounds in the liver and duodenum of the splake. In the liver and to
some extent in the duodenum, aryl hydrocarbon hydroxylase and epoxide hydrase
activities were reduced during treatments. The ethoxycoumarin O-deethylase activities
were not affected in either the liver or duodenum. Tricaine significantly
decreased the hepatic UDP-glucuronosyltransferase activity. The decrease was
greater when the aglycone used was p-nitrophenol than with methylumbelliferone.
A similar effect was also found after trypsin treatment of the microsomes. No
significant decrease in the UDP-glucuronosyltransferase activity was detected
in the duodenal mucosa. This was the case when both p-nitrophenol and
methylumbelliferone were used as aglycones.
Descriptors: anaesthetics, enzymatic activity, liver,
intestines, Salvelinus fontinalis, Salvelinus namaycush, MS-222
ASFA; Copyright © 2003, FAO
Lambooij E, Vis JW, van de
Kloosterboer RJ, Pieterse C (2002) Welfare
aspects of live chilling and freezing of farmed eel (Anguilla anguilla):
neurological and behavioural assessment.
Aquaculture. 210 (1/4):159-169
NAL Call No. SH1 A6
The overall objective of the study was to evaluate a
slaughter method of eels, which consisted of chilling until their body temperature
was <5oC for stunning, and subsequently placing them in cold brine at -18oC
for 15 min for killing. Three distinct experiments and a control were
performed. First, 19 eels with an average live weight of 758 " 44 g were restrained and equipped with EEG, ECG
electrodes and a temperature sensor inside the body. Then, they were placed in
the ice water. Indices for the induction of unconsciousness and insensibility
were the appearance of theta and delta waves and no response on pain stimuli,
which disappeared at a body temperature of 8.0 " 2.1oC after 12 " 5 min in 15 eels. The responses to pain stimuli did
not disappear in three eels. Within a confidence level of 95%, the percentage
of eels that was not effectively stunned during the procedure in ice water of
<5 oC was at least 5%. The heart rate decreased from 24 " 10 beats/min (n=14) to 7 " 4 (n=11) and became irregular during cooling down.
When placed in the brine water of -18oC, the EEG showed rapid and
extreme depolarization of the membranes, which started after 27 "17 seconds (n=18). The ECG showed fluttering of the
heart in all eels. None of the eels recovered after this procedure. For 10 eels
with an average live weight of 128 " 27 g, it was observed that the body temperature
decreased from 17.1 " 0.6 to 4.0 " 0.5oC in the ice water. After 15 min in
the brine water of -16.1 " 2.2oC, the body temperature decreased to
-3.1 " 2.3oC. Finally, three groups of 7 eels and 8 single eels
were placed in ice water of -0.0 " 0.1oC. The observation of unrestrained
eels revealed four phases. Animals were (1) swimming around in the water, (2)
attempting to escape from the ice water, (3) pressing their nose to the wall or
corner while showing clonic muscle cramps, and finally (4) breathing only,
while all other muscle activity was totally suppressed. Afterwards, they were
transferred to cold brine at -18oC, and none of the eels recovered.
The eight control eels, which were transferred to water at 18oC swam
around, except for one that was lying in an S-shape position at the bottom.
After 570 and 605 seconds, two eels tried to escape from the box. The obtained
results showed that the eels, which were transferred from water at 18oC
to ice water, might be stressed, a specific behaviour and an irregular heart
rate were observed. From an animal welfare point of view, it is therefore not
recommended to stun eels by live chilling. Moreover, at least 5% of the eels
will not be stunned at a body temperature of <5oC. Placing eels
in brine water of -18oC is an effective method to kill the eels
before slaughter. However, it cannot be recommended to place conscious eels in
cold brine water, because it takes more than 27 seconds before unconsciousness
may be induced.
Descriptors: animal behaviour, animal welfare, body temperature,
chilling, freezing, heart rate, neurology, pain, slaughter, stunning, eels,
Anguilla, Anguillidae, Anguilliformes, Osteichthyes, fishes, diadromous fishes,
aquatic animals, aquatic organisms
Copyright © 2003, CAB International.
Le Bras YM (1982) Effects of
anaesthesia and surgery on levels of adrenaline and noradrenaline in blood
plasma of the eel (
NAL Call No. QP1 C6
The effects of surgery and anaesthesia on adrenaline
and noradrenaline plasma levels were investigated in the eel (
Descriptors: anaesthesia, biological stress, blood,
ASFA; Copyright © 2003, FAO
Lewis DH, Tarpley RJ, Marks JE,
Sis RF (1985) Drug induced structural changes in olfactory organ of channel
catfish Ictalurus punctatus, Rafinesque. Journal of Fish Biology.
26(3):355-358
NAL Call No. QL614 J68
The fish anesthetic tricaine methanosulfate destroyed the cilia on olfactory
sensory epithelia of channel catfish (I.
punctatus) when fish were exposed to tranquilizing doses of the drug. Cilia
on the nonsensory epithelium appeared to be unaffected by multiple exposures of
the drug. Sensory cilia regenerated within 28 days after exposure.
Descriptors: anesthetics, histopathology, olfactory
organs, Ictalurus punctatus
ASFA; Copyright © 2003, FAO
Limsuwan C, Grizzle JM, Plumb JA
(1983) Etomidate as an anesthetic for fish: Its toxicity and efficacy. Transactions
of the American Fisheries Society. 112(4):544-550
NAL Call No. 414.9 AM3
Etomidate was tested as an anesthetic on channel catfish Ictalurus punctatus,
golden shiners Notemigonus crysoleucas, and bluegills Lepomis macrochirus. The
24-hour median lethal concentration (LC50) at various temperatures was lowest
for bluegills (0.61-0.68 mg/liter) and highest for golden shiners (1.87-2.73
mg/liter). Generally, etomidate was more toxic, and induction of anesthesia and
recovery from anesthesia were slower, at 17oC than at 22 oC
and 27 oC. Both channel catfish and golden shiners were anesthetized
within 15 minutes by 3 mg/liter, and all of the fish survived at this
concentration for 30 minutes. concentrations of 0.2 and 0.4 mg/liter sedated
channel catfish, and 0.4 and 0.6 mg/liter sedated golden shiners. The safety
index LC50/EC50 (median effective concentration) for anesthesia of channel
catfish and golden shiners at 22oC " 1oC decreased from 6.2 to 5.0 and 7.6 to
4.0, respectively, as exposure length increased from 10 to 80 minutes.
Descriptors: anesthetics, toxicity, Ictalurus
punctatus, Notemigonus crysoleucas, Lepomis macrochirus,
utilization, evaluation, etomidate
ASFA; Copyright © 2003, FAO
Limsuwan C, Limsuwan T, Grizzle
JM, Plumb JA (1983) Stress response and blood characteristics of channel
catfish (Ictalurus punctatus) after anesthesia with etomidate. Canadian
Journal of Fisheries and Aquatic Sciences. 40(12):2105-2112
NAL Call No: 442.9 C16J
Continuous anesthesia of channel catfish (Ictalurus
punctatus) with 0.6 mg/L etomidate for 96 h caused a small but statistically
significant decrease in plasma protein concentration at all sampling periods.
Anesthetized fish were not stressed by the periodic sampling. Fish anesthetized
with 3 mg/L etomidate and then confined in a net for 10 min had reduced plasma
cortisol response and no significant plasma glucose increase compared with
unanesthetized controls. Anesthesia did not prevent hyperchloremia that
developed 3 h after the 10-min confinement. No histological changes were found
in fish anesthetized with etomidate. Anesthesia with etomidate before netting
could be useful when handling fish because of the reduced stress response.
Descriptors: anaesthesia,
fish culture, haematology, histology, biological stress, Ictalurus punctatus
ASFA; Copyright © 2003, FAO
MacAvoy SE, Zaepfel RC. ( 1997) Effects of tricaine
methanesulfonate (MS-222) on hematocrit: First field measurements on blacknose
dace. Transactions of the American Fisheries Society. 126(3):500-503
NAL Call No. 414.9 AM3
Tricaine methanesulfonate (MS-222) is an anesthetic commonly used to reduce
fish stress during transport or sampling. The exposure of blacknose dace
Rhinichthys atratulus to MS-222 at concentrations of 300 mg/L or 500 mg/L for 9
min or less did not raise hematocrit levels above those of controls. Hematocrit
levels tended to be somewhat lower in experimental groups than in controls;
however the only significant difference (P = 0.044) among treatments occurred
between the control fish (35% " 2.3; N = 6) and fish exposed to 500 mg/L for 3 min
(30% "1.8; N = 7). This difference suggests that initial exposure to MS-222
may cause stress or, less likely, that the anesthetic has some inherent
hemodilution effect. Workers who monitor environmental acidification may be
concerned with the hematocrit of the acid-sensitive blacknose dace because
hematocrit increases during acid stress. The use of MS-222 to ease hematocrit
sampling should not elevate measurements.
Descriptors: anaesthetics, blood, biological stress,
therapy, acidification, pollution indicators, anesthetics, hematocrit, stress,
USA, Virginia, Blue Ridge, Paine Run, tricaine methanesulfonate, Rhinichthys
atratulus, USA, Virginia, Blue Ridge Mts., Paine Run, therapy, Blacknose
dace
ASFA; Copyright © 2003, FAO
MacKinlay DD, Johnson MVD, Celli
DC (1994) Evaluation of stress of carbon dioxide anaesthesia. High
Performance Fish: Proceedings of an International Fish Physiology Symposium
at the University of British Columbia in Vancouver, Canada, July 16-21 1994.
Fish Physiology Association, Vancouver, BC (Canada). pp. 421-424
NAL Call No.
QL639.1 I58 1994
The Salmonid Enhancement Program (SEP) has applied coded-wire tags to 7-12
million fish per year since the early 1980's, an operation that requires
short-term (5-10 min) anaesthesia. Tricaine methane sulphonate (TMS) and
2-phenoxyethanol (TPE) were the chemicals most often used in the early years of
the SEP. Several concerns, including the safety of hatchery workers using TPE,
warnings about releasing fish into seawater soon after exposure to TMS and
increasing restrictions on the availability of drugs for routine procedures has
led to a switch to carbon dioxide gas as the preferred anaesthetic at SEP
hatcheries. One of the marked differences between anaesthesia with carbon
dioxide and other anaesthetics is that fish exhibit an extreme hyperactive
response when first immersed in water containing a high concentration of carbon
dioxide. This led to concerns that the fish were extraordinarily stressed by
carbon dioxide anaesthesia, which led to this series of experiments to
determine if the carbon dioxide caused a greater degree of measurable
physiological stress than the other anaesthetics. Cortisol was chosen as the
index of stress, in accordance with common practice (Donaldson, 1981).
Descriptors: biological stress, tagging, anaesthesia,
carbon dioxide, fish physiology, fish handling, Salmonidae, fish culture
ASFA; Copyright © 2003, FAO
Malmstroem T, Salte R, Gjoeen HM,
Linseth A (1993) A practical evaluation of metomidate and MS-222 as
anaesthetics for Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture.
113 (4):331-338
NAL Call No. SH1A6
Metomidate and MS-222 were tested as anaesthetics for
Atlantic halibut (Hippoglossus
hippoglossus L.) at temperatures of 9.5 "0.3°C and 10.5 " 0.3°C. An effective concentration of the drug was
defined as one giving a sure state of anaesthesia for 75% of the fish after an
exposure time of less than 5 min. The lowest effective concentration of
metomidate was 10 mg/l; with MS-222, concentrations of 250 mg/l were required.
Doses should further be kept below 60 mg metomidate/l or 480 mg MS-222/l. There
was interaction between anaesthetic and dose for both drugs with respect to the
required exposure time, which focuses on the necessity of testing several doses
when a new anaesthetic is to be used on a given species. In is concluded that
metomidate gives a broader safe anaesthetic range with a lower effective dose
than MS-222.
Descriptors: fish culture, aquaculture techniques,
pharmacology, anaesthetics, comparative studies, Hippoglossus hippoglossus,
metomidate, MS-222
ASFA; Copyright © 2003, FAO
Marx H, Brunner B, Weinzierl W, Hoffmann
R, Stolle A (1996) Comparative investigations on different methods for
stunning fish with special regard to meat quality parameters. Proceedings of the Conference of IIR Commission C2,
Taking into account aspects of meat quality and animal welfare, three
methods for stunning fish were compared: manually (blow on the head, stab in
the neck) with electricity and using CO2. The following results were
obtained for eel (n = 72), carp (n = 120) and trout (n = 54). From the view of
animal welfare, the effects on the fish were judged. Excitation and mucus
secretion, as weIl as the period of lime until the
fish were in anaesthesia were recorded. With manual and electrical stunning,
aIl fish were anaesthetized almost immediately, while using CO2, it
takes 3.2 min (trout), 9.2 min (carp) and 109.7 min (eel), on average. After
slaughter, after three and eight days of storing the fish on ice, the meat
quality parameters, pH value, water holding capacity and rigor mortis were
measured. CO2 stunning showed the lowest pH-values and water holding
capacities; also, rigor mortis in carp and eel advanced most. Testing of raw
and prepared fish was performed by a sensoric team. In many cases, fish
anaesthetized manually were ranked better than the
other groups. The findings indicate that CO2 was not appropriate for
stunning carp and eel. Electrical stunning, with some improvements, could meet
the requirements of meat quality and animal welfare.
Descriptors: processing fishery
products, anaesthesia, slaughter, quality, Oncorhynchus mykiss, Cyprinus carpio,
ASFA; Copyright © 2003, FAO
Massee KC, Rust MB, Hardy RW,
Stickney RR (1995) The effectiveness of tricaine, quinaldine sulfate and
metomidate as anesthetics for larval fish. Aquaculture.
134(3-4):351-359
NAL Call No. SH1A6
Tricaine, quinaldine sulfate, and metomidate were
compared as anesthetics for larvae of two species of fish, red drum (Sciaenops ocellatus) and goldfish (Carassius auratus). Larvae were exposed
to various concentrations of each anesthetic and the percentages of fish
reaching stage 4 of anesthesia, post-exposure recovery, and survival were
recorded. Effective concentrations were defined as those which induced stage 4
of anesthesia within 3 min after exposure with a recovery time of 10 min or
less. Post-exposure survival of 100% was an additional criteria used to define
effective anesthetic concentrations. The lowest effective concentration of
tricaine for red drum was 55 mg/l (26°C), while 50 mg/l (24°C) was the lowest
effective concentration for goldfish. The lowest effective concentration of
quinaldine sulfate for red drum was 35 mg/l (26°C), while 60 mg/l (24°C) was
the lowest effective concentration for goldfish. Metomidate was found to be an
ineffective anesthetic for both red drum and goldfish larvae based upon
survival and recovery times. Mortality occurred in red drum larvae at all
tested concentrations of metomidate. Larvae of both species that survived
anesthesia with metomidate had longer induction and recovery times compared to
larvae exposed to tricaine and quinaldine sulfate.
Descriptors: fish larvae, anesthetics, survival, Sciaenops
ocellatus, Carassius auratus, tricaine, quinaldine sulfate, metomidate
ASFA; Copyright © 2003, FAO
Matthews GM, Paasch NN, Achord S,
McIntyre KW, Harmon JR (1997) A technique to minimize the adverse effects
associated with handling and marking salmonid smolts. Progressive Fish
Culturist. 59(4):307-309
NAL Call No. 157.5 P94
A system that allows anesthetization of juvenile salmonids before netting
during a handling and marking operation is described. Our purpose for designing
the system was to reduce or minimize any debilitating effects associated with
these activities. When compared with smolts handled and marked in the
traditional manner, use of the technique resulted in a significant reduction in
the mortality of naturally migrating smolts of spring-summer chinook salmon
Oncorhynchus tshawytscha during a posthandling and marking seawater challenge
performance test. The treatment resulted in lower, but not significantly lower,
plasma cortisol levels; however, sample sizes may have been too small for
statistical verification. With a little ingenuity, the technique should be adaptable
to most smolt handling or marking operations.
Descriptors: smolts, aquaculture techniques, marking,
anaesthesia, fish culture, fish handling, biological stress, Oncorhynchus
tshawytscha
ASFA; Copyright © 2003, FAO
Mazur CF, Boreham A, McLean W, Iwama
GK (1991) Rectified wide band white noise as an electroanaesthesia waveform
for use with rainbow trout (Oncorhynchus mykiss). ICES Council
Meeting Papers, ICES, Copenhagen (Denmark). 28 pp.
Rectified and pulsed wide band white noise shows promise as an effective
waveform for use in salmonid electroanaesthesia. The use of electroshocking for
the capture of fish has often resulted in muscular tetany and vertebral
displacement leading to paralysis and death. Wide band white noise used in
mammalian electroanaesthesia has been shown to reduce muscular tetany and was
tested here for use with salmonids. Trials on rainbow trout (Oncorhynchus mykiss) and coho salmon (O. kisutch) revealed that pulsed
rectified wide band white noise (5-30 KHz), produced by a noise generator and a
wide band amplifier, induced anaesthesia and reduced, but did not eliminate,
damaging tetanic contractions.
Descriptors: anaesthesia, electrophysiology, Oncorhynchus
mykiss, Oncorhynchus kisutch, INE, Canada, British Columbia
ASFA; Copyright © 2003, FAO
Mazur CF, Yesaki TY, Iwama GK (1991)
Improvements on the use of two alternatives to chemical anaesthesia for
fish: Electroanaesthesia and CO2 anaesthesia. Ices Council
Meeting Papers, Ices, Copenhagen (Denmark). 1 p.
There is an increasing need for the use of non-chemical anaesthesia for
fish cultured for food as well as for wild fishery enhancement. This need
arises from the potential impacts of chemicals on the environment, as well as
the requirement to reduce chemical residues in food fish. This paper describes
work that we have conducted in the refinement of the use of two alternatives to
chemical anaesthetics. While both electroanaesthesia and CO2
anaesthesia have been used for fishery and aquaculture purposes, both have
drawbacks in their use in fish. Severe muscle tetany can result from
electroanaesthesia, often resulting in spinal dislocation and muscle
hemorrhaging. CO2 anaesthesia causes irritation and a struggle
response in exposed animals.
Descriptors: anaesthetics, bioaccumulation, tissues,
environmental impact, aquaculture effluents, Oncorhynchus
ASFA; Copyright © 2003, FAO
McNeil FI, Crossman EJ (1979) Fin
clips in the evaluation of stocking programs for muskellunge, Esox masquinongy. Transactions of
the American Fisheries Society. 108(4):335-343
NAL Call No. 414.9 AM3
During laboratory and field experiments in Ontario,
with hatchery muskellunge 90-235 mm in total length, total removal of a fin did
not add to the immediate mortality caused by seining the fish from ponds. The
use of an anesthetic during surgery (MS-222) did not affect subsequent survival
of marked, stocked fish. Removal of any single paired fin was equally
detrimental to short-term (3 months) survival. In contrast, over long periods
(10 months) the loss of a pectoral fin was more detrimental than loss of a
pelvic fin. Removal of both fins of a pair may cause higher mortality than the
removal of one fin. Neither the fin removed nor the anesthetic significantly
affected short-term or long-term growth. Within 1 year of marking regeneration
of amputated fins was such that recognition of marked fish was difficult and
the degree of difficulty increased with time. Estimates based on marked
2-year-old or older individuals could rsult in substantial underestimates of
survival.
Descriptors: stocking (organisms), tagging mortality, Esox
masquinongy, tagging, mortality causes, anaesthetics, survival, Esocidae,
Pisces
ASFA; Copyright © 2003, FAO
McVicar AJ, Rankin JC (1983) Renal
function in unanaesthetized river lampreys (Lampetra fluviatilis L.):
Effects of anaesthesia, temperature and environmental salinity. Journal
of Experimental Biology. 105:351-362
NAL Call No.
442.8 B77
Improved estimates of urine flow rates of lampreys in various salinites were
obtained by the collection of urine for periods of up to 48 h from
minimally-stressed, unanaesthetized fish, following catheterization of the
urinogenital papilla. Urine flow rate in unanaesthetized fish was extremely
sensitive to rapid (6°C/hour) changes in temperature and Q10
(6-16°C) was approximately 5. Urine flow rate decreased rapidly as the osmotic
difference between the body fluids and environment approached zero, and the
rate of flow in 30% seawater lampreys was only 7 multiplied by 6% that of
freshwater fish. Present data compare favourably with data obtained previously
from anaesthetized animals, indicating that renal function in lampreys is not
significantly impaired by light MS222 anaesthesia.
Descriptors: urine, excretion, temperature effects,
salinity effects, anesthetics, Lampetra fluviatilis
ASFA; Copyright © 2003, FAO
Mgbenka BO, Ejiofor EN (1998) Effects
of Extracts of Dried Leaves of Erythrophleum suaveolens as Anesthetics on
Clariid Catfish. Journal of Applied Aquaculture. 8(4):73-80
NAL Call No. SH135 J69
The effects of crude extract, pure extract, aqueous fraction of pure extract
and lipid fraction of pure extract of air-dried leaves of Erythrophleum suaveolens as anesthetic on African sharptooth, Clarias gariepinus, and the African
vundu catfish, Heterobranchus longifilis,
fingerlings were studied. They were exposed to various doses of the extracts in
tanks. The time for each fish to reach anesthesia were recorded. The two
clariids were anesthetized in up to 3.5 g/L crude extract and recovered in the
fresh water. Soaking the leaves for 24 hours or 48 hours produced no
significant difference (P > 0.05) in the time to reach anesthesia for the
African vundu catfish. These fingerlings reached anesthesia in significantly
shorter time (P < 0.05) (24.5 minutes at 2.4 g/L concentration) in the pure
unseparated extract than in the crude extract (70.5 minutes at 2.4 g/L
concentration). All fingerlings exposed to 4 g/L extracts did not recover.
Those exposed to less than 3.5 g/L of plant material were anesthetized and
recovered only to die later within 24 hours. The time to reach anesthesia
decreased with an increase in concentration of the plant extract. Of the two
fractions, only the lipid fraction had anesthetizing effect on fish. It,
however, took longer to produce the effect than the unseparated pure extract.
The aqueous fraction of the pure extract and the control produced no observable
anesthetic effects on the fish within 180 minutes. This suggests that the
anesthetizing active ingredient resided in the lipid fraction but some factor
in the aqueous layer was necessary to quicken its action. Similar results were
got with the sharptooth catfish. Since the fingerlings died after recovering
from anesthesia it was concluded that the safety margin of E. suaveolens for fingerlings was very narrow at the concentrations
used. It is, therefore, not recommended for use on the fingerlings of the
clariid catfishes.
Descriptors: fish culture, anaesthetics, fish handling,
biological stress, Erythrophleum suaveolens, Clarias gariepinus,
Heterobranchus longifilis
ASFA; Copyright © 2003, FAO
Milton P, Dixon R.N (1980) Further
studies of the effects of the anaesthetic quinaldine on the physiology of the
intertidal teleost Blennius pholis.
Journal of the Marine Biological Association. UK. 60(4):1043-1051
NAL Call No.
442.9 M331
Marked reductions in oxygen consumption were noted in high concentration of
quinaldine (10 and 20 ppm), and both entry into anaesthesia and recovery from
it were rapid. A period of enhanced oxygen consumption followed anaesthesia,
except in the lowest concentration of quinaldine (1 ppm). Experiments conducted
over a 4 h period with three different salinities (100, 30 and 10% sea water)
indicated that, under the influence of 10 and 20 ppm quinaldine solutions, the
fish more resembled an osmoconformer than an osmoregulator. During anaesthesia,
water was lost osmotically in 100% sea water, and gained in the more dilute
salinities, although it was possible that some osmotic regulation continued.
Physiological measurements indicated that quinaldine is suitable for the
capture and marking of fish; for surgical procedures it should be mixed with
another anaesthetic, for example MS-222, due to the retention of a response to
vibratory stimuli.
Descriptors: anaesthetics, oxygen consumption, Blennius
pholis, acclimatization, intertidal environment, Blenniidae, Pisces
ASFA; Copyright © 2003, FAO
Mishra BK, Kumar D, Mishra R
(1983) Observations on the use of carbonic acid anaesthesia in fish fry
transport. Aquaculture. 32(3-4):405-408
NAL Call No. SH1A6
Observations are reported on the use of carbonic acid
anaesthesia in fish fry transport. The results indicate that live fry of Labeo rohita (Ham.) could be kept safely
(with 95% survival) in the transport medium under a dose of 500 ppm of carbonic
acid for as long as 215 h. Controls survived for only < 106 h.
Descriptors: stocking (organisms), fish larvae,
anaesthetics, carbonic acid, survival, Labeo rohita
ASFA; Copyright © 2003, FAO
Morales AE, Garcia-Rejon L, Higuera
Mde la, Billard R, Pauw Ndec (1989) Use of anaesthesia in situ for handling
stress suppression in rainbow trout. Aquaculture Europe '89. Short
Communications and Abstracts of Review Papers, Films/Slideshows and Poster
Papers, Presented at the International Aquaculture Conference held in Bordeaux,
France, 2-4 October, 1989, 1989, Special Publication, European Aquaculture
Society. 10:173-174
NAL Call No. SH138.S64
The findings are presented of experiments conducted to determine the effects of
in situ anaesthetization of cultured rainbow trout (Oncorhynchus mykiss) on suppressing stress caused by routine
laboratory handling of the fish. It is suggested that anaesthetics may be used
to avoid stress responses under laboratory conditions, but not in fish farms.
Descriptors: laboratory culture, fish handling, biological
stress, anaesthesia, Oncorhynchus mykiss, fish culture
ASFA; Copyright © 2003, FAO
NAL Call No. QL614 J68
The efficacy of quinaldine, benzocaine, MS-222,
2-phenoxyethanol and clove oil was compared for anaesthetizing settlement stage
Pomacentrus amboinensis, a frequently studied coral reef fish. Induction to
anaesthesia, behaviour during anaesthesia, recovery times and survival rates of
fish treated with the different chemicals were compared. Clove oil was only marginally
less effective than quinaldine and was more effective than other chemicals
tested, except at high concentrations. In addition, fish exposed to clove oil
exhibited a much calmer induction to anaesthesia than fish exposed to
quinaldine. Therefore, clove oil may be an effective alternative to quinaldine
as a fish anaesthetic. Recovery time after anaesthesia with clove oil was two
to three times longer than recovery from other chemicals, a desirable
characteristic for use in field studies. Survival rates were excellent for all
chemicals.
Descriptors: anaesthesia, marine fish, coral reefs,
survival, developmental stages, anesthetics, Pomacentrus amboinensis,
clove oil, quinaldine, Pallid damselfish
ASFA; Copyright © 2003, FAO
Muzinic R (1970) On the use of
anaesthetics in the transportation of sardines. Studies
and Reviews of the General Fisheries Council of the
The rate of mortality of non-selected sardines,
exposed to 1:150,000 conc of tricaine methane sulfonate in open-system experiments
increased rapidly and after 2 hr, by far exceeded 50 per cent. The mortality
rate of the fish slowed down considerably when the sardines were transferred to
a fresh anaesthetic solution at 30 min intervals using a conc of 1:150,000
tricaine methane sulfonate or compressed air when the temps were 20.8°C and
21.7°C; transferring the sardines had the same effect on their mortality using
chloral hydrate at both 1:1,000 and 1:3,000 concs and the effect was even more
notable at the latter concs. Similar procedure may be applied in transporting
sardines, especially from distant localities for tagging and other experimental
work. It is possible that some changes in the composition of the anaesthetic
solution during the initial phase of transportation may be useful. With changes
in the anaesthetic solution being made at 30-min intervals, lower concs of
chloral hydrate were more advantageous. In standard anaesthesia experiments
however, this was not so. In standard anaesthesia experiments with chloral hydrate,
a rapid increase in mortality occurred at a decline of the O2 conc
to a point below 2 cc/1. In open system standard
anaesthesia experiments using 1:150,000 tricaine methane sulfonate conc, the
last sardine died at a temp ranging from 20.3°C to 22.8°C and at a mean final O2
value of 0.56 + or - 0.46 cc/1. Chloral hydrate at 1:3,000 and 1:5,000 concs
(and perhaps even lower ones) may replace tricaine methane sulfonate in
transporting the sardines. The delicate state of the fish was shown by a marked
variability of the mortality course within all the series of anaesthesia
experiments and by a rather high mean final oxygen value and its great
variation.
ASFA; Copyright © 2003, FAO
Descriptors: anesthetic, anesthesia, tranport, sardines,
chloral hydrate
Nilsen H, Lillehaug A, Taksdal T,
Nordmo R (1992) Toxicity of intraperitoneally injected formalin in Atlantic
salmon, Salmo salar L. Journal of Fish Diseases. 15(4):323-329
NAL Call No. SH171 A1J68
Atlantic salmon, Salmo salar L., parr
were injected intraperitoneally with different quantities of formalin (37%
formaldehyde) following anaesthetization with either benzocaine
(ethyl-p-aminobenzoate) or chlorbutol (1,1,1,-trichloro-2-methyl-2-propanol).
The LD50 for injected formaldehyde was found to be approximately 50
mg/kg body weight. The type and concentration of the anaesthetic used did not
influence mortality rates. Formalin is commonly used to inactivate
microorganisms in vaccines. Doses of formalin which were shown to be toxic in
this study are close to those which may be injected into fish as a component of
various vaccines against bacterial fish diseases. Hence, the formalin content
in fish vaccines may well cause mortality under certain conditions.
Descriptors: fish diseases, bacterial diseases, disease
control, vaccination, fish culture, anaesthesia, Salmo salar, Atlantic
salmon
ASFA; Copyright © 2003, FAO
Obradovic J (1986) Effects of
anaesthetics (halothane and MS-222) on crayfish, Astacus astacus. Aquaculture
52(3):213-217
NAL Call No. SH1A6
The effects of an anaesthetic dispersive in air and
usually applied in mammals (halothane, Hoechst) and of an anaesthetic soluble
in water and applied in fish (MS-222, Sandoz) were investigated in experiments
with crayfish, A. astacus (L.).
Halothane was used at concentrations of 0.01, 0.06, 0.12, 0.5 and 1.0 vol. %,
while MS-222 was applied in two concentrations, dissolved in the ratios of
1:1000 and 1:10,000. Halothane was most effective at 0.5 vol. %. The
concentrations of MS-222 which were applied in the authors experiments had
almost no effect on the crayfish.
Descriptors: crayfish culture, aquaculture techniques,
anaesthetics, Astacus astacus, Halothane, MS-222
ASFA; Copyright © 2003, FAO
Oikawa S, Takeda T, Itazawa Y
(1994) Scale effects of MS-222 on a marine teleost, porgy Pagrus major.
Aquaculture 121(4):369-379
NAL Call No. SH1A6
The relationship between the effective concentration CE
of the anaesthetic MS-222 and body mass was examined at 20°C in porgy ranging in
size from 0.00022 g (just after hatching) to 320 g (595 days old). CE
was defined as the concentration which required 3-5 min to induce deep
anaesthesia in the fish. Values of CE (ppm) increased monophasically
with increasing body mass (W in g), following the equation CE = 79W
super(0.0549) (N = 30, r = 0.936 between log CE and log W). The
recovery ratio (R sub(R) in %), i.e., the number of fish as a percentage which
recovered after being placed back in ordinary seawater, varied depending on the
developmental stage. R sub(R) was lowest in fish of around 0.01 g (30 days
old), corresponding to the transitional stage between the post-larval and
juvenile stage, whereas it was highest (nearly 100%) in the pro-larval stage
(about 0.00023 g in size and 0-6 days of age).
Descriptors: anesthetics, body size, recovery, fish
culture, Pagrus major, anaesthesia, MS-222
ASFA; Copyright © 2003, FAO
Olsen YA, Einarsdottir IE, Nilssen
KJ (1995) Metomidate anaesthesia in Atlantic salmon, Salmo salar,
prevents plasma cortisol increase during stress. Aquaculture
134(1-2):155-168
NAL Call No. SH1A6
Atlantic salmon (S. salar) parr (58
g) in fresh water at 5.0°C and adult salmon (1130 g) in sea water at 7.7°C were
exposed to water containing different concentrations of metomidate in the range
1 to 10 mg/l. Metomidate was efficacious in inducing anaesthesia (hypnosis),
and efficacy increased with concentration over the interval tested. The
anaesthetic was more potent in the adult salmon acclimated to sea water than in
freshwater parr. Metomidate at 3 mg/l or higher completely prevented any plasma
cortisol increase after a handling stressor when stressor and anaesthetic were
applied concomitantly. The lack of a cortisol response seemed to be due to a
blockage at the level of the interrenal cell, since exogenous ACTH injected
intraperitoneally did not produce a cortisol increase in
metomidate-anaesthetized fish but did in those anaesthetized with MS-222. Blood
lactate levels and haematocrit increased in fish during metomidate anaesthesia.
Descriptors: fish culture, marine aquaculture, freshwater
aquaculture, biological stress, anaesthetics, haematology, anadromous species, Salmo
salar
ASFA; Copyright © 2003, FAO
Ortun J, Esteban MA, Meseguer J
(2002) Effects of phenoxyethanol on the innate immune system of gilthead
seabream (Sparus aurata L.) exposed to crowding stress. Veterinary
Immunology and Immunopathology. 89(1-2):29-36
NAL Call No.
SF757.2.V38
Phenoxyethanol is routinely used in seabream aquaculture to minimise fish stress
response despite the secondary negative effects which have been observed. In
this study, two different doses (60 and 200 mu l/l) of phenoxyethanol, sedative
and narcotic, were tested for their ability to reduce the stress caused in
gilthead seabream (Sparus aurata L.)
by crowding. Blood glucose and serum cortisol concentrations were measured as
stress indicators. In order to study the effects of the treatment on the innate
immune system of crowded specimens, two parameters of the innate immune
response, serum complement activity and phagocytosis, were assessed. The
results show that anaesthesia itself produced a stress response in the fish and
affected the immune system, although the effects were greater with the narcotic
dose. When the effects of anaesthesia on crowded fish were analysed, the
results pointed to a slight reduction in stress as a result of the sedative
dose of phenoxyethanol (lower increase in cortisol and lower reduction in
phagocytosis). However, additive negative effects were seen in crowded fish
when the narcotic dose of phenoxyethanol was used. Since the use of
phenoxyethanol is a common practice in aquaculture, the significance of the
results should be considered.
Descriptors: drugs, anaesthetics, biological stress,
immunity, stocking density, fish culture, complement, phagocytosis, anesthesia,
stress, glucose, hydrocortisone, aquaculture, fish immunity, phenoxyethanol, Sparus
aurata, phenoxyethanol, gilthead seabream, crowding
ASFA; Copyright © 2003, FAO
Ortuno J, Esteban MA, Meseguer J
(2002) Effects of four anaesthetics on the innate immune response of
gilthead seabream (Sparus aurata L.). Fish & Shellfish
Immunology. 12(1):49-59
NAL Call No. QL638.97.F55
Anaesthesia may depress the immune system in mammals, but there is no available
information on this topic in fish. In the present work, four anaesthetics that
are used in aquaculture, MS222 (0.19 mm), benzocaine (0.21 mm),
2-phenoxyethanol (1.6 mm) and quinaldine sulphate (0.083 mm), were tested in
order to observe their effects on the gilthead seabream (Sparus aurata L.) innate immune system. The results showed that the
four anaesthetics produced increased blood glucose levels after an hour. In
addition, benzocaine and 2-phenoxyethanol depressed complement activity and
phagocytosis, while MS222 and quinaldine sulphate did not. Some anaesthesia is
a common practice in aquaculture, the data obtained should be taken into
account to avoid possible immunodepression in farmed fish. Copyright 2002
Academic Press
Descriptors: immune response, anesthetics, glucose, blood
levels, phagocytosis, fish culture, cultured organisms, marine fish, fish
physiology, disease resistance, husbandry diseases, immunity, haematology,
anaesthesia, fish immunity, benzocaine, 2-phenoxyethanol, quinaldine sulfate,
Sparus aurata, gilthead seabream, immunodepression, blood glucose levels,
MS222
ASFA; Copyright © 2003, FAO
Ortuno J, Esteban MA, Meseguer J
(2002) Lack of effect of combining different stressors on innate immune
responses of seabream (Sparus aurata L.). Veterinary Immunology
and Immunopathology. 84(1-2):17-27
NAL Call No.
SF757.2.V38
A complex stressful event, which commonly occurs in modern aquacultural
practices, was broken down into factors that were analysed both individually and
jointly to assess their effect on two stress indicators (blood glucose and
serum cortisol levels) and two activities of the innate immune response (serum
complement and head-kidney leukocyte respiratory burst). For this, gilthead
seabream (Sparus aurata L.) specimens
were exposed to the following stressors: physical disturbance, crowding,
anaesthesia with 2-phenoxyethanol and air exposure. At 0, 1, 2, 3 and 4 days
post-stress, blood and serum samples were collected to measure glucose
concentration and cortisol and complement levels, respectively. Head-kidney
leukocytes were isolated and assayed to evaluate respiratory burst activity.
The results show that physical disturbance, crowding and anaesthesia produced
an occasional increase in glucose and cortisol concentrations. Crowding and
anaesthesia induced a depression in complement activity, while hypoxia by air
exposure caused a reduction in the respiratory burst. When all factors were
jointly applied both humoral and cellular defenses were compromised and
cortisol values remained high throughout the experimental period. Any long-term
effects of this abnormal serum cortisol levels on the immune system remain
unknown.
Descriptors: fish immunity, leukocytes, 2-phenoxyethanol,
glucose, cortisol, immunity, biological stress, stocking density, anaesthesia,
air exposure, defense mechanisms, handling, aquaculture techniques, Sparus
aurata, gilthead seabream, cortisol
ASFA; Copyright © 2003, FAO
Osanz Castan E, Esteban Alonso J,
del Nino Jesus A, Josa Serrano A, Espinosa Velazquez E (1993) Study on
quinaldine and 2-phenoxyethanol anaesthetics: Age and specie influence.
Actas del IV Congreso Nacional de Acuicultura., Centro de Investigaciones
Marinas, Pontevedra (Spain). pp. 737-742
We have studied the 2-phenoxyethanol and quinaldine effect on adult and fry
carp and the 2-phenoxyethanol effect on adult tench (Tinca tinca) and carp (Cyprinus
carpio). We have used different temperatures (10°C in winter and 20°C in
summer) and exposure time (1', 3', 5'. 7' and 10' minutes). Anaesthetic doses
employed are: 0.1, 0.2, 0.35 and 0.5 ml/l in 2-phenoxyethanol and 0.0125,
0.0250, 0.0375 and 0.05 ml/l in quinaldine.
Descriptors: fish culture, anaesthesia, serological
studies, blood, temperature, fry, Tinca tinca, Cyprinus carpio,
2-phenoxyethanol
ASFA; Copyright © 2003, FAO
Peres G, Roche H, Skrzynski J
(1989) The importance of hematological modifications of a biochemical nature
provoked by anesthesia in the fish. Bulletin de l'Academie Veterinaire
de France. 62(2):259-272
NAL Call No.
41.9 R24
Results of an experimentation using MS222 (Tricain Methane Sulfonate) in
the case of the sea fish Dicentrarchus
labrax and bibliographic data show that anaesthesia can induce more or less
great modifications of the biochemical hematology in function of utilization.
It may induce three kinds of consequence; a transient alteration of the
sanitary state; an interaction with some experimental process which might be
erroneous; an eventual incidence upon animal or human consumers which justifies
precautions concerning alimentary utilization.
Descriptors: haematology, biochemical composition, human
food, Dicentrarchus labrax, anaesthetics, MS222
ASFA; Copyright © 2003, FAO
Plumb JA, Schwedler TE, Limsuwan
C (1983) Experimental anesthesia of three species of freshwater fish with
etomidate. Progressive Fish Culturist. 45(1):30-33
NAL Call No. 157.5 P94
Etomidate (ethyl-1-methylbenzyl-imidazole-5-carboxylate) is an experimental
non-barbiturate hypnotic agent used intravenously for anesthetic induction in
humans and domestic or laboratory mammals. Three species of fish (golden
shiners, Notemigonus crysoleucas ;
striped bass, Morone saxatilis ; and
channel catfish, Ictalurus punctatus)
were anesthetized for up to 96 h with various concentrations of etomidate.
Mortality during the 48-h recovery period after anesthesia was low in all
species of fish. Two-year-old channel catfish withstood from 0.4 to 3.6 mg/L
for 80 min with low mortality at the higher concentrations, but anesthesia was
induced at 0.8 to 1.2 mg/L. Etomidate compared favorably to quinaldine and
tricaine methanesulfonate under the conditions tested.
Descriptors: anesthetics, experimental research, Notemigonus
crysoleucas, Morone saxatilis, Ictalurus punctatus, etomidate
ASFA; Copyright © 2003, FAO
Post G (1979) Carbonic acid anesthesia
for aquatic organisms. Progressive Fish Culturist. 41(3):142-144
NAL Call No. 157.5 P94
Carbonic acid in water is a safe,
inexpensive, effective, convenient, and easily obtainable anesthetic for fish and
other aquatic organisms. Carbonic acid is not a controlled substance in the
United States, and may not require US Food and Drug Administration
registration. Baths containing 150 to 600 mg/l carbonic acid (HSUB-2 COSUB-3)
will anaesthetize fish; lower concentrations anaesthetize more slowly and less
deeply, high concentrations act more rapidly and with greater sedation. The
length of time that fish or aquatic organisms can safely be held in
anaesthetizing baths depends on the carbonic acid concentration, and is longer
at low concentrations than at high concentrations. A convenient procedure for
developing known concentrations of carbonic acid in anaesthetizing baths
involves adding equal volumes of 6.75% (wt/vol) sodium bicarbonate solution and
3.95% (wt/vol) of concentrated (97-98%) sulphuric acid solution to a known
volume of water.
Descriptors: anaesthetics, carbonic acid
ASFA; Copyright © 2003, FAO
Prihoda J (1979) Experience with
use of propoxatein anaesthesia and transport of salmonids in Slovakian fishery
union centres. Biologizace a Chemizace Zivoicisne Vyroby Veterinaria.
15(3):283-288
NAL Call No.
SF1.B5
Following previous tests, anaesthetic
Propoxat of Janssen Co. was introduced into the fishery practice in all the
centres of Slovakian Fishery Union during the spawning period of breeding
fishes. To facilitate the application, 1% solution dosed 1 ml per 5L water
(solution 1:500,000) is used in practice. With view to the size of the
medicated basin, 30 to 50 fish are narcotized and after 2.5 min they are
transferred to a canvas cradle and spawned, while another batch of fish is put
into the basin. The solution is changed after 8 bathes. In the course of the
five-year usage no mortality was recorded owing to anaesthesia. In testing an
appropriate Propoxate concentration and the method of transporting fish,
hermatically closed polyethylene tanks containing 20 l of transporting solution
with Propoxate diluted 1:8,000,000 and filled with 16 kg of fish and 20 l of
oxygen approved their suitability for this purpose. No negative changes ere
found after a two hour transport, while 850 heads out of 5,000 control rainbow
trouts died during the classical transport in aerated water.
Descriptors: fish culture, spawning, anasthetics,
anaesthesia, induced breeding, Salmonidae,
Czechoslovakia
ASFA; Copyright © 2003, FAO
Prince A, Powell C (2000) Clove
Oil as an Anesthetic for Invasive Field Procedures on Adult Rainbow Trout. North
American Journal of Fisheries Management. 20(4):1029-1032
NAL Call No. SH219.N66
Clove oil has recently been proposed as an appropriate anesthetic for
researchers to use on food fish; however, its use in invasive procedures has
not yet been reported for salmonids. In a telemetric investigation, we found
the concentration of clove oil required for invasive procedures to be 30 mg/L,
which is 75% less than previously suggested dosages to achieve and maintain a
level of deep anesthesia in adult rainbow trout Oncorhynchus mykiss (N = 20). Mean time (" SE) to achieve level 4 anesthesia was 3.7 " 0.9 min. Average exposure time to the anesthetic
(surgery time) was 5.8 " 0.2 min, and average recovery time (the time required
to regain equilibrium and full swimming mobility) was 4.9 " 1.0 min. Lengthy recovery times (up to 18 min) were
observed; such times are typically reported for clove oil. Field investigators
should conduct preliminary trials to determine the appropriate concentration of
clove oil for their conditions, particularly if the application requires more
than 5 min of exposure to the anesthetic.
Descriptors: anaesthetics, bioassays, adults, Oncorhynchus
mykiss, clove oil, Rainbow trout
ASFA; Copyright © 2003, FAO
Puceat M, Garin D, Freminet A
(1989) Inhibitory effect of anaesthesia with 2-phenoxyethanol as compared to
MS222 on glucose release in isolated hepatocytes from rainbow trout (Salmo
gairdneri). Comparative Biochemistry and Physiology. 94A(2):221-224
NAL Call No. QP1 C6
Glucose production by freshly isolated hepatocytes from rainbow trout was
studied after anaesthesia of the animals with 2-phenoxy ethanol or tricaine
methanesulphonate. At the end of the procedure hepatic contents of glycogen,
glucose, lactate, ATP, ADP, AMP, were not significantly different between the
two treatments.
Descriptors: liver, cells, inhibitors, anaesthesia, Salmo
gairdneri, fish culture, fish physiology, biochemical phenomena, glucose,
hepatocytes, 2-phenoxyethanol, modulation, release
ASFA; Copyright © 2003, FAO
Rivera
Lopez H, Orbe Mendoza A, Ross LG (1991) Use of xylocaine, potentiated with sodium
bicarbonate, as an anaesthetic for fry and juveniles of acumara, Algansea lacustris Steindachner 1895,
from Lake Patzcuaro, Michoacan, Mexico. Aquaculture and Fisheries
Management. 22(1):15-18
The effectiveness of xylocaine anaesthesia, potentiated with sodium
bicarbonate, was investigated on the altiplano cyprinid, Algansea lacustris Steindachner. The drug gave excellent sedation,
handling and recovery and was effective at doses between 50 and 300 mg/l in 1
g/l sodium bicarbonate.
Descriptors: fish handling, anaesthesia, aquaculture
techniques, Algansea lacustris, fry, juveniles, Mexico, Michoacan,
Patzcuaro Lake, drugs, fish culture, xylocaine, sodium bicarbonate
ASFA; Copyright © 2003, FAO
Ross LG, Ross B (1999) Anaesthetic
and Sedative Techniques for Aquatic Animals. Blackwell
Science,
NAL Call No. SH156.9 R67
1999
The handling of fish and other aquatic animals in an out of their natural
environment is always difficult. Their struggling has strong effects on their
physiology and behaviour and the animals can easily be damaged. Anaesthesia and
sedation are therefore essential techniques in fisheries management and
aquaculture to minimize stress and physical damage caused by crowding, capture,
handling and release. This new text fulfils a proven need for an illustrated,
practical guide for workers in aquaculture and fisheries research and
management. Based on first-hand experience, the text covers fish, amphibian and
reptiles and includes a glossary of drugs, an explanation of major technical
terms and an index for ease of reference.
Descriptors: disease control, aquaculture, fish diseases,
anaesthetics, fish handling
ASFA; Copyright © 2003, FAO
Ross LG, Ross B (1984) Anaesthetic
and Sedative Techniques for Fish. Institute of Aquaculture, Stirling
(UK), 42 pp.
NAL Call No.
SH156.9 R6 1984
The purpose of this handbook is to draw together the available information
on sedation and anaesthesia of fishes. Both temperate and tropical freshwater
species are considered as well as sedation in sea water. While sedation is a a
routine and essentially simple procedure it can also be mismanaged. The overall
intention is therefore to produce an illustrated, practical guide for workers both
in aquaculture and in research.
Descriptors: anaesthesia, anesthetics, fish, Pisces
ASFA; Copyright © 2003, FAO
Ross RM, Backman TWH, Bennett RM
(1993) Evaluation of the anesthetic metomidate for the handling and transport
of juvenile American shad. Progressive Fish Culturist 55(4):236-243
NAL Call No. 157.5 P94
Juvenile American shad (Alosa sapidissima)
were exposed to three levels of metomidate (0.0 = control, 0.5, and 1.0 mg/L)
and three types of sedation or handling (none, sedation only, and handling
after sedation) to determine the efficacy and safety of the drug for use in
transport and handling of this species. Mean sedation times were 9 and 3 min,
and mean recovery times were 6 and 7 min, respectively, for the 0.5- and
1.0-mg/L concentrations of metomidate. For fish exposed to 1.0 mg metomidate/L,
normal swimming behavior was delayed as long as 4 h after fish were placed in
drug-free water. Aggregating and parallel orientation behaviors, precursors of
normal schooling, were significantly reduced at the highest drug level for 1 h,
but not for 24 h, after recovery. No posttreatment difference in behavior was
observed as a result of handling fish (removal from water, weighing, and
measuring) under sedation. Long-term (50-d) survival was not affected by drug
concentration in one experiment and was improved by use of the anesthetic in a
second study. Metomidate appears to be useful and safe for the transportation
and handling of juvenile American shad.
Descriptors: anaesthetics, handling, transport, juveniles,
Alosa sapidissima, metomidate
ASFA; Copyright © 2003, FAO
Roubach R, De Carvalho Gomes L,
Val AL (2001) Safest level of tricaine methanesulfonate (MS-222) to induce
anesthesia in juveniles of matrinxa, Brycon cephalus. Acta
Amazonica. 31(1):159-163
NAL Call No.
QH7.A2
The use of MS-222 as an anesthetic for matrinxa juveniles was investigated. At
dosage of 100 mg/L or lower fish did not achieve a complete anesthesia state.
At 150 mg/L, MS-222 induced anesthesia within 36 seconds and recovered from a
10 minutes period of anesthesia within 5.2 min. Higher concentrations (200, 250
and 300 mg/L) anesthetized fish in lesser times, with the offset of mortality
(16.7 and 33.3%) at the 200 and 300 mg/L MS-222 doses, respectively. The only
significant differences observed in the hematological parameters, was for the
glucose values in fish anesthetized with 250 and 300 mg/L. From the results,
the recommended dose of MS-222 for handling matrinxa juveniles is 150 mg/L.
Descriptors: anaesthetics, anaesthesia, juveniles, fish
handling, Brycon cephalus, tricaine methanesulfonate
ASFA; Copyright © 2003, FAO
Sado EK (1985) Influence of the
anaesthetic quinaldine on some tilapias. Aquaculture.
46(1):55-62
NAL Call No. SH1A6
Three species of tilapia, Sarotherodon melanotheron and Sarotherodon
guincensis from brackish water and Oreochromis
(Sarotherodon) noloticus from fresh water, were exposed to different
concentrations of the anaesthetic quinaldine to determine the safe level for
handling and transportation of these species. Dosages of quinaldine for
handling fish for experimental work and for transportation are given with the
proviso that the anaesthetization is carried out under conditions of salinity
and temperature which are suitable for acclimation.
Descriptors: anesthetics, live storage, Sarotherodon
melanotheron, Sarotherodon guincensis, Oreochromis niloticus, environmental
factors, quinaldine
ASFA; Copyright © 2003, FAO
Sandodden R, Finstad B, Iversen M
(2001) Transport stress in Atlantic salmon (Salmo salar L.):
anaesthesia and recovery. Aquaculture Research. 32(2):87-90
NAL Call No. SH1 F8
The effects of metomidate anaesthesia on levels of plasma cortisol, glucose,
haematocrit and chloride in Atlantic salmon (Salmo salar L.), after a 2-h transport and during a 48-h recovery
period were investigated. The use of metomidate anaesthesia during transport
led to a reduced release of cortisol and significantly lower levels of plasma
cortisol after a 48-h recovery period. Plasma glucose did not return to basal
level after a 48-h recovery period, indicating that even longer recovery may be
needed for the fish to return to a pre-stress state. The results show that
metomidate anaesthesia combined with a recovery period lessens the stress
burden imposed by hauling and transport.
Descriptors: biological stress, anaesthesia,
transportation, fish culture, fish physiology, serological studies, Salmo
salar, Atlantic salmon
ASFA; Copyright © 2003, FAO
Sayer MDJ, Cameron KS, Wilkinson G
(1994) Fish species found in the rocky sublittoral during winter months as
revealed by the underwater application of the anaesthetic quinaldine. Journal of Fish Biology. 44(2):351-353
NAL Call No. QL614 J68
Using the anaesthetic quinaldine applied underwater, fish species not normally
observed in the Scottish rocky sublittoral during the winter months have been
recorded, and are listed.
Descriptors: check lists, distribution records, marine
fish, rocky shores, littoral zone, winter, ANE, British Isles, Scotland,
anaesthetics, Pisces, methodology, species composition, coastal waters
ASFA; Copyright © 2003, FAO
Schramm HL Jr, Black DJ (1984) Anesthesia
and surgical procedures for implanting radio transmitters into grass carp.
Progressive Fish Culturist. 46(3):185-190
NAL Call No. 157.5 P94
The authors investigated anesthetic and surgical procedures useful for
implanting radio transmitters into the body cavity of 1.6-3.7 kg (3.5-8.2 lb)
grass carp (Ctenopharyngodon idella).
Quinaldine was an effective anesthetic for fish at water temperatures < 26oC
(79 oF); however, it was lethal at concentration necessary to induce
and maintain sufficient anesthesia for surgery at water temperatures > 29
oC (84 oF). MS-222 was a suitable anesthetic at all water
temperatures. Implantation of radio tags through midventral incisions was
preferable to implantation through lateral incisions, because there was no
danger of puncturing the ovaries of female fish and the operation was more
easily performed. Surgical procedures are presented.
Descriptors: Ctenopharyngodon idella, biotelemetry,
activity patterns, local movements, anaesthesia, surgical implantation
ASFA; Copyright © 2003, FAO
Sekizawa Y, Umemura K, Shimura M,
Suzuki A, Kikuchi T (1975) Residue analyses on 2-amino-4-phenylthiazole, a
piscine anesthetic, in fishes 1. A model radiotracer experiment with medaka.
Bulletin of the Japanese Society of Scientific Fisheries. 41(4):449-458
NAL Call No. 414.9 J274
Residue analyses on Super(3)H.2-amino-4-phenylthiazole were performed in medaka
(killifish, Oryzias latipes).This
fish was selected because of its ready application in radiotracer experiments
and its marked and unique ability to detoxify the piscine anesthetic,
2-amino-4-phenylthiazole to its N-hexuronyl conjugate. The absorption/excretion
balance showed an average 102% recovery indicating that the excretion treatment
effectively exhausted the residue from medaka, The 1/2 life for retention of
this compd and its N-hexuronyl conjugate in the body of the fish was approx
12-15 hr. The fates of both the anesthetic and its N-hexuronyl conjugate in the
fish body were determined separately in a time course survey using TLC
radiography. plots for the excretion of the major product, i.e. the conjugate,
and the anesthetic per se produced 2 crossing exponential curves suggesting
that there are 2 compartmentations of both the conjugate and the anesthetic per
se in medaka. A mathematical simulation of the residue was also described.
Descriptors: anaesthesia, bioaccumulation, chemical
analysis, metabolism, Oryzias latipes
ASFA; Copyright © 2003, FAO
Comparative impact of chloral hydrate anaesthesia on the metabolic rate of
Indian major carp Labeo rohita and
larvivorous fish Poecilia reticulata
was assessed. Observation on the oxygen consumption rate (OCR) revealed that in
common guppies OCR was substantially low (1.105 and 1.097 mg/g/hr) at 0.1 and
0.25 g/l concentrations of chloral hydrate as against OCR of 1.487 mg/g/hr in
the control. Fry L. rohita in group
showed lower metabolic rates in the control as well as treated conditions as
compared to the individuals of this fish. This may be due to sympathetic
psychophysiological reflex of grouped fish. Higher dose of chloral hydrate
(0.25 g/l) also caused higher OCR probably due to distress. Application of
chloral hydrate also favoured lesser release of metabolic wastes (ammonia and
carbon dioxide). There was significant positive correlation between time and
oxygen consumption, whereas, for time and OCR this relationship was negative.
Regression of chloral hydrate doses for OCR and time has also been calculated.
Descriptors: anaesthetics, oxygen consumption, freshwater
fish, Labeo rohita, Poecilia reticulata,
ASFA; Copyright © 2003, FAO
Shimura M, Sekizawa Y (1975) Residue
analyses on 2-amino-4-phenylthiazole, a piscine anesthetic, in fishes 2.
Resolution on chemical structure of the metabolite in medaka. Bulletin
of the Japanese Society of Scientific Fisheries. 41(5):529-534
NAL Call No. 414.9 J274
The metabolite of 2-amino-4-phenylthiazole in medaka
(killifish, Oryzias latipes) was
isolated as white crystals from environmental water in which the fish were
treated with the anesthetic. Acid hydrolysis of the metabolite gave crystallize
2-amino-4-phenylthiazole and a spot on TLC coinciding with that of D-glucuronic
acid. Gas chromatography of the metabolite after methanolysis and
trimethylsilylation gave peaks coinciding with those of authentic D-glucuronic
acid and 2-amino-4- phenylthiazole. The colour reaction of the metabolite
suggested that the conjugation site of these 2 constituents was the primary
amino group of the thiazole ring and the aldehyde group of glucuronic acid.
Reaction of D-glucuronic acid and 2-amino-4-pheylthiazole in 50% acetone under
reflux resulted in the formation of a product identical with the naturally
occurring metabolite. The NMR spectrum of the metabolite in D Sub(2)O revealed
that the structure of the sugar moiety was {beta}-D-glucopyranosiduronic acid.
Thus it was concluded that the chemical structure of the metabolite in medaka
should be 2-amino-4- phenylthiazole 2-N-{beta}-D-glucopyranosiduronic acid.
Descriptors: anaesthesia, metabolites, Oryzias latipes,
chemical structure
ASFA; Copyright © 2003, FAO
Sijm DTHM, Bol J, Seinen W,
Opperhuizen A. (1993) Ethyl
m-aminobenzoate methanesulfonate dependent and carrier dependent
pharmacokinetics of extremely lipophilic compounds in rainbow trout. Archives
of Environmental Contamination and Toxicology. 25(1):102-109
NAL Call No. TD172 A7
Significant differences were found in both uptake and pharmacokinetics in fish
(Oncorhynchus mykiss) when six
lipophilic compounds were dosed by gavage in either an oil or an gelatin
carrier. Pharmacokinetics were also different when fish were anaesthetized with
ethyl m-aminobenzoate methanesulfonate (MS-222) before dosing. The highest
uptake percentages, uptake rates and concentrations of the compounds were found
in the fish which were given the gelatin carrier only. MS-222 decreased the
uptake of the compounds. Absorption of the compounds from oil was lower than
from gelatin. In addition, absorption from oil continued for 21 d, which lasted
longer than from gelatin.
Descriptors: pharmacokinetics, lipids, chemicals,
bioaccumulation, absorption, oil, gelatin, liver, diets, Oncorhynchus mykiss, tricaine, oils, chemical kinetics,
anaesthesia, correlation, absorption coefficient, chlorinated hydrocarbons,
freshwater fish, anadromous species
ASFA; Copyright © 2003, FAO
Siwicki A
(1984) New anaesthetic for fish. Aquaculture.
38(2):171-176
NAL Call No. SH1A6
Anaesthetics are needed when handling fish, especially
during tagging. However, most anaesthetics applied at present have a strong
toxic effect on fish. For this reason it is only permissible to keep fish
anaesthetized for a short time. A new anaesthetic “Propanidid” has been
successfully tested which allows fish to be anaesthetized for up to 1 h. It can
be applied as an intraperitoneal injection and as a bath solution. The required
disappearance of sense perception and motor reflexes in the fish can be obtained
in 2-4 min. Low toxicity of the pharmacological preparation has been proved
according to a full set of the clinical, toxicological, haematological and
biochemical criteria. Clinical tests were carried out with salmonids mainly.
The new compound belongs to a group of anaesthetics which are used in human
medicine; thus, it is considered to be harmless for man as an operator and a
consumer.
Descriptors: fish culture, aquaculture techniques,
anesthetics, fish handling, Propanidid
ASFA; Copyright © 2003, FAO
Siwicki A, Jeney Z (1986) Surgical
intervention in wels (Silurus glanis
L.) during artificial propagation. Aquacultura Hungarica Szarvas [Aquacult.
Hung.]. 5:55-58
NAL Call No.
SH101 H8A68
Experience obtained during surgical intervention in artificial propagation of
Descriptors: fish culture, induced breeding, anaesthesia, Silurus glanis, testes, surgery
ASFA; Copyright © 2003, FAO
Smit GL, Hattingh J (1979) Anaesthetic
potency of MS 222 and neutralized MS 222 as studied in three freshwater fish
species. Comparative Biochemistry and Physiology. 62C(2):237-241
NAL Call No.
QP901 C6
The effects of several different concentrations of MS 222 and neutralized
MS 222 on aquarium water quality and anesthetic potency were investigated in Cyprinus carpio, Sarotherodon mossambicus and Salmo
gairdneri. MS 222 caused a decrease in pH and bicarbonate alkalinity with a
corresponding increase in pCO2 of the aquarium water. Conductivity
was also increased. Neutralized MS 222 prevented, apart from an increase in
conductivity, such effects. Increasing the concentration of MS 222 in the
aquarium water resulted in a decrease in anaesthetic induction times in all
three fish species studied. Neutralized MS 222 induced anaesthesia reduced
induction times further and also increased recovery times from anaesthesia. The
amount of free MS 222 in the blood of the three fish species studied did not
differ significantly from each other per MS 222 concentration employed and
showed an increase with increased concentrations of MS 222. With neutralized MS
222 the concentration of free MS 222 in the blood of the three fish species was
generally higher. Neutralized MS 222 thus resulted in a deeper, more consistent
anaesthesia, thereby indicating a safer, more effective, longer-acting
anaesthetic.
Descriptors: anaesthetics,
Pisces, aquaria, Cyprinus carpio,
Sarotherodon mossambicus, Salmo gairdneri, water quality
ASFA; Copyright © 2003, FAO
Smit GL, Hattingh J, Burger AP
(1979) Haematological assessment of the effects of the anaesthetic MS 222 in
natural and neutralized form in three freshwater fish species: haemoglobin
electrophoresis, ATP levels and corpuscular fragility curves. Journal of
Fish Biology. 15(6):655-663
NAL Call No. QL614 J68
The effects of MS 222 and neutralized MS 222 anaesthesia on haemoglobin
electrophoresis, erythrocyte adenosine triphosphate (ATP) levels and
corpuscular fragility curves were studied in Cyprinus carpio, Sarotherodon
mossambicus and Salmo gairdneri.
Haemoglobin electrophoresis showed no significant intra-species differences in
the percentage composition of the various fractions for any concentration of MS
222 and neutralized MS 222 used. Significant interspecies differences were,
however, still observed. ATP levels showed intra- and interspecies differences
ascribed to the response of the fish species to MS 222-induced stress and not
to actual changes in erythrocyte ATP concentrations. Differences were also
observed in corpuscular fragility curves for all three species when using MS
222 or neutralized MS 222 compared to curves obtained without the use of the
anaesthetic, but the mechanisms involved are not clear.
Descriptors: haematology, anaesthetics, erythrocytes,
haemoglobin, Cyprinus carpio, Sarotherodon mossambicus, Salmo gairdneri, Cyprinidae, Salmonidae,
Cichlidae, Pisces, ATP, ethyl <m->aminobenzoate, effects on, fish
ASFA; Copyright © 2003, FAO
Smit GL, Hattingh J, Burger AP
(1979) Haematological assessment of the effects of the anaesthetic MS 222 in
natural and neutralized form in three freshwater fish species: interspecies
differences. Journal of Fish
Biology. 15(6):633-643
NAL Call No. QL614 J68
Interspecies haematological differences to MS 222 and neutralized MS 222
anaesthesia were investigated in Sarotherodon
mossambicus, Cyprinus carpio and Salmo gairdneri acclimated under
identical laboratory conditions. Anaesthesia with MS 222 resulted in a
‘chemical stress’ in all fish, as was evident from changes in the
haematological profiles of the animals. Such species variations in the
haematology persisted throughout the whole experiment protocol which employed
different concentrations of the anaesthetic. The use of neutralized MS 222,
whereby aquarium water quality remained unchanged, improved the haematological
profile. Possible reasons for the interspecies differences observed are
discussed.
Descriptors: haematology, anaesthetics, Cyprinus carpio, Salmo gairdneri,
Sarotherodon mossambicus, Cyprinidae, Salmonidae, Cichlidae, Pisces, fish
culture, ethyl m-aminobenzoate, effects on, sedatives, local anaesthetics
ASFA; Copyright © 2003, FAO
Smit GL, Hattingh J, Burger AP
(1979) Haematological assessment of the effects of the anaesthetic MS 222 in
natural and neutralized form in three freshwater fish species: intraspecies
differences. Journal of Fish
Biology. 15(6):645-653
NAL Call No. QL614 J68
The effects of different concentrations of natural MS 222 and neutralized MS
222 were studied on the haematology of Cyprinus
carpio, Sarotherodon mossambicus
and Salmo gairdneri. As judged from
the results, MS 222, which is acidic in nature, produced haemoconcentration
effects in all species studied, being least in the trout followed by carp and
Sarotherodon mossambicus. These differences are ascribed to acid-base regulatory
functions and metabolic activities of the fish species investigated. The use of
neutralized MS 222 improved the haematological profiles markedly and resulted
in stabilization of acid-base parameters and red blood cells sizes and numbers.
Haemoconcentration effects, however, still persisted. Trout were found to be
more susceptible to the stress of MS 222 anaesthesia than carp and Sarotherodon mossambicus.
Descriptors: haematology, anaesthetics, Sarotherodon mossambicus, Salmo gairdneri, Cyprinus carpio, Cichlidae, Salmonidae, Cyprinidae,
ethyl-m-aminobenzoate, neutralization, effects on, sedatives, local
anaesthetics
ASFA; Copyright © 2003, FAO
Smit GL, Schoonbee HJ, Barham WT
(1979) Some effects of the anaesthetic MS 222 on fresh water. South
African Journal of Science 73(11):351-352
NAL Call No. 515 SO84
Previous research has shown that MS 222 (tricaine
methanesulphonate) added to water to produce anaesthesia in fish has a variable
effect. The possibility that this may be caused by differences in mineral
content was investigated in the study reported. MS 222 was added to tapwater,
deionized water, distilled water, borehole water and water from a dolomite
spring and the pH, pCO2, pO2, orthophosphate content and
conductivity was measured at various times up to 1 1/2 h. Results show that
because of the changes in pH and conductivity induced in deionized and
distilled water, these should not be used in laboratory experiments in the presence
of MS 222. The changes, which occur within 2 minutes and continue for at least
30 minutes, could significantly affect the physiology of target fish. However,
water with a good buffering ability, such as that from the dolomite spring, was
relatively unaffected by MS 222. It is concluded that water with a high mineral
content is best suited for the laboratory use of MS 222.
Descriptors: anaesthetics, environmental effects, Pisces,
physiology
ASFA; Copyright © 2003, FAO
Smith DA, Smith SA, Holladay SD
(1999) Effect of previous exposure to tricaine methanesulfonate on time to
anesthesia in hybrid tilapias. Journal of Aquatic Animal Health. 11(2):183-186
NAL Call No. SH171.J68
Tricaine methanesulfonate (MS-222) is one
of the most broadly used anesthetic and tranquilizing agents for poikilotherms,
and it is currently the only anesthetic approved for use with food fish. To
test the preliminary observation in tilapias Oreochromis sp. previously exposed to anesthetizing doses of MS-222
that time to anesthesia was shorter on subsequent exposures, hybrids of white
tilapias (Nile tilapia O. niloticus x
blue tilapia O. aureus) crossed with
Mozambique tilapias O. mossambicus
were anesthetized with MS-222. Time to anesthesia was recorded at this initial
drug exposure and weekly for 6 weeks after initial exposure. Fish previously
exposed to MS-222 did not display a significantly reduced time to anesthesia on
the second exposure but did display significant reductions at the third
exposure and thereafter. These preliminary results suggest that tilapias do not
respond to MS-222 with the typical enzyme induction-mediated tolerance reaction
commonly seen with anesthetic chemicals in mammals.
Descriptors: hybrids, anaesthesia,
tolerance, enzymes, oreochromis, tricaine methanesulfonate
ASFA; Copyright © 2003, FAO
Smith MFL (1992) Capture and
transportation of elasmobranchs, with emphasis on the grey nurse shark (Carcharias taurus). Sharks:
Biology And Fisheries., Australian Journal of Marine and Freshwater
Research. 43(1):325-343
NAL Call No.
QL638.9 I57 1991
Physiological changes manifest during the
capture and transportation of elasmobranchs are discussed. Reference is made to
the general adaptation syndrome and to mechanisms of change in concentrations
of blood glucose, blood acid and serum electrolyte. Methods of alleviating
these profound changes are suggested, with emphasis on the capture and
transportation of the grey nurse shark (Carcharias
taurus). The hoop method is shown to be a convenient technique for
capturing wild grey sharks, and a combination of ketamine hydrochloride and
xylazine hydrochloride, with antagonism by yohimbine hydrochloride, is found to
provide an acceptable regime of transportation anesthesia. Intravenous
administration of sodium bicarbonate and glucose as well as oxygenation of
water in the life-support system are shown to be valuable methods of
alleviating stress-induced blood acidosis.
Descriptors: captivity, transportation, Carcharias taurus, biological stress,
fish culture, fish handling, anaesthesia
ASFA; Copyright © 2003, FAO
Soivio A, Hughes GM (1978) Circulatory
changes in secondary lamellae of Salmo
gairdneri gills in hypoxia and anaesthesia. Annales Zoologici
Fennici. 15(3):221-225
NAL Call No. 410 AN712
The secondary lamellae of rainbow trout gills from hypoxic and anaesthetized
fish were analysed stereologically for the blood volume and haematocrit value.
In hypoxia the gills undergo vasodilatation, which is accompanied by slight
haemodilution. When oxygen is available the situation changes; there is a
tendency to vasoconstriction, but a continuing decrease in the haematocrit
value. In anaesthesia the vasodilatation seen in the secondary lamellae is
combined with haemoconcentration. During recovery vasodilatation continues,
while the haematocrit value falls to below that of controls. Explanations for
these observations are offered in terms of the circulation through the gills.
Descriptors: respiratory organs, blood circulation, Salmo gairdneri, physiology,
anaesthesia, hypoxia, blood
ASFA; Copyright © 2003, FAO
Soivio A, Nyholm K, Huhti M (1977)
Effects of anaesthesia with MS 222, neutralized MS 222 and benzocaine on the
blood constituents of rainbow trout, Salmo
gairdneri. Journal of Fish
Biology. 10(1):91-101
NAL Call No. QL614 J68
Rainbow trout (S.gairdneri
Richardson) were subjected to 15 min anaesthesia with unbuffered MS 222,
neutralized MS 222 and benzocaine with and without physical stress. Blood
samples were taken through cannulae inserted into the doral aorta. The Hct
values and Hb concns increased with all the anaesthetics, which also caused
swelling of erythrocytes. The initial values were restored within 4-12 h of
recovery. Each anaesthetic elevated the blood lactate concn, but the initial
level was regained within 12 hr. The blood glucose level decreased the most
during anaesthesia with unbuffered MS 222, but the initial level was rapidly
restored. Benzocaine caused the least hypoglycaemia during anaesthesia, but the
subsequent hyperglycaemia, as in the fish anaesthetized with neutralized MS
222, lasted 24 hr. Neutralized MS 222 and benzocaine elevated the plasma K+
concn more rapidly than unbuffered MS 222. The initial levels were regained in
4 days. All anaesthetics raised the Mg++ concn. The pO2
in the dorsal aorta decreased during anaesthesia with unbuffered MS 222 by
about 85 mmHg, while the pCO2 increased by about 1.5 mmHg. Their
initial levels were regained within 20 min. During anaesthesia the pH value
decreased by 0.3 units and returned to the initial value within 2-4 hr of
recovery. MS 222 seemed to be an asphyxiant.
Descriptors: anaesthesia, blood, biological stress,
hypoxia, Oncorhynchus mykiss
ASFA; Copyright © 2003, FAO
Soto C (1995) Clove oil: A fish
anaesthetic. Window Newsletter.
ISSN: 1024-4158
In experiments in
Descriptors: botanical resources, drugs, anaesthetics,
anaesthesia, fish culture, aquaculture, Siganus
lineatus, Eugenia aromatica, ISEW,
ASFA; Copyright © 2003, FAO
Soto CG, Burhanuddin (1995) Clove
oil as a fish anaesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture. 136(1-2):149-152
NAL Call No. SH1A6
The successful use of clove oil as a fish anaesthetic
is described. Juvenile rabbitfish, Siganus
lineatus, from the same cohort ranging in size from 5 to 23 cm in total
length were anaesthetized, and their length and weight were recorded on three
separate occasions. Fish fed shortly afterward, and no mortality was observed.
Clove oil appears to be highly effective as a fish anaesthetic with potentially
few or no side effects. In Indonesia, its use is advantageous because it is
locally produced and inexpensive.
Descriptors: anaesthetics, Siganus lineatus, aquaculture techniques, Indonesia, fish culture,
clove oil
ASFA; Copyright © 2003, FAO
Spotte S, Bubucis PM, Anderson G
(1991) Plasma cortisol response of seawater-adapted mummichogs (Fundulus heteroclitus) during deep
MS-222 anesthesia. Zoo Biology 10(1):75-79
NAL Call No. QL77.5.Z6
Anesthetics are used to reduce stress in fishes during handling and transfer.
However, deep anesthesia of seawater-adapted mummichogs (Fundulus heteroclitus) results in a time-related increase in plasma
cortisol, indicating a primary (neuroendocrine) stress response. Groups of
seven fish were bled within 1 to 12 min of exposure to the anesthetic MS-222.
Plasma cortisol rose more rapidly in fish removed from the MS-222 solution
immediately after 1 min and held between wet paper towels than in fish that
remained immersed. The difference between methods was significant (P <
0.001) with variation restricted to the later sampling periods. Differences
were not significant in fish sampled immediately after 1 min.
Descriptors: serological studies, steroids, biological
stress, anaesthetics, Fundulus heteroclitus, fish physiology, MS-222
ASFA; Copyright © 2003, FAO
Stabell OB, Aanesen RT, Eilertsen
HC (1999) Toxic peculiarities of the marine alga Phaeocystis pouchetii detected by in vivo and in vitro bioassay
methods. Aquatic Toxicology. 44(4):279-288
NAL Call No. QH541.5.W3A6
The marine alga Phaeocystis pouchetii
has recently been shown to display toxic properties towards fish (i.e. cod, Gadus morhua) larvae. The assumed toxic
principle of this prymnesiophyte was extracted from Phaeocystis cultures by filtering and solid phase sorbent
techniques. Toxicity testing was carried out by in vitro and in vivo bioassay
methods based on blood haemolysis and injection into flies. The active material
from the Phaeocystis cultures was
found within a chemical fraction previously established for the separation of Chrysochromulina sp. and Prymnesium sp. toxins. The presence of
active material was also found in filtered seawater collected during a Phaeocystis bloom, confirming that Phaeocystis releases the active material
into the natural environment. Haemolytic activity was almost absent in the
material tested, demonstrating that the toxic principle in Phaeocystis is different from that described for other
prymnesiophytes. By the fly bioassay method, on the other hand, a rapid
response to injected material was obtained, resulting in a high proportion of
apparently ‘dead’ flies registered within 1 h. The time scale of response in
flies coincided with that previously reported for haemolytic toxins of Prymnesium parvum. However, an
unexpected response was observed with the Phaeocystis
material, since some of the flies that were assumed dead regained motility
within a 4-h period of monitoring, the portion of awakened flies being
inversely related to dose injected. Regained motility was also found with
injected material from filtered natural seawater. Accordingly, the proposed
toxins released by Phaeocystis appear
to be compounds that hold anaesthetic properties, possibly expressing toxic
effects when presented in surplus doses. These findings suggest that Phaeocystis may primarily be harmful to
fish larvae following ingestion.
Descriptors: biological poisons, metabolites,
anaesthetics, bioassays, seaweeds, fish larvae, algae, toxins, toxicity
testing, hemolysis, algal blooms, bioassay, analytical methods, toxicity,
marine organisms, larvae, ingestion, aquatic organisms, Pisces, Phaeocystis pouchetti, Phaeocystis
pouchetii, Gadus morhua, Atlantic cod
ASFA; Copyright © 2003, FAO
Stech L,
Paddlefish eggs when ovulated are discharged into the body cavity and exit
through one of two ovarian funnels. Two methods are currently practiced in
artificial propagation to remove the eggs from an ovulating fish:
hand-stripping or caesarean section. Hand stripping is labor intensive often
taking 8 to 10 hours to remove the eggs. Caesarean section is a quick method to
remove the eggs in 10 to 15 minutes, but often the broodstock do not survive. Because of complications with both methods for egg removal, a new
experimental procedure which in minimally invasive was developed for quick
removal of eggs from an ovulated paddlefish. Ovulated female was
anesthetized with 80 mg/l of MS-222 and placed ventral side up on a stretcher.
A finger was inserted into the gonopore to enlarge the opening. A scalpel (no.
11 blade) was carefully inserted into the opening and a 1 to 2 cm incision was
made through the oviduct (Mullerian duct) wall. After removal of the scalpel,
the incision was probed with an inserted finger to insure that the opening
permitted free copyright flow of eggs. The fish was inverted and pressure
placed on the abdomen region by two personnel so the eggs flowed out the
gonopore. Ten fish were ovulated using this method and time required to remove
the eggs was 7 to 10 min/fish. Three of the fish were sacrificed to observe the
effect from this method. Only a small quantity of eggs were
retained in these fish. The other fish were stocked in ponds without any ill
effects and will be used as potential broodstock in the future.
Descriptors: fish eggs, methodology, females, seed
collection, ovulation, brood stocks, anaesthesia, aquaculture techniques, Polyodon spathula,
ASFA; Copyright © 2003, FAO
Stehly GR, Gingerich WH (1999) Evaluation
of AQUI-S (efficacy and minimum toxic concentration) as a fish
anaesthetic/sedative for public aquaculture in the
NAL Call No. SH1 F8
A preliminary evaluation of efficacy and minimum toxic concentration of AQUI-S,
a fish anaesthetic/sedative, was determined in two size classes of six species
of fish important to US public aquaculture (bluegill, channel catfish, lake
trout, rainbow trout, walleye and yellow perch). In addition, efficacy and
minimum toxic concentration were determined in juvenile-young adult (fish aged
1 year or older) rainbow trout acclimated to water at 7°C, 12°C and 17°C.
Testing concentrations were based on determinations made with range-finding
studies for both efficacy and minimum toxic concentration. Most of the tested
juvenile-young adult fish species were induced in 3 min or less at a nominal
AQUI-S concentration of 20 mg/L. In juvenile-young adult fish, the minimum
toxic concentration was at least 2.5 times the selected efficacious
concentration. Three out of five species of fry fingerlings (1.25-12.5 cm in
length and < 1 year old) were induced in less than or equal to 4.1 min at a
nominal concentration of 20 mg/L AQUI-S, with the other two species requiring
nominal concentrations of 25 and 35 mg/L for similar times of induction.
Recovery times were less than or equal to 7.3 min for all species in the two
size classes. In fry-fingerlings, the minimum toxic concentration was at least
1.4 times the selected efficacious concentration. There appeared to be little
relationship between size of fish and concentrations or times to induction,
recovery times and minimum toxic concentration. The times required for
induction and for recovery were increased in rainbow trout as the acclimation
temperature was reduced.
Descriptors: anesthetics,
sedatives, fish culture, aquaculture techniques, anaesthesia, public health,
toxicology, toxicity tests, Stizostedion
vitreum vitreum, Perca flavescens, Oncorhynchus mykiss, Ictalurus punctatus,
Salvelinus namaycush, Lepomis macrochirus, USA, bluegill, rainbow trout,
channel catfish, graceful catfish, lake trout, walleye, yellow perch
ASFA; Copyright © 2003, FAO
Stone DS, Tostin N (1999) Clove
bud oil a big yawn for silver perch. Fisheries NSW, Sydney. 2(4):19
Anaesthetics are used in aquaculture to minimize stress and damage to fish
during harvesting, grading, transportation, spawning induction and handling.
AQUI-S clove bud oil and benzocaine are among some of the anaesthetics
currently being considered for use in the silver perch industry. At present,
AQUI-S is the only anaesthetic registered with the National Registration
Authority for use for food fish in Australia. The required concentration of
each of several anaesthetics was evaluated to induce suitable levels of
anaesthesia for the handling of silver perch (Bidyanus bidyanus) during harvesting or spawning induction. The
study determined effective concentrations for three anaesthetics for harvesting
and spawning induction for silver perch. All anaesthetics were reliable and
easy to use, but clove bud oil was the most efficient and economical. For clove
bud oil to be used as an anaesthetic for silver perch in the future, there is
an urgent need for its registration with the National Registration Authority.
Descriptors: anaesthetics, fish handling, harvesting,
anaesthesia, aquaculture techniques, fish culture, biological stress, food
fish, Bidyanus bidyanus
ASFA; Copyright © 2003, FAO
Strange RJ, Schreck CB (1978) Anesthetic
and handling stress on survival and cortisol concentration in yearling chinook
salmon (Oncorhynchus tshawytscha).
Journal of the Fisheries Research Board of Canada 35(3):345-349
NAL Call No: 442.9 C16J
Brief anesthetization with 50 mg/L buffered MS-222
RegTM (ethyl m-aminobenzoate methanesulfonate) of yearling chinook salmon
during mild handling caused no change in plasma cortisol concentrations
compared with levels in non-anesthetized fish. Prolonged exposure (180 min) to
a depressing dose of buffered MS-222 RegTM (25 mg/L) elevated cortisol more
than an immobilizing dose (50 mg/L), while 100 mg/L was lethal within 30 min.
Fish anesthetized (50 mg/L MS-222 RegTM) during a severe 30-min handling stress
had significantly lower mortality than controls to a second handling stress
applied when the fish were no longer anesthetized. Anesthetization during the
first stressor also prevented the cortisol stress response evident in the
control fish. Anesthetic (with or without buffer) administered before initial
capture was most effective at increasing survival during a second stressor,
while anesthetic supplied after initial capture may have been slightly less
effective. A 0.5% NaCl solution supplied after capture was less effective than
any anesthetic treatment in increasing future survival, but was better than no
treatment. Saline treatment did not attenuate the cortisol stress response. A
rapid method of plasma sample preparation for competitive protein binding assay
of cortisol was developed.
Descriptors: anaesthetics, fish handling, fatigue
(biological), Oncorhynchus tshawytscha,
survival, blood, bioassays, fishery management, fish culture
ASFA; Copyright © 2003, FAO
Suzuki A, Sekizawa Y (1979) Residue
analyses on 2-amino-4-phenylthiazole, a piscine anesthetic, in fishes. 4. GC/MS
analysis on rainbow trout. Bulletin of the Japanese Society of
Scientific Fisheries. 45(2):167-171
NAL Call No. 414.9 J274
When a piscine anesthetic, 2-amino-4-phenylthiazole, was absorbed by rainbow
trout, Salmo gairdneri irideus, from
an anesthetic solution, a whole body concentration of 6.7 and 13 ppm was
attained at 3 hours and 6 hours, respectively. After anesthetization for 6
hours, fish were transferred into 500 l of fresh water flowing at a rate 2 L
per minute. Residues of the anesthetic in the fish were measured by gas
chromatography-mass spectrometry (GC/MS). The total concentration of anesthetic
in the fish declined at a rate comparable to that predicted by a computed theoretical
biexponential curve obtained by a previously described mathematical procedure.
The biological half-life of the anesthetic under these experimental conditions
was approximately 40 minutes in the primary step and 22 hours in the secondary
step. The whole body residues which remained at 24 and 48 hours were 0.1 ppm
and 0.05 ppm, respectively.
Descriptors: anesthetics, excretion, body burden,
biological half life, Salmo gairdneri
irideus, 2-amino-4-phenylthiazole
ASFA; Copyright © 2003, FAO
Suzuki A, Shimura M, Kikuchi T,
Sekizawa Y (1977) Residue analyses on 2-amino-4-phenylthiazole, a piscine
anesthetic, in fishes. 3. Metabolism in rainbow trout and carp. Bulletin
of the Japanese Society of Scientific Fisheries. 43(7):837-847
NAL Call No. 414.9 J274
The major biotransformation product of
2-amino-4-phenylthiazole in rainbow trout (Salmo
gairdneri irideus) was isolated from water following exposure of fish to
the anesthetic. The isolated crystalline metabolite was shown by means of
ultraviolet, infrared and optical rotatory dispersion spectroscopy and gas
chromatography to be identical to 2-amino-4-phenylthiazole-2-N- beta
-mono-D-glucopyranosiduronic acid, the major biotransformation product
previously found in medaka (killifish, Oryzias
latipes). The major biotransformation product in carp (Cyprinus carpio) was also identified as
2-amino-4-phenylthiazole-2-N- beta -mono-D-glucopyranosiduronic acid by
molecular sieve, thin layer and gas chromatography. Conversion of
2-amino-4-phenylthiazole to the N-glucuronyl conjugate was 8 and 12%,
respectively, in rainbow trout and carp as shown by thin layer chromatography
of extracts from fish treated with 3H-labeled anesthetic. In
addition, a minor metabolite of the anesthetic in rainbow trout was isolated as
a yellowish-white crystalline powder and identified as
2-acetamido-4-(4'-hydroxyphenyl)-thiazole by means of ultraviolet and infrared
spectroscopy, NMR and mass spectrometry. Chromatography suggested that this
same metabolite was also formed in carp but in concentrations too low for
isolation and definitive identification.
Descriptors: anaesthetics, metabolism, Salmo gairdneri, Cyprinus carpio
ASFA; Copyright © 2003, FAO
Svobodova Z, Kalab P, Dusek L,
Vykusova B, Kolarova J, Janouskova D (1999) The effect of handling and
transport on the concentration of glucose and cortisol in blood plasma of
common carp. Acta Veterinaria (Brno). 68:265-274
NAL Call No. SF604 B7
The aim of this contribution was to assess the degree
of stress in common carp (Cyprinus carpio,
L.) exposed to handling and transport. Cortisol and glucose concentrations in
blood plasma were used as stress indicators. In some cases, concentration of
ammonia in blood plasma and relative weight of spleen (SSI) were used as well.
Within handling, an effect of time pause (0; 2 and 5 min) between catching the
fish from water and blood sampling for stress indicators was checked. Another
goal was to assess the effect of Menocain anaesthetic on the stress indicators.
An open system of a 10-hour transport in a special long-distance live fish
transport truck (Transport I a II), and a 2-hour transport in classic
transporting tanks (Transport III) were compared. Temperature and oxygen
concentration in water was measured during transport. After handling the fish
prior to blood sampling (2 and 5 min pause), the cortisol concentration dropped
significantly (p< 0.001) and the glucose concentration significantly (p<
0.001) increased compared to values in fish sampled immediately after catching.
No effect of anaesthetics on cortisol concentration was proved. On the
contrary, the glucose concentration fell (p< 0.012) in the anaesthetized
fish. After a 10-hour transport in a special truck (Transport I), a significant
(p< 0.001) decrease of the cortisol concentration was found, as well as a
significant (p< 0.001) increase in the glucose concentration in blood
plasma. In the course of 10-hour transport in a special truck (Transport II),
the majority of carp was found dead. Suffocation and ammonia autointoxication
due to loading the fish with full digestive tract were the most probable causes
of the fish mortality. After a 2-hour transport in transporting tanks
(Transport III) both the cortisol concentration and relative weight of spleen
(SSI) dropped non-significantly while glucose concentration in blood plasma
significantly (p< 0.001) increased. Results showed that both handling and
transport are important stressors in the common carp. Preventive measures were
proposed aimed at alleviating the negative effects of these stressors.
Descriptors: biological stress, anaesthetics, fish
culture, transportation, glucose, blood, ammonia, spleen, Cyprinus carpio, cortisol, common carp, European carp, handling
stress
ASFA; Copyright © 2003, FAO
Svobodova Z, Valentova V, Kouril
J, Vykusova B, Hamackova J (1988) The comparison of the effect of three
anaesthetics on some hematological indicators and acidobasic balance in tench.
Bulletin of the Fisheries Research Institute, Vodnany. 24(1):10-17
NAL Call No. SH1 B77
An evaluation of the new Czechoslovak anaesthetic
Menocain and a study of its effect on some hematological indicators and
acidobasic balance in fish was carried out. The model fish in this comparative
trial were female and male brood tench, Tinca tinca, at artificial spawning. In
the fish anaesthetized by Menocain, no significant changes in the observed
hematological indicators were recorded. On the basis of the carried out
investigation with foreign preparations (Propoxate, Propanidide) it can be
concluded that the new Czechoslovak anaesthetic Menocain is a very suitable
preparation for anaesthesia of carp fish from the aspect of safety.
Descriptors: anaesthesia, anaesthetics, aquaculture
techniques, Tinca tinca, fish
physiology, fish culture, Czechoslovakia, haematology, menocain, propoxate,
propanidide
ASFA; Copyright © 2003, FAO
Svobodova Z, Valentova V, Vykusova
B, Pecena M (1986) Ichtyotoxicological evaluation of the new Czechoslovak
anaesthetic for fish. Bulletin of the Fisheries Research Institute, Vodnany.
23(1):3-7
NAL Call No. SH1 B77
The Czechoslovak anaesthetic for fish introduced under
the title of Monetan has been evaluated. The tests were carried out in carp (Cyprinus carpio). The methodology was
based on the data recommended by the authors of the new Czechoslovak
anaesthetic to achieve anaesthesia in carp, that is the dose of 0.1 g per 1 L
of water at the temperature of 18-24°C in time period of 10 min. LC50
value of Monetan for carp fry (K1) in time period of 10 min and
water temperature of 22°C was 0.81 g/L, minimum lethal concentration LC5
in time period of 10 min and water temperature of 22°C was 0.49 g/L. T50
value of Monetan (i. e. the time in the course of which 50% die using the
recommended dose of 0.1 g/L and water temperature of 22°C) was 101 min, t5
value (i. e. the time in the course of which 5% die using the recommended dose
of 0.1 g/L and water temperature of 22°C) was 83 min.
Descriptors: anesthesia, hematology, lethal limits, Cyprinus carpio, anesthetics, toxicity
tolerance, test organisms, Czechoslovakia, Monetan
ASFA; Copyright © 2003, FAO
Svobodova Z, Vykusova B, Kouril J
(1992) Menocain -- Czechoslovak anaesthetics for fish. Proceedings
of the Scientific Conference on Fish Reproduction '92 (Vodnany, Czechoslovakia,
2-4 March, 1992)., Res. Inst. of Fish Culture and Hydrobiol., Vodnany (Czech
Rep.). pp. 152-153
Menocain is a specific fish anaesthetics which does not provoke anaesthesia in
homoiothermic animals. There was confirmed on the base of toxicological,
hematological, and histopathological investigations that this anaesthetics is
safe for fish. Its suitability for fish anaesthesia was proved by several years
of its successful application in fishery practice.
Descriptors: fish culture, aquaculture techniques, anaesthetics,
haematology, histopathology, toxicology, Menocain
ASFA; Copyright © 2003, FAO
Sylvester JR, Holland LE (1982) Influence
of temperature, water hardness and stocking density on MS-222 response in
three species of fish. Progressive Fish Culturist. 44(3):138-141
NAL Call No. 157.5 P94
Three responses of rainbow trout (Salmo
gairdneri), common carp (Cyprinus
carpio), and fathead minnows (Pimephales
promelas) to the anesthetic MS-222 (tricaine methanesulfonate) were
identified: induction times to total loss of equilibrium decreased with
increasing temperatures; resistance to MS-222 increased with increasing water
hardness; and resistance to the drug increased when the densitv of test fish
was increased from 0.3 to 0.9 g/L in rainbow trout, 0.4 to 1.2 g/L in carp, and
0.3 to 0.8 g/L in fathead minnows. These results, and others from the
literature, suggest that temperature, water hardness, and density of test fish,
as well as pH and dissolved oxygen, may have significant interacting effects on
the anesthetic properties of MS-222.
Descriptors: temperature effects, water hardness, stocking
density, anesthetics, Salmo gairdneri,
Cyprinus carpio, Pimephales promelas, response analysis, environmental
effects
ASFA; Copyright © 2003, FAO
Takashima Y, Wan Z, Kasai H,
Asakawa O (1983) Sustained anesthesia with 2-phenoxyethanol in yearling
rainbow trout. Journal. Tokyo University of Fisheries.. Tokyo Suisandai
Kempo. 69(2):93-96
ISSN: 0040-9014
The possibility of using 2-phenoxyethanol for long-term anesthesia in yearling
rainbow trout, Salmo gairdneri, was
examined. The 24 hour TLm was 320 ppm for trout yearling (100-200 g in body
weight, at 12°C) and none of the fish immersed below 200 ppm died until 24
hours. All of the fish anesthetized below 200 ppm showed normal appearance and
behavior after transferring to clean running water. Therefore, it was thought
to be possible to anesthetize with 2-phenoxyethanol for at least 24 hours. On
the other hand, serum cortisol levels analyzed by radioimmunoassay increased rapidly
after immersion into 150 ppm of 2-phenoxyethanol for 1 hour. Moreover,
prominent elevation of serum cortisol level was also recognized following
mechanical agitation of rearing water in spite of anesthetic condition. It may
be concluded that anesthesia with 2-phenoxyethanol does not reduce the stress
during transport.
Descriptors: fish physiology, anesthesia, stress, fish
handling, biological stress, Salmo
gairdneri, phenoxyethanol
ASFA; Copyright © 2003, FAO
Tamaru CS, Carlstrom-Trick C,
FitzGerald WJ Jr (1996) Clove oil, minyak cengkeh, a natural fish
anesthetic. Proceedings of the Pacon Conference on Sustainable
Aquaculture '95.pp. 365-371
NAL Call No. TC1505 P33 1995
Indonesian clove oil, minyak cengkeh, was found to be an effective anesthesia
for use in hatchery practices with the rabbit fish Siganus argenteus. At a concentration of 25 ppm fish could be
physically handled for length and weight determinations and for the performance
of gonadal biopsy. Time to loss of equilibrium was less than three minutes at
the lowest concentration tested and time to recovery was less than five minutes
at all concentrations tested. No adverse effects or mortalities were observed
during the two month study period. Clove oil was also found to be more
effective than 2-phenoxyethanol under the same conditions and at the same
dosages. The use of minyak cengkeh is a perfect example of how we in the
developed countries tend to overlook the available resources in recipient
countries of our technology transfer activities. Technology transfer, or
technical assistance, most often involves the transfer of technology developed
in a developed country to an undeveloped or a developing country by so-called
experts in the developed country that developed the technology. Because the
technology transferred is too often unfamiliar to the recipient, the materials
needed to sustain the technology too often unavailable, and chronically the
resources in the developing country are scrapped for the modern and more
sophisticated ways of the “Western World”, we may have taught someone how to
cook a gourmet dinner but he still won't be able to feed the family.
Descriptors: fish culture, hatcheries, aquaculture
techniques, anaesthetics, Siganus
argenteus
ASFA; Copyright © 2003, FAO
Taylor PB (1988) Effects of
anaesthetic MS 222 on the orientation of juvenile salmon, Oncorhynchus tshawytscha Walbaum. Journal of Fish Biology. 32(2):161-168
NAL Call No. QL614 J68
Juvenile salmon, Oncorhynchus tshawytscha,
were trained to orientate in a direction (270 degree) and then anaesthetized
with ethyl m-aminobenzoate methane sulphonate (MS 222) in a test to determine
whether anaesthesia affected the learnt orientation. Before anaesthesia the
control group of twelve fish showed a mean unimodal orientation of 264°and a
bimodal axis of orientation of 258 °/78° with a confidence limit (second order
analysis) of between 218 degree and 285°. After administration of MS 222 nine
out of ten fish showed marked changes in orientation and random behaviour,
persisting in two fish for more than 14 days. Eight weeks after completion of
the anaesthesia trial the fish were retested. Each fish, except one, showed a
mean bimodal axis of orientation that fell within the confidence limit of the
control. The results support the view that the reference orientation in this
study (270°) was a learnt, not an innate directional preference. The effects
anaesthesia may have on salmon behaviour during migration are discussed.
Descriptors: anaesthetics, orientation behaviour, learning
behaviour, Oncorhynchus tshawytscha,
migrations
ASFA; Copyright © 2003, FAO
Tsuda Heizo (1987) Experiments
on the method of anesthetization of juvenile bluefin tuna. Bulletin of
the Fisheries Research Institute, Mie, Japan. 2:9-18
The juvenile bluefin tuna are weak in the skin, which is an obstacle to the
research procedure such as weighing, measuring, transferring and so on. To
safeguard the tuna against abrasion, the useful anesthesia has been studied
over the period of 1983 to 1985. The tuna cultured in the tank, 210 g to 1970 g
by weight, were used. Ulethane, MS-222 (m-aminobenzoic acid ethylester
metanesulfonate), and eugenol (4-allyl-2-methoxyphenol) were examined as to the
anesthetic effects to the tuna. In addition to this, when using cooled sea water
and cooled sea water added ulethane, the effect was examined. The tuna were
caught one by one with rod from the tank and transferred to the anesthetic
solution prepared. After anesthetized, the tuna were weighed and moved back to
the sea water, and then recovered from anesthesia. <<Abstract is partly
entered herein due to the whole abstract is too long.>>
Descriptors: tuna fisheries, fish larvae, fish culture,
anaesthesia, anaesthetics, Thunnus
thynnus, bluefin tuna, urethane, MS-222, Eugenol
ASFA; Copyright © 2003, FAO
Tytler P, Hawkins AD (1981) Vivisection,
anaesthetics and minor surgery. Aquarium Systems, pp. 247-278.
NAL Call No. SF457 A67
The
following topics are considered: vivisection, immobilization and anaesthesia,
humane killing of fish, surgery and surgical equipment, marking fish,
electrocardiography, blood sampling and physiological saline solution.
Descriptors: fish handling, experimental research,
anesthesia
ASFA; Copyright © 2003, FAO
Umbreit N (1980) Chemical
Restraint of Reptiles, Amphibians, Fish, Birds, Small Mammals and Selected
Marine Mammals in North America: An Annotated Bibliography (Technical note). Bureau of Land Management, Denver, CO. Report Number: TN-340,181 p. Note: Order this product from NTIS by: phone at 1-800-553-NTIS (
This annotated bibliography provides a list of available literature sources on
anesthetizing or immobilizing small animals. Although the original intent of
this bibliography was to deal strictly with small wild animals, it was expanded
to include listing on many laboratory animals such as rabbits, rats, and mice.
Extensive testing and research on these animals has led to better methods in
handling, anesthetizing and immobilizing. For these reasons, it is hoped that
the procedures and methods obtained in the laboratory may apply to the welfare,
well-being and management of wild animal populations. Information has also been
included on repellants, as they too are a form of chemical restraint.
Descriptors: mammals, wildlife, laboratory animals, constraining,
bibliographies, amphibia, fish, birds, reptiles, tranquilizer drugs, anesthetics,
repellants, dosage
Veenstra RS, Balon EK,
Flegler-Balon C (1987) Propanidid, a useful anaesthetic for studying blood
circulation in early development of fish. Canadian Journal of
Zoology. 65(5):1286-1289
NAL Call No.
470 C16D
The effectiveness of propanidid was tested by comparing it with cocaine
hydrochloride, urethane, and traicaine methanesulfonate, anaesthetics already
established for studies of early ontogeny in fishes. Free embryos of the brook
charr, Salvelinus fontinalis, and
7-day-old amargosa pupfish, Cyprinodon
nevadensis amargosae, were anaesthetized with dilute solutions of these
drugs. The times taken until the blood elements ceased moving through the
capillary loops of the developing caudal fin were compared. Propanidid was
found to be superior to the other drugs tested in maintaining the longest
duration of unaltered blood flow.
Descriptors: anaesthetics, methodology, comparative
studies, Salvelinus fontinalis, Cyprinodon nevadensis amargosae, blood
circulation, propanidid
ASFA; Copyright © 2003, FAO
Wagner E, Arndt R, Hilton B (2002)
Physiological stress responses, egg survival and sperm motility for rainbow
trout broodstock anesthetized with clove oil, tricaine methanesulfonate or
carbon dioxide. Aquaculture. 211(1-4):353-366
NAL Call No. SH1A6
Egg survival, sperm motility and physiological stress responses (plasma
cortisol, glucose and chloride) of rainbow trout (Oncorhynchus mykiss) broodstock were compared among three
anesthetics: tricaine methanesulfonate (MS-222), clove oil in the form of AQUI-S
(a proprietary mix of 50% isoeugenol and other ingredients) and carbon dioxide
gas. Concentrations of 60 mg/l tricaine, 20 mg/l isoeugenol (40 mg/l AQUI-S)
and 220-275 mg/l carbon dioxide were based on preliminary tests and chosen to
standardize induction time among anesthetics. Plasma glucose, chloride and
cortisol concentrations indicated that none of the anesthetics used after
crowding and netting completely eliminated the stress response. The return to
prestress cortisol levels differed among the three anesthetics. Fish
anesthetized with AQUI-S had significantly lower cortisol concentrations at 1
or 7 h postimmersion than the other anesthetics and controls, but were elevated
at 24 h. Plasma cortisol in tricaine- and CO2-treated fish returned
to prestress levels within 7 and 24 h, respectively, whereas cortisol levels in
control fish remained elevated at 24 h. Sperm motility and duration of motility
were assessed for a practical range of concentrations: tricaine, 15-100 mg/l;
AQUI-S, 10-100 mg/l; CO2, 50-173 mg/l. The percentage of motile
sperm was unaffected by anesthetic treatment, averages ranging from 68% to 87%.
However, duration of motility decreased as anesthetic concentration increased,
averages ranging from 55 to 36 s for tricaine and from 56 to 37 s for AQUI-S.
Duration of sperm motility was low (31-43 s) for all levels of CO2
tested. Fish recovery time was significantly longer for fish anesthetized by
AQUI-S (370 s) than the either CO2 or tricaine (192 and 199 s,
respectively). Gender had no effect on recovery time. Egg survival to the eyed
stage and to hatch was not significantly different among anesthetic treatments
and controls. No delayed mortality was observed for any of the fish handled and
bled for the test. Results indicated that tricaine, AQUI-S and CO2 were all
suitable for broodfish anesthesia, but the longer recovery time and lower cost
for AQUI-S may make it more useful than the alternatives. None of the
anesthetics wholly suppressed the stress responses during a typical spawning process,
but did help reduce the duration of the stress responses and eased handling
without compromising egg viability.
Descriptors: brood stocks, fish eggs, sperm, aquaculture
techniques, induced breeding, anaesthesia, handling, carbon dioxide, biological
stress, locomotion, survival, biological fertilization, Oncorhynchus mykiss, cortisol, clove oil, rainbow trout, tricaine
methanesulfonate
ASFA; Copyright © 2003, FAO
Walsh CT, Pease BC (2002) The
use of clove oil as an anaesthetic for the longfinned eel, Anguilla reinhardtii (Steindachner). Aquaculture Research. 33(8):627-635
NAL Call No. SH1 F8
To handle large river eels during
procedures such as measuring and tagging for field and aquaculture studies,
they must be anaesthetized. During our initial biological studies of Anguilla reinhardtii (Steindachner) it
was found that the anaesthetic benzocaine was relatively expensive and elicited
a variable response, even when used at relatively high concentrations. Human
health risks are also a concern when using benzocaine, as some of the eels may
later be sold for human consumption. Therefore, experiments were done to
evaluate the use of clove oil (a safe, naturally occurring product) for
anaesthesia of this species at a range of temperatures (17 and 25°C) and
salinities (0-32 g/L. It was found that clove oil provided a suitable
anaesthetic response through this wide range of temperatures and salinities.
Response times were found to be relatively variable for both benzocaine and
clove oil. This variability may be related to stress, environmental factors, or
the condition of the fish. Clove oil is recommended for anaesthesia of
anguillid eels because it is effective, relatively inexpensive, and poses
little risk to human health.
Descriptors: costs, aquaculture techniques, anaesthetics,
Anguillidae, Anguilla reinhardtii,
Syzygium aromaticum, clove oil, freshwater eels
ASFA; Copyright © 2003, FAO
Waterstrat PR (1999) Induction
and Recovery from Anesthesia in Channel Catfish Ictalurus punctatus Fingerlings Exposed to Clove Oil. Journal
of the World Aquaculture Society. 30(2):250-255
NAL Call No. SH138 W62
The use of clove oil as an anesthetic for
channel catfish Ictalurus punctatus
fingerlings was examined. At 100 mg/L, clove oil induced anesthesia within 1
min following exposure. Fish recovered from a 10-min period of anesthesia in
100 mg/L clove oil within 4 min following removal from the anesthetic solution.
At clove oil concentrations of 150 mg/L or greater, recovery times were prolonged,
requiring longer than 10 min for recovery. At 300 mg/L, mortality was observed
with half of the catfish fingerlings failing to recover from the 10-min
exposure. Fish could be safely maintained in 100 mg/L clove oil for periods of
up to 15 min; exposure for longer than 15 min produced both prolonged recovery
times and mortality. At a concentration of 100 mg/L clove oil produced
responses similar to those of the commonly used fish anesthetic MS-222. The low
cost of clove oil relative to MS-222 and the extensive testing and use of clove
oil in dentistry and as a food ingredient make clove oil an attractive
candidate as a fish anesthetic.
Descriptors: fish culture, anaesthesia, aquaculture
techniques, fingerlings, mortality, Ictalurus
punctatus, channel catfish
ASFA; Copyright © 2003, FAO
Weyl O, Kaiser H, Hecht T (1996) 'Breath
holding' in tilapia Oreochromis
mossambicus. South African Journal of Science. 92(3):152-154
NAL Call No. 515 SO84
During experiments on the use of anaesthetics in fishes, it was observed that Oreochromis mossambicus was able to
cease opercular movement for up to 15 minutes. It could be shown that this was
not an immediate anaesthetic effect. The cessation of ventilation corresponded
to a reduction of oxygen consumption. The pathway of detection of foreign
substances appears to be olfactory. We discuss possible reasons for this
specific, previously unobserved behaviour, as well as its consequences for
culturing this and other fish species.
Descriptors: anesthetics, fish culture, oxygen
consumption, olfaction, gills, respiration, Oreochromis
mossambicus
ASFA; Copyright © 2003, FAO
Weyl O, Kaiser H, Hecht T (1996) On
the efficacy and mode of action of 2-phenoxyethanol as an anaesthetic for
goldfish, Carassius auratus (L.), at
different temperatures and concentrations. Aquaculture Research. 27(10):757-764
NAL Call No. SH1 F8
The objective of this study was to assess
the efficacy and mode of action of 2-phenoxyethanol as an anaesthetic for two
size classes of goldfish, Carassius
auratus (L.), at three different temperatures. Goldfish (2.15 " 0.05 g, and 9.19 " 0.17 g) were exposed to 0.3, 0.4 and 0.5 ml
2-phenoxyethanol/L at 20, 25, and 30°C. Time needed to induce anaesthesia was
dependent on concentration and water temperature. At temperatures at and below
25°C, 0.4 ml/L was needed to induce total loss of equilibrium within less than
15 min. Above 25°C, 0.5 ml/L was required to induce anaesthesia. Recovery rate
was independent of the length of anaesthesia, which indicates that the
anaesthetic is taken up and lost via a concentration gradient at the gill
membrane and skin/solution interface. Fish recovered within less than 10 min
after they had been taken out of the anaesthetic solution. In a second
experiment, goldfish responded to a repeated exposure to 2-phenoxyethanol daily
over a period of 14 days with increased tolerance, which indicates a
habituation response to the anaesthetic. The use of 2-phenoxyethanol as an anaesthetic
both for short-term anaesthesia and for anaesthesia under transport conditions
is discussed.
Descriptors: anaesthetics, bioassays, fish culture, Carassius auratus, 2-phenoxyethanol
ASFA; Copyright © 2003, FAO
Yamamitsu S, Itazawa Y (1988) Effects
of an anesthetic 2-phenoxyethanol on the heart rate, ECG and respiration in
carp.
NAL Call No. 414.9 J274
Heart rate, ECG and respiratory
parameters were measured with carp (Cyprinus
carpio) of about 600 g during various stages of anesthesia induced with
400-800 ppm solution of 2-Phenoxyethanol. In 400 ppm solution, deep sedation,
tachycardia, shortening of QT sub(1), increase in
ventilation frequency and slight decrease in oxygen consumption were observed.
In 600 ppm solution, disappearance of tachycardia, extension of time elements
of ECG, and decrease in all respiratory parameters were observed. In anesthesia
induced with 400-600 ppm solution, fish recovered from anesthesia by irrigation
with fresh water containing no anesthetic. In 800 ppm solution, progressive
bradycardia, remarkable extension of time elements of ECG and decrease in
voltage elements of ECG, and drops to almost zero in respiratory parameters
owing to cessation of ventilation movement were observed and fish did not
recover from anesthesia by irrigation with fresh water.
Descriptors: fish physiology, bioassays, Cyprinus carpio, anaesthetics,
2-phenoxyethanol
ASFA; Copyright © 2003, FAO
Yokoyama Y, Yoshikawa H, Ueno S,
Mitsuda H (1989) Application of CO2-anesthesia combined with low
temperature for long-term anesthesia in carp. Nippon Suisan
Gakkaishi/Bulletin of the Japanese Society of Scientific Fisheries.
55(7):1203-1209
NAL Call No. 414.9 J274
The efficacy of CO2 anesthesia combined with low temperature
in long-term anesthesia supposing the transportation of live fish was evaluated
using adult carp Cyprinus carpio
acclimated at 23°C. All the carp became anesthetized in about 10 min with a
30-min cold treatment at 4°C and were safely maintained in an anesthetic state
for the following 9.5 h with the cold-CO2 treatment. The
optimum anesthetic condition for adult carp was pCO2 at 80
mmHg at 14°C.
Descriptors: carbon dioxide, water temperature,
transportation, live storage, Cyprinus
carpio, anaesthesia
ASFA; Copyright © 2003, FAO
Yoshikawa H, Ueno S, Mitsuda H
(1989) Short- and long-term cold-anesthesia in carp. Nippon Suisan
Gakkaishi/ Bulletin of the Japanese Society of Scientific Fisheries.
55(3):491-498
NAL Call No. 414.9 J274
The efficacy of cold anesthesia in the transportation of live fish was
evaluated using carp (Cyprinus carpio)
acclimated at 23°C. The carp could be safely maintained in an anesthetic state
for 5 h in water kept at 4°C and in the anesthetized or sedated state for 24 h
at 8-14°C. Some anesthetized carp showed signs of convulsion when they received
external stimuli, and bled mainly from gills. Hemorrhage became distinct with
the decrease in temperature and the duration of the cold treatment. The sedated
carp showed no such excitement and bleeding. The sedated state is considered to
be adequate for transportation, regardless of the anesthetic time; 14°C seems
to be the optimal temperature when the carp are acclimated to 23°C.
Descriptors: temperature effects, Cyprinus carpio, transportation, anaesthesia, cold-anesthesia
ASFA; Copyright © 2003, FAO
Zinkovsky OG, Potrokhov AS,
Evtushenko NYu (1995) The use of tranquillizers and antidepressants in
experimental studies of fish. Gidrobiologicheskij Zhurnal (Kiev)
Hydrobiology Journal. 31(3):85-94
NAL Call No.
QH90 A1G5
A series of drugs (phenazepamum, hydazepamum, phenobarbital, aminazinum) were
tested on common carp (Cyprinus carpio)
and silver carp (Hypophthalmichthys
molitrix) for the applicability in fish-breeding as anaesthetic agents and
antidepressants. Tentative pharmacological assessment is given. Methods and
techniques allowing employment of the drugs in fish-breeding and related activities
are discussed.
Descriptors: biological stress, fish physiology,
anaesthetics, Cyprinus carpio, Hypophthalmichthys molitrix, Ukraine
ASFA; Copyright © 2003, FAO
2000 Report of the AVMA Panel on Euthanasia
http://www.avma.org/resources/euthanasia.pdf
Canadian Council on Animal Care (CCAC)
http://www.ccac.ca
The
Canadian Council on Animal Care (CCAC) has posted its second draft of CCAC
guidelines
on: the care and use of fish in research, testing, and teaching. The guidelines are available at the CCAC
webpage (http://www.ccac.ca/en/CCAC_Programs/Guidelines_Policies/GDLINES/Guidelis.htm).
CVM Guide 1240.4200
Low Regulatory Priority Aquaculture Drugs
http://www.fda.gov/cvm/Documents/LRPDrugs.pdf
CVM Guide 1240.4260 Drugs Approved for Use in Aquaculture FDA-CVM Guidance Document 150 FishDoc – The
Home of Fish Health Fish Anaesthetics: Food Animal Residue Avoidance Data Bank
(FARAD) MS222 (Tricaine Methane Sulphonate) Ross, L.G. & Ross, B.
1999. Anaesthetic and sedative techniques
for aquatic animals. Second edition. Fishing News Books. Blackwell Ross, L.G. & Ross, B.
1984. Anaesthetic and sedative techniques
for fish. Species Specific Anaesthesia White Paper Summary of Research Related
to Transportation of Juvenile Anadromous Salmonids Around Snake and A letter (with several pieces of follow-up
correspondence) from FDA to USFWS regarding the use of non-approved drugs
on threatened and endangered species is freely available, but you must request these from Dr. Dave Erdahl (406-587-9265 x125,
dave_erdahl@fws.gov)
of the USFWS who has the current INAD for the use of isoeugenol.
Classification of Aquaculture Species as Food or Nonfood
http://www.fda.gov/cvm/Policy_Procedures/4260.pdf
http://www.fda.gov/cvm/4401.htm
Guidance for Industry, Status of Clove
Oil and Eugenol for Anesthesia of Fish
http://www.fda.gov/cvm/Documents/guide150.pdf
http://www.fishdoc.net
http://www.fishdoc.net/articles/details.php?articleId=15
www.farad.org
http://www.alpharmaanimalhealth.co.uk/VPDF/MS%20222.pdf
...
transport anaesthesia vaccination,
minor surgery deep anaesthesia
major surgery,
euthanasia For further ...
Anaesthesia and Restraint.
In: Textbook of fish medicine
...
http://www.aquaculture.stir.ac.uk/GISAP/Pubs/A&S.htm
http://www.aquaculture.stir.ac.uk/GISAP/Pubs/A&S.htm
Woods Hole Oceanographic Institute
Bibliographic listing of resources
http://library.mbl.edu/reference/acubooks.html#seven
http://www.efw.bpa.gov/publications/NMFS.199910.transport.white.paper.pdf