Cell type-specific interactions of transcription factors with a housekeeping promoter in vivo.

Nucleic Acids Res. 1993 May 25; 21(10): 2465–2471.

PMCID: PMC309548

G Stapleton, M P Somma, and P Lavia

AFRC Centre for Genome Research, University of Edinburgh, UK.

This article has been cited by other articles in PMC.

Abstract

Mammalian housekeeping promoters represent a class of regulatory elements different from those of tissues-specific genes, lacking a TATA box and associated with CG-rich DNA. We have compared the organization of the housekeeping Htf9 promoter in different cell types by genomic footprinting. The sites of in vivo occupancy clearly reflected local combinations of tissue-specific and ubiquitous binding factors. The flexibility of the Htf9 promoter in acting as the target of cell-specific combinations of factors may ensure ubiquitous expression of the Htf9-associated genes.

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Selected References

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