RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19980130gel01
Unique Web Code: rsg04Srr
Tissue Aliquot Code: lutjcamp D97-004 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	78	3.5	0.216514	40	14.482	16.1	13.2	3.5	79.1	40	0.204224
2	4.55	350	15.8	0.466258	12	9.26939	10.3	8.4	4.55	352.5	11.9	0.439253
3	5.2	532	24	0.516256	10	8.9361	9.9	8.1	5.2	531.5	10.2	0.493678
4	5.85	858	38.7	0.625123	9	10.6534	11.8	9.7	5.85	857.4	9.2	0.656325
5	6.55	1169	52.8	0.260339	15	6.3642	7.1	5.8	6.55	1164.6	14.7	0.244939
6	6.85	1257	56.7	0.350161	9	5.34993	5.9	4.9	6.85	1257.8	9.4	0.320514
7	7.35	1443	65.1	0.564653	10	9.63112	10.7	8.8	7.35	1440.1	10.2	0.532144
8	8.15	1755	79.2	0.152214	17	4.46988	5	4.1	8.15	1754	16.5	0.152507
9	8.45	1865	84.2	0.234163	13	5.14711	5.7	4.7	8.45	1864.9	13.1	0.221678
10	8.65	1940	87.5	0.162461	11	3.09071	3.4	2.8	8.65	1939.4	10.8	0.160909
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2186	98.6	0.555893	14	12.5385	13.9	11.4	9.3	2182.4	13.7	0.518138

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	84	3.8	0.19537	37	12.1119	6.8	5.9	3.5	88.2	36.8	0.185724
2	3.692	135	6.1	0.280982	9	3.16987	1.8	1.5	3.692	135	8.8	0.204072
3	4.234	279	12.6	0.608048	8	9.36457	5.3	4.5	4.234	278.8	8.5	0.62224
4	4.55	363	16.4	0.377218	10	5.93761	3.4	2.9	4.55	363.7	9.7	0.345367
5	4.948	472	21.3	0.186597	11	3.72283	2.1	1.8	4.948	470.5	11.4	0.18504
6	5.2	541	24.4	0.441668	10	7.49108	4.2	3.6	5.2	540.4	10.1	0.417975
7	5.247	564	25.5	0.14589	11	2.72051	1.5	1.3	5.247	564.6	11	0.139114
8	5.326	603	27.2	0.366653	9	5.69727	3.2	2.8	5.326	602.4	9.3	0.345702
9	5.64	757	34.2	0.639422	10	12.1247	6.8	5.9	5.64	758.2	10.5	0.652872
10	5.85	860	38.8	0.433625	9	7.63771	4.3	3.7	5.85	858.8	9.5	0.455486
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.975	915	41.3	0.82284	12	17.1413	9.7	8.3	5.975	916.9	11.9	0.811727
12	6.077	960	43.4	0.476443	12	9.69212	5.5	4.7	6.077	958.2	12.3	0.445825
13	6.152	993	44.9	0.159482	12	3.56263	2	1.7	6.152	991.6	12	0.167036
14	6.478	1137	51.4	0.124914	18	4.00386	2.3	1.9	6.478	1138.2	18.2	0.124049
15	6.546	1167	52.7	0.225723	7	2.57851	1.5	1.2	6.546	1166.2	6.9	0.209874
16	6.636	1193	53.9	0.120881	6	1.09769	0.6	0.5	6.636	1192.2	5.9	0.105206
17	6.72	1217	55	0.538802	6	3.11887	1.8	1.5	6.72	1216.4	5.8	0.304624
18	6.85	1254	56.6	0.220109	21	15.6017	8.8	7.5	6.85	1235.6	20.9	0.420953
19	6.913	1277	57.7	0.0511486	1	0	0	0	6.913	1266	1	0
20	7.328	1429	64.5	0.320698	12	6.92104	3.9	3.3	7.328	1427.9	12.5	0.313339
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	1437	64.9	0.38577	7	2.97185	1.7	1.4	7.35	1440.4	6.7	0.24947
22	7.885	1641	74.1	0.301851	7	8.12173	4.6	3.9	7.885	1646.1	7.4	0.620013
23	8.15	1742	78.7	0.114033	35	7.17318	4.1	3.5	8.15	1728.5	35.3	0.114526
24	8.45	1866	84.3	0.318533	21	11.2736	6.4	5.5	8.45	1865.6	20.7	0.307741
25	8.65	1940	87.6	0.150975	11	2.97943	1.7	1.4	8.65	1939.8	11.2	0.150148
26	9.3	2191	99	0.62436	11	10.8626	6.1	5.3	9.3	2188.9	11.1	0.55169

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.683	130	5.9	0.263066	10	4.83264	5.6	3.8	3.683	130	10.4	0.263046
2	4.225	272	12.3	0.650998	8	9.72221	11.3	7.6	4.225	272.3	8.3	0.66162
3	4.939	464	21	0.146757	10	2.54217	3	2	4.939	462.4	10.2	0.140714
4	5.237	554	25.1	0.109963	9	1.65816	1.9	1.3	5.237	552.8	8.7	0.10726
5	5.318	594	26.9	0.441393	8	5.7556	6.7	4.5	5.318	593.6	7.7	0.421509
6	5.64	752	34	0.781137	9	12.8977	15	10.1	5.64	754.1	9.1	0.803237
7	5.844	852	38.5	0.0767024	14	1.87034	2.2	1.5	5.844	854.5	14	0.0753186
8	5.977	912	41.2	0.932667	9	14.8261	17.2	11.7	5.977	913.8	9.1	0.918187
9	6.073	955	43.2	0.599251	9	9.12405	10.6	7.2	6.073	953.7	9.4	0.550492
10	6.149	989	44.7	0.182398	12	3.96226	4.6	3.1	6.149	986.1	12.1	0.185258
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.527	1158	52.4	0.31176	5	2.872	3.3	2.3	6.527	1158	5.3	0.306442
12	6.599	1182	53.5	0.147972	5	1.17895	1.4	0.9	6.599	1181.4	5	0.133846
13	6.678	1204	54.5	0.642921	9	8.69446	10.1	6.8	6.678	1207	8.9	0.553097
14	6.888	1266	57.3	0.0158926	5	0.167242	0.2	0.1	6.888	1263.1	4.8	0.019675
15	7.281	1410	63.8	0.16234	8	2.1849	2.5	1.7	7.281	1409.8	7.5	0.163988
16	7.833	1620	73.3	0.21344	10	3.87644	4.5	3	7.833	1620.2	10.4	0.210288

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	80	3.6	0.21928	36	13.1792	17.3	13.8	3.5	80.3	36	0.206548
2	4.55	351	15.9	0.508595	11	8.74542	11.5	9.2	4.55	352.8	10.5	0.468147
3	5.2	531	24.1	0.601954	8	8.53033	11.2	8.9	5.2	530.8	8.3	0.577379
4	5.85	850	38.5	0.674926	8	10.1959	13.4	10.7	5.85	849.3	8.2	0.705085
5	6.55	1168	53	0.395152	9	5.76072	7.6	6	6.55	1165.3	8.6	0.376505
6	6.85	1250	56.7	0.327538	9	4.82294	6.3	5.1	6.85	1249.6	9.1	0.29859
7	7.35	1434	65	0.501361	10	8.4627	11.1	8.9	7.35	1431	10.2	0.468691
8	8.15	1740	78.9	0.126751	14	3.14046	4.1	3.3	8.15	1740.8	14.2	0.125202
9	8.45	1852	84	0.210575	11	4.07765	5.4	4.3	8.45	1852	11	0.208999
10	8.65	1924	87.3	0.172962	10	2.93887	3.9	3.1	8.65	1925	10	0.166258
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2174	98.6	0.460838	9	6.33314	8.3	6.6	9.3	2172.2	8.6	0.417846

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.704	130	5.9	0.246419	10	4.32481	4.8	3.5	3.704	129.4	10	0.244906
2	4.259	274	12.4	0.640494	8	9.65684	10.7	7.8	4.259	273.4	8.5	0.643285
3	4.954	462	20.9	0.123977	11	2.25476	2.5	1.8	4.954	462.4	10.5	0.120891
4	5.244	552	25	0.111389	9	1.66873	1.8	1.3	5.244	551.1	8.9	0.105321
5	5.326	592	26.8	0.445426	8	5.96337	6.6	4.8	5.326	592.1	8.1	0.413724
6	5.647	748	33.9	0.767825	9	12.0752	13.3	9.8	5.647	749.1	8.7	0.781924
7	5.84	842	38.2	0.139981	9	2.05291	2.3	1.7	5.84	844	9.2	0.126195
8	5.977	906	41.1	0.935317	9	14.3232	15.8	11.6	5.977	907.2	8.8	0.922643
9	6.072	950	43	0.642158	9	9.17717	10.1	7.4	6.072	949	8.7	0.592086
10	6.149	986	44.7	0.192888	13	4.67656	5.2	3.8	6.149	981.3	12.9	0.204273
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.511	1154	52.3	0.323025	5	2.64144	2.9	2.1	6.511	1153.5	4.6	0.324502
12	6.565	1176	53.3	0.162446	6	1.47734	1.6	1.2	6.565	1175.5	5.9	0.141913
13	6.646	1198	54.3	0.677781	9	9.05182	10	7.3	6.646	1201	9.1	0.56416
14	6.866	1259	57	0.0650405	9	1.03272	1.1	0.8	6.866	1259.4	8.8	0.0661127
15	7.268	1407	63.8	0.167141	8	2.51695	2.8	2	7.268	1407.1	8.5	0.168012
16	7.801	1610	72.9	0.243753	9	3.75884	4.1	3	7.801	1610.5	8.9	0.238981
17	8.455	1856	84.1	0.182264	13	3.95172	4.4	3.2	8.455	1853.2	12.7	0.174994

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	74	3.4	0.251829	36	15.2628	16.7	13.7	3.5	75.7	36	0.239203
2	4.55	348	15.8	0.590702	10	9.81315	10.7	8.8	4.55	349.7	10.4	0.533117
3	5.2	530	24	0.697949	8	9.37821	10.3	8.4	5.2	529	7.8	0.682677
4	5.85	844	38.3	0.740774	8	10.2913	11.3	9.3	5.85	844.3	7.6	0.760665
5	6.55	1176	53.4	0.523146	8	7.07641	7.7	6.4	6.55	1174.1	8.1	0.492952
6	6.85	1256	57	0.361226	10	5.53135	6	5	6.85	1255.9	9.7	0.320939
7	7.35	1440	65.3	0.582263	11	10.5986	11.6	9.5	7.35	1436.6	11	0.543052
8	8.15	1748	79.3	0.189062	14	4.5847	5	4.1	8.15	1746.9	14	0.184679
9	8.45	1856	84.2	0.311189	12	6.14811	6.7	5.5	8.45	1855.3	11.7	0.296919
10	8.65	1930	87.6	0.212274	11	3.91843	4.3	3.5	8.65	1928.9	10.7	0.206296
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2170	98.5	0.582459	9	8.85341	9.7	8	9.3	2167	9	0.556255

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 16.100 4.550 10.300 5.200 9.900 5.850 11.800 6.550 7.100 6.850 5.900 7.350 10.700 8.150 5.000 8.450 5.700 8.650 3.400 9.300 13.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 14 PeakCount: 11 Return to Lane Navigator
3.500 6.800 3.692 1.800 4.234 5.300 4.550 3.400 4.948 2.100 5.200 4.200 5.247 1.500 5.326 3.200 5.640 6.800 5.850 4.300 5.975 9.700 6.077 5.500 6.152 2.000 6.478 2.300 6.546 1.500 6.636 0.600 6.720 1.800 6.850 8.800 6.913 0 7.328 3.900 7.350 1.700 7.885 4.600 8.150 4.100 8.450 6.400 8.650 1.700 9.300 6.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 15 PeakCount: 26 Return to Lane Navigator
3.683 5.600 4.225 11.300 4.939 3.000 5.237 1.900 5.318 6.700 5.640 15.000 5.844 2.200 5.977 17.200 6.073 10.600 6.149 4.600 6.527 3.300 6.599 1.400 6.678 10.100 6.888 0.200 7.281 2.500 7.833 4.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 16 PeakCount: 16 Return to Lane Navigator
3.500 17.300 4.550 11.500 5.200 11.200 5.850 13.400 6.550 7.600 6.850 6.300 7.350 11.100 8.150 4.100 8.450 5.400 8.650 3.900 9.300 8.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator
3.704 4.800 4.259 10.700 4.954 2.500 5.244 1.800 5.326 6.600 5.647 13.300 5.840 2.300 5.977 15.800 6.072 10.100 6.149 5.200 6.511 2.900 6.565 1.600 6.646 10.000 6.866 1.100 7.268 2.800 7.801 4.100 8.455 4.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 18 PeakCount: 17 Return to Lane Navigator
3.500 16.700 4.550 10.700 5.200 10.300 5.850 11.300 6.550 7.700 6.850 6.000 7.350 11.600 8.150 5.000 8.450 6.700 8.650 4.300 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 19 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.